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SouthernBiotech isotype igg1 igg2 igg3 or igg4
Isotype Igg1 Igg2 Igg3 Or Igg4, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/isotype igg1 igg2 igg3 or igg4/product/SouthernBiotech
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
isotype igg1 igg2 igg3 or igg4 - by Bioz Stars, 2020-07
86/100 stars

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biotinylated mouse anti-human igg

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Article Title: Exploring necrotizing autoimmune myopathies with a novel immunoassay for anti-3-hydroxy-3-methyl-glutaryl-CoA reductase autoantibodies
Article Snippet: .. Biotinylated mouse anti-human IgG- or isotype (IgG1, IgG2, IgG3 or IgG4)-specific secondary Ab (Southern Biotech, Birmingham, AL, USA) were added at 1/2,000 dilution and incubated for 1 h at room temperature under shaking. .. After washing, beads were incubated with 50 μl of streptavidin-R-phycoerythrin (Qiagen, Venlo, The Netherlands) at 1/1,000 dilution for 15 min.

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  • 94
    SouthernBiotech igg1
    Production of Sm29-specific antibodies in immunized mice. Sera from mice were obtained 15 days after each immunization dose and were assessed to determine the levels of IgG (A) , <t>IgG1</t> (B) , IgG2a (C) , and IgE (D) antibodies against rSm29 in the animals inoculated with alum (open bars), MPLA (bright-gray bars), Sm29Alum (black bars) or Sm29/MPLA (dark-gray bar). Significant differences between adjuvant control and experimental groups are indicated by an asterisk ( p
    Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 94/100, based on 899 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/igg1/product/SouthernBiotech
    Average 94 stars, based on 899 article reviews
    Price from $9.99 to $1999.99
    igg1 - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    93
    SouthernBiotech goat anti mouse igg1
    Isotype switching and plasmablast differentiation in Igh CGG/+ mice in vitro. (a) In vitro isotype switching. Purified Igh CGG/+ (CD45.2/2) and WT (CD45.1/1) B cells were cocultured in vitro with LPS and IL-4 to induce isotype switching to <t>IgG</t> 1 . Histogram shows IgG 1 staining by flow cytometry on day 3 of culture. Data are from three independent experiments and are quantified in the graph; proportions of IgG 1 + cells in Igh CGG/+ and WT B cells from the same experiment are connected by a line. P = 0.74 (unpaired t test). (b) AFC cell formation (as defined by CD138 expression) and IgG 1 secretion in purified B cells stimulated with LPS and IL-4 in vitro. Cells were presorted as in Fig. 3 a , and non–CGG-binding B cells were excluded from Igh CGG/+ cultures. Histograms are representative of, and graphs show pooled data for two independent experiments. n = 3 mice for WT and n = 6 mice for Igh CGG/+ CGG binding B cells. P = 0.33 for CD138 expression and P = 0.37 for supernatant IgG 1 . Numbers within flow plots indicate percentage of cells in the designated gate.
    Goat Anti Mouse Igg1, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 56 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/goat anti mouse igg1/product/SouthernBiotech
    Average 93 stars, based on 56 article reviews
    Price from $9.99 to $1999.99
    goat anti mouse igg1 - by Bioz Stars, 2020-07
    93/100 stars
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    93
    SouthernBiotech anti porcine cd8 pe mab
    PRRSV-induced IL-1Ra inhibited lymphocyte proliferation. PRRSV-induced IL-1Ra inhibited PHA-induced proliferation of the (A) CD4 + , (B) <t>CD8</t> + , and (C) CD4 + CD8 + subpopulations. PRRSV-induced IL-1Ra inhibited CSFV-specific proliferations of the (D) CD4 + , (E) CD8 + , and (F) CD4 + CD8 + subpopulations. The supernatants obtained from type 2 PRRSV or mock (MARC-145 cell lysate) were pretreated with anti-IL-1Ra Ab for 2 h prior to addition into the culture. PBL or PBMC were culture with PHA, CSFV or controls for 96 h, in the presence of the pretreated supernatants. ± indicates presence/absence of indicated treatment within the culture. Data represents mean ± SD from 5 pigs. Statistical significance was analyzed using ANOVA followed by Tukey's test. * indicates significant difference at p
    Anti Porcine Cd8 Pe Mab, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti porcine cd8 pe mab/product/SouthernBiotech
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti porcine cd8 pe mab - by Bioz Stars, 2020-07
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    85
    SouthernBiotech anti falvac 1a immunoglobulin g igg
    Antibody responses to <t>FALVAC-1A</t> vaccine formulations in congenic mice. Mice were immunized with 10 μg FALVAC-1A/dose formulated in copolymer CRL-1005 (Copol/FAL), Montanide ISA-720 (ISA-720/FAL), QS-21 (QS-21/FAL), aluminum (AlPO4/FAL), or PBS (FALVAC-1A) on days 0, 14, and 28. Total <t>IgG</t> antibody titers (geometric mean of 10 mice) were determined as the geometric mean of the reciprocal dilution where the OD 450 of the titration curve equaled 0.1 (the maximum value of preimmune sera at 1:100).
    Anti Falvac 1a Immunoglobulin G Igg, supplied by SouthernBiotech, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti falvac 1a immunoglobulin g igg/product/SouthernBiotech
    Average 85 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti falvac 1a immunoglobulin g igg - by Bioz Stars, 2020-07
    85/100 stars
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    Production of Sm29-specific antibodies in immunized mice. Sera from mice were obtained 15 days after each immunization dose and were assessed to determine the levels of IgG (A) , IgG1 (B) , IgG2a (C) , and IgE (D) antibodies against rSm29 in the animals inoculated with alum (open bars), MPLA (bright-gray bars), Sm29Alum (black bars) or Sm29/MPLA (dark-gray bar). Significant differences between adjuvant control and experimental groups are indicated by an asterisk ( p

    Journal: Frontiers in Immunology

    Article Title: A Strong Humoral Immune Response Induced by a Vaccine Formulation Containing rSm29 Adsorbed to Alum Is Associated With Protection Against Schistosoma mansoni Reinfection in Mice

    doi: 10.3389/fimmu.2018.02488

    Figure Lengend Snippet: Production of Sm29-specific antibodies in immunized mice. Sera from mice were obtained 15 days after each immunization dose and were assessed to determine the levels of IgG (A) , IgG1 (B) , IgG2a (C) , and IgE (D) antibodies against rSm29 in the animals inoculated with alum (open bars), MPLA (bright-gray bars), Sm29Alum (black bars) or Sm29/MPLA (dark-gray bar). Significant differences between adjuvant control and experimental groups are indicated by an asterisk ( p

    Article Snippet: One hundred microliters of each serum sample was diluted 1:1,000 (for IgG, IgG1, and IgG2a) or 1:40 (for IgE) and added to the plates for 1 h. Finally, the plates were incubated with peroxidase-conjugated anti-mouse IgG (1:10,000), IgG1, (1:10,000), or IgG2a (1:8,000) (Southern Biotech, Birmingham, AL, United States), for 1 h at 25°C.

    Techniques: Mouse Assay

    Isotype switching and plasmablast differentiation in Igh CGG/+ mice in vitro. (a) In vitro isotype switching. Purified Igh CGG/+ (CD45.2/2) and WT (CD45.1/1) B cells were cocultured in vitro with LPS and IL-4 to induce isotype switching to IgG 1 . Histogram shows IgG 1 staining by flow cytometry on day 3 of culture. Data are from three independent experiments and are quantified in the graph; proportions of IgG 1 + cells in Igh CGG/+ and WT B cells from the same experiment are connected by a line. P = 0.74 (unpaired t test). (b) AFC cell formation (as defined by CD138 expression) and IgG 1 secretion in purified B cells stimulated with LPS and IL-4 in vitro. Cells were presorted as in Fig. 3 a , and non–CGG-binding B cells were excluded from Igh CGG/+ cultures. Histograms are representative of, and graphs show pooled data for two independent experiments. n = 3 mice for WT and n = 6 mice for Igh CGG/+ CGG binding B cells. P = 0.33 for CD138 expression and P = 0.37 for supernatant IgG 1 . Numbers within flow plots indicate percentage of cells in the designated gate.

