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Abcam anti irak3
Anti Irak3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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anti irak3 - by Bioz Stars, 2024-07
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Abcam irak3
Irak3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irak3/product/Abcam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
irak3 - by Bioz Stars, 2024-07
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Abcam irak3
<t>IRAK3</t> was a downstream target of miR-591. a The predicted binding sites of miR-591 in the 3′UTR of IRAK3 mRNA were identified by using the starBase database and the relative luciferase activity in chondrocytes co-transfected with wt-IRAK3 or mut-IRAK3 and miR-591 mimic or miRNA NC was determined by dual-luciferase reporter assay. b The level of miR-591 was determined in OA chondrocytes transfected with NC, miR-591, anti-NC, or anti-miR-591. c The level of IRAK3 protein expression level was determined in OA chondrocytes transfected with NC, miR-591, anti-NC, or anti-miR-591. d The IRAK3 mRNA expression in OA cartilage and normal cartilage. e The IRAK3 protein expression in OA cartilage and normal cartilage. * P <0.05
Irak3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/irak3/product/Abcam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
irak3 - by Bioz Stars, 2024-07
86/100 stars

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1) Product Images from "Knockdown of Circ_SLC39A8 protects against the progression of osteoarthritis by regulating miR-591/IRAK3 axis"

Article Title: Knockdown of Circ_SLC39A8 protects against the progression of osteoarthritis by regulating miR-591/IRAK3 axis

Journal: Journal of Orthopaedic Surgery and Research

doi: 10.1186/s13018-021-02323-7

IRAK3 was a downstream target of miR-591. a The predicted binding sites of miR-591 in the 3′UTR of IRAK3 mRNA were identified by using the starBase database and the relative luciferase activity in chondrocytes co-transfected with wt-IRAK3 or mut-IRAK3 and miR-591 mimic or miRNA NC was determined by dual-luciferase reporter assay. b The level of miR-591 was determined in OA chondrocytes transfected with NC, miR-591, anti-NC, or anti-miR-591. c The level of IRAK3 protein expression level was determined in OA chondrocytes transfected with NC, miR-591, anti-NC, or anti-miR-591. d The IRAK3 mRNA expression in OA cartilage and normal cartilage. e The IRAK3 protein expression in OA cartilage and normal cartilage. * P <0.05
Figure Legend Snippet: IRAK3 was a downstream target of miR-591. a The predicted binding sites of miR-591 in the 3′UTR of IRAK3 mRNA were identified by using the starBase database and the relative luciferase activity in chondrocytes co-transfected with wt-IRAK3 or mut-IRAK3 and miR-591 mimic or miRNA NC was determined by dual-luciferase reporter assay. b The level of miR-591 was determined in OA chondrocytes transfected with NC, miR-591, anti-NC, or anti-miR-591. c The level of IRAK3 protein expression level was determined in OA chondrocytes transfected with NC, miR-591, anti-NC, or anti-miR-591. d The IRAK3 mRNA expression in OA cartilage and normal cartilage. e The IRAK3 protein expression in OA cartilage and normal cartilage. * P <0.05

Techniques Used: Binding Assay, Luciferase, Activity Assay, Transfection, Reporter Assay, Expressing

Overexpression of IRAK3 partially reversed miR-591 on chondrocyte apoptosis. a Overexpression efficiency of IRAK3 was determined by western blot assay in OA chondrocytes transfected with IRAK3 or vector. b IRAK expression in OA chondrocytes transfected with NC, miR-591, miR-591 + vector, or miR-591 + IRAK3. c Cell apoptosis was determined using flow cytometry analysis. d The protein levels of Bcl-2, Bax, caspase 3, cleaved-caspase 3, caspase 9, and cleaved-caspase 9 were analyzed by western blot assay. e Western blot assay was carried out to examine the protein expression of TNF-α, IL-1β, IL-6, MMP3, and COL2A1. * P <0.05
Figure Legend Snippet: Overexpression of IRAK3 partially reversed miR-591 on chondrocyte apoptosis. a Overexpression efficiency of IRAK3 was determined by western blot assay in OA chondrocytes transfected with IRAK3 or vector. b IRAK expression in OA chondrocytes transfected with NC, miR-591, miR-591 + vector, or miR-591 + IRAK3. c Cell apoptosis was determined using flow cytometry analysis. d The protein levels of Bcl-2, Bax, caspase 3, cleaved-caspase 3, caspase 9, and cleaved-caspase 9 were analyzed by western blot assay. e Western blot assay was carried out to examine the protein expression of TNF-α, IL-1β, IL-6, MMP3, and COL2A1. * P <0.05

Techniques Used: Over Expression, Western Blot, Transfection, Plasmid Preparation, Expressing, Flow Cytometry


Structured Review

Abcam anti irak3 antibody
qRT-PCR Primers
Anti Irak3 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti irak3 antibody/product/Abcam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti irak3 antibody - by Bioz Stars, 2024-07
86/100 stars

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1) Product Images from "Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma"

Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

Journal: Cancer Management and Research

doi: 10.2147/CMAR.S252772

qRT-PCR Primers
Figure Legend Snippet: qRT-PCR Primers

Techniques Used: Sequencing

Methylation-Specific Primers
Figure Legend Snippet: Methylation-Specific Primers

Techniques Used: Methylation, Sequencing

DNA methylation analysis of tumor tissues and normal tissues and analysis of methylation degree for seven CpG sites of IRAK3 . ( A ) The expression of the top 20 candidate genes was analyzed. IRAK3 was hypermethylated significantly in tumor tissues compared with normal tissues. ( B ) The number of IRAK3 –methylated CpG islands in each isosite. ( C – I ) Seven CpG sites for IRAK3 , which are presented in the boxplot, displayed a decreased methylation in the tumor group. The boxplot for cg 26,415,547 ( C ), cg 26,279,550 ( D ), cg 20,162,652 ( E ), cg 18,177,616 ( F ), cg 13,807,985 ( G ), cg 10,389,229 ( H ), and cg 01263292 ( I ). Abbreviation: MG, malignant glioma.
Figure Legend Snippet: DNA methylation analysis of tumor tissues and normal tissues and analysis of methylation degree for seven CpG sites of IRAK3 . ( A ) The expression of the top 20 candidate genes was analyzed. IRAK3 was hypermethylated significantly in tumor tissues compared with normal tissues. ( B ) The number of IRAK3 –methylated CpG islands in each isosite. ( C – I ) Seven CpG sites for IRAK3 , which are presented in the boxplot, displayed a decreased methylation in the tumor group. The boxplot for cg 26,415,547 ( C ), cg 26,279,550 ( D ), cg 20,162,652 ( E ), cg 18,177,616 ( F ), cg 13,807,985 ( G ), cg 10,389,229 ( H ), and cg 01263292 ( I ). Abbreviation: MG, malignant glioma.

Techniques Used: DNA Methylation Assay, Methylation, Expressing

Analysis of the IRAK3 -related signaling pathway. ( A ) The top 7 signaling pathways with the highest and lowest correlation with glioma. ( B ) The top 30 signaling pathway–related genes enriched in the glioma; the MAPK signaling pathway was highly expressed. ( C ) The MAPK signaling pathway was activated in glioma. ( D ) The status of the top 16 enriched signaling pathways in glioma. Abbreviation: MG, malignant glioma.
Figure Legend Snippet: Analysis of the IRAK3 -related signaling pathway. ( A ) The top 7 signaling pathways with the highest and lowest correlation with glioma. ( B ) The top 30 signaling pathway–related genes enriched in the glioma; the MAPK signaling pathway was highly expressed. ( C ) The MAPK signaling pathway was activated in glioma. ( D ) The status of the top 16 enriched signaling pathways in glioma. Abbreviation: MG, malignant glioma.

Techniques Used:

Analysis of the IRAK3 -related gene expression level. ( A ) Based on the differential genes in the disease data, the distribution of upregulated genes and downregulated genes in the GO-related pathway was enriched. ( B ) The expression level of top 40 MAPK signaling pathway–related genes. Abbreviation: MG, malignant glioma.
Figure Legend Snippet: Analysis of the IRAK3 -related gene expression level. ( A ) Based on the differential genes in the disease data, the distribution of upregulated genes and downregulated genes in the GO-related pathway was enriched. ( B ) The expression level of top 40 MAPK signaling pathway–related genes. Abbreviation: MG, malignant glioma.

Techniques Used: Expressing

Methylation level and expression level of the IRAK3 in glioma tissues and cells. ( A ) The IRAK3 was highly methylated in glioma tissues compared with adjacent tissues. ( B ) The IRAK3 was less expressed in glioma tissues than in adjacent tissues. ( C ) The IRAK3 in the five glioma cells (SHG-44, U251, HS683, SF-539, and GOS-3) was hypermethylated compared with U251 glioma cells. ( D ) The IRAK3 was less expressed in the five glioma cells than in normal glioma cells. The difference was significant (** P < 0.01).
Figure Legend Snippet: Methylation level and expression level of the IRAK3 in glioma tissues and cells. ( A ) The IRAK3 was highly methylated in glioma tissues compared with adjacent tissues. ( B ) The IRAK3 was less expressed in glioma tissues than in adjacent tissues. ( C ) The IRAK3 in the five glioma cells (SHG-44, U251, HS683, SF-539, and GOS-3) was hypermethylated compared with U251 glioma cells. ( D ) The IRAK3 was less expressed in the five glioma cells than in normal glioma cells. The difference was significant (** P < 0.01).

Techniques Used: Methylation, Expressing

Effect on glioma cells after the overexpression and demethylation of the IRAK3 . ( A ) The expression level of the IRAK3 was significantly higher in the glioma U251 cells of the pcDNA3.1-IRAK3 and 5-aza-dC groups than in the control group. ( B and C ) The protein expression level of the IRAK3 significantly increased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( D ) The expression level of the inflammatory factor IL-6 significantly decreased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( E ) The activity significantly decreased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( F and G ) The apoptosis rate significantly increased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( H and I ) The migration capability was significantly lower in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( J and K ) The invasiveness capability was significantly lower in the pcDNA3.1-IRAK3 and 5-aza-dC groups than in the the control group. All the mentioned differences were significant (** P < 0.01). The IRAK3 had a suppressive effect on glioma cells in vitro.
Figure Legend Snippet: Effect on glioma cells after the overexpression and demethylation of the IRAK3 . ( A ) The expression level of the IRAK3 was significantly higher in the glioma U251 cells of the pcDNA3.1-IRAK3 and 5-aza-dC groups than in the control group. ( B and C ) The protein expression level of the IRAK3 significantly increased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( D ) The expression level of the inflammatory factor IL-6 significantly decreased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( E ) The activity significantly decreased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( F and G ) The apoptosis rate significantly increased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( H and I ) The migration capability was significantly lower in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( J and K ) The invasiveness capability was significantly lower in the pcDNA3.1-IRAK3 and 5-aza-dC groups than in the the control group. All the mentioned differences were significant (** P < 0.01). The IRAK3 had a suppressive effect on glioma cells in vitro.

Techniques Used: Over Expression, Expressing, Activity Assay, Migration, In Vitro

Effect on glioma cells after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A ) The expression level of MAPK signaling pathway–related genes expression level was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( B and C ) The expression level of MAPK signaling pathway–related proteins significantly decreased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. ( D ) The level of inflammatory factor IL-6 was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( E ) The activity significantly decreased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. ( F and G ) The apoptosis rate significantly increased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. All the mentioned differences were significant (* P < 0.05, ** P < 0.01).
Figure Legend Snippet: Effect on glioma cells after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A ) The expression level of MAPK signaling pathway–related genes expression level was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( B and C ) The expression level of MAPK signaling pathway–related proteins significantly decreased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. ( D ) The level of inflammatory factor IL-6 was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( E ) The activity significantly decreased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. ( F and G ) The apoptosis rate significantly increased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. All the mentioned differences were significant (* P < 0.05, ** P < 0.01).

Techniques Used: Expressing, Activity Assay

Effect on the migration and invasiveness capability of glioma cells after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A and B ) The migration capability was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( A and C ) The invasiveness capability was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. All the mentioned differences were significant (** P < 0.01).
Figure Legend Snippet: Effect on the migration and invasiveness capability of glioma cells after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A and B ) The migration capability was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( A and C ) The invasiveness capability was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. All the mentioned differences were significant (** P < 0.01).

Techniques Used: Migration

Effect on mice after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A ) The tumor in the transplantation PD325901, p- IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups after 7, 14, 21, and 28 days. ( B ) The tumor volume was smaller in the PD325901, pcDNA3.1-IRAK3+PD325901, and 5-aza-dC+PD325901 groups compared with the control group. ( C ) The tumor weight followed the same trend as the volume. ( D ) The expression level of the IRAK3 was lower in the transplantation PD325901, pcDNA3.1-IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups compared with the transplantation control group. ( E and F ) The protein expression level of IRAK3 was lower in the transplantation PD325901, pcDNA3.1-IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups than in the transplantation control group. All the mentioned differences were significant (* P < 0.05, ** P < 0.01).
Figure Legend Snippet: Effect on mice after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A ) The tumor in the transplantation PD325901, p- IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups after 7, 14, 21, and 28 days. ( B ) The tumor volume was smaller in the PD325901, pcDNA3.1-IRAK3+PD325901, and 5-aza-dC+PD325901 groups compared with the control group. ( C ) The tumor weight followed the same trend as the volume. ( D ) The expression level of the IRAK3 was lower in the transplantation PD325901, pcDNA3.1-IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups compared with the transplantation control group. ( E and F ) The protein expression level of IRAK3 was lower in the transplantation PD325901, pcDNA3.1-IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups than in the transplantation control group. All the mentioned differences were significant (* P < 0.05, ** P < 0.01).

Techniques Used: Transplantation Assay, Expressing


Structured Review

Abcam anti irak3 antibody
Anti Irak3 Antibody, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti irak3 antibody/product/Abcam
Average 86 stars, based on 1 article reviews
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anti irak3 antibody - by Bioz Stars, 2024-07
86/100 stars

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Abcam anti mouse monoclonal antibody irak3
Anti Mouse Monoclonal Antibody Irak3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti mouse monoclonal antibody irak3/product/Abcam
Average 86 stars, based on 1 article reviews
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anti mouse monoclonal antibody irak3 - by Bioz Stars, 2024-07
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Abcam anti irak3 antibodies
Anti Irak3 Antibodies, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti irak3 antibodies/product/Abcam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
anti irak3 antibodies - by Bioz Stars, 2024-07
86/100 stars

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Abcam rabbit anti irak3 ab ab8116
Rabbit Anti Irak3 Ab Ab8116, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/rabbit anti irak3 ab ab8116/product/Abcam
Average 86 stars, based on 1 article reviews
Price from $9.99 to $1999.99
rabbit anti irak3 ab ab8116 - by Bioz Stars, 2024-07
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    Abcam anti irak3
    Anti Irak3, supplied by Abcam, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    irak3  (Abcam)
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    Abcam anti irak3 antibody
    qRT-PCR Primers
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    Abcam anti mouse monoclonal antibody irak3
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    qRT-PCR Primers

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: qRT-PCR Primers

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: Sequencing

    Methylation-Specific Primers

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: Methylation-Specific Primers

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: Methylation, Sequencing

    DNA methylation analysis of tumor tissues and normal tissues and analysis of methylation degree for seven CpG sites of IRAK3 . ( A ) The expression of the top 20 candidate genes was analyzed. IRAK3 was hypermethylated significantly in tumor tissues compared with normal tissues. ( B ) The number of IRAK3 –methylated CpG islands in each isosite. ( C – I ) Seven CpG sites for IRAK3 , which are presented in the boxplot, displayed a decreased methylation in the tumor group. The boxplot for cg 26,415,547 ( C ), cg 26,279,550 ( D ), cg 20,162,652 ( E ), cg 18,177,616 ( F ), cg 13,807,985 ( G ), cg 10,389,229 ( H ), and cg 01263292 ( I ). Abbreviation: MG, malignant glioma.

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: DNA methylation analysis of tumor tissues and normal tissues and analysis of methylation degree for seven CpG sites of IRAK3 . ( A ) The expression of the top 20 candidate genes was analyzed. IRAK3 was hypermethylated significantly in tumor tissues compared with normal tissues. ( B ) The number of IRAK3 –methylated CpG islands in each isosite. ( C – I ) Seven CpG sites for IRAK3 , which are presented in the boxplot, displayed a decreased methylation in the tumor group. The boxplot for cg 26,415,547 ( C ), cg 26,279,550 ( D ), cg 20,162,652 ( E ), cg 18,177,616 ( F ), cg 13,807,985 ( G ), cg 10,389,229 ( H ), and cg 01263292 ( I ). Abbreviation: MG, malignant glioma.

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: DNA Methylation Assay, Methylation, Expressing

    Analysis of the IRAK3 -related signaling pathway. ( A ) The top 7 signaling pathways with the highest and lowest correlation with glioma. ( B ) The top 30 signaling pathway–related genes enriched in the glioma; the MAPK signaling pathway was highly expressed. ( C ) The MAPK signaling pathway was activated in glioma. ( D ) The status of the top 16 enriched signaling pathways in glioma. Abbreviation: MG, malignant glioma.

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: Analysis of the IRAK3 -related signaling pathway. ( A ) The top 7 signaling pathways with the highest and lowest correlation with glioma. ( B ) The top 30 signaling pathway–related genes enriched in the glioma; the MAPK signaling pathway was highly expressed. ( C ) The MAPK signaling pathway was activated in glioma. ( D ) The status of the top 16 enriched signaling pathways in glioma. Abbreviation: MG, malignant glioma.

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques:

    Analysis of the IRAK3 -related gene expression level. ( A ) Based on the differential genes in the disease data, the distribution of upregulated genes and downregulated genes in the GO-related pathway was enriched. ( B ) The expression level of top 40 MAPK signaling pathway–related genes. Abbreviation: MG, malignant glioma.

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: Analysis of the IRAK3 -related gene expression level. ( A ) Based on the differential genes in the disease data, the distribution of upregulated genes and downregulated genes in the GO-related pathway was enriched. ( B ) The expression level of top 40 MAPK signaling pathway–related genes. Abbreviation: MG, malignant glioma.

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: Expressing

    Methylation level and expression level of the IRAK3 in glioma tissues and cells. ( A ) The IRAK3 was highly methylated in glioma tissues compared with adjacent tissues. ( B ) The IRAK3 was less expressed in glioma tissues than in adjacent tissues. ( C ) The IRAK3 in the five glioma cells (SHG-44, U251, HS683, SF-539, and GOS-3) was hypermethylated compared with U251 glioma cells. ( D ) The IRAK3 was less expressed in the five glioma cells than in normal glioma cells. The difference was significant (** P < 0.01).

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: Methylation level and expression level of the IRAK3 in glioma tissues and cells. ( A ) The IRAK3 was highly methylated in glioma tissues compared with adjacent tissues. ( B ) The IRAK3 was less expressed in glioma tissues than in adjacent tissues. ( C ) The IRAK3 in the five glioma cells (SHG-44, U251, HS683, SF-539, and GOS-3) was hypermethylated compared with U251 glioma cells. ( D ) The IRAK3 was less expressed in the five glioma cells than in normal glioma cells. The difference was significant (** P < 0.01).

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: Methylation, Expressing

    Effect on glioma cells after the overexpression and demethylation of the IRAK3 . ( A ) The expression level of the IRAK3 was significantly higher in the glioma U251 cells of the pcDNA3.1-IRAK3 and 5-aza-dC groups than in the control group. ( B and C ) The protein expression level of the IRAK3 significantly increased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( D ) The expression level of the inflammatory factor IL-6 significantly decreased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( E ) The activity significantly decreased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( F and G ) The apoptosis rate significantly increased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( H and I ) The migration capability was significantly lower in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( J and K ) The invasiveness capability was significantly lower in the pcDNA3.1-IRAK3 and 5-aza-dC groups than in the the control group. All the mentioned differences were significant (** P < 0.01). The IRAK3 had a suppressive effect on glioma cells in vitro.

