jbscreen classic kits  (Jena Bioscience)


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    Jena Bioscience jbscreen classic kits
    Jbscreen Classic Kits, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jbscreen classic kits - by Bioz Stars, 2022-10
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    Jena Bioscience atto425 protein labeling kit
    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA <t>labeled</t> with an <t>Atto425.</t> TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of
    Atto425 Protein Labeling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atto425 protein labeling kit/product/Jena Bioscience
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    atto425 protein labeling kit - by Bioz Stars, 2022-10
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    95
    Jena Bioscience pcr purification kit
    Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-16 (457bp) and 18 (322bp) primers. Numbers are samples ID; L is a mid-range ladder (Jena Bioscience); NC is the negative control while PC is the positive control. The sizes of the <t>PCR</t> products generated are 457bp for HPV-16 and 322bp for HPV-18.
    Pcr Purification Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/pcr purification kit/product/Jena Bioscience
    Average 95 stars, based on 1 article reviews
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    94
    Jena Bioscience highyield t7 cy3 rna labeling kit
    Ubx binds <t>RNA</t> in vivo and in vitro , partly via its Homeodomain. (A–C) RNA-immunoprecipitation (RIP-RT-qPCR) experiments of Drosophila S2R+ cells expressing GFP control (white), GFP-Ubx WT (blue) and GFP-Ubx N51A (purple) showing an enrichment of targeted exonic regions of Chas ( A ), pAbp ( B ) and Rgk1 ( C ). Values are RNA relative enrichment over GFP calculated as percentage of input. The input represents the total RNA detected in each sample and thus, normalises the enrichment to the total RNA expression level. (E + number) = exon related to JunctionSeq annotation, differentially spliced exons are underlined (purple). n = 3 independent biological duplicates. Bars represent mean ± SEM. The results exhibited a specific enrichment of differentially spliced exonic sequences in Ubx WT fraction compared to GFP and Ubx N51A . ( D–K ) Fluorescent protein-RNA interaction assay followed by UV-crosslinking and RNase digestion, performed in vitro with purified proteins namely his-MBP-GFP as control, his-Ubx (WT and N51A) full-length (FL) ( D–G ) and the HD alone his-MBP-Ubx HD (WT and N51A) ( H–K ). Interactions were detected on denaturating gels by <t>Cy3-UTP</t> signal (upper panel), and gels were stained by Coomassie to reveal the protein content (lower panel). Each probe is indicated relative to the genes and exons. Molecular marker is indicated showing the size of each protein. MBP fused proteins are named his-MBP-X, his-fused proteins are named his-X. (L–O) Graphical view showing the quantification of relative RNA-binding of Ubx HD compared to full-length (FL) MBP fused proteins for Chas exon cassette E5 ( L ), constitutive E3, ( M ), pAbp exon cassette E1 ( N ), constitutive E6 ( O ) normalised to Coomassie staining. n = 3 independent biological replicates. Statistical test by one-way ANOVA (* P
    Highyield T7 Cy3 Rna Labeling Kit, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Jena Bioscience atto647n
    Single-molecule colocalization of NAC and SRP on surface immobilized RNCs. a – d Representative traces of NAC-SRP colocalization on surface immobilized RNC(ss) ( a , b ) and RNC(ssmt) ( c , d ). Biotinylated quartz surface was coated with 1.5 nM purified monosome RNC(ss) or RNC(ssmt) with 3’ biotinylated mRNA. The sample chamber was then flushed with 2 nM NAC Cy3B and 1 nM SRP <t>Atto647n</t> (labeled at SRP54-S12C) in image buffer. Movies were recorded in ALEX mode for 60 s at a speed of 10 frames per second. Note the anti-correlation in A em – D ex and D em – D ex panels that indicates FRET between NAC Cy3B and SRP Atto647n . e – g Dwell time analysis of the NAC binding events to RNC(ss) ( e ) and RNC(ssmt) ( f ). Single molecule fluorescence traces were fit to a Hidden Markov Model (HMM) to extract dwell times of NAC in the colocalized state. The cumulative distributions of dwell times were fitted to a double-exponential function Eq. ( 17 ) in the Method, and the fitted parameters are reported in ( g ). Number of transition is the total number of transitions observed for NAC binding to and dissociation from the RNC-SRP complex. h Summary of the frequency of observed SRP-NAC colocalization events under each condition, calculated from the ratio of the number of acceptors with colocalized donor over the total number of acceptors detected. No RNC indicates that 2 nM NAC Cy3B and 1 nM SRP Atto647n (labeled at 54-S12C) were incubated in image buffer on microscope slides without immobilized RNC. RNC(ss) w/ NACmt is the same as RNC(ss) except that 2 nM of NACmt instead of NAC was used.
    Atto647n, supplied by Jena Bioscience, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/atto647n/product/Jena Bioscience
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    Image Search Results


    ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Overexpression of the Endosomal Anion/Proton Exchanger ClC-5 Increases Cell Susceptibility toward Clostridium difficile Toxins TcdA and TcdB

    doi: 10.3389/fcimb.2017.00067

    Figure Lengend Snippet: ClC-5 and ClC-5 E268Q enhance the internalization of TcdA . HEK293 cells stably transfected with the fluorescent mCherry (blue), ClC-5-mCherry (WT, black) and ClC-5-mCherry E268Q (red) were treated for 45 min on ice followed by a 5-min incubation at 37°C with 200 nM TcdA labeled with an Atto425. TcdA uptake was assessed by the Atto425 fluorescence intensity excited at 425 nm and measured at 440–650 nm. To correct for the different number of cells in the individual experiments, cell density was additionally measured using by absorbance spectrophotometry (Supplementary Figure S5 ). 9 Petri dishes were analyzed for ClC-5 WT and E268Q and 10 for mCherry on two different days. Bars represent means ±SEM at 490 nm emission. * Indicates a significant difference between two data sets. The significance was tested using a two-tailed t -test and significance was set at a P -value of

    Article Snippet: To measure the amount of internalized TcdA, the primary amino groups of full-length TcdA were labeled using the Atto425 Protein Labeling Kit (Jena Bioscience GmbH) according to the manufacturer's protocol.

    Techniques: Stable Transfection, Transfection, Incubation, Labeling, Fluorescence, Spectrophotometry, Two Tailed Test

    Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-16 (457bp) and 18 (322bp) primers. Numbers are samples ID; L is a mid-range ladder (Jena Bioscience); NC is the negative control while PC is the positive control. The sizes of the PCR products generated are 457bp for HPV-16 and 322bp for HPV-18.

    Journal: PLoS ONE

    Article Title: Molecular characterisation of genital human papillomavirus among women in Southwestern, Nigeria

    doi: 10.1371/journal.pone.0224748

    Figure Lengend Snippet: Agarose gel electrophoresis Image of HPV DNA genotyping with HPV-16 (457bp) and 18 (322bp) primers. Numbers are samples ID; L is a mid-range ladder (Jena Bioscience); NC is the negative control while PC is the positive control. The sizes of the PCR products generated are 457bp for HPV-16 and 322bp for HPV-18.

    Article Snippet: The PCR products were purified with a commercially available PCR purification kit (Jena Bioscience, Germany) according to the manufacturer’s instructions.

    Techniques: Agarose Gel Electrophoresis, Negative Control, Positive Control, Polymerase Chain Reaction, Generated

