isolectin b 4  (Vector Laboratories)


Bioz Verified Symbol Vector Laboratories is a verified supplier
Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories isolectin b 4
    Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isolectin b 4/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isolectin b 4 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    biotin conjugated isolectin b4  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories biotin conjugated isolectin b4
    Biotin Conjugated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated isolectin b4/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated isolectin b4 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    lectins  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories lectins
    Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lectins - by Bioz Stars, 2024-06
    86/100 stars

    Images

    lectins  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories lectins
    Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lectins - by Bioz Stars, 2024-06
    86/100 stars

    Images

    lectins  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories lectins
    Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lectins - by Bioz Stars, 2024-06
    86/100 stars

    Images

    biotinylated lectins  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories biotinylated lectins
    <t> Lectins </t> used in this study, and the glycans to which they bind, according to CFG data. <t> Lectins </t> displayed binding to MVs are marked in grey. The three lectins marked with * displayed binding non-specifically to the glass slides, and those lectins along with the lectin marked # were only analyzed using flow cytometry. The structure of glycans was presented in the symbol nomenclature for glycans (SNFGs) format ( https://www.ncbi.nlm.nih.gov/glycans/snfg.html accessed on 26 February 2024) using Carbohydrate Structure Databases, 16 March 2024 found at http://csdb.glycoscience.ru/database/core/wizard.html .
    Biotinylated Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated lectins - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins"

    Article Title: Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

    Journal: International Journal of Molecular Sciences

    doi: 10.3390/ijms25115725

     Lectins  used in this study, and the glycans to which they bind, according to CFG data.  Lectins  displayed binding to MVs are marked in grey. The three lectins marked with * displayed binding non-specifically to the glass slides, and those lectins along with the lectin marked # were only analyzed using flow cytometry. The structure of glycans was presented in the symbol nomenclature for glycans (SNFGs) format ( https://www.ncbi.nlm.nih.gov/glycans/snfg.html accessed on 26 February 2024) using Carbohydrate Structure Databases, 16 March 2024 found at http://csdb.glycoscience.ru/database/core/wizard.html .
    Figure Legend Snippet: Lectins used in this study, and the glycans to which they bind, according to CFG data. Lectins displayed binding to MVs are marked in grey. The three lectins marked with * displayed binding non-specifically to the glass slides, and those lectins along with the lectin marked # were only analyzed using flow cytometry. The structure of glycans was presented in the symbol nomenclature for glycans (SNFGs) format ( https://www.ncbi.nlm.nih.gov/glycans/snfg.html accessed on 26 February 2024) using Carbohydrate Structure Databases, 16 March 2024 found at http://csdb.glycoscience.ru/database/core/wizard.html .

    Techniques Used: Binding Assay, Flow Cytometry

    Solid-phase detection of lectin binding to MVs. MVs were derived from EA.hy 926 cells and immobilized on glass slides (solid-phase assay), incubated with biotinylated lectins, and then visualized with Str-Alexa Fluor 555, as described in the Materials and Methods section.
    Figure Legend Snippet: Solid-phase detection of lectin binding to MVs. MVs were derived from EA.hy 926 cells and immobilized on glass slides (solid-phase assay), incubated with biotinylated lectins, and then visualized with Str-Alexa Fluor 555, as described in the Materials and Methods section.

    Techniques Used: Binding Assay, Derivative Assay, Incubation

    Flow cytometry detection of lectins DSA and SNA binding to glycosidase-treated MVs. MVs were incubated with either β-galactosidase or neuraminidase, washed and incubated with biotinylated lectins, as described in the Materials and Methods section. In the dot plots, forward scatter (FS) was plotted against the logarithm of fluorescence (FL1) and the number given in the rectangles (for the purple dots) indicate the percentage of lectin bound to MVs.
    Figure Legend Snippet: Flow cytometry detection of lectins DSA and SNA binding to glycosidase-treated MVs. MVs were incubated with either β-galactosidase or neuraminidase, washed and incubated with biotinylated lectins, as described in the Materials and Methods section. In the dot plots, forward scatter (FS) was plotted against the logarithm of fluorescence (FL1) and the number given in the rectangles (for the purple dots) indicate the percentage of lectin bound to MVs.

    Techniques Used: Flow Cytometry, Binding Assay, Incubation, Fluorescence

    Flow cytometry binding of lectins to EA.hy 926 cells. Cells were incubated with biotinylated lectins followed by Str-FITC, as described in the Materials and Methods Section. Results shown include data from four experiments and error bars represent standard deviations. A mean fluorescence <50 was considered as negative.
    Figure Legend Snippet: Flow cytometry binding of lectins to EA.hy 926 cells. Cells were incubated with biotinylated lectins followed by Str-FITC, as described in the Materials and Methods Section. Results shown include data from four experiments and error bars represent standard deviations. A mean fluorescence <50 was considered as negative.

    Techniques Used: Flow Cytometry, Binding Assay, Incubation, Fluorescence

    Binding of lectins to MVs derived from EA.hy 926 cells treated with inhibitors to N -glycosylation, DMJ ( A ) or O -glycosylation, GalNAcαBn ( B ). MVs were isolated from native cells and cells treated overnight with GalNAcαBn or DMJ. Flow cytometry results shown include data from two experiments and error bars represent standard deviations; *** p < 0.001, ** p < 0.01, * p < 0.1.
    Figure Legend Snippet: Binding of lectins to MVs derived from EA.hy 926 cells treated with inhibitors to N -glycosylation, DMJ ( A ) or O -glycosylation, GalNAcαBn ( B ). MVs were isolated from native cells and cells treated overnight with GalNAcαBn or DMJ. Flow cytometry results shown include data from two experiments and error bars represent standard deviations; *** p < 0.001, ** p < 0.01, * p < 0.1.

    Techniques Used: Binding Assay, Derivative Assay, Isolation, Flow Cytometry

    Binding of lectins to MVs released from the EA.hy 926 cells treated with enzymes. MVs were isolated from cells treated with collagenase, hyaluronidase, and trypsin. Flow cytometry results include data from three experiments; error bars represent standard deviations; * p < 0.1.
    Figure Legend Snippet: Binding of lectins to MVs released from the EA.hy 926 cells treated with enzymes. MVs were isolated from cells treated with collagenase, hyaluronidase, and trypsin. Flow cytometry results include data from three experiments; error bars represent standard deviations; * p < 0.1.

    Techniques Used: Binding Assay, Isolation, Flow Cytometry

    lectins  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories lectins
    top panel , Cell surface sialic acid expression in mCherry+ TKO mouse OC cells in culture as detected <t>by</t> <t>SNA,</t> MAL-I, MAL-II and PNA; bottom panel , mCherry+ TKO mouse OC cells were injected i.p. in C57BL/6 mice and omental tumors were dissociated, stained with anti-CD45 and FITC-tagged <t>lectins</t> (n=5). Histograms show FITC staining from CD45-negative population. Note loss of SNA-low population and increase in Mal-I and Mal-II upon in vivo tumor formation. Histograms show data from one mouse. Similar results were observed in other mice. Double sided arrow shows SNA levels in tumors is comparable to SNA levels in TKO SNAhigh cells.
    Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lectins - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Adipose microenvironment promotes hypersialylation of ovarian cancer cells"

    Article Title: Adipose microenvironment promotes hypersialylation of ovarian cancer cells

    Journal: bioRxiv

    doi: 10.1101/2024.05.13.593990

    top panel , Cell surface sialic acid expression in mCherry+ TKO mouse OC cells in culture as detected by SNA, MAL-I, MAL-II and PNA; bottom panel , mCherry+ TKO mouse OC cells were injected i.p. in C57BL/6 mice and omental tumors were dissociated, stained with anti-CD45 and FITC-tagged lectins (n=5). Histograms show FITC staining from CD45-negative population. Note loss of SNA-low population and increase in Mal-I and Mal-II upon in vivo tumor formation. Histograms show data from one mouse. Similar results were observed in other mice. Double sided arrow shows SNA levels in tumors is comparable to SNA levels in TKO SNAhigh cells.
    Figure Legend Snippet: top panel , Cell surface sialic acid expression in mCherry+ TKO mouse OC cells in culture as detected by SNA, MAL-I, MAL-II and PNA; bottom panel , mCherry+ TKO mouse OC cells were injected i.p. in C57BL/6 mice and omental tumors were dissociated, stained with anti-CD45 and FITC-tagged lectins (n=5). Histograms show FITC staining from CD45-negative population. Note loss of SNA-low population and increase in Mal-I and Mal-II upon in vivo tumor formation. Histograms show data from one mouse. Similar results were observed in other mice. Double sided arrow shows SNA levels in tumors is comparable to SNA levels in TKO SNAhigh cells.

    Techniques Used: Expressing, Injection, Staining, In Vivo

    anti isolectin b4 antibody conjugated to fluorescein  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories anti isolectin b4 antibody conjugated to fluorescein
    SKM injection promotes a transient increase in vasculature development. Tissue sections were stained against smooth muscle (ɑSMA, green), endothelial cells <t>(isolectin,</t> cyan), and nuclei (DAPI). Arterioles, double positive for ɑSMA and isolectin, were counted and assessed for lumen area. Representative images of stained tissue from saline (A) and SKM (B) treated animals at 7 days post-injection are shown (Scale bar: 100 µm). (C) At 7 days post-injection, SKM group demonstrated significantly higher vessel density throughout the whole tissue section (unpaired t -test, ***p<0.001). (D) The vessels in SKM injected animals demonstrated a significantly smaller overall size distribution at 7 days post-injection (Mann-Whitney test, ****p<0.0001). (F) At 4 weeks post-injection, there was a nonsignificant increase in vessel density within the scar area in SKM injected animals (unpaired t -test, p=0.079). (F) At 4 weeks post-injection, the smaller size distribution of vessels in the SKM group was maintained (Mann-Whitney test, ***p<0.001). n = 6 animals per group.
    Anti Isolectin B4 Antibody Conjugated To Fluorescein, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti isolectin b4 antibody conjugated to fluorescein/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti isolectin b4 antibody conjugated to fluorescein - by Bioz Stars, 2024-06
    86/100 stars

    Images

    1) Product Images from "Immunomodulatory extracellular matrix hydrogel induces tissue regeneration in a model of partial glossectomy"

    Article Title: Immunomodulatory extracellular matrix hydrogel induces tissue regeneration in a model of partial glossectomy

    Journal: Bioactive Materials

    doi: 10.1016/j.bioactmat.2024.05.001

    SKM injection promotes a transient increase in vasculature development. Tissue sections were stained against smooth muscle (ɑSMA, green), endothelial cells (isolectin, cyan), and nuclei (DAPI). Arterioles, double positive for ɑSMA and isolectin, were counted and assessed for lumen area. Representative images of stained tissue from saline (A) and SKM (B) treated animals at 7 days post-injection are shown (Scale bar: 100 µm). (C) At 7 days post-injection, SKM group demonstrated significantly higher vessel density throughout the whole tissue section (unpaired t -test, ***p<0.001). (D) The vessels in SKM injected animals demonstrated a significantly smaller overall size distribution at 7 days post-injection (Mann-Whitney test, ****p<0.0001). (F) At 4 weeks post-injection, there was a nonsignificant increase in vessel density within the scar area in SKM injected animals (unpaired t -test, p=0.079). (F) At 4 weeks post-injection, the smaller size distribution of vessels in the SKM group was maintained (Mann-Whitney test, ***p<0.001). n = 6 animals per group.
    Figure Legend Snippet: SKM injection promotes a transient increase in vasculature development. Tissue sections were stained against smooth muscle (ɑSMA, green), endothelial cells (isolectin, cyan), and nuclei (DAPI). Arterioles, double positive for ɑSMA and isolectin, were counted and assessed for lumen area. Representative images of stained tissue from saline (A) and SKM (B) treated animals at 7 days post-injection are shown (Scale bar: 100 µm). (C) At 7 days post-injection, SKM group demonstrated significantly higher vessel density throughout the whole tissue section (unpaired t -test, ***p<0.001). (D) The vessels in SKM injected animals demonstrated a significantly smaller overall size distribution at 7 days post-injection (Mann-Whitney test, ****p<0.0001). (F) At 4 weeks post-injection, there was a nonsignificant increase in vessel density within the scar area in SKM injected animals (unpaired t -test, p=0.079). (F) At 4 weeks post-injection, the smaller size distribution of vessels in the SKM group was maintained (Mann-Whitney test, ***p<0.001). n = 6 animals per group.

