ion onetouch 2 system  (Thermo Fisher)


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    Name:
    Ion OneTouch 2 System
    Description:
    The Ion OneTouch 2 System performs template amplification and enrichment as part of the manual workflow for the Ion PGM, Ion Proton, and Ion S5 and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. With its small footprint, the system fits easily on any bench top. For a fully automated workflow, please see the Ion Chef Instrument. Efficiency and flexibility The Ion OneTouch 2 System integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM System, and up to 200 bases on the Ion Proton System. The need for gas is eliminated by the use of a peristaltic pump. System components The Ion OneTouch 2 System consists of two modules: the Ion OneTouch 2 Instrument and the Ion OneTouch ES (Enrichment System). The Ion OneTouch 2 Instrument may be purchased separately. Contributing technologies At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint. Designed to fit in any lab With the included Ion OneTouch ES module, the Ion OneTouch 2 System enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment. Services and support Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
    Catalog Number:
    4474779
    Price:
    None
    Applications:
    Ion Torrent Next-Generation Sequencing|Ion Torrent Systems & Accessories|Sequencing
    Size:
    1 system
    Category:
    Instruments and Equipment, Sample Prep Instruments for Sequencing
    Score:
    85
    Buy from Supplier


    Structured Review

    Thermo Fisher ion onetouch 2 system
    The Ion OneTouch 2 System performs template amplification and enrichment as part of the manual workflow for the Ion PGM, Ion Proton, and Ion S5 and Ion S5 XL systems. It provides scalable template preparation for all Ion semiconductor chips. With its small footprint, the system fits easily on any bench top. For a fully automated workflow, please see the Ion Chef Instrument. Efficiency and flexibility The Ion OneTouch 2 System integrates multiple manual template preparation steps into a single system. This enables parallel processing of multiple samples per day through modular design. It is flexible and supports scalable template preparation for all Ion semiconductor sequencing chips with up to 400 bases on the Ion 520/530 chips, up to 200 bases on the Ion 540 chip, up to 400 bases on the Ion PGM System, and up to 200 bases on the Ion Proton System. The need for gas is eliminated by the use of a peristaltic pump. System components The Ion OneTouch 2 System consists of two modules: the Ion OneTouch 2 Instrument and the Ion OneTouch ES (Enrichment System). The Ion OneTouch 2 Instrument may be purchased separately. Contributing technologies At the heart of the Ion OneTouch 2 Instrument are three breakthrough technologies that enable automated delivery of the template Ion Sphere particles. The first is the reaction filter that creates millions of microreactors in which clonal amplification occurs. The second is the fully integrated thermal cycler and disposable path amplification plate that enables robust thermal cycling of the microreactors. The third is the integrated centrifuge, which recovers the template Ion Sphere particles. Altogether this allows the instrument to deliver massively parallel clonal amplification and recovery, all in a small bench top footprint. Designed to fit in any lab With the included Ion OneTouch ES module, the Ion OneTouch 2 System enables parallel template processing with sample sequencing on the Ion PGM, Ion Proton, Ion S5, and Ion S5 XL systems. This reduces the time from sample to sequence to a single day and enables processing of multiple samples per day. The combination of Ion semiconductor sequencing and the Ion OneTouch 2 System maximizes the speed of each experiment and minimizes the overall time to completion for each project. The modular design also allows for QC of the Ion Sphere particles, to maximize experimental control and the success of each experiment. Services and support Service and support plans—comprehensive plans to maximize instrument performance and ensure availability of critical systems Qualification services—document and ensure instrument is installed, operating, and performing to manufacturer’s specifications Training—courses for experimental design, data analysis, troubleshooting, applications, and instrument operation Bioinformatics—tailored support for NGS informatics execution, software use, data analysis, and downstream applications DataSafe Solution—storage and back-up strategy, design, hardware, and support for all lab data
    https://www.bioz.com/result/ion onetouch 2 system/product/Thermo Fisher
    Average 99 stars, based on 99 article reviews
    Price from $9.99 to $1999.99
    ion onetouch 2 system - by Bioz Stars, 2019-10
    99/100 stars

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    Related Articles

    DNA Extraction:

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: Paragraph title: DNA extraction and targeted next generation sequencing (NGS) ... Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0.

    Article Title: Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate
    Article Snippet: Paragraph title: 2.2. DNA Extraction and Next-Generation Sequencing ... Then, multiplexed barcoded libraries were enriched by clonal amplification using emulsion PCR on an Ion OneTouch 2 system (Life Technologies, CA, USA) according to the manufacturer's protocol.

    Article Title: In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
    Article Snippet: Paragraph title: 4.10. DNA Extraction, 16S Amplicon Sequencing and Bioinformatics ... Then, emulsion PCR and enrichment were done using the Ion PGM Template OT2 400 Kit in Ion OneTouch 2 System (Thermo Scientific, Waltham, MA, USA).

    Amplification:

    Article Title: CDK4/6 inhibition triggers anti-tumor immunity
    Article Snippet: For each sample, 10 ng of total RNA was used for cDNA library preparation. .. Multiple libraries were multiplexed and clonally amplified using the Ion OneTouch 2 System (Thermo Fisher), and then sequenced on an Ion Torrent Proton machine (Thermo Fisher). .. Data was first analyzed by Torrent Suite and ampliSeqRNA analysis plugin (Thermo Fisher) to generate count data.

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: Briefly, 10 ng of total RNA samples from brain xenograft tumor tissue was used for cDNA library preparation. .. 8 libraries were multiplexed and clonally amplified by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton machine. .. Data was first analyzed by Torrent Suite and ampliSeqRNA analysis plugin (Life Technologies, Thermo Fisher) to generate count data.

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: The final exome libraries were quantitated by Ion Library Quantitation Kit (Life Technologies, Thermo Fisher). .. 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher). .. Alignment of sequencing reads was performed using Torrent Suite Software and Torrent Server.

    Article Title: Subclonal diversity arises early even in small colorectal tumours and contributes to differential growth fates
    Article Snippet: An equimolar mix of barcoded libraries was prepared and then diluted to 8 pM. .. The 8 pM library pool was used in preparation of template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Life Technologies). .. Template-positive ISPs were enriched using the Ion OneTouch ES all as described in the Ion PGM Template OT2 200 Kit User Guide, Publication #MAN0007220 Rev.

    Article Title: Whole genome amplification of cell-free DNA enables detection of circulating tumor DNA mutations from fingerstick capillary blood
    Article Snippet: 20 ng of input DNA was used for target amplification and library preparation using Ion AmpliSeq™ Library Kit 2.0 (Life Technologies) and the MammaSeqTM panel as described previously . .. Template preparation by emulsion PCR and enrichment was performed on the Ion OneTouch 2 system (Life Technologies).

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0. .. Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0.

    Article Title: Targeted mutation detection in breast cancer using MammaSeq™
    Article Snippet: Twenty nanograms of DNA (10 ng per amplicon pool) was used for library preparation using Ion AmpliSeq™ Library Kit 2.0 (ThermoFisher Scientific) and the custom designed MammaSeq™ primer panel (Additional file : Data file 1). .. Template preparation by emulsion PCR and enrichment was performed on the Ion OneTouch 2 system (ThermoFisher Scientific).

    Article Title: Application of a simplified method of chloroplast enrichment to small amounts of tissue for chloroplast genome sequencing
    Article Snippet: An Ion PGM Hi-Q OT2 Kit was used to prepare template-positive Ion Sphere Particles. .. Thermal cycling amplification of DNA fragments in microreactors was performed using the Ion OneTouch 2 system (Thermo Fisher Scientific). .. Positive particles were isolated and purified employing the Ion OneTouch ES system (Thermo Fisher Scientific).

    Article Title: Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate
    Article Snippet: Subsequently, we purified the libraries with AMPure XP beads (Beckman Coulter, Brea, CA, USA) and quantified the libraries by Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA). .. Then, multiplexed barcoded libraries were enriched by clonal amplification using emulsion PCR on an Ion OneTouch 2 system (Life Technologies, CA, USA) according to the manufacturer's protocol. .. The emulsion PCR was performed as follows: the enzyme was activated at 99°C for 2 min. DNA was denatured at 99°C for 15 seconds, and annealing and extension were performed at 60°C for 4 min for 17 cycles.

    Article Title: In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
    Article Snippet: Paragraph title: 4.10. DNA Extraction, 16S Amplicon Sequencing and Bioinformatics ... Then, emulsion PCR and enrichment were done using the Ion PGM Template OT2 400 Kit in Ion OneTouch 2 System (Thermo Scientific, Waltham, MA, USA).

    Article Title: PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
    Article Snippet: Undiluted amplified phage pools (5 μl) were used as templates for 24 cycles 50 μl PCR reactions using a distinct set of barcoded primers (0.5 μm each primer) for each reaction, and Phusion High Fidelity DNA polymerase (NEB) with a maximum polymerase concentration. .. Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: A Method for Amplicon Deep Sequencing of Drug Resistance Genes in Plasmodium falciparum Clinical Isolates from India
    Article Snippet: Paragraph title: Library preparation and amplicon sequencing. ... The library pool was templated on Ion Sphere particles using the Ion PGM Hi-Q OT2 kit on the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Article Title: Meta-analysis of genome-wide association studies identifies 8 novel loci involved in shape variation of human head hair
    Article Snippet: DNA (1–5 ng) was amplified in 10 μl of PCR reaction and one primer pool. .. Thirty-two of 100 pM DNA library samples were combined in equal ratios and subjected to template preparation with Ion PGM HiQ OT2 Kit and Ion OneTouch 2 System (Thermo Fisher Scientific).

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: After purification, libraries were quantified by a Qubit dsDNA HS Assay Kit and a Qubit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA), diluted to a concentration of 3 ng/mL, and pooled in equal volume. .. The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer. .. After enrichment, sequencing primers and the polymerase were added (PGM Sequencing Supplies 200 v2 Kit; Life Technologies).

    Construct:

    Article Title: CDK4/6 inhibition triggers anti-tumor immunity
    Article Snippet: AmpliSeq™ libraries were constructed and sequenced on the Ion Torrent Proton platform (Thermo Fisher) according to the manufacturer’s instructions as previously described , . .. Multiple libraries were multiplexed and clonally amplified using the Ion OneTouch 2 System (Thermo Fisher), and then sequenced on an Ion Torrent Proton machine (Thermo Fisher).

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: AmpliSeq human transcriptome libraries were constructed and sequenced in technical duplicate using the Ion Proton platform according to manufacturer instructions and as previously described . .. 8 libraries were multiplexed and clonally amplified by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton machine.

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: DNA libraries were constructed from 100 ng gDNA using the Ion AmpliSeq Exome kit (Life Technologies, Thermo Fisher) that provides targeted regions of greater than 97% the coding exons of the human genome. .. 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher).

    Article Title: Application of a simplified method of chloroplast enrichment to small amounts of tissue for chloroplast genome sequencing
    Article Snippet: Purified DNA (50 ng) was used to construct DNA fragment libraries using the Ion Xpress Plus Fragment Library Kit, following the manufacturer’s protocol (Thermo Fisher Scientific). .. Thermal cycling amplification of DNA fragments in microreactors was performed using the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Real-time Polymerase Chain Reaction:

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: Quantitative polymerase chain reaction (PCR) was performed by using an Mx3000P PCR system (Agilent, Santa Clara, CA, USA) with the following settings: 95°C for 10 minutes, 40 cycles at 95°C for 15 seconds, and 60°C for 1 minute. .. The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer.

    Quantitation Assay:

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: The final exome libraries were quantitated by Ion Library Quantitation Kit (Life Technologies, Thermo Fisher). .. 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher).

    Expressing:

    Article Title: CDK4/6 inhibition triggers anti-tumor immunity
    Article Snippet: For human gene analysis in cell lines, the Ion AmpliSeq Transcriptome Human Gene Expression Kit is designed for targeted amplification of over 20,000 human RefSeq genes simultaneously in a single primer pool. .. Multiple libraries were multiplexed and clonally amplified using the Ion OneTouch 2 System (Thermo Fisher), and then sequenced on an Ion Torrent Proton machine (Thermo Fisher).

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: 8 libraries were multiplexed and clonally amplified by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton machine. .. Count data were transformed using the R-bioconductor packages DESeq2 , then log2 transformed, then mean-normalized by gene to allow comparison.

    Transformation Assay:

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: 8 libraries were multiplexed and clonally amplified by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton machine. .. 8 libraries were multiplexed and clonally amplified by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton machine.

    Derivative Assay:

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: 8 libraries were multiplexed and clonally amplified by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton machine. .. Count data were transformed using the R-bioconductor packages DESeq2 , then log2 transformed, then mean-normalized by gene to allow comparison.

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: Total genomic DNA was extracted from tissue and sphere culture derived cells using the Blood & Cell Culture DNA Mini Kit (Qiagen, Hilden, Germany) following the handbook protocol (Tissue Protocol, 02/2003). .. Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0.

    Flow Cytometry:

    Article Title: LogMPIE, pan-India profiling of the human gut microbiome using 16S rRNA sequencing
    Article Snippet: The Ion OneTouch 2 system (Thermo Fisher Scientific, Carlsbad, CA, USA) enables automated delivery of Ion Sphere Particle (ISP) templates. .. The ISP templates were loaded either on an Ion 520 or Ion 530 chip kit, and a standardized protocol was followed.

    Ligation:

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: The libraries were bar coded during the ligation reaction using the Ion Xpress™ Barcode Adapter Kit (Life Technologies). .. Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0.

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: Briefly, pooled primers were used to amplify the genomic regions of the exons noted above, followed by ligation with adapters and barcodes. .. The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer.

    Cell Culture:

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: Total genomic DNA was extracted from tissue and sphere culture derived cells using the Blood & Cell Culture DNA Mini Kit (Qiagen, Hilden, Germany) following the handbook protocol (Tissue Protocol, 02/2003). .. Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0.

    Generated:

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: NGS libraries were generated with the Ion AmpliSeq™ Library Preparation Kit (Life Technologies) and our customized gene panel using ten nanograms of genomic DNA per primer pool for library preparation. .. Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0.

    Article Title: Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate
    Article Snippet: Ion Torrent adapter-ligated libraries were generated according to the Ion Ampliseq Library Kit 2.0 (Life Technologies, Carlsbad, CA, USA) protocol within about 5 hours. .. Then, multiplexed barcoded libraries were enriched by clonal amplification using emulsion PCR on an Ion OneTouch 2 system (Life Technologies, CA, USA) according to the manufacturer's protocol.

    Article Title: In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
    Article Snippet: 16S amplicons were generated with Phusion polymerase (New England BioLabs, Ipswich, MA, USA) with barcoded forward primers targeting 16S V3 region [ ] based on manufacturer’s protocols. .. Then, emulsion PCR and enrichment were done using the Ion PGM Template OT2 400 Kit in Ion OneTouch 2 System (Thermo Scientific, Waltham, MA, USA).

    Sequencing:

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: Paragraph title: Whole-exome sequencing ... 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher).

    Article Title: Subclonal diversity arises early even in small colorectal tumours and contributes to differential growth fates
    Article Snippet: Paragraph title: Targeted sequencing and variant calling ... The 8 pM library pool was used in preparation of template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Life Technologies).

    Article Title: Whole genome amplification of cell-free DNA enables detection of circulating tumor DNA mutations from fingerstick capillary blood
    Article Snippet: Paragraph title: Targeted sequencing ... Template preparation by emulsion PCR and enrichment was performed on the Ion OneTouch 2 system (Life Technologies).

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: The capture was performed with reagents from a custom design HaloPlex Target Enrichment kit (Agilent Technologies, Santa Clara, CA, USA), according to the HaloPlex Target Enrichment For Ion Torrent Sequencing Version D4. .. Template preparation, emulsion PCR, and Ion Sphere Particles (ISP) enrichment were carried out using the Ion PI™ Template OT2 200 Kit v2 on the Ion OneTouch™ 2 System (Thermo Fisher Scientific Inc.).

    Article Title: LogMPIE, pan-India profiling of the human gut microbiome using 16S rRNA sequencing
    Article Snippet: Paragraph title: Template Preparation and Sequencing ... The Ion OneTouch 2 system (Thermo Fisher Scientific, Carlsbad, CA, USA) enables automated delivery of Ion Sphere Particle (ISP) templates.

    Article Title: Shifts of subgingival bacterial population after nonsurgical and pharmacological therapy of localized aggressive periodontitis, followed for 1 year by Ion Torrent PGM platform
    Article Snippet: Paragraph title: Ion Torrent sequencing ... Template preparation was performed using the Ion PGM Template OT2 200 kit on Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA), after which the enrichment percentage was carried out on Qubit® 2.0 Fluorometer.

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0. .. Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0.

    Article Title: Targeted mutation detection in breast cancer using MammaSeq™
    Article Snippet: Paragraph title: Ion torrent sequencing ... Template preparation by emulsion PCR and enrichment was performed on the Ion OneTouch 2 system (ThermoFisher Scientific).

    Article Title: Application of a simplified method of chloroplast enrichment to small amounts of tissue for chloroplast genome sequencing
    Article Snippet: Paragraph title: High-throughput sequencing of fragmented DNA libraries ... Thermal cycling amplification of DNA fragments in microreactors was performed using the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Article Title: Next-generation sequencing identifies novel mutations in the FBN1 gene for two Chinese families with Marfan syndrome
    Article Snippet: The libraries of two patients with MFS were pooled together. .. Subsequent emulsion PCR and enrichment of the sequencing beads of the pooled libraries were performed using the Ion OneTouch 2 system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, as previously described ( ). .. Finally, 500 flows (125 cycles) of sequencing were performed on the Ion 318 Chip Kit v2 using an Ion PGM Sequencing 200 Kit v2 on the Ion PGM System (Thermo Fisher Scientific, Inc.).

    Article Title: Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate
    Article Snippet: Then, multiplexed barcoded libraries were enriched by clonal amplification using emulsion PCR on an Ion OneTouch 2 system (Life Technologies, CA, USA) according to the manufacturer's protocol. .. The emulsion PCR was performed as follows: the enzyme was activated at 99°C for 2 min. DNA was denatured at 99°C for 15 seconds, and annealing and extension were performed at 60°C for 4 min for 17 cycles.

    Article Title: In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
    Article Snippet: Paragraph title: 4.10. DNA Extraction, 16S Amplicon Sequencing and Bioinformatics ... Then, emulsion PCR and enrichment were done using the Ion PGM Template OT2 400 Kit in Ion OneTouch 2 System (Thermo Scientific, Waltham, MA, USA).

    Article Title: PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
    Article Snippet: Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA). .. Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: A Method for Amplicon Deep Sequencing of Drug Resistance Genes in Plasmodium falciparum Clinical Isolates from India
    Article Snippet: Paragraph title: Library preparation and amplicon sequencing. ... The library pool was templated on Ion Sphere particles using the Ion PGM Hi-Q OT2 kit on the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Article Title: Meta-analysis of genome-wide association studies identifies 8 novel loci involved in shape variation of human head hair
    Article Snippet: Thirty-two of 100 pM DNA library samples were combined in equal ratios and subjected to template preparation with Ion PGM HiQ OT2 Kit and Ion OneTouch 2 System (Thermo Fisher Scientific). .. Thirty-two of 100 pM DNA library samples were combined in equal ratios and subjected to template preparation with Ion PGM HiQ OT2 Kit and Ion OneTouch 2 System (Thermo Fisher Scientific).

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer. .. The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer.

    Multiplexing:

    Article Title: A Method for Amplicon Deep Sequencing of Drug Resistance Genes in Plasmodium falciparum Clinical Isolates from India
    Article Snippet: Ion Xpress barcode adapters 1 to 28 (Thermo Fisher Scientific) were used to facilitate multiplexing of 28 samples for each sequencing run. .. The library pool was templated on Ion Sphere particles using the Ion PGM Hi-Q OT2 kit on the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Mutagenesis:

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher). .. 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher).

    Article Title: Next-generation sequencing identifies novel mutations in the FBN1 gene for two Chinese families with Marfan syndrome
    Article Snippet: To recruit a maximum coverage of the mutation spectrum of MFS, a specific targeted resequencing panel was designed, including two genes predisposing to MFS, FBN1 and TGFBR2 ( ). .. Subsequent emulsion PCR and enrichment of the sequencing beads of the pooled libraries were performed using the Ion OneTouch 2 system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, as previously described ( ).

    Purification:

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: DNA was extracted from patient’s fibroblasts using NucleoSpin® Tissue XS (Machinery-Nagel, Germany) and purified using a QIAamp® DNA Micro Kit (Quiagen, Germany) following the manufacturers protocols. .. Template preparation, emulsion PCR, and Ion Sphere Particles (ISP) enrichment were carried out using the Ion PI™ Template OT2 200 Kit v2 on the Ion OneTouch™ 2 System (Thermo Fisher Scientific Inc.).

    Article Title: Application of a simplified method of chloroplast enrichment to small amounts of tissue for chloroplast genome sequencing
    Article Snippet: Purified DNA (50 ng) was used to construct DNA fragment libraries using the Ion Xpress Plus Fragment Library Kit, following the manufacturer’s protocol (Thermo Fisher Scientific). .. Thermal cycling amplification of DNA fragments in microreactors was performed using the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Article Title: Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate
    Article Snippet: Subsequently, we purified the libraries with AMPure XP beads (Beckman Coulter, Brea, CA, USA) and quantified the libraries by Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA). .. Then, multiplexed barcoded libraries were enriched by clonal amplification using emulsion PCR on an Ion OneTouch 2 system (Life Technologies, CA, USA) according to the manufacturer's protocol.

    Article Title: In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
    Article Snippet: After PCR, sharp DNA bands appeared at approximately 200 bp were cut and gel purified using QiaQuick Gel Extraction kit (Qiagen, Venlo, Netherlands) followed by AMPure treatment using the Agencourt AMPure XP system (Beckman Coulter, Brea, CA, USA). .. Then, emulsion PCR and enrichment were done using the Ion PGM Template OT2 400 Kit in Ion OneTouch 2 System (Thermo Scientific, Waltham, MA, USA).

    Article Title: A Method for Amplicon Deep Sequencing of Drug Resistance Genes in Plasmodium falciparum Clinical Isolates from India
    Article Snippet: A total of 500 ng of purified pooled PCR product from each sample was used to prepare fragment libraries using the NEBNext Fast DNA fragmentation and library prep set for Ion Torrent (New England BioLabs, MA, USA). .. The library pool was templated on Ion Sphere particles using the Ion PGM Hi-Q OT2 kit on the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: After purification, libraries were quantified by a Qubit dsDNA HS Assay Kit and a Qubit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA), diluted to a concentration of 3 ng/mL, and pooled in equal volume. .. The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer.

    Polymerase Chain Reaction:

    Article Title: Whole genome amplification of cell-free DNA enables detection of circulating tumor DNA mutations from fingerstick capillary blood
    Article Snippet: 20 ng of input DNA was used for target amplification and library preparation using Ion AmpliSeq™ Library Kit 2.0 (Life Technologies) and the MammaSeqTM panel as described previously . .. Template preparation by emulsion PCR and enrichment was performed on the Ion OneTouch 2 system (Life Technologies). .. Template positive Ion Sphere particles (ISP) were loaded onto P1 chip (cfDNA, 5000×) and 318 chips (buffy coat DNA, 500×), followed by sequencing on the Ion ProtonTM (Life Technologies) and Ion Torrent Personal Genome Machine (PGMTM ) (Life Technologies) respectively.

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: Libraries were quantified and qualified using the Qubit Fluorometer (Thermo Fisher Scientific Inc., USA) and the Agilent 2100 Bioanalyzer instrument (High Sensitivity DNA Kit) to enable equi-molar pooling of barcoded samples. .. Template preparation, emulsion PCR, and Ion Sphere Particles (ISP) enrichment were carried out using the Ion PI™ Template OT2 200 Kit v2 on the Ion OneTouch™ 2 System (Thermo Fisher Scientific Inc.). .. The quality of the ISPs was assessed using a Qubit 2.0 Fluorometer, and the ISPs were loaded and sequenced on a Ion PI™ Chip Kit v2 using Ion PI™ Sequencing 200 Kit v2 on the Ion Proton™ Sequencer (Thermo Fisher Scientific Inc.).

    Article Title: Targeted mutation detection in breast cancer using MammaSeq™
    Article Snippet: Twenty nanograms of DNA (10 ng per amplicon pool) was used for library preparation using Ion AmpliSeq™ Library Kit 2.0 (ThermoFisher Scientific) and the custom designed MammaSeq™ primer panel (Additional file : Data file 1). .. Template preparation by emulsion PCR and enrichment was performed on the Ion OneTouch 2 system (ThermoFisher Scientific). .. Template-positive Ion Sphere particles (ISP) were loaded onto Ion chips and sequenced.

    Article Title: Next-generation sequencing identifies novel mutations in the FBN1 gene for two Chinese families with Marfan syndrome
    Article Snippet: The libraries of two patients with MFS were pooled together. .. Subsequent emulsion PCR and enrichment of the sequencing beads of the pooled libraries were performed using the Ion OneTouch 2 system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, as previously described ( ). .. Finally, 500 flows (125 cycles) of sequencing were performed on the Ion 318 Chip Kit v2 using an Ion PGM Sequencing 200 Kit v2 on the Ion PGM System (Thermo Fisher Scientific, Inc.).

    Article Title: Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate
    Article Snippet: Subsequently, we purified the libraries with AMPure XP beads (Beckman Coulter, Brea, CA, USA) and quantified the libraries by Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA). .. Then, multiplexed barcoded libraries were enriched by clonal amplification using emulsion PCR on an Ion OneTouch 2 system (Life Technologies, CA, USA) according to the manufacturer's protocol. .. The emulsion PCR was performed as follows: the enzyme was activated at 99°C for 2 min. DNA was denatured at 99°C for 15 seconds, and annealing and extension were performed at 60°C for 4 min for 17 cycles.

    Article Title: In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
    Article Snippet: Sequencing library was prepared by pooling the 18 samples in equal molar ratio. .. Then, emulsion PCR and enrichment were done using the Ion PGM Template OT2 400 Kit in Ion OneTouch 2 System (Thermo Scientific, Waltham, MA, USA). .. The DNA sequencing was done using the Ion PGM Sequencing 400 Kit on Ion torrent PGM using Chip 318v2 in the Core Facilities of the Chinese University of Hong Kong.

    Article Title: PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
    Article Snippet: The PCR products were extracted using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) according to the manufacturer with the following exceptions: (a) Gel extracts were resolved at RT; (b) DNA was eluted with 30 μl low Tris-EDTA buffer (Thermo Fisher Scientific, Waltham, MA, USA). .. Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: PNPLA3 rs1010023 Predisposes Chronic Hepatitis B to Hepatic Steatosis but Improves Insulin Resistance and Glucose Metabolism
    Article Snippet: Thereafter, the custom Ion AmpliSeq panel (Life Technologies of Thermo Fisher Scientific, Waltham, MA, United States) of PNPLA3 was designed, with the overall coverage rate of 89.91%. .. Emulation PCR of the template was performed using the Ion OneTouch 2 System (Life Technologies of Thermo Fisher Scientific, Waltham, MA, United States) according to the manufacturer's instructions. .. PNPLA3 variants were genotyped by DNA sequencing using the Ion 318 Chip (Life Technologies of Thermo Fisher Scientific, Waltham, MA, United States) following the Ion PGM 200 Sequencing kit protocol.

    Article Title: A Method for Amplicon Deep Sequencing of Drug Resistance Genes in Plasmodium falciparum Clinical Isolates from India
    Article Snippet: A total of 500 ng of purified pooled PCR product from each sample was used to prepare fragment libraries using the NEBNext Fast DNA fragmentation and library prep set for Ion Torrent (New England BioLabs, MA, USA). .. The library pool was templated on Ion Sphere particles using the Ion PGM Hi-Q OT2 kit on the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Article Title: Detection of PIK3/AKT pathway in Moroccan population with triple negative breast cancer
    Article Snippet: Each library was barcoded using Ion Xpress™ Barcode Adapters kit (Ion Torrent; Thermo Fisher Scientific, Inc.). .. Template preparation by emulsion PCR was carried out using the Ion PGM™ Template Hi-Q OT2 200 kit, followed by the Ion OneTouch™ 2 System (Ion Torrent; Thermo Fisher Scientific, Inc.), according to manufacturers’ protocol. .. The polyclonal percentage and quality of the enriched, template-positive ISPs was determined using the Ion Sphere Quality Control Kit (Ion Torrent; Thermo Fisher Scientific, Inc.).

    Article Title: Meta-analysis of genome-wide association studies identifies 8 novel loci involved in shape variation of human head hair
    Article Snippet: DNA (1–5 ng) was amplified in 10 μl of PCR reaction and one primer pool. .. Thirty-two of 100 pM DNA library samples were combined in equal ratios and subjected to template preparation with Ion PGM HiQ OT2 Kit and Ion OneTouch 2 System (Thermo Fisher Scientific).

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: After purification, libraries were quantified by a Qubit dsDNA HS Assay Kit and a Qubit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA), diluted to a concentration of 3 ng/mL, and pooled in equal volume. .. The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer. .. After enrichment, sequencing primers and the polymerase were added (PGM Sequencing Supplies 200 v2 Kit; Life Technologies).

    Gel Extraction:

    Article Title: In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
    Article Snippet: After PCR, sharp DNA bands appeared at approximately 200 bp were cut and gel purified using QiaQuick Gel Extraction kit (Qiagen, Venlo, Netherlands) followed by AMPure treatment using the Agencourt AMPure XP system (Beckman Coulter, Brea, CA, USA). .. Then, emulsion PCR and enrichment were done using the Ion PGM Template OT2 400 Kit in Ion OneTouch 2 System (Thermo Scientific, Waltham, MA, USA).

    Article Title: PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
    Article Snippet: The PCR products were extracted using the QIAquick Gel Extraction Kit (Qiagen, Hilden, Germany) according to the manufacturer with the following exceptions: (a) Gel extracts were resolved at RT; (b) DNA was eluted with 30 μl low Tris-EDTA buffer (Thermo Fisher Scientific, Waltham, MA, USA). .. Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA).

    cDNA Library Assay:

    Article Title: CDK4/6 inhibition triggers anti-tumor immunity
    Article Snippet: For each sample, 10 ng of total RNA was used for cDNA library preparation. .. Multiple libraries were multiplexed and clonally amplified using the Ion OneTouch 2 System (Thermo Fisher), and then sequenced on an Ion Torrent Proton machine (Thermo Fisher).

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: Briefly, 10 ng of total RNA samples from brain xenograft tumor tissue was used for cDNA library preparation. .. 8 libraries were multiplexed and clonally amplified by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton machine.

    Chromatin Immunoprecipitation:

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: The final exome libraries were quantitated by Ion Library Quantitation Kit (Life Technologies, Thermo Fisher). .. 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher). .. Alignment of sequencing reads was performed using Torrent Suite Software and Torrent Server.

    Article Title: Subclonal diversity arises early even in small colorectal tumours and contributes to differential growth fates
    Article Snippet: The 8 pM library pool was used in preparation of template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Life Technologies). .. The 8 pM library pool was used in preparation of template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Life Technologies).

    Article Title: LogMPIE, pan-India profiling of the human gut microbiome using 16S rRNA sequencing
    Article Snippet: The Ion OneTouch 2 system (Thermo Fisher Scientific, Carlsbad, CA, USA) enables automated delivery of Ion Sphere Particle (ISP) templates. .. The Ion OneTouch 2 system (Thermo Fisher Scientific, Carlsbad, CA, USA) enables automated delivery of Ion Sphere Particle (ISP) templates.

    Article Title: Targeted mutation detection in breast cancer using MammaSeq™
    Article Snippet: Template preparation by emulsion PCR and enrichment was performed on the Ion OneTouch 2 system (ThermoFisher Scientific). .. Template preparation by emulsion PCR and enrichment was performed on the Ion OneTouch 2 system (ThermoFisher Scientific).

    Article Title: PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
    Article Snippet: Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA). .. Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Meta-analysis of genome-wide association studies identifies 8 novel loci involved in shape variation of human head hair
    Article Snippet: Thirty-two of 100 pM DNA library samples were combined in equal ratios and subjected to template preparation with Ion PGM HiQ OT2 Kit and Ion OneTouch 2 System (Thermo Fisher Scientific). .. Thirty-two of 100 pM DNA library samples were combined in equal ratios and subjected to template preparation with Ion PGM HiQ OT2 Kit and Ion OneTouch 2 System (Thermo Fisher Scientific).

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer. .. After enrichment, sequencing primers and the polymerase were added (PGM Sequencing Supplies 200 v2 Kit; Life Technologies).

    Software:

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher). .. Alignment of sequencing reads was performed using Torrent Suite Software and Torrent Server.

    Article Title: Subclonal diversity arises early even in small colorectal tumours and contributes to differential growth fates
    Article Snippet: The 8 pM library pool was used in preparation of template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Life Technologies). .. Enriched ISPs were loaded onto an Ion 318 Chip V.2 and sequenced with the Ion PGM Sequencing 200 Kit v2 on an Ion PGM System as described in the Ion PGM Sequencing 200 Kit V.2 User Guide, Publication #MAN0007273 Rev.

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: Template preparation, emulsion PCR, and Ion Sphere Particles (ISP) enrichment were carried out using the Ion PI™ Template OT2 200 Kit v2 on the Ion OneTouch™ 2 System (Thermo Fisher Scientific Inc.). .. The quality of the ISPs was assessed using a Qubit 2.0 Fluorometer, and the ISPs were loaded and sequenced on a Ion PI™ Chip Kit v2 using Ion PI™ Sequencing 200 Kit v2 on the Ion Proton™ Sequencer (Thermo Fisher Scientific Inc.).

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0. .. For Ion Torrent PGM™ sequencing, we used Ion 318™ chips and the Ion PGM™ HI-Q Sequencing Kit v2 (MAN0010863 Rev.B.0, Life Technologies).

    Article Title: In Vitro Infant Faecal Fermentation of Low Viscosity Barley β-Glucan and Its Acid Hydrolyzed Derivatives: Evaluation of Their Potential as Novel Prebiotics
    Article Snippet: Then, emulsion PCR and enrichment were done using the Ion PGM Template OT2 400 Kit in Ion OneTouch 2 System (Thermo Scientific, Waltham, MA, USA). .. The DNA sequencing was done using the Ion PGM Sequencing 400 Kit on Ion torrent PGM using Chip 318v2 in the Core Facilities of the Chinese University of Hong Kong.

    Article Title: PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
    Article Snippet: Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA). .. Sequencing was conducted on the Ion Torrent PGM sequencer using the Ion PGM Sequencing 200 Kit v2 and the Ion 314 Chip v2 (Thermo Fisher Scientific, Waltham, MA, USA) according to the manuals.

    Multiplex Assay:

    Article Title: Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate
    Article Snippet: Briefly, 20 ng gDNA for every sample was quantitated by Qubit 2.0 fluorometer (Invitrogen, Carlsbad, CA, USA) for multiplex PCR amplification with the 2X primer pools. .. Then, multiplexed barcoded libraries were enriched by clonal amplification using emulsion PCR on an Ion OneTouch 2 system (Life Technologies, CA, USA) according to the manufacturer's protocol.

    Selection:

    Article Title: A Method for Amplicon Deep Sequencing of Drug Resistance Genes in Plasmodium falciparum Clinical Isolates from India
    Article Snippet: Qualitative assessment of the libraries was carried out on the Agilent 2200 TapeStation system (Agilent Technologies, CA, USA) using high-sensitivity D1000 reagents (Agilent Technologies), to ensure proper size selection and absence of adaptor/primer dimers. .. The library pool was templated on Ion Sphere particles using the Ion PGM Hi-Q OT2 kit on the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Next-Generation Sequencing:

    Article Title: Ultrasonic Surgical Aspirate is a Reliable Source For Culturing Glioblastoma Stem Cells
    Article Snippet: Paragraph title: DNA extraction and targeted next generation sequencing (NGS) ... Emulsion-PCR was performed on the Ion OneTouch™ 2 System (Life Technologies) using the Ion PGM™ Hi-Q View OT2 Kit (MAN0014580 Rev.A.0.

    Article Title: Next-generation sequencing identifies novel mutations in the FBN1 gene for two Chinese families with Marfan syndrome
    Article Snippet: Paragraph title: MFS resequencing panel design and next-generation sequencing ... Subsequent emulsion PCR and enrichment of the sequencing beads of the pooled libraries were performed using the Ion OneTouch 2 system (Thermo Fisher Scientific, Inc.) according to the manufacturer's protocol, as previously described ( ).

    Article Title: Novel FGFR1 and KISS1R Mutations in Chinese Kallmann Syndrome Males with Cleft Lip/Palate
    Article Snippet: Paragraph title: 2.2. DNA Extraction and Next-Generation Sequencing ... Then, multiplexed barcoded libraries were enriched by clonal amplification using emulsion PCR on an Ion OneTouch 2 system (Life Technologies, CA, USA) according to the manufacturer's protocol.

    Article Title: Meta-analysis of genome-wide association studies identifies 8 novel loci involved in shape variation of human head hair
    Article Snippet: Samples were genotyped with next-generation sequencing using Ion AmpliSeqTM Technology and Ion PGMTM system (Thermo Fisher Scientific). .. Thirty-two of 100 pM DNA library samples were combined in equal ratios and subjected to template preparation with Ion PGM HiQ OT2 Kit and Ion OneTouch 2 System (Thermo Fisher Scientific).

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: The Ion Torrent system (Life Technologies) was used to analyze NGS libraries prepared by the NextDaySeq-lung Cancer Library Preparation Panel Kit (Beijing ACCB Biotech). .. The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer.

    Concentration Assay:

    Article Title: Shifts of subgingival bacterial population after nonsurgical and pharmacological therapy of localized aggressive periodontitis, followed for 1 year by Ion Torrent PGM platform
    Article Snippet: Template preparation was performed using the Ion PGM Template OT2 200 kit on Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA), after which the enrichment percentage was carried out on Qubit® 2.0 Fluorometer. .. Template preparation was performed using the Ion PGM Template OT2 200 kit on Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA), after which the enrichment percentage was carried out on Qubit® 2.0 Fluorometer.

    Article Title: PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
    Article Snippet: Undiluted amplified phage pools (5 μl) were used as templates for 24 cycles 50 μl PCR reactions using a distinct set of barcoded primers (0.5 μm each primer) for each reaction, and Phusion High Fidelity DNA polymerase (NEB) with a maximum polymerase concentration. .. Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA).

    Article Title: Genetic alterations and protein expression in combined small cell lung cancers and small cell lung cancers arising from lung adenocarcinomas after therapy with tyrosine kinase inhibitors
    Article Snippet: After purification, libraries were quantified by a Qubit dsDNA HS Assay Kit and a Qubit 2.0 fluorimeter (Life Technologies, Carlsbad, CA, USA), diluted to a concentration of 3 ng/mL, and pooled in equal volume. .. The pooled library was clonally amplified via emulsion PCR using ion sphere particles, and templatepositive particles were enriched by an Ion OneTouch 2 system (Life Technologies) as specified by the manufacturer.

    High Throughput Screening Assay:

    Article Title: Application of a simplified method of chloroplast enrichment to small amounts of tissue for chloroplast genome sequencing
    Article Snippet: Paragraph title: High-throughput sequencing of fragmented DNA libraries ... Thermal cycling amplification of DNA fragments in microreactors was performed using the Ion OneTouch 2 system (Thermo Fisher Scientific).

    Staining:

    Article Title: PP2A-B′ holoenzyme substrate recognition, regulation and role in cytokinesis
    Article Snippet: PCR reactions were supplemented with Gel Loading Dye Purple (6×; NEB) and separated on a 2.5% low-melt agarose (Bio-Rad, Hercules, CA, USA) gel stained with Roti-Safe GelStain (Carl-Roth, Karlsruhe, Germany). .. Template preparation was performed according to the manufacturer’s instruction using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Thermo Fisher Scientific, Waltham, MA, USA).

    Variant Assay:

    Article Title: Combination inhibition of PI3K and mTORC1 yields durable remissions in orthotopic patient-derived xenografts of HER2-positive breast cancer brain metastases
    Article Snippet: 2–3 libraries were multiplexed and clonally amplified to obtain template-positive ion sphere particles by using the Ion OneTouch 2 System (Life Technologies, Thermo Fisher), and were then sequenced on an Ion Torrent Proton using one PI chip kit V2 (Life Technologies, Thermo Fisher). .. Alignment of sequencing reads was performed using Torrent Suite Software and Torrent Server.

    Article Title: Subclonal diversity arises early even in small colorectal tumours and contributes to differential growth fates
    Article Snippet: Paragraph title: Targeted sequencing and variant calling ... The 8 pM library pool was used in preparation of template-positive Ion Sphere Particles (ISPs) containing clonally amplified DNA using the Ion PGM Template OT2 200 Kit on the Ion OneTouch 2 System (Life Technologies).

    Article Title: A Heterozygous ZMPSTE24 Mutation Associated with Severe Metabolic Syndrome, Ectopic Fat Accumulation, and Dilated Cardiomyopathy
    Article Snippet: Template preparation, emulsion PCR, and Ion Sphere Particles (ISP) enrichment were carried out using the Ion PI™ Template OT2 200 Kit v2 on the Ion OneTouch™ 2 System (Thermo Fisher Scientific Inc.). .. The coverage and sequencing depth analysis were computed using the BEDtools suite v2.17 [ ] and in-house scripts.

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    Thermo Fisher ion onetouch 2 system
    Ion Onetouch 2 System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 102 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ion onetouch 2 system/product/Thermo Fisher
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