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Biogems International ionomycin
Ionomycin, supplied by Biogems International, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ionomycin/product/Biogems International
Average 93 stars, based on 2 article reviews
Price from $9.99 to $1999.99
ionomycin - by Bioz Stars, 2020-09
93/100 stars

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Article Title: Distribution and functions of γδ T cells infiltrated in the ovarian cancer microenvironment
Article Snippet: For intracellular cytokine detection, PBMCs or TILs (1 × 106 ) were resuspended in RPMI 1640 medium supplemented with 10% FBS (Gibco) and stimulated with phorbol-12-myristate 13-acetate (50 ng/ml), ionomycin (1 μg/ml), and brefeldin (1 μg/ml) (all from Biogems, Rocky Hill, NJ, USA) for 5 h in 5% CO2 atmosphere at 37 °C.

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    Biogems International ionomycin calcium salt
    Reversal of acidic microenvironment restores Foxp3 expression and iTreg function. (A) The proportion of CD4 + CD25 + Foxp3 + iTregs cultured with IL-2 and TGF-β for 3 days. (B,C) The proportion of CD4 + IL-10 + and CD4 + TGF-β + T cells cultured with IL-2 and TGF-β for 3 days and stimulated with phorbol 12-myristate 13-acetate, brefeldin A, and <t>ionomycin</t> for 6 h before the assay. (D) Statistical analysis of the suppression assay in vitro . (E) iTregs induced in media of varying pH values for 3 days were co-cultured with carboxyfluorescein succinimidyl ester-stained human naïve CD4 + T cells (responders) at the indicated ratio. After 72 h of activation with anti-CD3/CD28-conjugated beads, responder cell proliferation was assessed by flow cytometry. (F–H) Statistical analysis of related results of flow cytometry. Data are presented as the means ± SD from three independent experiments. *** p
    Ionomycin Calcium Salt, supplied by Biogems International, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ionomycin calcium salt/product/Biogems International
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ionomycin calcium salt - by Bioz Stars, 2020-09
    94/100 stars
      Buy from Supplier

    93
    Biogems International ionomycin
    Reversal of acidic microenvironment restores Foxp3 expression and iTreg function. (A) The proportion of CD4 + CD25 + Foxp3 + iTregs cultured with IL-2 and TGF-β for 3 days. (B,C) The proportion of CD4 + IL-10 + and CD4 + TGF-β + T cells cultured with IL-2 and TGF-β for 3 days and stimulated with phorbol 12-myristate 13-acetate, brefeldin A, and <t>ionomycin</t> for 6 h before the assay. (D) Statistical analysis of the suppression assay in vitro . (E) iTregs induced in media of varying pH values for 3 days were co-cultured with carboxyfluorescein succinimidyl ester-stained human naïve CD4 + T cells (responders) at the indicated ratio. After 72 h of activation with anti-CD3/CD28-conjugated beads, responder cell proliferation was assessed by flow cytometry. (F–H) Statistical analysis of related results of flow cytometry. Data are presented as the means ± SD from three independent experiments. *** p
    Ionomycin, supplied by Biogems International, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ionomycin/product/Biogems International
    Average 93 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    ionomycin - by Bioz Stars, 2020-09
    93/100 stars
      Buy from Supplier

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    Reversal of acidic microenvironment restores Foxp3 expression and iTreg function. (A) The proportion of CD4 + CD25 + Foxp3 + iTregs cultured with IL-2 and TGF-β for 3 days. (B,C) The proportion of CD4 + IL-10 + and CD4 + TGF-β + T cells cultured with IL-2 and TGF-β for 3 days and stimulated with phorbol 12-myristate 13-acetate, brefeldin A, and ionomycin for 6 h before the assay. (D) Statistical analysis of the suppression assay in vitro . (E) iTregs induced in media of varying pH values for 3 days were co-cultured with carboxyfluorescein succinimidyl ester-stained human naïve CD4 + T cells (responders) at the indicated ratio. After 72 h of activation with anti-CD3/CD28-conjugated beads, responder cell proliferation was assessed by flow cytometry. (F–H) Statistical analysis of related results of flow cytometry. Data are presented as the means ± SD from three independent experiments. *** p

    Journal: Frontiers in Immunology

    Article Title: Acidic Microenvironment Regulates the Severity of Hepatic Ischemia/Reperfusion Injury by Modulating the Generation and Function of Tregs via the PI3K-mTOR Pathway

    doi: 10.3389/fimmu.2019.02945

    Figure Lengend Snippet: Reversal of acidic microenvironment restores Foxp3 expression and iTreg function. (A) The proportion of CD4 + CD25 + Foxp3 + iTregs cultured with IL-2 and TGF-β for 3 days. (B,C) The proportion of CD4 + IL-10 + and CD4 + TGF-β + T cells cultured with IL-2 and TGF-β for 3 days and stimulated with phorbol 12-myristate 13-acetate, brefeldin A, and ionomycin for 6 h before the assay. (D) Statistical analysis of the suppression assay in vitro . (E) iTregs induced in media of varying pH values for 3 days were co-cultured with carboxyfluorescein succinimidyl ester-stained human naïve CD4 + T cells (responders) at the indicated ratio. After 72 h of activation with anti-CD3/CD28-conjugated beads, responder cell proliferation was assessed by flow cytometry. (F–H) Statistical analysis of related results of flow cytometry. Data are presented as the means ± SD from three independent experiments. *** p

    Article Snippet: For intracellular staining of cytokines, cells were stimulated with phorbol 12-myristate 13-acetate (50 ng/ml, Biogems), ionomycin calcium salt (1 μg/ml, Biogems), and brefeldin A (5 μg/ml, Biogems) for 6 h. Then, cells were stained with surface markers and further fixed/permeabilized (BioLegend) and stained for intracellular protein.

    Techniques: Expressing, Cell Culture, Suppression Assay, In Vitro, Staining, Activation Assay, Flow Cytometry