    Techniques Used: Injection, Staining, Saline, MANN-WHITNEY

    fitc conjugated isolectin b4  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories fitc conjugated isolectin b4
    Fitc Conjugated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated isolectin b4/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated isolectin b4 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    fitc isolectin b4  (Vector Laboratories)


    Bioz Verified Symbol Vector Laboratories is a verified supplier
    Bioz Manufacturer Symbol Vector Laboratories manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86

    Structured Review

    Vector Laboratories fitc isolectin b4
    Fitc Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc isolectin b4/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc isolectin b4 - by Bioz Stars, 2024-06
    86/100 stars

    Images

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 86
    Vector Laboratories isolectin b 4
    Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isolectin b 4/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isolectin b 4 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Vector Laboratories biotin conjugated isolectin b4
    Biotin Conjugated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotin conjugated isolectin b4/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotin conjugated isolectin b4 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Vector Laboratories lectins
    Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    lectins - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Vector Laboratories biotinylated lectins
    <t> Lectins </t> used in this study, and the glycans to which they bind, according to CFG data. <t> Lectins </t> displayed binding to MVs are marked in grey. The three lectins marked with * displayed binding non-specifically to the glass slides, and those lectins along with the lectin marked # were only analyzed using flow cytometry. The structure of glycans was presented in the symbol nomenclature for glycans (SNFGs) format ( https://www.ncbi.nlm.nih.gov/glycans/snfg.html accessed on 26 February 2024) using Carbohydrate Structure Databases, 16 March 2024 found at http://csdb.glycoscience.ru/database/core/wizard.html .
    Biotinylated Lectins, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/biotinylated lectins/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    biotinylated lectins - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Vector Laboratories anti isolectin b4 antibody conjugated to fluorescein
    SKM injection promotes a transient increase in vasculature development. Tissue sections were stained against smooth muscle (ɑSMA, green), endothelial cells <t>(isolectin,</t> cyan), and nuclei (DAPI). Arterioles, double positive for ɑSMA and isolectin, were counted and assessed for lumen area. Representative images of stained tissue from saline (A) and SKM (B) treated animals at 7 days post-injection are shown (Scale bar: 100 µm). (C) At 7 days post-injection, SKM group demonstrated significantly higher vessel density throughout the whole tissue section (unpaired t -test, ***p<0.001). (D) The vessels in SKM injected animals demonstrated a significantly smaller overall size distribution at 7 days post-injection (Mann-Whitney test, ****p<0.0001). (F) At 4 weeks post-injection, there was a nonsignificant increase in vessel density within the scar area in SKM injected animals (unpaired t -test, p=0.079). (F) At 4 weeks post-injection, the smaller size distribution of vessels in the SKM group was maintained (Mann-Whitney test, ***p<0.001). n = 6 animals per group.
    Anti Isolectin B4 Antibody Conjugated To Fluorescein, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti isolectin b4 antibody conjugated to fluorescein/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti isolectin b4 antibody conjugated to fluorescein - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Vector Laboratories fitc conjugated isolectin b4
    SKM injection promotes a transient increase in vasculature development. Tissue sections were stained against smooth muscle (ɑSMA, green), endothelial cells <t>(isolectin,</t> cyan), and nuclei (DAPI). Arterioles, double positive for ɑSMA and isolectin, were counted and assessed for lumen area. Representative images of stained tissue from saline (A) and SKM (B) treated animals at 7 days post-injection are shown (Scale bar: 100 µm). (C) At 7 days post-injection, SKM group demonstrated significantly higher vessel density throughout the whole tissue section (unpaired t -test, ***p<0.001). (D) The vessels in SKM injected animals demonstrated a significantly smaller overall size distribution at 7 days post-injection (Mann-Whitney test, ****p<0.0001). (F) At 4 weeks post-injection, there was a nonsignificant increase in vessel density within the scar area in SKM injected animals (unpaired t -test, p=0.079). (F) At 4 weeks post-injection, the smaller size distribution of vessels in the SKM group was maintained (Mann-Whitney test, ***p<0.001). n = 6 animals per group.
    Fitc Conjugated Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc conjugated isolectin b4/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc conjugated isolectin b4 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    86
    Vector Laboratories fitc isolectin b4
    SKM injection promotes a transient increase in vasculature development. Tissue sections were stained against smooth muscle (ɑSMA, green), endothelial cells <t>(isolectin,</t> cyan), and nuclei (DAPI). Arterioles, double positive for ɑSMA and isolectin, were counted and assessed for lumen area. Representative images of stained tissue from saline (A) and SKM (B) treated animals at 7 days post-injection are shown (Scale bar: 100 µm). (C) At 7 days post-injection, SKM group demonstrated significantly higher vessel density throughout the whole tissue section (unpaired t -test, ***p<0.001). (D) The vessels in SKM injected animals demonstrated a significantly smaller overall size distribution at 7 days post-injection (Mann-Whitney test, ****p<0.0001). (F) At 4 weeks post-injection, there was a nonsignificant increase in vessel density within the scar area in SKM injected animals (unpaired t -test, p=0.079). (F) At 4 weeks post-injection, the smaller size distribution of vessels in the SKM group was maintained (Mann-Whitney test, ***p<0.001). n = 6 animals per group.
    Fitc Isolectin B4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/fitc isolectin b4/product/Vector Laboratories
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    fitc isolectin b4 - by Bioz Stars, 2024-06
    86/100 stars
      Buy from Supplier

    Image Search Results


     Lectins  used in this study, and the glycans to which they bind, according to CFG data.  Lectins  displayed binding to MVs are marked in grey. The three lectins marked with * displayed binding non-specifically to the glass slides, and those lectins along with the lectin marked # were only analyzed using flow cytometry. The structure of glycans was presented in the symbol nomenclature for glycans (SNFGs) format ( https://www.ncbi.nlm.nih.gov/glycans/snfg.html accessed on 26 February 2024) using Carbohydrate Structure Databases, 16 March 2024 found at http://csdb.glycoscience.ru/database/core/wizard.html .

    Journal: International Journal of Molecular Sciences

    Article Title: Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

    doi: 10.3390/ijms25115725

    Figure Lengend Snippet: Lectins used in this study, and the glycans to which they bind, according to CFG data. Lectins displayed binding to MVs are marked in grey. The three lectins marked with * displayed binding non-specifically to the glass slides, and those lectins along with the lectin marked # were only analyzed using flow cytometry. The structure of glycans was presented in the symbol nomenclature for glycans (SNFGs) format ( https://www.ncbi.nlm.nih.gov/glycans/snfg.html accessed on 26 February 2024) using Carbohydrate Structure Databases, 16 March 2024 found at http://csdb.glycoscience.ru/database/core/wizard.html .

    Article Snippet: The biotinylated lectins listed in were obtained from Vector Laboratories (Burlingame, CA, USA).

