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ti2 inverted widefield microscope  (Nikon)


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    Structured Review

    Nikon ti2 inverted widefield microscope
    Ti2 Inverted Widefield Microscope, supplied by Nikon, used in various techniques. Bioz Stars score: 99/100, based on 59420 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/invert+microscope/Objectives/pmc12989073-235-10-9
    Average 99 stars, based on 59420 article reviews
    ti2 inverted widefield microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Staining:

    Article Title: Caveolin-1, a Determinant of the Fate of MCF-7 Breast Cancer Cells
    Article Snippet: .. The staining solution was replaced by phosphate buffered saline (PBS) and images captured with Nikon invert microscope. ..

    Article Title: A biodegradable Zn–Se alloy with potent osteogenicity, antibacterial ability, and antitumor efficacy for bone-implant applications
    Article Snippet: After incubation for 14 d, the cells were fixed with 10 % paraformaldehyde solution for ALP (Beyotime, China) and ARS (Cyagen, China) staining following the manufacturers’ instructions. .. The stained images were recorded using an invert microscope (Nikon, Japan). .. In addition, the RNA of the cells was extracted using RNAiso Plus (TaKaRa, Japan) and then reversely transcribed into complementary DNA (cDNA) for subsequent qPCR assays.

    Saline:

    Article Title: Caveolin-1, a Determinant of the Fate of MCF-7 Breast Cancer Cells
    Article Snippet: .. The staining solution was replaced by phosphate buffered saline (PBS) and images captured with Nikon invert microscope. ..

    Microscopy:

    Article Title: Caveolin-1, a Determinant of the Fate of MCF-7 Breast Cancer Cells
    Article Snippet: .. The staining solution was replaced by phosphate buffered saline (PBS) and images captured with Nikon invert microscope. ..

    Article Title: Cultural optimization of pigeon muscle satellite cells and nutritional evaluation of the pigeon-derived cultivated meat
    Article Snippet: Cells were washed with PBS twice, then Bodipy working solution and 4',6-diamidino-2-phenylindole (DAPI) (C0060; Solarbio, Beijing, China) were added before incubation in the dark for 10 min at room temperature. .. Finally, the images were collected with an invert microscope (ECLIPSE Ti2; Nikon, Japan). ..

    Article Title: Effects of water fluoridation on early embryonic development of zebrafish.
    Article Snippet: .. Considering that, 30 embryos of each solution were randomly recorded using an invert microscope (ECLIPSE Ts2R-FL, Nikon, Tokyo, Japan) for analyzing the body lengths, the occupied areas of various organs in the body (e.g. pericardium and yolk sac), the diameters of eyes, and the head-body angles in different exposure scenario. ..

    Article Title: Development of an attenuated glutamine synthetase (GS) selection system for the stable expression of tissue plasminogen activator in CHO-K1 cells.
    Article Snippet: Chinese hamster ovary (CHO) cells represent the most common host system for the expression of high-quality recombinant proteins.. The development of stable CHO cell lines used in industrial recombinant protein production often relies on dihydrofolate reductase (DHFR) and glutamine synthetase (GS) amplification systems.. Conventional approaches to develop stable cell lines lead to heterogeneous cell populations.

    Article Title: Wafer-patterned, permeable, and stretchable liquid metal microelectrodes for implantable bioelectronics with chronic biocompatibility
    Article Snippet: .. For immunohistochemical analysis, slices were observed with the invert microscope (Nikon Eclipse Ti). ..

    Article Title: Cardiovascular developmental hazards of valproic acid in zebrafish.
    Article Snippet: Valproic acid (VPA) is predominantly prescribed for epilepsy, convulsions, and other psychiatric disorders.. As an epigenetic regulator, it is also used to treat various forms of cancer.. The clinical demand for the drug may pose an environmental hazard.

    Article Title: A biodegradable Zn–Se alloy with potent osteogenicity, antibacterial ability, and antitumor efficacy for bone-implant applications
    Article Snippet: After incubation for 14 d, the cells were fixed with 10 % paraformaldehyde solution for ALP (Beyotime, China) and ARS (Cyagen, China) staining following the manufacturers’ instructions. .. The stained images were recorded using an invert microscope (Nikon, Japan). .. In addition, the RNA of the cells was extracted using RNAiso Plus (TaKaRa, Japan) and then reversely transcribed into complementary DNA (cDNA) for subsequent qPCR assays.

    Article Title: Silibinin Attenuates Sulfur Mustard Analog-Induced Skin Injury by Targeting Multiple Pathways Connecting Oxidative Stress and Inflammation
    Article Snippet: .. Fluorescence of the DNA in cells and in comets seen in case of DNA damage was scored using a Nikon invert microscope (Nikon Eclipse TE300) at ×200 magnification, and images were captured using an attached CoolSNAP ES CCD camera. .. One hundred and fifty cells, 50 each on triplicate slides were captured and tail extent moment (TEM; product of tail length and percentage tail DNA) scored using Komet 5.5 software (ANDOR Technology, South Windsor, CT).

    Immunohistochemical staining:

    Article Title: Wafer-patterned, permeable, and stretchable liquid metal microelectrodes for implantable bioelectronics with chronic biocompatibility
    Article Snippet: .. For immunohistochemical analysis, slices were observed with the invert microscope (Nikon Eclipse Ti). ..

    Fluorescence:

    Article Title: Silibinin Attenuates Sulfur Mustard Analog-Induced Skin Injury by Targeting Multiple Pathways Connecting Oxidative Stress and Inflammation
    Article Snippet: .. Fluorescence of the DNA in cells and in comets seen in case of DNA damage was scored using a Nikon invert microscope (Nikon Eclipse TE300) at ×200 magnification, and images were captured using an attached CoolSNAP ES CCD camera. .. One hundred and fifty cells, 50 each on triplicate slides were captured and tail extent moment (TEM; product of tail length and percentage tail DNA) scored using Komet 5.5 software (ANDOR Technology, South Windsor, CT).



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    Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of <t>Brillouin</t> frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of Brillouin frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Embedded cell-only bioprinting to engineer structurally aligned meniscal fibrocartilage

    doi: 10.1016/j.mtbio.2026.103308

    Figure Lengend Snippet: Collagen alignment along the long axis of physical boundary is maintained despite YAP and ROCK inhibition. A) PLM imaging and color map analysis of collagen. B) Collagen intensity and coherency graphs. C) Immunohistochemistry for collagens I and II. Nuclei stained with hematoxylin. D) Heat map of Brillouin frequency shift. E) Brillouin frequency shift measurement. An ordinary one-way analysis of variance (ANOVA) with Tukey's multiple comparisons was used for statistical analysis (n = 3). Significance levels: ns = not significant, ∗∗∗∗p < 0.0001. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Measurements were acquired using an inverted Brillouin microscope (Discoverer, CellSense Technologies GmbH, Berlin, Germany) equipped with a 780 nm laser and a 20X air objective (Olympus, NA 0.50, UPlanFI).

    Techniques: Inhibition, Imaging, Immunohistochemistry, Staining