isradipine  (Alomone Labs)


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    Structured Review

    Alomone Labs isradipine
    LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and <t>isradipine</t> treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p
    Isradipine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isradipine/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isradipine - by Bioz Stars, 2022-01
    86/100 stars

    Images

    1) Product Images from "Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels"

    Article Title: Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels

    Journal: Molecular psychiatry

    doi: 10.1038/s41380-019-0513-2

    LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and isradipine treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p
    Figure Legend Snippet: LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and isradipine treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p

    Techniques Used: Conditioned Place Preference, SPR Assay, Mouse Assay

    Cocaine- and stress-primed reinstatement recruit the PrL→NAcC projection, a) C57BL/6J mice were injected with a Cre-dependent AAV expressing GCaMP6s into unilateral PrL and a retrograde AAV expressing Cre recombinase was injected into ipsilateral NAcC. An optic fiber was implanted above the PrL, which could be attached to a patch cord to record Ca 2+ signals, b) Representative image of GCaMP6s expressing in the PrL. Optic fiber location is outlined in white. MO, medial orbitofrontal cortex; Cg, cingulate cortex, c) Experimental timeline for fiber photometry recording during cocaine CPP. Ca 2+ imaging was conducted during the baseline test, acquisition test, extinction test, and CPR/SPR. Mice were treated with vehicle or isradipine to block LTCCs prior to CPR/SPR recording, d) Representative fiber photometry trace of a vehicle-treated mouse during CPR showing Ca 2+ transients prior to cocaine-paired entries (red) but not saline-paired entries (blue). Grey, middle CPP chamber, e) The average amplitude (% ΔF/F) of PrL→NAcC fiber photometry signal 5 seconds prior to entry into the cocaine-paired chamber was significantly higher as compared to entries into the saline-paired chamber in vehicle-treated mice (** p
    Figure Legend Snippet: Cocaine- and stress-primed reinstatement recruit the PrL→NAcC projection, a) C57BL/6J mice were injected with a Cre-dependent AAV expressing GCaMP6s into unilateral PrL and a retrograde AAV expressing Cre recombinase was injected into ipsilateral NAcC. An optic fiber was implanted above the PrL, which could be attached to a patch cord to record Ca 2+ signals, b) Representative image of GCaMP6s expressing in the PrL. Optic fiber location is outlined in white. MO, medial orbitofrontal cortex; Cg, cingulate cortex, c) Experimental timeline for fiber photometry recording during cocaine CPP. Ca 2+ imaging was conducted during the baseline test, acquisition test, extinction test, and CPR/SPR. Mice were treated with vehicle or isradipine to block LTCCs prior to CPR/SPR recording, d) Representative fiber photometry trace of a vehicle-treated mouse during CPR showing Ca 2+ transients prior to cocaine-paired entries (red) but not saline-paired entries (blue). Grey, middle CPP chamber, e) The average amplitude (% ΔF/F) of PrL→NAcC fiber photometry signal 5 seconds prior to entry into the cocaine-paired chamber was significantly higher as compared to entries into the saline-paired chamber in vehicle-treated mice (** p

    Techniques Used: Mouse Assay, Injection, Expressing, Imaging, SPR Assay, Blocking Assay

    2) Product Images from "CaMKII inactivation by extracellular Ca2+ depletion in dorsal root ganglion neurons"

    Article Title: CaMKII inactivation by extracellular Ca2+ depletion in dorsal root ganglion neurons

    Journal: Cell calcium

    doi: 10.1016/j.ceca.2006.01.005

    Activation of CaMKII is independent of VGCC-mediated Ca 2+ influx. Pre-incubation with either the L-type channel blockers isradipine or SR 33805 ( n = 6) at 10 μM failed to block CaMKII activation. Additionally, the Na + /Ca 2+ -exchanger inhibitor KB-R7943 at 10 μM ( n = 6) also did not affect activation of CaMKII by Ca 2+ O -stimulation. Basal phosphorylation at 286/287 Thr in 0 Ca 2+ was 12 ± 1% of stimulation by 1.8 Ca 2+ O . Neither compound significantly affected Ca 2+ -stimulation ( n = 6) suggesting that changes in [Ca 2+ ] O act at a site independent of VGCC.
    Figure Legend Snippet: Activation of CaMKII is independent of VGCC-mediated Ca 2+ influx. Pre-incubation with either the L-type channel blockers isradipine or SR 33805 ( n = 6) at 10 μM failed to block CaMKII activation. Additionally, the Na + /Ca 2+ -exchanger inhibitor KB-R7943 at 10 μM ( n = 6) also did not affect activation of CaMKII by Ca 2+ O -stimulation. Basal phosphorylation at 286/287 Thr in 0 Ca 2+ was 12 ± 1% of stimulation by 1.8 Ca 2+ O . Neither compound significantly affected Ca 2+ -stimulation ( n = 6) suggesting that changes in [Ca 2+ ] O act at a site independent of VGCC.

    Techniques Used: Activation Assay, Incubation, Blocking Assay, Activated Clotting Time Assay

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    Alomone Labs isradipine
    LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and <t>isradipine</t> treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p
    Isradipine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isradipine/product/Alomone Labs
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isradipine - by Bioz Stars, 2022-01
    86/100 stars
      Buy from Supplier

    80
    Alomone Labs l type calcium channel blocker isradipine
    LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and <t>isradipine</t> treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p
    L Type Calcium Channel Blocker Isradipine, supplied by Alomone Labs, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/l type calcium channel blocker isradipine/product/Alomone Labs
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    l type calcium channel blocker isradipine - by Bioz Stars, 2022-01
    80/100 stars
      Buy from Supplier

    Image Search Results


    LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and isradipine treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p

    Journal: Molecular psychiatry

    Article Title: Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels

    doi: 10.1038/s41380-019-0513-2

    Figure Lengend Snippet: LTCCs are required for cocaine- and stress-primed reinstatement of cocaine conditioned place preference, a) Experimental timeline of cocaine conditioned place preference (CPP) acquisition, extinction and cocaine-primed (CPR) or stress-primed (SPR) reinstatement protocol and isradipine treatment, b) Prior to treatment with either vehicle (pre-vehicle) or isradipine (pre-isradipine), all mice acquired (* p

    Article Snippet: Isradipine (Alomone Labs) was dissolved in saline (16% ethanol) at a concentration of 0.12 mg/ml to be injected i.p. at 0.01 ml/g body weight for a final dosage of 1.2 mg/kg.

    Techniques: Conditioned Place Preference, SPR Assay, Mouse Assay

    Cocaine- and stress-primed reinstatement recruit the PrL→NAcC projection, a) C57BL/6J mice were injected with a Cre-dependent AAV expressing GCaMP6s into unilateral PrL and a retrograde AAV expressing Cre recombinase was injected into ipsilateral NAcC. An optic fiber was implanted above the PrL, which could be attached to a patch cord to record Ca 2+ signals, b) Representative image of GCaMP6s expressing in the PrL. Optic fiber location is outlined in white. MO, medial orbitofrontal cortex; Cg, cingulate cortex, c) Experimental timeline for fiber photometry recording during cocaine CPP. Ca 2+ imaging was conducted during the baseline test, acquisition test, extinction test, and CPR/SPR. Mice were treated with vehicle or isradipine to block LTCCs prior to CPR/SPR recording, d) Representative fiber photometry trace of a vehicle-treated mouse during CPR showing Ca 2+ transients prior to cocaine-paired entries (red) but not saline-paired entries (blue). Grey, middle CPP chamber, e) The average amplitude (% ΔF/F) of PrL→NAcC fiber photometry signal 5 seconds prior to entry into the cocaine-paired chamber was significantly higher as compared to entries into the saline-paired chamber in vehicle-treated mice (** p

    Journal: Molecular psychiatry

    Article Title: Cocaine- and stress-primed reinstatement of drug-associated memories elicit differential behavioral and frontostriatal circuit activity patterns via recruitment of L-type Ca2+ channels

    doi: 10.1038/s41380-019-0513-2

    Figure Lengend Snippet: Cocaine- and stress-primed reinstatement recruit the PrL→NAcC projection, a) C57BL/6J mice were injected with a Cre-dependent AAV expressing GCaMP6s into unilateral PrL and a retrograde AAV expressing Cre recombinase was injected into ipsilateral NAcC. An optic fiber was implanted above the PrL, which could be attached to a patch cord to record Ca 2+ signals, b) Representative image of GCaMP6s expressing in the PrL. Optic fiber location is outlined in white. MO, medial orbitofrontal cortex; Cg, cingulate cortex, c) Experimental timeline for fiber photometry recording during cocaine CPP. Ca 2+ imaging was conducted during the baseline test, acquisition test, extinction test, and CPR/SPR. Mice were treated with vehicle or isradipine to block LTCCs prior to CPR/SPR recording, d) Representative fiber photometry trace of a vehicle-treated mouse during CPR showing Ca 2+ transients prior to cocaine-paired entries (red) but not saline-paired entries (blue). Grey, middle CPP chamber, e) The average amplitude (% ΔF/F) of PrL→NAcC fiber photometry signal 5 seconds prior to entry into the cocaine-paired chamber was significantly higher as compared to entries into the saline-paired chamber in vehicle-treated mice (** p

    Article Snippet: Isradipine (Alomone Labs) was dissolved in saline (16% ethanol) at a concentration of 0.12 mg/ml to be injected i.p. at 0.01 ml/g body weight for a final dosage of 1.2 mg/kg.

    Techniques: Mouse Assay, Injection, Expressing, Imaging, SPR Assay, Blocking Assay

    Activation of CaMKII is independent of VGCC-mediated Ca 2+ influx. Pre-incubation with either the L-type channel blockers isradipine or SR 33805 ( n = 6) at 10 μM failed to block CaMKII activation. Additionally, the Na + /Ca 2+ -exchanger inhibitor KB-R7943 at 10 μM ( n = 6) also did not affect activation of CaMKII by Ca 2+ O -stimulation. Basal phosphorylation at 286/287 Thr in 0 Ca 2+ was 12 ± 1% of stimulation by 1.8 Ca 2+ O . Neither compound significantly affected Ca 2+ -stimulation ( n = 6) suggesting that changes in [Ca 2+ ] O act at a site independent of VGCC.

    Journal: Cell calcium

    Article Title: CaMKII inactivation by extracellular Ca2+ depletion in dorsal root ganglion neurons

    doi: 10.1016/j.ceca.2006.01.005

    Figure Lengend Snippet: Activation of CaMKII is independent of VGCC-mediated Ca 2+ influx. Pre-incubation with either the L-type channel blockers isradipine or SR 33805 ( n = 6) at 10 μM failed to block CaMKII activation. Additionally, the Na + /Ca 2+ -exchanger inhibitor KB-R7943 at 10 μM ( n = 6) also did not affect activation of CaMKII by Ca 2+ O -stimulation. Basal phosphorylation at 286/287 Thr in 0 Ca 2+ was 12 ± 1% of stimulation by 1.8 Ca 2+ O . Neither compound significantly affected Ca 2+ -stimulation ( n = 6) suggesting that changes in [Ca 2+ ] O act at a site independent of VGCC.

    Article Snippet: The following drugs were used: isradipine (Alomone Labs, Jerusalem, Israel), flunarizine, ionomycin, ω-agatoxin-IVA, and ω-conotoxin-GVIA (EMD Biosciences Inc., San Diego, CA), Fluo-4/AM and Indo-1/AM (Molecular Probes, Eugene OR), caffeine, EGTA, gadolinium chloride, lanthanum chloride, nickel chloride, and nifedipine (Sigma, St. Louis, MO), KB-R7943, MRS 1845, Ruthenium Red (RR), SK & F 96365, and SR 33805 (Tocris Cookson, Ellisville, MO).

    Techniques: Activation Assay, Incubation, Blocking Assay, Activated Clotting Time Assay