jak inhibitor i  (Millipore)

 
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    Name:
    Aurora A Inhibitor I
    Description:

    Catalog Number:
    sml0882
    Price:
    None
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    Structured Review

    Millipore jak inhibitor i
    JAK1/2 is required for autophagy early after IFN-γ stimulation. A-C , RAW-tfLC3 was retreated with <t>JAK</t> <t>inhibitor</t> I or left untreated for 2 h, and then the cells were stimulated with 200 U/ml IFN-γ for 4 h. The cells were fixed and stained

    https://www.bioz.com/result/jak inhibitor i/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jak inhibitor i - by Bioz Stars, 2021-03
    97/100 stars

    Images

    1) Product Images from "IFN-? elicits macrophage autophagy via the p38 MAPK signaling pathway"

    Article Title: IFN-? elicits macrophage autophagy via the p38 MAPK signaling pathway

    Journal: Journal of Immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1102041

    JAK1/2 is required for autophagy early after IFN-γ stimulation. A-C , RAW-tfLC3 was retreated with JAK inhibitor I or left untreated for 2 h, and then the cells were stimulated with 200 U/ml IFN-γ for 4 h. The cells were fixed and stained
    Figure Legend Snippet: JAK1/2 is required for autophagy early after IFN-γ stimulation. A-C , RAW-tfLC3 was retreated with JAK inhibitor I or left untreated for 2 h, and then the cells were stimulated with 200 U/ml IFN-γ for 4 h. The cells were fixed and stained

    Techniques Used: Staining

    2) Product Images from "MPLW515L Is a Novel Somatic Activating Mutation in Myelofibrosis with Myeloid Metaplasia"

    Article Title: MPLW515L Is a Novel Somatic Activating Mutation in Myelofibrosis with Myeloid Metaplasia

    Journal: PLoS Medicine

    doi: 10.1371/journal.pmed.0030270

    32D MPLW515L Cells Are Sensitive to JAK Inhibitor I (A) Dose-dependent inhibition of growth of 32D MPLW515L and 32D MPLWT cells but not 32D FIP1L1-PDGFRA cells with increasing doses of JAK Inhibitor I. (B) Reduction in STAT3 phosphorylation in 32D MPLW515L cells but not 32D FIP1L1-PDGFRA cells with increasing doses of JAK Inhibitor I. Cells were incubated with varying drug concentrations for four hours and then collected for Western blot analysis.
    Figure Legend Snippet: 32D MPLW515L Cells Are Sensitive to JAK Inhibitor I (A) Dose-dependent inhibition of growth of 32D MPLW515L and 32D MPLWT cells but not 32D FIP1L1-PDGFRA cells with increasing doses of JAK Inhibitor I. (B) Reduction in STAT3 phosphorylation in 32D MPLW515L cells but not 32D FIP1L1-PDGFRA cells with increasing doses of JAK Inhibitor I. Cells were incubated with varying drug concentrations for four hours and then collected for Western blot analysis.

    Techniques Used: Inhibition, Incubation, Western Blot

    3) Product Images from "Extended JAK Activation and Delayed STAT1 Dephosphorylation Contribute to the Distinct Signaling Profile of CNS Neurons Exposed to Interferon-Gamma"

    Article Title: Extended JAK Activation and Delayed STAT1 Dephosphorylation Contribute to the Distinct Signaling Profile of CNS Neurons Exposed to Interferon-Gamma

    Journal: Journal of neuroimmunology

    doi: 10.1016/j.jneuroim.2012.06.006

    JAK inhibitor I effectively prevents IFN-γ-stimulated STAT1 activation in neurons
    Figure Legend Snippet: JAK inhibitor I effectively prevents IFN-γ-stimulated STAT1 activation in neurons

    Techniques Used: Activation Assay

    Related Articles

    Recombinant:

    Article Title: Arpc1b, a centrosomal protein, is both an activator and substrate of Aurora A
    Article Snippet: .. Anderson Cancer Center, Houston, TX). (His)6-tagged or GST-fused recombinant full-length human Aurora A was purchased from Millipore and Cell Signaling Technology, respectively. .. Antibody against T7-epitope (Novagen); actin, vinculin, γ-tubulin (Sigma-Aldrich); Aurora A, Rac1 (BD; Cell Signaling Technology); Pak1, Pak2, phopho-Histone H3 (Ser10), Aurora A, GST, and Arp3 (Cell Signaling Technology); Arpc1b (Imgenex; Santa Cruz Biotechnology, Inc.); Arpc2, Arp3 (Millipore); Arpc2 and Pak3 (Santa Cruz Biotechnology, Inc.); Phospho-Aurora A (Thr288) (Novus Biologicals); and Plk-1, phospho-plk1 (Thr210) (BioLegend) were used.

    TUNEL Assay:

    Article Title: Epicardial FSTL1 reconstitution regenerates the adult mammalian heart
    Article Snippet: Ratios of EdU+/ α-actinin+ nuclei and α-actinin+ nuclei were generated for the percentage of cardiomyocyte incorporated EdU in the chromosomal DNA. .. Similarly, cells (mCMsESC and NRVC) in 384-well plate format were fixed for 2 h in 4% PFA, washed in PBS, and were immunostained with pH3 antibody (Millipore 06-570, 1:200) for nuclei in mitosis, or aurora B (Millipore 04-1036, 1:200) for cytokinesis, or TUNEL (Roche) for cell death, and α-actinin antibody (Sigma, A7811, 1:500) for cardiomyocytes and DAPI (1:10,000) for nuclei. .. The same imaging and analysis were done for pH3 staining as the EdU assays, and the aurora B+ , α-actinin+ double positive cells were manually counted.

    Expressing:

    Article Title: A Translational Regulator, PUM2, Promotes Both Protein Stability and Kinase Activity of Aurora-A
    Article Snippet: .. Construction of expression vectors, small interference RNA (siRNA) and Site-directed mutagenesis Human PUM2 cDNA and Aurora-A were subcloned into pFLAG-CMV-2 vector (Sigma) and pcDNA3.0 (Invitrogen) to produce FLAG-tagged and HA-tagged plasmids. .. To generate GST fusion protein, PUM2 cDNA were subcloned to pGEX4T-2 vector (Amersham Pharmacia Biotech).

    Mutagenesis:

    Article Title: A Translational Regulator, PUM2, Promotes Both Protein Stability and Kinase Activity of Aurora-A
    Article Snippet: .. Construction of expression vectors, small interference RNA (siRNA) and Site-directed mutagenesis Human PUM2 cDNA and Aurora-A were subcloned into pFLAG-CMV-2 vector (Sigma) and pcDNA3.0 (Invitrogen) to produce FLAG-tagged and HA-tagged plasmids. .. To generate GST fusion protein, PUM2 cDNA were subcloned to pGEX4T-2 vector (Amersham Pharmacia Biotech).

    Plasmid Preparation:

    Article Title: A Translational Regulator, PUM2, Promotes Both Protein Stability and Kinase Activity of Aurora-A
    Article Snippet: .. Construction of expression vectors, small interference RNA (siRNA) and Site-directed mutagenesis Human PUM2 cDNA and Aurora-A were subcloned into pFLAG-CMV-2 vector (Sigma) and pcDNA3.0 (Invitrogen) to produce FLAG-tagged and HA-tagged plasmids. .. To generate GST fusion protein, PUM2 cDNA were subcloned to pGEX4T-2 vector (Amersham Pharmacia Biotech).

    Activity Assay:

    Article Title: Development of ortho-Chlorophenyl Substituted Pyrimidines as Exceptionally Potent Aurora Kinase Inhibitors
    Article Snippet: .. Aurora A activity was determined by measuring autophosphorylation of Aurora A on Thr288 in cells that were synchronized by treatment with nocodazole (100 ng/ml) (Sigma) for 20 h prior to the treatment with Aurora inhibitors, whereas Aurora B activity was determined by measuring phosphorylation of histone H3 on Ser10 (pHisH3-Ser10). .. Cells were then treated with the compounds (0-10 μM); DMSO was used as a negative control and MLN8237 (Alisertib) (0.3 μM) or VX-680 (Tozasertib) (0.5 μM) (Selleck Chemical LLC) were used as a positive control.

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  • 93
    Millipore incb 18424 jak i calbiochem
    Integrated biochemical characterization with viability/survival assays in hypodiploid ALL: A) Cytobank heatmap representing a signaling profiling panel of phospho- and total protein levels (columns) in individually xenografted leukemias from LH and NH subtypes (rows). Levels of phospho- and total proteins were normalized to healthy donor control cells (HM ctrl), yellow indicating increased levels and blue indicating decreased levels. (B and C) NALM-16 cells were cultured for 24h in the presence of increasing concentrations of inhibitors (PP2, <t>INCB-18424,</t> GDC-0941, PP-242, PD-0325901, ABT-263) and effects were measured using (B) cell proliferation and (C) apoptosis levels using ATP-luminescence (Cell Titer Glo) or Caspase 3/7 activation assays, respectively.
    Incb 18424 Jak I Calbiochem, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incb 18424 jak i calbiochem/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    incb 18424 jak i calbiochem - by Bioz Stars, 2021-03
    93/100 stars
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    97
    Millipore jak inhibitor
    Role of STAT3 signalling in dorsal horn astrocyte proliferation after peripheral nerve injury. ( A ) Schematic time-line for intrathecal administration and fixation. <t>AG490</t> and <t>JAK</t> Inhibitor I, respective inhibitors of the STAT3 activator JAK, were administered
    Jak Inhibitor, supplied by Millipore, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jak inhibitor/product/Millipore
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jak inhibitor - by Bioz Stars, 2021-03
    97/100 stars
      Buy from Supplier

    94
    Millipore jak inhibitor i
    LPS, IL-6, and IFN-γ increase apelin promoter activity; blockade of IL-6- and IFN-γ-stimulated apelin promoter activity by a Jak/Stat inhibitor. A and B : LPS treatment increases rat apelin promoter activity 2- to 3-fold in macrophages but not in colon (HCT116) cells. The 5′-flanking region (−3000/−1; −2500/−1; −2000/−1; −1500/−1; −1000/−1; −506/−1; −407/−1; −302/−1; −207/−1; −106/−1 bp) of the rat apelin gene fused to a luciferase reporter gene was transfected into macrophage or colon cells and treated with LPS (1 μg/ml). Luciferase activities were measured 18 h after start of LPS treatment. C : IL-6- and IFN-γ-stimulated apelin promoter activity is mediated by the Jak/Stat pathway. The 5′-flanking region (−207/−1; −106/−1 bp) of the rat apelin gene fused to a luciferase reporter gene was transfected into mouse macrophages and treated with IL-6 (100 ng/ml) or IFN-γ (100 ng/ml) alone or in combination with a Jak <t>inhibitor</t> I (10 μM). IL-6 and IFN-γ treatments increased apelin promoter activity; the stimulatory effects of IL-6 and IFN-γ on apelin promoter activity were blocked by pretreatment with a Jak inhibitor I.
    Jak Inhibitor I, supplied by Millipore, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/jak inhibitor i/product/Millipore
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    jak inhibitor i - by Bioz Stars, 2021-03
    94/100 stars
      Buy from Supplier

    Image Search Results


    Integrated biochemical characterization with viability/survival assays in hypodiploid ALL: A) Cytobank heatmap representing a signaling profiling panel of phospho- and total protein levels (columns) in individually xenografted leukemias from LH and NH subtypes (rows). Levels of phospho- and total proteins were normalized to healthy donor control cells (HM ctrl), yellow indicating increased levels and blue indicating decreased levels. (B and C) NALM-16 cells were cultured for 24h in the presence of increasing concentrations of inhibitors (PP2, INCB-18424, GDC-0941, PP-242, PD-0325901, ABT-263) and effects were measured using (B) cell proliferation and (C) apoptosis levels using ATP-luminescence (Cell Titer Glo) or Caspase 3/7 activation assays, respectively.

