gsl i b 4 isolectin conjugated  (Vector Laboratories)


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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    isolectin b 4  (Vector Laboratories)


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    Vector Laboratories isolectin b 4
    Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated isolectin b 4  (Vector Laboratories)


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    Vector Laboratories biotinylated isolectin b 4
    Increased capillary density of cardiac left ventricles of TRPC5 mice subjected to pressure overload. Representative images of capillary density in transverse cardiac left ventricle sections from WT and TRPC5 KO mice subjected to ( A ) 4 weeks ( n = 5–7) and ( B ) 10 weeks ( n = 10–12) of abdominal aortic banding (AAB) or sham. Endothelial cells were stained with <t>Isolectin</t> <t>B</t> <t>4</t> (IB 4 ), and cardiomyocyte plasma membranes with wheat germ agglutinin (WGA). Colour image overlays were created in Adobe Photoshop CC software; IB 4 green and WGA red with overlay in yellow; bar represents 50 µm. ( C ) Quantification was determined as total number of capillaries as a ratio to total number of cardiomyocytes per field of view using ImageJ software (version 1.48). Data shown as mean ± s.e.m. * p < 0.05 as determined by two-ANOVA followed by Bonferroni post-hoc correction.
    Biotinylated Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Transient Receptor Potential Canonical 5 (TRPC5): Regulation of Heart Rate and Protection against Pathological Cardiac Hypertrophy"

    Article Title: Transient Receptor Potential Canonical 5 (TRPC5): Regulation of Heart Rate and Protection against Pathological Cardiac Hypertrophy

    Journal: Biomolecules

    doi: 10.3390/biom14040442

    Increased capillary density of cardiac left ventricles of TRPC5 mice subjected to pressure overload. Representative images of capillary density in transverse cardiac left ventricle sections from WT and TRPC5 KO mice subjected to ( A ) 4 weeks ( n = 5–7) and ( B ) 10 weeks ( n = 10–12) of abdominal aortic banding (AAB) or sham. Endothelial cells were stained with Isolectin B 4 (IB 4 ), and cardiomyocyte plasma membranes with wheat germ agglutinin (WGA). Colour image overlays were created in Adobe Photoshop CC software; IB 4 green and WGA red with overlay in yellow; bar represents 50 µm. ( C ) Quantification was determined as total number of capillaries as a ratio to total number of cardiomyocytes per field of view using ImageJ software (version 1.48). Data shown as mean ± s.e.m. * p < 0.05 as determined by two-ANOVA followed by Bonferroni post-hoc correction.
    Figure Legend Snippet: Increased capillary density of cardiac left ventricles of TRPC5 mice subjected to pressure overload. Representative images of capillary density in transverse cardiac left ventricle sections from WT and TRPC5 KO mice subjected to ( A ) 4 weeks ( n = 5–7) and ( B ) 10 weeks ( n = 10–12) of abdominal aortic banding (AAB) or sham. Endothelial cells were stained with Isolectin B 4 (IB 4 ), and cardiomyocyte plasma membranes with wheat germ agglutinin (WGA). Colour image overlays were created in Adobe Photoshop CC software; IB 4 green and WGA red with overlay in yellow; bar represents 50 µm. ( C ) Quantification was determined as total number of capillaries as a ratio to total number of cardiomyocytes per field of view using ImageJ software (version 1.48). Data shown as mean ± s.e.m. * p < 0.05 as determined by two-ANOVA followed by Bonferroni post-hoc correction.

    Techniques Used: Staining, Software

    dylight 594 labeled gsli isolectin b 4  (Vector Laboratories)


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    Vector Laboratories dylight 594 labeled gsli isolectin b 4
    Dylight 594 Labeled Gsli Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    griffonia simplicolia isolectin b 4 dylight 594 conjugate  (Vector Laboratories)


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    Vector Laboratories griffonia simplicolia isolectin b 4 dylight 594 conjugate
    (A–E) In vivo HLI causes loss of blood perfusion in skeletal muscles distal to the ligation site. Laser Doppler perfusion imaging (A) and quantification (B) of the paw in the prone position at various time points following induction of HLI. (C) Representative immunofluorescent cross-sectional images of control and ischemic EDL and FDB muscles at 48 h post-HLI. Sections were probed for <t>GS-IB</t> <t>4</t> lectin (orange) to label actively perfused vessels, CD31 (green) to label total endothelial cells, and dystrophin (red) to outline the sarcolemma. Loss of blood perfusion to the EDL and FDB was confirmed by measuring the area (D) and fluorescence (E) of GS-IB 4 + vessels alongside plotting the signal ratio of GS-IB 4 to CD31 + (F). * P < 0.0005 compared to contralateral control.
    Griffonia Simplicolia Isolectin B 4 Dylight 594 Conjugate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Hypoxia Resistance Is an Inherent Phenotype of the Mouse Flexor Digitorum Brevis Skeletal Muscle"

    Article Title: Hypoxia Resistance Is an Inherent Phenotype of the Mouse Flexor Digitorum Brevis Skeletal Muscle

    Journal: Function

    doi: 10.1093/function/zqad012

    (A–E) In vivo HLI causes loss of blood perfusion in skeletal muscles distal to the ligation site. Laser Doppler perfusion imaging (A) and quantification (B) of the paw in the prone position at various time points following induction of HLI. (C) Representative immunofluorescent cross-sectional images of control and ischemic EDL and FDB muscles at 48 h post-HLI. Sections were probed for GS-IB 4 lectin (orange) to label actively perfused vessels, CD31 (green) to label total endothelial cells, and dystrophin (red) to outline the sarcolemma. Loss of blood perfusion to the EDL and FDB was confirmed by measuring the area (D) and fluorescence (E) of GS-IB 4 + vessels alongside plotting the signal ratio of GS-IB 4 to CD31 + (F). * P < 0.0005 compared to contralateral control.
    Figure Legend Snippet: (A–E) In vivo HLI causes loss of blood perfusion in skeletal muscles distal to the ligation site. Laser Doppler perfusion imaging (A) and quantification (B) of the paw in the prone position at various time points following induction of HLI. (C) Representative immunofluorescent cross-sectional images of control and ischemic EDL and FDB muscles at 48 h post-HLI. Sections were probed for GS-IB 4 lectin (orange) to label actively perfused vessels, CD31 (green) to label total endothelial cells, and dystrophin (red) to outline the sarcolemma. Loss of blood perfusion to the EDL and FDB was confirmed by measuring the area (D) and fluorescence (E) of GS-IB 4 + vessels alongside plotting the signal ratio of GS-IB 4 to CD31 + (F). * P < 0.0005 compared to contralateral control.

    Techniques Used: In Vivo, Ligation, Imaging, Fluorescence

    gsl i b 4 isolectin conjugated  (Vector Laboratories)


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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotin conjugated griffonia simplicifolia lectin gsl i isolectin b 4  (Vector Laboratories)


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    Vector Laboratories biotin conjugated griffonia simplicifolia lectin gsl i isolectin b 4
    Biotin Conjugated Griffonia Simplicifolia Lectin Gsl I Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    biotinylated isolectin b 4  (Vector Laboratories)


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    Vector Laboratories biotinylated isolectin b 4
    Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and <t>IB</t> <t>4</t> positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).
    Biotinylated Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Intrinsic Up-Regulation of 2-AG Favors an Area Specific Neuronal Survival in Different In Vitro Models of Neuronal Damage"

    Article Title: Intrinsic Up-Regulation of 2-AG Favors an Area Specific Neuronal Survival in Different In Vitro Models of Neuronal Damage

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0051208

    Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and IB 4 positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).
    Figure Legend Snippet: Images show the molecular layer (ml), the granular cell layer (gcl) and part of the hilus (hi) region. The enzymes DAGL and MAGL are given in green, GFAP in red and IB 4 positive microglial cells are displayed in blue. The asterisks indicate NeuN co-localized DAGL and MAGL staining in the gcl (NeuN data not shown). DAGL was co-localized with GFAP positive cells only after PPT and NMDA excitotoxic lesion but not in control OHSC (big arrows). IB 4 positive microglial cells were not DAGL immunoreactive under control conditions or after neuronal damage (small arrows). After PPT the DAGL was seen in the outer half of the gcl whereas the inner half of the granular cell layer was not labeled (24 hpl, asterisk). GFAP positive cells expressed a strong co-localization with the MAGL protein in control OHSC that disappeared 24 h after PPT and in NMDA lesioned OHSC (big arrows). A weak MAGL immunoreaction was found in vesicular structures in microglial cells presumably representing phagocytosis bodies in their cytoplasms (small arrows). (Bar = 50 µm).

    Techniques Used: Staining, Labeling

    biotinylated bandeiraea simplicifolia i bs i isolectin b 4  (Vector Laboratories)


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    Vector Laboratories biotinylated bandeiraea simplicifolia i bs i isolectin b 4
    Biotinylated Bandeiraea Simplicifolia I Bs I Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    isolectin b 4  (Vector Laboratories)


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    Vector Laboratories isolectin b 4
    (D) Quantification revealed significantly greater numbers of <t>IB</t> <t>4</t> + axons in normal nerves compared to acellular grafts (*) and in NT3 grafts compared to all other experimental groups (**). (E) The number of CGRP + axons was not significantly different between experimental groups. Values represent M ± SEM of n = 3; p<0.05. Further details on statistical analysis provided as . Scale bar for A–C: 100 µm.
    Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Immunohistochemical, Ultrastructural and Functional Analysis of Axonal Regeneration through Peripheral Nerve Grafts Containing Schwann Cells Expressing BDNF, CNTF or NT3"

    Article Title: Immunohistochemical, Ultrastructural and Functional Analysis of Axonal Regeneration through Peripheral Nerve Grafts Containing Schwann Cells Expressing BDNF, CNTF or NT3

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0069987

    (D) Quantification revealed significantly greater numbers of IB 4 + axons in normal nerves compared to acellular grafts (*) and in NT3 grafts compared to all other experimental groups (**). (E) The number of CGRP + axons was not significantly different between experimental groups. Values represent M ± SEM of n = 3; p<0.05. Further details on statistical analysis provided as . Scale bar for A–C: 100 µm.
    Figure Legend Snippet: (D) Quantification revealed significantly greater numbers of IB 4 + axons in normal nerves compared to acellular grafts (*) and in NT3 grafts compared to all other experimental groups (**). (E) The number of CGRP + axons was not significantly different between experimental groups. Values represent M ± SEM of n = 3; p<0.05. Further details on statistical analysis provided as . Scale bar for A–C: 100 µm.

    Techniques Used:

    bsi b 4 conjugated to dylight 594  (Vector Laboratories)


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    Vector Laboratories bsi b 4 conjugated to dylight 594
    No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in <xref ref-type= Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only." width="250" height="auto" />
    Bsi B 4 Conjugated To Dylight 594, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria"

    Article Title: A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2022.958952

    No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in <xref ref-type= Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only." title="No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B" property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only.

    Techniques Used: Binding Assay, Cell Culture, Staining, Dot Blot, Positive Control, Derivative Assay, SYBR Green Assay

    Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood from a type B blood donor and α-Gal-MSA (positive control) by 27H8, M86 or Lectin (BSI-B 4 ). (B) Screening of 27H8 subcloned hybridoma SN (upper row) and purified 27H8 antibody (middle row) on lysed kidney tissue or cultured kidney cells of wildtype (WT) and GGTA1 knockout (KO) pigs and on WT kidney tissue samples digested with α-Galactosidase (Dig. WT). Further screening molecules: aminopeptidase N (APN) glycosylated with α-Gal, APN only and (α-Gal-containing) bovine thyroglobulin. (A, B) Samples in a row were blotted on one membrane. See <xref ref-type= Supplementary Figure 1 for uncropped blots. (C) Immunofluorescence microscopy of pig kidney tissue specimens (WT and GGTA1 KO) stained with 27H8 (red) and M86 (green) in the glomerulus region. IgG1 isotype ctrl and secondary antibody only stains (anti-IgG1/anti-IgM) served as controls for fluorescence signal. DNA stained with DAPI (blue). Scale bar (white, left corner): 124.4 µm. (D) Flow cytometry analysis of human embryonic kidney (HEK) cells expressing α-Gal glycosylated APN (upper panel) and APN only (lower panel) stained with 27H8 (red), M86 (green) and BSI-B 4 (blue). Controls: unstained (grey) and Isotype controls (IgG1, IgM, both in black). (A–D) If not otherwise indicated, 27H8 was applied in the purified version." title="Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood" property="contentUrl" width="100%" height="100%"/>
    Figure Legend Snippet: Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood from a type B blood donor and α-Gal-MSA (positive control) by 27H8, M86 or Lectin (BSI-B 4 ). (B) Screening of 27H8 subcloned hybridoma SN (upper row) and purified 27H8 antibody (middle row) on lysed kidney tissue or cultured kidney cells of wildtype (WT) and GGTA1 knockout (KO) pigs and on WT kidney tissue samples digested with α-Galactosidase (Dig. WT). Further screening molecules: aminopeptidase N (APN) glycosylated with α-Gal, APN only and (α-Gal-containing) bovine thyroglobulin. (A, B) Samples in a row were blotted on one membrane. See Supplementary Figure 1 for uncropped blots. (C) Immunofluorescence microscopy of pig kidney tissue specimens (WT and GGTA1 KO) stained with 27H8 (red) and M86 (green) in the glomerulus region. IgG1 isotype ctrl and secondary antibody only stains (anti-IgG1/anti-IgM) served as controls for fluorescence signal. DNA stained with DAPI (blue). Scale bar (white, left corner): 124.4 µm. (D) Flow cytometry analysis of human embryonic kidney (HEK) cells expressing α-Gal glycosylated APN (upper panel) and APN only (lower panel) stained with 27H8 (red), M86 (green) and BSI-B 4 (blue). Controls: unstained (grey) and Isotype controls (IgG1, IgM, both in black). (A–D) If not otherwise indicated, 27H8 was applied in the purified version.

    Techniques Used: Dot Blot, Staining, Positive Control, Purification, Cell Culture, Knock-Out, Immunofluorescence, Microscopy, Fluorescence, Flow Cytometry, Expressing

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    Vector Laboratories gsl i b 4 isolectin conjugated
    Gsl I B 4 Isolectin Conjugated, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories isolectin b 4
    Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotinylated isolectin b 4
    Increased capillary density of cardiac left ventricles of TRPC5 mice subjected to pressure overload. Representative images of capillary density in transverse cardiac left ventricle sections from WT and TRPC5 KO mice subjected to ( A ) 4 weeks ( n = 5–7) and ( B ) 10 weeks ( n = 10–12) of abdominal aortic banding (AAB) or sham. Endothelial cells were stained with <t>Isolectin</t> <t>B</t> <t>4</t> (IB 4 ), and cardiomyocyte plasma membranes with wheat germ agglutinin (WGA). Colour image overlays were created in Adobe Photoshop CC software; IB 4 green and WGA red with overlay in yellow; bar represents 50 µm. ( C ) Quantification was determined as total number of capillaries as a ratio to total number of cardiomyocytes per field of view using ImageJ software (version 1.48). Data shown as mean ± s.e.m. * p < 0.05 as determined by two-ANOVA followed by Bonferroni post-hoc correction.
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    Vector Laboratories dylight 594 labeled gsli isolectin b 4
    Increased capillary density of cardiac left ventricles of TRPC5 mice subjected to pressure overload. Representative images of capillary density in transverse cardiac left ventricle sections from WT and TRPC5 KO mice subjected to ( A ) 4 weeks ( n = 5–7) and ( B ) 10 weeks ( n = 10–12) of abdominal aortic banding (AAB) or sham. Endothelial cells were stained with <t>Isolectin</t> <t>B</t> <t>4</t> (IB 4 ), and cardiomyocyte plasma membranes with wheat germ agglutinin (WGA). Colour image overlays were created in Adobe Photoshop CC software; IB 4 green and WGA red with overlay in yellow; bar represents 50 µm. ( C ) Quantification was determined as total number of capillaries as a ratio to total number of cardiomyocytes per field of view using ImageJ software (version 1.48). Data shown as mean ± s.e.m. * p < 0.05 as determined by two-ANOVA followed by Bonferroni post-hoc correction.
    Dylight 594 Labeled Gsli Isolectin B 4, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories griffonia simplicolia isolectin b 4 dylight 594 conjugate
    (A–E) In vivo HLI causes loss of blood perfusion in skeletal muscles distal to the ligation site. Laser Doppler perfusion imaging (A) and quantification (B) of the paw in the prone position at various time points following induction of HLI. (C) Representative immunofluorescent cross-sectional images of control and ischemic EDL and FDB muscles at 48 h post-HLI. Sections were probed for <t>GS-IB</t> <t>4</t> lectin (orange) to label actively perfused vessels, CD31 (green) to label total endothelial cells, and dystrophin (red) to outline the sarcolemma. Loss of blood perfusion to the EDL and FDB was confirmed by measuring the area (D) and fluorescence (E) of GS-IB 4 + vessels alongside plotting the signal ratio of GS-IB 4 to CD31 + (F). * P < 0.0005 compared to contralateral control.
    Griffonia Simplicolia Isolectin B 4 Dylight 594 Conjugate, supplied by Vector Laboratories, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Vector Laboratories biotin conjugated griffonia simplicifolia lectin gsl i isolectin b 4
    (A–E) In vivo HLI causes loss of blood perfusion in skeletal muscles distal to the ligation site. Laser Doppler perfusion imaging (A) and quantification (B) of the paw in the prone position at various time points following induction of HLI. (C) Representative immunofluorescent cross-sectional images of control and ischemic EDL and FDB muscles at 48 h post-HLI. Sections were probed for <t>GS-IB</t> <t>4</t> lectin (orange) to label actively perfused vessels, CD31 (green) to label total endothelial cells, and dystrophin (red) to outline the sarcolemma. Loss of blood perfusion to the EDL and FDB was confirmed by measuring the area (D) and fluorescence (E) of GS-IB 4 + vessels alongside plotting the signal ratio of GS-IB 4 to CD31 + (F). * P < 0.0005 compared to contralateral control.
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    (A–E) In vivo HLI causes loss of blood perfusion in skeletal muscles distal to the ligation site. Laser Doppler perfusion imaging (A) and quantification (B) of the paw in the prone position at various time points following induction of HLI. (C) Representative immunofluorescent cross-sectional images of control and ischemic EDL and FDB muscles at 48 h post-HLI. Sections were probed for <t>GS-IB</t> <t>4</t> lectin (orange) to label actively perfused vessels, CD31 (green) to label total endothelial cells, and dystrophin (red) to outline the sarcolemma. Loss of blood perfusion to the EDL and FDB was confirmed by measuring the area (D) and fluorescence (E) of GS-IB 4 + vessels alongside plotting the signal ratio of GS-IB 4 to CD31 + (F). * P < 0.0005 compared to contralateral control.
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    Vector Laboratories bsi b 4 conjugated to dylight 594
    No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in <xref ref-type= Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only." width="250" height="auto" />
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    Image Search Results