    Journal: The Journal of Experimental Medicine

    Article Title: One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

    doi: 10.1084/jem.20172064

    Figure Lengend Snippet: Isotype switching and plasmablast differentiation in Igh CGG/+ mice in vitro. (a) In vitro isotype switching. Purified Igh CGG/+ (CD45.2/2) and WT (CD45.1/1) B cells were cocultured in vitro with LPS and IL-4 to induce isotype switching to IgG 1 . Histogram shows IgG 1 staining by flow cytometry on day 3 of culture. Data are from three independent experiments and are quantified in the graph; proportions of IgG 1 + cells in Igh CGG/+ and WT B cells from the same experiment are connected by a line. P = 0.74 (unpaired t test). (b) AFC cell formation (as defined by CD138 expression) and IgG 1 secretion in purified B cells stimulated with LPS and IL-4 in vitro. Cells were presorted as in Fig. 3 a , and non–CGG-binding B cells were excluded from Igh CGG/+ cultures. Histograms are representative of, and graphs show pooled data for two independent experiments. n = 3 mice for WT and n = 6 mice for Igh CGG/+ CGG binding B cells. P = 0.33 for CD138 expression and P = 0.37 for supernatant IgG 1 . Numbers within flow plots indicate percentage of cells in the designated gate.

    Article Snippet: Isotype-specific ELISAs were performed by coating with goat anti–mouse IgG1 (1070–01; SouthernBiotech) at 1 µg/ml and detecting with goat anti–mouse IgG1 (1070–05; SouthernBiotech) at 1:5,000 dilution ( ).

    Techniques: Mouse Assay, In Vitro, Purification, Staining, Flow Cytometry, Cytometry, Expressing, Binding Assay

    CSR, SHM, and antigen-driven selection in Igh CGG/+ B cells. (a) Igh CGG/+ B cells (CD45.2/2) were adoptively transferred into WT (CD45.1/1) recipients, which were immunized 1 d later with CGG in alum. CSR to IgG 1 was determined in GC B cells (CD19 + TCRβ – Fas + CD38 lo ) from donor (CD45.2/2) and recipient (CD45.1/1) mice by flow cytometry. Graph shows percentage of IgG 1 + GC B cells in five mice from two independent experiments. Proportions of switched cells in the same mouse are connected by a line. P = 0.23 (unpaired t test). Numbers within flow plots indicate percentage of cells in the designated gate. (b) Left: Serum titer of IgG 1 + /human Cκ + Ig in three mice adoptively transferred with Igh CGG/+ B cells and immunized with CGG. Graph shows data for three mice from two independent experiments. Right: Western blot for human Igκ in serum of mice adoptively transferred with Igh CGG/+ B cells and immunized with CGG 14 d after immunization. NR, nonreducing conditions; R, reducing conditions (DTT). Blot is representative of two experiments. (c–f) GC B cells from adoptive transfer experiments as in panel a were single sorted, and the entire Igh CGG/+ allele was PCR-amplified and sequenced. (c) Nucleotide mutation frequencies along the Igh CGG/+ locus, calculated as the fraction of times a particular nucleotide was mutated from the original sequence. (d) Total mutation frequency per region (normalized to sequence length). Each symbol represents one cell. (e) Number of nucleotide mutations in VDJ H and VJκ in Igh CGG/+ GC B cells compared with WT GC B cells carrying the same rearrangement selected by CGG immunization, as reported previously ( Tas et al., 2016 ; only data from LN#2 GC2 in Fig. 4 from Tas et al., 2016 are analyzed). Data from two transfers of Igh CGG/+ B cells are pooled and compared with the WT. **, P ≤ 0.001; ****, P ≤ 0.0001 (unpaired t test). (f) Comparison of nucleotide mutation patterns between Igh CGG/+ B cells and WT GC B cells carrying the same rearrangement. Mutation patterns are shown for FR1 to FR4 regions of IgH and CDR1 to FR4 regions of Igκ. Shared nucleotide mutation positions are shown in red. Asterisk indicates a C119 > G mutation, which confers an approximately eightfold gain in affinity. Data from two transfers of Igh CGG/+ B cells are pooled and compared with the WT. (g) Reconstructed phylogeny of Igh CGG/+ GC B cell VJ Igκ sequences from multiple GCs in one LN. Clades of expanded clones are indicated in red, green, and blue. Cells containing the high-affinity C119 > G mutation are indicated in green.

    Journal: The Journal of Experimental Medicine

    Article Title: One-step generation of monoclonal B cell receptor mice capable of isotype switching and somatic hypermutation

    doi: 10.1084/jem.20172064

    Figure Lengend Snippet: CSR, SHM, and antigen-driven selection in Igh CGG/+ B cells. (a) Igh CGG/+ B cells (CD45.2/2) were adoptively transferred into WT (CD45.1/1) recipients, which were immunized 1 d later with CGG in alum. CSR to IgG 1 was determined in GC B cells (CD19 + TCRβ – Fas + CD38 lo ) from donor (CD45.2/2) and recipient (CD45.1/1) mice by flow cytometry. Graph shows percentage of IgG 1 + GC B cells in five mice from two independent experiments. Proportions of switched cells in the same mouse are connected by a line. P = 0.23 (unpaired t test). Numbers within flow plots indicate percentage of cells in the designated gate. (b) Left: Serum titer of IgG 1 + /human Cκ + Ig in three mice adoptively transferred with Igh CGG/+ B cells and immunized with CGG. Graph shows data for three mice from two independent experiments. Right: Western blot for human Igκ in serum of mice adoptively transferred with Igh CGG/+ B cells and immunized with CGG 14 d after immunization. NR, nonreducing conditions; R, reducing conditions (DTT). Blot is representative of two experiments. (c–f) GC B cells from adoptive transfer experiments as in panel a were single sorted, and the entire Igh CGG/+ allele was PCR-amplified and sequenced. (c) Nucleotide mutation frequencies along the Igh CGG/+ locus, calculated as the fraction of times a particular nucleotide was mutated from the original sequence. (d) Total mutation frequency per region (normalized to sequence length). Each symbol represents one cell. (e) Number of nucleotide mutations in VDJ H and VJκ in Igh CGG/+ GC B cells compared with WT GC B cells carrying the same rearrangement selected by CGG immunization, as reported previously ( Tas et al., 2016 ; only data from LN#2 GC2 in Fig. 4 from Tas et al., 2016 are analyzed). Data from two transfers of Igh CGG/+ B cells are pooled and compared with the WT. **, P ≤ 0.001; ****, P ≤ 0.0001 (unpaired t test). (f) Comparison of nucleotide mutation patterns between Igh CGG/+ B cells and WT GC B cells carrying the same rearrangement. Mutation patterns are shown for FR1 to FR4 regions of IgH and CDR1 to FR4 regions of Igκ. Shared nucleotide mutation positions are shown in red. Asterisk indicates a C119 > G mutation, which confers an approximately eightfold gain in affinity. Data from two transfers of Igh CGG/+ B cells are pooled and compared with the WT. (g) Reconstructed phylogeny of Igh CGG/+ GC B cell VJ Igκ sequences from multiple GCs in one LN. Clades of expanded clones are indicated in red, green, and blue. Cells containing the high-affinity C119 > G mutation are indicated in green.

    Article Snippet: Isotype-specific ELISAs were performed by coating with goat anti–mouse IgG1 (1070–01; SouthernBiotech) at 1 µg/ml and detecting with goat anti–mouse IgG1 (1070–05; SouthernBiotech) at 1:5,000 dilution ( ).

    Techniques: Selection, Mouse Assay, Flow Cytometry, Cytometry, Western Blot, Adoptive Transfer Assay, Polymerase Chain Reaction, Amplification, Mutagenesis, Sequencing, Clone Assay

    PRRSV-induced IL-1Ra inhibited lymphocyte proliferation. PRRSV-induced IL-1Ra inhibited PHA-induced proliferation of the (A) CD4 + , (B) CD8 + , and (C) CD4 + CD8 + subpopulations. PRRSV-induced IL-1Ra inhibited CSFV-specific proliferations of the (D) CD4 + , (E) CD8 + , and (F) CD4 + CD8 + subpopulations. The supernatants obtained from type 2 PRRSV or mock (MARC-145 cell lysate) were pretreated with anti-IL-1Ra Ab for 2 h prior to addition into the culture. PBL or PBMC were culture with PHA, CSFV or controls for 96 h, in the presence of the pretreated supernatants. ± indicates presence/absence of indicated treatment within the culture. Data represents mean ± SD from 5 pigs. Statistical significance was analyzed using ANOVA followed by Tukey's test. * indicates significant difference at p

