j lat a72  (ATCC)


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    ATCC j lat a72
    Requirement of RelB for the activation of latent HIV-1 in J-Lat <t>A72</t> cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized
    J Lat A72, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/j lat a72/product/ATCC
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    j lat a72 - by Bioz Stars, 2024-05
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    1) Product Images from "Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression"

    Article Title: Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression

    Journal: Retrovirology

    doi: 10.1186/s12977-018-0447-9

    Requirement of RelB for the activation of latent HIV-1 in J-Lat A72 cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized
    Figure Legend Snippet: Requirement of RelB for the activation of latent HIV-1 in J-Lat A72 cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized

    Techniques Used: Activation Assay, Western Blot, Knock-Out, Transduction, Expressing, Infection, Flow Cytometry, Software

    j lat a72 crispri  (ATCC)


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    ATCC j lat a72 crispri
    J Lat A72 Crispri, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    j lat a72  (ATCC)


    Bioz Verified Symbol ATCC is a verified supplier
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    ATCC j lat a72
    Requirement of RelB for the activation of latent HIV-1 in J-Lat <t>A72</t> cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized
    J Lat A72, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/j lat a72/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    j lat a72 - by Bioz Stars, 2024-05
    93/100 stars

    Images

    1) Product Images from "Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression"

    Article Title: Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression

    Journal: Retrovirology

    doi: 10.1186/s12977-018-0447-9

    Requirement of RelB for the activation of latent HIV-1 in J-Lat A72 cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized
    Figure Legend Snippet: Requirement of RelB for the activation of latent HIV-1 in J-Lat A72 cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized

    Techniques Used: Activation Assay, Western Blot, Knock-Out, Transduction, Expressing, Infection, Flow Cytometry, Software

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    ATCC j lat a72
    Requirement of RelB for the activation of latent HIV-1 in J-Lat <t>A72</t> cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized
    J Lat A72, supplied by ATCC, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/j lat a72/product/ATCC
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    j lat a72 - by Bioz Stars, 2024-05
    93/100 stars
      Buy from Supplier

    86
    ATCC j lat a72 crispri
    Requirement of RelB for the activation of latent HIV-1 in J-Lat <t>A72</t> cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized
    J Lat A72 Crispri, supplied by ATCC, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/j lat a72 crispri/product/ATCC
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    j lat a72 crispri - by Bioz Stars, 2024-05
    86/100 stars
      Buy from Supplier

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    Requirement of RelB for the activation of latent HIV-1 in J-Lat A72 cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized

    Journal: Retrovirology

    Article Title: Cellular RelB interacts with the transactivator Tat and enhance HIV-1 expression

    doi: 10.1186/s12977-018-0447-9

    Figure Lengend Snippet: Requirement of RelB for the activation of latent HIV-1 in J-Lat A72 cells. a Illustration of the J-Lat A72 latency model. b Western blots to examine the overexpressed RelB or RelB knockout in J-Lat A72 cell lines. c RelB enhanced Tat-mediated activation of latent HIV-1 in J-Lat A72 cells. J-Lat A72 cells (2 × 10 6 ) were transduced with VSV-G pseudotyped lentiviral VLPs expressing Tat. Twenty-four hours post infection, cells were treated with 10 nM PMA or DMSO for 24 h, then subjected to flow cytometry to detect GFP expressing cells using a FACSCalibur flow cytometer. Data were analyzed using the FlowJo software. d Analysis of HIV-1 LTR transcriptional activation by flow cytometry. Results from three independent experiments were summarized

    Article Snippet: Jurkat (ATCC, TIB-152), J-Lat A72 (NIH, CAT# 9856) [ , ], and peripheral blood mononuclear cells (PBMCs) were maintained in RPMI 1640 (Corning) with 10% FBS and 2.4 mM l -glutamine (Gibco).

    Techniques: Activation Assay, Western Blot, Knock-Out, Transduction, Expressing, Infection, Flow Cytometry, Software