    Ubx binds RNA in vivo and in vitro , partly via its Homeodomain. (A–C) RNA-immunoprecipitation (RIP-RT-qPCR) experiments of Drosophila S2R+ cells expressing GFP control (white), GFP-Ubx WT (blue) and GFP-Ubx N51A (purple) showing an enrichment of targeted exonic regions of Chas ( A ), pAbp ( B ) and Rgk1 ( C ). Values are RNA relative enrichment over GFP calculated as percentage of input. The input represents the total RNA detected in each sample and thus, normalises the enrichment to the total RNA expression level. (E + number) = exon related to JunctionSeq annotation, differentially spliced exons are underlined (purple). n = 3 independent biological duplicates. Bars represent mean ± SEM. The results exhibited a specific enrichment of differentially spliced exonic sequences in Ubx WT fraction compared to GFP and Ubx N51A . ( D–K ) Fluorescent protein-RNA interaction assay followed by UV-crosslinking and RNase digestion, performed in vitro with purified proteins namely his-MBP-GFP as control, his-Ubx (WT and N51A) full-length (FL) ( D–G ) and the HD alone his-MBP-Ubx HD (WT and N51A) ( H–K ). Interactions were detected on denaturating gels by Cy3-UTP signal (upper panel), and gels were stained by Coomassie to reveal the protein content (lower panel). Each probe is indicated relative to the genes and exons. Molecular marker is indicated showing the size of each protein. MBP fused proteins are named his-MBP-X, his-fused proteins are named his-X. (L–O) Graphical view showing the quantification of relative RNA-binding of Ubx HD compared to full-length (FL) MBP fused proteins for Chas exon cassette E5 ( L ), constitutive E3, ( M ), pAbp exon cassette E1 ( N ), constitutive E6 ( O ) normalised to Coomassie staining. n = 3 independent biological replicates. Statistical test by one-way ANOVA (* P

    Journal: Nucleic Acids Research

    Article Title: The Hox transcription factor Ultrabithorax binds RNA and regulates co-transcriptional splicing through an interplay with RNA polymerase II

    doi: 10.1093/nar/gkab1250

    Figure Lengend Snippet: Ubx binds RNA in vivo and in vitro , partly via its Homeodomain. (A–C) RNA-immunoprecipitation (RIP-RT-qPCR) experiments of Drosophila S2R+ cells expressing GFP control (white), GFP-Ubx WT (blue) and GFP-Ubx N51A (purple) showing an enrichment of targeted exonic regions of Chas ( A ), pAbp ( B ) and Rgk1 ( C ). Values are RNA relative enrichment over GFP calculated as percentage of input. The input represents the total RNA detected in each sample and thus, normalises the enrichment to the total RNA expression level. (E + number) = exon related to JunctionSeq annotation, differentially spliced exons are underlined (purple). n = 3 independent biological duplicates. Bars represent mean ± SEM. The results exhibited a specific enrichment of differentially spliced exonic sequences in Ubx WT fraction compared to GFP and Ubx N51A . ( D–K ) Fluorescent protein-RNA interaction assay followed by UV-crosslinking and RNase digestion, performed in vitro with purified proteins namely his-MBP-GFP as control, his-Ubx (WT and N51A) full-length (FL) ( D–G ) and the HD alone his-MBP-Ubx HD (WT and N51A) ( H–K ). Interactions were detected on denaturating gels by Cy3-UTP signal (upper panel), and gels were stained by Coomassie to reveal the protein content (lower panel). Each probe is indicated relative to the genes and exons. Molecular marker is indicated showing the size of each protein. MBP fused proteins are named his-MBP-X, his-fused proteins are named his-X. (L–O) Graphical view showing the quantification of relative RNA-binding of Ubx HD compared to full-length (FL) MBP fused proteins for Chas exon cassette E5 ( L ), constitutive E3, ( M ), pAbp exon cassette E1 ( N ), constitutive E6 ( O ) normalised to Coomassie staining. n = 3 independent biological replicates. Statistical test by one-way ANOVA (* P

    Article Snippet: For producing internally labelled RNA, in vitro transcription was performed using the HighYield T7 Cy3 RNA Labelling Kit (Jena Bioscience, RNT-101-CY3) in accordance to the instructions of the manufacturer.

