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Transgenomic ip hplc system
Imprinting in mTECs ( Igf2 vs. Cdkn1c genes). Expression of Igf2 and Cdkn1c was analyzed by RT-PCR amplification and <t>SNuPE/HPLC</t> in mTECs and control tissues from the F 1 generation of C57BL/6 × SD7 and SD7 × C57BL/6 crosses. Elution profiles of the SNuPE products are shown. The first peak corresponds to unextended primers and the second and third peak to products transcribed from the maternal or paternal allele, respectively, as indicated. Igf2 is paternally expressed with the exception of the choroid plexus and leptomeninges. Note that biallelic expression also occurs in mTECs. In contrast, imprinting of Cdkn1c is maintained in all tissues tested including mTECs; i.e., the gene is maternally expressed. The analysis of genomic <t>DNA</t> (top right) indicates the position of both allele-specific PCR products. pat, paternal; mat, maternal.
Ip Hplc System, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 86 stars, based on 1 article reviews
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ip hplc system - by Bioz Stars, 2021-04
86/100 stars

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1) Product Images from "Promiscuous gene expression in thymic epithelial cells is regulated at multiple levels"

Article Title: Promiscuous gene expression in thymic epithelial cells is regulated at multiple levels

Journal: The Journal of Experimental Medicine

doi: 10.1084/jem.20050471

Imprinting in mTECs ( Igf2 vs. Cdkn1c genes). Expression of Igf2 and Cdkn1c was analyzed by RT-PCR amplification and SNuPE/HPLC in mTECs and control tissues from the F 1 generation of C57BL/6 × SD7 and SD7 × C57BL/6 crosses. Elution profiles of the SNuPE products are shown. The first peak corresponds to unextended primers and the second and third peak to products transcribed from the maternal or paternal allele, respectively, as indicated. Igf2 is paternally expressed with the exception of the choroid plexus and leptomeninges. Note that biallelic expression also occurs in mTECs. In contrast, imprinting of Cdkn1c is maintained in all tissues tested including mTECs; i.e., the gene is maternally expressed. The analysis of genomic DNA (top right) indicates the position of both allele-specific PCR products. pat, paternal; mat, maternal.
Figure Legend Snippet: Imprinting in mTECs ( Igf2 vs. Cdkn1c genes). Expression of Igf2 and Cdkn1c was analyzed by RT-PCR amplification and SNuPE/HPLC in mTECs and control tissues from the F 1 generation of C57BL/6 × SD7 and SD7 × C57BL/6 crosses. Elution profiles of the SNuPE products are shown. The first peak corresponds to unextended primers and the second and third peak to products transcribed from the maternal or paternal allele, respectively, as indicated. Igf2 is paternally expressed with the exception of the choroid plexus and leptomeninges. Note that biallelic expression also occurs in mTECs. In contrast, imprinting of Cdkn1c is maintained in all tissues tested including mTECs; i.e., the gene is maternally expressed. The analysis of genomic DNA (top right) indicates the position of both allele-specific PCR products. pat, paternal; mat, maternal.

Techniques Used: Expressing, Reverse Transcription Polymerase Chain Reaction, Amplification, High Performance Liquid Chromatography, Polymerase Chain Reaction

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Southern Blot:

Article Title: Lactic Acidosis in a Newborn With Adrenal Calcifications
Article Snippet: The antibodies used for immunostaining studies were NDUFS7 for complex I, IP subunit for complex II, core2 subunit for complex III, subunit I for complex IV, and the alpha-subunit for complex V (Molecular Probes, Eugene, OR). .. The search for pathologic alterations and mutations in skeletal muscle mitochondrial DNA (mtDNA) was performed using southern blot, PCR-single strand conformational polymorphism (PCR-SSCP), and denaturing HPLC (dHPLC, MitoScreen Assay Kit, Transgenomic). .. The quantitative mtDNA assessment was addressed by relative quantification of total mtDNA to nuclear DNA by real time PCR (ABI Prism 7500 Sequence Detection System, Applied Biosystems) in total cellular DNA isolated from heart muscle of the patient, as well as in similar tissue from age-matched control individuals ( ).

Polymerase Chain Reaction:

Article Title: Lactic Acidosis in a Newborn With Adrenal Calcifications
Article Snippet: The antibodies used for immunostaining studies were NDUFS7 for complex I, IP subunit for complex II, core2 subunit for complex III, subunit I for complex IV, and the alpha-subunit for complex V (Molecular Probes, Eugene, OR). .. The search for pathologic alterations and mutations in skeletal muscle mitochondrial DNA (mtDNA) was performed using southern blot, PCR-single strand conformational polymorphism (PCR-SSCP), and denaturing HPLC (dHPLC, MitoScreen Assay Kit, Transgenomic). .. The quantitative mtDNA assessment was addressed by relative quantification of total mtDNA to nuclear DNA by real time PCR (ABI Prism 7500 Sequence Detection System, Applied Biosystems) in total cellular DNA isolated from heart muscle of the patient, as well as in similar tissue from age-matched control individuals ( ).

High Performance Liquid Chromatography:

Article Title: Lactic Acidosis in a Newborn With Adrenal Calcifications
Article Snippet: The antibodies used for immunostaining studies were NDUFS7 for complex I, IP subunit for complex II, core2 subunit for complex III, subunit I for complex IV, and the alpha-subunit for complex V (Molecular Probes, Eugene, OR). .. The search for pathologic alterations and mutations in skeletal muscle mitochondrial DNA (mtDNA) was performed using southern blot, PCR-single strand conformational polymorphism (PCR-SSCP), and denaturing HPLC (dHPLC, MitoScreen Assay Kit, Transgenomic). .. The quantitative mtDNA assessment was addressed by relative quantification of total mtDNA to nuclear DNA by real time PCR (ABI Prism 7500 Sequence Detection System, Applied Biosystems) in total cellular DNA isolated from heart muscle of the patient, as well as in similar tissue from age-matched control individuals ( ).

Article Title: Genome-Wide Association Study to Identify Genes Related to Renal Mercury Concentrations in Mice
Article Snippet: .. Ion Pair Reverse-Phase High-Performance Liquid Chromatography Microsatellites between 2 and 10 bp were detected using ion pair reverse-phase high-performance liquid chromatography (IP RP HPLC) on a Transgenomic WAVE system (Transgenomic). .. The mobile phase consisted of 0.1 M triethylammonium acetate (TEAA; Applied Biosystems) (Solvent A) and 0.1 M TEAA–25% acetonitrile (ACN; EM Science) (Solvent B).

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    Transgenomic ip rp hplc
    IP RP <t>HPLC</t> analyses of dsRNA and ssRNA under denaturing and non-denaturing conditions. (a) IP RP HPLC chromatogram of dsRNA at 50 °C. (b) IP RP HPLC chromatogram of dsRNA at 75 °C. (c) IP RP HPLC chromatogram of ssRNA at 50 °C. (d) IP RP HPLC chromatogram of ssRNA at 75 °C. (e) IP RP HPLC chromatogram of in vitro transcribed dsRNA with an excess of ssRNA. (f) IP RP HPLC chromatogram of purified dsRNA. Following in vitro transcription, the <t>RNA</t> was purified in a single step using RNase T1/DNase in conjunction with solid phase extraction. 2–3 μg of in vitro transcribed ds/ssRNA was analysed using gradient 1 at 260 nm UV detection.
    Ip Rp Hplc, supplied by Transgenomic, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ip rp hplc/product/Transgenomic
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ip rp hplc - by Bioz Stars, 2021-04
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    IP RP HPLC analyses of dsRNA and ssRNA under denaturing and non-denaturing conditions. (a) IP RP HPLC chromatogram of dsRNA at 50 °C. (b) IP RP HPLC chromatogram of dsRNA at 75 °C. (c) IP RP HPLC chromatogram of ssRNA at 50 °C. (d) IP RP HPLC chromatogram of ssRNA at 75 °C. (e) IP RP HPLC chromatogram of in vitro transcribed dsRNA with an excess of ssRNA. (f) IP RP HPLC chromatogram of purified dsRNA. Following in vitro transcription, the RNA was purified in a single step using RNase T1/DNase in conjunction with solid phase extraction. 2–3 μg of in vitro transcribed ds/ssRNA was analysed using gradient 1 at 260 nm UV detection.

    Journal: Journal of Chromatography. a

    Article Title: Purification and characterisation of dsRNA using ion pair reverse phase chromatography and mass spectrometry

    doi: 10.1016/j.chroma.2016.12.062

    Figure Lengend Snippet: IP RP HPLC analyses of dsRNA and ssRNA under denaturing and non-denaturing conditions. (a) IP RP HPLC chromatogram of dsRNA at 50 °C. (b) IP RP HPLC chromatogram of dsRNA at 75 °C. (c) IP RP HPLC chromatogram of ssRNA at 50 °C. (d) IP RP HPLC chromatogram of ssRNA at 75 °C. (e) IP RP HPLC chromatogram of in vitro transcribed dsRNA with an excess of ssRNA. (f) IP RP HPLC chromatogram of purified dsRNA. Following in vitro transcription, the RNA was purified in a single step using RNase T1/DNase in conjunction with solid phase extraction. 2–3 μg of in vitro transcribed ds/ssRNA was analysed using gradient 1 at 260 nm UV detection.

    Article Snippet: RNA extracted from the E. coli cells using TRIzol® (TRIzol® Max™ Bacterial RNA Isolation Kit) and analysed using IP RP HPLC is shown in .

