il2  (Thermo Fisher)


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    Name:
    Human Interleukin 2 IL 2 Recombinant Protein
    Description:
    Human Interleukin 2 IL 2 Recombinant Protein for Western Blot IP ELISA Ctrl
    Catalog Number:
    RIL2I
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    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher il2
    Plasmid treated mouse B cells, and not dendritic cells, are able to prime an antigen-specific CD8 T cell response in vivo a. Enriched B cells or DCs were obtained from HHDII-DR1 mice and treated with either 2μg/mL of the p103 peptide or 25μg/mL of pTVG-SSX2 DNA and intradermally transferred into naïve HHD-II/DR-I mice ( n = 6/treatment group). Two weeks later, splenocytes from the different treatment groups were assessed for antigen-specific CD8 T cells by intracellular cytokine staining for IFNγ (left) or <t>IL2</t> (right) expression following stimulation with control peptide, SSX2-derived p41 or p103 peptides, or PMA/Ionomycin (PMA, positive control). b. Pooled splenocytes were stimulated in vitro with peptide for one week prior to flow cytometric analysis. Shown are antigen-specific CD8 T cells expressing IFNγ (left), IL2 (center), or CD137/4-1BB (right) following re-stimulation. c. . Enriched B cells and DCs were treated with 25μg of psOVA and subcutaneously transferred into naïve C57BL6 mice ( n = 4-6/group). Another group received 100 μg psOVA alone intradermally. Two weeks later, splenocytes were analyzed for antigen-specific CD8 T cell responses to the SIINFEKL epitope by intracellular cytokine staining following in vitro stimulation as above. For all panels, data are corrected for background and * denotes a p -value
    Human Interleukin 2 IL 2 Recombinant Protein for Western Blot IP ELISA Ctrl
    https://www.bioz.com/result/il2/product/Thermo Fisher
    Average 95 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    il2 - by Bioz Stars, 2021-06
    95/100 stars

    Images

    1) Product Images from "B lymphocytes as direct antigen-presenting cells for anti-tumor DNA vaccines"

    Article Title: B lymphocytes as direct antigen-presenting cells for anti-tumor DNA vaccines

    Journal: Oncotarget

    doi: 10.18632/oncotarget.12178

    Plasmid treated mouse B cells, and not dendritic cells, are able to prime an antigen-specific CD8 T cell response in vivo a. Enriched B cells or DCs were obtained from HHDII-DR1 mice and treated with either 2μg/mL of the p103 peptide or 25μg/mL of pTVG-SSX2 DNA and intradermally transferred into naïve HHD-II/DR-I mice ( n = 6/treatment group). Two weeks later, splenocytes from the different treatment groups were assessed for antigen-specific CD8 T cells by intracellular cytokine staining for IFNγ (left) or IL2 (right) expression following stimulation with control peptide, SSX2-derived p41 or p103 peptides, or PMA/Ionomycin (PMA, positive control). b. Pooled splenocytes were stimulated in vitro with peptide for one week prior to flow cytometric analysis. Shown are antigen-specific CD8 T cells expressing IFNγ (left), IL2 (center), or CD137/4-1BB (right) following re-stimulation. c. . Enriched B cells and DCs were treated with 25μg of psOVA and subcutaneously transferred into naïve C57BL6 mice ( n = 4-6/group). Another group received 100 μg psOVA alone intradermally. Two weeks later, splenocytes were analyzed for antigen-specific CD8 T cell responses to the SIINFEKL epitope by intracellular cytokine staining following in vitro stimulation as above. For all panels, data are corrected for background and * denotes a p -value
    Figure Legend Snippet: Plasmid treated mouse B cells, and not dendritic cells, are able to prime an antigen-specific CD8 T cell response in vivo a. Enriched B cells or DCs were obtained from HHDII-DR1 mice and treated with either 2μg/mL of the p103 peptide or 25μg/mL of pTVG-SSX2 DNA and intradermally transferred into naïve HHD-II/DR-I mice ( n = 6/treatment group). Two weeks later, splenocytes from the different treatment groups were assessed for antigen-specific CD8 T cells by intracellular cytokine staining for IFNγ (left) or IL2 (right) expression following stimulation with control peptide, SSX2-derived p41 or p103 peptides, or PMA/Ionomycin (PMA, positive control). b. Pooled splenocytes were stimulated in vitro with peptide for one week prior to flow cytometric analysis. Shown are antigen-specific CD8 T cells expressing IFNγ (left), IL2 (center), or CD137/4-1BB (right) following re-stimulation. c. . Enriched B cells and DCs were treated with 25μg of psOVA and subcutaneously transferred into naïve C57BL6 mice ( n = 4-6/group). Another group received 100 μg psOVA alone intradermally. Two weeks later, splenocytes were analyzed for antigen-specific CD8 T cell responses to the SIINFEKL epitope by intracellular cytokine staining following in vitro stimulation as above. For all panels, data are corrected for background and * denotes a p -value

