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Bio-Rad iq sybr green supermix
Iq Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 3038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 90 stars, based on 3038 article reviews
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iq sybr green supermix - by Bioz Stars, 2020-01
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Amplification:

Article Title: Impairment of Pseudomonas aeruginosa Biofilm Resistance to Antibiotics by Combining the Drugs with a New Quorum-Sensing Inhibitor
Article Snippet: .. The primer sequences were subjected to a BLAST analysis against the P. aeruginosa PAO1 genome sequence to eliminate the possibility of nonspecific binding. qPCR was carried out using the IQ SYBR green supermix (Bio-Rad, Marnes-la-Coquette, France) with a 25-μl reaction mixture containing 12.5 μl of 2× IQ SYBR green supermix, 0.2 μM each forward and reverse primer, and 5 μl of extracted DNA. qPCR was performed using an iCycler IQ5 real-time PCR detection system (Bio-Rad, Marnes-la-Coquette, France) and the following cycling parameters: an initial denaturation at 95°C for 3 min, followed by 50 cycles of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. Depending on the melting temperature ( Tm ) of a specific amplicon, the melt curve identifies a characteristic Tm that can distinguish between amplicons differing by only a single base. .. For our study, a melt curve analysis was performed immediately after amplification at 95°C for 1 min and 55°C for 1 min, followed by 81 repeats of heating for 10 s starting at 55°C and increasing in 0.5°C increments.

Synthesized:

Article Title: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex
Article Snippet: The first-strand complementary DNA (cDNA) was synthesized using M-MLV reverse transcriptase (Promega, Cat. number M1705) in a total volume of 20 μ L reaction system containing 2 μ g total RNA. .. The PCR reaction mixtures contained 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad, Cat. number 1708882AP), 0.5 μ M primers, and 0.8 μ L of cDNA.

Quantitative RT-PCR:

Article Title: Rye (Secale cereale) supernumerary (B) chromosomes associated with heat tolerance during early stages of male sporogenesis
Article Snippet: .. For transcript quantification, cDNA from leaves and staged anthers from 0B and 2B plants was analysed by qRT-PCR with the Bio-Rad IQ SYBR Green Supermix (cat. no. 170-8880S). .. PCRs were conducted as in with gene- or sequence-specific primers.

Article Title: Perinatal Bisphenol A Exposure and Reprogramming of Imprinted Gene Expression in the Adult Mouse Brain
Article Snippet: .. See for primer sequences; 1000 ng of RNA from each sample was reverse transcribed into complementary DNA (cDNA) using the Bio-Rad iScript cDNA Synthesis Kit (Cat. #1708890). cDNA was diluted 1:5 in RNase-free water, mixed with 10 µM forward/reverse primers, nuclease-free water, and Bio-Rad iQ SYBR Green Supermix (Cat. #1708880). qRT-PCR was performed using the pre-programmed 2-step PCR+ melt curve protocol on a Bio-rad CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad; Hercules, CA). ..

Article Title: Overexpression of the Soybean NAC Gene GmNAC109 Increases Lateral Root Formation and Abiotic Stress Tolerance in Transgenic Arabidopsis Plants
Article Snippet: .. Approximately 1 μg of total RNA was reverse-transcribed using an iScript™ cDNA Synthesis Kit (Cat. 1708891; Bio-Rad, CA, USA). qRT-PCR was performed using iQ™ SYBR® Green Supermix (Cat. 170-8880AP; Bio-Rad). .. PCR was conducted on a LightCycler 480 Real-Time PCR system (Roche Diagnostics, Laval, QC, Canada).

Article Title: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex
Article Snippet: The PCR reaction mixtures contained 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad, Cat. number 1708882AP), 0.5 μ M primers, and 0.8 μ L of cDNA. .. The CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) was used to run qRT-PCR reaction.

SYBR Green Assay:

Article Title: Rye (Secale cereale) supernumerary (B) chromosomes associated with heat tolerance during early stages of male sporogenesis
Article Snippet: .. For transcript quantification, cDNA from leaves and staged anthers from 0B and 2B plants was analysed by qRT-PCR with the Bio-Rad IQ SYBR Green Supermix (cat. no. 170-8880S). .. PCRs were conducted as in with gene- or sequence-specific primers.

