hplc system  (JASCO Inc)


Bioz Verified Symbol JASCO Inc is a verified supplier
Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    JASCO Inc hplc system
    Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc system - by Bioz Stars, 2024-04
    96/100 stars

    Images

    high performance liquid chromatography hplc jasco lc 4000 series system  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    JASCO Inc high performance liquid chromatography hplc jasco lc 4000 series system
    High Performance Liquid Chromatography Hplc Jasco Lc 4000 Series System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography hplc jasco lc 4000 series system/product/JASCO Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatography hplc jasco lc 4000 series system - by Bioz Stars, 2024-04
    86/100 stars

    Images

    hplc system jasco extrema lc 4000 system  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    JASCO Inc hplc system jasco extrema lc 4000 system
    Hplc System Jasco Extrema Lc 4000 System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system jasco extrema lc 4000 system/product/JASCO Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc system jasco extrema lc 4000 system - by Bioz Stars, 2024-04
    86/100 stars

    Images

    hplc jasco lc 4000 system  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    JASCO Inc hplc jasco lc 4000 system
    (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) <t>SEC-HPLC</t> chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.
    Hplc Jasco Lc 4000 System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc jasco lc 4000 system/product/JASCO Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc jasco lc 4000 system - by Bioz Stars, 2024-04
    86/100 stars

    Images

    1) Product Images from "Development and characterization of DIA 12.3, a fully human intact anti-CEACAM1 monoclonal antibody"

    Article Title: Development and characterization of DIA 12.3, a fully human intact anti-CEACAM1 monoclonal antibody

    Journal: PLOS ONE

    doi: 10.1371/journal.pone.0295345

    (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) SEC-HPLC chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.
    Figure Legend Snippet: (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) SEC-HPLC chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.

    Techniques Used: Plasmid Preparation, Expressing, Amplification, Sequencing, Ligation, Enzyme-linked Immunosorbent Assay, Transfection, Clone Assay, Purification, Molecular Weight, SDS Page

    hplc system  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    JASCO Inc hplc system
    Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc system - by Bioz Stars, 2024-04
    96/100 stars

    Images

    hplc analysis  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    JASCO Inc hplc analysis
    Hplc Analysis, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc analysis/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc analysis - by Bioz Stars, 2024-04
    96/100 stars

    Images

    hplc instrument  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    JASCO Inc hplc instrument
    Hplc Instrument, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc instrument/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc instrument - by Bioz Stars, 2024-04
    96/100 stars

    Images

    preparative hplc  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    JASCO Inc preparative hplc
    Preparative Hplc, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparative hplc/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    preparative hplc - by Bioz Stars, 2024-04
    96/100 stars

    Images

    hplc system  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    JASCO Inc hplc system
    Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc system - by Bioz Stars, 2024-04
    96/100 stars

    Images

    hplc system  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    JASCO Inc hplc system
    Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc system - by Bioz Stars, 2024-04
    96/100 stars

    Images

    hplc system  (JASCO Inc)


    Bioz Verified Symbol JASCO Inc is a verified supplier
    Bioz Manufacturer Symbol JASCO Inc manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96

    Structured Review

    JASCO Inc hplc system
    Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc system - by Bioz Stars, 2024-04
    96/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    JASCO Inc hplc system
    Hplc System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc system - by Bioz Stars, 2024-04
    96/100 stars
      Buy from Supplier

    86
    JASCO Inc high performance liquid chromatography hplc jasco lc 4000 series system
    High Performance Liquid Chromatography Hplc Jasco Lc 4000 Series System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high performance liquid chromatography hplc jasco lc 4000 series system/product/JASCO Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high performance liquid chromatography hplc jasco lc 4000 series system - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    JASCO Inc hplc system jasco extrema lc 4000 system
    Hplc System Jasco Extrema Lc 4000 System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc system jasco extrema lc 4000 system/product/JASCO Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc system jasco extrema lc 4000 system - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    86
    JASCO Inc hplc jasco lc 4000 system
    (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) <t>SEC-HPLC</t> chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.
    Hplc Jasco Lc 4000 System, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc jasco lc 4000 system/product/JASCO Inc
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc jasco lc 4000 system - by Bioz Stars, 2024-04
    86/100 stars
      Buy from Supplier

    96
    JASCO Inc hplc analysis
    (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) <t>SEC-HPLC</t> chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.
    Hplc Analysis, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc analysis/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc analysis - by Bioz Stars, 2024-04
    96/100 stars
      Buy from Supplier

    96
    JASCO Inc hplc instrument
    (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) <t>SEC-HPLC</t> chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.
    Hplc Instrument, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hplc instrument/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    hplc instrument - by Bioz Stars, 2024-04
    96/100 stars
      Buy from Supplier

    96
    JASCO Inc preparative hplc
    (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) <t>SEC-HPLC</t> chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.
    Preparative Hplc, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/preparative hplc/product/JASCO Inc
    Average 96 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    preparative hplc - by Bioz Stars, 2024-04
    96/100 stars
      Buy from Supplier

    Image Search Results


    (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) SEC-HPLC chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.

    Journal: PLOS ONE

    Article Title: Development and characterization of DIA 12.3, a fully human intact anti-CEACAM1 monoclonal antibody

    doi: 10.1371/journal.pone.0295345

    Figure Lengend Snippet: (a) Construction of the bicistronic vector for the antibody expression. Three separate pcDNA 3.1 (-) vectors were used to clone the encoding gene sequences of scFvDiathis LC and IgG1 HC. The expression cassette bearing the CMV promoter, scFvDIATHIS1 LC encoding gene and the polyA chain, was amplified by PCR in order to add the sequence of BglII and NruI restriction sites, upstream and downstream the LC gene, respectively. The PCR product and the vector bearing the IgG1 HC encoding gene were both digested by BglII and Nru restriction enzymes. The digestion was followed by the ligation reaction to finally develop the bicistronic vectors for the simultaneous expression of the LC and the HC. Absorbance values obtained by ELISA assays on 100 μl of the supernatants collected from the transfected cellular pools (b) , clones (c) and subclones (d) for the production of the IgG1 antibody. The statistical significance of the pool differences in the abs values was tested with unpaired t-test. (e) SEC-HPLC chromatogram of IgG1 antibody purified from the subclone #12.3. The molecular weights were calculated based on a standard curve obtained by calibrating the system with molecules of known molecular weight: thyroglobulin 660 kDa, gamma- globulin 150 kDa, ovalbumin 44.3 kDa, ribonuclease A 43.7 kDa, 4-aminobenzoic acid 13.71 kDa. (f) Freshly purified antibody analyzed by SDS-PAGE under reducing (gel on the left) and non-reducing (gel on the right) conditions. (g) SDS- PAGE analysis of the antibody in reducing conditions after eight months of storage at 4°C.

    Article Snippet: Size Exclusion-High Performance Liquid Chromatography (SEC-HPLC) was performed with the HPLC Jasco LC-4000 system using a TSK Gel G3000SW xL L x I.D. column equilibrated with 0.1mol/L Na 2 SO 4 + 0.05% NaN3 in 0.1mol/L Phosphate buffer, pH 6.7 at a flow rate of 0.5–1 ml/min.

    Techniques: Plasmid Preparation, Expressing, Amplification, Sequencing, Ligation, Enzyme-linked Immunosorbent Assay, Transfection, Clone Assay, Purification, Molecular Weight, SDS Page