jnk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc jnk
    Effects of thrombin/protease-activated receptor 1 on the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NFκB) pathway in astrocytes. (A) Western blot analysis <t>of</t> <t>phosphorylated</t> extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase <t>(JNK),</t> P38 kinase (P38), and NFκB protein levels following astrocyte stimulation with 0, 0.5, 1, or 2 U/mL thrombin for 24 hours. (B–E) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. (F) Western blot analysis of phosphorylated ERK, JNK, P38, and NFκB protein levels following astrocyte stimulation with 1 U/mL thrombin in the presence of 0–3 μM SCH79797 for 24 hours. (G–J) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. Data are expressed as mean ± SEM ( n = 3). * P < 0.05 (one-way analysis of variance followed by Bonferroni’s post hoc comparison test). SCH79797: Protease-activated receptor 1 inhibitor.
    Jnk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Thrombin increases the expression of cholesterol 25-hydroxylase in rat astrocytes after spinal cord injury"

    Article Title: Thrombin increases the expression of cholesterol 25-hydroxylase in rat astrocytes after spinal cord injury

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.357905

    Effects of thrombin/protease-activated receptor 1 on the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NFκB) pathway in astrocytes. (A) Western blot analysis of phosphorylated extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38 kinase (P38), and NFκB protein levels following astrocyte stimulation with 0, 0.5, 1, or 2 U/mL thrombin for 24 hours. (B–E) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. (F) Western blot analysis of phosphorylated ERK, JNK, P38, and NFκB protein levels following astrocyte stimulation with 1 U/mL thrombin in the presence of 0–3 μM SCH79797 for 24 hours. (G–J) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. Data are expressed as mean ± SEM ( n = 3). * P < 0.05 (one-way analysis of variance followed by Bonferroni’s post hoc comparison test). SCH79797: Protease-activated receptor 1 inhibitor.
    Figure Legend Snippet: Effects of thrombin/protease-activated receptor 1 on the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NFκB) pathway in astrocytes. (A) Western blot analysis of phosphorylated extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38 kinase (P38), and NFκB protein levels following astrocyte stimulation with 0, 0.5, 1, or 2 U/mL thrombin for 24 hours. (B–E) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. (F) Western blot analysis of phosphorylated ERK, JNK, P38, and NFκB protein levels following astrocyte stimulation with 1 U/mL thrombin in the presence of 0–3 μM SCH79797 for 24 hours. (G–J) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. Data are expressed as mean ± SEM ( n = 3). * P < 0.05 (one-way analysis of variance followed by Bonferroni’s post hoc comparison test). SCH79797: Protease-activated receptor 1 inhibitor.

    Techniques Used: Western Blot

    jnk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc jnk
    Effects of thrombin/protease-activated receptor 1 on the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NFκB) pathway in astrocytes. (A) Western blot analysis <t>of</t> <t>phosphorylated</t> extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase <t>(JNK),</t> P38 kinase (P38), and NFκB protein levels following astrocyte stimulation with 0, 0.5, 1, or 2 U/mL thrombin for 24 hours. (B–E) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. (F) Western blot analysis of phosphorylated ERK, JNK, P38, and NFκB protein levels following astrocyte stimulation with 1 U/mL thrombin in the presence of 0–3 μM SCH79797 for 24 hours. (G–J) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. Data are expressed as mean ± SEM ( n = 3). * P < 0.05 (one-way analysis of variance followed by Bonferroni’s post hoc comparison test). SCH79797: Protease-activated receptor 1 inhibitor.
    Jnk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jnk/product/Cell Signaling Technology Inc
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    1) Product Images from "Thrombin increases the expression of cholesterol 25-hydroxylase in rat astrocytes after spinal cord injury"

    Article Title: Thrombin increases the expression of cholesterol 25-hydroxylase in rat astrocytes after spinal cord injury