Article Title: Perinatal Bisphenol A Exposure and Reprogramming of Imprinted Gene Expression in the Adult Mouse Brain
Article Snippet: .. See for primer sequences; 1000 ng of RNA from each sample was reverse transcribed into complementary DNA (cDNA) using the Bio-Rad iScript cDNA Synthesis Kit (Cat. #1708890). cDNA was diluted 1:5 in RNase-free water, mixed with 10 µM forward/reverse primers, nuclease-free water, and Bio-Rad iQ SYBR Green Supermix (Cat. #1708880). qRT-PCR was performed using the pre-programmed 2-step PCR+ melt curve protocol on a Bio-rad CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad; Hercules, CA). ..

Article Title: Epigenetic reprogramming converts human Wharton’s jelly mesenchymal stem cells into functional cardiomyocytes by differential regulation of Wnt mediators
Article Snippet: .. Real-time quantitative reverse-transcription PCR The qualitative results of mRNA expression were further quantified using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) in a real-time PCR system. cDNAs and gene-specific primers were mixed with 2× iQ SYBR Green Supermix (Bio-Rad), and dispensed on a MicroAmp® Optical 8- Tube Strip. .. Fluorescence shift was observed using a 7500 Real-time PCR system (Applied Biosystems, Carlsbad, CA, USA).

Article Title: Overexpression of the Soybean NAC Gene GmNAC109 Increases Lateral Root Formation and Abiotic Stress Tolerance in Transgenic Arabidopsis Plants
Article Snippet: .. Approximately 1 μg of total RNA was reverse-transcribed using an iScript™ cDNA Synthesis Kit (Cat. 1708891; Bio-Rad, CA, USA). qRT-PCR was performed using iQ™ SYBR® Green Supermix (Cat. 170-8880AP; Bio-Rad). .. PCR was conducted on a LightCycler 480 Real-Time PCR system (Roche Diagnostics, Laval, QC, Canada).

Article Title: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex
Article Snippet: .. The PCR reaction mixtures contained 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad, Cat. number 1708882AP), 0.5 μ M primers, and 0.8 μ L of cDNA. .. The CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) was used to run qRT-PCR reaction.

Article Title: Impairment of Pseudomonas aeruginosa Biofilm Resistance to Antibiotics by Combining the Drugs with a New Quorum-Sensing Inhibitor
Article Snippet: .. The primer sequences were subjected to a BLAST analysis against the P. aeruginosa PAO1 genome sequence to eliminate the possibility of nonspecific binding. qPCR was carried out using the IQ SYBR green supermix (Bio-Rad, Marnes-la-Coquette, France) with a 25-μl reaction mixture containing 12.5 μl of 2× IQ SYBR green supermix, 0.2 μM each forward and reverse primer, and 5 μl of extracted DNA. qPCR was performed using an iCycler IQ5 real-time PCR detection system (Bio-Rad, Marnes-la-Coquette, France) and the following cycling parameters: an initial denaturation at 95°C for 3 min, followed by 50 cycles of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. Depending on the melting temperature ( Tm ) of a specific amplicon, the melt curve identifies a characteristic Tm that can distinguish between amplicons differing by only a single base. .. For our study, a melt curve analysis was performed immediately after amplification at 95°C for 1 min and 55°C for 1 min, followed by 81 repeats of heating for 10 s starting at 55°C and increasing in 0.5°C increments.

Article Title: A novel, multiplex, real-time PCR-based approach for the detection of the commonly occurring pathogenic fungi and bacteria
Article Snippet: .. The tested dyes were LCGreen “LightCycler® 480 High Resolution Melting Master” (Roche Diagnostic GmbH, Mannheim, Germany); SybrGreen “LightCycler® 480 DNA Master SYBR Green I”, (Roche); “IQ™ SYBR® Green Supermix” (Bio-Rad Laboratries, Inc., Hercules, CA, USA); “Maxima™ SYBR Green qPCR Master Mix no ROX” (Fermentas, Vilnius, Lithuania); and EvaGreen (“LC-FastStart DNA Master Hybridization Probes” (Roche) combined with EvaGreen dye (Biotium Inc., Hayward, CA, USA) and “Sso Fast™ EvaGreen® Supermix” (BioRad). ..

Article Title: A functional microRNA screening method for organ morphogenesis
Article Snippet: .. 720001180) iQ™ SYBR® Green Supermix (BIORAD, cat.no. .. 170-8880) miScript II RT Kit (QIAGEN, cat.no.

