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Developmental Studies Hybridoma Bank islet 1 isl1
Islet 1 Isl1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 85 stars, based on 1 article reviews
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islet 1 isl1 - by Bioz Stars, 2020-09
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Article Title: Insm1 (IA-1) is an essential component of the regulatory network that specifies monoaminergic neuronal phenotypes in the vertebrate hindbrain
Article Snippet: Primary antibodies raised against serotonin (Sigma), Islet 1 (Isl1) (Developmental Studies Hybridoma Bank), Phox2a and Phox2b (gifts from J-F. Brunet and Christo Goridis), Tbx20 (gift from J. Ericson), Foxa2 (Developmental Studies Hybridoma Bank), Nkx2.2 , βIII-tubulin (Covance), BrdU (Developmental Studies Hybridoma Bank), Ascl1 , Tph2 (Sigma), TH (Chemicon), Dbh (Abcam), Tlx3 ( ) and activated caspase 3 (Millipore) were used.

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    Developmental Studies Hybridoma Bank isl1
    The zebrafish sox2 morphant model. ( A ) Lateral view of control embryo at 52 hpf. ( B ) sox2 morphants have small eyes and short body axis in relation to control siblings. ( C ) Microinjections with SOX2 mRNA have no phenotypic effect, while ( D ) mRNA injections into sox2 morphants rescue the phenotypes. ( E ) Western blot showing the reduction in the levels of Sox2 in embryos injected with sox2 -MO1 (second column) and sox2 -MO2 (third column), when compared to controls (first column). β-tubulin was used as loading control. ( F ) Whole mount immunofluorescence for Sox2 (red) and <t>Isl1</t> (blue) shows that Sox2 is expressed in the epithalamus, but not in the differentiated pineal cells at 28 hpf. ( G ) Sox2 is undetectable by immunofluorescence in sox2 morphants, at 28 hpf. ( H–I ) Morpholino microinjections do not grately affect apoptosis, as judged by TUNEL assay at 28 hpf. ( A–D ) scale bars = 250 µm, ( F–G ) scale bars = 25 µm. See also Figure S1 .
    Isl1, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 92/100, based on 49 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/isl1/product/Developmental Studies Hybridoma Bank
    Average 92 stars, based on 49 article reviews
    Price from $9.99 to $1999.99
    isl1 - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    The zebrafish sox2 morphant model. ( A ) Lateral view of control embryo at 52 hpf. ( B ) sox2 morphants have small eyes and short body axis in relation to control siblings. ( C ) Microinjections with SOX2 mRNA have no phenotypic effect, while ( D ) mRNA injections into sox2 morphants rescue the phenotypes. ( E ) Western blot showing the reduction in the levels of Sox2 in embryos injected with sox2 -MO1 (second column) and sox2 -MO2 (third column), when compared to controls (first column). β-tubulin was used as loading control. ( F ) Whole mount immunofluorescence for Sox2 (red) and Isl1 (blue) shows that Sox2 is expressed in the epithalamus, but not in the differentiated pineal cells at 28 hpf. ( G ) Sox2 is undetectable by immunofluorescence in sox2 morphants, at 28 hpf. ( H–I ) Morpholino microinjections do not grately affect apoptosis, as judged by TUNEL assay at 28 hpf. ( A–D ) scale bars = 250 µm, ( F–G ) scale bars = 25 µm. See also Figure S1 .

    Journal: PLoS ONE

    Article Title: Pleiotropic Effects of Sox2 during the Development of the Zebrafish Epithalamus

    doi: 10.1371/journal.pone.0087546

    Figure Lengend Snippet: The zebrafish sox2 morphant model. ( A ) Lateral view of control embryo at 52 hpf. ( B ) sox2 morphants have small eyes and short body axis in relation to control siblings. ( C ) Microinjections with SOX2 mRNA have no phenotypic effect, while ( D ) mRNA injections into sox2 morphants rescue the phenotypes. ( E ) Western blot showing the reduction in the levels of Sox2 in embryos injected with sox2 -MO1 (second column) and sox2 -MO2 (third column), when compared to controls (first column). β-tubulin was used as loading control. ( F ) Whole mount immunofluorescence for Sox2 (red) and Isl1 (blue) shows that Sox2 is expressed in the epithalamus, but not in the differentiated pineal cells at 28 hpf. ( G ) Sox2 is undetectable by immunofluorescence in sox2 morphants, at 28 hpf. ( H–I ) Morpholino microinjections do not grately affect apoptosis, as judged by TUNEL assay at 28 hpf. ( A–D ) scale bars = 250 µm, ( F–G ) scale bars = 25 µm. See also Figure S1 .

    Article Snippet: Series of optical sections from dorsal (first column) to ventral (5th column) obtained using confocal microscope, scale bars = 25 µm, yellow arrows show cells in which sox2 is downregulated and isl1 is upregulated, insets show a three times magnified view of the image.

    Techniques: Western Blot, Injection, Immunofluorescence, TUNEL Assay

    Sox2 inhibits the PhR cell fate. ( A–C ) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs. ( B ) Isl1 labels the pineal cells. ( D–F ) Knockdown of sox2 results in increased number of PhRs. ( G ) The average number of PhRs in control (purple bar) and sox2 morphants (orange bar). ( H–J ) Tg(elavl3:GFP) drives GFP expression specifically in the PNs. ( K–M ) The knockdown of sox2 does not affect the number of PNs. ( N ) The average number of PNs in controls (purple bar) and sox2 morphants (orange bar) does not significantly differ. Confocal maximum projections of 28 hpf embryos, scale bars = 25 µm, error bars represent ± standard error, ** = p-value

    Journal: PLoS ONE

    Article Title: Pleiotropic Effects of Sox2 during the Development of the Zebrafish Epithalamus

    doi: 10.1371/journal.pone.0087546

    Figure Lengend Snippet: Sox2 inhibits the PhR cell fate. ( A–C ) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs. ( B ) Isl1 labels the pineal cells. ( D–F ) Knockdown of sox2 results in increased number of PhRs. ( G ) The average number of PhRs in control (purple bar) and sox2 morphants (orange bar). ( H–J ) Tg(elavl3:GFP) drives GFP expression specifically in the PNs. ( K–M ) The knockdown of sox2 does not affect the number of PNs. ( N ) The average number of PNs in controls (purple bar) and sox2 morphants (orange bar) does not significantly differ. Confocal maximum projections of 28 hpf embryos, scale bars = 25 µm, error bars represent ± standard error, ** = p-value

    Article Snippet: Series of optical sections from dorsal (first column) to ventral (5th column) obtained using confocal microscope, scale bars = 25 µm, yellow arrows show cells in which sox2 is downregulated and isl1 is upregulated, insets show a three times magnified view of the image.

    Techniques: Expressing

    Sox2 expression within the pineal anlage is downregulated with differentiation. ( A–D ) Sox2 expression overlaps with expression of flh , a marker for pineal precursors, at 8 ss. ( E–H ) As pineal cells start differentiating, Isl1 is upregulated whereas Sox2 is downregulated. Yellow arrows show cells that have both Isl1 and Sox2 expression at low levels. ( I–L ) Sox2 is absent from the fully differentiated pineal cells. Scale bars = 25 µm, optical sections from confocal microscopy, insets show a three times magnified view of the image, ( E,I,M ) GFP expression of Tg(flh:GFP) ( F,J,N ) immunofluorescence for Isl1, ( G,K,O ) immunofluorescence for Sox2, ( H,L,P ) merged images with Tg(GFP:flh) in green, Isl1 in blue and Sox2 in red, developmental stages are shown at the beginning of each row.

    Journal: PLoS ONE

    Article Title: Pleiotropic Effects of Sox2 during the Development of the Zebrafish Epithalamus

    doi: 10.1371/journal.pone.0087546

    Figure Lengend Snippet: Sox2 expression within the pineal anlage is downregulated with differentiation. ( A–D ) Sox2 expression overlaps with expression of flh , a marker for pineal precursors, at 8 ss. ( E–H ) As pineal cells start differentiating, Isl1 is upregulated whereas Sox2 is downregulated. Yellow arrows show cells that have both Isl1 and Sox2 expression at low levels. ( I–L ) Sox2 is absent from the fully differentiated pineal cells. Scale bars = 25 µm, optical sections from confocal microscopy, insets show a three times magnified view of the image, ( E,I,M ) GFP expression of Tg(flh:GFP) ( F,J,N ) immunofluorescence for Isl1, ( G,K,O ) immunofluorescence for Sox2, ( H,L,P ) merged images with Tg(GFP:flh) in green, Isl1 in blue and Sox2 in red, developmental stages are shown at the beginning of each row.

    Article Snippet: Series of optical sections from dorsal (first column) to ventral (5th column) obtained using confocal microscope, scale bars = 25 µm, yellow arrows show cells in which sox2 is downregulated and isl1 is upregulated, insets show a three times magnified view of the image.

    Techniques: Expressing, Marker, Confocal Microscopy, Immunofluorescence

    Sox2 and Notch have synergistic effect on neurogenesis and complementary effects on cell-fate determination. ( A–D ) The number of pineal neurons (Isl1-positive cells) is increased in sox2 morphants ( B ) and in DAPT-treated embryos ( C ), when compared to DMSO-treated controls ( A ). A synergistic effect is observed when both sox2 and Notch are knocked down ( D ). ( E–H ) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs in DMSO-treated controls ( E ). The number of PhRs is increased in sox2 morphants ( F ), but remains unaffected in DAPT-treated embryos ( G ). The knockdown of both sox2 and Notch results in an increased number of GFP-positive cells ( H ), comparable to the one observed in sox2 morphants. ( I–L ) Tg(elavl3:GFP) expresses GFP in PNs. There is no difference in the number of GFP-positive in sox2 morphants ( I ) when compared to DMSO-treated controls ( I ). The knockdown of Notch alone ( K ) or simultaneously with sox2 ( L ) results in similar increase in GFP expression. ( M–O ) Average number of Isl1-positive cells ( M ), PhRs ( N ) and PNs ( O ) in untreated controls, DMSO-treated controls, untreated sox2 morphants, DMSO-treated sox2 morphants, DAPT-treated embryos and DAPT-treated sox2 morphants. Confocal maximum projections of 28 hpf embryos, scale bars = 25 µm, error bars represent ± standard error, * = p-value

    Journal: PLoS ONE

    Article Title: Pleiotropic Effects of Sox2 during the Development of the Zebrafish Epithalamus

    doi: 10.1371/journal.pone.0087546

    Figure Lengend Snippet: Sox2 and Notch have synergistic effect on neurogenesis and complementary effects on cell-fate determination. ( A–D ) The number of pineal neurons (Isl1-positive cells) is increased in sox2 morphants ( B ) and in DAPT-treated embryos ( C ), when compared to DMSO-treated controls ( A ). A synergistic effect is observed when both sox2 and Notch are knocked down ( D ). ( E–H ) Tg(aanat2:GFP) drives GFP expression in the pineal PhRs in DMSO-treated controls ( E ). The number of PhRs is increased in sox2 morphants ( F ), but remains unaffected in DAPT-treated embryos ( G ). The knockdown of both sox2 and Notch results in an increased number of GFP-positive cells ( H ), comparable to the one observed in sox2 morphants. ( I–L ) Tg(elavl3:GFP) expresses GFP in PNs. There is no difference in the number of GFP-positive in sox2 morphants ( I ) when compared to DMSO-treated controls ( I ). The knockdown of Notch alone ( K ) or simultaneously with sox2 ( L ) results in similar increase in GFP expression. ( M–O ) Average number of Isl1-positive cells ( M ), PhRs ( N ) and PNs ( O ) in untreated controls, DMSO-treated controls, untreated sox2 morphants, DMSO-treated sox2 morphants, DAPT-treated embryos and DAPT-treated sox2 morphants. Confocal maximum projections of 28 hpf embryos, scale bars = 25 µm, error bars represent ± standard error, * = p-value

    Article Snippet: Series of optical sections from dorsal (first column) to ventral (5th column) obtained using confocal microscope, scale bars = 25 µm, yellow arrows show cells in which sox2 is downregulated and isl1 is upregulated, insets show a three times magnified view of the image.

