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Antagonistic effect of A. persicinum on H. <t>vastatrix</t> (A): Hv, inoculation with H. vastatrix urediniospores. (B): Hy, inoculation with strain HY85. (C): Hv 0 + Hy 0 , simultaneous inoculation with H. vastatrix urediniospores and strain HY85. (D): Hv 0 + Hy 72 , inoculation with H. vastatrix urediniospores first, followed by inoculation with strain HY85 after 72 h. (E): Hy 0 + Hv 72 , inoculation with HY85 first, followed by inoculation with H .vastatrix urediniospores after 72 h.
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Antagonistic effect of A. persicinum on H. <t>vastatrix</t> (A): Hv, inoculation with H. vastatrix urediniospores. (B): Hy, inoculation with strain HY85. (C): Hv 0 + Hy 0 , simultaneous inoculation with H. vastatrix urediniospores and strain HY85. (D): Hv 0 + Hy 72 , inoculation with H. vastatrix urediniospores first, followed by inoculation with strain HY85 after 72 h. (E): Hy 0 + Hv 72 , inoculation with HY85 first, followed by inoculation with H .vastatrix urediniospores after 72 h.
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Antagonistic effect of A. persicinum on H. <t>vastatrix</t> (A): Hv, inoculation with H. vastatrix urediniospores. (B): Hy, inoculation with strain HY85. (C): Hv 0 + Hy 0 , simultaneous inoculation with H. vastatrix urediniospores and strain HY85. (D): Hv 0 + Hy 72 , inoculation with H. vastatrix urediniospores first, followed by inoculation with strain HY85 after 72 h. (E): Hy 0 + Hv 72 , inoculation with HY85 first, followed by inoculation with H .vastatrix urediniospores after 72 h.
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Microsynth ag its3mix1-5 (its2 forward primer)
Antagonistic effect of A. persicinum on H. <t>vastatrix</t> (A): Hv, inoculation with H. vastatrix urediniospores. (B): Hy, inoculation with strain HY85. (C): Hv 0 + Hy 0 , simultaneous inoculation with H. vastatrix urediniospores and strain HY85. (D): Hv 0 + Hy 72 , inoculation with H. vastatrix urediniospores first, followed by inoculation with strain HY85 after 72 h. (E): Hy 0 + Hv 72 , inoculation with HY85 first, followed by inoculation with H .vastatrix urediniospores after 72 h.
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Illumina Inc its2 libraries
Antagonistic effect of A. persicinum on H. <t>vastatrix</t> (A): Hv, inoculation with H. vastatrix urediniospores. (B): Hy, inoculation with strain HY85. (C): Hv 0 + Hy 0 , simultaneous inoculation with H. vastatrix urediniospores and strain HY85. (D): Hv 0 + Hy 72 , inoculation with H. vastatrix urediniospores first, followed by inoculation with strain HY85 after 72 h. (E): Hy 0 + Hv 72 , inoculation with HY85 first, followed by inoculation with H .vastatrix urediniospores after 72 h.
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Antagonistic effect of A. persicinum on H. vastatrix (A): Hv, inoculation with H. vastatrix urediniospores. (B): Hy, inoculation with strain HY85. (C): Hv 0 + Hy 0 , simultaneous inoculation with H. vastatrix urediniospores and strain HY85. (D): Hv 0 + Hy 72 , inoculation with H. vastatrix urediniospores first, followed by inoculation with strain HY85 after 72 h. (E): Hy 0 + Hv 72 , inoculation with HY85 first, followed by inoculation with H .vastatrix urediniospores after 72 h.

Journal: FEMS Microbiology Ecology

Article Title: Identification and potential of the hyperparasite Acremonium persicinum as biocontrol agent against coffee leaf rust

doi: 10.1093/femsec/fiaf064

Figure Lengend Snippet: Antagonistic effect of A. persicinum on H. vastatrix (A): Hv, inoculation with H. vastatrix urediniospores. (B): Hy, inoculation with strain HY85. (C): Hv 0 + Hy 0 , simultaneous inoculation with H. vastatrix urediniospores and strain HY85. (D): Hv 0 + Hy 72 , inoculation with H. vastatrix urediniospores first, followed by inoculation with strain HY85 after 72 h. (E): Hy 0 + Hv 72 , inoculation with HY85 first, followed by inoculation with H .vastatrix urediniospores after 72 h.

Article Snippet: A set of specific primers and probes targeting the ITS2 sequence of H. vastatrix was designed using the online website ( https://www.genscript.com/tools/real-time-pcr-taqman-primer-design-tool , accessed 28 February 2024).

Techniques: