Structured Review

IBEX Technologies iu heparinase iii
En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep <t>III,</t> and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after <t>heparinase</t> III treatment and recovered after HS addition. * P
Iu Heparinase Iii, supplied by IBEX Technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/iu heparinase iii/product/IBEX Technologies
Average 90 stars, based on 1 article reviews
Price from $9.99 to $1999.99
iu heparinase iii - by Bioz Stars, 2020-11
90/100 stars

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penicillin-streptomycin
glycocalyx configuration

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1) Product Images from "Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting"

Article Title: Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting

Journal: International Journal of Nanomedicine

doi: 10.2147/IJN.S106299

En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P
Figure Legend Snippet: En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P

Techniques Used: Binding Assay

Related Articles

Incubation:

Article Title: De Novo Sequencing of Complex Mixtures of Heparan Sulfate Oligosaccharides
Article Snippet: .. The solution was incubated at 37 °C for a total of 48 h, with an additional aliquot of 0.05 IU heparinase III added after 24 h incubation. .. The enzyme digestion was stopped by heating the solution at 100 °C for 10 min.

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    IBEX Technologies iu l heparinase iii
    Interstitial flow-induced ERK activation depends on both FAK and HSPGs. ( A ) Western blots showed that PF-228 and Hep reduced ERK phosphorylation; Cells in gels were pretreated with 10 µM of PF-228 or 6.7 <t>IU/L</t> of Hep for 2 h followed by exposure to flow for 0, 15, or 30 min. ( B ) Western blots showed that shFAK or shNDST1 significantly reduced flow-induced ERK activation. Flow was induced for 0, 15, or 30 min. The gel panels are representative images from <t>three</t> independent experiments. Fold change values are the ratios of p-ERK1 and p-ERK2 over the total ERK1 and total ERK2, and then normalized to no-flow control case, shown in the bar graph. * P
    Iu L Heparinase Iii, supplied by IBEX Technologies, used in various techniques. Bioz Stars score: 85/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iu l heparinase iii/product/IBEX Technologies
    Average 85 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    iu l heparinase iii - by Bioz Stars, 2020-11
    85/100 stars
      Buy from Supplier

    91
    IBEX Technologies heparitinase
    Interstitial flow-induced ERK activation depends on both FAK and HSPGs. ( A ) Western blots showed that PF-228 and Hep reduced ERK phosphorylation; Cells in gels were pretreated with 10 µM of PF-228 or 6.7 <t>IU/L</t> of Hep for 2 h followed by exposure to flow for 0, 15, or 30 min. ( B ) Western blots showed that shFAK or shNDST1 significantly reduced flow-induced ERK activation. Flow was induced for 0, 15, or 30 min. The gel panels are representative images from <t>three</t> independent experiments. Fold change values are the ratios of p-ERK1 and p-ERK2 over the total ERK1 and total ERK2, and then normalized to no-flow control case, shown in the bar graph. * P
    Heparitinase, supplied by IBEX Technologies, used in various techniques. Bioz Stars score: 91/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heparitinase/product/IBEX Technologies
    Average 91 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    heparitinase - by Bioz Stars, 2020-11
    91/100 stars
      Buy from Supplier

    Image Search Results


    Interstitial flow-induced ERK activation depends on both FAK and HSPGs. ( A ) Western blots showed that PF-228 and Hep reduced ERK phosphorylation; Cells in gels were pretreated with 10 µM of PF-228 or 6.7 IU/L of Hep for 2 h followed by exposure to flow for 0, 15, or 30 min. ( B ) Western blots showed that shFAK or shNDST1 significantly reduced flow-induced ERK activation. Flow was induced for 0, 15, or 30 min. The gel panels are representative images from three independent experiments. Fold change values are the ratios of p-ERK1 and p-ERK2 over the total ERK1 and total ERK2, and then normalized to no-flow control case, shown in the bar graph. * P