    Increased capillary density of cardiac left ventricles of TRPC5 mice subjected to pressure overload. Representative images of capillary density in transverse cardiac left ventricle sections from WT and TRPC5 KO mice subjected to ( A ) 4 weeks ( n = 5–7) and ( B ) 10 weeks ( n = 10–12) of abdominal aortic banding (AAB) or sham. Endothelial cells were stained with Isolectin B 4 (IB 4 ), and cardiomyocyte plasma membranes with wheat germ agglutinin (WGA). Colour image overlays were created in Adobe Photoshop CC software; IB 4 green and WGA red with overlay in yellow; bar represents 50 µm. ( C ) Quantification was determined as total number of capillaries as a ratio to total number of cardiomyocytes per field of view using ImageJ software (version 1.48). Data shown as mean ± s.e.m. * p < 0.05 as determined by two-ANOVA followed by Bonferroni post-hoc correction.

    Journal: Biomolecules

    Article Title: Transient Receptor Potential Canonical 5 (TRPC5): Regulation of Heart Rate and Protection against Pathological Cardiac Hypertrophy

    doi: 10.3390/biom14040442

    Figure Lengend Snippet: Increased capillary density of cardiac left ventricles of TRPC5 mice subjected to pressure overload. Representative images of capillary density in transverse cardiac left ventricle sections from WT and TRPC5 KO mice subjected to ( A ) 4 weeks ( n = 5–7) and ( B ) 10 weeks ( n = 10–12) of abdominal aortic banding (AAB) or sham. Endothelial cells were stained with Isolectin B 4 (IB 4 ), and cardiomyocyte plasma membranes with wheat germ agglutinin (WGA). Colour image overlays were created in Adobe Photoshop CC software; IB 4 green and WGA red with overlay in yellow; bar represents 50 µm. ( C ) Quantification was determined as total number of capillaries as a ratio to total number of cardiomyocytes per field of view using ImageJ software (version 1.48). Data shown as mean ± s.e.m. * p < 0.05 as determined by two-ANOVA followed by Bonferroni post-hoc correction.

