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Boehringer Mannheim ionomycin
Pertussis toxin (PT) does not influence HIV LTR activity in Jurkat cells. Jurkat cells co-transfected with pLTR-chloramphenicol acetyl transferase (CAT) and pSVtat were cultured in RPMI or RPMI containing PT for 24 h. The cells were split into two treatment groups receiving either no treatment or phorbol myristate acetate (PMA) and <t>ionomycin</t> (PI). Following 18 h of culture CAT levels in cell lysates were measured. Results show the mean and s.d. of three independent experiments.
Ionomycin, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ionomycin/product/Boehringer Mannheim
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Images

1) Product Images from "CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive"

Article Title: CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive

Journal: Clinical and Experimental Immunology

doi: 10.1046/j.1365-2249.1999.00897.x

Pertussis toxin (PT) does not influence HIV LTR activity in Jurkat cells. Jurkat cells co-transfected with pLTR-chloramphenicol acetyl transferase (CAT) and pSVtat were cultured in RPMI or RPMI containing PT for 24 h. The cells were split into two treatment groups receiving either no treatment or phorbol myristate acetate (PMA) and ionomycin (PI). Following 18 h of culture CAT levels in cell lysates were measured. Results show the mean and s.d. of three independent experiments.
Figure Legend Snippet: Pertussis toxin (PT) does not influence HIV LTR activity in Jurkat cells. Jurkat cells co-transfected with pLTR-chloramphenicol acetyl transferase (CAT) and pSVtat were cultured in RPMI or RPMI containing PT for 24 h. The cells were split into two treatment groups receiving either no treatment or phorbol myristate acetate (PMA) and ionomycin (PI). Following 18 h of culture CAT levels in cell lysates were measured. Results show the mean and s.d. of three independent experiments.

Techniques Used: Activity Assay, Transfection, Chloramphenicol Acetyltransferase Assay, Cell Culture

(a) Effect of the G protein inhibitor pertussis toxin (PT) on CD8-mediated enhancement of LTR-mediated gene expression. U38 cells transfected with pSVtat were cultured with RPMI or with CD8 + T cell supernatant:RPMI (1:1) in the absence and presence of cholera toxin (CT), cyclosporin A (CsA) or PT for 18 h prior to stimulation with phorbol myristate acetate (PMA) and ionomycin. Chloramphenicol acetyl transferase (CAT) levels were measured in cell lysates following 18 h stimulation. Results show the mean and s.d. of four independent experiments. (b) U38 cells transfected with pSVtat were cultured in RPMI or RPMI containing either CsA or PT for 24 h and CAT levels were measured in cell lysates following 18 h stimulation with PMA and ionomycin. Results show the mean and s.d. of four independent experiments.
Figure Legend Snippet: (a) Effect of the G protein inhibitor pertussis toxin (PT) on CD8-mediated enhancement of LTR-mediated gene expression. U38 cells transfected with pSVtat were cultured with RPMI or with CD8 + T cell supernatant:RPMI (1:1) in the absence and presence of cholera toxin (CT), cyclosporin A (CsA) or PT for 18 h prior to stimulation with phorbol myristate acetate (PMA) and ionomycin. Chloramphenicol acetyl transferase (CAT) levels were measured in cell lysates following 18 h stimulation. Results show the mean and s.d. of four independent experiments. (b) U38 cells transfected with pSVtat were cultured in RPMI or RPMI containing either CsA or PT for 24 h and CAT levels were measured in cell lysates following 18 h stimulation with PMA and ionomycin. Results show the mean and s.d. of four independent experiments.

Techniques Used: Expressing, Transfection, Cell Culture, Chloramphenicol Acetyltransferase Assay

Pertussis toxin (PT) sensitivity of CD8-mediated enhancement of transcription is dose-dependent. U38 cells were transfected with pSVtat and cultured with RPMI in the presence and absence of CD8 + T cell supernatant. PT was included at the indicated doses. Cells were lysed for measurement of chloramphenicol acetyl transferase (CAT) 18 h following treatment with phorbol myristate acetate (PMA) and ionomycin. Results show the mean and s.d. of three independent experiments.
Figure Legend Snippet: Pertussis toxin (PT) sensitivity of CD8-mediated enhancement of transcription is dose-dependent. U38 cells were transfected with pSVtat and cultured with RPMI in the presence and absence of CD8 + T cell supernatant. PT was included at the indicated doses. Cells were lysed for measurement of chloramphenicol acetyl transferase (CAT) 18 h following treatment with phorbol myristate acetate (PMA) and ionomycin. Results show the mean and s.d. of three independent experiments.