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: Effect on glioma cells after the overexpression and demethylation of the IRAK3 . ( A ) The expression level of the IRAK3 was significantly higher in the glioma U251 cells of the pcDNA3.1-IRAK3 and 5-aza-dC groups than in the control group. ( B and C ) The protein expression level of the IRAK3 significantly increased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( D ) The expression level of the inflammatory factor IL-6 significantly decreased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( E ) The activity significantly decreased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( F and G ) The apoptosis rate significantly increased in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( H and I ) The migration capability was significantly lower in the pcDNA3.1-IRAK3 and 5-aza-dC groups compared with the control group. ( J and K ) The invasiveness capability was significantly lower in the pcDNA3.1-IRAK3 and 5-aza-dC groups than in the the control group. All the mentioned differences were significant (** P < 0.01). The IRAK3 had a suppressive effect on glioma cells in vitro.

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: Over Expression, Expressing, Activity Assay, Migration, In Vitro

    Effect on glioma cells after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A ) The expression level of MAPK signaling pathway–related genes expression level was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( B and C ) The expression level of MAPK signaling pathway–related proteins significantly decreased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. ( D ) The level of inflammatory factor IL-6 was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( E ) The activity significantly decreased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. ( F and G ) The apoptosis rate significantly increased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. All the mentioned differences were significant (* P < 0.05, ** P < 0.01).

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: Effect on glioma cells after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A ) The expression level of MAPK signaling pathway–related genes expression level was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( B and C ) The expression level of MAPK signaling pathway–related proteins significantly decreased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. ( D ) The level of inflammatory factor IL-6 was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( E ) The activity significantly decreased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. ( F and G ) The apoptosis rate significantly increased in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups compared with the control group. All the mentioned differences were significant (* P < 0.05, ** P < 0.01).

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: Expressing, Activity Assay

    Effect on the migration and invasiveness capability of glioma cells after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A and B ) The migration capability was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( A and C ) The invasiveness capability was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. All the mentioned differences were significant (** P < 0.01).

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: Effect on the migration and invasiveness capability of glioma cells after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A and B ) The migration capability was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. ( A and C ) The invasiveness capability was significantly lower in the PD325901, pcDNA3.1-IRAK3, and 5-aza-dC groups than in the control group. All the mentioned differences were significant (** P < 0.01).

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: Migration

    Effect on mice after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A ) The tumor in the transplantation PD325901, p- IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups after 7, 14, 21, and 28 days. ( B ) The tumor volume was smaller in the PD325901, pcDNA3.1-IRAK3+PD325901, and 5-aza-dC+PD325901 groups compared with the control group. ( C ) The tumor weight followed the same trend as the volume. ( D ) The expression level of the IRAK3 was lower in the transplantation PD325901, pcDNA3.1-IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups compared with the transplantation control group. ( E and F ) The protein expression level of IRAK3 was lower in the transplantation PD325901, pcDNA3.1-IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups than in the transplantation control group. All the mentioned differences were significant (* P < 0.05, ** P < 0.01).

    Journal: Cancer Management and Research

    Article Title: Hypermethylation of the IRAK3 -Activated MAPK Signaling Pathway to Promote the Development of Glioma

    doi: 10.2147/CMAR.S252772

    Figure Lengend Snippet: Effect on mice after treatment with the MAPK signaling pathway inhibitor PD325901, pcDNA3.1-IRAK3, and 5-aza-dC. ( A ) The tumor in the transplantation PD325901, p- IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups after 7, 14, 21, and 28 days. ( B ) The tumor volume was smaller in the PD325901, pcDNA3.1-IRAK3+PD325901, and 5-aza-dC+PD325901 groups compared with the control group. ( C ) The tumor weight followed the same trend as the volume. ( D ) The expression level of the IRAK3 was lower in the transplantation PD325901, pcDNA3.1-IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups compared with the transplantation control group. ( E and F ) The protein expression level of IRAK3 was lower in the transplantation PD325901, pcDNA3.1-IRAK3 + PD325901, and 5-aza-dC + PD325901 treatment groups than in the transplantation control group. All the mentioned differences were significant (* P < 0.05, ** P < 0.01).

    Article Snippet: The membranes were probed with primary anti- IRAK3 antibody (ab8116, Abcam, MA, USA), anti- MEK1 (phospho S298) antibody [EPR3338] (ab96379), anti- ERK1 + ERK2 antibody [ERK-7D8] (ab54230), anti-c- Fos (phospho T232) antibody (ab17933), and anti- GAPDH antibody [6C5] (ab8245, Abcam) as control.

    Techniques: Transplantation Assay, Expressing