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Calbiotech ionomycin
IFN-γ-mediated protection prevents IL-17-mediated mortality. (A) Number of CD4 + T cells in the infiltrating population and distribution of Thy1.1 positive cells measured by flow cytometry at day eight p.i. Data represent means (±SD) of twelve mice per group combined from three separate experiments. (B) IL-17 mRNA expression determined by quantitative real-time PCR in infected SCID recipients of WT, WT/GKO or GKO CD4 + T cells. Data represent the mean of two experiments with n = 4 in each group per experiment. (C) Splenocytes of immunized GKO donors cultured in the presence of JHMV with or without recombinant IFN-γ (10 ng/ml) for six days and restimulated four hours with <t>PMA/Ionomycin.</t> Intracellular cytokine expression on CD4 + T cells analyzed by flow cytometry using FITC-IFN-γ, PE-IL-17 and the corresponding isotype controls. Dot plots are representative of duplicates from two separate experiments. (D) Survival of infected SCID recipients of WT/GKO (n = 6), WT/GKO + anti-IFN-γ mAb (n = 8) and GKO (n = 4) CD4 + T cells assessed daily. Data are representative of two separate experiments.
Ionomycin, supplied by Calbiotech, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ionomycin/product/Calbiotech
Average 92 stars, based on 3 article reviews
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ionomycin - by Bioz Stars, 2020-09
92/100 stars

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1) Product Images from "IFN-? protects from lethal IL-17 mediated viral encephalomyelitis independent of neutrophils"

Article Title: IFN-? protects from lethal IL-17 mediated viral encephalomyelitis independent of neutrophils

Journal: Journal of Neuroinflammation

doi: 10.1186/1742-2094-9-104

IFN-γ-mediated protection prevents IL-17-mediated mortality. (A) Number of CD4 + T cells in the infiltrating population and distribution of Thy1.1 positive cells measured by flow cytometry at day eight p.i. Data represent means (±SD) of twelve mice per group combined from three separate experiments. (B) IL-17 mRNA expression determined by quantitative real-time PCR in infected SCID recipients of WT, WT/GKO or GKO CD4 + T cells. Data represent the mean of two experiments with n = 4 in each group per experiment. (C) Splenocytes of immunized GKO donors cultured in the presence of JHMV with or without recombinant IFN-γ (10 ng/ml) for six days and restimulated four hours with PMA/Ionomycin. Intracellular cytokine expression on CD4 + T cells analyzed by flow cytometry using FITC-IFN-γ, PE-IL-17 and the corresponding isotype controls. Dot plots are representative of duplicates from two separate experiments. (D) Survival of infected SCID recipients of WT/GKO (n = 6), WT/GKO + anti-IFN-γ mAb (n = 8) and GKO (n = 4) CD4 + T cells assessed daily. Data are representative of two separate experiments.
Figure Legend Snippet: IFN-γ-mediated protection prevents IL-17-mediated mortality. (A) Number of CD4 + T cells in the infiltrating population and distribution of Thy1.1 positive cells measured by flow cytometry at day eight p.i. Data represent means (±SD) of twelve mice per group combined from three separate experiments. (B) IL-17 mRNA expression determined by quantitative real-time PCR in infected SCID recipients of WT, WT/GKO or GKO CD4 + T cells. Data represent the mean of two experiments with n = 4 in each group per experiment. (C) Splenocytes of immunized GKO donors cultured in the presence of JHMV with or without recombinant IFN-γ (10 ng/ml) for six days and restimulated four hours with PMA/Ionomycin. Intracellular cytokine expression on CD4 + T cells analyzed by flow cytometry using FITC-IFN-γ, PE-IL-17 and the corresponding isotype controls. Dot plots are representative of duplicates from two separate experiments. (D) Survival of infected SCID recipients of WT/GKO (n = 6), WT/GKO + anti-IFN-γ mAb (n = 8) and GKO (n = 4) CD4 + T cells assessed daily. Data are representative of two separate experiments.

Techniques Used: Flow Cytometry, Cytometry, Mouse Assay, Expressing, Real-time Polymerase Chain Reaction, Infection, Cell Culture, Recombinant

Related Articles

Ex Vivo:

Article Title: IFN-? protects from lethal IL-17 mediated viral encephalomyelitis independent of neutrophils
Article Snippet: .. Cytokine production from both splenic cultures or ex vivo lymph node cells was measured following four hours stimulation with PMA (10 ng/ml) (Acros Organics, Geel, Belgium) and ionomycin (1 μM) (Calbiotech, Spring Valley, CA, USA). .. Monensin (2 μM) (Calbiotech) was added to the cultures for the last two hours.

