irdye 800cw goat anti mouse igg h l  (LI-COR)

 
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    Name:
    IRDye 800CW Goat anti Mouse IgG H L
    Description:
    Highly cross adsorbed secondary antibody Suitable for a variety of applications including Western blotting In Cell Western Assays In Gel Westerns and many others
    Catalog Number:
    925-32210
    Price:
    None
    Category:
    IRDye secondary antibody
    Applications:
    Western blot, In-Cell Western assay, In-Gel Westerns, in vivo imaging
    Conjugate:
    IRDye 800CW
    Size:
    1
    Quantity:
    1
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    Structured Review

    LI-COR irdye 800cw goat anti mouse igg h l
    IRDye 800CW Goat anti Mouse IgG H L
    Highly cross adsorbed secondary antibody Suitable for a variety of applications including Western blotting In Cell Western Assays In Gel Westerns and many others
    https://www.bioz.com/result/irdye 800cw goat anti mouse igg h l/product/LI-COR
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    irdye 800cw goat anti mouse igg h l - by Bioz Stars, 2021-04
    99/100 stars

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    Related Articles

    Fluorescence:

    Article Title: Bovine Adenovirus-3 pVIII Suppresses Cap-Dependent mRNA Translation Possibly by Interfering with the Recruitment of DDX3 and Translation Initiation Factors to the mRNA Cap
    Article Snippet: Anti-DBP recognizes a protein of 48 kDa in BAdV-3 infected cells ( ). .. Anti-DDX3, anti-eIF4G, anti-eIF4E, anti-eIF3, anti-PABP and fluorescence conjugated goat anti-mouse IgG-FITC (Santa Cruz Biotechnology, Inc., USA), Cy-3 conjugated goat anti-rabbit antibody (Jackson Immuno Research), Alexa Flour 680 goat anti-rabbit IgG antibody (Molecular Probes) or IRDye 800 conjugated goat anti-mouse IgG (Li-COR biosciences) and anti-HA and anti-β-actin MAb (Sigma Aldrich) were purchased. .. Plasmid Construction Plasmids were constructed (Supplementary File) using standard procedures.

    other:

    Article Title: Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus
    Article Snippet: IRDye® 800CW Goat anti-Mouse and anti-Rabbit antibodies, and IRDye 680RD Goat anti-Mouse IgG were purchased from LI-COR.

    Article Title: Novel monoclonal antibodies to the SERINC5 HIV-1 restriction factor detect endogenous and virion-associated SERINC5
    Article Snippet: Background wells contained only IRDye 800CW-secondary goat anti-mouse antibody.

    Blocking Assay:

    Article Title: The Effect of Human Factor H on Immunogenicity of Meningococcal Native Outer Membrane Vesicle Vaccines with Over-Expressed Factor H Binding Protein
    Article Snippet: For the Western blot, the proteins were transferred to a polyvinylidene fluoride membrane (Immobilon-FL; Millipore) using an XCell II Blot Module (Invitrogen). .. The membrane was blocked using PBS (Roche) containing 1% (w/v) blocking grade nonfat dry milk (Bio-Rad), and then washed with PBS containing 0.1% Tween-20 (Sigma). fHbp was detected using anti-fHbp mAb JAR 5 (0.1 µg/ml) and goat anti-mouse IgG conjugated to IRDye 800CW (Li-Cor Biosciences). .. The IRDye was detected at 800-nm wavelength on an infrared scanner (Odyssey; Li-Cor Biosciences).