    Techniques: Binding Assay, Flow Cytometry

    Solid-phase detection of lectin binding to MVs. MVs were derived from EA.hy 926 cells and immobilized on glass slides (solid-phase assay), incubated with biotinylated lectins, and then visualized with Str-Alexa Fluor 555, as described in the Materials and Methods section.

    Journal: International Journal of Molecular Sciences

    Article Title: Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

    doi: 10.3390/ijms25115725

    Figure Lengend Snippet: Solid-phase detection of lectin binding to MVs. MVs were derived from EA.hy 926 cells and immobilized on glass slides (solid-phase assay), incubated with biotinylated lectins, and then visualized with Str-Alexa Fluor 555, as described in the Materials and Methods section.

    Article Snippet: The biotinylated lectins listed in were obtained from Vector Laboratories (Burlingame, CA, USA).

    Techniques: Binding Assay, Derivative Assay, Incubation

    Flow cytometry detection of lectins DSA and SNA binding to glycosidase-treated MVs. MVs were incubated with either β-galactosidase or neuraminidase, washed and incubated with biotinylated lectins, as described in the Materials and Methods section. In the dot plots, forward scatter (FS) was plotted against the logarithm of fluorescence (FL1) and the number given in the rectangles (for the purple dots) indicate the percentage of lectin bound to MVs.

    Journal: International Journal of Molecular Sciences

    Article Title: Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

    doi: 10.3390/ijms25115725

    Figure Lengend Snippet: Flow cytometry detection of lectins DSA and SNA binding to glycosidase-treated MVs. MVs were incubated with either β-galactosidase or neuraminidase, washed and incubated with biotinylated lectins, as described in the Materials and Methods section. In the dot plots, forward scatter (FS) was plotted against the logarithm of fluorescence (FL1) and the number given in the rectangles (for the purple dots) indicate the percentage of lectin bound to MVs.

    Article Snippet: The biotinylated lectins listed in were obtained from Vector Laboratories (Burlingame, CA, USA).

    Techniques: Flow Cytometry, Binding Assay, Incubation, Fluorescence

    Flow cytometry binding of lectins to EA.hy 926 cells. Cells were incubated with biotinylated lectins followed by Str-FITC, as described in the Materials and Methods Section. Results shown include data from four experiments and error bars represent standard deviations. A mean fluorescence <50 was considered as negative.

    Journal: International Journal of Molecular Sciences

    Article Title: Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

    doi: 10.3390/ijms25115725

    Figure Lengend Snippet: Flow cytometry binding of lectins to EA.hy 926 cells. Cells were incubated with biotinylated lectins followed by Str-FITC, as described in the Materials and Methods Section. Results shown include data from four experiments and error bars represent standard deviations. A mean fluorescence <50 was considered as negative.

    Article Snippet: The biotinylated lectins listed in were obtained from Vector Laboratories (Burlingame, CA, USA).

    Techniques: Flow Cytometry, Binding Assay, Incubation, Fluorescence

    Binding of lectins to MVs derived from EA.hy 926 cells treated with inhibitors to N -glycosylation, DMJ ( A ) or O -glycosylation, GalNAcαBn ( B ). MVs were isolated from native cells and cells treated overnight with GalNAcαBn or DMJ. Flow cytometry results shown include data from two experiments and error bars represent standard deviations; *** p < 0.001, ** p < 0.01, * p < 0.1.

    Journal: International Journal of Molecular Sciences

    Article Title: Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

    doi: 10.3390/ijms25115725

    Figure Lengend Snippet: Binding of lectins to MVs derived from EA.hy 926 cells treated with inhibitors to N -glycosylation, DMJ ( A ) or O -glycosylation, GalNAcαBn ( B ). MVs were isolated from native cells and cells treated overnight with GalNAcαBn or DMJ. Flow cytometry results shown include data from two experiments and error bars represent standard deviations; *** p < 0.001, ** p < 0.01, * p < 0.1.

    Article Snippet: The biotinylated lectins listed in were obtained from Vector Laboratories (Burlingame, CA, USA).

