interleukin 10  (Thermo Fisher)


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    Name:
    Human Interleukin 10 IL 10 Recombinant Protein
    Description:
    Human Interleukin 10 IL 10 Recombinant Protein for Western Blot IP ELISA Ctrl
    Catalog Number:
    RIL1025
    Price:
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    Category:
    Proteins Enzymes Peptides
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    Structured Review

    Thermo Fisher interleukin 10
    Parasite-specific <t>IL-10</t> and IL-4 production. BALB/c mice ( n = 8 per group) were vaccinated and infected as in Figure 4 . Spleen cell cultures from each mouse were independently established in the silent, initial, and late phases and stimulated for 72 h with soluble SLA or cultured without stimulus (medium). IL-10 ( a ) or IL-4 ( b ) levels were measured in culture supernatants by quantitative sandwich ELISA. Data are represented as the mean (+ SD) and statistics were analyzed by a Student T test. * ( p
    Human Interleukin 10 IL 10 Recombinant Protein for Western Blot IP ELISA Ctrl
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    Images

    1) Product Images from "Subcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Mice"

    Article Title: Subcutaneous Immunization of Leishmania HSP70-II Null Mutant Line Reduces the Severity of the Experimental Visceral Leishmaniasis in BALB/c Mice

    Journal: Vaccines

    doi: 10.3390/vaccines8010141

    Parasite-specific IL-10 and IL-4 production. BALB/c mice ( n = 8 per group) were vaccinated and infected as in Figure 4 . Spleen cell cultures from each mouse were independently established in the silent, initial, and late phases and stimulated for 72 h with soluble SLA or cultured without stimulus (medium). IL-10 ( a ) or IL-4 ( b ) levels were measured in culture supernatants by quantitative sandwich ELISA. Data are represented as the mean (+ SD) and statistics were analyzed by a Student T test. * ( p
    Figure Legend Snippet: Parasite-specific IL-10 and IL-4 production. BALB/c mice ( n = 8 per group) were vaccinated and infected as in Figure 4 . Spleen cell cultures from each mouse were independently established in the silent, initial, and late phases and stimulated for 72 h with soluble SLA or cultured without stimulus (medium). IL-10 ( a ) or IL-4 ( b ) levels were measured in culture supernatants by quantitative sandwich ELISA. Data are represented as the mean (+ SD) and statistics were analyzed by a Student T test. * ( p

    Techniques Used: Mouse Assay, Infection, Cell Culture, Sandwich ELISA

    2) Product Images from "Decreased Metabolic Flexibility in Skeletal Muscle of Rat Fed with a High-Fat Diet Is Recovered by Individual CLA Isomer Supplementation via Converging Protective Mechanisms"

    Article Title: Decreased Metabolic Flexibility in Skeletal Muscle of Rat Fed with a High-Fat Diet Is Recovered by Individual CLA Isomer Supplementation via Converging Protective Mechanisms

    Journal: Cells

    doi: 10.3390/cells9040823

    cis 9 , trans 11 (C9) or trans 10 , cis 12 (C10) isomer supplementation exhibits different efficacy in reducing proinflammatory markers and affected serum metabolic parameters of high fat diet (HFD)-fed rats. Proinflammatory cytokines, such as tumor necrosis factor (TNF-α) ( A ) and interleukin-1 (IL-1) ( B ), were measured in serum and in muscle (upper panels). Anti-inflammatory markers IL-10 were also determined in serum ( C ). Metabolic parameters, such as non-esterified fatty acids (NEFA) ( D ), leptin, adiponectin, and their ratio ( E – F ), glucose ( G ), insulin ( H ), and the HOMA index ( I ) are shown. Data were expressed as the means ± SEM n = 7 animals/group. Differing superscripted letters indicate statistically significant differences ( p
    Figure Legend Snippet: cis 9 , trans 11 (C9) or trans 10 , cis 12 (C10) isomer supplementation exhibits different efficacy in reducing proinflammatory markers and affected serum metabolic parameters of high fat diet (HFD)-fed rats. Proinflammatory cytokines, such as tumor necrosis factor (TNF-α) ( A ) and interleukin-1 (IL-1) ( B ), were measured in serum and in muscle (upper panels). Anti-inflammatory markers IL-10 were also determined in serum ( C ). Metabolic parameters, such as non-esterified fatty acids (NEFA) ( D ), leptin, adiponectin, and their ratio ( E – F ), glucose ( G ), insulin ( H ), and the HOMA index ( I ) are shown. Data were expressed as the means ± SEM n = 7 animals/group. Differing superscripted letters indicate statistically significant differences ( p

    Techniques Used:

    3) Product Images from "IOX1 activity as sepsis therapy and an antibiotic against multidrug-resistant bacteria"

    Article Title: IOX1 activity as sepsis therapy and an antibiotic against multidrug-resistant bacteria

    Journal: Scientific Reports

    doi: 10.1038/s41598-021-82377-z

    IOX1 antiseptic effect on an A. baumannii -inoculated septic mouse model. Six-week-old female BALB/c mice were intraperitoneally injected with IOX1 (20 mg/kg). After 30 min, the mice were i.p. injected with A. baumannii (KUMC ATCC 19606, 1.9 × 10 4 CFU/mouse). ( A ) The survival rates of IOX1- and A. baumannii -injected mice were monitored for 48 h. ( B ) The serum of mice was harvested 2 h after A. baumannii (9.5 × 10 3 CFU/mouse) injection in the presence or absence of IOX1. The levels of serum inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12p70 and IL-10) were measured by sandwich ELISA kits. ( C–G ) After overnight incubation, IOX1- and A. baumannii (9.5 × 10 3 CFU/mouse)-injected mice were sacrificed for experiments. ( C ) The mouse lungs were homogenized by stainless steel beads. The levels of lung inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12p70 and IL-10) were measured by sandwich ELISA kits. ( D ) PMN infiltrations in the lung were stained using the H E standard staining method. ( E ) The serum levels of AST, ALT, BUN and creatinine were measured by a laboratory medicine system. ( F ) The mouse lungs, livers, kidneys and spleens were homogenized by stainless steel beads. The lysates were diluted with PBS and incubated on LB agar plates overnight. ( G ) The serum levels of endotoxin were determined by the LAL method and measured at 405 nm.
    Figure Legend Snippet: IOX1 antiseptic effect on an A. baumannii -inoculated septic mouse model. Six-week-old female BALB/c mice were intraperitoneally injected with IOX1 (20 mg/kg). After 30 min, the mice were i.p. injected with A. baumannii (KUMC ATCC 19606, 1.9 × 10 4 CFU/mouse). ( A ) The survival rates of IOX1- and A. baumannii -injected mice were monitored for 48 h. ( B ) The serum of mice was harvested 2 h after A. baumannii (9.5 × 10 3 CFU/mouse) injection in the presence or absence of IOX1. The levels of serum inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12p70 and IL-10) were measured by sandwich ELISA kits. ( C–G ) After overnight incubation, IOX1- and A. baumannii (9.5 × 10 3 CFU/mouse)-injected mice were sacrificed for experiments. ( C ) The mouse lungs were homogenized by stainless steel beads. The levels of lung inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12p70 and IL-10) were measured by sandwich ELISA kits. ( D ) PMN infiltrations in the lung were stained using the H E standard staining method. ( E ) The serum levels of AST, ALT, BUN and creatinine were measured by a laboratory medicine system. ( F ) The mouse lungs, livers, kidneys and spleens were homogenized by stainless steel beads. The lysates were diluted with PBS and incubated on LB agar plates overnight. ( G ) The serum levels of endotoxin were determined by the LAL method and measured at 405 nm.

    Techniques Used: Mouse Assay, Injection, Sandwich ELISA, Incubation, Staining, AST Assay