    Journal: Cancer research

    Article Title: BCL-2 IS A THERAPEUTIC TARGET FOR HYPODIPLOID B-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA

    doi: 10.1158/0008-5472.CAN-18-0236

    Figure Lengend Snippet: Integrated biochemical characterization with viability/survival assays in hypodiploid ALL: A) Cytobank heatmap representing a signaling profiling panel of phospho- and total protein levels (columns) in individually xenografted leukemias from LH and NH subtypes (rows). Levels of phospho- and total proteins were normalized to healthy donor control cells (HM ctrl), yellow indicating increased levels and blue indicating decreased levels. (B and C) NALM-16 cells were cultured for 24h in the presence of increasing concentrations of inhibitors (PP2, INCB-18424, GDC-0941, PP-242, PD-0325901, ABT-263) and effects were measured using (B) cell proliferation and (C) apoptosis levels using ATP-luminescence (Cell Titer Glo) or Caspase 3/7 activation assays, respectively.

    Article Snippet: Inhibitors:ABT-199 kindly provided by ABBVIE, ABT-737 (Selleck Chemicals, S1002), PP2 (Calbiochem, 529576), INCB-18424 (JAK-I) Calbiochem (420099), GDC0941(Calbiochem, 509226), PP242 (Calbiochem, 475988), PD0325901 (Calbiochem, 444968), PI-90, PI-103, p110α, p110β, p110δ, (were kindly provided by prof. K. Shokat (UCSF)), AKT VIII (Calbiochem, 124018), Dexamethasone (Calbiochem, 265005), Etoposide (Calbiochem, 341205), Cisplatin (Calbiochem, 232120),.

    Techniques: Cell Culture, Activation Assay

    Role of STAT3 signalling in dorsal horn astrocyte proliferation after peripheral nerve injury. ( A ) Schematic time-line for intrathecal administration and fixation. AG490 and JAK Inhibitor I, respective inhibitors of the STAT3 activator JAK, were administered

    Journal: Brain

    Article Title: JAK-STAT3 pathway regulates spinal astrocyte proliferation and neuropathic pain maintenance in rats

    doi: 10.1093/brain/awr025

    Figure Lengend Snippet: Role of STAT3 signalling in dorsal horn astrocyte proliferation after peripheral nerve injury. ( A ) Schematic time-line for intrathecal administration and fixation. AG490 and JAK Inhibitor I, respective inhibitors of the STAT3 activator JAK, were administered

    Article Snippet: Rats were injected with either AG490 (3 and 10 nmol/10 μl, Calbiochem, CA, USA), JAK Inhibitor I, 2-(1,1-Dimethylethyl)-9-fluoro-3,6-dihydro-7 H-benz[h]-imidaz[4,5-f]isoquinolin-7-one, 2.5 nmol/10 μl, Calbiochem) or flavopiridol (5 nmol/10 μl, Santa Cruz Biotechnology, CA, USA) through the catheter once at 09:00 (AG490 and JAK Inhibitor I) or twice (once at 09:00 and once at 19:00; flavopiridol) a day from Days 3 to 5 (for immunohistochemical experiments of proliferating cells) or to Day 7 (for behavioural experiments) ( A, A and B).

    Techniques:

    LPS, IL-6, and IFN-γ increase apelin promoter activity; blockade of IL-6- and IFN-γ-stimulated apelin promoter activity by a Jak/Stat inhibitor. A and B : LPS treatment increases rat apelin promoter activity 2- to 3-fold in macrophages but not in colon (HCT116) cells. The 5′-flanking region (−3000/−1; −2500/−1; −2000/−1; −1500/−1; −1000/−1; −506/−1; −407/−1; −302/−1; −207/−1; −106/−1 bp) of the rat apelin gene fused to a luciferase reporter gene was transfected into macrophage or colon cells and treated with LPS (1 μg/ml). Luciferase activities were measured 18 h after start of LPS treatment. C : IL-6- and IFN-γ-stimulated apelin promoter activity is mediated by the Jak/Stat pathway. The 5′-flanking region (−207/−1; −106/−1 bp) of the rat apelin gene fused to a luciferase reporter gene was transfected into mouse macrophages and treated with IL-6 (100 ng/ml) or IFN-γ (100 ng/ml) alone or in combination with a Jak inhibitor I (10 μM). IL-6 and IFN-γ treatments increased apelin promoter activity; the stimulatory effects of IL-6 and IFN-γ on apelin promoter activity were blocked by pretreatment with a Jak inhibitor I.

    Journal: American Journal of Physiology - Gastrointestinal and Liver Physiology

    Article Title: Involvement of a Stat3 binding site in inflammation-induced enteric apelin expression

    doi: 10.1152/ajpgi.90493.2008

    Figure Lengend Snippet: LPS, IL-6, and IFN-γ increase apelin promoter activity; blockade of IL-6- and IFN-γ-stimulated apelin promoter activity by a Jak/Stat inhibitor. A and B : LPS treatment increases rat apelin promoter activity 2- to 3-fold in macrophages but not in colon (HCT116) cells. The 5′-flanking region (−3000/−1; −2500/−1; −2000/−1; −1500/−1; −1000/−1; −506/−1; −407/−1; −302/−1; −207/−1; −106/−1 bp) of the rat apelin gene fused to a luciferase reporter gene was transfected into macrophage or colon cells and treated with LPS (1 μg/ml). Luciferase activities were measured 18 h after start of LPS treatment. C : IL-6- and IFN-γ-stimulated apelin promoter activity is mediated by the Jak/Stat pathway. The 5′-flanking region (−207/−1; −106/−1 bp) of the rat apelin gene fused to a luciferase reporter gene was transfected into mouse macrophages and treated with IL-6 (100 ng/ml) or IFN-γ (100 ng/ml) alone or in combination with a Jak inhibitor I (10 μM). IL-6 and IFN-γ treatments increased apelin promoter activity; the stimulatory effects of IL-6 and IFN-γ on apelin promoter activity were blocked by pretreatment with a Jak inhibitor I.

    Article Snippet: Primary cultured ileal cells were pretreated with a Jak Inhibitor I (EMD Chemicals, Gibbstown, NJ; 10 μM) before exposure to IL-6 (100 ng/ml, 24 h).

    Techniques: Activity Assay, Luciferase, Transfection

    Janus kinase signaling is required for IFN-γ-mediated viral clearance. (A) MV-infected brain explants were left untreated or treated with 10 μM Jak Inhibitor I (Jak Inhib), IFN-γ, or both IFN-γ and 10 μM Jak Inhibitor

    Journal: Journal of Virology

    Article Title: T Cell-, Interleukin-12-, and Gamma Interferon-Driven Viral Clearance in Measles Virus-Infected Brain Tissue ▿

    doi: 10.1128/JVI.01496-10

    Figure Lengend Snippet: Janus kinase signaling is required for IFN-γ-mediated viral clearance. (A) MV-infected brain explants were left untreated or treated with 10 μM Jak Inhibitor I (Jak Inhib), IFN-γ, or both IFN-γ and 10 μM Jak Inhibitor

    Article Snippet: Jak Inhibitor I (Calbiochem, La Jolla, CA) was dissolved in dimethyl sulfoxide to produce a 1 mM stock and then diluted in brain explant medium to produce working solutions of 0.1 μM, 1.0 μM, and 10 μM.

    Techniques: Infection, Inhibition