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David Kopf Instruments isoflurane
Isoflurane, supplied by David Kopf Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/isoflurane/product/David Kopf Instruments
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
isoflurane - by Bioz Stars, 2021-04
86/100 stars

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Mouse Assay:

Article Title: Single-Cell Analysis of Neuroinflammatory Responses Following Intracranial Injection of G-Deleted Rabies Viruses
Article Snippet: Unpseudotyped rabies virus titers were estimated based on a serial dilution method counting infected HEK 293T cells and quantified as infectious units per ml (IU/ml). .. Stereotaxic Surgeries Mice were initially anesthetized with 5% isoflurane (80% oxygen) and maintained at 1–2.5% isoflurane after placement on the stereotaxic frame (David Kopf Instruments, Model 1900 Stereotaxic Alignment System). ..

Article Title: Rapid increases in immature synapses parallel estrogen-induced hippocampal learning enhancements
Article Snippet: .. Briefly, mice were anesthetized (isoflurane), placed in a stereotaxic frame using atraumatic ear bars (David Kopf instruments), subcutaneously injected with an analgesic and anti-inflammatory (carprofen 50 mg/kg; Rimadyl, Pfizer Canada). .. Ovaries were removed from the dorsal side, local anesthetic was dripped onto the incision (mixture of bupivacaine 0.17%; Hospira; and lidocaine 0.67%; Alveda Pharmaceuticals), then closed with a surgical clip (MikRon Autoclip 9-mm wound clips, MikRon Precision).

Article Title: High and asymmetric somato-dendritic coupling of V1 layer 5 neurons independent of visual stimulation and locomotion
Article Snippet: The scalp was then sutured (Ethicon, Ethilon polyamide size 6) and the animal monitored during recovery in a heated cage before returning to its home cage for 2–3 weeks. .. Headplate and imaging window Under anaesthesia (isoflurane), mice were shaved and mounted onto a stereotaxic frame (David Kopf instruments, CA, USA). ..

Injection:

Article Title: Rapid increases in immature synapses parallel estrogen-induced hippocampal learning enhancements
Article Snippet: .. Briefly, mice were anesthetized (isoflurane), placed in a stereotaxic frame using atraumatic ear bars (David Kopf instruments), subcutaneously injected with an analgesic and anti-inflammatory (carprofen 50 mg/kg; Rimadyl, Pfizer Canada). .. Ovaries were removed from the dorsal side, local anesthetic was dripped onto the incision (mixture of bupivacaine 0.17%; Hospira; and lidocaine 0.67%; Alveda Pharmaceuticals), then closed with a surgical clip (MikRon Autoclip 9-mm wound clips, MikRon Precision).

Imaging:

Article Title: High and asymmetric somato-dendritic coupling of V1 layer 5 neurons independent of visual stimulation and locomotion
Article Snippet: The scalp was then sutured (Ethicon, Ethilon polyamide size 6) and the animal monitored during recovery in a heated cage before returning to its home cage for 2–3 weeks. .. Headplate and imaging window Under anaesthesia (isoflurane), mice were shaved and mounted onto a stereotaxic frame (David Kopf instruments, CA, USA). ..

other:

Article Title: The role of central amygdala corticotropin-releasing factor in predator odor stress-induced avoidance behavior and escalated alcohol drinking in rats.
Article Snippet: 6 2.1.5 Stereotaxic surgeries Rats were anesthetized with 4% isoflurane and anesthesia was maintained with 1-3% isoflurane on the stereotaxic frame (Kopf Instruments, Tujunga, CA).

Article Title: Sex-Dependent Pathology in the HPA Axis at a Sub-acute Period After Experimental Traumatic Brain Injury
Article Snippet: Briefly, rats were anesthetized with 5% isoflurane in 100% O2, heads were shaved, rats were weighed, and placed into a stereotaxic frame (Kopf Instruments, Tujunga, CA) with a nosecone that maintained 2.5% isoflurane for the duration of the procedure.

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    David Kopf Instruments isoflurane
    Overview of the experimental procedures for CSD induction, recording and cortical tissue isolation. CSD induction: CSD events were induced under <t>isoflurane</t> anaesthesia by 30-s application of 300 mM KCl (‘CSD-treated’; or 300 mM NaCl for ‘Sham-treated’ groups) on a craniotomy window overlaying the occipital cortex while DC-potential recordings were made from a glass electrode in the frontal cortex. CSD recording: induction of seven CSD waves was obtained by seven applications of KCl, each eliciting a single CSD event in both WT and FHM1 R192Q mutant mice, at 5-min intervals. Sham surgery with application of 300 mM NaCl did not elicit CSD events. Tissue isolation 24 h post-CSD: 24 h after the end of the CSD or Sham procedure brain tissue was isolated. The middle part of the right-sided cortex (including parietal cortex), through which the CSD waves travelled (hashed area), was dissected for RNA profiling
    Isoflurane, supplied by David Kopf Instruments, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isoflurane/product/David Kopf Instruments
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    isoflurane - by Bioz Stars, 2021-04
    86/100 stars
      Buy from Supplier

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    Overview of the experimental procedures for CSD induction, recording and cortical tissue isolation. CSD induction: CSD events were induced under isoflurane anaesthesia by 30-s application of 300 mM KCl (‘CSD-treated’; or 300 mM NaCl for ‘Sham-treated’ groups) on a craniotomy window overlaying the occipital cortex while DC-potential recordings were made from a glass electrode in the frontal cortex. CSD recording: induction of seven CSD waves was obtained by seven applications of KCl, each eliciting a single CSD event in both WT and FHM1 R192Q mutant mice, at 5-min intervals. Sham surgery with application of 300 mM NaCl did not elicit CSD events. Tissue isolation 24 h post-CSD: 24 h after the end of the CSD or Sham procedure brain tissue was isolated. The middle part of the right-sided cortex (including parietal cortex), through which the CSD waves travelled (hashed area), was dissected for RNA profiling

    Journal: Molecular Neurobiology

    Article Title: Cortical Spreading Depression Causes Unique Dysregulation of Inflammatory Pathways in a Transgenic Mouse Model of Migraine

    doi: 10.1007/s12035-015-9681-5

    Figure Lengend Snippet: Overview of the experimental procedures for CSD induction, recording and cortical tissue isolation. CSD induction: CSD events were induced under isoflurane anaesthesia by 30-s application of 300 mM KCl (‘CSD-treated’; or 300 mM NaCl for ‘Sham-treated’ groups) on a craniotomy window overlaying the occipital cortex while DC-potential recordings were made from a glass electrode in the frontal cortex. CSD recording: induction of seven CSD waves was obtained by seven applications of KCl, each eliciting a single CSD event in both WT and FHM1 R192Q mutant mice, at 5-min intervals. Sham surgery with application of 300 mM NaCl did not elicit CSD events. Tissue isolation 24 h post-CSD: 24 h after the end of the CSD or Sham procedure brain tissue was isolated. The middle part of the right-sided cortex (including parietal cortex), through which the CSD waves travelled (hashed area), was dissected for RNA profiling

    Article Snippet: Induction of CSD and Tissue Isolation Mice were anaesthetized using 1.5 % isoflurane in pressurised air (20 % O2 and 80 % N2 ) and placed onto a stereotactic frame (David Kopf Instruments, Tujunga, CA, USA).

    Techniques: Isolation, Mutagenesis, Mouse Assay

    Activation of the LH GABA -TRN circuits induced cortical arousal and emergence from anesthesia a , Shematic representation of the in vivo polysomnographic recording set up in anesthetic chamber. b , Higher time resolution of of EEG traces shown in (c) depicted by the boxes (1) for control condition and (2) for ChETA-EYFP positive animals after 5 sec light stimulation of the TRN. These illustrate changes at the level of cortical EEG of burst-suppression ration after stimulation during anesthesia (1–1.2 % isoflurane). c , Representative EEG/EMG recordings and heat map EEG power spectrum before, during and after single 5-s optical stimulation (horizontal blue bars) in EYFP ( left ) and ChETA-YFP ( right ) animals during deep anaesthesia. Note the muscle tone that occured in ChETA-YFP (2 out of 5 animals). d , Mean duration ± S.E.M of burst total time ( e ), burst suppression ratio and ( f ), and burst total time duration, before and after bilateral optical stimulation delivered during the EEG burst in control (white) N = and ChETA-EYFP (black), ( N = 4 mice, 3 repetitions per animal). f , Mean duration ± S.E.M of burst total time before and after bilateral optical stimulation delivered during isoelectric period in control (white) and ChETA-EYFP (black) ( N = 4 mice, 3 repetitions per animal). ***, P

    Journal: Nature neuroscience

    Article Title: Hypothalamic feed-forward inhibition of thalamocortical network controls arousal and consciousness

    doi: 10.1038/nn.4209

    Figure Lengend Snippet: Activation of the LH GABA -TRN circuits induced cortical arousal and emergence from anesthesia a , Shematic representation of the in vivo polysomnographic recording set up in anesthetic chamber. b , Higher time resolution of of EEG traces shown in (c) depicted by the boxes (1) for control condition and (2) for ChETA-EYFP positive animals after 5 sec light stimulation of the TRN. These illustrate changes at the level of cortical EEG of burst-suppression ration after stimulation during anesthesia (1–1.2 % isoflurane). c , Representative EEG/EMG recordings and heat map EEG power spectrum before, during and after single 5-s optical stimulation (horizontal blue bars) in EYFP ( left ) and ChETA-YFP ( right ) animals during deep anaesthesia. Note the muscle tone that occured in ChETA-YFP (2 out of 5 animals). d , Mean duration ± S.E.M of burst total time ( e ), burst suppression ratio and ( f ), and burst total time duration, before and after bilateral optical stimulation delivered during the EEG burst in control (white) N = and ChETA-EYFP (black), ( N = 4 mice, 3 repetitions per animal). f , Mean duration ± S.E.M of burst total time before and after bilateral optical stimulation delivered during isoelectric period in control (white) and ChETA-EYFP (black) ( N = 4 mice, 3 repetitions per animal). ***, P

    Article Snippet: Plasmid and viral targeting 6-weeks old Tg(VGAT)::IRES-Cre male or female mice were anesthetized with isoflurane (5% induction, 1.2–1.5% maintenance) and then placed on a small animal digital stereotaxic frame (David Kopf Instruments).

    Techniques: Activation Assay, In Vivo, Size-exclusion Chromatography, Mouse Assay

    SOM + interneurons are active under light anesthesia. A , Left, Two-photon images of two FOVs from SOM-cre::LSL-tdTomato mice bolus loaded with Oregon Green Bapta-1 AM (OGB-1) in upper layer 2/3 of V1 at 950 nm excitation. Middle, Same FOVs at 1040 nm excitation showing SOM + cells expressing tdTomato. Right, Merged images of the two. Scale bar, 50 μm. B , Ca 2+ responses of five SOM + cells from two mice measured under light isoflurane anesthesia showing active SOM + cells. C , Averaged visual-evoked responses of five SOM + cells shown in A (420 ms stimulus marked with a shaded gray box, averaged over eight directions, eight trials each; mean ± SEM). D , Polar plots of the normalized tuning curves for five SOM + cells shown in A and B (each point represents the mean response over eight trials normalized by the maximum response).

    Journal: The Journal of Neuroscience

    Article Title: Cortical Control of Spatial Resolution by VIP+ Interneurons

    doi: 10.1523/JNEUROSCI.1920-16.2016

    Figure Lengend Snippet: SOM + interneurons are active under light anesthesia. A , Left, Two-photon images of two FOVs from SOM-cre::LSL-tdTomato mice bolus loaded with Oregon Green Bapta-1 AM (OGB-1) in upper layer 2/3 of V1 at 950 nm excitation. Middle, Same FOVs at 1040 nm excitation showing SOM + cells expressing tdTomato. Right, Merged images of the two. Scale bar, 50 μm. B , Ca 2+ responses of five SOM + cells from two mice measured under light isoflurane anesthesia showing active SOM + cells. C , Averaged visual-evoked responses of five SOM + cells shown in A (420 ms stimulus marked with a shaded gray box, averaged over eight directions, eight trials each; mean ± SEM). D , Polar plots of the normalized tuning curves for five SOM + cells shown in A and B (each point represents the mean response over eight trials normalized by the maximum response).

    Article Snippet: Three weeks or more after virus injection, the mice were placed on a warming plate (37°C) and anesthetized with isoflurane (initially 2% and reduced to 1–1.5% during surgery) administered via nose cone (Kopf Instruments).

    Techniques: Mouse Assay, Expressing, Mass Spectrometry

    Schematic illustrating mechanisms of action of isoflurane, 3-mercaptopropionic acid (3-MPA), and nipecotic acid (NPA) within the pontine reticular formation. A. During wakefulness, pontine reticular formation GABA (red dots) is released from vesicles

    Journal: Anesthesiology

    Article Title: GABAergic Neurotransmission in the Pontine Reticular Formation Modulates Hypnosis, Immobility, and Breathing during Isoflurane Anesthesia

    doi: 10.1097/ALN.0b013e31818e3b1b

    Figure Lengend Snippet: Schematic illustrating mechanisms of action of isoflurane, 3-mercaptopropionic acid (3-MPA), and nipecotic acid (NPA) within the pontine reticular formation. A. During wakefulness, pontine reticular formation GABA (red dots) is released from vesicles

    Article Snippet: Briefly, cats were anesthetized by mask induction with isoflurane and placed in a stereotaxic frame (David Kopf Instruments, Tujunga, CA) equipped with a Kopf 880 semi-chronic head holder.

    Techniques:

    GABA levels, electroencephalographic (EEG) power, and neck electromyographic (EMG) power during wakefulness and anesthesia. GABA levels were decreased by isoflurane ( A and B ): A. Isoflurane significantly ( P

    Journal: Anesthesiology

    Article Title: GABAergic Neurotransmission in the Pontine Reticular Formation Modulates Hypnosis, Immobility, and Breathing during Isoflurane Anesthesia

    doi: 10.1097/ALN.0b013e31818e3b1b

    Figure Lengend Snippet: GABA levels, electroencephalographic (EEG) power, and neck electromyographic (EMG) power during wakefulness and anesthesia. GABA levels were decreased by isoflurane ( A and B ): A. Isoflurane significantly ( P

    Article Snippet: Briefly, cats were anesthetized by mask induction with isoflurane and placed in a stereotaxic frame (David Kopf Instruments, Tujunga, CA) equipped with a Kopf 880 semi-chronic head holder.

    Techniques:

    Pontine reticular formation microinjection of drugs that alter GABA levels significantly changed isoflurane induction time and breathing rate. A. The GABA synthesis inhibitor 3-mercaptopropionic acid (3-MPA) significantly ( P

    Journal: Anesthesiology

    Article Title: GABAergic Neurotransmission in the Pontine Reticular Formation Modulates Hypnosis, Immobility, and Breathing during Isoflurane Anesthesia

    doi: 10.1097/ALN.0b013e31818e3b1b

    Figure Lengend Snippet: Pontine reticular formation microinjection of drugs that alter GABA levels significantly changed isoflurane induction time and breathing rate. A. The GABA synthesis inhibitor 3-mercaptopropionic acid (3-MPA) significantly ( P

    Article Snippet: Briefly, cats were anesthetized by mask induction with isoflurane and placed in a stereotaxic frame (David Kopf Instruments, Tujunga, CA) equipped with a Kopf 880 semi-chronic head holder.

    Techniques: