ionomycin  (InvivoGen)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    Name:
    Ionomycin
    Description:
    Calcium ionophore NFAT Activator
    Catalog Number:
    inh-ion
    Price:
    None
    Size:
    1 mg
    Category:
    Ionomycin NF κB NFAT Activators PRR Ligands
    Buy from Supplier


    Structured Review

    InvivoGen ionomycin
    T cell development and stress-induced apoptosis are normal in A1 −/− mice. ( a , left) Representative FACS plots of developing T cells in the thymus of wild-type and A1 −/− mice. T cells develop in the thymus from the CD4/CD8 double-negative (DN) stage, becoming double positive (DP) and then differentiating into either CD4 or CD8 single-positive cells. The DN population is further subdivided into DN1–4 phases: DN1 (CD44 + CD25 − ), DN2 (CD44 + CD25 + ), DN3 (CD44 − CD25 + ) and DN4 (CD44 − CD25 − ). ( a , right) Quantified total cell numbers for developing T cell populations in wild-type and A1 −/− mice. ( b ) Thymocytes harvested from wild-type and A1 −/− mice were put into culture with the following apoptosis-inducing compounds: ABT-737 (737) 0.5 μ M, dexamethasone (Dexa) 0.5 ng/ml, etoposide (Etopo) 0.5 μ g/ml, <t>ionomycin</t> (Iono), 0.1 μ g/ml, PMA 0.5 ng/ml or DMSO as a control. Apoptosis was assessed by FACS analysis after staining with fluorochrome-conjugated Annexin-V and PI (5 μ g/ml) at 24 h post treatment. Data are representative of six mice for each genotype, with each treatment performed in triplicate
    Calcium ionophore NFAT Activator
    https://www.bioz.com/result/ionomycin/product/InvivoGen
    Average 96 stars, based on 11 article reviews
    Price from $9.99 to $1999.99
    ionomycin - by Bioz Stars, 2020-09
    96/100 stars

    Images

    1) Product Images from "Characterisation of mice lacking all functional isoforms of the pro-survival BCL-2 family member A1 reveals minor defects in the haematopoietic compartment"

    Article Title: Characterisation of mice lacking all functional isoforms of the pro-survival BCL-2 family member A1 reveals minor defects in the haematopoietic compartment

    Journal: Cell Death and Differentiation

    doi: 10.1038/cdd.2016.156

    T cell development and stress-induced apoptosis are normal in A1 −/− mice. ( a , left) Representative FACS plots of developing T cells in the thymus of wild-type and A1 −/− mice. T cells develop in the thymus from the CD4/CD8 double-negative (DN) stage, becoming double positive (DP) and then differentiating into either CD4 or CD8 single-positive cells. The DN population is further subdivided into DN1–4 phases: DN1 (CD44 + CD25 − ), DN2 (CD44 + CD25 + ), DN3 (CD44 − CD25 + ) and DN4 (CD44 − CD25 − ). ( a , right) Quantified total cell numbers for developing T cell populations in wild-type and A1 −/− mice. ( b ) Thymocytes harvested from wild-type and A1 −/− mice were put into culture with the following apoptosis-inducing compounds: ABT-737 (737) 0.5 μ M, dexamethasone (Dexa) 0.5 ng/ml, etoposide (Etopo) 0.5 μ g/ml, ionomycin (Iono), 0.1 μ g/ml, PMA 0.5 ng/ml or DMSO as a control. Apoptosis was assessed by FACS analysis after staining with fluorochrome-conjugated Annexin-V and PI (5 μ g/ml) at 24 h post treatment. Data are representative of six mice for each genotype, with each treatment performed in triplicate
    Figure Legend Snippet: T cell development and stress-induced apoptosis are normal in A1 −/− mice. ( a , left) Representative FACS plots of developing T cells in the thymus of wild-type and A1 −/− mice. T cells develop in the thymus from the CD4/CD8 double-negative (DN) stage, becoming double positive (DP) and then differentiating into either CD4 or CD8 single-positive cells. The DN population is further subdivided into DN1–4 phases: DN1 (CD44 + CD25 − ), DN2 (CD44 + CD25 + ), DN3 (CD44 − CD25 + ) and DN4 (CD44 − CD25 − ). ( a , right) Quantified total cell numbers for developing T cell populations in wild-type and A1 −/− mice. ( b ) Thymocytes harvested from wild-type and A1 −/− mice were put into culture with the following apoptosis-inducing compounds: ABT-737 (737) 0.5 μ M, dexamethasone (Dexa) 0.5 ng/ml, etoposide (Etopo) 0.5 μ g/ml, ionomycin (Iono), 0.1 μ g/ml, PMA 0.5 ng/ml or DMSO as a control. Apoptosis was assessed by FACS analysis after staining with fluorochrome-conjugated Annexin-V and PI (5 μ g/ml) at 24 h post treatment. Data are representative of six mice for each genotype, with each treatment performed in triplicate

    Techniques Used: Mouse Assay, FACS, Staining

    2) Product Images from "Schistosomes Induce Regulatory Features in Human and Mouse CD1dhi B Cells: Inhibition of Allergic Inflammation by IL-10 and Regulatory T Cells"

    Article Title: Schistosomes Induce Regulatory Features in Human and Mouse CD1dhi B Cells: Inhibition of Allergic Inflammation by IL-10 and Regulatory T Cells

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0030883

    Presence of IL-10-producing CD1d hi B cells during S. haematobium (S.h.) infection. (A) The CD1d hi B cells and the IL-10 production of the total B cells were gated according to the gating strategy depicted in this graph. The gating of the IL-10 production of the different Breg subsets is similar as the total B cell gating. (B) PBMC were fixed and stained for Breg markers, including CD1d, and analyzed by flow cytometry. (C) Total peripheral blood B cells were cultured with anti-IgG/IgM or (D) anti-IgG/IgM plus SEA (10 µg/ml) for two days. Intracellular IL-10 production of the total B cells and the CD1d + B cells was determined following PMA/Ionomycin/LPS and BrefA stimulation.
    Figure Legend Snippet: Presence of IL-10-producing CD1d hi B cells during S. haematobium (S.h.) infection. (A) The CD1d hi B cells and the IL-10 production of the total B cells were gated according to the gating strategy depicted in this graph. The gating of the IL-10 production of the different Breg subsets is similar as the total B cell gating. (B) PBMC were fixed and stained for Breg markers, including CD1d, and analyzed by flow cytometry. (C) Total peripheral blood B cells were cultured with anti-IgG/IgM or (D) anti-IgG/IgM plus SEA (10 µg/ml) for two days. Intracellular IL-10 production of the total B cells and the CD1d + B cells was determined following PMA/Ionomycin/LPS and BrefA stimulation.

    Techniques Used: Infection, Staining, Flow Cytometry, Cytometry, Cell Culture

    3) Product Images from "Regulatory B cell frequency correlates with markers of HIV disease progression and attenuates anti-HIV CD8+ T cell function in vitro"

    Article Title: Regulatory B cell frequency correlates with markers of HIV disease progression and attenuates anti-HIV CD8+ T cell function in vitro

    Journal: Journal of Leukocyte Biology

    doi: 10.1189/jlb.0912436

    TLR and CD40 costimulation of Bregs lead to a higher frequency of IL-10 + cells and up-regulation of PD-L1 expression. CD19 + CD24 hi CD38 hi (Bregs)- and CD19 + CD24 lo CD38 lo (non-Bregs; mature B cell subset; A)-purified cells from healthy controls were costimulated with TLR2, TLR9, and CD40L for 48 h; for the final 5 h, the cultures were supplemented with PIB, and expression of intracellular IL-10 (B, left) and surface PD-L1 (B, right) was determined by flow cytometry. Representative diagrams from at least two independent experiments are shown. Numbers in quadrants indicate percentages. SSC-A, Side-scatter-area; FSC-A, forward-scatter-area; MFI, mean fluorescence intensity; PIB = PMA, (50ng/ml) Ionomycin (1μg/ml) and Brefeldin A (1:100).
    Figure Legend Snippet: TLR and CD40 costimulation of Bregs lead to a higher frequency of IL-10 + cells and up-regulation of PD-L1 expression. CD19 + CD24 hi CD38 hi (Bregs)- and CD19 + CD24 lo CD38 lo (non-Bregs; mature B cell subset; A)-purified cells from healthy controls were costimulated with TLR2, TLR9, and CD40L for 48 h; for the final 5 h, the cultures were supplemented with PIB, and expression of intracellular IL-10 (B, left) and surface PD-L1 (B, right) was determined by flow cytometry. Representative diagrams from at least two independent experiments are shown. Numbers in quadrants indicate percentages. SSC-A, Side-scatter-area; FSC-A, forward-scatter-area; MFI, mean fluorescence intensity; PIB = PMA, (50ng/ml) Ionomycin (1μg/ml) and Brefeldin A (1:100).

    Techniques Used: Expressing, Purification, Flow Cytometry, Cytometry, Fluorescence

    4) Product Images from "Tim-3 alters the balance of IL-12/IL-23 and drives TH17 cells: role in hepatitis B vaccine failure during hepatitis C infection"

    Article Title: Tim-3 alters the balance of IL-12/IL-23 and drives TH17 cells: role in hepatitis B vaccine failure during hepatitis C infection

    Journal: Vaccine

    doi: 10.1016/j.vaccine.2013.03.003

    T H 17 cells are significantly up-regulated in HCV-infected HBV-NR and are associated with Tim-3 expression and differential regulation of IL-23/IL-12 production by monocytes PBMC from HCV-infected HBV-NR and HBV-R or HS were stimulated with PMA/ionomycin for 6h, immune stained with CD4 and IL-17A, followed by flow cytometry analysis. A) Representative dot plots of IL-17A expression in CD4 + T cells without stimulation and stimulated CD4 + T cells from HCV-infected HBV-NR versus HBV-R or HS are shown on the left; and summary data of percentages of the positive cell frequency and MFI of IL-17A expression level in CD4 + cells from each group is shown on the right. *P
    Figure Legend Snippet: T H 17 cells are significantly up-regulated in HCV-infected HBV-NR and are associated with Tim-3 expression and differential regulation of IL-23/IL-12 production by monocytes PBMC from HCV-infected HBV-NR and HBV-R or HS were stimulated with PMA/ionomycin for 6h, immune stained with CD4 and IL-17A, followed by flow cytometry analysis. A) Representative dot plots of IL-17A expression in CD4 + T cells without stimulation and stimulated CD4 + T cells from HCV-infected HBV-NR versus HBV-R or HS are shown on the left; and summary data of percentages of the positive cell frequency and MFI of IL-17A expression level in CD4 + cells from each group is shown on the right. *P

    Techniques Used: Infection, Expressing, Staining, Flow Cytometry, Cytometry

    5) Product Images from "Distinct Pathways of Humoral and Cellular Immunity Induced with the Mucosal Administration of a Nanoemulsion Adjuvant"

    Article Title: Distinct Pathways of Humoral and Cellular Immunity Induced with the Mucosal Administration of a Nanoemulsion Adjuvant

    Journal: The Journal of Immunology Author Choice

    doi: 10.4049/jimmunol.1301424

    W 80 5EC NE promotes DC maturation phenotype in vitro. Human MDDCs were incubated for 48 h with 0.0001% or 0.001% NE, as indicated. Cells cultured with no treatment (Control) or incubated in the presence of PMA/ionomycin were used as activation controls.
    Figure Legend Snippet: W 80 5EC NE promotes DC maturation phenotype in vitro. Human MDDCs were incubated for 48 h with 0.0001% or 0.001% NE, as indicated. Cells cultured with no treatment (Control) or incubated in the presence of PMA/ionomycin were used as activation controls.

    Techniques Used: In Vitro, Incubation, Cell Culture, Activation Assay

    6) Product Images from "In Vivo Blockade of Murine ARTC2.2 During Cell Preparation Preserves the Vitality and Function of Liver Tissue-Resident Memory T Cells"

    Article Title: In Vivo Blockade of Murine ARTC2.2 During Cell Preparation Preserves the Vitality and Function of Liver Tissue-Resident Memory T Cells

    Journal: Frontiers in Immunology

    doi: 10.3389/fimmu.2018.01580

    In vitro cytokine expression profile of PMA/ionomycin stimulated liver Trm. (A) CD8 + (1 × 10 4 cells) and CD4 + (2 × 10 4 cells) Trm from the liver of Lm infected mice were FACS sorted 7 weeks after infection. One group of mice was treated with s+16a prior to organ harvesting and the second group was left untreated as control. Cells were stimulated with PMA/ionomycin for 20 h and T cell cytokines [IFNγ, TNFα, interleukin (IL)-2, IL-4, IL-21, IL-22, IL-17A, IL-17F, IL-10, IL-9, IL-5, and IL-13] were measured in the supernatants using a bead-based 13-plex assay. (B) The concentration of cytokines in the supernatants of stimulated CD4 + and CD8 + Trm from s+16a-treated and control mice ( n = 3) was quantified.
    Figure Legend Snippet: In vitro cytokine expression profile of PMA/ionomycin stimulated liver Trm. (A) CD8 + (1 × 10 4 cells) and CD4 + (2 × 10 4 cells) Trm from the liver of Lm infected mice were FACS sorted 7 weeks after infection. One group of mice was treated with s+16a prior to organ harvesting and the second group was left untreated as control. Cells were stimulated with PMA/ionomycin for 20 h and T cell cytokines [IFNγ, TNFα, interleukin (IL)-2, IL-4, IL-21, IL-22, IL-17A, IL-17F, IL-10, IL-9, IL-5, and IL-13] were measured in the supernatants using a bead-based 13-plex assay. (B) The concentration of cytokines in the supernatants of stimulated CD4 + and CD8 + Trm from s+16a-treated and control mice ( n = 3) was quantified.

    Techniques Used: In Vitro, Expressing, Infection, Mouse Assay, FACS, Plex Assay, Concentration Assay

    7) Product Images from "Regulatory B Cells Inhibit Cytotoxic T Lymphocyte (CTL) Activity and Elimination of Infected CD4 T Cells after In Vitro Reactivation of HIV Latent Reservoirs"

    Article Title: Regulatory B Cells Inhibit Cytotoxic T Lymphocyte (CTL) Activity and Elimination of Infected CD4 T Cells after In Vitro Reactivation of HIV Latent Reservoirs

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0092934

    HIV EC and HIV NEG have comparable Bregs frequency. PBMC from HIV EC (n = 15), HIV ART (n = 20), HIV NEG , (n = 20) and HIV VIR , (n = 17) were cultured for 48H and during the final 5H supplemented with PMA (25 ng/ml), Ionomycin (1 ug/ml), Brefeldin A (1∶100) and by flow cytometry, the ( a,b ) frequency of CD19 + CD24 hi CD38 hi Bregs and ( c ) IL-10-positive Bregs (intracellular cytokine staining) determined. p values for differences as calculated by Mann Whitney test two-tailed t test (Graphpad Prism software) are indicated; * = p
    Figure Legend Snippet: HIV EC and HIV NEG have comparable Bregs frequency. PBMC from HIV EC (n = 15), HIV ART (n = 20), HIV NEG , (n = 20) and HIV VIR , (n = 17) were cultured for 48H and during the final 5H supplemented with PMA (25 ng/ml), Ionomycin (1 ug/ml), Brefeldin A (1∶100) and by flow cytometry, the ( a,b ) frequency of CD19 + CD24 hi CD38 hi Bregs and ( c ) IL-10-positive Bregs (intracellular cytokine staining) determined. p values for differences as calculated by Mann Whitney test two-tailed t test (Graphpad Prism software) are indicated; * = p

    Techniques Used: Cell Culture, Flow Cytometry, Cytometry, Staining, MANN-WHITNEY, Two Tailed Test, Software

    8) Product Images from "CD161 defines effector T cells that express light and respond to TL1A-DR3 signaling"

    Article Title: CD161 defines effector T cells that express light and respond to TL1A-DR3 signaling

    Journal: European Journal of Microbiology & Immunology

    doi: 10.1556/EuJMI.1.2011.1.9

    LIGHT is preferentially expressed on CD4 + T cellsco-expressing CD161. PBL were isolated from a healthy donor blood andcultured for 18 hours in the presence of PMA and Ionomycin. Cells weresurface stained with anti-CD3, anti-CD4/8, anti-CD161 and anti-LIGHTantibodies
    Figure Legend Snippet: LIGHT is preferentially expressed on CD4 + T cellsco-expressing CD161. PBL were isolated from a healthy donor blood andcultured for 18 hours in the presence of PMA and Ionomycin. Cells weresurface stained with anti-CD3, anti-CD4/8, anti-CD161 and anti-LIGHTantibodies

    Techniques Used: Expressing, Isolation, Staining

    9) Product Images from "Characterisation of mice lacking all functional isoforms of the pro-survival BCL-2 family member A1 reveals minor defects in the haematopoietic compartment"

    Article Title: Characterisation of mice lacking all functional isoforms of the pro-survival BCL-2 family member A1 reveals minor defects in the haematopoietic compartment

    Journal: Cell Death and Differentiation

    doi: 10.1038/cdd.2016.156

    T cell development and stress-induced apoptosis are normal in A1 −/− mice. ( a , left) Representative FACS plots of developing T cells in the thymus of wild-type and A1 −/− mice. T cells develop in the thymus from the CD4/CD8 double-negative (DN) stage, becoming double positive (DP) and then differentiating into either CD4 or CD8 single-positive cells. The DN population is further subdivided into DN1–4 phases: DN1 (CD44 + CD25 − ), DN2 (CD44 + CD25 + ), DN3 (CD44 − CD25 + ) and DN4 (CD44 − CD25 − ). ( a , right) Quantified total cell numbers for developing T cell populations in wild-type and A1 −/− mice. ( b ) Thymocytes harvested from wild-type and A1 −/− mice were put into culture with the following apoptosis-inducing compounds: ABT-737 (737) 0.5 μ M, dexamethasone (Dexa) 0.5 ng/ml, etoposide (Etopo) 0.5 μ g/ml, ionomycin (Iono), 0.1 μ g/ml, PMA 0.5 ng/ml or DMSO as a control. Apoptosis was assessed by FACS analysis after staining with fluorochrome-conjugated Annexin-V and PI (5 μ g/ml) at 24 h post treatment. Data are representative of six mice for each genotype, with each treatment performed in triplicate
    Figure Legend Snippet: T cell development and stress-induced apoptosis are normal in A1 −/− mice. ( a , left) Representative FACS plots of developing T cells in the thymus of wild-type and A1 −/− mice. T cells develop in the thymus from the CD4/CD8 double-negative (DN) stage, becoming double positive (DP) and then differentiating into either CD4 or CD8 single-positive cells. The DN population is further subdivided into DN1–4 phases: DN1 (CD44 + CD25 − ), DN2 (CD44 + CD25 + ), DN3 (CD44 − CD25 + ) and DN4 (CD44 − CD25 − ). ( a , right) Quantified total cell numbers for developing T cell populations in wild-type and A1 −/− mice. ( b ) Thymocytes harvested from wild-type and A1 −/− mice were put into culture with the following apoptosis-inducing compounds: ABT-737 (737) 0.5 μ M, dexamethasone (Dexa) 0.5 ng/ml, etoposide (Etopo) 0.5 μ g/ml, ionomycin (Iono), 0.1 μ g/ml, PMA 0.5 ng/ml or DMSO as a control. Apoptosis was assessed by FACS analysis after staining with fluorochrome-conjugated Annexin-V and PI (5 μ g/ml) at 24 h post treatment. Data are representative of six mice for each genotype, with each treatment performed in triplicate

    Techniques Used: Mouse Assay, FACS, Staining

    10) Product Images from "A P2rx7 passenger mutation affects the vitality and function of immune cells in P2X4ko and other transgenic mice"

    Article Title: A P2rx7 passenger mutation affects the vitality and function of immune cells in P2X4ko and other transgenic mice

    Journal: bioRxiv

    doi: 10.1101/2020.06.10.139410

    The enhanced NICD of B6-P2X4ko T cells can lead to misinterpretation of functional assays. (A) B6 WT and B6 P2X4ko mice were injected or not with anti-ARTC2.2 nanobody s+16a in order to prevent P2X7 ADP-ribosylation during cell preparation. CTL and Th from spleen were FACS sorted and obtained cells were incubated at 37°C for 2h or left at 4°C. Cell vitality was measured by propidium iodide (PI) uptake (n = 3). (B) B6 WT and P2X4ko mice were injected (s+16a) or not (control) with anti-ARTC2.2 nanobody s+16a. 5 x 10 4 CTL were FACS sorted and stimulated ex vivo with PMA/ionomycin for 20h. IL-2, IFNγ and TNFα in the supernatants were measured by cytometric bead array. (C) 1×10 5 Th from P2X4ko mice (B6, B6 treated with s+16a or Balb/c) and 1×10 5 eFluor 670 labeled Th from corresponding WT mice were mixed and transferred to the top well of a trans-well chamber in order to perform a comparative migration assay towards SDF1α, which was used as chemoattractant in the bottom well. Migration of Th into the bottom was determined by counting living cells in the top and bottom well after 2h of incubation at 37°C. (D) Comparative adoptive transfer of CTL obtained from B6 WT and P2X4ko mice that have been treated or not with s+16a was performed. Cells were differentially labeled with eFluor 670 and eFluor 450 and 4×10 5 cells mixed in a 1:1 ratio were i.v. injected into RAG1ko mice. Spleens were analyzed after 24h for the presence of transferred mixed cells. Statistical comparison of two groups was performed by using the student’s t test (p
    Figure Legend Snippet: The enhanced NICD of B6-P2X4ko T cells can lead to misinterpretation of functional assays. (A) B6 WT and B6 P2X4ko mice were injected or not with anti-ARTC2.2 nanobody s+16a in order to prevent P2X7 ADP-ribosylation during cell preparation. CTL and Th from spleen were FACS sorted and obtained cells were incubated at 37°C for 2h or left at 4°C. Cell vitality was measured by propidium iodide (PI) uptake (n = 3). (B) B6 WT and P2X4ko mice were injected (s+16a) or not (control) with anti-ARTC2.2 nanobody s+16a. 5 x 10 4 CTL were FACS sorted and stimulated ex vivo with PMA/ionomycin for 20h. IL-2, IFNγ and TNFα in the supernatants were measured by cytometric bead array. (C) 1×10 5 Th from P2X4ko mice (B6, B6 treated with s+16a or Balb/c) and 1×10 5 eFluor 670 labeled Th from corresponding WT mice were mixed and transferred to the top well of a trans-well chamber in order to perform a comparative migration assay towards SDF1α, which was used as chemoattractant in the bottom well. Migration of Th into the bottom was determined by counting living cells in the top and bottom well after 2h of incubation at 37°C. (D) Comparative adoptive transfer of CTL obtained from B6 WT and P2X4ko mice that have been treated or not with s+16a was performed. Cells were differentially labeled with eFluor 670 and eFluor 450 and 4×10 5 cells mixed in a 1:1 ratio were i.v. injected into RAG1ko mice. Spleens were analyzed after 24h for the presence of transferred mixed cells. Statistical comparison of two groups was performed by using the student’s t test (p

    Techniques Used: Functional Assay, Mouse Assay, Injection, FACS, Incubation, Ex Vivo, Labeling, Migration, Adoptive Transfer Assay

    Related Articles

    Isolation:

    Article Title: In Vivo Blockade of Murine ARTC2.2 During Cell Preparation Preserves the Vitality and Function of Liver Tissue-Resident Memory T Cells
    Article Snippet: .. Isolated Trm were cultured in 200 µl IMDM (ThermoFisher) + 5% FCS, β-mercaptoethanol (50 µM, ThermoFisher), and gentamicin (50 µg/ml, ThermoFisher) at a cell density of 20,000 (CD4+ Trm) or 10,000 (CD8+ Trm) cells per well for 20 h in the presence of Phorbol 12-myristate 13-acetate (PMA, 20 ng/ml, Invivogen) and ionomycin (1 µg/ml, Invivogen) to induce cytokine expression. .. Levels of IFN-γ, TNF-α, IL-2, IL-4, IL-21, IL-22, IL-17A, IL-17F, IL-10, IL-9, IL-5, and IL-13 were measured in the supernatants of stimulated Trm by using the LEGENDplex mouse Th cytokine 13-plex (BioLegend) according to the manufacture’s instruction.

    Cell Culture:

    Article Title: In Vivo Blockade of Murine ARTC2.2 During Cell Preparation Preserves the Vitality and Function of Liver Tissue-Resident Memory T Cells
    Article Snippet: .. Isolated Trm were cultured in 200 µl IMDM (ThermoFisher) + 5% FCS, β-mercaptoethanol (50 µM, ThermoFisher), and gentamicin (50 µg/ml, ThermoFisher) at a cell density of 20,000 (CD4+ Trm) or 10,000 (CD8+ Trm) cells per well for 20 h in the presence of Phorbol 12-myristate 13-acetate (PMA, 20 ng/ml, Invivogen) and ionomycin (1 µg/ml, Invivogen) to induce cytokine expression. .. Levels of IFN-γ, TNF-α, IL-2, IL-4, IL-21, IL-22, IL-17A, IL-17F, IL-10, IL-9, IL-5, and IL-13 were measured in the supernatants of stimulated Trm by using the LEGENDplex mouse Th cytokine 13-plex (BioLegend) according to the manufacture’s instruction.

    Article Title: Regulatory B Cells Inhibit Cytotoxic T Lymphocyte (CTL) Activity and Elimination of Infected CD4 T Cells after In Vitro Reactivation of HIV Latent Reservoirs
    Article Snippet: .. Analysis of IL-10 Production by Bregs and Immunophenotyping of PBMCs To determine endogenous intracellular IL-10 production, PBMC were cultured for 48 hours; during the final 5 hours of incubation the cultures were supplemented with Brefeldin A (1∶100, BD), PMA (50 ng/ml, Invivogen) and Ionomycin (1 ug/ml, Invivogen). .. After incubation the cells were washed, stained for viable cells (LIVE/DEAD Aqua Fixable Dead Cell Stain Kit, Invitrogen), surface stained, fixed/permeabilized (Fix/Perm Kit BD Biosciences) and stained for intracellular IL-10 (IL-10-AF-647, eBioscience).

    Produced:

    Article Title: Characterisation of mice lacking all functional isoforms of the pro-survival BCL-2 family member A1 reveals minor defects in the haematopoietic compartment
    Article Snippet: .. For ionomycin, LPS and IL-33 treatment survival assays, cells were washed to remove cytokines and then treated with 1 μ g/ml ionomycin, 5 μ g/ml ultrapure LPS ( E. coli 0111:B4 strain–TLR4 ligand, InvivoGen, San Diego, CA, USA), 10 ng/ml IL-33 (produced in-house and kindly provided by Dr. Ajithkumar Vasanthakumar of WEHI) or medium alone (no cytokines added). ..

    Incubation:

    Article Title: Cellular barcodes increase the efficiency of profiling single-cell secretory responses by microengraving
    Article Snippet: .. To compare diverse stimulation conditions, PBMCs (106 cells/well) were incubated in a 96-well U-bottom plate in media only or stimulated for 4 h with PMA/ionomycin (10 ng/mL PMA, 1 µM ionomycin), the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS-EK, 1 µg/mL; InvivoGen, San Diego, CA), or the TLR7/8 agonist R848 (1 µg/mL; InvivoGen). .. To test CD4+ Th cell lineages, Th0-, Th1-, or Th2-polarized cells (105 cells/well) were incubated in a 96-well U-bottom plate in media only or stimulated for 4 h with PMA/ionomycin (10 ng/mL PMA, 1 µg/mL ionomycin) as indicated.

    Article Title: Regulatory B Cells Inhibit Cytotoxic T Lymphocyte (CTL) Activity and Elimination of Infected CD4 T Cells after In Vitro Reactivation of HIV Latent Reservoirs
    Article Snippet: .. Analysis of IL-10 Production by Bregs and Immunophenotyping of PBMCs To determine endogenous intracellular IL-10 production, PBMC were cultured for 48 hours; during the final 5 hours of incubation the cultures were supplemented with Brefeldin A (1∶100, BD), PMA (50 ng/ml, Invivogen) and Ionomycin (1 ug/ml, Invivogen). .. After incubation the cells were washed, stained for viable cells (LIVE/DEAD Aqua Fixable Dead Cell Stain Kit, Invitrogen), surface stained, fixed/permeabilized (Fix/Perm Kit BD Biosciences) and stained for intracellular IL-10 (IL-10-AF-647, eBioscience).

    Article Title: Regulatory B cell frequency correlates with markers of HIV disease progression and attenuates anti-HIV CD8+ T cell function in vitro
    Article Snippet: .. During the final 5 h of incubation, the cultures were supplemented with PIB (PMA, (50ng/ml) Ionomycin (1μg/ml) and Brefeldin A (1:1000); InvivoGen and BD Biosciences). .. After incubation, the cells were washed, stained for viable cells (LIVE/DEAD fixable aqua dead cell stain kit; Invitrogen, Life Technologies, Carlsbad, CA, USA), surface-stained, fixed/permeabilized (Fix/Perm kit; BD Biosciences), and stained for intracellular IL-10 (IL-10-AF-647; eBioscience, San Diego, CA, USA).

    Article Title: Schistosomes Induce Regulatory Features in Human and Mouse CD1dhi B Cells: Inhibition of Allergic Inflammation by IL-10 and Regulatory T Cells
    Article Snippet: .. For ICS of IL-10, B cells were restimulated with PMA (50 ng/ml), ionomycin (2 µg/ml), and LPS (100 ng/ml; Invivogen) for 6 hours with the final 4 hours in the presence of BrefA (10 µg/ml; Sigma-Aldrich), followed by fixation with FoxP3 fixation/permeabilization kit and stained for CD1d-PE (51.1, eBioscience), CD20-APCeFluor780 (2H7, eBioscience), and IL-10-biotin (JES3-12G8, Abd Serotec) followed by second incubation with streptavidin-Qdot525 (Invitrogen). .. Statistical analysis All murine results are expressed as mean ± SEM and the different groups were tested using the Student's t -test (two-tailed).

    Expressing:

    Article Title: In Vivo Blockade of Murine ARTC2.2 During Cell Preparation Preserves the Vitality and Function of Liver Tissue-Resident Memory T Cells
    Article Snippet: .. Isolated Trm were cultured in 200 µl IMDM (ThermoFisher) + 5% FCS, β-mercaptoethanol (50 µM, ThermoFisher), and gentamicin (50 µg/ml, ThermoFisher) at a cell density of 20,000 (CD4+ Trm) or 10,000 (CD8+ Trm) cells per well for 20 h in the presence of Phorbol 12-myristate 13-acetate (PMA, 20 ng/ml, Invivogen) and ionomycin (1 µg/ml, Invivogen) to induce cytokine expression. .. Levels of IFN-γ, TNF-α, IL-2, IL-4, IL-21, IL-22, IL-17A, IL-17F, IL-10, IL-9, IL-5, and IL-13 were measured in the supernatants of stimulated Trm by using the LEGENDplex mouse Th cytokine 13-plex (BioLegend) according to the manufacture’s instruction.

    Staining:

    Article Title: Schistosomes Induce Regulatory Features in Human and Mouse CD1dhi B Cells: Inhibition of Allergic Inflammation by IL-10 and Regulatory T Cells
    Article Snippet: .. For ICS of IL-10, B cells were restimulated with PMA (50 ng/ml), ionomycin (2 µg/ml), and LPS (100 ng/ml; Invivogen) for 6 hours with the final 4 hours in the presence of BrefA (10 µg/ml; Sigma-Aldrich), followed by fixation with FoxP3 fixation/permeabilization kit and stained for CD1d-PE (51.1, eBioscience), CD20-APCeFluor780 (2H7, eBioscience), and IL-10-biotin (JES3-12G8, Abd Serotec) followed by second incubation with streptavidin-Qdot525 (Invitrogen). .. Statistical analysis All murine results are expressed as mean ± SEM and the different groups were tested using the Student's t -test (two-tailed).

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 96
    InvivoGen ionomycin
    T cell development and stress-induced apoptosis are normal in A1 −/− mice. ( a , left) Representative FACS plots of developing T cells in the thymus of wild-type and A1 −/− mice. T cells develop in the thymus from the CD4/CD8 double-negative (DN) stage, becoming double positive (DP) and then differentiating into either CD4 or CD8 single-positive cells. The DN population is further subdivided into DN1–4 phases: DN1 (CD44 + CD25 − ), DN2 (CD44 + CD25 + ), DN3 (CD44 − CD25 + ) and DN4 (CD44 − CD25 − ). ( a , right) Quantified total cell numbers for developing T cell populations in wild-type and A1 −/− mice. ( b ) Thymocytes harvested from wild-type and A1 −/− mice were put into culture with the following apoptosis-inducing compounds: ABT-737 (737) 0.5 μ M, dexamethasone (Dexa) 0.5 ng/ml, etoposide (Etopo) 0.5 μ g/ml, <t>ionomycin</t> (Iono), 0.1 μ g/ml, PMA 0.5 ng/ml or DMSO as a control. Apoptosis was assessed by FACS analysis after staining with fluorochrome-conjugated Annexin-V and PI (5 μ g/ml) at 24 h post treatment. Data are representative of six mice for each genotype, with each treatment performed in triplicate
    Ionomycin, supplied by InvivoGen, used in various techniques. Bioz Stars score: 96/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ionomycin/product/InvivoGen
    Average 96 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    ionomycin - by Bioz Stars, 2020-09
    96/100 stars
      Buy from Supplier

    Image Search Results


    T cell development and stress-induced apoptosis are normal in A1 −/− mice. ( a , left) Representative FACS plots of developing T cells in the thymus of wild-type and A1 −/− mice. T cells develop in the thymus from the CD4/CD8 double-negative (DN) stage, becoming double positive (DP) and then differentiating into either CD4 or CD8 single-positive cells. The DN population is further subdivided into DN1–4 phases: DN1 (CD44 + CD25 − ), DN2 (CD44 + CD25 + ), DN3 (CD44 − CD25 + ) and DN4 (CD44 − CD25 − ). ( a , right) Quantified total cell numbers for developing T cell populations in wild-type and A1 −/− mice. ( b ) Thymocytes harvested from wild-type and A1 −/− mice were put into culture with the following apoptosis-inducing compounds: ABT-737 (737) 0.5 μ M, dexamethasone (Dexa) 0.5 ng/ml, etoposide (Etopo) 0.5 μ g/ml, ionomycin (Iono), 0.1 μ g/ml, PMA 0.5 ng/ml or DMSO as a control. Apoptosis was assessed by FACS analysis after staining with fluorochrome-conjugated Annexin-V and PI (5 μ g/ml) at 24 h post treatment. Data are representative of six mice for each genotype, with each treatment performed in triplicate

    Journal: Cell Death and Differentiation

    Article Title: Characterisation of mice lacking all functional isoforms of the pro-survival BCL-2 family member A1 reveals minor defects in the haematopoietic compartment

    doi: 10.1038/cdd.2016.156

    Figure Lengend Snippet: T cell development and stress-induced apoptosis are normal in A1 −/− mice. ( a , left) Representative FACS plots of developing T cells in the thymus of wild-type and A1 −/− mice. T cells develop in the thymus from the CD4/CD8 double-negative (DN) stage, becoming double positive (DP) and then differentiating into either CD4 or CD8 single-positive cells. The DN population is further subdivided into DN1–4 phases: DN1 (CD44 + CD25 − ), DN2 (CD44 + CD25 + ), DN3 (CD44 − CD25 + ) and DN4 (CD44 − CD25 − ). ( a , right) Quantified total cell numbers for developing T cell populations in wild-type and A1 −/− mice. ( b ) Thymocytes harvested from wild-type and A1 −/− mice were put into culture with the following apoptosis-inducing compounds: ABT-737 (737) 0.5 μ M, dexamethasone (Dexa) 0.5 ng/ml, etoposide (Etopo) 0.5 μ g/ml, ionomycin (Iono), 0.1 μ g/ml, PMA 0.5 ng/ml or DMSO as a control. Apoptosis was assessed by FACS analysis after staining with fluorochrome-conjugated Annexin-V and PI (5 μ g/ml) at 24 h post treatment. Data are representative of six mice for each genotype, with each treatment performed in triplicate

    Article Snippet: For ionomycin, LPS and IL-33 treatment survival assays, cells were washed to remove cytokines and then treated with 1 μ g/ml ionomycin, 5 μ g/ml ultrapure LPS ( E. coli 0111:B4 strain–TLR4 ligand, InvivoGen, San Diego, CA, USA), 10 ng/ml IL-33 (produced in-house and kindly provided by Dr. Ajithkumar Vasanthakumar of WEHI) or medium alone (no cytokines added).

    Techniques: Mouse Assay, FACS, Staining

    Application of cellular barcodes to construct dose-response curves. Barcoded T cells were stimulated with 0, 0.25, 0.5, or 1 µg/mL ionomycin and 10 ng/mL PMA. Microengraving was used to measure single-cell secretory responses, and surface phenotype

    Journal: Analytical chemistry

    Article Title: Cellular barcodes increase the efficiency of profiling single-cell secretory responses by microengraving

    doi: 10.1021/ac302264q

    Figure Lengend Snippet: Application of cellular barcodes to construct dose-response curves. Barcoded T cells were stimulated with 0, 0.25, 0.5, or 1 µg/mL ionomycin and 10 ng/mL PMA. Microengraving was used to measure single-cell secretory responses, and surface phenotype

    Article Snippet: To compare diverse stimulation conditions, PBMCs (106 cells/well) were incubated in a 96-well U-bottom plate in media only or stimulated for 4 h with PMA/ionomycin (10 ng/mL PMA, 1 µM ionomycin), the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS-EK, 1 µg/mL; InvivoGen, San Diego, CA), or the TLR7/8 agonist R848 (1 µg/mL; InvivoGen).

    Techniques: Construct

    Application of cellular barcodes to measure the percentage of single PBMCs secreting cytokines after treatment with mechanistically distinct stimuli. Barcoded PBMCs were stimulated with PMA/ionomycin (P/I), R848, LPS, or left unstimulated (−).