Techniques Used: Transfection, Cell Culture, Chloramphenicol Acetyltransferase Assay

2) Product Images from "Mechanism Involved in Initiation and Propagation of Receptor-Induced Intercellular Calcium Signaling in Cultured Rat Astrocytes"

Article Title: Mechanism Involved in Initiation and Propagation of Receptor-Induced Intercellular Calcium Signaling in Cultured Rat Astrocytes

Journal: The Journal of Neuroscience

doi: 10.1523/JNEUROSCI.17-06-01981.1997

Quantification of amplitude of intercellular calcium signals and extent in cultured astrocytes. Analysis of intercellular calcium signaling generated ( A ) by mechanical stimulation ( n = 12) and ( B ) by focal application of ionomycin ( n = 21). Relative amplitude of [Ca 2+ ] i increases ( left scales , open columns ) and number of unresponsive cells ( right scales , dashed line ) were plotted by identifying the cellular row from which they were recorded. Cells were classified according to their location in reference to the stimulated cell, numbered 0 . Typically, stimulation was performed in the center of a microscopic field composed of ∼30 cells forming approximately six to seven cellular rows. As the distance from the stimulated cell increased, the amplitude of the response decreased, and more cells did not respond. For both modes of stimulation, the extent of intercellular calcium signaling generally exceeded the investigated cell population.
Figure Legend Snippet: Quantification of amplitude of intercellular calcium signals and extent in cultured astrocytes. Analysis of intercellular calcium signaling generated ( A ) by mechanical stimulation ( n = 12) and ( B ) by focal application of ionomycin ( n = 21). Relative amplitude of [Ca 2+ ] i increases ( left scales , open columns ) and number of unresponsive cells ( right scales , dashed line ) were plotted by identifying the cellular row from which they were recorded. Cells were classified according to their location in reference to the stimulated cell, numbered 0 . Typically, stimulation was performed in the center of a microscopic field composed of ∼30 cells forming approximately six to seven cellular rows. As the distance from the stimulated cell increased, the amplitude of the response decreased, and more cells did not respond. For both modes of stimulation, the extent of intercellular calcium signaling generally exceeded the investigated cell population.

Techniques Used: Cell Culture, Generated

3) Product Images from "Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells"

Article Title: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

Journal: Stem Cell Research & Therapy

doi: 10.1186/scrt537

Percentage of CD4 + and CD8 + T cells expressing interleukin-17 (IL-17). The left upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells producing IL-17 (within total CD4 + T cells and total CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The right upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells producing simultaneously IL-17 and tumor necrosis factor-alpha (TNF-α) (within IL-17 + CD4 + T cells and IL-17 + CD8 + T cells, respectively) and amount of protein expressed per cell, measured as MFI (MFI, mean ± standard deviation), after MNC stimulation with PMA plus ionomycin in the absence of MSCs (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IL-17 or IL-17 and TNF-α. Statistically significant differences were considered when P
Figure Legend Snippet: Percentage of CD4 + and CD8 + T cells expressing interleukin-17 (IL-17). The left upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells producing IL-17 (within total CD4 + T cells and total CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The right upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells producing simultaneously IL-17 and tumor necrosis factor-alpha (TNF-α) (within IL-17 + CD4 + T cells and IL-17 + CD8 + T cells, respectively) and amount of protein expressed per cell, measured as MFI (MFI, mean ± standard deviation), after MNC stimulation with PMA plus ionomycin in the absence of MSCs (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IL-17 or IL-17 and TNF-α. Statistically significant differences were considered when P

Techniques Used: Expressing, Standard Deviation, Fluorescence, Co-Culture Assay, Inhibition

Percentage of CD4 + and CD8 + T cells expressing interferon gamma (IFNγ). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing IFNγ (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IFNγ. Statistically significant differences were considered when P
Figure Legend Snippet: Percentage of CD4 + and CD8 + T cells expressing interferon gamma (IFNγ). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing IFNγ (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IFNγ. Statistically significant differences were considered when P

Techniques Used: Expressing, Standard Deviation, Functional Assay, Fluorescence, Co-Culture Assay, Inhibition

mRNA expression of TNF-α, IL-8, IL-1β, TGF-β, ICOSL, and IDO in MSCs. Semi-quantitative analysis of TNF-α, IL-8, IL-1β, TGF-β, ICOSL, IDO mRNA expression in non-stimulated MSCs (MSCs) and in MSCs stimulated with PMA plus ionomycin (MSCs + PMA + ionomycin). Statistically significant differences were considered when P
Figure Legend Snippet: mRNA expression of TNF-α, IL-8, IL-1β, TGF-β, ICOSL, and IDO in MSCs. Semi-quantitative analysis of TNF-α, IL-8, IL-1β, TGF-β, ICOSL, IDO mRNA expression in non-stimulated MSCs (MSCs) and in MSCs stimulated with PMA plus ionomycin (MSCs + PMA + ionomycin). Statistically significant differences were considered when P

Techniques Used: Expressing

Percentage of CD4 + and CD8 + T cells expressing interleukin-2 (IL-2). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing IL-2 (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IL-2. Statistically significant differences were considered when P
Figure Legend Snippet: Percentage of CD4 + and CD8 + T cells expressing interleukin-2 (IL-2). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing IL-2 (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IL-2. Statistically significant differences were considered when P

Techniques Used: Expressing, Standard Deviation, Functional Assay, Fluorescence, Co-Culture Assay, Inhibition

Percentage of CD4 + and CD8 + T cells expressing interleukin-6 (IL-6) or IL-9. The upper panel shows the percentage (mean ± standard deviation) of CD4 + T cells expressing IL-6 (within total CD4 + T cells) and of total, CD4 + , and CD8 + T cells producing IL-9 (within total T cells, CD4 + T cells, and total CD8 + T cells, respectively), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel shows the percentage of inhibition induced by MSCs on the percentage of total, CD4 + and/or CD8 + T cells expressing IL-6 or IL-9. Statistically significant differences were considered when P
Figure Legend Snippet: Percentage of CD4 + and CD8 + T cells expressing interleukin-6 (IL-6) or IL-9. The upper panel shows the percentage (mean ± standard deviation) of CD4 + T cells expressing IL-6 (within total CD4 + T cells) and of total, CD4 + , and CD8 + T cells producing IL-9 (within total T cells, CD4 + T cells, and total CD8 + T cells, respectively), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel shows the percentage of inhibition induced by MSCs on the percentage of total, CD4 + and/or CD8 + T cells expressing IL-6 or IL-9. Statistically significant differences were considered when P

Techniques Used: Expressing, Standard Deviation, Co-Culture Assay, Inhibition

Percentage of CD4 + and CD8 + T cells expressing tumor necrosis factor-alpha (TNF-α). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing TNF-α (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing TNF-α. Statistically significant differences were considered when P
Figure Legend Snippet: Percentage of CD4 + and CD8 + T cells expressing tumor necrosis factor-alpha (TNF-α). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing TNF-α (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing TNF-α. Statistically significant differences were considered when P

Techniques Used: Expressing, Standard Deviation, Functional Assay, Fluorescence, Co-Culture Assay, Inhibition

mRNA expression of interleukin-10 (IL-10), transforming growth factor-beta (TGF-β), IL-2, IL-4, and CTLA-4 in CD4 + and CD8 + T cells. The figure shows the results of a semi-quantitative analysis of IL-10, TGF-β, IL-2, IL-4, and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) mRNA expression in fluorescence-activated cell sorting (FACS)-purified CD4 + and CD8 + T cells from the following culture conditions: non-stimulated mononuclear cells (MNCs), non-stimulated MNCs co-cultured with mesenchymal stromal cells (MSCs) (MNCs + MSCs), MNCs stimulated with phorbol myristate acetate (PMA) plus ionomycin (MNCs + PMA + ionomycin), and MNCs stimulated with PMA plus ionomycin in co-culture with MSCs (MNCs + MSCs + PMA + ionomycin). Statistically significant differences were considered when P
Figure Legend Snippet: mRNA expression of interleukin-10 (IL-10), transforming growth factor-beta (TGF-β), IL-2, IL-4, and CTLA-4 in CD4 + and CD8 + T cells. The figure shows the results of a semi-quantitative analysis of IL-10, TGF-β, IL-2, IL-4, and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) mRNA expression in fluorescence-activated cell sorting (FACS)-purified CD4 + and CD8 + T cells from the following culture conditions: non-stimulated mononuclear cells (MNCs), non-stimulated MNCs co-cultured with mesenchymal stromal cells (MSCs) (MNCs + MSCs), MNCs stimulated with phorbol myristate acetate (PMA) plus ionomycin (MNCs + PMA + ionomycin), and MNCs stimulated with PMA plus ionomycin in co-culture with MSCs (MNCs + MSCs + PMA + ionomycin). Statistically significant differences were considered when P

Techniques Used: Expressing, Fluorescence, FACS, Purification, Cell Culture, Co-Culture Assay

Related Articles

Enzyme-linked Immunosorbent Assay:

Article Title: CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive
Article Snippet: .. Twenty-four hours following transfection the cells were treated with PMA and ionomycin or TNF-α for 18 h. The cells were then lysed by four rounds of freeze/thaw and the lysate supernatant was used to measure CAT activity using a CAT ELISA system (Boehringer Mannheim, Laval, Quebec, Canada). .. U1 cells were transfected with 10 μg pLTRCAT and cultured with CD8+ T cell supernatant in the absence and presence of PT.

Transfection:

Article Title: CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive
Article Snippet: .. Twenty-four hours following transfection the cells were treated with PMA and ionomycin or TNF-α for 18 h. The cells were then lysed by four rounds of freeze/thaw and the lysate supernatant was used to measure CAT activity using a CAT ELISA system (Boehringer Mannheim, Laval, Quebec, Canada). .. U1 cells were transfected with 10 μg pLTRCAT and cultured with CD8+ T cell supernatant in the absence and presence of PT.

other:

Article Title: Biosynthesis of an Endogenous Cannabinoid Precursor in Neurons and its Control by Calcium and cAMP
Article Snippet: NAPE (1-palmitoyl,2-oleoyl- sn -glycerol-3-phosphoethanolamine- N -arachidonoyl), PE, PC, sphingomyelin, and cerebrosides were obtained from Avanti Polar Lipids (Alabaster, AL); diacylglycerols, triacylglycerols, monoacylglycerols, cholesterol, and free fatty acids were from Nu-Chek Prep (Elysian, MN); calmidazolium, chelerythrine, 1,8-dideoxyforskolin, forskolin, KN-62, and 4-β-phorbol-myristate-acetate were from Research Biochemicals (Natick, MA); ionomycin was from Boehringer Mannheim (Indianapolis, IN), and vasoactive intestinal peptide was from Calbiochem (La Jolla, CA); [3 H]ethanolamine, [3 H]arachidonic acid, [14 C]arachidonic acid, and [3 H]palmitic acid were from Amersham (Arlington Heights, IL); [3 H]oleic acid and [14 C]oleoyl-coenzyme A were from American Radiolabeled Products (St. Louis, MO); all other chemicals were from Sigma (St. Louis, MO).

Activity Assay:

Article Title: CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive
Article Snippet: .. Twenty-four hours following transfection the cells were treated with PMA and ionomycin or TNF-α for 18 h. The cells were then lysed by four rounds of freeze/thaw and the lysate supernatant was used to measure CAT activity using a CAT ELISA system (Boehringer Mannheim, Laval, Quebec, Canada). .. U1 cells were transfected with 10 μg pLTRCAT and cultured with CD8+ T cell supernatant in the absence and presence of PT.

Chloramphenicol Acetyltransferase Assay:

Article Title: CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive
Article Snippet: .. Twenty-four hours following transfection the cells were treated with PMA and ionomycin or TNF-α for 18 h. The cells were then lysed by four rounds of freeze/thaw and the lysate supernatant was used to measure CAT activity using a CAT ELISA system (Boehringer Mannheim, Laval, Quebec, Canada). .. U1 cells were transfected with 10 μg pLTRCAT and cultured with CD8+ T cell supernatant in the absence and presence of PT.

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    Boehringer Mannheim ionomycin
    Pertussis toxin (PT) does not influence HIV LTR activity in Jurkat cells. Jurkat cells co-transfected with pLTR-chloramphenicol acetyl transferase (CAT) and pSVtat were cultured in RPMI or RPMI containing PT for 24 h. The cells were split into two treatment groups receiving either no treatment or phorbol myristate acetate (PMA) and <t>ionomycin</t> (PI). Following 18 h of culture CAT levels in cell lysates were measured. Results show the mean and s.d. of three independent experiments.
    Ionomycin, supplied by Boehringer Mannheim, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ionomycin/product/Boehringer Mannheim
    Average 93 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    ionomycin - by Bioz Stars, 2020-09
    93/100 stars
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    Pertussis toxin (PT) does not influence HIV LTR activity in Jurkat cells. Jurkat cells co-transfected with pLTR-chloramphenicol acetyl transferase (CAT) and pSVtat were cultured in RPMI or RPMI containing PT for 24 h. The cells were split into two treatment groups receiving either no treatment or phorbol myristate acetate (PMA) and ionomycin (PI). Following 18 h of culture CAT levels in cell lysates were measured. Results show the mean and s.d. of three independent experiments.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive

    doi: 10.1046/j.1365-2249.1999.00897.x

    Figure Lengend Snippet: Pertussis toxin (PT) does not influence HIV LTR activity in Jurkat cells. Jurkat cells co-transfected with pLTR-chloramphenicol acetyl transferase (CAT) and pSVtat were cultured in RPMI or RPMI containing PT for 24 h. The cells were split into two treatment groups receiving either no treatment or phorbol myristate acetate (PMA) and ionomycin (PI). Following 18 h of culture CAT levels in cell lysates were measured. Results show the mean and s.d. of three independent experiments.

    Article Snippet: Twenty-four hours following transfection the cells were treated with PMA and ionomycin or TNF-α for 18 h. The cells were then lysed by four rounds of freeze/thaw and the lysate supernatant was used to measure CAT activity using a CAT ELISA system (Boehringer Mannheim, Laval, Quebec, Canada).

    Techniques: Activity Assay, Transfection, Chloramphenicol Acetyltransferase Assay, Cell Culture

    (a) Effect of the G protein inhibitor pertussis toxin (PT) on CD8-mediated enhancement of LTR-mediated gene expression. U38 cells transfected with pSVtat were cultured with RPMI or with CD8 + T cell supernatant:RPMI (1:1) in the absence and presence of cholera toxin (CT), cyclosporin A (CsA) or PT for 18 h prior to stimulation with phorbol myristate acetate (PMA) and ionomycin. Chloramphenicol acetyl transferase (CAT) levels were measured in cell lysates following 18 h stimulation. Results show the mean and s.d. of four independent experiments. (b) U38 cells transfected with pSVtat were cultured in RPMI or RPMI containing either CsA or PT for 24 h and CAT levels were measured in cell lysates following 18 h stimulation with PMA and ionomycin. Results show the mean and s.d. of four independent experiments.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive

    doi: 10.1046/j.1365-2249.1999.00897.x

    Figure Lengend Snippet: (a) Effect of the G protein inhibitor pertussis toxin (PT) on CD8-mediated enhancement of LTR-mediated gene expression. U38 cells transfected with pSVtat were cultured with RPMI or with CD8 + T cell supernatant:RPMI (1:1) in the absence and presence of cholera toxin (CT), cyclosporin A (CsA) or PT for 18 h prior to stimulation with phorbol myristate acetate (PMA) and ionomycin. Chloramphenicol acetyl transferase (CAT) levels were measured in cell lysates following 18 h stimulation. Results show the mean and s.d. of four independent experiments. (b) U38 cells transfected with pSVtat were cultured in RPMI or RPMI containing either CsA or PT for 24 h and CAT levels were measured in cell lysates following 18 h stimulation with PMA and ionomycin. Results show the mean and s.d. of four independent experiments.

    Article Snippet: Twenty-four hours following transfection the cells were treated with PMA and ionomycin or TNF-α for 18 h. The cells were then lysed by four rounds of freeze/thaw and the lysate supernatant was used to measure CAT activity using a CAT ELISA system (Boehringer Mannheim, Laval, Quebec, Canada).

    Techniques: Expressing, Transfection, Cell Culture, Chloramphenicol Acetyltransferase Assay

    Pertussis toxin (PT) sensitivity of CD8-mediated enhancement of transcription is dose-dependent. U38 cells were transfected with pSVtat and cultured with RPMI in the presence and absence of CD8 + T cell supernatant. PT was included at the indicated doses. Cells were lysed for measurement of chloramphenicol acetyl transferase (CAT) 18 h following treatment with phorbol myristate acetate (PMA) and ionomycin. Results show the mean and s.d. of three independent experiments.

    Journal: Clinical and Experimental Immunology

    Article Title: CD8+T cell-mediated enhancement of tumour necrosis factor-alpha (TNF-?) production and HIV-1 LTR-driven gene expression in human monocytic cells is pertussis toxin-sensitive

    doi: 10.1046/j.1365-2249.1999.00897.x

    Figure Lengend Snippet: Pertussis toxin (PT) sensitivity of CD8-mediated enhancement of transcription is dose-dependent. U38 cells were transfected with pSVtat and cultured with RPMI in the presence and absence of CD8 + T cell supernatant. PT was included at the indicated doses. Cells were lysed for measurement of chloramphenicol acetyl transferase (CAT) 18 h following treatment with phorbol myristate acetate (PMA) and ionomycin. Results show the mean and s.d. of three independent experiments.

    Article Snippet: Twenty-four hours following transfection the cells were treated with PMA and ionomycin or TNF-α for 18 h. The cells were then lysed by four rounds of freeze/thaw and the lysate supernatant was used to measure CAT activity using a CAT ELISA system (Boehringer Mannheim, Laval, Quebec, Canada).

    Techniques: Transfection, Cell Culture, Chloramphenicol Acetyltransferase Assay

    Quantification of amplitude of intercellular calcium signals and extent in cultured astrocytes. Analysis of intercellular calcium signaling generated ( A ) by mechanical stimulation ( n = 12) and ( B ) by focal application of ionomycin ( n = 21). Relative amplitude of [Ca 2+ ] i increases ( left scales , open columns ) and number of unresponsive cells ( right scales , dashed line ) were plotted by identifying the cellular row from which they were recorded. Cells were classified according to their location in reference to the stimulated cell, numbered 0 . Typically, stimulation was performed in the center of a microscopic field composed of ∼30 cells forming approximately six to seven cellular rows. As the distance from the stimulated cell increased, the amplitude of the response decreased, and more cells did not respond. For both modes of stimulation, the extent of intercellular calcium signaling generally exceeded the investigated cell population.

    Journal: The Journal of Neuroscience

    Article Title: Mechanism Involved in Initiation and Propagation of Receptor-Induced Intercellular Calcium Signaling in Cultured Rat Astrocytes

    doi: 10.1523/JNEUROSCI.17-06-01981.1997

    Figure Lengend Snippet: Quantification of amplitude of intercellular calcium signals and extent in cultured astrocytes. Analysis of intercellular calcium signaling generated ( A ) by mechanical stimulation ( n = 12) and ( B ) by focal application of ionomycin ( n = 21). Relative amplitude of [Ca 2+ ] i increases ( left scales , open columns ) and number of unresponsive cells ( right scales , dashed line ) were plotted by identifying the cellular row from which they were recorded. Cells were classified according to their location in reference to the stimulated cell, numbered 0 . Typically, stimulation was performed in the center of a microscopic field composed of ∼30 cells forming approximately six to seven cellular rows. As the distance from the stimulated cell increased, the amplitude of the response decreased, and more cells did not respond. For both modes of stimulation, the extent of intercellular calcium signaling generally exceeded the investigated cell population.

    Article Snippet: All drugs used were purchased from Sigma, except for U-73122 and U-73343 (Biomol, Plymouth, PA), ionomycin (Boehringer Mannheim, Mannheim, Germany), myo-[2-3 H]inositol (Amersham, Les Ulis, France), l (+)-2-amino-3-phosphonopropionic acid (L-AP3 ) and 6,7-dinitroquinoxaline-2,3-dione (DNQX) (Tocris Cookson, Bristol, UK), and endothelin-1 (Neosystem, Strasbourg, France).

    Techniques: Cell Culture, Generated

    Percentage of CD4 + and CD8 + T cells expressing interleukin-17 (IL-17). The left upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells producing IL-17 (within total CD4 + T cells and total CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The right upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells producing simultaneously IL-17 and tumor necrosis factor-alpha (TNF-α) (within IL-17 + CD4 + T cells and IL-17 + CD8 + T cells, respectively) and amount of protein expressed per cell, measured as MFI (MFI, mean ± standard deviation), after MNC stimulation with PMA plus ionomycin in the absence of MSCs (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IL-17 or IL-17 and TNF-α. Statistically significant differences were considered when P

    Journal: Stem Cell Research & Therapy

    Article Title: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

    doi: 10.1186/scrt537

    Figure Lengend Snippet: Percentage of CD4 + and CD8 + T cells expressing interleukin-17 (IL-17). The left upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells producing IL-17 (within total CD4 + T cells and total CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The right upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells producing simultaneously IL-17 and tumor necrosis factor-alpha (TNF-α) (within IL-17 + CD4 + T cells and IL-17 + CD8 + T cells, respectively) and amount of protein expressed per cell, measured as MFI (MFI, mean ± standard deviation), after MNC stimulation with PMA plus ionomycin in the absence of MSCs (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IL-17 or IL-17 and TNF-α. Statistically significant differences were considered when P

    Article Snippet: MSCs from two of the four wells were stimulated with PMA (50 ng/mL; Sigma-Aldrich) plus ionomycin (1 μg/mL; Boehringer Mannheim, Germany) for 4 hours at 37°C in humidified atmosphere containing 5% CO2 ; in the remaining two wells, MSCs were not stimulated.

    Techniques: Expressing, Standard Deviation, Fluorescence, Co-Culture Assay, Inhibition

    Percentage of CD4 + and CD8 + T cells expressing interferon gamma (IFNγ). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing IFNγ (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IFNγ. Statistically significant differences were considered when P

    Journal: Stem Cell Research & Therapy

    Article Title: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

    doi: 10.1186/scrt537

    Figure Lengend Snippet: Percentage of CD4 + and CD8 + T cells expressing interferon gamma (IFNγ). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing IFNγ (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IFNγ. Statistically significant differences were considered when P

    Article Snippet: MSCs from two of the four wells were stimulated with PMA (50 ng/mL; Sigma-Aldrich) plus ionomycin (1 μg/mL; Boehringer Mannheim, Germany) for 4 hours at 37°C in humidified atmosphere containing 5% CO2 ; in the remaining two wells, MSCs were not stimulated.

    Techniques: Expressing, Standard Deviation, Functional Assay, Fluorescence, Co-Culture Assay, Inhibition

    mRNA expression of TNF-α, IL-8, IL-1β, TGF-β, ICOSL, and IDO in MSCs. Semi-quantitative analysis of TNF-α, IL-8, IL-1β, TGF-β, ICOSL, IDO mRNA expression in non-stimulated MSCs (MSCs) and in MSCs stimulated with PMA plus ionomycin (MSCs + PMA + ionomycin). Statistically significant differences were considered when P

    Journal: Stem Cell Research & Therapy

    Article Title: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

    doi: 10.1186/scrt537

    Figure Lengend Snippet: mRNA expression of TNF-α, IL-8, IL-1β, TGF-β, ICOSL, and IDO in MSCs. Semi-quantitative analysis of TNF-α, IL-8, IL-1β, TGF-β, ICOSL, IDO mRNA expression in non-stimulated MSCs (MSCs) and in MSCs stimulated with PMA plus ionomycin (MSCs + PMA + ionomycin). Statistically significant differences were considered when P

    Article Snippet: MSCs from two of the four wells were stimulated with PMA (50 ng/mL; Sigma-Aldrich) plus ionomycin (1 μg/mL; Boehringer Mannheim, Germany) for 4 hours at 37°C in humidified atmosphere containing 5% CO2 ; in the remaining two wells, MSCs were not stimulated.

    Techniques: Expressing

    Percentage of CD4 + and CD8 + T cells expressing interleukin-2 (IL-2). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing IL-2 (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IL-2. Statistically significant differences were considered when P

    Journal: Stem Cell Research & Therapy

    Article Title: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

    doi: 10.1186/scrt537

    Figure Lengend Snippet: Percentage of CD4 + and CD8 + T cells expressing interleukin-2 (IL-2). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing IL-2 (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing IL-2. Statistically significant differences were considered when P

    Article Snippet: MSCs from two of the four wells were stimulated with PMA (50 ng/mL; Sigma-Aldrich) plus ionomycin (1 μg/mL; Boehringer Mannheim, Germany) for 4 hours at 37°C in humidified atmosphere containing 5% CO2 ; in the remaining two wells, MSCs were not stimulated.

    Techniques: Expressing, Standard Deviation, Functional Assay, Fluorescence, Co-Culture Assay, Inhibition

    Percentage of CD4 + and CD8 + T cells expressing interleukin-6 (IL-6) or IL-9. The upper panel shows the percentage (mean ± standard deviation) of CD4 + T cells expressing IL-6 (within total CD4 + T cells) and of total, CD4 + , and CD8 + T cells producing IL-9 (within total T cells, CD4 + T cells, and total CD8 + T cells, respectively), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel shows the percentage of inhibition induced by MSCs on the percentage of total, CD4 + and/or CD8 + T cells expressing IL-6 or IL-9. Statistically significant differences were considered when P

    Journal: Stem Cell Research & Therapy

    Article Title: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

    doi: 10.1186/scrt537

    Figure Lengend Snippet: Percentage of CD4 + and CD8 + T cells expressing interleukin-6 (IL-6) or IL-9. The upper panel shows the percentage (mean ± standard deviation) of CD4 + T cells expressing IL-6 (within total CD4 + T cells) and of total, CD4 + , and CD8 + T cells producing IL-9 (within total T cells, CD4 + T cells, and total CD8 + T cells, respectively), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel shows the percentage of inhibition induced by MSCs on the percentage of total, CD4 + and/or CD8 + T cells expressing IL-6 or IL-9. Statistically significant differences were considered when P

    Article Snippet: MSCs from two of the four wells were stimulated with PMA (50 ng/mL; Sigma-Aldrich) plus ionomycin (1 μg/mL; Boehringer Mannheim, Germany) for 4 hours at 37°C in humidified atmosphere containing 5% CO2 ; in the remaining two wells, MSCs were not stimulated.