    Article Snippet: Endothelial cells were stained with biotinylated Isolectin B 4 (IB 4 , 1-in-100 dilution, B-1205, Vector Laboratories) and DyLight 488 streptavidin (1-in-200 dilution SA-5488, Vector Laboratories), to outline capillaries.

    Techniques: Staining, Software

    (A–E) In vivo HLI causes loss of blood perfusion in skeletal muscles distal to the ligation site. Laser Doppler perfusion imaging (A) and quantification (B) of the paw in the prone position at various time points following induction of HLI. (C) Representative immunofluorescent cross-sectional images of control and ischemic EDL and FDB muscles at 48 h post-HLI. Sections were probed for GS-IB 4 lectin (orange) to label actively perfused vessels, CD31 (green) to label total endothelial cells, and dystrophin (red) to outline the sarcolemma. Loss of blood perfusion to the EDL and FDB was confirmed by measuring the area (D) and fluorescence (E) of GS-IB 4 + vessels alongside plotting the signal ratio of GS-IB 4 to CD31 + (F). * P < 0.0005 compared to contralateral control.

    Journal: Function

    Article Title: Hypoxia Resistance Is an Inherent Phenotype of the Mouse Flexor Digitorum Brevis Skeletal Muscle

    doi: 10.1093/function/zqad012

    Figure Lengend Snippet: (A–E) In vivo HLI causes loss of blood perfusion in skeletal muscles distal to the ligation site. Laser Doppler perfusion imaging (A) and quantification (B) of the paw in the prone position at various time points following induction of HLI. (C) Representative immunofluorescent cross-sectional images of control and ischemic EDL and FDB muscles at 48 h post-HLI. Sections were probed for GS-IB 4 lectin (orange) to label actively perfused vessels, CD31 (green) to label total endothelial cells, and dystrophin (red) to outline the sarcolemma. Loss of blood perfusion to the EDL and FDB was confirmed by measuring the area (D) and fluorescence (E) of GS-IB 4 + vessels alongside plotting the signal ratio of GS-IB 4 to CD31 + (F). * P < 0.0005 compared to contralateral control.

    Article Snippet: Briefly, mice were administered a retro-orbital injection of Griffonia simplicolia isolectin-B 4 Dylight 594 conjugate (Vector Labs, Burlingame, CA) (50 µL of 1 mg/mL) immediately following the induction of HLI.

    Techniques: In Vivo, Ligation, Imaging, Fluorescence

    No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in <xref ref-type= Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only." width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria

    doi: 10.3389/fimmu.2022.958952

    Figure Lengend Snippet: No binding of 27H8 and M86 to intestinal bacteria in contrast to BSI-B 4. (A) Histograms of flow cytometric analysis of cultured bacterial strains stained with 27H8, M86 and BSI-B 4 and respective isotype controls (IgG1 and IgM). Strains: E coli O86:B7, E.coli BL21, E coli Human Species (HS) and Lactobacillus rhamnosus ( L. rhamnosus ). (B) Dot blot stain of lysed E coli O86:B7 and α-Gal-MSA as positive control. Uncropped blots depicted in Supplementary Figure 1E . (C) Representative histogram blots of flow cytometric analysis of intestinal content (derived from small intestine, cecum and colon) from Ggta1 KO mice (n=3) stained with biotinylated 27H8 and BSI-B 4 , pre-gated for SYBR green positive bacteria ( Supplementary Figure 2A ). (D) Live splenocytes of Ggta1 KO and WT mouse ( Supplementary Figure 2B ) stained with biotinylated 27H8 and BSI-B 4 . (C, D) Control staining with IgG1-biotin isotype and streptavidin-PE (SAv-PE) only.

    Article Snippet: 5x10 5 cells were seeded and stained with either 27H8 purified antibody or IgG1κ isotype control (clone: B3102E8, Southern Biotech) at 1 µg/ml, a 1:10 dilution of M86 supernatant, a 1:10 dilution (40 µg/ml) of mouse IgM isotype control (clone: MOPC 104E, Sigma) or a 1:100 dilution of BSI-B 4 conjugated to DyLight ® 594 ( Griffonia simplicifolia isolectin B4, Vector Laboratories, Burlingame, CA, USA) in BSA-buffer.

    Techniques: Binding Assay, Cell Culture, Staining, Dot Blot, Positive Control, Derivative Assay, SYBR Green Assay

    Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood from a type B blood donor and α-Gal-MSA (positive control) by 27H8, M86 or Lectin (BSI-B 4 ). (B) Screening of 27H8 subcloned hybridoma SN (upper row) and purified 27H8 antibody (middle row) on lysed kidney tissue or cultured kidney cells of wildtype (WT) and GGTA1 knockout (KO) pigs and on WT kidney tissue samples digested with α-Galactosidase (Dig. WT). Further screening molecules: aminopeptidase N (APN) glycosylated with α-Gal, APN only and (α-Gal-containing) bovine thyroglobulin. (A, B) Samples in a row were blotted on one membrane. See <xref ref-type= Supplementary Figure 1 for uncropped blots. (C) Immunofluorescence microscopy of pig kidney tissue specimens (WT and GGTA1 KO) stained with 27H8 (red) and M86 (green) in the glomerulus region. IgG1 isotype ctrl and secondary antibody only stains (anti-IgG1/anti-IgM) served as controls for fluorescence signal. DNA stained with DAPI (blue). Scale bar (white, left corner): 124.4 µm. (D) Flow cytometry analysis of human embryonic kidney (HEK) cells expressing α-Gal glycosylated APN (upper panel) and APN only (lower panel) stained with 27H8 (red), M86 (green) and BSI-B 4 (blue). Controls: unstained (grey) and Isotype controls (IgG1, IgM, both in black). (A–D) If not otherwise indicated, 27H8 was applied in the purified version." width="100%" height="100%">

    Journal: Frontiers in Immunology

    Article Title: A novel monoclonal IgG1 antibody specific for Galactose-alpha-1,3-galactose questions alpha-Gal epitope expression by bacteria

    doi: 10.3389/fimmu.2022.958952

    Figure Lengend Snippet: Specificity of 27H8 monoclonal antibody. (A) Dot blot stain of lysed whole blood from a type B blood donor and α-Gal-MSA (positive control) by 27H8, M86 or Lectin (BSI-B 4 ). (B) Screening of 27H8 subcloned hybridoma SN (upper row) and purified 27H8 antibody (middle row) on lysed kidney tissue or cultured kidney cells of wildtype (WT) and GGTA1 knockout (KO) pigs and on WT kidney tissue samples digested with α-Galactosidase (Dig. WT). Further screening molecules: aminopeptidase N (APN) glycosylated with α-Gal, APN only and (α-Gal-containing) bovine thyroglobulin. (A, B) Samples in a row were blotted on one membrane. See Supplementary Figure 1 for uncropped blots. (C) Immunofluorescence microscopy of pig kidney tissue specimens (WT and GGTA1 KO) stained with 27H8 (red) and M86 (green) in the glomerulus region. IgG1 isotype ctrl and secondary antibody only stains (anti-IgG1/anti-IgM) served as controls for fluorescence signal. DNA stained with DAPI (blue). Scale bar (white, left corner): 124.4 µm. (D) Flow cytometry analysis of human embryonic kidney (HEK) cells expressing α-Gal glycosylated APN (upper panel) and APN only (lower panel) stained with 27H8 (red), M86 (green) and BSI-B 4 (blue). Controls: unstained (grey) and Isotype controls (IgG1, IgM, both in black). (A–D) If not otherwise indicated, 27H8 was applied in the purified version.

    Article Snippet: 5x10 5 cells were seeded and stained with either 27H8 purified antibody or IgG1κ isotype control (clone: B3102E8, Southern Biotech) at 1 µg/ml, a 1:10 dilution of M86 supernatant, a 1:10 dilution (40 µg/ml) of mouse IgM isotype control (clone: MOPC 104E, Sigma) or a 1:100 dilution of BSI-B 4 conjugated to DyLight ® 594 ( Griffonia simplicifolia isolectin B4, Vector Laboratories, Burlingame, CA, USA) in BSA-buffer.

    Techniques: Dot Blot, Staining, Positive Control, Purification, Cell Culture, Knock-Out, Immunofluorescence, Microscopy, Fluorescence, Flow Cytometry, Expressing