    Journal: Analytical chemistry

    Article Title: Cellular barcodes increase the efficiency of profiling single-cell secretory responses by microengraving

    doi: 10.1021/ac302264q

    Figure Lengend Snippet: Application of cellular barcodes to measure the percentage of single PBMCs secreting cytokines after treatment with mechanistically distinct stimuli. Barcoded PBMCs were stimulated with PMA/ionomycin (P/I), R848, LPS, or left unstimulated (−).

    Article Snippet: To compare diverse stimulation conditions, PBMCs (106 cells/well) were incubated in a 96-well U-bottom plate in media only or stimulated for 4 h with PMA/ionomycin (10 ng/mL PMA, 1 µM ionomycin), the Toll-like receptor 4 (TLR4) agonist lipopolysaccharide (LPS-EK, 1 µg/mL; InvivoGen, San Diego, CA), or the TLR7/8 agonist R848 (1 µg/mL; InvivoGen).

    Techniques:

    Presence of IL-10-producing CD1d hi B cells during S. haematobium (S.h.) infection. (A) The CD1d hi B cells and the IL-10 production of the total B cells were gated according to the gating strategy depicted in this graph. The gating of the IL-10 production of the different Breg subsets is similar as the total B cell gating. (B) PBMC were fixed and stained for Breg markers, including CD1d, and analyzed by flow cytometry. (C) Total peripheral blood B cells were cultured with anti-IgG/IgM or (D) anti-IgG/IgM plus SEA (10 µg/ml) for two days. Intracellular IL-10 production of the total B cells and the CD1d + B cells was determined following PMA/Ionomycin/LPS and BrefA stimulation.

    Journal: PLoS ONE

    Article Title: Schistosomes Induce Regulatory Features in Human and Mouse CD1dhi B Cells: Inhibition of Allergic Inflammation by IL-10 and Regulatory T Cells

    doi: 10.1371/journal.pone.0030883

    Figure Lengend Snippet: Presence of IL-10-producing CD1d hi B cells during S. haematobium (S.h.) infection. (A) The CD1d hi B cells and the IL-10 production of the total B cells were gated according to the gating strategy depicted in this graph. The gating of the IL-10 production of the different Breg subsets is similar as the total B cell gating. (B) PBMC were fixed and stained for Breg markers, including CD1d, and analyzed by flow cytometry. (C) Total peripheral blood B cells were cultured with anti-IgG/IgM or (D) anti-IgG/IgM plus SEA (10 µg/ml) for two days. Intracellular IL-10 production of the total B cells and the CD1d + B cells was determined following PMA/Ionomycin/LPS and BrefA stimulation.

    Article Snippet: For ICS of IL-10, B cells were restimulated with PMA (50 ng/ml), ionomycin (2 µg/ml), and LPS (100 ng/ml; Invivogen) for 6 hours with the final 4 hours in the presence of BrefA (10 µg/ml; Sigma-Aldrich), followed by fixation with FoxP3 fixation/permeabilization kit and stained for CD1d-PE (51.1, eBioscience), CD20-APCeFluor780 (2H7, eBioscience), and IL-10-biotin (JES3-12G8, Abd Serotec) followed by second incubation with streptavidin-Qdot525 (Invitrogen).

    Techniques: Infection, Staining, Flow Cytometry, Cytometry, Cell Culture

    TLR and CD40 costimulation of Bregs lead to a higher frequency of IL-10 + cells and up-regulation of PD-L1 expression. CD19 + CD24 hi CD38 hi (Bregs)- and CD19 + CD24 lo CD38 lo (non-Bregs; mature B cell subset; A)-purified cells from healthy controls were costimulated with TLR2, TLR9, and CD40L for 48 h; for the final 5 h, the cultures were supplemented with PIB, and expression of intracellular IL-10 (B, left) and surface PD-L1 (B, right) was determined by flow cytometry. Representative diagrams from at least two independent experiments are shown. Numbers in quadrants indicate percentages. SSC-A, Side-scatter-area; FSC-A, forward-scatter-area; MFI, mean fluorescence intensity; PIB = PMA, (50ng/ml) Ionomycin (1μg/ml) and Brefeldin A (1:100).

    Journal: Journal of Leukocyte Biology

    Article Title: Regulatory B cell frequency correlates with markers of HIV disease progression and attenuates anti-HIV CD8+ T cell function in vitro

    doi: 10.1189/jlb.0912436

    Figure Lengend Snippet: TLR and CD40 costimulation of Bregs lead to a higher frequency of IL-10 + cells and up-regulation of PD-L1 expression. CD19 + CD24 hi CD38 hi (Bregs)- and CD19 + CD24 lo CD38 lo (non-Bregs; mature B cell subset; A)-purified cells from healthy controls were costimulated with TLR2, TLR9, and CD40L for 48 h; for the final 5 h, the cultures were supplemented with PIB, and expression of intracellular IL-10 (B, left) and surface PD-L1 (B, right) was determined by flow cytometry. Representative diagrams from at least two independent experiments are shown. Numbers in quadrants indicate percentages. SSC-A, Side-scatter-area; FSC-A, forward-scatter-area; MFI, mean fluorescence intensity; PIB = PMA, (50ng/ml) Ionomycin (1μg/ml) and Brefeldin A (1:100).

    Article Snippet: During the final 5 h of incubation, the cultures were supplemented with PIB (PMA, (50ng/ml) Ionomycin (1μg/ml) and Brefeldin A (1:1000); InvivoGen and BD Biosciences).

    Techniques: Expressing, Purification, Flow Cytometry, Cytometry, Fluorescence