    Techniques: High Performance Liquid Chromatography, In Vitro, Purification

    IP RP HPLC chromatograms of total RNA extracted from E. coli using TRIzol ® Max™ Bacterial RNA Isolation Kit. (a) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV prior to induction. (b) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV following induction with IPTG. The corresponding rRNA and dsRNA are highlighted. Approximately 15 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection. (c) IP RP HPLC chromatogram of total RNA extracted from E. coli HT115 (DE3) in conjunction with mechanical shearing and TRIzol. Approximately 10 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection.

    Journal: Journal of Chromatography. a

    Article Title: Purification and characterisation of dsRNA using ion pair reverse phase chromatography and mass spectrometry

    doi: 10.1016/j.chroma.2016.12.062

    Figure Lengend Snippet: IP RP HPLC chromatograms of total RNA extracted from E. coli using TRIzol ® Max™ Bacterial RNA Isolation Kit. (a) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV prior to induction. (b) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV following induction with IPTG. The corresponding rRNA and dsRNA are highlighted. Approximately 15 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection. (c) IP RP HPLC chromatogram of total RNA extracted from E. coli HT115 (DE3) in conjunction with mechanical shearing and TRIzol. Approximately 10 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection.

    Article Snippet: RNA extracted from the E. coli cells using TRIzol® (TRIzol® Max™ Bacterial RNA Isolation Kit) and analysed using IP RP HPLC is shown in .

    Techniques: High Performance Liquid Chromatography, Isolation, Transformation Assay, Plasmid Preparation

    Purification of dsRNA from E. coli . (a) Agarose gel electrophoresis of purified in vitro transcribed dsRNA and ssRNA. Following in vitro transcription, 2 μg of dsRNA containing excess ssRNA were incubated with RNase T1 in both the presence and absence of 0.5 M NaCl and purified by SPE. (b) Agarose gel electrophoresis of extracted and purified dsRNA. Following TRIzol extraction of total RNA from E. coli expressing dsRNA samples were incubated with RNase T1 in both the presence and absence of 0.3 M NaCl as indicated prior to purification using solid phase extraction. Control experiments were performed using total RNA from non-induced E. coli . (c) IP RP HPLC chromatogram of purified dsRNA. Following TRIzol extraction the total RNA from E. coli was purified in a single step using RNase T1/DNase in conjunction with solid phase extraction prior to analysis using IP RP HPLC gradient condition 1 at 260 nm UV detection.

    Journal: Journal of Chromatography. a

    Article Title: Purification and characterisation of dsRNA using ion pair reverse phase chromatography and mass spectrometry

    doi: 10.1016/j.chroma.2016.12.062

    Figure Lengend Snippet: Purification of dsRNA from E. coli . (a) Agarose gel electrophoresis of purified in vitro transcribed dsRNA and ssRNA. Following in vitro transcription, 2 μg of dsRNA containing excess ssRNA were incubated with RNase T1 in both the presence and absence of 0.5 M NaCl and purified by SPE. (b) Agarose gel electrophoresis of extracted and purified dsRNA. Following TRIzol extraction of total RNA from E. coli expressing dsRNA samples were incubated with RNase T1 in both the presence and absence of 0.3 M NaCl as indicated prior to purification using solid phase extraction. Control experiments were performed using total RNA from non-induced E. coli . (c) IP RP HPLC chromatogram of purified dsRNA. Following TRIzol extraction the total RNA from E. coli was purified in a single step using RNase T1/DNase in conjunction with solid phase extraction prior to analysis using IP RP HPLC gradient condition 1 at 260 nm UV detection.

    Article Snippet: RNA extracted from the E. coli cells using TRIzol® (TRIzol® Max™ Bacterial RNA Isolation Kit) and analysed using IP RP HPLC is shown in .

    Techniques: Purification, Agarose Gel Electrophoresis, In Vitro, Incubation, Expressing, High Performance Liquid Chromatography

    IP RP HPLC chromatograms of total RNA extracted from E. coli using Ribopure™ bacterial RNA extraction kit. (a) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV prior to induction. (b) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV following induction with IPTG. The corresponding 5S, 16S and 23S rRNA are highlighted. 2 and 8 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection.

    Journal: Journal of Chromatography. a

    Article Title: Purification and characterisation of dsRNA using ion pair reverse phase chromatography and mass spectrometry

    doi: 10.1016/j.chroma.2016.12.062

    Figure Lengend Snippet: IP RP HPLC chromatograms of total RNA extracted from E. coli using Ribopure™ bacterial RNA extraction kit. (a) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV prior to induction. (b) IP RP HPLC chromatogram of total RNA from E. coli HT115 (DE3) cells transformed with plasmid pCOIV following induction with IPTG. The corresponding 5S, 16S and 23S rRNA are highlighted. 2 and 8 μg of total RNA was analysed using gradient condition 1 at 260 nm UV detection.

    Article Snippet: RNA extracted from the E. coli cells using TRIzol® (TRIzol® Max™ Bacterial RNA Isolation Kit) and analysed using IP RP HPLC is shown in .

    Techniques: High Performance Liquid Chromatography, RNA Extraction, Transformation Assay, Plasmid Preparation