confocal raman microscope  (JASCO Inc)


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    JASCO Inc confocal raman microscope
    Confocal Raman Microscope, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    confocal raman microscope  (JASCO Inc)


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    JASCO Inc confocal raman microscope
    Confocal Raman Microscope, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    JASCO Inc confocal raman microscope
    Confocal Raman Microscope, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    confocal raman microscope  (JASCO Inc)


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    JASCO Inc confocal raman microscope
    Confocal Raman Microscope, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    dxr confocal raman microscope  (JASCO Inc)


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    JASCO Inc dxr confocal raman microscope
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    confocal raman microscopy analysis  (JASCO Inc)


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    JASCO Inc confocal raman microscopy analysis
    Spatiotemporal metabolic imaging and quantification of linolenic acid and C18 fatty acids using LA- d 6 in A. thaliana roots under drought stress and water recovery. (a) Real-time SRS imaging at 2110 cm –1 of LA- d 6 localization in 1-week-old A. thaliana postdrought treatment (2 days) and recovered in water (min). The dotted line highlights the root hair boundary. The width of each image is 25 μm. (b, c) Average spectra <t>from</t> <t>confocal</t> <t>Raman</t> mapping of A. thaliana seedlings incubated with LA- d 6 without drought treatment and subsequent incubation in H 2 O and its inset (c) showing the Raman region between 1900 and 2200 cm –1 . (d) Normalized peak areas for the 2000–2200 cm –1 region for individual spectra taken from confocal Raman mapping. (e, f) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 with drought treatment for 1 and 2 days and subsequent recovery in dH 2 O and the inset (f) showing the Raman region between 1900 and 2200 cm –1 . (g) Normalized peak areas for the 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping. (h) Normalized peak area for C18 fatty acids as measured by GC–MS from fatty acids isolated from seedlings after drought and subsequent recovery. (i) Double bond index analysis to estimate the degree of unsaturation in the lipids calculated by the abundance from (h). DBI = 1 × % monosaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (j) Deuterium peak areas from LA- d 6 in a matrix of palmitic acid/plant lysate with varying probe solution ratios. (k) Relative Raman peak area from (i) plotted against the relative probe ratio. (l) Relative unsaturation levels estimated from normalized peak areas in (g) relative to the peak heights observed at 0 h recovery. (m) Linear relationship observed between relative unsaturation levels measured by Raman microscopy in (l) with DBI (i) and C18:3 quantification (f) as determined by GC–MS.
    Confocal Raman Microscopy Analysis, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    1) Product Images from "Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants"

    Article Title: Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

    Journal: JACS Au

    doi: 10.1021/jacsau.3c00041

    Spatiotemporal metabolic imaging and quantification of linolenic acid and C18 fatty acids using LA- d 6 in A. thaliana roots under drought stress and water recovery. (a) Real-time SRS imaging at 2110 cm –1 of LA- d 6 localization in 1-week-old A. thaliana postdrought treatment (2 days) and recovered in water (min). The dotted line highlights the root hair boundary. The width of each image is 25 μm. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 without drought treatment and subsequent incubation in H 2 O and its inset (c) showing the Raman region between 1900 and 2200 cm –1 . (d) Normalized peak areas for the 2000–2200 cm –1 region for individual spectra taken from confocal Raman mapping. (e, f) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 with drought treatment for 1 and 2 days and subsequent recovery in dH 2 O and the inset (f) showing the Raman region between 1900 and 2200 cm –1 . (g) Normalized peak areas for the 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping. (h) Normalized peak area for C18 fatty acids as measured by GC–MS from fatty acids isolated from seedlings after drought and subsequent recovery. (i) Double bond index analysis to estimate the degree of unsaturation in the lipids calculated by the abundance from (h). DBI = 1 × % monosaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (j) Deuterium peak areas from LA- d 6 in a matrix of palmitic acid/plant lysate with varying probe solution ratios. (k) Relative Raman peak area from (i) plotted against the relative probe ratio. (l) Relative unsaturation levels estimated from normalized peak areas in (g) relative to the peak heights observed at 0 h recovery. (m) Linear relationship observed between relative unsaturation levels measured by Raman microscopy in (l) with DBI (i) and C18:3 quantification (f) as determined by GC–MS.
    Figure Legend Snippet: Spatiotemporal metabolic imaging and quantification of linolenic acid and C18 fatty acids using LA- d 6 in A. thaliana roots under drought stress and water recovery. (a) Real-time SRS imaging at 2110 cm –1 of LA- d 6 localization in 1-week-old A. thaliana postdrought treatment (2 days) and recovered in water (min). The dotted line highlights the root hair boundary. The width of each image is 25 μm. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 without drought treatment and subsequent incubation in H 2 O and its inset (c) showing the Raman region between 1900 and 2200 cm –1 . (d) Normalized peak areas for the 2000–2200 cm –1 region for individual spectra taken from confocal Raman mapping. (e, f) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 with drought treatment for 1 and 2 days and subsequent recovery in dH 2 O and the inset (f) showing the Raman region between 1900 and 2200 cm –1 . (g) Normalized peak areas for the 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping. (h) Normalized peak area for C18 fatty acids as measured by GC–MS from fatty acids isolated from seedlings after drought and subsequent recovery. (i) Double bond index analysis to estimate the degree of unsaturation in the lipids calculated by the abundance from (h). DBI = 1 × % monosaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (j) Deuterium peak areas from LA- d 6 in a matrix of palmitic acid/plant lysate with varying probe solution ratios. (k) Relative Raman peak area from (i) plotted against the relative probe ratio. (l) Relative unsaturation levels estimated from normalized peak areas in (g) relative to the peak heights observed at 0 h recovery. (m) Linear relationship observed between relative unsaturation levels measured by Raman microscopy in (l) with DBI (i) and C18:3 quantification (f) as determined by GC–MS.

    Techniques Used: Imaging, Incubation, Gas Chromatography-Mass Spectrometry, Isolation, Microscopy

    Spatiotemporal metabolic imaging of C18 fatty acids and quantification using 17-ODYA in A. thaliana roots under heat stress. (a) In situ confocal Raman mapping of A. thaliana seedlings under heat stress at 35 °C from 0 to 8 h showing the relative intensity of the alkyne peak from 17-ODYA at 2120 cm –1 . The confocal light image (top), the relative alkyne peak areas at approx. 2000–2200 cm –1 (middle), and the peak height at 1430 cm –1 corresponding to C–H stretching as a control for the overall fatty acid content. The scale bars represent 2.5 μm, and the color bars represent the scaled Raman intensities for each representative image for the respective time course. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings grown with 17-ODYA under heat stress at 35 °C from 0 to 8 h and its inset (c) at 1900–2400 cm –1 . (d) Normalized peak areas for 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping (min. n = 30). (e) Quantification of normalized peak area for 18C fatty acids as measured by GC–MS from fatty acids isolated from seedlings before and after heat stress. (f) DBI analysis to estimate the degree of unsaturation in C16 and C18 lipids calculated from (e) DBI = 1 × % monounsaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (g) Alkyne peak areas from 17-ODYA in a matrix of 2% stearic acid/plant lysate solution with varying probe-to-matrix ratios. (h) Relative Raman peak area from (g) plotted against the relative probe ratio. (i) Relative unsaturation levels estimated from the normalized peak areas in (d) relative to the peak heights observed at 0 h recovery. (j) Linear relationship observed between DBI and C18:3 unsaturated fatty acid levels and the corresponding probe signal observed in the 17-ODYA Raman signal.
    Figure Legend Snippet: Spatiotemporal metabolic imaging of C18 fatty acids and quantification using 17-ODYA in A. thaliana roots under heat stress. (a) In situ confocal Raman mapping of A. thaliana seedlings under heat stress at 35 °C from 0 to 8 h showing the relative intensity of the alkyne peak from 17-ODYA at 2120 cm –1 . The confocal light image (top), the relative alkyne peak areas at approx. 2000–2200 cm –1 (middle), and the peak height at 1430 cm –1 corresponding to C–H stretching as a control for the overall fatty acid content. The scale bars represent 2.5 μm, and the color bars represent the scaled Raman intensities for each representative image for the respective time course. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings grown with 17-ODYA under heat stress at 35 °C from 0 to 8 h and its inset (c) at 1900–2400 cm –1 . (d) Normalized peak areas for 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping (min. n = 30). (e) Quantification of normalized peak area for 18C fatty acids as measured by GC–MS from fatty acids isolated from seedlings before and after heat stress. (f) DBI analysis to estimate the degree of unsaturation in C16 and C18 lipids calculated from (e) DBI = 1 × % monounsaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (g) Alkyne peak areas from 17-ODYA in a matrix of 2% stearic acid/plant lysate solution with varying probe-to-matrix ratios. (h) Relative Raman peak area from (g) plotted against the relative probe ratio. (i) Relative unsaturation levels estimated from the normalized peak areas in (d) relative to the peak heights observed at 0 h recovery. (j) Linear relationship observed between DBI and C18:3 unsaturated fatty acid levels and the corresponding probe signal observed in the 17-ODYA Raman signal.