    Techniques Used: Plasmid Preparation, In Vivo, Mouse Assay, Staining, Expressing, Derivative Assay, Positive Control, In Vitro, Flow Cytometry

    2) Product Images from "JNK1 and ERK1/2 modulate lymphocyte homeostasis via BIM and DRP1 upon AICD induction"

    Article Title: JNK1 and ERK1/2 modulate lymphocyte homeostasis via BIM and DRP1 upon AICD induction

    Journal: Cell Death and Differentiation

    doi: 10.1038/s41418-020-0540-1

    Bim is required for AICD progression in normal and T-ALL cells. a Expression levels of the indicated proteins in naïve hPBT stimulated once, for 3 h (I stim), or twice (II stim) with anti-CD3 (plate-coated) and anti-CD28 antibodies. For the second stimulation, cells have been pre-activated with anti-CD3 (plate-coated) and anti-CD28 antibodies for 48 h and expanded for 6 days in IL2-containing medium. The quantification of the ratio between Bim-L/S protein isoforms and Bcl2 protein is reported for each condition in the graph on the right ( n = 3). b–f AICD has been induced in Jurkat cells silenced for Bim (checked by western blot in B, n = 3). Relative viability (AICD:unstimulated ratio) at 30 h upon AICD induction is indicated in C ( n = 6), western blot analysis of the Opa1 oligomers at 30 h in d ( n = 6; relative quantification on the right), TMRE staining at 26 h by flow cytometry in e ( n = 3; relative quantification on the right), and immunofluorescence analysis of mitochondria morphology (TOM20) and cyt-C localization at 30 h, in single confocal plane, in f ( n = 3; relative quantifications of percentage of cells with fragmented mitochondria, or released cyt-C, on the right). g Expression levels of the indicated genes from T-ALL and healthy bone marrows (HBM) obtained from Leukemia MILE Dataset and analyzed through BloodSpot (HBM: n = 73; T-ALL: n = 174). h Western blot analysis of Bim levels in healthy human Teff cells (stimulated once, and expanded 6 days with IL2), T-ALL patients and indicated T-ALL cell lines. Relative quantification of total Bim levels is reported in the graph on the right ( n = 3 controls; 4 patients; 5 cell lines ALL-SIL; TALL-1; RPMI-8402; P12-ICHIKAWA (P12-ICK); KOPT-K1). i Pie plots showing the mean percentage of viable (annV neg 7AAD neg ; in black), early apoptotic (annV pos 7AAD neg ; in dark gray) and late apoptotic cells (annV pos 7AAD pos ; in light gray) at the indicated time points after AICD induction in ALL-SIL, P12-ICHIKAWA (P12-ICK) and RPMI-8402 cells from three independent experiments ( n = 3). P values for the differences among the three cell lines at 32 h and 48 h after AICD induction are here indicated ( n = 3). At 32 h: early apoptotic SIL vs RPMI p
    Figure Legend Snippet: Bim is required for AICD progression in normal and T-ALL cells. a Expression levels of the indicated proteins in naïve hPBT stimulated once, for 3 h (I stim), or twice (II stim) with anti-CD3 (plate-coated) and anti-CD28 antibodies. For the second stimulation, cells have been pre-activated with anti-CD3 (plate-coated) and anti-CD28 antibodies for 48 h and expanded for 6 days in IL2-containing medium. The quantification of the ratio between Bim-L/S protein isoforms and Bcl2 protein is reported for each condition in the graph on the right ( n = 3). b–f AICD has been induced in Jurkat cells silenced for Bim (checked by western blot in B, n = 3). Relative viability (AICD:unstimulated ratio) at 30 h upon AICD induction is indicated in C ( n = 6), western blot analysis of the Opa1 oligomers at 30 h in d ( n = 6; relative quantification on the right), TMRE staining at 26 h by flow cytometry in e ( n = 3; relative quantification on the right), and immunofluorescence analysis of mitochondria morphology (TOM20) and cyt-C localization at 30 h, in single confocal plane, in f ( n = 3; relative quantifications of percentage of cells with fragmented mitochondria, or released cyt-C, on the right). g Expression levels of the indicated genes from T-ALL and healthy bone marrows (HBM) obtained from Leukemia MILE Dataset and analyzed through BloodSpot (HBM: n = 73; T-ALL: n = 174). h Western blot analysis of Bim levels in healthy human Teff cells (stimulated once, and expanded 6 days with IL2), T-ALL patients and indicated T-ALL cell lines. Relative quantification of total Bim levels is reported in the graph on the right ( n = 3 controls; 4 patients; 5 cell lines ALL-SIL; TALL-1; RPMI-8402; P12-ICHIKAWA (P12-ICK); KOPT-K1). i Pie plots showing the mean percentage of viable (annV neg 7AAD neg ; in black), early apoptotic (annV pos 7AAD neg ; in dark gray) and late apoptotic cells (annV pos 7AAD pos ; in light gray) at the indicated time points after AICD induction in ALL-SIL, P12-ICHIKAWA (P12-ICK) and RPMI-8402 cells from three independent experiments ( n = 3). P values for the differences among the three cell lines at 32 h and 48 h after AICD induction are here indicated ( n = 3). At 32 h: early apoptotic SIL vs RPMI p

    Techniques Used: Expressing, Western Blot, Staining, Flow Cytometry, Immunofluorescence

    Drugs targeting MAPK proteins reduces tumor growth in vivo and increases T cell survival. a, b 5*10 5 MC38 tumor cells have been inoculated s.c. into the right flank of WT mice. On the indicated days, mice have been injected i.p with SP600125 and FR18024, and tumor volume assessed ( a , n = 7 saline; n = 9 SP + FR-treated). After 14 days, TILs have been isolated from tumor mass and the percentage of CD8 + T cells among all CD45 + TILs quantified by flow cytometry ( b , n = 7 saline; n = 9 SP + FR-treated). c T cells have been isolated from the spleen of 14 days-old MC38-derived tumor-bearing WT mice and cultured in vitro for 10 days in presence of UV-irradiated MC38 cells, plus IL2 and IL15, to expand tumor-reactive T cells. After 10 days, CD8 + T cells have been magnetically purified and re-stimulated for the indicated time with plate-coated anti-CD3 antibodies to induce AICD in presence or not of SP600125 and/or FR180204. Graphs show the relative viabilities (AICD:unstimulated ratio) of murine T cells stimulated 6 h for AICD (percentage of annexinV neg 7AAD neg cells assessed by flow cytometry) ( n = 4). Data are shown as mean ± SEM. Significance is indicated as follows: * p
    Figure Legend Snippet: Drugs targeting MAPK proteins reduces tumor growth in vivo and increases T cell survival. a, b 5*10 5 MC38 tumor cells have been inoculated s.c. into the right flank of WT mice. On the indicated days, mice have been injected i.p with SP600125 and FR18024, and tumor volume assessed ( a , n = 7 saline; n = 9 SP + FR-treated). After 14 days, TILs have been isolated from tumor mass and the percentage of CD8 + T cells among all CD45 + TILs quantified by flow cytometry ( b , n = 7 saline; n = 9 SP + FR-treated). c T cells have been isolated from the spleen of 14 days-old MC38-derived tumor-bearing WT mice and cultured in vitro for 10 days in presence of UV-irradiated MC38 cells, plus IL2 and IL15, to expand tumor-reactive T cells. After 10 days, CD8 + T cells have been magnetically purified and re-stimulated for the indicated time with plate-coated anti-CD3 antibodies to induce AICD in presence or not of SP600125 and/or FR180204. Graphs show the relative viabilities (AICD:unstimulated ratio) of murine T cells stimulated 6 h for AICD (percentage of annexinV neg 7AAD neg cells assessed by flow cytometry) ( n = 4). Data are shown as mean ± SEM. Significance is indicated as follows: * p

    Techniques Used: In Vivo, Mouse Assay, Injection, Isolation, Flow Cytometry, Derivative Assay, Cell Culture, In Vitro, Irradiation, Purification

    Related Articles

    Recombinant:

    Article Title: IL-2/IL-2 Ab Therapy Induces Target Organ NK Cells that Inhibit CNS Inflammation
    Article Snippet: Anti-human IL-2 mAb was produced from DMS-1 hybridoma cells . .. Anti-human IL-2 Rβ mAb was purchased from R & D System, Inc. Carrier-free recombinant mouse and human IL-2 was purchased from eBioscience (San Diego, CA). .. For depletion of NK1.1+ cells or CD25+ cells in vivo, 100 μg anti-NK1.1 mAb or anti-CD25 mAb was injected i.p. into each mouse at day - 2 post immunization (p.i.).

    Incubation:

    Article Title: Improved expansion of T-cells in culture when isolated with an equipment-free, high-throughput, flow-through microfluidic module versus traditional density gradient centrifugation
    Article Snippet: After 24 hours, cells were washed by centrifugation at 250 × g for 10 minutes, re-suspended, and transferred to a new 6-well tissue culture plate. .. After 3 days, cells were washed and transferred to a new plate containing the same medium above, but with PHA replaced by 20 ng/mL human IL-2 (Gibco), and incubated for 7 days using standard proliferation techniques. .. Total numbers of viable cells were determined using an automated cell counter with Trypan Blue (Countess II, ThermoFisher).

    Enzyme-linked Immunosorbent Assay:

    Article Title: Immunization with Toxoplasma gondii GRA17 Deletion Mutant Induces Partial Protection and Survival in Challenged Mice
    Article Snippet: One million splenic cells collected from mice in different groups were seeded in triplicate wells in sterile 96-well flat-bottom tissue culture plates (Corning Incorporated, Corning, NY, USA) in a final volume of 200 µl RPMI supplemented with 10% heat-inactivated fetal calf serum, 2 mM glutamine, and 1% penicillin–streptomycin mixture. .. Culture supernatants were collected and the level of the secreted interleukin-2 (IL-2), IL-12, IL-10, and interferon gamma (IFN-γ) was determined using a commercial ELISA (eBioscience® Bender MedSystems GmbH, Austria). .. Challenge Models to Test the Efficacy of Immunization In these experiments, the efficacy of immunization with ΔGRA17 mutant strain was tested against acute, latent, and congenital infections in mice (Figure in Supplementary Material).

    Article Title: MERS-CoV Spike Protein Vaccine and Inactivated Influenza Vaccine Formulated with Single Strand RNA Adjuvant Induce T-Cell Activation through Intranasal Immunization in Mice
    Article Snippet: To re-stimulate the splenocytes, 500 ng/well of MERS S protein was added to the culture medium for two days, after which the medium was assessed with ELISA. .. The concentrations of interferon γ (IFN-γ), interleukin-2 (IL-2), IL-6, and tumor necrosis factor α (TNF-α) were detected with ELISA kits (Invitrogen; Thermo Fisher Scientific Inc., Waltham, MA, USA), according to the manufacturer’s instructions. ..

    Sequencing:

    Article Title: Autocrine/paracrine cytokine stimulation of leukemic cell proliferation in smoldering and chronic adult T-cell leukemia
    Article Snippet: .. The detection of human IL-2, IL-9, HTLV-1 Tax, and HPRT1 were performed using an ABI prism 7500 sequence detection system (Applied Biosystems) according to the manufacturer's instructions. ..

    Article Title: Interleukin-2 Functions in Anaplastic Large Cell Lymphoma Cells through Augmentation of Extracellular Signal-Regulated Kinases 1/2 Activation
    Article Snippet: The purified RNAs were denatured at 70°C for 10 min and reverse-transcribed with random hexamers at 42°C for 1 hour using a Superscript III kit (Invitrogen, Carlsbad, CA). .. The IL-2 gene was amplified using primers specific for the human IL-2 sequence (Applied Biosystems, Foster City, CA). .. The PCR reaction was performed using 37 cycles of denaturation at 94°C for 1 minute, annealing at 60°C for 1 minute, and elongation at 72°C for 1 minute as recommended by Applied Biosystems.

    Ex Vivo:

    Article Title: Signal transducer and activator of transcription 5 (STAT5) paralog dose governs T cell effector and regulatory functions
    Article Snippet: These were stimulated with plate-bound anti-CD3 (10 μg/ml; Clone 17A2) and soluble anti-CD28 (1 μg/ml; Clone 37.51) in the presence of soluble anti-mouse IL-2, IL-4 and IFN-ɣ (10 μg/ml each; Clones S4B6, BVD6-24G2 and XMG1.2; BioXcell, West Lebanon, NH) for 18 hr, then treated with human IL-2 (100 units/ml; NIH/NCI BRB Preclinical Repository) or mouse IL-6 (20 ng/ml; eBioscience) for 18 hr and stained with fluorochrome labelled anti-mouse CD25. .. For tyrosine-phosphorylated STAT5, splenocytes were treated directly ex vivo with human IL-2 (100 units/ml) or mouse IL-7 (20 ng/ml; eBioscience) for 1 hr, or stimulated with anti-CD3 and anti-CD28 in the presence of anti-mouse IL-2 for 18 hr, then pulsed with human IL-2 for 1 hr (100 units/ml). .. These were then fixed with 2% formaldehyde, permeabilized with 100% methanol and stained with Alexa Fluor 647-labelled anti-human/mouse pY694 STAT5 (Clone 47; BD Biosciences) in conjunction with fluorochrome labelled anti-mouse CD3ε, CD4, CD25, CD44, CD127 and/or FOXP3.

    Staining:

    Article Title: Evidence for HIV-1 cure after CCR5Δ32/Δ32 allogeneic haemopoietic stem-cell transplantation 30 months post analytical treatment interruption: a case report
    Article Snippet: In brief, cells were surface stained with antibodies (CD14 BV510, CD19 BV510, CD3 BV650, CD4 BV711, CD8 BV421; Biolegend, San Diego, CA, USA) in the presence of fixable Live/Dead stain (Invitrogen, Waltham, MA, USA). .. Cells were then fixed and permeabilised (CytoFix/CytoPerm; BD Biosciences) followed by intracellular cytokine staining for interferon γ with PE-Cyanine7, tumour necrosis factor with fluorescein isothiocyanate, and interleukin 2 with PercP eFluor710 (eBioscience, Waltham, MA, USA). .. Stimulation with 0·005% dimethyl sulphoxide in the presence of costimulatory antibodies, protein transport inhibitors, and CD107a was done as a negative control.

    Amplification:

    Article Title: Interleukin-2 Functions in Anaplastic Large Cell Lymphoma Cells through Augmentation of Extracellular Signal-Regulated Kinases 1/2 Activation
    Article Snippet: The purified RNAs were denatured at 70°C for 10 min and reverse-transcribed with random hexamers at 42°C for 1 hour using a Superscript III kit (Invitrogen, Carlsbad, CA). .. The IL-2 gene was amplified using primers specific for the human IL-2 sequence (Applied Biosystems, Foster City, CA). .. The PCR reaction was performed using 37 cycles of denaturation at 94°C for 1 minute, annealing at 60°C for 1 minute, and elongation at 72°C for 1 minute as recommended by Applied Biosystems.

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  • 98
    Thermo Fisher cytokine
    <t>Cytokine</t> production by DCs infected with ES
    Cytokine, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 98/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher interleukin 6
    Effect of CI SCFE on BLM-induced cytokines productions in lung tissues. The activities of tumor necrosis factor-alpha (TNF-α) ( A ); and interleukin <t>(IL-6)</t> ( B ) in lungs are presented as the mean ± SEM ( n = 8). # p
    Interleukin 6, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    94
    Thermo Fisher anti il 4
    Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, <t>anti-IL-4</t> and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P
    Anti Il 4, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    93
    Thermo Fisher il 10 fitc
    Rebamipide reciprocally regulates Th17/Treg cell imbalance. Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. a The tissues were stained with specific Abs against CD4 ( green ), CD25 ( white ), IL-17 ( red ), FOXP3 ( green ), p -STAT3–Y705 ( red ), and p -STAT3–S727 ( red ). Positive cells are shown in the bar graphs . b Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. Splenocytes were stained with Abs against CD4–PerCP, IL-17–PE, and <t>IL-10–FITC</t> to determine the presence of Th17 and IL-10-expressing Treg cells. Data are expressed as the mean ± SEM of 3 independent experiments (* P
    Il 10 Fitc, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Image Search Results


    Cytokine production by DCs infected with ES

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Enterobacter sakazakii targets DC-SIGN to induce immunosuppressive responses in dendritic cells by modulating MAP kinases

    doi: 10.4049/jimmunol.0902029

    Figure Lengend Snippet: Cytokine production by DCs infected with ES

    Article Snippet: Cytokine (TNF-α, IL-1β, IL-6, IL-12 p70, IL-10 and TGF-β) production in cell culture supernatants of DC-bacteria co-culture experiments collected after 24 and 48 h of incubation was carried out using Biosource ELISA kits (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.

    Techniques: Infection

    Effect of CI SCFE on BLM-induced cytokines productions in lung tissues. The activities of tumor necrosis factor-alpha (TNF-α) ( A ); and interleukin (IL-6) ( B ) in lungs are presented as the mean ± SEM ( n = 8). # p

    Journal: International Journal of Molecular Sciences

    Article Title: Supercritical-Carbon Dioxide Fluid Extract from Chrysanthemum indicum Enhances Anti-Tumor Effect and Reduces Toxicity of Bleomycin in Tumor-Bearing Mice

    doi: 10.3390/ijms18030465

    Figure Lengend Snippet: Effect of CI SCFE on BLM-induced cytokines productions in lung tissues. The activities of tumor necrosis factor-alpha (TNF-α) ( A ); and interleukin (IL-6) ( B ) in lungs are presented as the mean ± SEM ( n = 8). # p

    Article Snippet: Mouse tumor necrosis factor-α (TNF-α) and interleukin-6 (IL-6) the enzyme-linked immunosorbent assay (ELISA) reagents were purchased from eBioscience (San Diego, CA, USA); Myeloperoxidase (MPO) and malondiadehyde (MDA) Colorimetric Activity Assay Kits were obtained from Jiancheng Institution of Biotechnology (Nanjing, China).

    Techniques:

    Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, anti-IL-4 and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Coxsackievirus B3 Directly Induced Th17 Cell Differentiation by Inhibiting Nup98 Expression in Patients with Acute Viral Myocarditis

    doi: 10.3389/fcimb.2016.00171

    Figure Lengend Snippet: Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, anti-IL-4 and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P

    Article Snippet: After washing, cells were cultured with 1640 medium containing 5% FBS, 5 μg/mL of anti-CD3 (ebioscience), 2 μg/mL soluble anti-CD28 (eBioscience), 10 μg/mL anti-IL-4 (ebioscience), and 10 μg/mL anti-IFN-γ (ebioscience) for 5 days at room temperature.

    Techniques: Cell Differentiation, Infection, Transfection, Cell Culture, Flow Cytometry, Cytometry, FACS, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Rebamipide reciprocally regulates Th17/Treg cell imbalance. Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. a The tissues were stained with specific Abs against CD4 ( green ), CD25 ( white ), IL-17 ( red ), FOXP3 ( green ), p -STAT3–Y705 ( red ), and p -STAT3–S727 ( red ). Positive cells are shown in the bar graphs . b Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. Splenocytes were stained with Abs against CD4–PerCP, IL-17–PE, and IL-10–FITC to determine the presence of Th17 and IL-10-expressing Treg cells. Data are expressed as the mean ± SEM of 3 independent experiments (* P

    Journal: Journal of Translational Medicine

    Article Title: Rebamipide prevents peripheral arthritis and intestinal inflammation by reciprocally regulating Th17/Treg cell imbalance in mice with curdlan-induced spondyloarthritis

    doi: 10.1186/s12967-016-0942-5

    Figure Lengend Snippet: Rebamipide reciprocally regulates Th17/Treg cell imbalance. Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. a The tissues were stained with specific Abs against CD4 ( green ), CD25 ( white ), IL-17 ( red ), FOXP3 ( green ), p -STAT3–Y705 ( red ), and p -STAT3–S727 ( red ). Positive cells are shown in the bar graphs . b Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. Splenocytes were stained with Abs against CD4–PerCP, IL-17–PE, and IL-10–FITC to determine the presence of Th17 and IL-10-expressing Treg cells. Data are expressed as the mean ± SEM of 3 independent experiments (* P

    Article Snippet: Cells were permeabilized and fixed with CytoFix (BD Biosciences, San Jose, CA, USA), as instructed by the manufacturer, and were stained with Abs against IL-17–PE and IL-10–FITC (eBioscience).

    Techniques: Isolation, Mouse Assay, Injection, Staining, Expressing