    Techniques: In Vivo, In Vitro, Immunoprecipitation, Quantitative RT-PCR, Expressing, RNA Expression, Purification, Staining, Marker, RNA Binding Assay

    Single-molecule colocalization of NAC and SRP on surface immobilized RNCs. a – d Representative traces of NAC-SRP colocalization on surface immobilized RNC(ss) ( a , b ) and RNC(ssmt) ( c , d ). Biotinylated quartz surface was coated with 1.5 nM purified monosome RNC(ss) or RNC(ssmt) with 3’ biotinylated mRNA. The sample chamber was then flushed with 2 nM NAC Cy3B and 1 nM SRP Atto647n (labeled at SRP54-S12C) in image buffer. Movies were recorded in ALEX mode for 60 s at a speed of 10 frames per second. Note the anti-correlation in A em – D ex and D em – D ex panels that indicates FRET between NAC Cy3B and SRP Atto647n . e – g Dwell time analysis of the NAC binding events to RNC(ss) ( e ) and RNC(ssmt) ( f ). Single molecule fluorescence traces were fit to a Hidden Markov Model (HMM) to extract dwell times of NAC in the colocalized state. The cumulative distributions of dwell times were fitted to a double-exponential function Eq. ( 17 ) in the Method, and the fitted parameters are reported in ( g ). Number of transition is the total number of transitions observed for NAC binding to and dissociation from the RNC-SRP complex. h Summary of the frequency of observed SRP-NAC colocalization events under each condition, calculated from the ratio of the number of acceptors with colocalized donor over the total number of acceptors detected. No RNC indicates that 2 nM NAC Cy3B and 1 nM SRP Atto647n (labeled at 54-S12C) were incubated in image buffer on microscope slides without immobilized RNC. RNC(ss) w/ NACmt is the same as RNC(ss) except that 2 nM of NACmt instead of NAC was used.

    Journal: Nature Communications

    Article Title: A ribosome-associated chaperone enables substrate triage in a cotranslational protein targeting complex

    doi: 10.1038/s41467-020-19548-5

    Figure Lengend Snippet: Single-molecule colocalization of NAC and SRP on surface immobilized RNCs. a – d Representative traces of NAC-SRP colocalization on surface immobilized RNC(ss) ( a , b ) and RNC(ssmt) ( c , d ). Biotinylated quartz surface was coated with 1.5 nM purified monosome RNC(ss) or RNC(ssmt) with 3’ biotinylated mRNA. The sample chamber was then flushed with 2 nM NAC Cy3B and 1 nM SRP Atto647n (labeled at SRP54-S12C) in image buffer. Movies were recorded in ALEX mode for 60 s at a speed of 10 frames per second. Note the anti-correlation in A em – D ex and D em – D ex panels that indicates FRET between NAC Cy3B and SRP Atto647n . e – g Dwell time analysis of the NAC binding events to RNC(ss) ( e ) and RNC(ssmt) ( f ). Single molecule fluorescence traces were fit to a Hidden Markov Model (HMM) to extract dwell times of NAC in the colocalized state. The cumulative distributions of dwell times were fitted to a double-exponential function Eq. ( 17 ) in the Method, and the fitted parameters are reported in ( g ). Number of transition is the total number of transitions observed for NAC binding to and dissociation from the RNC-SRP complex. h Summary of the frequency of observed SRP-NAC colocalization events under each condition, calculated from the ratio of the number of acceptors with colocalized donor over the total number of acceptors detected. No RNC indicates that 2 nM NAC Cy3B and 1 nM SRP Atto647n (labeled at 54-S12C) were incubated in image buffer on microscope slides without immobilized RNC. RNC(ss) w/ NACmt is the same as RNC(ss) except that 2 nM of NACmt instead of NAC was used.

    Article Snippet: As previously described , , purified SR with a C-terminal sortase tag was labeled with Atto647n by incubation with purified sortase-A (without a 6× His tag) and dye-conjugated peptide, GGGC-Atto647n, at a molar ratio of 1:4:8 at 25 °C for 4 h in Sortase Buffer (50 mM KHEPES, pH 7.5, 150 mM NaCl, 10 mM CaCl2, 10% glycerol, 2 mM DTT, 0.02% NIKKOL).

    Techniques: Purification, Labeling, Binding Assay, Fluorescence, Incubation, Microscopy