    Journal: Neural Regeneration Research

    doi: 10.4103/1673-5374.357905

    Effects of thrombin/protease-activated receptor 1 on the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NFκB) pathway in astrocytes. (A) Western blot analysis of phosphorylated extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38 kinase (P38), and NFκB protein levels following astrocyte stimulation with 0, 0.5, 1, or 2 U/mL thrombin for 24 hours. (B–E) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. (F) Western blot analysis of phosphorylated ERK, JNK, P38, and NFκB protein levels following astrocyte stimulation with 1 U/mL thrombin in the presence of 0–3 μM SCH79797 for 24 hours. (G–J) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. Data are expressed as mean ± SEM ( n = 3). * P < 0.05 (one-way analysis of variance followed by Bonferroni’s post hoc comparison test). SCH79797: Protease-activated receptor 1 inhibitor.
    Figure Legend Snippet: Effects of thrombin/protease-activated receptor 1 on the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NFκB) pathway in astrocytes. (A) Western blot analysis of phosphorylated extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38 kinase (P38), and NFκB protein levels following astrocyte stimulation with 0, 0.5, 1, or 2 U/mL thrombin for 24 hours. (B–E) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. (F) Western blot analysis of phosphorylated ERK, JNK, P38, and NFκB protein levels following astrocyte stimulation with 1 U/mL thrombin in the presence of 0–3 μM SCH79797 for 24 hours. (G–J) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. Data are expressed as mean ± SEM ( n = 3). * P < 0.05 (one-way analysis of variance followed by Bonferroni’s post hoc comparison test). SCH79797: Protease-activated receptor 1 inhibitor.

    Techniques Used: Western Blot

    phosphorylated c jun n terminal kinase  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc phosphorylated c jun n terminal kinase
    Phosphorylated C Jun N Terminal Kinase, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti jnk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc rabbit anti jnk
    Rabbit Anti Jnk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    rabbit anti phospho jnk  (Cell Signaling Technology Inc)


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    p jnk thr183 tyr185  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p jnk thr183 tyr185
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    jnk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc jnk
    Effects of montmorillonite (Mt) on the MAPK signaling pathway in vitro and in vivo. A , B Representative bands of the western blot of <t>JNK,</t> p-JNK, ERK1/2, <t>p-ERK1/2,</t> <t>p38,</t> and p-p38 in the total proteins. Total proteins were extracted from HCEC-B4G12 cells exposed to various concentrations (1.56–100 μg/mL) of Na-Mt for 12 h and 24 h. C , D Quantification of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in A , B according to the densitometric analysis. Intensities of bands were normalized to the amount of GAPDH. *p < 0.05 between the Na-Mt treatment and the control. E – J Representative images indicating the expression of p-JNK ( E , red), JNK ( F , green), p-ERK1/2 ( G , red), ERK1/2 ( H , green), p-p38 ( I , red), and p38 ( J , green), in the cornea. Corneal sections were prepared from rats after exposing them to different concentrations (2 and 10 mg/mL) of Na-Mt or C-H-Na-Mt for 7 days. Nuclei were stained by DAPI (blue). ( K , L ) Quantified fluorescence intensity of the proteins in ( E – J ). *p < 0.05 compared with the control. Scale bars: 50 μm
    Jnk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "ROS generation and p-38 activation contribute to montmorillonite-induced corneal toxicity in vitro and in vivo"

    Article Title: ROS generation and p-38 activation contribute to montmorillonite-induced corneal toxicity in vitro and in vivo

    Journal: Particle and Fibre Toxicology

    doi: 10.1186/s12989-023-00519-9

    Effects of montmorillonite (Mt) on the MAPK signaling pathway in vitro and in vivo. A , B Representative bands of the western blot of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in the total proteins. Total proteins were extracted from HCEC-B4G12 cells exposed to various concentrations (1.56–100 μg/mL) of Na-Mt for 12 h and 24 h. C , D Quantification of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in A , B according to the densitometric analysis. Intensities of bands were normalized to the amount of GAPDH. *p < 0.05 between the Na-Mt treatment and the control. E – J Representative images indicating the expression of p-JNK ( E , red), JNK ( F , green), p-ERK1/2 ( G , red), ERK1/2 ( H , green), p-p38 ( I , red), and p38 ( J , green), in the cornea. Corneal sections were prepared from rats after exposing them to different concentrations (2 and 10 mg/mL) of Na-Mt or C-H-Na-Mt for 7 days. Nuclei were stained by DAPI (blue). ( K , L ) Quantified fluorescence intensity of the proteins in ( E – J ). *p < 0.05 compared with the control. Scale bars: 50 μm
    Figure Legend Snippet: Effects of montmorillonite (Mt) on the MAPK signaling pathway in vitro and in vivo. A , B Representative bands of the western blot of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in the total proteins. Total proteins were extracted from HCEC-B4G12 cells exposed to various concentrations (1.56–100 μg/mL) of Na-Mt for 12 h and 24 h. C , D Quantification of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in A , B according to the densitometric analysis. Intensities of bands were normalized to the amount of GAPDH. *p < 0.05 between the Na-Mt treatment and the control. E – J Representative images indicating the expression of p-JNK ( E , red), JNK ( F , green), p-ERK1/2 ( G , red), ERK1/2 ( H , green), p-p38 ( I , red), and p38 ( J , green), in the cornea. Corneal sections were prepared from rats after exposing them to different concentrations (2 and 10 mg/mL) of Na-Mt or C-H-Na-Mt for 7 days. Nuclei were stained by DAPI (blue). ( K , L ) Quantified fluorescence intensity of the proteins in ( E – J ). *p < 0.05 compared with the control. Scale bars: 50 μm

