ionomycin  (LKT Laboratories)

 
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    Name:
    Ionomycin Calcium
    Description:
    Polyether Ca2 ionophore
    Catalog Number:
    i5753
    Price:
    35.7
    Purity:
    ≥98%
    Size:
    1 mg
    Category:
    Microbiology
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    Structured Review

    LKT Laboratories ionomycin
    TAT-CBD3 inhibits the forward mode of NCX. Neurons were loaded with Fura-2FF. A , 5 μ m <t>ionomycin</t> in combination with 20 μ m AP-5 was applied to neurons. Where indicated, neurons were subjected to Na + /NMDG replacement ( B ) or treated with
    Polyether Ca2 ionophore
    https://www.bioz.com/result/ionomycin/product/LKT Laboratories
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ionomycin - by Bioz Stars, 2020-09
    94/100 stars

    Images

    1) Product Images from "Collapsin Response Mediator Protein 2 (CRMP2) Interacts with N-Methyl-d-aspartate (NMDA) Receptor and Na+/Ca2+ Exchanger and Regulates Their Functional Activity *"

    Article Title: Collapsin Response Mediator Protein 2 (CRMP2) Interacts with N-Methyl-d-aspartate (NMDA) Receptor and Na+/Ca2+ Exchanger and Regulates Their Functional Activity *

    Journal: The Journal of Biological Chemistry

    doi: 10.1074/jbc.M113.518472

    TAT-CBD3 inhibits the forward mode of NCX. Neurons were loaded with Fura-2FF. A , 5 μ m ionomycin in combination with 20 μ m AP-5 was applied to neurons. Where indicated, neurons were subjected to Na + /NMDG replacement ( B ) or treated with
    Figure Legend Snippet: TAT-CBD3 inhibits the forward mode of NCX. Neurons were loaded with Fura-2FF. A , 5 μ m ionomycin in combination with 20 μ m AP-5 was applied to neurons. Where indicated, neurons were subjected to Na + /NMDG replacement ( B ) or treated with

    Techniques Used:

    2) Product Images from "The novel cyclophilin-D-interacting protein FASTKD1 protects cells against oxidative stress-induced cell death"

    Article Title: The novel cyclophilin-D-interacting protein FASTKD1 protects cells against oxidative stress-induced cell death

    Journal: American Journal of Physiology - Cell Physiology

    doi: 10.1152/ajpcell.00471.2018

    FASTKD1 protects fibroblasts against oxidative stress-induced cell death. A : cell death as measured by Sytox staining in β-galactosidase (βGal)- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of H 2 O 2 for 4 h. B : cell death as measured by Sytox staining in βGal- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of β-Lapachone for 4 h. C : NRVMs were transfected with 100 nM control siRNA (CONsi) or mouse-specific FASTKD1 siRNA (FASTKD1si) followed by infection with βGal or FASTKD1-Myc adenoviruses. The lysates were then blotted for Myc. GAPDH was used as a loading control. D : cell death as measured by Sytox staining in CONsi- or FASTKD1si-transfected MEFs treated with increasing concentrations of H 2 O 2 for 4 h. E : cell death as measured by Sytox staining in βGal- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of ionomycin for 18 h. F : cell death as measured by Sytox staining in CONsi- or FASTKD1si siRNA-transfected MEFs treated with increasing concentrations of ionomycin for 4 h. Each individual point represents one independent cell isolate. Bar represents the mean. Cells of both sexes were used. Differences in cell death between groups were analyzed using 2-way ANOVA followed by Scheffé’s post hoc test. * P
    Figure Legend Snippet: FASTKD1 protects fibroblasts against oxidative stress-induced cell death. A : cell death as measured by Sytox staining in β-galactosidase (βGal)- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of H 2 O 2 for 4 h. B : cell death as measured by Sytox staining in βGal- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of β-Lapachone for 4 h. C : NRVMs were transfected with 100 nM control siRNA (CONsi) or mouse-specific FASTKD1 siRNA (FASTKD1si) followed by infection with βGal or FASTKD1-Myc adenoviruses. The lysates were then blotted for Myc. GAPDH was used as a loading control. D : cell death as measured by Sytox staining in CONsi- or FASTKD1si-transfected MEFs treated with increasing concentrations of H 2 O 2 for 4 h. E : cell death as measured by Sytox staining in βGal- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of ionomycin for 18 h. F : cell death as measured by Sytox staining in CONsi- or FASTKD1si siRNA-transfected MEFs treated with increasing concentrations of ionomycin for 4 h. Each individual point represents one independent cell isolate. Bar represents the mean. Cells of both sexes were used. Differences in cell death between groups were analyzed using 2-way ANOVA followed by Scheffé’s post hoc test. * P

    Techniques Used: Staining, Infection, Transfection

    3) Product Images from "Isoflavones enhance interleukin-17 gene expression via retinoic acid receptor-related orphan receptors α and γ"

    Article Title: Isoflavones enhance interleukin-17 gene expression via retinoic acid receptor-related orphan receptors α and γ

    Journal: Toxicology

    doi: 10.1016/j.tox.2015.01.007

    Comparison of enhancement of Il17a mRNA expression by biochanin A using EL4/shRorc cells and EL4/shNC cells. Il17a mRNA expression was measured in EL4/shRorac cells and EL4/shNC cells in the presence or absence of biochanin A (1 × 10 −5 M) with PMA/ionomycin stimulation, and normalized against the expression of the β-actin housekeeping gene. Values are expressed relative to the vehicle control with PMA/ionomycin stimulation in EL4/shNC cells (taken as 1) and represent the mean ± SD of three experiments. Significant differences from the vehicle control with PMA/ionomycine stimulation are indicated by asterisks (* P
    Figure Legend Snippet: Comparison of enhancement of Il17a mRNA expression by biochanin A using EL4/shRorc cells and EL4/shNC cells. Il17a mRNA expression was measured in EL4/shRorac cells and EL4/shNC cells in the presence or absence of biochanin A (1 × 10 −5 M) with PMA/ionomycin stimulation, and normalized against the expression of the β-actin housekeeping gene. Values are expressed relative to the vehicle control with PMA/ionomycin stimulation in EL4/shNC cells (taken as 1) and represent the mean ± SD of three experiments. Significant differences from the vehicle control with PMA/ionomycine stimulation are indicated by asterisks (* P

    Techniques Used: Expressing

    Enhancement of Il17a mRNA expression by isoflavones in EL4 cells treated with PMA and ionomycin. EL4 cells were treated with PMA (5 ng/ml) and ionomycin (1 μM) for Il17a gene induction. At the same time, the cells were treated with the vehicle, or 0.1, 1 or 10 μM of the isoflavones, and incubated at 37 °C for 6 h. Il17a mRNA expression in the cells was amplified by real-time RT-PCR and normalized against the expression of the β-actin housekeeping gene. BA, GE, FN, and DZ represent each isoflavone, biochanin A, genistein, formononetin, and daidzein, respectively. Values are expressed relative to the vehicle control with PMA/ionomycin stimulation (taken as 1) and represent the mean ± SD of three experiments. Significant differences from the vehicle control with PMA/ionomycine stimulation are indicated by asterisks (* P
    Figure Legend Snippet: Enhancement of Il17a mRNA expression by isoflavones in EL4 cells treated with PMA and ionomycin. EL4 cells were treated with PMA (5 ng/ml) and ionomycin (1 μM) for Il17a gene induction. At the same time, the cells were treated with the vehicle, or 0.1, 1 or 10 μM of the isoflavones, and incubated at 37 °C for 6 h. Il17a mRNA expression in the cells was amplified by real-time RT-PCR and normalized against the expression of the β-actin housekeeping gene. BA, GE, FN, and DZ represent each isoflavone, biochanin A, genistein, formononetin, and daidzein, respectively. Values are expressed relative to the vehicle control with PMA/ionomycin stimulation (taken as 1) and represent the mean ± SD of three experiments. Significant differences from the vehicle control with PMA/ionomycine stimulation are indicated by asterisks (* P

    Techniques Used: Expressing, Incubation, Amplification, Quantitative RT-PCR

    4) Product Images from "Nod2-deficiency augments Th17-responses and exacerbates autoimmune arthritis"

    Article Title: Nod2-deficiency augments Th17-responses and exacerbates autoimmune arthritis

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    doi: 10.4049/jimmunol.1700507

    Nod2-deficiency results in increased T cellular responses and augmented Th17 immunity in arthritic SKG mice. The T cellular response was evaluated 8 wk following injection of zymosan into SKG or Nod2 −/− SKG mice. (A) Combined weight of popliteal lymph nodes (2/mouse). (B) Quantification of the total number of live T cell subsets from the dLN by flow cytometry. (C) The number of live T cell subsets from synovial fluid aspirated from the sub-capsular space of ankle joints was quantified by flow cytometry. (D-G) Single cell suspensions from combined popliteal lymph nodes were stimulated in vitro with PMA/Ionomycin (PMA/Io), stained for intracellular cytokines, then analyzed by flow cytometry. (D) Dot plots showing frequency of IL-17-producing CD4 + T cells. Th17 cells were quantified and are shown as (E) percentage and (F) total number of live CD4 + T cells. (G) The Th17 cell population was gated and the frequency of Th17 cells co-expressing cytokines (IFNγ, TNF, GM-CSF, and IL-22) was quantified. (H) IL-17A protein in synovial fluid from the sub-capsular space of ankle joints 8 wk post-zymosan was quantified by ELISA. Data are graphed as median with interquartile range. All data (expect panel H) are graphed as box and whisker plots demarcating the median with min to max whiskers and are combined from 2 independently performed studies. * p
    Figure Legend Snippet: Nod2-deficiency results in increased T cellular responses and augmented Th17 immunity in arthritic SKG mice. The T cellular response was evaluated 8 wk following injection of zymosan into SKG or Nod2 −/− SKG mice. (A) Combined weight of popliteal lymph nodes (2/mouse). (B) Quantification of the total number of live T cell subsets from the dLN by flow cytometry. (C) The number of live T cell subsets from synovial fluid aspirated from the sub-capsular space of ankle joints was quantified by flow cytometry. (D-G) Single cell suspensions from combined popliteal lymph nodes were stimulated in vitro with PMA/Ionomycin (PMA/Io), stained for intracellular cytokines, then analyzed by flow cytometry. (D) Dot plots showing frequency of IL-17-producing CD4 + T cells. Th17 cells were quantified and are shown as (E) percentage and (F) total number of live CD4 + T cells. (G) The Th17 cell population was gated and the frequency of Th17 cells co-expressing cytokines (IFNγ, TNF, GM-CSF, and IL-22) was quantified. (H) IL-17A protein in synovial fluid from the sub-capsular space of ankle joints 8 wk post-zymosan was quantified by ELISA. Data are graphed as median with interquartile range. All data (expect panel H) are graphed as box and whisker plots demarcating the median with min to max whiskers and are combined from 2 independently performed studies. * p

    Techniques Used: Mouse Assay, Injection, Flow Cytometry, Cytometry, In Vitro, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Whisker Assay

    5) Product Images from "Inhibitory effects of azole-type fungicides on interleukin-17 gene expression via retinoic acid receptor-related orphan receptors ? and ?"

    Article Title: Inhibitory effects of azole-type fungicides on interleukin-17 gene expression via retinoic acid receptor-related orphan receptors ? and ?

    Journal: Toxicology and applied pharmacology

    doi: 10.1016/j.taap.2012.01.011

    Suppression of IL-17 mRNA expression by azole-type fungicides in EL4 cells treated with PMA and ionomycin
    Figure Legend Snippet: Suppression of IL-17 mRNA expression by azole-type fungicides in EL4 cells treated with PMA and ionomycin

    Techniques Used: Expressing

    Related Articles

    other:

    Article Title: Blockade of L-type Ca2+ channel attenuates doxorubicin-induced cardiomyopathy via suppression of CaMKII-NF-κB pathway
    Article Snippet: The resting levels of intracellular Ca2+ levels were expressed as percentages by assigning the levels of intracellular Ca2+ obtained with ionomycin, (a calcium ionophore, LKT Laboratories, St. Paul, MN) in the presence and absence of intracellular and extracellular Ca2+ , to be 100% and 0%, respectively.

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  • 94
    LKT Laboratories ionomycin
    TAT-CBD3 inhibits the forward mode of NCX. Neurons were loaded with Fura-2FF. A , 5 μ m <t>ionomycin</t> in combination with 20 μ m AP-5 was applied to neurons. Where indicated, neurons were subjected to Na + /NMDG replacement ( B ) or treated with
    Ionomycin, supplied by LKT Laboratories, used in various techniques. Bioz Stars score: 94/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ionomycin/product/LKT Laboratories
    Average 94 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    ionomycin - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

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    TAT-CBD3 inhibits the forward mode of NCX. Neurons were loaded with Fura-2FF. A , 5 μ m ionomycin in combination with 20 μ m AP-5 was applied to neurons. Where indicated, neurons were subjected to Na + /NMDG replacement ( B ) or treated with

    Journal: The Journal of Biological Chemistry

    Article Title: Collapsin Response Mediator Protein 2 (CRMP2) Interacts with N-Methyl-d-aspartate (NMDA) Receptor and Na+/Ca2+ Exchanger and Regulates Their Functional Activity *

    doi: 10.1074/jbc.M113.518472

    Figure Lengend Snippet: TAT-CBD3 inhibits the forward mode of NCX. Neurons were loaded with Fura-2FF. A , 5 μ m ionomycin in combination with 20 μ m AP-5 was applied to neurons. Where indicated, neurons were subjected to Na + /NMDG replacement ( B ) or treated with

    Article Snippet: Ionomycin was from LKT Laboratories (St. Paul, MN).

    Techniques:

    FASTKD1 protects fibroblasts against oxidative stress-induced cell death. A : cell death as measured by Sytox staining in β-galactosidase (βGal)- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of H 2 O 2 for 4 h. B : cell death as measured by Sytox staining in βGal- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of β-Lapachone for 4 h. C : NRVMs were transfected with 100 nM control siRNA (CONsi) or mouse-specific FASTKD1 siRNA (FASTKD1si) followed by infection with βGal or FASTKD1-Myc adenoviruses. The lysates were then blotted for Myc. GAPDH was used as a loading control. D : cell death as measured by Sytox staining in CONsi- or FASTKD1si-transfected MEFs treated with increasing concentrations of H 2 O 2 for 4 h. E : cell death as measured by Sytox staining in βGal- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of ionomycin for 18 h. F : cell death as measured by Sytox staining in CONsi- or FASTKD1si siRNA-transfected MEFs treated with increasing concentrations of ionomycin for 4 h. Each individual point represents one independent cell isolate. Bar represents the mean. Cells of both sexes were used. Differences in cell death between groups were analyzed using 2-way ANOVA followed by Scheffé’s post hoc test. * P

    Journal: American Journal of Physiology - Cell Physiology

    Article Title: The novel cyclophilin-D-interacting protein FASTKD1 protects cells against oxidative stress-induced cell death

    doi: 10.1152/ajpcell.00471.2018

    Figure Lengend Snippet: FASTKD1 protects fibroblasts against oxidative stress-induced cell death. A : cell death as measured by Sytox staining in β-galactosidase (βGal)- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of H 2 O 2 for 4 h. B : cell death as measured by Sytox staining in βGal- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of β-Lapachone for 4 h. C : NRVMs were transfected with 100 nM control siRNA (CONsi) or mouse-specific FASTKD1 siRNA (FASTKD1si) followed by infection with βGal or FASTKD1-Myc adenoviruses. The lysates were then blotted for Myc. GAPDH was used as a loading control. D : cell death as measured by Sytox staining in CONsi- or FASTKD1si-transfected MEFs treated with increasing concentrations of H 2 O 2 for 4 h. E : cell death as measured by Sytox staining in βGal- or FASTKD1-Myc-infected MEFs treated with increasing concentrations of ionomycin for 18 h. F : cell death as measured by Sytox staining in CONsi- or FASTKD1si siRNA-transfected MEFs treated with increasing concentrations of ionomycin for 4 h. Each individual point represents one independent cell isolate. Bar represents the mean. Cells of both sexes were used. Differences in cell death between groups were analyzed using 2-way ANOVA followed by Scheffé’s post hoc test. * P

    Article Snippet: MEFs were treated with β-lapachone (Axxora) or ionomycin (LKT Laboratories) for 4 h or 18 h, respectively, at the indicated concentrations.

    Techniques: Staining, Infection, Transfection

    Comparison of enhancement of Il17a mRNA expression by biochanin A using EL4/shRorc cells and EL4/shNC cells. Il17a mRNA expression was measured in EL4/shRorac cells and EL4/shNC cells in the presence or absence of biochanin A (1 × 10 −5 M) with PMA/ionomycin stimulation, and normalized against the expression of the β-actin housekeeping gene. Values are expressed relative to the vehicle control with PMA/ionomycin stimulation in EL4/shNC cells (taken as 1) and represent the mean ± SD of three experiments. Significant differences from the vehicle control with PMA/ionomycine stimulation are indicated by asterisks (* P

    Journal: Toxicology

    Article Title: Isoflavones enhance interleukin-17 gene expression via retinoic acid receptor-related orphan receptors α and γ

    doi: 10.1016/j.tox.2015.01.007

    Figure Lengend Snippet: Comparison of enhancement of Il17a mRNA expression by biochanin A using EL4/shRorc cells and EL4/shNC cells. Il17a mRNA expression was measured in EL4/shRorac cells and EL4/shNC cells in the presence or absence of biochanin A (1 × 10 −5 M) with PMA/ionomycin stimulation, and normalized against the expression of the β-actin housekeeping gene. Values are expressed relative to the vehicle control with PMA/ionomycin stimulation in EL4/shNC cells (taken as 1) and represent the mean ± SD of three experiments. Significant differences from the vehicle control with PMA/ionomycine stimulation are indicated by asterisks (* P

    Article Snippet: Ionomycin was purchased from LKT Laboratories (St. Paul, MN, USA).

    Techniques: Expressing

    Enhancement of Il17a mRNA expression by isoflavones in EL4 cells treated with PMA and ionomycin. EL4 cells were treated with PMA (5 ng/ml) and ionomycin (1 μM) for Il17a gene induction. At the same time, the cells were treated with the vehicle, or 0.1, 1 or 10 μM of the isoflavones, and incubated at 37 °C for 6 h. Il17a mRNA expression in the cells was amplified by real-time RT-PCR and normalized against the expression of the β-actin housekeeping gene. BA, GE, FN, and DZ represent each isoflavone, biochanin A, genistein, formononetin, and daidzein, respectively. Values are expressed relative to the vehicle control with PMA/ionomycin stimulation (taken as 1) and represent the mean ± SD of three experiments. Significant differences from the vehicle control with PMA/ionomycine stimulation are indicated by asterisks (* P

    Journal: Toxicology

    Article Title: Isoflavones enhance interleukin-17 gene expression via retinoic acid receptor-related orphan receptors α and γ

    doi: 10.1016/j.tox.2015.01.007

    Figure Lengend Snippet: Enhancement of Il17a mRNA expression by isoflavones in EL4 cells treated with PMA and ionomycin. EL4 cells were treated with PMA (5 ng/ml) and ionomycin (1 μM) for Il17a gene induction. At the same time, the cells were treated with the vehicle, or 0.1, 1 or 10 μM of the isoflavones, and incubated at 37 °C for 6 h. Il17a mRNA expression in the cells was amplified by real-time RT-PCR and normalized against the expression of the β-actin housekeeping gene. BA, GE, FN, and DZ represent each isoflavone, biochanin A, genistein, formononetin, and daidzein, respectively. Values are expressed relative to the vehicle control with PMA/ionomycin stimulation (taken as 1) and represent the mean ± SD of three experiments. Significant differences from the vehicle control with PMA/ionomycine stimulation are indicated by asterisks (* P