    Techniques: Binding Assay, Derivative Assay, Isolation, Flow Cytometry

    Binding of lectins to MVs released from the EA.hy 926 cells treated with enzymes. MVs were isolated from cells treated with collagenase, hyaluronidase, and trypsin. Flow cytometry results include data from three experiments; error bars represent standard deviations; * p < 0.1.

    Journal: International Journal of Molecular Sciences

    Article Title: Surface Glycans of Microvesicles Derived from Endothelial Cells, as Probed Using Plant Lectins

    doi: 10.3390/ijms25115725

    Figure Lengend Snippet: Binding of lectins to MVs released from the EA.hy 926 cells treated with enzymes. MVs were isolated from cells treated with collagenase, hyaluronidase, and trypsin. Flow cytometry results include data from three experiments; error bars represent standard deviations; * p < 0.1.

    Article Snippet: The biotinylated lectins listed in were obtained from Vector Laboratories (Burlingame, CA, USA).

    Techniques: Binding Assay, Isolation, Flow Cytometry

    SKM injection promotes a transient increase in vasculature development. Tissue sections were stained against smooth muscle (ɑSMA, green), endothelial cells (isolectin, cyan), and nuclei (DAPI). Arterioles, double positive for ɑSMA and isolectin, were counted and assessed for lumen area. Representative images of stained tissue from saline (A) and SKM (B) treated animals at 7 days post-injection are shown (Scale bar: 100 µm). (C) At 7 days post-injection, SKM group demonstrated significantly higher vessel density throughout the whole tissue section (unpaired t -test, ***p<0.001). (D) The vessels in SKM injected animals demonstrated a significantly smaller overall size distribution at 7 days post-injection (Mann-Whitney test, ****p<0.0001). (F) At 4 weeks post-injection, there was a nonsignificant increase in vessel density within the scar area in SKM injected animals (unpaired t -test, p=0.079). (F) At 4 weeks post-injection, the smaller size distribution of vessels in the SKM group was maintained (Mann-Whitney test, ***p<0.001). n = 6 animals per group.

    Journal: Bioactive Materials

    Article Title: Immunomodulatory extracellular matrix hydrogel induces tissue regeneration in a model of partial glossectomy

    doi: 10.1016/j.bioactmat.2024.05.001

    Figure Lengend Snippet: SKM injection promotes a transient increase in vasculature development. Tissue sections were stained against smooth muscle (ɑSMA, green), endothelial cells (isolectin, cyan), and nuclei (DAPI). Arterioles, double positive for ɑSMA and isolectin, were counted and assessed for lumen area. Representative images of stained tissue from saline (A) and SKM (B) treated animals at 7 days post-injection are shown (Scale bar: 100 µm). (C) At 7 days post-injection, SKM group demonstrated significantly higher vessel density throughout the whole tissue section (unpaired t -test, ***p<0.001). (D) The vessels in SKM injected animals demonstrated a significantly smaller overall size distribution at 7 days post-injection (Mann-Whitney test, ****p<0.0001). (F) At 4 weeks post-injection, there was a nonsignificant increase in vessel density within the scar area in SKM injected animals (unpaired t -test, p=0.079). (F) At 4 weeks post-injection, the smaller size distribution of vessels in the SKM group was maintained (Mann-Whitney test, ***p<0.001). n = 6 animals per group.

    Article Snippet: For evaluation of vascularization, blood vessels were identified with a co-stain of an anti-ɑSMA antibody (Agilent, Santa Clara, California, 1:75) with an Alexa Fluor™ 568 secondary (Invitrogen, Carlsbad, California, 1:500) and anti-isolectin B4 antibody conjugated to fluorescein (Vector Laboratories, Newark, California, 1:75), alongside an anti-collagen I antibody with an Alexa Fluor™ 647 secondary (Invitrogen, Carlsbad, California, 1:500).

    Techniques: Injection, Staining, Saline, MANN-WHITNEY