jumpstart taq dna polymerase  (Millipore)


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  • 99
    Name:
    JumpStart Taq DNA Polymerase
    Description:

    Catalog Number:
    d4184
    Price:
    None
    Applications:
    JumpStart(R) Taq DNA Polymerase is used for real time reverse-transcription polymerase chain reaction analysis (Real-Time PCR) in the following ways: . Used for generation of complementary DNAs. Genomic DNA isolation. Isolation of total RNA from cultured cells. IL-4R and IL-13R analysis
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    Structured Review

    Millipore jumpstart taq dna polymerase
    JumpStart Taq DNA Polymerase

    https://www.bioz.com/result/jumpstart taq dna polymerase/product/Millipore
    Average 99 stars, based on 246 article reviews
    Price from $9.99 to $1999.99
    jumpstart taq dna polymerase - by Bioz Stars, 2020-09
    99/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Generation of Human Induced Pluripotent Stem Cells Bearing an Anti-HIV Transgene by a Lentiviral Vector Carrying an Internal Murine Leukemia Virus Promoter
    Article Snippet: .. Provirus-genomic junction fragments were amplified by PCR with primers for HIV gag (5'-GCTCTCGCACCCATCTCTCTCC-3') and Alu (5'-TCCCAGCTACTGGGGAGGCT-3') (1st PCR) using the JumpStart Taq DNA polymerase (D9307, Sigma-Aldrich) with the following cycles: 1 × 94°C for 2 min, 20 × (94°C for 0.5 min, 50°C for 1 min, 72°C for 1.5 min). .. First PCR products were purified with the QIAGEN PCR Purification kit and eluted with 50 μl of water.

    Article Title: New approach to real-time nucleic acids detection: folding polymerase chain reaction amplicons into a secondary structure to improve cleavage of Förster resonance energy transfer probes in 5′-nuclease assays
    Article Snippet: .. PCR reaction components JumpStartTM Taq DNA polymerase, an antibody-inactivated ‘hot start’ enzyme, and dNTPs were purchased from Sigma-Aldrich. .. Primers and FRET probes for Snake, TaqMan, Molecular Beacon and Scorpion assays were prepared by Cepheid (Sunnyvale, CA) using reagents from Glen Research (Sterling, VA).

    Article Title: Myocardial Hypertrophic Remodeling and Impaired Left Ventricular Function in Mice with a Cardiac-Specific Deletion of Janus Kinase 2
    Article Snippet: .. The PCR reaction medium consisted of 10× PCR buffer (Sigma-Aldrich, St. Louis, MO), 0.2 mmol/L dNTPs, 1 μmol/L of each primer, 1 U of Jumpstart RED Taq DNA polymerase (Sigma-Aldrich), and 3 μL of genomic DNA. ..

    Article Title: Developing molecular tools and insights into the Penstemon genome using genomic reduction and next-generation sequencing
    Article Snippet: .. Each 10 μl PCR reaction had ~ 30 ng genomic DNA, 0.05 mM dNTPs, 0.1 mM cresol red, 1.0 μl of 10X PCR buffer (Sigma-Aldrich, St. Louis, MO), 0.5 units of JumpStart™ Taq DNA Polymerase (Sigma-Aldrich, St. Louis, MO) and 0.5 μM (each) of the forward and reverse primers. .. The thermal cycler (Mastercycler® Pro; Eppendorf International; Hamburg, Germany) was set as follows: 94°C for 30 s, 45 cycles of 92°C for 20 s, (primer specific annealing temperature)°C for 1 min. 30 s, 72°C for 2 min., and 72°C for 7 min. (final extension).

    Article Title: Amplification Dynamics of Platy-1 Retrotransposons in the Cebidae Platyrrhine Lineage
    Article Snippet: .. If PCR results were weak or unresolved, the PCR reaction was repeated using hot-start with the JumpStart Taq DNA polymerase kit (Sigma Aldrich). .. Genotypes were recorded in a Microsoft Excel worksheet as (0, 0) homozygous absent, (1, 1) homozygous present, or (1, 0) for heterozygous ( , online).

    Article Title: Bacterial Population Changes in a Membrane Bioreactor for Graywater Treatment Monitored by Denaturing Gradient Gel Electrophoretic Analysis of 16S rRNA Gene Fragments
    Article Snippet: .. The PCR contained 0.3 U of either Taq polymerase (Promega, Madison, Wis.) or JumpStart Taq polymerase (Sigma-Aldrich, St. Louis, Mo.), the appropriate 1× PCR buffer (Promega or Sigma-Aldrich), 3.5 mM MgCl2 , 0.1% bovine serum albumin, 0.2 mM deoxynucleoside triphosphates (dNTPs), and 8 μM each forward and reverse primers. .. The forward primer sequence, not including the 40-nucleotide GC clamp ( ) on the 5′ end, was 5′-CCTACGGGAGGCAGCAG-3′.

    Article Title: Ablation of Doublecortin-Like Kinase 1 in the Colonic Epithelium Exacerbates Dextran Sulfate Sodium-Induced Colitis
    Article Snippet: .. The complementary DNA (cDNA) was subsequently used to perform real-time polymerase chain reaction (PCR) by SYBR chemistry (SYBR Green I, Molecular Probes, Eugene, OR) for specific transcripts using gene-specific primers and JumpStart Taq DNA polymerase (Sigma-Aldrich, St. Louis, MO). .. The crossing threshold value assessed by real-time PCR was noted for the transcripts and normalized with β-actin messenger RNA (mRNA).

    Real-time Polymerase Chain Reaction:

    Article Title: Ablation of Doublecortin-Like Kinase 1 in the Colonic Epithelium Exacerbates Dextran Sulfate Sodium-Induced Colitis
    Article Snippet: .. The complementary DNA (cDNA) was subsequently used to perform real-time polymerase chain reaction (PCR) by SYBR chemistry (SYBR Green I, Molecular Probes, Eugene, OR) for specific transcripts using gene-specific primers and JumpStart Taq DNA polymerase (Sigma-Aldrich, St. Louis, MO). .. The crossing threshold value assessed by real-time PCR was noted for the transcripts and normalized with β-actin messenger RNA (mRNA).

    SYBR Green Assay:

    Article Title: Ablation of Doublecortin-Like Kinase 1 in the Colonic Epithelium Exacerbates Dextran Sulfate Sodium-Induced Colitis
    Article Snippet: .. The complementary DNA (cDNA) was subsequently used to perform real-time polymerase chain reaction (PCR) by SYBR chemistry (SYBR Green I, Molecular Probes, Eugene, OR) for specific transcripts using gene-specific primers and JumpStart Taq DNA polymerase (Sigma-Aldrich, St. Louis, MO). .. The crossing threshold value assessed by real-time PCR was noted for the transcripts and normalized with β-actin messenger RNA (mRNA).

    Amplification:

    Article Title: Generation of Human Induced Pluripotent Stem Cells Bearing an Anti-HIV Transgene by a Lentiviral Vector Carrying an Internal Murine Leukemia Virus Promoter
    Article Snippet: .. Provirus-genomic junction fragments were amplified by PCR with primers for HIV gag (5'-GCTCTCGCACCCATCTCTCTCC-3') and Alu (5'-TCCCAGCTACTGGGGAGGCT-3') (1st PCR) using the JumpStart Taq DNA polymerase (D9307, Sigma-Aldrich) with the following cycles: 1 × 94°C for 2 min, 20 × (94°C for 0.5 min, 50°C for 1 min, 72°C for 1.5 min). .. First PCR products were purified with the QIAGEN PCR Purification kit and eluted with 50 μl of water.

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  • 94
    Millipore real time qpcr assay
    Comparison of B. tryoni LAMP and B. tryoni real-time <t>qPCR</t> assays. ( a ) LAMP, <t>DNA</t> dilution series amplification times for two biological replicates of B. tryoni (triangles). ( b ) Real-time qPCR, DNA dilution series Cq values, DNA samples as above. ( c ) Direct comparison of new LAMP assays and qPCR assays on dilution series DNA, symbols represent average replicates of B. tryoni (circles), linear regression R 2 = 0.91.
    Real Time Qpcr Assay, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 0 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/real time qpcr assay/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    real time qpcr assay - by Bioz Stars, 2020-09
    94/100 stars
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    96
    Millipore sybr green jumpstart taq readymix for high throughput qpcr
    Comparison of B. tryoni LAMP and B. tryoni real-time <t>qPCR</t> assays. ( a ) LAMP, <t>DNA</t> dilution series amplification times for two biological replicates of B. tryoni (triangles). ( b ) Real-time qPCR, DNA dilution series Cq values, DNA samples as above. ( c ) Direct comparison of new LAMP assays and qPCR assays on dilution series DNA, symbols represent average replicates of B. tryoni (circles), linear regression R 2 = 0.91.
    Sybr Green Jumpstart Taq Readymix For High Throughput Qpcr, supplied by Millipore, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/sybr green jumpstart taq readymix for high throughput qpcr/product/Millipore
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    sybr green jumpstart taq readymix for high throughput qpcr - by Bioz Stars, 2020-09
    96/100 stars
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    94
    Millipore quantitative rt pcr readymix
    Comparison of B. tryoni LAMP and B. tryoni real-time <t>qPCR</t> assays. ( a ) LAMP, <t>DNA</t> dilution series amplification times for two biological replicates of B. tryoni (triangles). ( b ) Real-time qPCR, DNA dilution series Cq values, DNA samples as above. ( c ) Direct comparison of new LAMP assays and qPCR assays on dilution series DNA, symbols represent average replicates of B. tryoni (circles), linear regression R 2 = 0.91.
    Quantitative Rt Pcr Readymix, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantitative rt pcr readymix/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    quantitative rt pcr readymix - by Bioz Stars, 2020-09
    94/100 stars
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    Comparison of B. tryoni LAMP and B. tryoni real-time qPCR assays. ( a ) LAMP, DNA dilution series amplification times for two biological replicates of B. tryoni (triangles). ( b ) Real-time qPCR, DNA dilution series Cq values, DNA samples as above. ( c ) Direct comparison of new LAMP assays and qPCR assays on dilution series DNA, symbols represent average replicates of B. tryoni (circles), linear regression R 2 = 0.91.

    Journal: Scientific Reports

    Article Title: A LAMP assay for the detection of Bactrocera tryoni Queensland fruit fly (Diptera: Tephritidae)

    doi: 10.1038/s41598-020-65715-5

    Figure Lengend Snippet: Comparison of B. tryoni LAMP and B. tryoni real-time qPCR assays. ( a ) LAMP, DNA dilution series amplification times for two biological replicates of B. tryoni (triangles). ( b ) Real-time qPCR, DNA dilution series Cq values, DNA samples as above. ( c ) Direct comparison of new LAMP assays and qPCR assays on dilution series DNA, symbols represent average replicates of B. tryoni (circles), linear regression R 2 = 0.91.

    Article Snippet: The same serial dilution of DNA extracts was also used in real-time qPCR assay.

    Techniques: Real-time Polymerase Chain Reaction, Amplification