Lysis:

Article Title: Transcription factor NFAT1 controls allergic contact hypersensitivity through regulation of activation induced cell death program
Article Snippet: .. After 24 hrs, cells were stimulated with PMA (Calbiotech, CA, USA, CA, USA) and ionomycin (Calbiotech, CA, USA) for 4 hrs, collected and lysed in passive lysis buffer (Promega, Madison, WI, USA). .. Luciferase activity measured by dual luciferase assay system (Promega, Madison, WI, USA) was expressed relative to expression of the co-transfected Renilla luciferase promoter (hRluc; Promega, Madison, WI, USA) as control for transfection efficiency.

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    Calbiotech ionomycin
    IFN-γ-mediated protection prevents IL-17-mediated mortality. (A) Number of CD4 + T cells in the infiltrating population and distribution of Thy1.1 positive cells measured by flow cytometry at day eight p.i. Data represent means (±SD) of twelve mice per group combined from three separate experiments. (B) IL-17 mRNA expression determined by quantitative real-time PCR in infected SCID recipients of WT, WT/GKO or GKO CD4 + T cells. Data represent the mean of two experiments with n = 4 in each group per experiment. (C) Splenocytes of immunized GKO donors cultured in the presence of JHMV with or without recombinant IFN-γ (10 ng/ml) for six days and restimulated four hours with <t>PMA/Ionomycin.</t> Intracellular cytokine expression on CD4 + T cells analyzed by flow cytometry using FITC-IFN-γ, PE-IL-17 and the corresponding isotype controls. Dot plots are representative of duplicates from two separate experiments. (D) Survival of infected SCID recipients of WT/GKO (n = 6), WT/GKO + anti-IFN-γ mAb (n = 8) and GKO (n = 4) CD4 + T cells assessed daily. Data are representative of two separate experiments.
    Ionomycin, supplied by Calbiotech, used in various techniques. Bioz Stars score: 92/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ionomycin/product/Calbiotech
    Average 92 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    ionomycin - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

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    IFN-γ-mediated protection prevents IL-17-mediated mortality. (A) Number of CD4 + T cells in the infiltrating population and distribution of Thy1.1 positive cells measured by flow cytometry at day eight p.i. Data represent means (±SD) of twelve mice per group combined from three separate experiments. (B) IL-17 mRNA expression determined by quantitative real-time PCR in infected SCID recipients of WT, WT/GKO or GKO CD4 + T cells. Data represent the mean of two experiments with n = 4 in each group per experiment. (C) Splenocytes of immunized GKO donors cultured in the presence of JHMV with or without recombinant IFN-γ (10 ng/ml) for six days and restimulated four hours with PMA/Ionomycin. Intracellular cytokine expression on CD4 + T cells analyzed by flow cytometry using FITC-IFN-γ, PE-IL-17 and the corresponding isotype controls. Dot plots are representative of duplicates from two separate experiments. (D) Survival of infected SCID recipients of WT/GKO (n = 6), WT/GKO + anti-IFN-γ mAb (n = 8) and GKO (n = 4) CD4 + T cells assessed daily. Data are representative of two separate experiments.

    Journal: Journal of Neuroinflammation

    Article Title: IFN-? protects from lethal IL-17 mediated viral encephalomyelitis independent of neutrophils

    doi: 10.1186/1742-2094-9-104

    Figure Lengend Snippet: IFN-γ-mediated protection prevents IL-17-mediated mortality. (A) Number of CD4 + T cells in the infiltrating population and distribution of Thy1.1 positive cells measured by flow cytometry at day eight p.i. Data represent means (±SD) of twelve mice per group combined from three separate experiments. (B) IL-17 mRNA expression determined by quantitative real-time PCR in infected SCID recipients of WT, WT/GKO or GKO CD4 + T cells. Data represent the mean of two experiments with n = 4 in each group per experiment. (C) Splenocytes of immunized GKO donors cultured in the presence of JHMV with or without recombinant IFN-γ (10 ng/ml) for six days and restimulated four hours with PMA/Ionomycin. Intracellular cytokine expression on CD4 + T cells analyzed by flow cytometry using FITC-IFN-γ, PE-IL-17 and the corresponding isotype controls. Dot plots are representative of duplicates from two separate experiments. (D) Survival of infected SCID recipients of WT/GKO (n = 6), WT/GKO + anti-IFN-γ mAb (n = 8) and GKO (n = 4) CD4 + T cells assessed daily. Data are representative of two separate experiments.

    Article Snippet: Cytokine production from both splenic cultures or ex vivo lymph node cells was measured following four hours stimulation with PMA (10 ng/ml) (Acros Organics, Geel, Belgium) and ionomycin (1 μM) (Calbiotech, Spring Valley, CA, USA).

    Techniques: Flow Cytometry, Cytometry, Mouse Assay, Expressing, Real-time Polymerase Chain Reaction, Infection, Cell Culture, Recombinant