    Journal: PLoS ONE

    Article Title: Heparan Sulfate Proteoglycans Mediate Interstitial Flow Mechanotransduction Regulating MMP-13 Expression and Cell Motility via FAK-ERK in 3D Collagen

    doi: 10.1371/journal.pone.0015956

    Figure Lengend Snippet: Interstitial flow-induced ERK activation depends on both FAK and HSPGs. ( A ) Western blots showed that PF-228 and Hep reduced ERK phosphorylation; Cells in gels were pretreated with 10 µM of PF-228 or 6.7 IU/L of Hep for 2 h followed by exposure to flow for 0, 15, or 30 min. ( B ) Western blots showed that shFAK or shNDST1 significantly reduced flow-induced ERK activation. Flow was induced for 0, 15, or 30 min. The gel panels are representative images from three independent experiments. Fold change values are the ratios of p-ERK1 and p-ERK2 over the total ERK1 and total ERK2, and then normalized to no-flow control case, shown in the bar graph. * P

    Article Snippet: For FAK inhibition and HSPGs cleavage experiments, after 24 h of spreading, cells in gels were treated with either 10 µM PF-228 (Santa Cruz Biotechnology) or 6.7 IU/L heparinase III (IBEX Technologies, Montreal, Canada) in growth medium for 2 h and then exposed to flow.

    Techniques: Flow Cytometry, Activation Assay, Western Blot

    HSPGs mediate interstitial flow-induced activation of FAK Tyr925 and ERK. Cells in gels were pretreated with 6.7 IU/L of Hep for 2 h to remove cell surface HSPGs. Flow was induced for 0, 5, 10 or 15 min. Removal of HSPGs reduced FAK phosphorylation at Tyr397, but interstitial flow did not affect Tyr397 phosphorylation. Flow promoted Tyr925 and ERK phosphorylation, and cleavage of HSPGs blocked the flow effects. The gel panels are representative images from three independent experiments. Fold change values are the ratios of p-Tyr925 FAK over its internal control β-actin, and then normalized to no-flow control, shown in the bar graph. * P

    Journal: PLoS ONE

    Article Title: Heparan Sulfate Proteoglycans Mediate Interstitial Flow Mechanotransduction Regulating MMP-13 Expression and Cell Motility via FAK-ERK in 3D Collagen

    doi: 10.1371/journal.pone.0015956

    Figure Lengend Snippet: HSPGs mediate interstitial flow-induced activation of FAK Tyr925 and ERK. Cells in gels were pretreated with 6.7 IU/L of Hep for 2 h to remove cell surface HSPGs. Flow was induced for 0, 5, 10 or 15 min. Removal of HSPGs reduced FAK phosphorylation at Tyr397, but interstitial flow did not affect Tyr397 phosphorylation. Flow promoted Tyr925 and ERK phosphorylation, and cleavage of HSPGs blocked the flow effects. The gel panels are representative images from three independent experiments. Fold change values are the ratios of p-Tyr925 FAK over its internal control β-actin, and then normalized to no-flow control, shown in the bar graph. * P

    Article Snippet: For FAK inhibition and HSPGs cleavage experiments, after 24 h of spreading, cells in gels were treated with either 10 µM PF-228 (Santa Cruz Biotechnology) or 6.7 IU/L heparinase III (IBEX Technologies, Montreal, Canada) in growth medium for 2 h and then exposed to flow.

    Techniques: Flow Cytometry, Activation Assay

    En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P

    Journal: International Journal of Nanomedicine

    Article Title: Endothelial glycocalyx conditions influence nanoparticle uptake for passive targeting

    doi: 10.2147/IJN.S106299

    Figure Lengend Snippet: En face and cross sections of RFPEC in which HS was immunostained in green and cell nuclei in blue. Notes: ( A ) Control, ( B ) low serum, ( C ) Hep III, and ( D ) Hep III + HS. ( E ) Thickness measurements of the glycocalyx when observing only the HS component by binding the 10E4 epitope HS antibody to the glycosaminoglycan under control, low serum, Hep III, and Hep III + HS treatments. HS thickness decreased significantly for all treatments. ( F ) HS coverage of RFPEC after various treatments. HS coverage decreased significantly after heparinase III treatment and recovered after HS addition. * P

    Article Snippet: For the degraded glycocalyx configuration, cultures were pretreated with DMEM/10% FBS/1% penicillin-streptomycin containing a 2.5×10−6 IU heparinase III (Hep III) (IBEX Pharmaceutical, Montreal, QC, Canada) enzyme for 2 hours, selectively degrading the HS glycosaminoglycans of the glycocalyx.

    Techniques: Binding Assay