    Techniques Used: Imaging, In Situ, Gas Chromatography-Mass Spectrometry, Isolation

    confocal raman microscope  (JASCO Inc)


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    JASCO Inc confocal raman microscope
    Confocal Raman Microscope, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    JASCO Inc confocal raman microscope
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    confocal micro raman spectrometer nrs 3100  (JASCO Inc)


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    JASCO Inc confocal micro raman spectrometer nrs 3100
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    JASCO Inc confocal raman microscope
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    JASCO Inc confocal raman microscope
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    JASCO Inc confocal raman microscopy analysis
    Spatiotemporal metabolic imaging and quantification of linolenic acid and C18 fatty acids using LA- d 6 in A. thaliana roots under drought stress and water recovery. (a) Real-time SRS imaging at 2110 cm –1 of LA- d 6 localization in 1-week-old A. thaliana postdrought treatment (2 days) and recovered in water (min). The dotted line highlights the root hair boundary. The width of each image is 25 μm. (b, c) Average spectra <t>from</t> <t>confocal</t> <t>Raman</t> mapping of A. thaliana seedlings incubated with LA- d 6 without drought treatment and subsequent incubation in H 2 O and its inset (c) showing the Raman region between 1900 and 2200 cm –1 . (d) Normalized peak areas for the 2000–2200 cm –1 region for individual spectra taken from confocal Raman mapping. (e, f) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 with drought treatment for 1 and 2 days and subsequent recovery in dH 2 O and the inset (f) showing the Raman region between 1900 and 2200 cm –1 . (g) Normalized peak areas for the 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping. (h) Normalized peak area for C18 fatty acids as measured by GC–MS from fatty acids isolated from seedlings after drought and subsequent recovery. (i) Double bond index analysis to estimate the degree of unsaturation in the lipids calculated by the abundance from (h). DBI = 1 × % monosaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (j) Deuterium peak areas from LA- d 6 in a matrix of palmitic acid/plant lysate with varying probe solution ratios. (k) Relative Raman peak area from (i) plotted against the relative probe ratio. (l) Relative unsaturation levels estimated from normalized peak areas in (g) relative to the peak heights observed at 0 h recovery. (m) Linear relationship observed between relative unsaturation levels measured by Raman microscopy in (l) with DBI (i) and C18:3 quantification (f) as determined by GC–MS.
    Confocal Raman Microscopy Analysis, supplied by JASCO Inc, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    JASCO Inc confocal micro raman spectrometer nrs 3100
    Spatiotemporal metabolic imaging and quantification of linolenic acid and C18 fatty acids using LA- d 6 in A. thaliana roots under drought stress and water recovery. (a) Real-time SRS imaging at 2110 cm –1 of LA- d 6 localization in 1-week-old A. thaliana postdrought treatment (2 days) and recovered in water (min). The dotted line highlights the root hair boundary. The width of each image is 25 μm. (b, c) Average spectra <t>from</t> <t>confocal</t> <t>Raman</t> mapping of A. thaliana seedlings incubated with LA- d 6 without drought treatment and subsequent incubation in H 2 O and its inset (c) showing the Raman region between 1900 and 2200 cm –1 . (d) Normalized peak areas for the 2000–2200 cm –1 region for individual spectra taken from confocal Raman mapping. (e, f) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 with drought treatment for 1 and 2 days and subsequent recovery in dH 2 O and the inset (f) showing the Raman region between 1900 and 2200 cm –1 . (g) Normalized peak areas for the 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping. (h) Normalized peak area for C18 fatty acids as measured by GC–MS from fatty acids isolated from seedlings after drought and subsequent recovery. (i) Double bond index analysis to estimate the degree of unsaturation in the lipids calculated by the abundance from (h). DBI = 1 × % monosaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (j) Deuterium peak areas from LA- d 6 in a matrix of palmitic acid/plant lysate with varying probe solution ratios. (k) Relative Raman peak area from (i) plotted against the relative probe ratio. (l) Relative unsaturation levels estimated from normalized peak areas in (g) relative to the peak heights observed at 0 h recovery. (m) Linear relationship observed between relative unsaturation levels measured by Raman microscopy in (l) with DBI (i) and C18:3 quantification (f) as determined by GC–MS.
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    Spatiotemporal metabolic imaging and quantification of linolenic acid and C18 fatty acids using LA- d 6 in A. thaliana roots under drought stress and water recovery. (a) Real-time SRS imaging at 2110 cm –1 of LA- d 6 localization in 1-week-old A. thaliana postdrought treatment (2 days) and recovered in water (min). The dotted line highlights the root hair boundary. The width of each image is 25 μm. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 without drought treatment and subsequent incubation in H 2 O and its inset (c) showing the Raman region between 1900 and 2200 cm –1 . (d) Normalized peak areas for the 2000–2200 cm –1 region for individual spectra taken from confocal Raman mapping. (e, f) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 with drought treatment for 1 and 2 days and subsequent recovery in dH 2 O and the inset (f) showing the Raman region between 1900 and 2200 cm –1 . (g) Normalized peak areas for the 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping. (h) Normalized peak area for C18 fatty acids as measured by GC–MS from fatty acids isolated from seedlings after drought and subsequent recovery. (i) Double bond index analysis to estimate the degree of unsaturation in the lipids calculated by the abundance from (h). DBI = 1 × % monosaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (j) Deuterium peak areas from LA- d 6 in a matrix of palmitic acid/plant lysate with varying probe solution ratios. (k) Relative Raman peak area from (i) plotted against the relative probe ratio. (l) Relative unsaturation levels estimated from normalized peak areas in (g) relative to the peak heights observed at 0 h recovery. (m) Linear relationship observed between relative unsaturation levels measured by Raman microscopy in (l) with DBI (i) and C18:3 quantification (f) as determined by GC–MS.

    Journal: JACS Au

    Article Title: Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

    doi: 10.1021/jacsau.3c00041

    Figure Lengend Snippet: Spatiotemporal metabolic imaging and quantification of linolenic acid and C18 fatty acids using LA- d 6 in A. thaliana roots under drought stress and water recovery. (a) Real-time SRS imaging at 2110 cm –1 of LA- d 6 localization in 1-week-old A. thaliana postdrought treatment (2 days) and recovered in water (min). The dotted line highlights the root hair boundary. The width of each image is 25 μm. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 without drought treatment and subsequent incubation in H 2 O and its inset (c) showing the Raman region between 1900 and 2200 cm –1 . (d) Normalized peak areas for the 2000–2200 cm –1 region for individual spectra taken from confocal Raman mapping. (e, f) Average spectra from confocal Raman mapping of A. thaliana seedlings incubated with LA- d 6 with drought treatment for 1 and 2 days and subsequent recovery in dH 2 O and the inset (f) showing the Raman region between 1900 and 2200 cm –1 . (g) Normalized peak areas for the 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping. (h) Normalized peak area for C18 fatty acids as measured by GC–MS from fatty acids isolated from seedlings after drought and subsequent recovery. (i) Double bond index analysis to estimate the degree of unsaturation in the lipids calculated by the abundance from (h). DBI = 1 × % monosaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (j) Deuterium peak areas from LA- d 6 in a matrix of palmitic acid/plant lysate with varying probe solution ratios. (k) Relative Raman peak area from (i) plotted against the relative probe ratio. (l) Relative unsaturation levels estimated from normalized peak areas in (g) relative to the peak heights observed at 0 h recovery. (m) Linear relationship observed between relative unsaturation levels measured by Raman microscopy in (l) with DBI (i) and C18:3 quantification (f) as determined by GC–MS.

    Article Snippet: Confocal Raman microscopy analysis was performed using a JASCO NRS-4500 Raman Microscope (JASCO, Japan).

    Techniques: Imaging, Incubation, Gas Chromatography-Mass Spectrometry, Isolation, Microscopy

    Spatiotemporal metabolic imaging of C18 fatty acids and quantification using 17-ODYA in A. thaliana roots under heat stress. (a) In situ confocal Raman mapping of A. thaliana seedlings under heat stress at 35 °C from 0 to 8 h showing the relative intensity of the alkyne peak from 17-ODYA at 2120 cm –1 . The confocal light image (top), the relative alkyne peak areas at approx. 2000–2200 cm –1 (middle), and the peak height at 1430 cm –1 corresponding to C–H stretching as a control for the overall fatty acid content. The scale bars represent 2.5 μm, and the color bars represent the scaled Raman intensities for each representative image for the respective time course. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings grown with 17-ODYA under heat stress at 35 °C from 0 to 8 h and its inset (c) at 1900–2400 cm –1 . (d) Normalized peak areas for 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping (min. n = 30). (e) Quantification of normalized peak area for 18C fatty acids as measured by GC–MS from fatty acids isolated from seedlings before and after heat stress. (f) DBI analysis to estimate the degree of unsaturation in C16 and C18 lipids calculated from (e) DBI = 1 × % monounsaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (g) Alkyne peak areas from 17-ODYA in a matrix of 2% stearic acid/plant lysate solution with varying probe-to-matrix ratios. (h) Relative Raman peak area from (g) plotted against the relative probe ratio. (i) Relative unsaturation levels estimated from the normalized peak areas in (d) relative to the peak heights observed at 0 h recovery. (j) Linear relationship observed between DBI and C18:3 unsaturated fatty acid levels and the corresponding probe signal observed in the 17-ODYA Raman signal.

    Journal: JACS Au

    Article Title: Deuterium- and Alkyne-Based Bioorthogonal Raman Probes for In Situ Quantitative Metabolic Imaging of Lipids within Plants

    doi: 10.1021/jacsau.3c00041

    Figure Lengend Snippet: Spatiotemporal metabolic imaging of C18 fatty acids and quantification using 17-ODYA in A. thaliana roots under heat stress. (a) In situ confocal Raman mapping of A. thaliana seedlings under heat stress at 35 °C from 0 to 8 h showing the relative intensity of the alkyne peak from 17-ODYA at 2120 cm –1 . The confocal light image (top), the relative alkyne peak areas at approx. 2000–2200 cm –1 (middle), and the peak height at 1430 cm –1 corresponding to C–H stretching as a control for the overall fatty acid content. The scale bars represent 2.5 μm, and the color bars represent the scaled Raman intensities for each representative image for the respective time course. (b, c) Average spectra from confocal Raman mapping of A. thaliana seedlings grown with 17-ODYA under heat stress at 35 °C from 0 to 8 h and its inset (c) at 1900–2400 cm –1 . (d) Normalized peak areas for 2000–2200 cm –1 for individual spectra taken from confocal Raman mapping (min. n = 30). (e) Quantification of normalized peak area for 18C fatty acids as measured by GC–MS from fatty acids isolated from seedlings before and after heat stress. (f) DBI analysis to estimate the degree of unsaturation in C16 and C18 lipids calculated from (e) DBI = 1 × % monounsaturated fatty acids + 2 × % diunsaturated fatty acids + 3 × % triunsaturated fatty acids. (g) Alkyne peak areas from 17-ODYA in a matrix of 2% stearic acid/plant lysate solution with varying probe-to-matrix ratios. (h) Relative Raman peak area from (g) plotted against the relative probe ratio. (i) Relative unsaturation levels estimated from the normalized peak areas in (d) relative to the peak heights observed at 0 h recovery. (j) Linear relationship observed between DBI and C18:3 unsaturated fatty acid levels and the corresponding probe signal observed in the 17-ODYA Raman signal.

    Article Snippet: Confocal Raman microscopy analysis was performed using a JASCO NRS-4500 Raman Microscope (JASCO, Japan).

    Techniques: Imaging, In Situ, Gas Chromatography-Mass Spectrometry, Isolation