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Thermo Fisher ion onetouch 2 system
Ion Onetouch 2 System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/ion onetouch 2 system/product/Thermo Fisher
Average 99 stars, based on 125 article reviews
Price from $9.99 to $1999.99
ion onetouch 2 system - by Bioz Stars, 2020-02
99/100 stars

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Related Articles

Multiplex Assay:

Article Title: Temporal development of the oral microbiome and prediction of early childhood caries
Article Snippet: .. Emulsion PCR of pooled amplicon libraries was performed using an Ion OneTouch™ 2 System with 400 bp protocols and multiplex sequencing was performed on an Ion Torrent Personal Genome Machine, utilising Hi-Q chemistry with 525 reagent flows per run (Thermo Fisher Scientific). .. Bioinformatics and statistical analyses Torrent Suite Software was used to demultiplex samples and trim sequencing adapters and barcodes.

Amplification:

Article Title: Temporal development of the oral microbiome and prediction of early childhood caries
Article Snippet: .. Emulsion PCR of pooled amplicon libraries was performed using an Ion OneTouch™ 2 System with 400 bp protocols and multiplex sequencing was performed on an Ion Torrent Personal Genome Machine, utilising Hi-Q chemistry with 525 reagent flows per run (Thermo Fisher Scientific). .. Bioinformatics and statistical analyses Torrent Suite Software was used to demultiplex samples and trim sequencing adapters and barcodes.

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: After assessing the correct size of the amplicon (260 bp) on a 2% acrylamide gel, the DNA quantification was performed by a Qubit® 2.0 Fluorimeter (Invitrogen, Carlsbad, CA, USA) and an equal amount of each sample was mixed to generate a pooled library at a final 100 pM concentration, according to manufacturer’s instructions. .. The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA).

Acrylamide Gel Assay:

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: After assessing the correct size of the amplicon (260 bp) on a 2% acrylamide gel, the DNA quantification was performed by a Qubit® 2.0 Fluorimeter (Invitrogen, Carlsbad, CA, USA) and an equal amount of each sample was mixed to generate a pooled library at a final 100 pM concentration, according to manufacturer’s instructions. .. The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA).

Mutagenesis:

Article Title: A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing, et al. A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing
Article Snippet: Paragraph title: 2.6. Detection of the mutation using NGS ... Emulsion PCR was performed using the Ion PGM Hi‐Q View OT2 Kit and the Ion OneTouch 2 system (Thermo Fisher Scientific Inc.), according to the manufacturer's instructions.

Chromatin Immunoprecipitation:

Article Title: A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing, et al. A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing
Article Snippet: Emulsion PCR was performed using the Ion PGM Hi‐Q View OT2 Kit and the Ion OneTouch 2 system (Thermo Fisher Scientific Inc.), according to the manufacturer's instructions. .. Enriched ISPs were loaded on a PGM 318 chip and sequenced using the Ion PGM Hi‐Q View Sequencing Reagents.

Next-Generation Sequencing:

Article Title: A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing, et al. A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing
Article Snippet: Paragraph title: 2.6. Detection of the mutation using NGS ... Emulsion PCR was performed using the Ion PGM Hi‐Q View OT2 Kit and the Ion OneTouch 2 system (Thermo Fisher Scientific Inc.), according to the manufacturer's instructions.

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: Paragraph title: 2.4. NGS Analyses ... The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA).

Nick Translation:

Article Title: Evaluating the cerebrospinal fluid ctDNA detection by next-generation sequencing in the diagnosis of meningeal Carcinomatosis
Article Snippet: AMPure beads (Beckman Coulter, Brea, CA) were used to purify adapter-ligated products, followed by nick-translation and PCR-amplification for a total of 5 cycles. .. Sample emulsion PCR and emulsion breaking were performed using the Ion OneTouch™2 system (Life Technologies) with the Ion PI Template OT2 200 Kit v3 (Life Technologies) as per the manufacturer’s instructions.

Size-exclusion Chromatography:

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: The PCR reactions were performed using the Kapa 2G HiFi Hotstart ready mix 2X (Kapa Biosystems, Wilmington, MA, USA) and BSA 400 ng/μL, under the following temperature cycling conditions: 95 °C for 5 min, 95 °C for 30 sec, 59 °C (V1–V3 PCR)/57 °C (V3 PCR) for 30 sec, and 72 °C for 45 sec with a maximum of 27 cycles for the V1–V3 PCR and a maximum of 13 cycles for the V3 PCR. .. The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA).

Purification:

Article Title: Temporal development of the oral microbiome and prediction of early childhood caries
Article Snippet: Purified barcoded amplicon libraries were quantified using a LabChip GX Touch 24 nucleic acids separation system using the DNA high sensitivity kits (Perkin Elmer). .. Emulsion PCR of pooled amplicon libraries was performed using an Ion OneTouch™ 2 System with 400 bp protocols and multiplex sequencing was performed on an Ion Torrent Personal Genome Machine, utilising Hi-Q chemistry with 525 reagent flows per run (Thermo Fisher Scientific).

Article Title: A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing, et al. A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing
Article Snippet: The DNA was bar‐coded using the Ion Xpress Barcode Adapters 1‐96 Kit and quantified by quantitative PCR (qPCR), using the Ion Library TaqMan® Quantitation Kit (Thermo Fisher Scientific Inc.) after purification of libraries. .. Emulsion PCR was performed using the Ion PGM Hi‐Q View OT2 Kit and the Ion OneTouch 2 system (Thermo Fisher Scientific Inc.), according to the manufacturer's instructions.

Real-time Polymerase Chain Reaction:

Article Title: A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing, et al. A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing
Article Snippet: The DNA was bar‐coded using the Ion Xpress Barcode Adapters 1‐96 Kit and quantified by quantitative PCR (qPCR), using the Ion Library TaqMan® Quantitation Kit (Thermo Fisher Scientific Inc.) after purification of libraries. .. Emulsion PCR was performed using the Ion PGM Hi‐Q View OT2 Kit and the Ion OneTouch 2 system (Thermo Fisher Scientific Inc.), according to the manufacturer's instructions.

Concentration Assay:

Article Title: Evaluating the cerebrospinal fluid ctDNA detection by next-generation sequencing in the diagnosis of meningeal Carcinomatosis
Article Snippet: And the Agilent 2100 Bioanalyzer and Agilent Bioanalyzer DNA High-Sensitivity LabChip (Agilent Technologies) were used to determine the concentration and size of the library. .. Sample emulsion PCR and emulsion breaking were performed using the Ion OneTouch™2 system (Life Technologies) with the Ion PI Template OT2 200 Kit v3 (Life Technologies) as per the manufacturer’s instructions.

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: After assessing the correct size of the amplicon (260 bp) on a 2% acrylamide gel, the DNA quantification was performed by a Qubit® 2.0 Fluorimeter (Invitrogen, Carlsbad, CA, USA) and an equal amount of each sample was mixed to generate a pooled library at a final 100 pM concentration, according to manufacturer’s instructions. .. The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA).

Quantitation Assay:

Article Title: A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing, et al. A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing
Article Snippet: The DNA was bar‐coded using the Ion Xpress Barcode Adapters 1‐96 Kit and quantified by quantitative PCR (qPCR), using the Ion Library TaqMan® Quantitation Kit (Thermo Fisher Scientific Inc.) after purification of libraries. .. Emulsion PCR was performed using the Ion PGM Hi‐Q View OT2 Kit and the Ion OneTouch 2 system (Thermo Fisher Scientific Inc.), according to the manufacturer's instructions.

Polymerase Chain Reaction:

Article Title: Temporal development of the oral microbiome and prediction of early childhood caries
Article Snippet: .. Emulsion PCR of pooled amplicon libraries was performed using an Ion OneTouch™ 2 System with 400 bp protocols and multiplex sequencing was performed on an Ion Torrent Personal Genome Machine, utilising Hi-Q chemistry with 525 reagent flows per run (Thermo Fisher Scientific). .. Bioinformatics and statistical analyses Torrent Suite Software was used to demultiplex samples and trim sequencing adapters and barcodes.

Article Title: Evaluating the cerebrospinal fluid ctDNA detection by next-generation sequencing in the diagnosis of meningeal Carcinomatosis
Article Snippet: .. Sample emulsion PCR and emulsion breaking were performed using the Ion OneTouch™2 system (Life Technologies) with the Ion PI Template OT2 200 Kit v3 (Life Technologies) as per the manufacturer’s instructions. .. Ion Sphere Particles (ISPs) were recovered, and template-positive ISPs were enriched with Dynabeads MyOne Streptavidin C1 beads (Life Technologies) on the Ion One Touch ES (enrichment system) (Life Technologies).

Article Title: A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing, et al. A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing
Article Snippet: .. Emulsion PCR was performed using the Ion PGM Hi‐Q View OT2 Kit and the Ion OneTouch 2 system (Thermo Fisher Scientific Inc.), according to the manufacturer's instructions. .. Template‐positive ion sphere particles (ISPs) were enriched using the Ion Torrent OneTouch ES (Thermo Fisher Scientific Inc.).

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: The PCR reactions were performed using the Kapa 2G HiFi Hotstart ready mix 2X (Kapa Biosystems, Wilmington, MA, USA) and BSA 400 ng/μL, under the following temperature cycling conditions: 95 °C for 5 min, 95 °C for 30 sec, 59 °C (V1–V3 PCR)/57 °C (V3 PCR) for 30 sec, and 72 °C for 45 sec with a maximum of 27 cycles for the V1–V3 PCR and a maximum of 13 cycles for the V3 PCR. .. The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA).

Polyacrylamide Gel Electrophoresis:

Article Title: Temporal development of the oral microbiome and prediction of early childhood caries
Article Snippet: PCRs were performed using a 50 μL reaction volume containing 6 ng of total DNA as template (consisting of ~3 ng bacterial DNA), 1X Q5 reaction buffer, 0.3 μM PAGE-purified custom barcoded primers (Thermo Fisher Scientific), 0.2 mM dNTPs (Thermo Fisher Scientific), and 1 unit of Q5 Hot Start High-Fidelity DNA polymerase. .. Emulsion PCR of pooled amplicon libraries was performed using an Ion OneTouch™ 2 System with 400 bp protocols and multiplex sequencing was performed on an Ion Torrent Personal Genome Machine, utilising Hi-Q chemistry with 525 reagent flows per run (Thermo Fisher Scientific).

High Throughput Screening Assay:

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA). .. Quantitative Insights Into Microbial Ecology (QIIME 1.9.1.) software, allowing analysis of high-throughput community sequencing data [ ] (available at: https://www.nature.com/articles/nmeth.f.303 ; accessed: 1st October 2019) was used to process the sequence data.

Software:

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA). .. Quantitative Insights Into Microbial Ecology (QIIME 1.9.1.) software, allowing analysis of high-throughput community sequencing data [ ] (available at: https://www.nature.com/articles/nmeth.f.303 ; accessed: 1st October 2019) was used to process the sequence data.

Sequencing:

Article Title: Temporal development of the oral microbiome and prediction of early childhood caries
Article Snippet: .. Emulsion PCR of pooled amplicon libraries was performed using an Ion OneTouch™ 2 System with 400 bp protocols and multiplex sequencing was performed on an Ion Torrent Personal Genome Machine, utilising Hi-Q chemistry with 525 reagent flows per run (Thermo Fisher Scientific). .. Bioinformatics and statistical analyses Torrent Suite Software was used to demultiplex samples and trim sequencing adapters and barcodes.

Article Title: Evaluating the cerebrospinal fluid ctDNA detection by next-generation sequencing in the diagnosis of meningeal Carcinomatosis
Article Snippet: Paragraph title: Ion proton library preparation and sequencing ... Sample emulsion PCR and emulsion breaking were performed using the Ion OneTouch™2 system (Life Technologies) with the Ion PI Template OT2 200 Kit v3 (Life Technologies) as per the manufacturer’s instructions.

Article Title: A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing, et al. A novel cell line generated using the CRISPR/Cas9 technology as universal quality control material for KRAS G12V mutation testing
Article Snippet: The sequencing library was prepared from 10 ng DNA using the Ion Torrent Ampliseq kit 2.0‐96 LV (Thermo Fisher Scientific Inc.), following the manufacturer's instructions. .. Emulsion PCR was performed using the Ion PGM Hi‐Q View OT2 Kit and the Ion OneTouch 2 system (Thermo Fisher Scientific Inc.), according to the manufacturer's instructions.

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA). .. Sequencing was performed with the Ion PGM™ System technology by using the Ion PGM Hi-Q View sequencing kit (Life Technologies, New York, USA).

Nested PCR:

Article Title: Introduction of NGS in Environmental Surveillance for Healthcare-Associated Infection Control
Article Snippet: A nested PCR was subsequently carried out with the primers B338F_P1-adaptor (B338F 5′-ACTCCTACGGGAGGCAGC-3′) and U534R_A_barcode, targeting the V3 region (200 bp) of the 16S rRNA gene, with a different barcode for each sample linked to the reverse primer [ ]. .. The Ion OneTouch™ 2 System (Life Technologies, Gran Island, New York, USA) was used to prepare the template with the Ion PGM Hi-Q View OT2 200 kit (Life Technologies, New York, USA).

Generated:

Article Title: Evaluating the cerebrospinal fluid ctDNA detection by next-generation sequencing in the diagnosis of meningeal Carcinomatosis
Article Snippet: For each sample type, an adapter-ligated library was generated with the Ion AmpliSeq Library Kit 2.0 (Life Technologies) according to the manufacturer’s protocol. .. Sample emulsion PCR and emulsion breaking were performed using the Ion OneTouch™2 system (Life Technologies) with the Ion PI Template OT2 200 Kit v3 (Life Technologies) as per the manufacturer’s instructions.

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  • 80
    Thermo Fisher ion onetouch 2 ot2 system
    Ion Onetouch 2 Ot2 System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ion onetouch 2 ot2 system/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ion onetouch 2 ot2 system - by Bioz Stars, 2020-02
    80/100 stars
      Buy from Supplier

    90
    Thermo Fisher ion onetouch 2 system
    Ion Onetouch 2 System, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 125 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ion onetouch 2 system/product/Thermo Fisher
    Average 90 stars, based on 125 article reviews
    Price from $9.99 to $1999.99
    ion onetouch 2 system - by Bioz Stars, 2020-02
    90/100 stars
      Buy from Supplier

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