Structured Review

Avantor ionomycin
1-MT induces a Th2 function of DC stimulated with LPS. (A) Kinetic of secretion of IFNγ, IL-5 and IL-13 in MLR supernatants. MLR were conducted with control iDC (□), LPS-stimulated DC (○) and LPS-stimulated DC pre-treated with 1-MT (▲). Cytokines were measured in MLR supernatants at the indicated times. Mean ± SD of triplicates of one representative experiment out of three. (B) MLR were conducted for 5 days. After IL-2 expansion, T cells were stimulated with PMA and <t>ionomycin</t> in the presence of Brefeldin A and IL-5, IL-13 and IFNγ expression was analyzed by intracellular staining. Data are shown for 1/20 DC/T cell ratio and were similar for other ratios. Data of one representative experiment out of three.
Ionomycin, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ionomycin/product/Avantor
Average 92 stars, based on 2 article reviews
Price from $9.99 to $1999.99
ionomycin - by Bioz Stars, 2020-09
92/100 stars

Images

1) Product Images from "1-Methyl-tryptophan can interfere with TLR signaling in dendritic cells independently of IDO activity"

Article Title: 1-Methyl-tryptophan can interfere with TLR signaling in dendritic cells independently of IDO activity

Journal: Journal of immunology (Baltimore, Md. : 1950)

doi:

1-MT induces a Th2 function of DC stimulated with LPS. (A) Kinetic of secretion of IFNγ, IL-5 and IL-13 in MLR supernatants. MLR were conducted with control iDC (□), LPS-stimulated DC (○) and LPS-stimulated DC pre-treated with 1-MT (▲). Cytokines were measured in MLR supernatants at the indicated times. Mean ± SD of triplicates of one representative experiment out of three. (B) MLR were conducted for 5 days. After IL-2 expansion, T cells were stimulated with PMA and ionomycin in the presence of Brefeldin A and IL-5, IL-13 and IFNγ expression was analyzed by intracellular staining. Data are shown for 1/20 DC/T cell ratio and were similar for other ratios. Data of one representative experiment out of three.
Figure Legend Snippet: 1-MT induces a Th2 function of DC stimulated with LPS. (A) Kinetic of secretion of IFNγ, IL-5 and IL-13 in MLR supernatants. MLR were conducted with control iDC (□), LPS-stimulated DC (○) and LPS-stimulated DC pre-treated with 1-MT (▲). Cytokines were measured in MLR supernatants at the indicated times. Mean ± SD of triplicates of one representative experiment out of three. (B) MLR were conducted for 5 days. After IL-2 expansion, T cells were stimulated with PMA and ionomycin in the presence of Brefeldin A and IL-5, IL-13 and IFNγ expression was analyzed by intracellular staining. Data are shown for 1/20 DC/T cell ratio and were similar for other ratios. Data of one representative experiment out of three.

Techniques Used: Expressing, Staining

Related Articles

Staining:

Article Title: PD-L1 Prevents the Development of Autoimmune Heart Disease in Graft-vs-Host Disease
Article Snippet: .. For intracellular cytokine staining, splenocytes were cultured for 1 hour in the presence of 20 ng/mL PMA (VWR Scientific) and 750 ng/mL ionomycin (VWR Scientific) and for an additional 2 hours in the presence of 10 ug/mL brefeldin A (Biolegend). .. Cells were treated with DNAse (Sigma), surface stained for CD4 and TS1 and treated with Cytofix/Cytoperm (BD).

other:

Article Title: Failure of Bay K 8644 to induce RhoA kinase-dependent calcium sensitization in rabbit blood vessels
Article Snippet: Ionomycin was from VWR (Bridgeport, NJ, USA).

Cell Culture:

Article Title: PD-L1 Prevents the Development of Autoimmune Heart Disease in Graft-vs-Host Disease
Article Snippet: .. For intracellular cytokine staining, splenocytes were cultured for 1 hour in the presence of 20 ng/mL PMA (VWR Scientific) and 750 ng/mL ionomycin (VWR Scientific) and for an additional 2 hours in the presence of 10 ug/mL brefeldin A (Biolegend). .. Cells were treated with DNAse (Sigma), surface stained for CD4 and TS1 and treated with Cytofix/Cytoperm (BD).

Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 92
    Avantor hepes
    Hepes, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/hepes/product/Avantor
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    hepes - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    92
    Avantor tris buffered saline tbs
    Tris Buffered Saline Tbs, supplied by Avantor, used in various techniques. Bioz Stars score: 92/100, based on 5 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tris buffered saline tbs/product/Avantor
    Average 92 stars, based on 5 article reviews
    Price from $9.99 to $1999.99
    tris buffered saline tbs - by Bioz Stars, 2020-09
    92/100 stars
      Buy from Supplier

    Image Search Results