    Techniques Used: In Vitro, In Vivo, Western Blot, Expressing, Staining, Fluorescence

    Effects of NAC pretreatment on the MAPK signaling pathway. A , B HCEC-B4G12 cells were pretreated with 10 μM SB203580 (p38 inhibitor) for 2 h prior to a treatment with Na-Mt for additional 24 h. The expression levels of p38 and p-p38 were investigated by western blot analysis ( A ). ATP content was measured by the CellTiter-Lum Plus Luminescent Cell Viability Assay ( B ). C Representative western bands of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in HCEC-B4G12. HCEC-B4G12 cells were pretreated with 10 mM NAC for 1 h before exposing them to 25 μg/mL Na-Mt for an additional 24 h. GAPDH is the loading control ( A , C ). Data points are the mean ± SD from three independent experiments, with three parallel samples per concentration in each experiment. *p < 0.05 compared with the control lacking the SB203580 pretreatment. # p < 0.05 compared with the control receiving the SB203580 pretreatment. & p < 0.05 between treatments with and without SB203580 pretreatment at the same concentration of Na-Mt
    Figure Legend Snippet: Effects of NAC pretreatment on the MAPK signaling pathway. A , B HCEC-B4G12 cells were pretreated with 10 μM SB203580 (p38 inhibitor) for 2 h prior to a treatment with Na-Mt for additional 24 h. The expression levels of p38 and p-p38 were investigated by western blot analysis ( A ). ATP content was measured by the CellTiter-Lum Plus Luminescent Cell Viability Assay ( B ). C Representative western bands of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in HCEC-B4G12. HCEC-B4G12 cells were pretreated with 10 mM NAC for 1 h before exposing them to 25 μg/mL Na-Mt for an additional 24 h. GAPDH is the loading control ( A , C ). Data points are the mean ± SD from three independent experiments, with three parallel samples per concentration in each experiment. *p < 0.05 compared with the control lacking the SB203580 pretreatment. # p < 0.05 compared with the control receiving the SB203580 pretreatment. & p < 0.05 between treatments with and without SB203580 pretreatment at the same concentration of Na-Mt

    Techniques Used: Expressing, Western Blot, Cell Viability Assay, Concentration Assay

    p jnk thr183 tyr185  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc p jnk thr183 tyr185
    P Jnk Thr183 Tyr185, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    jnk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc jnk
    Effects of montmorillonite (Mt) on the MAPK signaling pathway in vitro and in vivo. A , B Representative bands of the western blot of <t>JNK,</t> p-JNK, ERK1/2, <t>p-ERK1/2,</t> <t>p38,</t> and p-p38 in the total proteins. Total proteins were extracted from HCEC-B4G12 cells exposed to various concentrations (1.56–100 μg/mL) of Na-Mt for 12 h and 24 h. C , D Quantification of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in A , B according to the densitometric analysis. Intensities of bands were normalized to the amount of GAPDH. *p < 0.05 between the Na-Mt treatment and the control. E – J Representative images indicating the expression of p-JNK ( E , red), JNK ( F , green), p-ERK1/2 ( G , red), ERK1/2 ( H , green), p-p38 ( I , red), and p38 ( J , green), in the cornea. Corneal sections were prepared from rats after exposing them to different concentrations (2 and 10 mg/mL) of Na-Mt or C-H-Na-Mt for 7 days. Nuclei were stained by DAPI (blue). ( K , L ) Quantified fluorescence intensity of the proteins in ( E – J ). *p < 0.05 compared with the control. Scale bars: 50 μm
    Jnk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jnk/product/Cell Signaling Technology Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jnk - by Bioz Stars, 2023-03
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    Images

    1) Product Images from "ROS generation and p-38 activation contribute to montmorillonite-induced corneal toxicity in vitro and in vivo"

    Article Title: ROS generation and p-38 activation contribute to montmorillonite-induced corneal toxicity in vitro and in vivo

    Journal: Particle and Fibre Toxicology

    doi: 10.1186/s12989-023-00519-9

    Effects of montmorillonite (Mt) on the MAPK signaling pathway in vitro and in vivo. A , B Representative bands of the western blot of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in the total proteins. Total proteins were extracted from HCEC-B4G12 cells exposed to various concentrations (1.56–100 μg/mL) of Na-Mt for 12 h and 24 h. C , D Quantification of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in A , B according to the densitometric analysis. Intensities of bands were normalized to the amount of GAPDH. *p < 0.05 between the Na-Mt treatment and the control. E – J Representative images indicating the expression of p-JNK ( E , red), JNK ( F , green), p-ERK1/2 ( G , red), ERK1/2 ( H , green), p-p38 ( I , red), and p38 ( J , green), in the cornea. Corneal sections were prepared from rats after exposing them to different concentrations (2 and 10 mg/mL) of Na-Mt or C-H-Na-Mt for 7 days. Nuclei were stained by DAPI (blue). ( K , L ) Quantified fluorescence intensity of the proteins in ( E – J ). *p < 0.05 compared with the control. Scale bars: 50 μm
    Figure Legend Snippet: Effects of montmorillonite (Mt) on the MAPK signaling pathway in vitro and in vivo. A , B Representative bands of the western blot of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in the total proteins. Total proteins were extracted from HCEC-B4G12 cells exposed to various concentrations (1.56–100 μg/mL) of Na-Mt for 12 h and 24 h. C , D Quantification of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in A , B according to the densitometric analysis. Intensities of bands were normalized to the amount of GAPDH. *p < 0.05 between the Na-Mt treatment and the control. E – J Representative images indicating the expression of p-JNK ( E , red), JNK ( F , green), p-ERK1/2 ( G , red), ERK1/2 ( H , green), p-p38 ( I , red), and p38 ( J , green), in the cornea. Corneal sections were prepared from rats after exposing them to different concentrations (2 and 10 mg/mL) of Na-Mt or C-H-Na-Mt for 7 days. Nuclei were stained by DAPI (blue). ( K , L ) Quantified fluorescence intensity of the proteins in ( E – J ). *p < 0.05 compared with the control. Scale bars: 50 μm

    Techniques Used: In Vitro, In Vivo, Western Blot, Expressing, Staining, Fluorescence

    Effects of NAC pretreatment on the MAPK signaling pathway. A , B HCEC-B4G12 cells were pretreated with 10 μM SB203580 (p38 inhibitor) for 2 h prior to a treatment with Na-Mt for additional 24 h. The expression levels of p38 and p-p38 were investigated by western blot analysis ( A ). ATP content was measured by the CellTiter-Lum Plus Luminescent Cell Viability Assay ( B ). C Representative western bands of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in HCEC-B4G12. HCEC-B4G12 cells were pretreated with 10 mM NAC for 1 h before exposing them to 25 μg/mL Na-Mt for an additional 24 h. GAPDH is the loading control ( A , C ). Data points are the mean ± SD from three independent experiments, with three parallel samples per concentration in each experiment. *p < 0.05 compared with the control lacking the SB203580 pretreatment. # p < 0.05 compared with the control receiving the SB203580 pretreatment. & p < 0.05 between treatments with and without SB203580 pretreatment at the same concentration of Na-Mt
    Figure Legend Snippet: Effects of NAC pretreatment on the MAPK signaling pathway. A , B HCEC-B4G12 cells were pretreated with 10 μM SB203580 (p38 inhibitor) for 2 h prior to a treatment with Na-Mt for additional 24 h. The expression levels of p38 and p-p38 were investigated by western blot analysis ( A ). ATP content was measured by the CellTiter-Lum Plus Luminescent Cell Viability Assay ( B ). C Representative western bands of JNK, p-JNK, ERK1/2, p-ERK1/2, p38, and p-p38 in HCEC-B4G12. HCEC-B4G12 cells were pretreated with 10 mM NAC for 1 h before exposing them to 25 μg/mL Na-Mt for an additional 24 h. GAPDH is the loading control ( A , C ). Data points are the mean ± SD from three independent experiments, with three parallel samples per concentration in each experiment. *p < 0.05 compared with the control lacking the SB203580 pretreatment. # p < 0.05 compared with the control receiving the SB203580 pretreatment. & p < 0.05 between treatments with and without SB203580 pretreatment at the same concentration of Na-Mt

    Techniques Used: Expressing, Western Blot, Cell Viability Assay, Concentration Assay

    anti sapk jnk  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti sapk jnk
    Anti Sapk Jnk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    anti phospho sapk jnk thr183 tyr185  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc anti phospho sapk jnk thr183 tyr185
    Anti Phospho Sapk Jnk Thr183 Tyr185, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Effects of thrombin/protease-activated receptor 1 on the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NFκB) pathway in astrocytes. (A) Western blot analysis of phosphorylated extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38 kinase (P38), and NFκB protein levels following astrocyte stimulation with 0, 0.5, 1, or 2 U/mL thrombin for 24 hours. (B–E) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. (F) Western blot analysis of phosphorylated ERK, JNK, P38, and NFκB protein levels following astrocyte stimulation with 1 U/mL thrombin in the presence of 0–3 μM SCH79797 for 24 hours. (G–J) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. Data are expressed as mean ± SEM ( n = 3). * P < 0.05 (one-way analysis of variance followed by Bonferroni’s post hoc comparison test). SCH79797: Protease-activated receptor 1 inhibitor.

    Journal: Neural Regeneration Research

    Article Title: Thrombin increases the expression of cholesterol 25-hydroxylase in rat astrocytes after spinal cord injury

    doi: 10.4103/1673-5374.357905

    Figure Lengend Snippet: Effects of thrombin/protease-activated receptor 1 on the mitogen-activated protein kinase (MAPK)/nuclear factor kappa-B (NFκB) pathway in astrocytes. (A) Western blot analysis of phosphorylated extracellular regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), P38 kinase (P38), and NFκB protein levels following astrocyte stimulation with 0, 0.5, 1, or 2 U/mL thrombin for 24 hours. (B–E) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. (F) Western blot analysis of phosphorylated ERK, JNK, P38, and NFκB protein levels following astrocyte stimulation with 1 U/mL thrombin in the presence of 0–3 μM SCH79797 for 24 hours. (G–J) Quantification of phosphorylated ERK, JNK, P38, and NFκB protein levels, normalized to β-actin. Data are expressed as mean ± SEM ( n = 3). * P < 0.05 (one-way analysis of variance followed by Bonferroni’s post hoc comparison test). SCH79797: Protease-activated receptor 1 inhibitor.

    Article Snippet: After blocking with 5% skim milk for 1 hour, the membrane was incubated with the following primary antibodies overnight at 4°C: CH25H (rabbit, 1:500, Invitrogen, Cat# PA5-70691, RRID: AB_2689560), phosphorylated extracellular signal regulated kinase (pERK)1/2 (rabbit, 1:1000, CST, Cat# 9102S, RRID: AB_330744), extracellular signal regulated kinase (ERK)1/2 (rabbit, 1:1000, CST, Cat# 8544S, RRID: AB_11127856), phosphorylated c-Jun N-terminal kinase (pJNK; rabbit, 1:1000, CST, Cat# 9251S, RRID: AB_331659), JNK (rabbit, 1:1000, CST, Cat# 9252S, RRID: AB_2250373), phosphorylated P38 kinase (pP38; rabbit, 1:1000, CST, Cat# 8632S, RRID: AB_2797648), P38 (rabbit, 1:1000, CST, Cat# 14451S, RRID: AB_2798482), nuclear factor kappa-B (NFκB; rabbit, 1:1000, CST, Cat# 12629S, RRID: AB_2722509), and β-actin (mouse, 1:5000, CST, Cat# 3700S, RRID: AB_2242334).

    Techniques: Western Blot