    Techniques: Expressing

    Knockdown of sox2 results in upregulation of neurogenesis. ( A–B ) Neurogenesis is increased in sox2 morphants, as judged by Isl1 expression. ( C ) Average number of Isl1-positive cells in control (purple bar) and sox2 morphants (orange bar), at 24 and 28 hpf. ( D–E ) At 3–4 ss, two flh -domains are observed on either side of the neural plate. ( F–G ) By 7–8 ss, the two domains fuse to form the presumptive pineal gland. ( H–K ) In sox2 morphants, flh is expressed in broader domains in relation to control siblings. Scale bars = 25 µm, ( A–B ) confocal maximum projections, ( C ) error bars represent ± standard error, ** = p-value

    Journal: PLoS ONE

    Article Title: Pleiotropic Effects of Sox2 during the Development of the Zebrafish Epithalamus

    doi: 10.1371/journal.pone.0087546

    Figure Lengend Snippet: Knockdown of sox2 results in upregulation of neurogenesis. ( A–B ) Neurogenesis is increased in sox2 morphants, as judged by Isl1 expression. ( C ) Average number of Isl1-positive cells in control (purple bar) and sox2 morphants (orange bar), at 24 and 28 hpf. ( D–E ) At 3–4 ss, two flh -domains are observed on either side of the neural plate. ( F–G ) By 7–8 ss, the two domains fuse to form the presumptive pineal gland. ( H–K ) In sox2 morphants, flh is expressed in broader domains in relation to control siblings. Scale bars = 25 µm, ( A–B ) confocal maximum projections, ( C ) error bars represent ± standard error, ** = p-value

    Article Snippet: Series of optical sections from dorsal (first column) to ventral (5th column) obtained using confocal microscope, scale bars = 25 µm, yellow arrows show cells in which sox2 is downregulated and isl1 is upregulated, insets show a three times magnified view of the image.

    Techniques: Expressing

    Shh Agonist Acts as a Motor Neuronal Differentiation Factor Equivalent to Shh Protein. (A) ATRA (1 µM) and SAG (10 to 1,000 nM) treatments as well as 1 µM ATRA and 500 ng/µl Shh treatment greatly increased both HB9+ and Is11+ cells. (B) Isl1/βIII-tubulin double-positive cells were observed in ATRA/SAG culture. White bar indicates 20 µm. (C–G) Quantitation of the gene expression levels in ATRA/SAG-treated culture. Two sMN-specific markers, HB9 and Isl1, were upregulated by ATRA/SAG treatment (C, D). A spinal cord marker, HoxB4, was also upregulated (E), while a forebrain marker, BF1, was downregulated by addition of ATRA/SAG (10 and 100 nM) (F). The change in expression of the neural cell marker βIII-tubulin was not statistically significant (G). *p

    Journal: PLoS ONE

    Article Title: Highly Efficient Differentiation and Enrichment of Spinal Motor Neurons Derived from Human and Monkey Embryonic Stem Cells

    doi: 10.1371/journal.pone.0006722

    Figure Lengend Snippet: Shh Agonist Acts as a Motor Neuronal Differentiation Factor Equivalent to Shh Protein. (A) ATRA (1 µM) and SAG (10 to 1,000 nM) treatments as well as 1 µM ATRA and 500 ng/µl Shh treatment greatly increased both HB9+ and Is11+ cells. (B) Isl1/βIII-tubulin double-positive cells were observed in ATRA/SAG culture. White bar indicates 20 µm. (C–G) Quantitation of the gene expression levels in ATRA/SAG-treated culture. Two sMN-specific markers, HB9 and Isl1, were upregulated by ATRA/SAG treatment (C, D). A spinal cord marker, HoxB4, was also upregulated (E), while a forebrain marker, BF1, was downregulated by addition of ATRA/SAG (10 and 100 nM) (F). The change in expression of the neural cell marker βIII-tubulin was not statistically significant (G). *p

    Article Snippet: The gene expression levels of sMN markers such as HB9, Isl1 and Olig2 were upregulated 1.3-, 8.5- and 5.2-fold, respectively, by ATRA/Shh treatment , and also a caudal brain marker, HoxB4, was drastically upregulated ( ).

    Techniques: Quantitation Assay, Expressing, Marker

    ATRA/Shh Promotes Differentiation to Spinal Cord Motor Neurons. Dissociated NRs, derived from hESCs or monkey ESCs, were cultured in the presence of ATRA and Shh for 7 days. When 1 µM ATRA and 500 ng/ml Shh were supplemented in the differentiation culture (B, D, F and H), larger numbers of βIII-tubulin+/HB9+ (B and F) and βIII-tubulin+/Isl1+ cells (D and H) were observed. Insets in A–H show high magnification. (I and J) The ratio of HB9- or Isl1-positive cells was calculated from the hESC differentiation culture (I) and monkey ESC differentiation culture (J). Scale bar in A indicates 100 µm. Inset scale bar indicates 10 µm. *p

    Journal: PLoS ONE

    Article Title: Highly Efficient Differentiation and Enrichment of Spinal Motor Neurons Derived from Human and Monkey Embryonic Stem Cells

    doi: 10.1371/journal.pone.0006722

    Figure Lengend Snippet: ATRA/Shh Promotes Differentiation to Spinal Cord Motor Neurons. Dissociated NRs, derived from hESCs or monkey ESCs, were cultured in the presence of ATRA and Shh for 7 days. When 1 µM ATRA and 500 ng/ml Shh were supplemented in the differentiation culture (B, D, F and H), larger numbers of βIII-tubulin+/HB9+ (B and F) and βIII-tubulin+/Isl1+ cells (D and H) were observed. Insets in A–H show high magnification. (I and J) The ratio of HB9- or Isl1-positive cells was calculated from the hESC differentiation culture (I) and monkey ESC differentiation culture (J). Scale bar in A indicates 100 µm. Inset scale bar indicates 10 µm. *p

    Article Snippet: The gene expression levels of sMN markers such as HB9, Isl1 and Olig2 were upregulated 1.3-, 8.5- and 5.2-fold, respectively, by ATRA/Shh treatment , and also a caudal brain marker, HoxB4, was drastically upregulated ( ).

    Techniques: Derivative Assay, Cell Culture

    ATRA/Shh Induces the Expression of Spinal Cord-Specific and Motor Neuron-Specific Genes. (A–C) The expression of sMN-specific genes such as HB9, Isl1, and Olig2 was upregulated by the treatment of 1 µM ATRA and 500 ng/ml Shh. (D) A spinal cord-specific gene, HoxB4, was also upregulated in ATRA/SAG-treated cells compared to the control without ATRA/Shh. In contrast, the rostral brain marker BF1 expression was downregulated in ATRA/Shh-treated culture compared to the control culture (E). (F) There was no statistically significant difference in the expression levels of the neuronal marker βIII-tubulin between ATRA/Shh culture and the control. The gene expression levels in the control culture were defined as 1.0.*p

    Journal: PLoS ONE

    Article Title: Highly Efficient Differentiation and Enrichment of Spinal Motor Neurons Derived from Human and Monkey Embryonic Stem Cells

    doi: 10.1371/journal.pone.0006722

    Figure Lengend Snippet: ATRA/Shh Induces the Expression of Spinal Cord-Specific and Motor Neuron-Specific Genes. (A–C) The expression of sMN-specific genes such as HB9, Isl1, and Olig2 was upregulated by the treatment of 1 µM ATRA and 500 ng/ml Shh. (D) A spinal cord-specific gene, HoxB4, was also upregulated in ATRA/SAG-treated cells compared to the control without ATRA/Shh. In contrast, the rostral brain marker BF1 expression was downregulated in ATRA/Shh-treated culture compared to the control culture (E). (F) There was no statistically significant difference in the expression levels of the neuronal marker βIII-tubulin between ATRA/Shh culture and the control. The gene expression levels in the control culture were defined as 1.0.*p

    Article Snippet: The gene expression levels of sMN markers such as HB9, Isl1 and Olig2 were upregulated 1.3-, 8.5- and 5.2-fold, respectively, by ATRA/Shh treatment , and also a caudal brain marker, HoxB4, was drastically upregulated ( ).

    Techniques: Expressing, Marker