Journal: Acta Crystallographica. Section D, Structural Biology
Article Title: A standardized technique for high-pressure cooling of protein crystals
Figure Lengend Snippet: Survey of the cooling tests. Illustrated are the three crystallization screens tested: JBScreen Classic HTS I (Jena Bioscience; top), The JCSG Core Suite I (Qiagen; middle) and MemGold ECO (Molecular Dimensions; bottom). On the left are the HPC results: ( a ), ( b ) and ( c ). On the right are the results for gas-stream cooling: ( d ), ( e ) and ( f ). Boxes corresponding to the respective solutions were filled with the count of samples that formed amorphous ice during the cooling tests. Boxes without numbers were extrapolated from the screening results. The boxes were marked in orange if one or more trials out of three replicates were not cooled successfully. Where two out of three samples showed amorphous ice, boxes were coloured light green (HPC) and light blue (gas stream). For all solutions which showed amorphous ice in all samples the boxes were coloured green (HPC) and blue (gas stream).
Article Snippet: The screens were The JCSG Core Suite I (Qiagen), MemGold ECO (Molecular Dimensions) and JBScreen Classic HTS I (Jena Bioscience).
Techniques: Crystallization Assay