    Article Title: A Novel Method to Titrate Herpes Simplex Virus-1 (HSV-1) Using Laser-Based Scanning of Near-Infrared Fluorophores Conjugated Antibodies
    Article Snippet: Following the incubation with Odyssey Blocking Buffer for 1 h at rt, cells were incubated with anti-glycoprotein B (gB) or anti-ICP4 antibodies (sc-56987 and sc-S9808N, Santa Cruz, respectively, 1:1000 dilution in Odyssey Blocking buffer) for 1 h and then washed three times with PBS containing 0.1% Tween-20. .. Afterward, labeled secondary antibody IRDye 800 CW Goat Anti Mouse (926-32210 LI-COR Biosciences, 1:1000 dilution in Odyssey Blocking buffer) and CellTag 700 Stain (926-41090, LI-COR Biosciences, 1:500) were added to each well, and after 1 h, cells were washed four times with PBS containing 0.1% Tween-20. .. Finally, the plate was scanned on the Odyssey Infrared Imager, and the integrated intensity value of each well read by LI-COR Image Studio Software developed for Odyssey analysis.

    Labeling:

    Article Title: A Novel Method to Titrate Herpes Simplex Virus-1 (HSV-1) Using Laser-Based Scanning of Near-Infrared Fluorophores Conjugated Antibodies
    Article Snippet: Following the incubation with Odyssey Blocking Buffer for 1 h at rt, cells were incubated with anti-glycoprotein B (gB) or anti-ICP4 antibodies (sc-56987 and sc-S9808N, Santa Cruz, respectively, 1:1000 dilution in Odyssey Blocking buffer) for 1 h and then washed three times with PBS containing 0.1% Tween-20. .. Afterward, labeled secondary antibody IRDye 800 CW Goat Anti Mouse (926-32210 LI-COR Biosciences, 1:1000 dilution in Odyssey Blocking buffer) and CellTag 700 Stain (926-41090, LI-COR Biosciences, 1:500) were added to each well, and after 1 h, cells were washed four times with PBS containing 0.1% Tween-20. .. Finally, the plate was scanned on the Odyssey Infrared Imager, and the integrated intensity value of each well read by LI-COR Image Studio Software developed for Odyssey analysis.

    Staining:

    Article Title: A Novel Method to Titrate Herpes Simplex Virus-1 (HSV-1) Using Laser-Based Scanning of Near-Infrared Fluorophores Conjugated Antibodies
    Article Snippet: Following the incubation with Odyssey Blocking Buffer for 1 h at rt, cells were incubated with anti-glycoprotein B (gB) or anti-ICP4 antibodies (sc-56987 and sc-S9808N, Santa Cruz, respectively, 1:1000 dilution in Odyssey Blocking buffer) for 1 h and then washed three times with PBS containing 0.1% Tween-20. .. Afterward, labeled secondary antibody IRDye 800 CW Goat Anti Mouse (926-32210 LI-COR Biosciences, 1:1000 dilution in Odyssey Blocking buffer) and CellTag 700 Stain (926-41090, LI-COR Biosciences, 1:500) were added to each well, and after 1 h, cells were washed four times with PBS containing 0.1% Tween-20. .. Finally, the plate was scanned on the Odyssey Infrared Imager, and the integrated intensity value of each well read by LI-COR Image Studio Software developed for Odyssey analysis.

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    LI-COR irdye 800cw goat anti mouse
    Detection of YFV structural and non-structural proteins by Western blot assay . Vero cells were mock-infected or infected with YFV 17D at a MOI of 1 for 48 h ( A ). Huh-7.5 cells were mock-infected or infected with YFV Asibi at a MOI of 2.5 for 40 h ( B ). Proteins in the cell lysates were resolved on 4-12% NuPAGE Novex gels and transferred to nitrocellulose membranes. Shown in green are the bands detected after incubation with the indicated rabbit polyclonal primary antibodies against YFV proteins and the <t>IRDye</t> 800CW Goat anti-Rabbit IgG secondary antibody. β-actin, shown in red, is an internal control, and was detected by a mouse monoclonal primary antibody and the IRDye 680RD Goat anti-Mouse IgG secondary antibody. A pre-stained protein molecular weight marker, shown on the left, is used to estimate the size of proteins resolved by gel electrophoresis. The band expected for each of the YFV proteins is indicated on the right.
    Irdye 800cw Goat Anti Mouse, supplied by LI-COR, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/irdye 800cw goat anti mouse/product/LI-COR
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    irdye 800cw goat anti mouse - by Bioz Stars, 2021-04
    97/100 stars
      Buy from Supplier

    Image Search Results


    Detection of YFV structural and non-structural proteins by Western blot assay . Vero cells were mock-infected or infected with YFV 17D at a MOI of 1 for 48 h ( A ). Huh-7.5 cells were mock-infected or infected with YFV Asibi at a MOI of 2.5 for 40 h ( B ). Proteins in the cell lysates were resolved on 4-12% NuPAGE Novex gels and transferred to nitrocellulose membranes. Shown in green are the bands detected after incubation with the indicated rabbit polyclonal primary antibodies against YFV proteins and the IRDye 800CW Goat anti-Rabbit IgG secondary antibody. β-actin, shown in red, is an internal control, and was detected by a mouse monoclonal primary antibody and the IRDye 680RD Goat anti-Mouse IgG secondary antibody. A pre-stained protein molecular weight marker, shown on the left, is used to estimate the size of proteins resolved by gel electrophoresis. The band expected for each of the YFV proteins is indicated on the right.

    Journal: Antiviral Research

    Article Title: Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus

    doi: 10.1016/j.antiviral.2020.104907

    Figure Lengend Snippet: Detection of YFV structural and non-structural proteins by Western blot assay . Vero cells were mock-infected or infected with YFV 17D at a MOI of 1 for 48 h ( A ). Huh-7.5 cells were mock-infected or infected with YFV Asibi at a MOI of 2.5 for 40 h ( B ). Proteins in the cell lysates were resolved on 4-12% NuPAGE Novex gels and transferred to nitrocellulose membranes. Shown in green are the bands detected after incubation with the indicated rabbit polyclonal primary antibodies against YFV proteins and the IRDye 800CW Goat anti-Rabbit IgG secondary antibody. β-actin, shown in red, is an internal control, and was detected by a mouse monoclonal primary antibody and the IRDye 680RD Goat anti-Mouse IgG secondary antibody. A pre-stained protein molecular weight marker, shown on the left, is used to estimate the size of proteins resolved by gel electrophoresis. The band expected for each of the YFV proteins is indicated on the right.

    Article Snippet: IRDye® 800CW Goat anti-Mouse and anti-Rabbit antibodies, and IRDye 680RD Goat anti-Mouse IgG were purchased from LI-COR.

    Techniques: Western Blot, Infection, Incubation, Staining, Molecular Weight, Marker, Nucleic Acid Electrophoresis

    Establishment of two YFV NS4B antibody-based antiviral assays . Huh-7 cells were seeded on 96- well (A) or 384-well plates (B) and infected with YFV 17D at a MOI of 1, with either mock or indicated treatment, for 48 h. (A) In-cell western assay. YFV proteins were detected with indicated antibody against NS3, NS4B or pan-flavivirus monoclonal antibody 4G2 as primary antibody, with IRDye® 800CW goat anti-Rabbit or anti-Mouse as secondary antibodies. Cells were stained with DRAQ5 and Sapphire700. YFV protein expression (green) and cell viability (red) were visualized in LI-COR Odyssey. (B and C) High-content imaging assay. Representative images of NS4B and DAPI staining are shown as a function of doses of BDAA treatment (B). Quantitation of percentage of cells with positive NS4B signal and total fluorescence intensity were calculated based on multiple images taken from each well (n=6) and expressed as average ± standard deviation. * indicates P

    Journal: Antiviral Research

    Article Title: Development of antibody-based assays for high throughput discovery and mechanistic study of antiviral agents against yellow fever virus

    doi: 10.1016/j.antiviral.2020.104907

    Figure Lengend Snippet: Establishment of two YFV NS4B antibody-based antiviral assays . Huh-7 cells were seeded on 96- well (A) or 384-well plates (B) and infected with YFV 17D at a MOI of 1, with either mock or indicated treatment, for 48 h. (A) In-cell western assay. YFV proteins were detected with indicated antibody against NS3, NS4B or pan-flavivirus monoclonal antibody 4G2 as primary antibody, with IRDye® 800CW goat anti-Rabbit or anti-Mouse as secondary antibodies. Cells were stained with DRAQ5 and Sapphire700. YFV protein expression (green) and cell viability (red) were visualized in LI-COR Odyssey. (B and C) High-content imaging assay. Representative images of NS4B and DAPI staining are shown as a function of doses of BDAA treatment (B). Quantitation of percentage of cells with positive NS4B signal and total fluorescence intensity were calculated based on multiple images taken from each well (n=6) and expressed as average ± standard deviation. * indicates P

    Article Snippet: IRDye® 800CW Goat anti-Mouse and anti-Rabbit antibodies, and IRDye 680RD Goat anti-Mouse IgG were purchased from LI-COR.

    Techniques: Infection, In-Cell ELISA, Staining, Expressing, Imaging, Quantitation Assay, Fluorescence, Standard Deviation

    Detection of SERINC5 in HEK293 cells transduced to overexpress SERINC5 isoform 1 (HEK293_S5.1). (a) HEK293_S5.1 cell extract was applied to two preparative wells of a 12% polyacrylamide gel, with a center lane containing labeled molecular weight standards. The MPX blotting system was applied to the blot to screen for mAb reactivity with (lanes 6–10) or without (lanes 1–5) competition with the matched immunizing peptides at 10 μg/mL. The mAbs were added at 0.5 μg/mL to lanes in the MPX as follows: 14C1-1 (1 and 6); 18B6-1 (2 and 7); 23E4-1 (3 and 8); 28E8-2 (4 and 9); anti-DDK plus a mix of all three peptides (5 and 10). The mAbs were detected using goat anti-mouse IgG-IRDye 800CW (green bands). The rabbit anti-clathrin heavy chain (HC) was added to all lanes and detected with goat anti-rabbit IgG-IRDye 680RD (red bands), as a protein loading control. (b). MFI of flow cytometry surface staining of HEK293_S5.1 with AF647 conjugated mAbs, as indicated; intracellular detection was performed using 28E8-2 (ICL4). Histograms indicate staining of the cells with (blue peaks) or without (red peaks) mAb pre-incubation with the respective SERINC5 peptides.

    Journal: mAbs

    Article Title: Novel monoclonal antibodies to the SERINC5 HIV-1 restriction factor detect endogenous and virion-associated SERINC5

    doi: 10.1080/19420862.2020.1802187

    Figure Lengend Snippet: Detection of SERINC5 in HEK293 cells transduced to overexpress SERINC5 isoform 1 (HEK293_S5.1). (a) HEK293_S5.1 cell extract was applied to two preparative wells of a 12% polyacrylamide gel, with a center lane containing labeled molecular weight standards. The MPX blotting system was applied to the blot to screen for mAb reactivity with (lanes 6–10) or without (lanes 1–5) competition with the matched immunizing peptides at 10 μg/mL. The mAbs were added at 0.5 μg/mL to lanes in the MPX as follows: 14C1-1 (1 and 6); 18B6-1 (2 and 7); 23E4-1 (3 and 8); 28E8-2 (4 and 9); anti-DDK plus a mix of all three peptides (5 and 10). The mAbs were detected using goat anti-mouse IgG-IRDye 800CW (green bands). The rabbit anti-clathrin heavy chain (HC) was added to all lanes and detected with goat anti-rabbit IgG-IRDye 680RD (red bands), as a protein loading control. (b). MFI of flow cytometry surface staining of HEK293_S5.1 with AF647 conjugated mAbs, as indicated; intracellular detection was performed using 28E8-2 (ICL4). Histograms indicate staining of the cells with (blue peaks) or without (red peaks) mAb pre-incubation with the respective SERINC5 peptides.

    Article Snippet: Background wells contained only IRDye 800CW-secondary goat anti-mouse antibody.

    Techniques: Labeling, Molecular Weight, Flow Cytometry, Staining, Incubation

    Western blotting with infrared dye–labeled anti-IgG nanobodies. (a) A twofold dilution series of Xenopus egg extract was analyzed by SDS-PAGE and Western blotting. The indicated rabbit polyclonal antibodies were used to detect Nups. These primary antibodies were then decorated via either IRDye 800–labeled goat anti–rabbit polyclonal IgG (1:5,000; LI-COR Biosciences) or anti–rabbit IgG nanobody TP897 (10 nM). Blots were analyzed with an Odyssey Infrared Imaging System (LI-COR Biosciences). (b) Left: A twofold dilution series of HeLa cell lysate was analyzed by SDS-PAGE and Western blotting. The indicated mouse IgG1 mAbs were decorated via either IRDye 800–labeled goat anti–mouse polyclonal IgG (1:1,340, 5 nM; LI-COR Biosciences) or anti–mouse IgG1 Fc nanobody TP1107 (5 nM). Right: A twofold dilution series of Xenopus egg extract was blotted and probed with anti-Nup62 mouse IgG1 mAb A225. It was then detected via IRDye 800–labeled goat anti-mouse polyclonal IgG (5 nM), anti–mouse IgG1 Fc nanobody TP1107 (5 nM), anti–mouse IgG1 Fab nanobody TP886 (5 nM), anti–mouse κ chain nanobody TP1170 (2.5 nM), or a combination of TP1107 and TP886 or TP1107 and TP1170. Blue pixels indicate signal saturation. (c) A dilution series of filamentous bacteriophages was blotted and probed with an anti–minor coat protein pIII mouse IgG2a mAb. It was then decorated via either IRDye 800–labeled goat anti-mouse polyclonal IgG (2.5 nM) or anti–mouse κ chain nanobody TP1170 (2.5 nM). (d) Dual-color Western blotting. A twofold dilution series of Xenopus egg extract was blotted and probed with anti-Nup62 mouse IgG1 mAb A225 and rabbit anti-Nup54 polyclonal antibody. These primary antibodies were then detected via IRDye 800–labeled goat anti–rabbit polyclonal IgG and IRDye 680–labeled goat anti–mouse polyclonal IgG. Alternatively, they were detected with TP1107 coupled to IRDye 680 and TP897 coupled to IRDye 800.

    Journal: The Journal of Cell Biology

    Article Title: A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies

    doi: 10.1083/jcb.201709115

    Figure Lengend Snippet: Western blotting with infrared dye–labeled anti-IgG nanobodies. (a) A twofold dilution series of Xenopus egg extract was analyzed by SDS-PAGE and Western blotting. The indicated rabbit polyclonal antibodies were used to detect Nups. These primary antibodies were then decorated via either IRDye 800–labeled goat anti–rabbit polyclonal IgG (1:5,000; LI-COR Biosciences) or anti–rabbit IgG nanobody TP897 (10 nM). Blots were analyzed with an Odyssey Infrared Imaging System (LI-COR Biosciences). (b) Left: A twofold dilution series of HeLa cell lysate was analyzed by SDS-PAGE and Western blotting. The indicated mouse IgG1 mAbs were decorated via either IRDye 800–labeled goat anti–mouse polyclonal IgG (1:1,340, 5 nM; LI-COR Biosciences) or anti–mouse IgG1 Fc nanobody TP1107 (5 nM). Right: A twofold dilution series of Xenopus egg extract was blotted and probed with anti-Nup62 mouse IgG1 mAb A225. It was then detected via IRDye 800–labeled goat anti-mouse polyclonal IgG (5 nM), anti–mouse IgG1 Fc nanobody TP1107 (5 nM), anti–mouse IgG1 Fab nanobody TP886 (5 nM), anti–mouse κ chain nanobody TP1170 (2.5 nM), or a combination of TP1107 and TP886 or TP1107 and TP1170. Blue pixels indicate signal saturation. (c) A dilution series of filamentous bacteriophages was blotted and probed with an anti–minor coat protein pIII mouse IgG2a mAb. It was then decorated via either IRDye 800–labeled goat anti-mouse polyclonal IgG (2.5 nM) or anti–mouse κ chain nanobody TP1170 (2.5 nM). (d) Dual-color Western blotting. A twofold dilution series of Xenopus egg extract was blotted and probed with anti-Nup62 mouse IgG1 mAb A225 and rabbit anti-Nup54 polyclonal antibody. These primary antibodies were then detected via IRDye 800–labeled goat anti–rabbit polyclonal IgG and IRDye 680–labeled goat anti–mouse polyclonal IgG. Alternatively, they were detected with TP1107 coupled to IRDye 680 and TP897 coupled to IRDye 800.

    Article Snippet: Polyclonal goat anti–mouse IgG coupled to IRDye 800CW (925-32210; LI-COR Biosciences) was used to detect primary mouse antibodies at a dilution of 1:1,340 (5 nM).

    Techniques: Western Blot, Labeling, SDS Page, Imaging

    Proteins with polyQ of different lengths show different characteristics. A Schematic representation of the constructs used to express polyQ proteins. Each contains a FLAG tag at its N-terminus, followed by huntingtin's exon 1 encoding the first 17 amino acids (Htt), a polyQ tract of different length, and GFP at the C-terminus. Expression of all polyQ proteins is driven by the pGal1 promoter that is induced by galactose and repressed by glucose. B Wild-type cells (W303–1b) expressing the 25Q, 47Q or 103Q were grown logarithmically (0.6–1 A 600 ) for 8 hours under galactose induction. Cell lysates were resolved by SDS-PAGE and polyQ proteins were detected by immunoblotting (IB) with a mouse anti-FLAG antibody followed by IRDye 800CW-conjugated goat anti-mouse IgG (right panel) or a rabbit anti-GFP followed by Dylight 680-labeled goat anti-rabbit IgG (left panel, red). A mouse anti-actin followed by IRDye 800CW-conjugated goat anti-mouse IgG was used as a loading control (left panel, green). Blots were visualized by the Odyssey Infrared Imaging System. C Wild-type cells (W303–1b) expressing the 25Q, 47Q, 103Q or an empty plasmid were grown logarithmically in glucose and 10-fold serial dilutions (starting with 7.5×10 6 cells) were spotted on glucose or galactose plates.

    Journal: PLoS ONE

    Article Title: Aggregation of PolyQ Proteins Is Increased upon Yeast Aging and Affected by Sir2 and Hsf1: Novel Quantitative Biochemical and Microscopic Assays

    doi: 10.1371/journal.pone.0044785

    Figure Lengend Snippet: Proteins with polyQ of different lengths show different characteristics. A Schematic representation of the constructs used to express polyQ proteins. Each contains a FLAG tag at its N-terminus, followed by huntingtin's exon 1 encoding the first 17 amino acids (Htt), a polyQ tract of different length, and GFP at the C-terminus. Expression of all polyQ proteins is driven by the pGal1 promoter that is induced by galactose and repressed by glucose. B Wild-type cells (W303–1b) expressing the 25Q, 47Q or 103Q were grown logarithmically (0.6–1 A 600 ) for 8 hours under galactose induction. Cell lysates were resolved by SDS-PAGE and polyQ proteins were detected by immunoblotting (IB) with a mouse anti-FLAG antibody followed by IRDye 800CW-conjugated goat anti-mouse IgG (right panel) or a rabbit anti-GFP followed by Dylight 680-labeled goat anti-rabbit IgG (left panel, red). A mouse anti-actin followed by IRDye 800CW-conjugated goat anti-mouse IgG was used as a loading control (left panel, green). Blots were visualized by the Odyssey Infrared Imaging System. C Wild-type cells (W303–1b) expressing the 25Q, 47Q, 103Q or an empty plasmid were grown logarithmically in glucose and 10-fold serial dilutions (starting with 7.5×10 6 cells) were spotted on glucose or galactose plates.

    Article Snippet: The secondary antibody used was IRDye 800CW-conjugated goat anti-mouse (LI-COR Biosciences).

    Techniques: Construct, FLAG-tag, Expressing, SDS Page, Labeling, Imaging, Plasmid Preparation