Stripping Membranes:

Article Title: Epigenetic reprogramming converts human Wharton’s jelly mesenchymal stem cells into functional cardiomyocytes by differential regulation of Wnt mediators
Article Snippet: .. Real-time quantitative reverse-transcription PCR The qualitative results of mRNA expression were further quantified using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) in a real-time PCR system. cDNAs and gene-specific primers were mixed with 2× iQ SYBR Green Supermix (Bio-Rad), and dispensed on a MicroAmp® Optical 8- Tube Strip. .. Fluorescence shift was observed using a 7500 Real-time PCR system (Applied Biosystems, Carlsbad, CA, USA).

Infection:

Article Title: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex
Article Snippet: RNA Extraction and Real-Time PCR Analysis The total RNA was collected from 95D and A549 cells after 5 and 8 days of lentivirus infection, respectively, by using TRIzol® reagent (Life Technologies, Cat. number 15596-018). .. The PCR reaction mixtures contained 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad, Cat. number 1708882AP), 0.5 μ M primers, and 0.8 μ L of cDNA.

Expressing:

Article Title: Perinatal Bisphenol A Exposure and Reprogramming of Imprinted Gene Expression in the Adult Mouse Brain
Article Snippet: Paragraph title: Analysis of Gene Expression Using Quantitative Real-Time PCR ... See for primer sequences; 1000 ng of RNA from each sample was reverse transcribed into complementary DNA (cDNA) using the Bio-Rad iScript cDNA Synthesis Kit (Cat. #1708890). cDNA was diluted 1:5 in RNase-free water, mixed with 10 µM forward/reverse primers, nuclease-free water, and Bio-Rad iQ SYBR Green Supermix (Cat. #1708880). qRT-PCR was performed using the pre-programmed 2-step PCR+ melt curve protocol on a Bio-rad CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad; Hercules, CA).

Article Title: Epigenetic reprogramming converts human Wharton’s jelly mesenchymal stem cells into functional cardiomyocytes by differential regulation of Wnt mediators
Article Snippet: .. Real-time quantitative reverse-transcription PCR The qualitative results of mRNA expression were further quantified using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) in a real-time PCR system. cDNAs and gene-specific primers were mixed with 2× iQ SYBR Green Supermix (Bio-Rad), and dispensed on a MicroAmp® Optical 8- Tube Strip. .. Fluorescence shift was observed using a 7500 Real-time PCR system (Applied Biosystems, Carlsbad, CA, USA).

Article Title: Overexpression of the Soybean NAC Gene GmNAC109 Increases Lateral Root Formation and Abiotic Stress Tolerance in Transgenic Arabidopsis Plants
Article Snippet: Quantitative Real-Time PCR (qRT-PCR) To examine GmNAC109 expression in soybean under different stresses and stress-related genes in Arabidopsis transgenic lines, total RNA was extracted using a GeneAll Ribospin™ Plant Kit (Cat. 307-150; GeneAll, Seoul, Korea). .. Approximately 1 μg of total RNA was reverse-transcribed using an iScript™ cDNA Synthesis Kit (Cat. 1708891; Bio-Rad, CA, USA). qRT-PCR was performed using iQ™ SYBR® Green Supermix (Cat. 170-8880AP; Bio-Rad).

Article Title: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex
Article Snippet: The PCR reaction mixtures contained 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad, Cat. number 1708882AP), 0.5 μ M primers, and 0.8 μ L of cDNA. .. The PCR reaction mixture was run as follows: initial denaturation at 95°C for 1 min, 40 cycles of denaturation at 95°C for 5 s, and extension at 60°C for 20 s. The relative gene expression level was calculated using the 2−ΔΔCT method with normalization to the internal control β -actin.

Article Title: A functional microRNA screening method for organ morphogenesis
Article Snippet: 720001180) iQ™ SYBR® Green Supermix (BIORAD, cat.no. .. 218073) qPCR primers: Mature miRNA expression: miScript Primer Assays (QIAGEN) miRNA targets and other genes (Invitrogen primers)

Hybridization:

Article Title: A novel, multiplex, real-time PCR-based approach for the detection of the commonly occurring pathogenic fungi and bacteria
Article Snippet: .. The tested dyes were LCGreen “LightCycler® 480 High Resolution Melting Master” (Roche Diagnostic GmbH, Mannheim, Germany); SybrGreen “LightCycler® 480 DNA Master SYBR Green I”, (Roche); “IQ™ SYBR® Green Supermix” (Bio-Rad Laboratries, Inc., Hercules, CA, USA); “Maxima™ SYBR Green qPCR Master Mix no ROX” (Fermentas, Vilnius, Lithuania); and EvaGreen (“LC-FastStart DNA Master Hybridization Probes” (Roche) combined with EvaGreen dye (Biotium Inc., Hayward, CA, USA) and “Sso Fast™ EvaGreen® Supermix” (BioRad). ..

Sequencing:

Article Title: Rye (Secale cereale) supernumerary (B) chromosomes associated with heat tolerance during early stages of male sporogenesis
Article Snippet: For transcript quantification, cDNA from leaves and staged anthers from 0B and 2B plants was analysed by qRT-PCR with the Bio-Rad IQ SYBR Green Supermix (cat. no. 170-8880S). .. PCRs were conducted as in with gene- or sequence-specific primers.

Article Title: Impairment of Pseudomonas aeruginosa Biofilm Resistance to Antibiotics by Combining the Drugs with a New Quorum-Sensing Inhibitor
Article Snippet: .. The primer sequences were subjected to a BLAST analysis against the P. aeruginosa PAO1 genome sequence to eliminate the possibility of nonspecific binding. qPCR was carried out using the IQ SYBR green supermix (Bio-Rad, Marnes-la-Coquette, France) with a 25-μl reaction mixture containing 12.5 μl of 2× IQ SYBR green supermix, 0.2 μM each forward and reverse primer, and 5 μl of extracted DNA. qPCR was performed using an iCycler IQ5 real-time PCR detection system (Bio-Rad, Marnes-la-Coquette, France) and the following cycling parameters: an initial denaturation at 95°C for 3 min, followed by 50 cycles of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. Depending on the melting temperature ( Tm ) of a specific amplicon, the melt curve identifies a characteristic Tm that can distinguish between amplicons differing by only a single base. .. For our study, a melt curve analysis was performed immediately after amplification at 95°C for 1 min and 55°C for 1 min, followed by 81 repeats of heating for 10 s starting at 55°C and increasing in 0.5°C increments.

Transferring:

Article Title: A functional microRNA screening method for organ morphogenesis
Article Snippet: N2913-1ML) Antagomirs and Biotinylated Mimics (QIAGEN and Dharmacon) Off-target miR-Cy3 microRNA (QIAGEN) 0.7 mL sterile plastic tubes (Eppendorf or similar) Rainin Pipet Plus (Rainin, P1000, P200, and P10) Sterile Filter Pipette Tips (1000 μL, 200 μL, 10 μL) Fetal Bovine Serum (Stasis Stem Cells, cat.no. .. 720001180) iQ™ SYBR® Green Supermix (BIORAD, cat.no.

Cell Culture:

Article Title: Impairment of Pseudomonas aeruginosa Biofilm Resistance to Antibiotics by Combining the Drugs with a New Quorum-Sensing Inhibitor
Article Snippet: The primer sequences were subjected to a BLAST analysis against the P. aeruginosa PAO1 genome sequence to eliminate the possibility of nonspecific binding. qPCR was carried out using the IQ SYBR green supermix (Bio-Rad, Marnes-la-Coquette, France) with a 25-μl reaction mixture containing 12.5 μl of 2× IQ SYBR green supermix, 0.2 μM each forward and reverse primer, and 5 μl of extracted DNA. qPCR was performed using an iCycler IQ5 real-time PCR detection system (Bio-Rad, Marnes-la-Coquette, France) and the following cycling parameters: an initial denaturation at 95°C for 3 min, followed by 50 cycles of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. Depending on the melting temperature ( Tm ) of a specific amplicon, the melt curve identifies a characteristic Tm that can distinguish between amplicons differing by only a single base. .. In order to construct standard curves, three control biofilms were scraped and the number of CFU per square centimeter of each biofilm was determined by cell culture.

Polymerase Chain Reaction:

Article Title: Perinatal Bisphenol A Exposure and Reprogramming of Imprinted Gene Expression in the Adult Mouse Brain
Article Snippet: .. See for primer sequences; 1000 ng of RNA from each sample was reverse transcribed into complementary DNA (cDNA) using the Bio-Rad iScript cDNA Synthesis Kit (Cat. #1708890). cDNA was diluted 1:5 in RNase-free water, mixed with 10 µM forward/reverse primers, nuclease-free water, and Bio-Rad iQ SYBR Green Supermix (Cat. #1708880). qRT-PCR was performed using the pre-programmed 2-step PCR+ melt curve protocol on a Bio-rad CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad; Hercules, CA). ..

Article Title: Epigenetic reprogramming converts human Wharton’s jelly mesenchymal stem cells into functional cardiomyocytes by differential regulation of Wnt mediators
Article Snippet: .. Real-time quantitative reverse-transcription PCR The qualitative results of mRNA expression were further quantified using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) in a real-time PCR system. cDNAs and gene-specific primers were mixed with 2× iQ SYBR Green Supermix (Bio-Rad), and dispensed on a MicroAmp® Optical 8- Tube Strip. .. Fluorescence shift was observed using a 7500 Real-time PCR system (Applied Biosystems, Carlsbad, CA, USA).

Article Title: Overexpression of the Soybean NAC Gene GmNAC109 Increases Lateral Root Formation and Abiotic Stress Tolerance in Transgenic Arabidopsis Plants
Article Snippet: Approximately 1 μg of total RNA was reverse-transcribed using an iScript™ cDNA Synthesis Kit (Cat. 1708891; Bio-Rad, CA, USA). qRT-PCR was performed using iQ™ SYBR® Green Supermix (Cat. 170-8880AP; Bio-Rad). .. PCR was conducted on a LightCycler 480 Real-Time PCR system (Roche Diagnostics, Laval, QC, Canada).

Article Title: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex
Article Snippet: .. The PCR reaction mixtures contained 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad, Cat. number 1708882AP), 0.5 μ M primers, and 0.8 μ L of cDNA. .. The CFX96 Touch™ Real-Time PCR Detection System (Bio-Rad) was used to run qRT-PCR reaction.

Article Title: A functional microRNA screening method for organ morphogenesis
Article Snippet: 720001180) iQ™ SYBR® Green Supermix (BIORAD, cat.no. .. 218160) miScript SYBR® Green PCR Kit (QIAGEN, cat.no.

Binding Assay:

Article Title: Impairment of Pseudomonas aeruginosa Biofilm Resistance to Antibiotics by Combining the Drugs with a New Quorum-Sensing Inhibitor
Article Snippet: .. The primer sequences were subjected to a BLAST analysis against the P. aeruginosa PAO1 genome sequence to eliminate the possibility of nonspecific binding. qPCR was carried out using the IQ SYBR green supermix (Bio-Rad, Marnes-la-Coquette, France) with a 25-μl reaction mixture containing 12.5 μl of 2× IQ SYBR green supermix, 0.2 μM each forward and reverse primer, and 5 μl of extracted DNA. qPCR was performed using an iCycler IQ5 real-time PCR detection system (Bio-Rad, Marnes-la-Coquette, France) and the following cycling parameters: an initial denaturation at 95°C for 3 min, followed by 50 cycles of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. Depending on the melting temperature ( Tm ) of a specific amplicon, the melt curve identifies a characteristic Tm that can distinguish between amplicons differing by only a single base. .. For our study, a melt curve analysis was performed immediately after amplification at 95°C for 1 min and 55°C for 1 min, followed by 81 repeats of heating for 10 s starting at 55°C and increasing in 0.5°C increments.

Fluorescence:

Article Title: Epigenetic reprogramming converts human Wharton’s jelly mesenchymal stem cells into functional cardiomyocytes by differential regulation of Wnt mediators
Article Snippet: Real-time quantitative reverse-transcription PCR The qualitative results of mRNA expression were further quantified using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) in a real-time PCR system. cDNAs and gene-specific primers were mixed with 2× iQ SYBR Green Supermix (Bio-Rad), and dispensed on a MicroAmp® Optical 8- Tube Strip. .. Fluorescence shift was observed using a 7500 Real-time PCR system (Applied Biosystems, Carlsbad, CA, USA).

Isolation:

Article Title: Rye (Secale cereale) supernumerary (B) chromosomes associated with heat tolerance during early stages of male sporogenesis
Article Snippet: Paragraph title: cDNA isolation and quantitative PCR ... For transcript quantification, cDNA from leaves and staged anthers from 0B and 2B plants was analysed by qRT-PCR with the Bio-Rad IQ SYBR Green Supermix (cat. no. 170-8880S).

Article Title: A functional microRNA screening method for organ morphogenesis
Article Snippet: 100–125, or similar reagents) mirVana™ total RNA Isolation Kit, with Phenol (AMBION, cat.no. .. 720001180) iQ™ SYBR® Green Supermix (BIORAD, cat.no.

Mouse Assay:

Article Title: Perinatal Bisphenol A Exposure and Reprogramming of Imprinted Gene Expression in the Adult Mouse Brain
Article Snippet: Analysis of Gene Expression Using Quantitative Real-Time PCR Expression of three imprinted genes previously identified to be differentially hydroxymethylated with perinatal BPA exposure ( )—Kcnq1 , Pde10a , and Ppp1r9a and three genes encoding enzymes that catalyze the formation of 5mC and 5hmC—Dnmt1 , Tet1 and Tet2 were quantified separately by brain region for each of the twelve mice using quantitative real-time PCR (qRT-PCR). qRT-PCR primers were designed using the online Genscript Real-time PCR Primer Design software, and specificity for all designed primers was checked using the NCBI Primer-BLAST online tool. .. See for primer sequences; 1000 ng of RNA from each sample was reverse transcribed into complementary DNA (cDNA) using the Bio-Rad iScript cDNA Synthesis Kit (Cat. #1708890). cDNA was diluted 1:5 in RNase-free water, mixed with 10 µM forward/reverse primers, nuclease-free water, and Bio-Rad iQ SYBR Green Supermix (Cat. #1708880). qRT-PCR was performed using the pre-programmed 2-step PCR+ melt curve protocol on a Bio-rad CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad; Hercules, CA).

Transgenic Assay:

Article Title: Overexpression of the Soybean NAC Gene GmNAC109 Increases Lateral Root Formation and Abiotic Stress Tolerance in Transgenic Arabidopsis Plants
Article Snippet: Quantitative Real-Time PCR (qRT-PCR) To examine GmNAC109 expression in soybean under different stresses and stress-related genes in Arabidopsis transgenic lines, total RNA was extracted using a GeneAll Ribospin™ Plant Kit (Cat. 307-150; GeneAll, Seoul, Korea). .. Approximately 1 μg of total RNA was reverse-transcribed using an iScript™ cDNA Synthesis Kit (Cat. 1708891; Bio-Rad, CA, USA). qRT-PCR was performed using iQ™ SYBR® Green Supermix (Cat. 170-8880AP; Bio-Rad).

Construct:

Article Title: Impairment of Pseudomonas aeruginosa Biofilm Resistance to Antibiotics by Combining the Drugs with a New Quorum-Sensing Inhibitor
Article Snippet: The primer sequences were subjected to a BLAST analysis against the P. aeruginosa PAO1 genome sequence to eliminate the possibility of nonspecific binding. qPCR was carried out using the IQ SYBR green supermix (Bio-Rad, Marnes-la-Coquette, France) with a 25-μl reaction mixture containing 12.5 μl of 2× IQ SYBR green supermix, 0.2 μM each forward and reverse primer, and 5 μl of extracted DNA. qPCR was performed using an iCycler IQ5 real-time PCR detection system (Bio-Rad, Marnes-la-Coquette, France) and the following cycling parameters: an initial denaturation at 95°C for 3 min, followed by 50 cycles of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. Depending on the melting temperature ( Tm ) of a specific amplicon, the melt curve identifies a characteristic Tm that can distinguish between amplicons differing by only a single base. .. In order to construct standard curves, three control biofilms were scraped and the number of CFU per square centimeter of each biofilm was determined by cell culture.

Chloramphenicol Acetyltransferase Assay:

Article Title: A functional microRNA screening method for organ morphogenesis
Article Snippet: .. 720001180) iQ™ SYBR® Green Supermix (BIORAD, cat.no. .. 170-8880) miScript II RT Kit (QIAGEN, cat.no.

Software:

Article Title: Perinatal Bisphenol A Exposure and Reprogramming of Imprinted Gene Expression in the Adult Mouse Brain
Article Snippet: Analysis of Gene Expression Using Quantitative Real-Time PCR Expression of three imprinted genes previously identified to be differentially hydroxymethylated with perinatal BPA exposure ( )—Kcnq1 , Pde10a , and Ppp1r9a and three genes encoding enzymes that catalyze the formation of 5mC and 5hmC—Dnmt1 , Tet1 and Tet2 were quantified separately by brain region for each of the twelve mice using quantitative real-time PCR (qRT-PCR). qRT-PCR primers were designed using the online Genscript Real-time PCR Primer Design software, and specificity for all designed primers was checked using the NCBI Primer-BLAST online tool. .. See for primer sequences; 1000 ng of RNA from each sample was reverse transcribed into complementary DNA (cDNA) using the Bio-Rad iScript cDNA Synthesis Kit (Cat. #1708890). cDNA was diluted 1:5 in RNase-free water, mixed with 10 µM forward/reverse primers, nuclease-free water, and Bio-Rad iQ SYBR Green Supermix (Cat. #1708880). qRT-PCR was performed using the pre-programmed 2-step PCR+ melt curve protocol on a Bio-rad CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad; Hercules, CA).

Article Title: Impairment of Pseudomonas aeruginosa Biofilm Resistance to Antibiotics by Combining the Drugs with a New Quorum-Sensing Inhibitor
Article Snippet: The primers were designed using Primer Express (version 3.0) software (Applied Biosystems, Foster City, CA, USA), and the sequences were as follows: for the rhlR forward primer, 5′-AACGCGAGATCCTGCAATG-3′, and for the rhlR reverse primer, 5′-GCGCGTCGAACTTCTTCTG-3′. .. The primer sequences were subjected to a BLAST analysis against the P. aeruginosa PAO1 genome sequence to eliminate the possibility of nonspecific binding. qPCR was carried out using the IQ SYBR green supermix (Bio-Rad, Marnes-la-Coquette, France) with a 25-μl reaction mixture containing 12.5 μl of 2× IQ SYBR green supermix, 0.2 μM each forward and reverse primer, and 5 μl of extracted DNA. qPCR was performed using an iCycler IQ5 real-time PCR detection system (Bio-Rad, Marnes-la-Coquette, France) and the following cycling parameters: an initial denaturation at 95°C for 3 min, followed by 50 cycles of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. Depending on the melting temperature ( Tm ) of a specific amplicon, the melt curve identifies a characteristic Tm that can distinguish between amplicons differing by only a single base.

Real-time Polymerase Chain Reaction:

Article Title: Rye (Secale cereale) supernumerary (B) chromosomes associated with heat tolerance during early stages of male sporogenesis
Article Snippet: Paragraph title: cDNA isolation and quantitative PCR ... For transcript quantification, cDNA from leaves and staged anthers from 0B and 2B plants was analysed by qRT-PCR with the Bio-Rad IQ SYBR Green Supermix (cat. no. 170-8880S).

Article Title: Perinatal Bisphenol A Exposure and Reprogramming of Imprinted Gene Expression in the Adult Mouse Brain
Article Snippet: Paragraph title: Analysis of Gene Expression Using Quantitative Real-Time PCR ... See for primer sequences; 1000 ng of RNA from each sample was reverse transcribed into complementary DNA (cDNA) using the Bio-Rad iScript cDNA Synthesis Kit (Cat. #1708890). cDNA was diluted 1:5 in RNase-free water, mixed with 10 µM forward/reverse primers, nuclease-free water, and Bio-Rad iQ SYBR Green Supermix (Cat. #1708880). qRT-PCR was performed using the pre-programmed 2-step PCR+ melt curve protocol on a Bio-rad CFX96 Real-Time System C1000 Thermal Cycler (Bio-Rad; Hercules, CA).

Article Title: Epigenetic reprogramming converts human Wharton’s jelly mesenchymal stem cells into functional cardiomyocytes by differential regulation of Wnt mediators
Article Snippet: .. Real-time quantitative reverse-transcription PCR The qualitative results of mRNA expression were further quantified using iQ SYBR Green Supermix (Bio-Rad, Hercules, CA, USA) in a real-time PCR system. cDNAs and gene-specific primers were mixed with 2× iQ SYBR Green Supermix (Bio-Rad), and dispensed on a MicroAmp® Optical 8- Tube Strip. .. Fluorescence shift was observed using a 7500 Real-time PCR system (Applied Biosystems, Carlsbad, CA, USA).