    Journal: Frontiers in Immunology

    Article Title: Negative Immunomodulatory Effects of Type 2 Porcine Reproductive and Respiratory Syndrome Virus-Induced Interleukin-1 Receptor Antagonist on Porcine Innate and Adaptive Immune Functions

    doi: 10.3389/fimmu.2019.00579

    Figure Lengend Snippet: PRRSV-induced IL-1Ra inhibited lymphocyte proliferation. PRRSV-induced IL-1Ra inhibited PHA-induced proliferation of the (A) CD4 + , (B) CD8 + , and (C) CD4 + CD8 + subpopulations. PRRSV-induced IL-1Ra inhibited CSFV-specific proliferations of the (D) CD4 + , (E) CD8 + , and (F) CD4 + CD8 + subpopulations. The supernatants obtained from type 2 PRRSV or mock (MARC-145 cell lysate) were pretreated with anti-IL-1Ra Ab for 2 h prior to addition into the culture. PBL or PBMC were culture with PHA, CSFV or controls for 96 h, in the presence of the pretreated supernatants. ± indicates presence/absence of indicated treatment within the culture. Data represents mean ± SD from 5 pigs. Statistical significance was analyzed using ANOVA followed by Tukey's test. * indicates significant difference at p

    Article Snippet: Anti-porcine SLA-DR mAb (1053H2-18, IgG2a), anti-porcine CD3-FITC mAb (BB23-8E6, IgG2b), biotinylated anti-porcine CD4 mAb (74-12-4, IgG2b), and anti-porcine CD8-PE mAb (76-2-11, IgG2a) were purchased from Southern Biotech (Birmingham, AL, USA).

    Techniques:

    Antibody responses to FALVAC-1A vaccine formulations in congenic mice. Mice were immunized with 10 μg FALVAC-1A/dose formulated in copolymer CRL-1005 (Copol/FAL), Montanide ISA-720 (ISA-720/FAL), QS-21 (QS-21/FAL), aluminum (AlPO4/FAL), or PBS (FALVAC-1A) on days 0, 14, and 28. Total IgG antibody titers (geometric mean of 10 mice) were determined as the geometric mean of the reciprocal dilution where the OD 450 of the titration curve equaled 0.1 (the maximum value of preimmune sera at 1:100).

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Immune Responses of Mice with Different Genetic Backgrounds to Improved Multiepitope, Multitarget Malaria Vaccine Candidate Antigen FALVAC-1A ▿

    doi: 10.1128/CVI.00164-08

    Figure Lengend Snippet: Antibody responses to FALVAC-1A vaccine formulations in congenic mice. Mice were immunized with 10 μg FALVAC-1A/dose formulated in copolymer CRL-1005 (Copol/FAL), Montanide ISA-720 (ISA-720/FAL), QS-21 (QS-21/FAL), aluminum (AlPO4/FAL), or PBS (FALVAC-1A) on days 0, 14, and 28. Total IgG antibody titers (geometric mean of 10 mice) were determined as the geometric mean of the reciprocal dilution where the OD 450 of the titration curve equaled 0.1 (the maximum value of preimmune sera at 1:100).

    Article Snippet: To determine the titers of total anti-FALVAC-1A immunoglobulin G (IgG), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Southern Biotech, Birmingham, AL) was used at a dilution of 1:5,000.

    Techniques: Mouse Assay, Titration

    Reactivity of mice anti-FALVAC-1A sera with P. falciparum sporozoites and blood-stage parasites. Day 42 sera were tested by IFA. Each bar represents the geometric mean titer of the total IgG antibody titers for 10 mice induced by the given adjuvant formulation.

    Journal: Clinical and Vaccine Immunology : CVI

    Article Title: Immune Responses of Mice with Different Genetic Backgrounds to Improved Multiepitope, Multitarget Malaria Vaccine Candidate Antigen FALVAC-1A ▿

    doi: 10.1128/CVI.00164-08

    Figure Lengend Snippet: Reactivity of mice anti-FALVAC-1A sera with P. falciparum sporozoites and blood-stage parasites. Day 42 sera were tested by IFA. Each bar represents the geometric mean titer of the total IgG antibody titers for 10 mice induced by the given adjuvant formulation.

    Article Snippet: To determine the titers of total anti-FALVAC-1A immunoglobulin G (IgG), horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (Southern Biotech, Birmingham, AL) was used at a dilution of 1:5,000.

    Techniques: Mouse Assay, Immunofluorescence