    Techniques: Expressing, Standard Deviation, Co-Culture Assay, Inhibition

    Percentage of CD4 + and CD8 + T cells expressing tumor necrosis factor-alpha (TNF-α). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing TNF-α (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing TNF-α. Statistically significant differences were considered when P

    Journal: Stem Cell Research & Therapy

    Article Title: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

    doi: 10.1186/scrt537

    Figure Lengend Snippet: Percentage of CD4 + and CD8 + T cells expressing tumor necrosis factor-alpha (TNF-α). The upper panels show the percentage (mean ± standard deviation) of CD4 + and CD8 + T cells, distributed among their functional compartments, producing TNF-α (within the correspondent functional compartment of CD4 + or CD8 + T cells, respectively) and amount of protein expressed per cell, measured as mean fluorescence intensity (MFI) (mean ± standard deviation), after mononuclear cell (MNC) stimulation with phorbol myristate acetate (PMA) plus ionomycin in the absence of mesenchymal stromal cells (MSCs) (MNCs + PMA + ionomycin) or in co-culture with MSCs (MSCs + MNCs + PMA + ionomycin). The lower panel represents the percentage of inhibition induced by MSCs on the percentage of CD4 + and CD8 + T cells expressing TNF-α. Statistically significant differences were considered when P

    Article Snippet: MSCs from two of the four wells were stimulated with PMA (50 ng/mL; Sigma-Aldrich) plus ionomycin (1 μg/mL; Boehringer Mannheim, Germany) for 4 hours at 37°C in humidified atmosphere containing 5% CO2 ; in the remaining two wells, MSCs were not stimulated.

    Techniques: Expressing, Standard Deviation, Functional Assay, Fluorescence, Co-Culture Assay, Inhibition

    mRNA expression of interleukin-10 (IL-10), transforming growth factor-beta (TGF-β), IL-2, IL-4, and CTLA-4 in CD4 + and CD8 + T cells. The figure shows the results of a semi-quantitative analysis of IL-10, TGF-β, IL-2, IL-4, and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) mRNA expression in fluorescence-activated cell sorting (FACS)-purified CD4 + and CD8 + T cells from the following culture conditions: non-stimulated mononuclear cells (MNCs), non-stimulated MNCs co-cultured with mesenchymal stromal cells (MSCs) (MNCs + MSCs), MNCs stimulated with phorbol myristate acetate (PMA) plus ionomycin (MNCs + PMA + ionomycin), and MNCs stimulated with PMA plus ionomycin in co-culture with MSCs (MNCs + MSCs + PMA + ionomycin). Statistically significant differences were considered when P

    Journal: Stem Cell Research & Therapy

    Article Title: Effect of human bone marrow mesenchymal stromal cells on cytokine production by peripheral blood naive, memory, and effector T cells

    doi: 10.1186/scrt537

    Figure Lengend Snippet: mRNA expression of interleukin-10 (IL-10), transforming growth factor-beta (TGF-β), IL-2, IL-4, and CTLA-4 in CD4 + and CD8 + T cells. The figure shows the results of a semi-quantitative analysis of IL-10, TGF-β, IL-2, IL-4, and CTLA-4 (cytotoxic T-lymphocyte-associated protein 4) mRNA expression in fluorescence-activated cell sorting (FACS)-purified CD4 + and CD8 + T cells from the following culture conditions: non-stimulated mononuclear cells (MNCs), non-stimulated MNCs co-cultured with mesenchymal stromal cells (MSCs) (MNCs + MSCs), MNCs stimulated with phorbol myristate acetate (PMA) plus ionomycin (MNCs + PMA + ionomycin), and MNCs stimulated with PMA plus ionomycin in co-culture with MSCs (MNCs + MSCs + PMA + ionomycin). Statistically significant differences were considered when P

    Article Snippet: MSCs from two of the four wells were stimulated with PMA (50 ng/mL; Sigma-Aldrich) plus ionomycin (1 μg/mL; Boehringer Mannheim, Germany) for 4 hours at 37°C in humidified atmosphere containing 5% CO2 ; in the remaining two wells, MSCs were not stimulated.

    Techniques: Expressing, Fluorescence, FACS, Purification, Cell Culture, Co-Culture Assay