jbscreen classic hts i  (Jena Bioscience)

Journal: Acta Crystallographica. Section D, Structural Biology

Article Title: A standardized technique for high-pressure cooling of protein crystals

doi: 10.1107/S2059798317016357

Figure Lengend Snippet: Survey of the cooling tests. Illustrated are the three crystallization screens tested: JBScreen Classic HTS I (Jena Bioscience; top), The JCSG Core Suite I (Qiagen; middle) and MemGold ECO (Molecular Dimensions; bottom). On the left are the HPC results: ( a ), ( b ) and ( c ). On the right are the results for gas-stream cooling: ( d ), ( e ) and ( f ). Boxes corresponding to the respective solutions were filled with the count of samples that formed amorphous ice during the cooling tests. Boxes without numbers were extrapolated from the screening results. The boxes were marked in orange if one or more trials out of three replicates were not cooled successfully. Where two out of three samples showed amorphous ice, boxes were coloured light green (HPC) and light blue (gas stream). For all solutions which showed amorphous ice in all samples the boxes were coloured green (HPC) and blue (gas stream).

Article Snippet: The screens were The JCSG Core Suite I (Qiagen), MemGold ECO (Molecular Dimensions) and JBScreen Classic HTS I (Jena Bioscience).

Techniques: Crystallization Assay

Journal: Biochemistry and Biophysics Reports

Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

doi: 10.1016/j.bbrep.2018.09.005

Figure Lengend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

Article Snippet: It is important to mention that we were also able to grow LOV-HK crystals in the dark following a strict light control protocol and in the same condition that yielded Screen hit #2 for the illuminated protein (5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5, Jena Bioscience JBScreen Classic 9 Solution A1), but unfortunately the best diffraction signal obtained for these samples reached only 6 Å resolution.

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

doi: 10.1016/j.bbrep.2018.09.005

Figure Lengend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

Article Snippet: It is important to mention that we were also able to grow LOV-HK crystals in the dark following a strict light control protocol and in the same condition that yielded Screen hit #2 for the illuminated protein (5% (v/v ) isopropanol, 0.1 M Hepes, pH 7.5, Jena Bioscience JBScreen Classic 9 Solution A1), but unfortunately the best diffraction signal obtained for these samples reached only 6 Å resolution.

Techniques:

Journal: Biochemistry and Biophysics Reports

Article Title: Crystallization and initial X-ray diffraction analysis of the multi-domain Brucella blue light-activated histidine kinase LOV-HK in its illuminated state

doi: 10.1016/j.bbrep.2018.09.005

Figure Lengend Snippet: LOV-HK crystals. (A) Screen hit #1, obtained with 30% ( v/v ) MPD, 5% ( w/v ) PEG 4000, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 7 Solution C4). (B) Screen hit #2, obtained with 5% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 9 Solution A1). (C) Screen hit #3, obtained with 10% ( w/v ) PEG 4000, 20% ( v/v ) isopropanol, 0.1 M Hepes, pH 7.5 (Jena Bioscience JBScreen Classic 3 Solution A5). (D) Improved crystals obtained after optimization of screen hit #1. (E) Mounted crystal at the PROXIMA-2A beamline, ready for diffraction.

Article Snippet: Initial crystallization trials were performed at 5.3 mg ml−1 in 96-well sitting-drop vapour-diffusion Greiner 609120 plates (Monroe, North Carolina, USA) using a Honeybee 963 robot (Digilab, Marlborough, Massachusetts, USA) and the following crystallization kits: Jena Bioscience JBScreen Classic and Pentaerythritol (Jena, Germany), and Hampton Research Crystal Screen, Crystal Screen 2, PEG/Ion, PEG/Ion 2, PEGRx 1 and PEGRx 2 (Aliso Viejo, California, USA).

Techniques: