ion torrent proton sequencer  (Thermo Fisher)


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    Name:
    Ion Proton System
    Description:
    The Ion Proton System is the first benchtop sequencing system capable of human scale genome exome or transcriptome sequencing in a few hours with DNA to variants called in a single day The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data By leveraging the exponential improvements in the semiconductor industry known as Moore s Law the Ion Proton Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications ranging from human scale genome to exome to transcriptome sequencing The system s use of the simplest natural sequencing chemistry eliminates the need for expensive optics and complex sequencing chemistries resulting in a highly affordable sequencing system to own and operate Real time direct electrical detection of sequencing combined with the enormous amount of computing power in both the Ion Proton Sequencer and Ion Proton Torrent Server enables generation of high quality sequencing results from DNA library to variants in a single day Features of the Ion Proton System • Fastest high throughput next generation sequencing workflow with the fastest sequencing run times of 2 4 hours on the Ion PI Chip • Highest number of complete sequencing runs per week with a simple and automated workflow when used with the Ion OneTouch 2 System • Simple semiconductor sequencing workflows with robust and simple hardware that can be relied upon no cameras no optics and no lasers • Scalable high throughput sequencing capabilities enabling up to 200 base single reads and flexible library options e g fragment gDNA targeted exome RNA • Small benchtop footprint and mounting capability with optional rack two Ion Proton systems per rack to deliver the only benchtop genome center • Low cost platform and attractively priced semiconductor chips and reagents for a range of applications • Proven Ion semiconductor sequencing requires no complex optics and employs natural nucleotides to deliver highly accurate variant detection uniformity of coverage and sensitivity to detect low frequency variants • Range of fast and simple library solutions and kits with low input requirements for a variety of applications such as genomic sequencing exome sequencing sequencing sets of genes and RNA sequencing • Complete end to end solution from base calls to variants with the Proton Torrent Server and Torrent Suite Software v3 0 • Simple and integrated tools for tertiary data analysis with Ion Reporter Software for DNA variation analysis across single paired or trio samples With the availability of the groundbreaking Ion Proton System rapid high throughput sequencing is finally accessible by all laboratories Affordable Sequencing For Nearly Every LabPrevious sequencing technologies have relied upon optical systems as bridges between the chemical and digital forms of sequence information These systems have added tremendous costs to sequencers relegating them to only the largest laboratories By eliminating the need for the optical system the Ion Proton System provides high throughput sequencing that is simpler faster more cost effective and more scalable than any other technology available With a benchtop footprint low cost sequencing reagents and a simple touchscreen user interface the Ion Proton System brings high throughput next generation sequencing to nearly every lab Ion Power PostLight Sequencing Produces Bits From BasesThe sequencing technology underlying the Ion Proton System exploits a well characterized biochemical process When a nucleotide is incorporated into a strand of DNA by a polymerase a hydrogen ion H is released as a byproduct This hydrogen ion carries a charge that the Proton System s ion sensor essentially the world s smallest solid state pH meter can detect As the sequencer floods the chip with one nucleotide after another any nucleotide added to a DNA template will be detected as a voltage change and the Ion Proton System will call the base If a nucleotide is not a match for a particular template no voltage change will be detected and no base will be called for that template Massively Parallel Sequencing On Your BenchA principal component of the Ion Proton System is the sequencing chip The Ion PI Chip incorporates an extremely dense array of 165 million micro machined wells married to our proprietary ion sensor Each well contains a different DNA template allowing massively parallel sequencing With the Ion PI Chip 165 million wells the Ion Proton System enables a range of high throughput genomic applications from human scale exome sequencing to whole transcriptome sequencing The Right Tool To Get Results Easily Reliably and QuicklyBecause it detects nucleotide incorporation without the use of light the Ion Proton System uses the simplest sequencing chemistry possible natural nucleotides There is no need for expensive and error prone modified bases enzymatic cascades chemiluminescence or fluorescence Direct detection also means the incorporation of each nucleotide is recorded in seconds As a result you can do an entire sequencing run typically in a few hours Services and Support Service and support plans comprehensive plans to maximize instrument performance and ensure availability of critical systems Qualification services document and ensure instrument is installed operating and performing to manufacturer s specifications Training courses for experimental design data analysis troubleshooting applications and instrument operation Bioinformatics tailored support for NGS informatics execution software use data analysis and downstream applications DataSafe Solution storage and back up strategy design hardware and support for all lab data
    Catalog Number:
    4476610
    Price:
    None
    Category:
    Instruments and Equipment
    Applications:
    DNA Sequencing|Epigenetic Sequencing|Fragment Analysis|Genotyping & Genomic Profiling|Ion Torrent™ Next-Generation Sequencing|Ion Torrent™ Systems & Accessories|Mitochondrial Sequencing|RNA Sequencing|SNP Genotyping by Fragment-Analysis|Sequencing|Small RNA & miRNA Sequencing|Methylation Analysis by Sequencing|Whole Genome Sequencing|Whole Transcriptome Sequencing|SNP Genotyping|Gene Expression Analysis & Genotyping|ChIP Sequencing|De Novo Sequencing|Gene Expression Profiling by Sequencing
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    Structured Review

    Thermo Fisher ion torrent proton sequencer
    The Ion Proton System is the first benchtop sequencing system capable of human scale genome exome or transcriptome sequencing in a few hours with DNA to variants called in a single day The system combines semiconductor sequencing technology with natural biochemistry to directly translate chemical information into digital data By leveraging the exponential improvements in the semiconductor industry known as Moore s Law the Ion Proton Sequencing System provides an unprecedented level of scalability and flexibility to support a broad range of high throughput sequencing applications ranging from human scale genome to exome to transcriptome sequencing The system s use of the simplest natural sequencing chemistry eliminates the need for expensive optics and complex sequencing chemistries resulting in a highly affordable sequencing system to own and operate Real time direct electrical detection of sequencing combined with the enormous amount of computing power in both the Ion Proton Sequencer and Ion Proton Torrent Server enables generation of high quality sequencing results from DNA library to variants in a single day Features of the Ion Proton System • Fastest high throughput next generation sequencing workflow with the fastest sequencing run times of 2 4 hours on the Ion PI Chip • Highest number of complete sequencing runs per week with a simple and automated workflow when used with the Ion OneTouch 2 System • Simple semiconductor sequencing workflows with robust and simple hardware that can be relied upon no cameras no optics and no lasers • Scalable high throughput sequencing capabilities enabling up to 200 base single reads and flexible library options e g fragment gDNA targeted exome RNA • Small benchtop footprint and mounting capability with optional rack two Ion Proton systems per rack to deliver the only benchtop genome center • Low cost platform and attractively priced semiconductor chips and reagents for a range of applications • Proven Ion semiconductor sequencing requires no complex optics and employs natural nucleotides to deliver highly accurate variant detection uniformity of coverage and sensitivity to detect low frequency variants • Range of fast and simple library solutions and kits with low input requirements for a variety of applications such as genomic sequencing exome sequencing sequencing sets of genes and RNA sequencing • Complete end to end solution from base calls to variants with the Proton Torrent Server and Torrent Suite Software v3 0 • Simple and integrated tools for tertiary data analysis with Ion Reporter Software for DNA variation analysis across single paired or trio samples With the availability of the groundbreaking Ion Proton System rapid high throughput sequencing is finally accessible by all laboratories Affordable Sequencing For Nearly Every LabPrevious sequencing technologies have relied upon optical systems as bridges between the chemical and digital forms of sequence information These systems have added tremendous costs to sequencers relegating them to only the largest laboratories By eliminating the need for the optical system the Ion Proton System provides high throughput sequencing that is simpler faster more cost effective and more scalable than any other technology available With a benchtop footprint low cost sequencing reagents and a simple touchscreen user interface the Ion Proton System brings high throughput next generation sequencing to nearly every lab Ion Power PostLight Sequencing Produces Bits From BasesThe sequencing technology underlying the Ion Proton System exploits a well characterized biochemical process When a nucleotide is incorporated into a strand of DNA by a polymerase a hydrogen ion H is released as a byproduct This hydrogen ion carries a charge that the Proton System s ion sensor essentially the world s smallest solid state pH meter can detect As the sequencer floods the chip with one nucleotide after another any nucleotide added to a DNA template will be detected as a voltage change and the Ion Proton System will call the base If a nucleotide is not a match for a particular template no voltage change will be detected and no base will be called for that template Massively Parallel Sequencing On Your BenchA principal component of the Ion Proton System is the sequencing chip The Ion PI Chip incorporates an extremely dense array of 165 million micro machined wells married to our proprietary ion sensor Each well contains a different DNA template allowing massively parallel sequencing With the Ion PI Chip 165 million wells the Ion Proton System enables a range of high throughput genomic applications from human scale exome sequencing to whole transcriptome sequencing The Right Tool To Get Results Easily Reliably and QuicklyBecause it detects nucleotide incorporation without the use of light the Ion Proton System uses the simplest sequencing chemistry possible natural nucleotides There is no need for expensive and error prone modified bases enzymatic cascades chemiluminescence or fluorescence Direct detection also means the incorporation of each nucleotide is recorded in seconds As a result you can do an entire sequencing run typically in a few hours Services and Support Service and support plans comprehensive plans to maximize instrument performance and ensure availability of critical systems Qualification services document and ensure instrument is installed operating and performing to manufacturer s specifications Training courses for experimental design data analysis troubleshooting applications and instrument operation Bioinformatics tailored support for NGS informatics execution software use data analysis and downstream applications DataSafe Solution storage and back up strategy design hardware and support for all lab data
    https://www.bioz.com/result/ion torrent proton sequencer/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ion torrent proton sequencer - by Bioz Stars, 2021-03
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    Related Articles

    RNA Sequencing Assay:

    Article Title: Comprehensive evaluation of AmpliSeq transcriptome, a novel targeted whole transcriptome RNA sequencing methodology for global gene expression analysis
    Article Snippet: .. The stimulated as well as unstimulated RNA samples from each cell line were prepared and sequenced using both the AmpliSeq and Proton RNA-seq on the Ion Torrent Proton. ..

    Next-Generation Sequencing:

    Article Title: Aldosterone-Producing Cell Clusters Frequently Harbor Somatic Mutations and Accumulate With Age in Normal Adrenals
    Article Snippet: Templates were prepared using an Ion PGM template OT2 200 kit or an Ion PI Hi-Q OT2 200 kit (Life Technologies) according to the manufacturer’s instruction. .. NGS was performed using an Ion PGM sequencing 200 kit v2 with Ion 318 chip on the Ion Torrent PGM sequencer, or Ion PI Hi-Q sequencing 200 kit with Ion PI chip v3 on the Ion Torrent Proton sequencer (Life Technologies). ..

    Article Title: Characterizing Benzo[a]pyrene-induced lacZ mutation spectrum in transgenic mice using next-generation sequencing
    Article Snippet: Amplification of the lacZ gene was carried out using the following thermocycle program: 95 °C for 3 min; 30 cycles of 95 °C for 45 s, 50 °C for 1 min, 72 °C for 4 min; final extension at 72 °C for 7 min. PCR products were purified using the QIAquick PCR purification kit (Qiagen) prior to library preparation. .. Ion Proton™ sequencing Next-generation sequencing of the lacZ mutant products was done in-house using an Ion Proton™ sequencer (Life Technologies, Carlsbad, CA). .. The Ion Xpress™ Plus Library Kits and AB Library Builder™ (Ion Xpress Plus Library Protocol v. 1.00) were used to prepare one library for each animal’s pool of mutants from purified PCR products (for a total of 24 libraries).

    Sequencing:

    Article Title: Aldosterone-Producing Cell Clusters Frequently Harbor Somatic Mutations and Accumulate With Age in Normal Adrenals
    Article Snippet: Templates were prepared using an Ion PGM template OT2 200 kit or an Ion PI Hi-Q OT2 200 kit (Life Technologies) according to the manufacturer’s instruction. .. NGS was performed using an Ion PGM sequencing 200 kit v2 with Ion 318 chip on the Ion Torrent PGM sequencer, or Ion PI Hi-Q sequencing 200 kit with Ion PI chip v3 on the Ion Torrent Proton sequencer (Life Technologies). ..

    Article Title: High-Throughput Screening of Tyrosine Kinase Inhibitor Cardiotoxicity with Human Induced Pluripotent Stem Cells
    Article Snippet: .. Transcriptome sequencing was conducted on an Ion Proton sequencing system (Life Technologies). .. For expression analysis of hiPSC-CMs following TKI treatment, a GeneChip® Human Gene 1.0 ST DNA Microarray was used (Affymetrix).

    Article Title: The IL-1B Gene Polymorphisms rs16944 and rs1143627 Contribute to an Increased Risk of Coronary Artery Lesions in Southern Chinese Children with Kawasaki Disease
    Article Snippet: .. Briefly, high-quality genomic DNA samples were genotyped by PCR using multiple gene-specific primer pairs to enrich the specific SNPs and indexing primers to enable massive parallel sequencing on the Ion Proton System (Life Technologies). ..

    Article Title: Truncating ARL6IP1 variant as the genetic cause of fatal complicated hereditary spastic paraplegia
    Article Snippet: Templated Ion Sphere particles were enriched using Ion OneTouch ES (Life Technologies, Carlsbad, CA, USA). .. The template-positive Ion PI Ion Sphere particles were processed for sequencing on the Ion Proton instrument (Life Technologies, Carlsbad, CA, USA); reads were mapped to UCSC hg19 ( http://genome.ucsc.edu/ ) and variants identified using the Ion Torrent pipeline (Life Technologies, Carlsbad, CA, USA). .. WES resulted in (on average) > 100x coverage totaling 46.2 Mb of genomic sequence from the index case.

    Article Title: Characterizing Benzo[a]pyrene-induced lacZ mutation spectrum in transgenic mice using next-generation sequencing
    Article Snippet: Amplification of the lacZ gene was carried out using the following thermocycle program: 95 °C for 3 min; 30 cycles of 95 °C for 45 s, 50 °C for 1 min, 72 °C for 4 min; final extension at 72 °C for 7 min. PCR products were purified using the QIAquick PCR purification kit (Qiagen) prior to library preparation. .. Ion Proton™ sequencing Next-generation sequencing of the lacZ mutant products was done in-house using an Ion Proton™ sequencer (Life Technologies, Carlsbad, CA). .. The Ion Xpress™ Plus Library Kits and AB Library Builder™ (Ion Xpress Plus Library Protocol v. 1.00) were used to prepare one library for each animal’s pool of mutants from purified PCR products (for a total of 24 libraries).

    Article Title: Limits and patterns of cytomegalovirus genomic diversity in humans
    Article Snippet: .. Pooled DNA was then prepared for sequencing on the Illumina HiSeq2000 as described previously ( ) or for sequencing on the ION proton sequencer (Life Technologies) using the manufacturer’s standard protocols and reagents. .. Samples were labeled with DNA barcodes and aggregated into libraries that generally included four to six samples.

    Chromatin Immunoprecipitation:

    Article Title: Aldosterone-Producing Cell Clusters Frequently Harbor Somatic Mutations and Accumulate With Age in Normal Adrenals
    Article Snippet: Templates were prepared using an Ion PGM template OT2 200 kit or an Ion PI Hi-Q OT2 200 kit (Life Technologies) according to the manufacturer’s instruction. .. NGS was performed using an Ion PGM sequencing 200 kit v2 with Ion 318 chip on the Ion Torrent PGM sequencer, or Ion PI Hi-Q sequencing 200 kit with Ion PI chip v3 on the Ion Torrent Proton sequencer (Life Technologies). ..

    Polymerase Chain Reaction:

    Article Title: The IL-1B Gene Polymorphisms rs16944 and rs1143627 Contribute to an Increased Risk of Coronary Artery Lesions in Southern Chinese Children with Kawasaki Disease
    Article Snippet: .. Briefly, high-quality genomic DNA samples were genotyped by PCR using multiple gene-specific primer pairs to enrich the specific SNPs and indexing primers to enable massive parallel sequencing on the Ion Proton System (Life Technologies). ..

    Mutagenesis:

    Article Title: Characterizing Benzo[a]pyrene-induced lacZ mutation spectrum in transgenic mice using next-generation sequencing
    Article Snippet: Amplification of the lacZ gene was carried out using the following thermocycle program: 95 °C for 3 min; 30 cycles of 95 °C for 45 s, 50 °C for 1 min, 72 °C for 4 min; final extension at 72 °C for 7 min. PCR products were purified using the QIAquick PCR purification kit (Qiagen) prior to library preparation. .. Ion Proton™ sequencing Next-generation sequencing of the lacZ mutant products was done in-house using an Ion Proton™ sequencer (Life Technologies, Carlsbad, CA). .. The Ion Xpress™ Plus Library Kits and AB Library Builder™ (Ion Xpress Plus Library Protocol v. 1.00) were used to prepare one library for each animal’s pool of mutants from purified PCR products (for a total of 24 libraries).

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  • 97
    Thermo Fisher non optical ion torrent sequencer
    Scalability of <t>Ion</t> <t>Torrent-compatible</t> MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton <t>sequencer</t> calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of <t>non-redundant</t> mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.
    Non Optical Ion Torrent Sequencer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/non optical ion torrent sequencer/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    non optical ion torrent sequencer - by Bioz Stars, 2021-03
    97/100 stars
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    97
    Thermo Fisher ngs sequencing kits
    The mean nucleotide percentage, N reads and average gap per sequencing method. The graph indicates a similarity in the nucleotide consistency between the sequencing methods. The SP method has an 8.9 and <t>SISPA</t> has a 10.3 times higher N reads compared to the <t>NGS</t> sequencer kit average. The Proton torrent had the least N reads, approximately 10 times lower than the other NGS sequencing kits. All the sequencing kits (Illumina, IonTorrrent and Proton Torrent) had the lowest N frequency.
    Ngs Sequencing Kits, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 97/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ngs sequencing kits/product/Thermo Fisher
    Average 97 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ngs sequencing kits - by Bioz Stars, 2021-03
    97/100 stars
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    Scalability of Ion Torrent-compatible MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton sequencer calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of non-redundant mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.

    Journal: Epigenetics

    Article Title: Semiconductor-based sequencing of genome-wide DNA methylation states

    doi: 10.1080/15592294.2014.1003747

    Figure Lengend Snippet: Scalability of Ion Torrent-compatible MeDIP-Seq protocol. (a) Global 5-methylcytosine (5mC) content measured as a percentage of the genome in DNA from WT and DKO cells determined by an ELISA assay. (b) Number of peaks identified by MACS v2.1.0 software 64 from MeDIP-Seq data in WT and DKO cells. (c) Sequence coverage of genome-wide CpGs for WT and DKO cells on the Ion Torrent Proton sequencer calculated using MEDIPS v1.14.0 software. 39 Pie charts illustrate the fraction of CpGs covered by the indicated reads according to their fold-coverage in relation to the total genomic CpG content from MeDIP-Seq libraries of the indicated sample sequenced on the Proton with the total number of non-redundant mapped reads in parentheses. (d) Sequence coverage of genome-wide CpGs for WT and DKO cells in MeDIP-Seq on the Ion Torrent PGM sequencer. Data displayed as in c . (e) Scaled chromosomal view of the 5mC MeDIP-Seq enrichment of methylation over the MX1 gene in WT and DKO cells sequenced on the 2 different Ion Torrent sequencers as indicated. MeDIP-Seq data are displayed as RPM (Reads Per Million mapped reads) below the RefSeq annotation of the gene (blue lines). Methylation enrichment is displayed in gray, with red vertical lines corresponding to areas containing CpGs. Chromosome position and gene orientation are displayed.

    Article Snippet: We therefore sought to determine how MeDIP-Seq data generated on a non-optical Ion Torrent sequencer (Ion Proton, Life Technologies) compares to that generated on an optical-based sequencing platform (GAIIx, Illumina).

    Techniques: Methylated DNA Immunoprecipitation, Enzyme-linked Immunosorbent Assay, Magnetic Cell Separation, Software, Sequencing, Genome Wide, Methylation

    The mean nucleotide percentage, N reads and average gap per sequencing method. The graph indicates a similarity in the nucleotide consistency between the sequencing methods. The SP method has an 8.9 and SISPA has a 10.3 times higher N reads compared to the NGS sequencer kit average. The Proton torrent had the least N reads, approximately 10 times lower than the other NGS sequencing kits. All the sequencing kits (Illumina, IonTorrrent and Proton Torrent) had the lowest N frequency.

    Journal: Gene: X

    Article Title: Evaluating methods for Avian avulavirus-1 whole genome sequencing

    doi: 10.1016/j.gene.2019.100004

    Figure Lengend Snippet: The mean nucleotide percentage, N reads and average gap per sequencing method. The graph indicates a similarity in the nucleotide consistency between the sequencing methods. The SP method has an 8.9 and SISPA has a 10.3 times higher N reads compared to the NGS sequencer kit average. The Proton torrent had the least N reads, approximately 10 times lower than the other NGS sequencing kits. All the sequencing kits (Illumina, IonTorrrent and Proton Torrent) had the lowest N frequency.

    Article Snippet: The viruses were sequenced using three different methods including the specific primers (34), SISPA (57) and 96 samples via NGS sequencing kits (8 IonTorrent, 84 Proton Torrent, 2 Illumina MiSeq and 2 Illumina NextSeq).

    Techniques: Sequencing, Next-Generation Sequencing

    Average coverage of each position in the AAvV-1 viral genome sequences, per method. The viruses were sequenced using three different methods including the specific primers (34), SISPA (57) and 96 samples via NGS sequencing kits (8 IonTorrent, 84 Proton Torrent, 4 Illumina). In all the methods a drop in the coverage near nucleotide 1700 is seen, possibly due to the G-quadruplexes. The Illumina and Proton Torrent have the highest coverage. The lowest coverage was obtained by the specific primers and SISPA methods (sequenced via the Ion Torrent).

    Journal: Gene: X

    Article Title: Evaluating methods for Avian avulavirus-1 whole genome sequencing

    doi: 10.1016/j.gene.2019.100004

    Figure Lengend Snippet: Average coverage of each position in the AAvV-1 viral genome sequences, per method. The viruses were sequenced using three different methods including the specific primers (34), SISPA (57) and 96 samples via NGS sequencing kits (8 IonTorrent, 84 Proton Torrent, 4 Illumina). In all the methods a drop in the coverage near nucleotide 1700 is seen, possibly due to the G-quadruplexes. The Illumina and Proton Torrent have the highest coverage. The lowest coverage was obtained by the specific primers and SISPA methods (sequenced via the Ion Torrent).

    Article Snippet: The viruses were sequenced using three different methods including the specific primers (34), SISPA (57) and 96 samples via NGS sequencing kits (8 IonTorrent, 84 Proton Torrent, 2 Illumina MiSeq and 2 Illumina NextSeq).

    Techniques: Next-Generation Sequencing, Sequencing