Article Title: Overexpression of the Soybean NAC Gene GmNAC109 Increases Lateral Root Formation and Abiotic Stress Tolerance in Transgenic Arabidopsis Plants
Article Snippet: Paragraph title: Quantitative Real-Time PCR (qRT-PCR) ... Approximately 1 μg of total RNA was reverse-transcribed using an iScript™ cDNA Synthesis Kit (Cat. 1708891; Bio-Rad, CA, USA). qRT-PCR was performed using iQ™ SYBR® Green Supermix (Cat. 170-8880AP; Bio-Rad).

Article Title: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex
Article Snippet: Paragraph title: 2.4. RNA Extraction and Real-Time PCR Analysis ... The PCR reaction mixtures contained 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad, Cat. number 1708882AP), 0.5 μ M primers, and 0.8 μ L of cDNA.

Article Title: Impairment of Pseudomonas aeruginosa Biofilm Resistance to Antibiotics by Combining the Drugs with a New Quorum-Sensing Inhibitor
Article Snippet: .. The primer sequences were subjected to a BLAST analysis against the P. aeruginosa PAO1 genome sequence to eliminate the possibility of nonspecific binding. qPCR was carried out using the IQ SYBR green supermix (Bio-Rad, Marnes-la-Coquette, France) with a 25-μl reaction mixture containing 12.5 μl of 2× IQ SYBR green supermix, 0.2 μM each forward and reverse primer, and 5 μl of extracted DNA. qPCR was performed using an iCycler IQ5 real-time PCR detection system (Bio-Rad, Marnes-la-Coquette, France) and the following cycling parameters: an initial denaturation at 95°C for 3 min, followed by 50 cycles of 95°C for 10 s, 60°C for 30 s, and 72°C for 30 s. Depending on the melting temperature ( Tm ) of a specific amplicon, the melt curve identifies a characteristic Tm that can distinguish between amplicons differing by only a single base. .. For our study, a melt curve analysis was performed immediately after amplification at 95°C for 1 min and 55°C for 1 min, followed by 81 repeats of heating for 10 s starting at 55°C and increasing in 0.5°C increments.

Article Title: A novel, multiplex, real-time PCR-based approach for the detection of the commonly occurring pathogenic fungi and bacteria
Article Snippet: .. The tested dyes were LCGreen “LightCycler® 480 High Resolution Melting Master” (Roche Diagnostic GmbH, Mannheim, Germany); SybrGreen “LightCycler® 480 DNA Master SYBR Green I”, (Roche); “IQ™ SYBR® Green Supermix” (Bio-Rad Laboratries, Inc., Hercules, CA, USA); “Maxima™ SYBR Green qPCR Master Mix no ROX” (Fermentas, Vilnius, Lithuania); and EvaGreen (“LC-FastStart DNA Master Hybridization Probes” (Roche) combined with EvaGreen dye (Biotium Inc., Hayward, CA, USA) and “Sso Fast™ EvaGreen® Supermix” (BioRad). ..

Article Title: A functional microRNA screening method for organ morphogenesis
Article Snippet: 720001180) iQ™ SYBR® Green Supermix (BIORAD, cat.no. .. 218073) qPCR primers: Mature miRNA expression: miScript Primer Assays (QIAGEN) miRNA targets and other genes (Invitrogen primers)

RNA Extraction:

Article Title: High Expression of PTGR1 Promotes NSCLC Cell Growth via Positive Regulation of Cyclin-Dependent Protein Kinase Complex
Article Snippet: Paragraph title: 2.4. RNA Extraction and Real-Time PCR Analysis ... The PCR reaction mixtures contained 10 μ L of iQ™ SYBR® Green Supermix (Bio-Rad, Cat. number 1708882AP), 0.5 μ M primers, and 0.8 μ L of cDNA.

Concentration Assay:

Article Title: Rye (Secale cereale) supernumerary (B) chromosomes associated with heat tolerance during early stages of male sporogenesis
Article Snippet: After verifying concentration and integrity, 3 μg of total RNA was utilized for RNase-free DNase digestion. .. For transcript quantification, cDNA from leaves and staged anthers from 0B and 2B plants was analysed by qRT-PCR with the Bio-Rad IQ SYBR Green Supermix (cat. no. 170-8880S).

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    Bio-Rad iq sybr green supermix
    Iq Sybr Green Supermix, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 90/100, based on 3038 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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