    Article Snippet: Ionomycin was purchased from LKT Laboratories (St. Paul, MN, USA).

    Techniques: Expressing, Incubation, Amplification, Quantitative RT-PCR

    Nod2-deficiency results in increased T cellular responses and augmented Th17 immunity in arthritic SKG mice. The T cellular response was evaluated 8 wk following injection of zymosan into SKG or Nod2 −/− SKG mice. (A) Combined weight of popliteal lymph nodes (2/mouse). (B) Quantification of the total number of live T cell subsets from the dLN by flow cytometry. (C) The number of live T cell subsets from synovial fluid aspirated from the sub-capsular space of ankle joints was quantified by flow cytometry. (D-G) Single cell suspensions from combined popliteal lymph nodes were stimulated in vitro with PMA/Ionomycin (PMA/Io), stained for intracellular cytokines, then analyzed by flow cytometry. (D) Dot plots showing frequency of IL-17-producing CD4 + T cells. Th17 cells were quantified and are shown as (E) percentage and (F) total number of live CD4 + T cells. (G) The Th17 cell population was gated and the frequency of Th17 cells co-expressing cytokines (IFNγ, TNF, GM-CSF, and IL-22) was quantified. (H) IL-17A protein in synovial fluid from the sub-capsular space of ankle joints 8 wk post-zymosan was quantified by ELISA. Data are graphed as median with interquartile range. All data (expect panel H) are graphed as box and whisker plots demarcating the median with min to max whiskers and are combined from 2 independently performed studies. * p

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Nod2-deficiency augments Th17-responses and exacerbates autoimmune arthritis

    doi: 10.4049/jimmunol.1700507

    Figure Lengend Snippet: Nod2-deficiency results in increased T cellular responses and augmented Th17 immunity in arthritic SKG mice. The T cellular response was evaluated 8 wk following injection of zymosan into SKG or Nod2 −/− SKG mice. (A) Combined weight of popliteal lymph nodes (2/mouse). (B) Quantification of the total number of live T cell subsets from the dLN by flow cytometry. (C) The number of live T cell subsets from synovial fluid aspirated from the sub-capsular space of ankle joints was quantified by flow cytometry. (D-G) Single cell suspensions from combined popliteal lymph nodes were stimulated in vitro with PMA/Ionomycin (PMA/Io), stained for intracellular cytokines, then analyzed by flow cytometry. (D) Dot plots showing frequency of IL-17-producing CD4 + T cells. Th17 cells were quantified and are shown as (E) percentage and (F) total number of live CD4 + T cells. (G) The Th17 cell population was gated and the frequency of Th17 cells co-expressing cytokines (IFNγ, TNF, GM-CSF, and IL-22) was quantified. (H) IL-17A protein in synovial fluid from the sub-capsular space of ankle joints 8 wk post-zymosan was quantified by ELISA. Data are graphed as median with interquartile range. All data (expect panel H) are graphed as box and whisker plots demarcating the median with min to max whiskers and are combined from 2 independently performed studies. * p

    Article Snippet: Cells were treated with 20 ng/ml PMA (LC Laboratories) and 1 ¼g/ml ionomycin (LKT Laboratories) vs. media for 5 h in the presence of Brefeldin A (1 ¼g/ml; BD Biosciences).

    Techniques: Mouse Assay, Injection, Flow Cytometry, Cytometry, In Vitro, Staining, Expressing, Enzyme-linked Immunosorbent Assay, Whisker Assay