    IOX1 antiseptic effect on a carbapenem resistant A. baumannii -inoculated septic mouse model. Six-week-old female BALB/c mice were intraperitoneally injected with IOX1 (20 mg/kg). After 30 min, the mice were i.p. injected with CRAB. ( A ) The survival rate of IOX1- and CRAB (1.4 × 10 4 CFU/mouse)-injected mice was monitored for 48 h. ( B ) The serum of IOX1- and CRAB (7 × 10 3 CFU/mouse)-injected mice was harvested 2 h after LPS injection. The serum levels of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12p70 and IL-10) were measured by sandwich ELISA kits. ( C–G ) After overnight incubation, IOX1- and CRAB (7 × 10 3 CFU/mouse)-injected mice were sacrificed for experiments. ( C ) The mouse lungs were homogenized by stainless steel beads. The lung levels of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12p70 and IL-10) were measured by sandwich ELISA kits. ( D ) The PMN infiltrations in the lung were stained by hematoxylin and eosin based on the standard H E staining method. ( E ) The serum levels of AST, ALT, BUN and creatinine were measured by a laboratory medicine system. ( F ) The mouse lungs, livers, kidneys and spleens were homogenized by stainless steel beads. The lysates were diluted with PBS and incubated on LB agar plates overnight. ( G ) The serum levels of endotoxin were determined by the LAL method and measured at 405 nm.
    Figure Legend Snippet: IOX1 antiseptic effect on a carbapenem resistant A. baumannii -inoculated septic mouse model. Six-week-old female BALB/c mice were intraperitoneally injected with IOX1 (20 mg/kg). After 30 min, the mice were i.p. injected with CRAB. ( A ) The survival rate of IOX1- and CRAB (1.4 × 10 4 CFU/mouse)-injected mice was monitored for 48 h. ( B ) The serum of IOX1- and CRAB (7 × 10 3 CFU/mouse)-injected mice was harvested 2 h after LPS injection. The serum levels of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12p70 and IL-10) were measured by sandwich ELISA kits. ( C–G ) After overnight incubation, IOX1- and CRAB (7 × 10 3 CFU/mouse)-injected mice were sacrificed for experiments. ( C ) The mouse lungs were homogenized by stainless steel beads. The lung levels of inflammatory cytokines (TNF-α, IL-1β, IL-6, IL-12p70 and IL-10) were measured by sandwich ELISA kits. ( D ) The PMN infiltrations in the lung were stained by hematoxylin and eosin based on the standard H E staining method. ( E ) The serum levels of AST, ALT, BUN and creatinine were measured by a laboratory medicine system. ( F ) The mouse lungs, livers, kidneys and spleens were homogenized by stainless steel beads. The lysates were diluted with PBS and incubated on LB agar plates overnight. ( G ) The serum levels of endotoxin were determined by the LAL method and measured at 405 nm.

    Techniques Used: Mouse Assay, Injection, Sandwich ELISA, Incubation, Staining, AST Assay

    IOX1 suppresses the inflammatory response in LPS-induced DC maturation. ( A ) Chemical structure of IOX1. ( B ) Mouse bone marrow-derived DCs were treated with the indicated concentrations of IOX1, DMSO or H 2 O 2 (negative control) overnight. The cytotoxicity of IOX1 in DCs was analyzed by a Luminescent Cell Viability Kit. ( C ) DCs treated with IOX1 (50 μM) before or after LPS stimulation (50 ng/ml) at the indicated times. Culture medium was collected, and the TNF-α, IL-1β, IL-6, IL-12p70 and IL-10 levels in the medium were determined by ELISA. ( D ) BMDCs were pretreated for 30 min with the indicated concentrations of IOX1 before stimulation with LPS (50 ng/ml) overnight. The surface molecule expression of BMDCs was analyzed by flow cytometry. The results of one representative experiment out of three experiments are shown. Data are presented as the means ± SEMs. * P
    Figure Legend Snippet: IOX1 suppresses the inflammatory response in LPS-induced DC maturation. ( A ) Chemical structure of IOX1. ( B ) Mouse bone marrow-derived DCs were treated with the indicated concentrations of IOX1, DMSO or H 2 O 2 (negative control) overnight. The cytotoxicity of IOX1 in DCs was analyzed by a Luminescent Cell Viability Kit. ( C ) DCs treated with IOX1 (50 μM) before or after LPS stimulation (50 ng/ml) at the indicated times. Culture medium was collected, and the TNF-α, IL-1β, IL-6, IL-12p70 and IL-10 levels in the medium were determined by ELISA. ( D ) BMDCs were pretreated for 30 min with the indicated concentrations of IOX1 before stimulation with LPS (50 ng/ml) overnight. The surface molecule expression of BMDCs was analyzed by flow cytometry. The results of one representative experiment out of three experiments are shown. Data are presented as the means ± SEMs. * P

    Techniques Used: Derivative Assay, Negative Control, Enzyme-linked Immunosorbent Assay, Expressing, Flow Cytometry

    4) Product Images from "Targeted Therapy for Acute Autoimmune Myocarditis with Nano-Sized Liposomal FK506 in Rats"

    Article Title: Targeted Therapy for Acute Autoimmune Myocarditis with Nano-Sized Liposomal FK506 in Rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0160944

    Effects of free and liposomal FK506 on inflammatory cytokines in the hearts of EAM rats. Cytokine expression on day 21 after immunization was evaluated using real-time quantitative PCR. Relative expression of IFN-γ (A), IL-17 (B), TNF-α (C), IL-1β (D), IL-10 (E), IL-4 (F) and TGF-β (G) were normalized to GAPDH (N = 4–9 in each group). Data are expressed as the mean ± SEM. * P
    Figure Legend Snippet: Effects of free and liposomal FK506 on inflammatory cytokines in the hearts of EAM rats. Cytokine expression on day 21 after immunization was evaluated using real-time quantitative PCR. Relative expression of IFN-γ (A), IL-17 (B), TNF-α (C), IL-1β (D), IL-10 (E), IL-4 (F) and TGF-β (G) were normalized to GAPDH (N = 4–9 in each group). Data are expressed as the mean ± SEM. * P

    Techniques Used: Expressing, Real-time Polymerase Chain Reaction

    5) Product Images from "Toll-like receptor 9 prevents cardiac rupture after myocardial infarction in mice independently of inflammation"

    Article Title: Toll-like receptor 9 prevents cardiac rupture after myocardial infarction in mice independently of inflammation

    Journal: American Journal of Physiology - Heart and Circulatory Physiology

    doi: 10.1152/ajpheart.00481.2016

    mRNA expression levels of interleukin-6 ( Il6 ; A ), tumor necrosis factor-α ( Tnfa ; B ), interleukin-1β ( Il1b ; C ), interleukin-10 ( Il10 ; D ), collagen type Iα2 ( Col1a2 ; E ), collagen type IIIα1 ( Col3a1 ; F ), matrix metalloproteinase ( Mmp ) 2 ( G ), Mmp9 ( H ), tissue inhibitor of metalloproteinase ( Timp ) 1 ( I ), Timp2 ( J ), Timp3 ( K ), and Timp4 , ( L ) corrected by Gapdh levels 1 and 3 days after myocardial infarction (MI) in WT and TLR9-KO (KO) hearts ( n = 4–7 mice for each group). Day 1: 1 day after MI; Day 3: 3 days after MI. M : the typical image ( left ) and the result of densitometoric analysis ( right , n = 3 mice for each group) of gelatin zymography using heart homogenates on Day 3. Data were analyzed by one-way ANOVA and are expressed as means ± SE * P
    Figure Legend Snippet: mRNA expression levels of interleukin-6 ( Il6 ; A ), tumor necrosis factor-α ( Tnfa ; B ), interleukin-1β ( Il1b ; C ), interleukin-10 ( Il10 ; D ), collagen type Iα2 ( Col1a2 ; E ), collagen type IIIα1 ( Col3a1 ; F ), matrix metalloproteinase ( Mmp ) 2 ( G ), Mmp9 ( H ), tissue inhibitor of metalloproteinase ( Timp ) 1 ( I ), Timp2 ( J ), Timp3 ( K ), and Timp4 , ( L ) corrected by Gapdh levels 1 and 3 days after myocardial infarction (MI) in WT and TLR9-KO (KO) hearts ( n = 4–7 mice for each group). Day 1: 1 day after MI; Day 3: 3 days after MI. M : the typical image ( left ) and the result of densitometoric analysis ( right , n = 3 mice for each group) of gelatin zymography using heart homogenates on Day 3. Data were analyzed by one-way ANOVA and are expressed as means ± SE * P

    Techniques Used: Expressing, Mouse Assay, Zymography

    6) Product Images from "Aldosterone Induces Renal Fibrosis and Inflammatory M1-Macrophage Subtype via Mineralocorticoid Receptor in Rats"

    Article Title: Aldosterone Induces Renal Fibrosis and Inflammatory M1-Macrophage Subtype via Mineralocorticoid Receptor in Rats

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0145946

    Quantitative analyses of protein levels measured by Western blot for Arg2 (A), ED2 (B) and SGK-1 (C) and mRNA levels measured by RT-PCR for iNOS (D), INFγ (E), Arg1 (F) and Il-10 (G) in control (CONTROL), spironolactone treated animals (SPIRO), aldosterone+salt-treated animals (ALDO) and aldosterone+salt plus spironolactone treated animals (ALDO+SPIRO). Data are expressed as mean ± SEM. *p
    Figure Legend Snippet: Quantitative analyses of protein levels measured by Western blot for Arg2 (A), ED2 (B) and SGK-1 (C) and mRNA levels measured by RT-PCR for iNOS (D), INFγ (E), Arg1 (F) and Il-10 (G) in control (CONTROL), spironolactone treated animals (SPIRO), aldosterone+salt-treated animals (ALDO) and aldosterone+salt plus spironolactone treated animals (ALDO+SPIRO). Data are expressed as mean ± SEM. *p

    Techniques Used: Western Blot, Reverse Transcription Polymerase Chain Reaction

    (A) Representative images showing CD68 staining in control+PBS liposome (Control+Lipo), aldosterone+PBS liposome (Aldo+Lipo), control+clodronate liposome (Control+Clodr), or aldosterone+clodronate liposome (Aldo+Clodr) treated animals. Quantitative analyses of CD68 protein (B) and mRNA (C) levels in treated rats. Quantitative analyses of Arg2 (D) and ED-2 (E) protein levels and IL-10 (F), Arg 1 (G) iNOS (H) and INFγ (I) mRNA expression in treated rats. Data are expressed as mean ± SEM. *p
    Figure Legend Snippet: (A) Representative images showing CD68 staining in control+PBS liposome (Control+Lipo), aldosterone+PBS liposome (Aldo+Lipo), control+clodronate liposome (Control+Clodr), or aldosterone+clodronate liposome (Aldo+Clodr) treated animals. Quantitative analyses of CD68 protein (B) and mRNA (C) levels in treated rats. Quantitative analyses of Arg2 (D) and ED-2 (E) protein levels and IL-10 (F), Arg 1 (G) iNOS (H) and INFγ (I) mRNA expression in treated rats. Data are expressed as mean ± SEM. *p

    Techniques Used: Staining, Expressing

    7) Product Images from "Physcion 8-O-β-glucopyranoside extracted from Polygonum cuspidatum exhibits anti-proliferative and anti-inflammatory effects on MH7A rheumatoid arthritis-derived fibroblast-like synoviocytes through the TGF-β/MAPK pathway"

    Article Title: Physcion 8-O-β-glucopyranoside extracted from Polygonum cuspidatum exhibits anti-proliferative and anti-inflammatory effects on MH7A rheumatoid arthritis-derived fibroblast-like synoviocytes through the TGF-β/MAPK pathway

    Journal: International Journal of Molecular Medicine

    doi: 10.3892/ijmm.2018.3649

    Inhibitory effects of POGD on levels of IL-1β, IL-6, IL-8, IL-10, IL-12 and IL-17A in TNF-α-stimulated MH7A cells. Cells were pretreated with TNF-α (10 ng/ml) for 12 h, following which the cells were exposed to POGD (8, 16 and 32 μ g/ml) for another 24 h; enzyme-linked immunosorbent assays were performed to determine the levels of cytokines in cell supernatants (n=4). ** P
    Figure Legend Snippet: Inhibitory effects of POGD on levels of IL-1β, IL-6, IL-8, IL-10, IL-12 and IL-17A in TNF-α-stimulated MH7A cells. Cells were pretreated with TNF-α (10 ng/ml) for 12 h, following which the cells were exposed to POGD (8, 16 and 32 μ g/ml) for another 24 h; enzyme-linked immunosorbent assays were performed to determine the levels of cytokines in cell supernatants (n=4). ** P

    Techniques Used:

    8) Product Images from "TSG-6 in extracellular vesicles from canine mesenchymal stem/stromal is a major factor in relieving DSS-induced colitis"

    Article Title: TSG-6 in extracellular vesicles from canine mesenchymal stem/stromal is a major factor in relieving DSS-induced colitis

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0220756

    cASC-EV TSG-6 increased Treg proliferation in vitro . Con A-stimulated canine lymphocytes were cocultured for 48 h with cASC-EVs transfected with TSG-6 siRNA (si-TSG6) or scrambled siRNA (siCTL), or naïve EVs. (A) CD4 and CD25 mRNA-expression levels were measured, confirming that TSG-6 was associated with increased Treg production. (B) IL-10, which is known to be secreted from Tregs, was also measured in the supernatant medium, and the results confirmed that IL-10 production in lymphocytes was associated with TSG-6 (n = 6 in each group). (C) The Treg population was determined by measuring FOXP3 and CD3 double-positive cells by flow cytometry (n = 6 in each group). The results are presented as the mean ± standard deviation (*P
    Figure Legend Snippet: cASC-EV TSG-6 increased Treg proliferation in vitro . Con A-stimulated canine lymphocytes were cocultured for 48 h with cASC-EVs transfected with TSG-6 siRNA (si-TSG6) or scrambled siRNA (siCTL), or naïve EVs. (A) CD4 and CD25 mRNA-expression levels were measured, confirming that TSG-6 was associated with increased Treg production. (B) IL-10, which is known to be secreted from Tregs, was also measured in the supernatant medium, and the results confirmed that IL-10 production in lymphocytes was associated with TSG-6 (n = 6 in each group). (C) The Treg population was determined by measuring FOXP3 and CD3 double-positive cells by flow cytometry (n = 6 in each group). The results are presented as the mean ± standard deviation (*P

    Techniques Used: In Vitro, Transfection, Expressing, Flow Cytometry, Standard Deviation

    cASC-EV TSG-6 induced macrophage polarization from M1 to M2 type in vitro. LPS-stimulated canine macrophage (DH82) were cocultured for 48 h with cASC-EVs transfected with TSG-6 siRNA (si-TSG6) or scrambled siRNA (siCTL), or naïve EVs. (A) TNF-α, IL-10, CD206 and Arg mRNA-expression levels were measured (C) The M1 and M2 population were determined by measuring CD206 and CD11c positive cells by flow cytometry (n = 6 in each group). The results are presented as the mean ± standard deviation (*P
    Figure Legend Snippet: cASC-EV TSG-6 induced macrophage polarization from M1 to M2 type in vitro. LPS-stimulated canine macrophage (DH82) were cocultured for 48 h with cASC-EVs transfected with TSG-6 siRNA (si-TSG6) or scrambled siRNA (siCTL), or naïve EVs. (A) TNF-α, IL-10, CD206 and Arg mRNA-expression levels were measured (C) The M1 and M2 population were determined by measuring CD206 and CD11c positive cells by flow cytometry (n = 6 in each group). The results are presented as the mean ± standard deviation (*P

    Techniques Used: In Vitro, Transfection, Expressing, Flow Cytometry, Standard Deviation

    9) Product Images from "Response of oxidative stress and inflammatory biomarkers to a 12-week aerobic exercise training in women with metabolic syndrome"

    Article Title: Response of oxidative stress and inflammatory biomarkers to a 12-week aerobic exercise training in women with metabolic syndrome

    Journal: Sports Medicine - Open

    doi: 10.1186/s40798-015-0011-2

    Effects on systemic cytokine levels. Aerobic training effects on interleukin-1 beta (IL-1β) (a), tumor necrosis factor alpha (TNF-α) (b) , interleukin-6 (IL-6) (c) , interferon-gamma (INF-γ) (d) , and interleukin-10 (IL-10) (e) levels. Data are expressed as means ± SD. * P
    Figure Legend Snippet: Effects on systemic cytokine levels. Aerobic training effects on interleukin-1 beta (IL-1β) (a), tumor necrosis factor alpha (TNF-α) (b) , interleukin-6 (IL-6) (c) , interferon-gamma (INF-γ) (d) , and interleukin-10 (IL-10) (e) levels. Data are expressed as means ± SD. * P

    Techniques Used:

    IL-1β, TNF-α , IFN-γ, IL-10, IRS-2, and MMP-9 mRNA expression in PBMC. Box plots of aerobic training effects on interleukin-1 beta (IL-1β) (a) , tumor necrosis factor alpha (TNF-α) (b) , interferon-gamma (IFN)-γ (c) , interleukin-10 (IL-10) (d) , insulin receptor substrate 2 (IRS-2) (e) , and matrix metalloproteinase-9 (MMP-9) mRNA expression in PBMC of eight women with metabolic syndrome. Values are normalized to β-actin mRNA expression. Data are expressed as median, interquartile range, and whiskers extending to the 5th and 95th percentiles. * P
    Figure Legend Snippet: IL-1β, TNF-α , IFN-γ, IL-10, IRS-2, and MMP-9 mRNA expression in PBMC. Box plots of aerobic training effects on interleukin-1 beta (IL-1β) (a) , tumor necrosis factor alpha (TNF-α) (b) , interferon-gamma (IFN)-γ (c) , interleukin-10 (IL-10) (d) , insulin receptor substrate 2 (IRS-2) (e) , and matrix metalloproteinase-9 (MMP-9) mRNA expression in PBMC of eight women with metabolic syndrome. Values are normalized to β-actin mRNA expression. Data are expressed as median, interquartile range, and whiskers extending to the 5th and 95th percentiles. * P

    Techniques Used: Expressing

    10) Product Images from "FTY720 inhibits tubulointerstitial inflammation in albumin overload-induced nephropathy of rats via the Sphk1 pathway"

    Article Title: FTY720 inhibits tubulointerstitial inflammation in albumin overload-induced nephropathy of rats via the Sphk1 pathway

    Journal: Acta Pharmacologica Sinica

    doi: 10.1038/aps.2014.100

    Effect of FTY720 on inflammatory cytokines, including monocyte chemoattractant protein-1 (MCP-1), macrophage-related TNF-α and IL-6, arginase-1 and IL-10, in the three groups. (A) Western blot analysis of MCP-1 and M1 and M2 macrophage-related
    Figure Legend Snippet: Effect of FTY720 on inflammatory cytokines, including monocyte chemoattractant protein-1 (MCP-1), macrophage-related TNF-α and IL-6, arginase-1 and IL-10, in the three groups. (A) Western blot analysis of MCP-1 and M1 and M2 macrophage-related

    Techniques Used: Western Blot

    11) Product Images from "Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo)"

    Article Title: Phenolic Compounds Isolated from Calea uniflora Less. Promote Anti-Inflammatory and Antioxidant Effects in Mice Neutrophils (Ex Vivo) and in Mice Pleurisy Model (In Vivo)

    Journal: Mediators of Inflammation

    doi: 10.1155/2019/1468502

    Effect of noreugenin or α -hydroxy-butein upon IL-1 β (a), IL-17A (b), and IL-10 (c) levels in carrageenan-induced inflammation in the mouse model of pleurisy. Control = animals treated with saline solution (NaCl 0.9%) only. Cg = animals treated with carrageenan (1%) only. Dex = animals pretreated with dexamethasone (0.5 mg/kg, i.p.). NRG = noreugenin (5 mg/kg, i.p.). AH-BU = α -hydroxy-butein (2.5 mg/kg, i.p.). Bars represent the mean ± S.E.M. of 5 animals. The values in brackets represent the percentages of inhibition. ∗ p
    Figure Legend Snippet: Effect of noreugenin or α -hydroxy-butein upon IL-1 β (a), IL-17A (b), and IL-10 (c) levels in carrageenan-induced inflammation in the mouse model of pleurisy. Control = animals treated with saline solution (NaCl 0.9%) only. Cg = animals treated with carrageenan (1%) only. Dex = animals pretreated with dexamethasone (0.5 mg/kg, i.p.). NRG = noreugenin (5 mg/kg, i.p.). AH-BU = α -hydroxy-butein (2.5 mg/kg, i.p.). Bars represent the mean ± S.E.M. of 5 animals. The values in brackets represent the percentages of inhibition. ∗ p

    Techniques Used: Inhibition

    12) Product Images from "Cardiomyogenic Differentiation of Human Dental Follicle-derived Stem Cells by Suberoylanilide Hydroxamic Acid and Their In Vivo Homing Property"

    Article Title: Cardiomyogenic Differentiation of Human Dental Follicle-derived Stem Cells by Suberoylanilide Hydroxamic Acid and Their In Vivo Homing Property

    Journal: International Journal of Medical Sciences

    doi: 10.7150/ijms.16573

    Comparative analysis of the serum levels of IL-2 and IL-10 in the systemic iCM injected mice and controls. The serum levels of IL-2 and IL-10 in the experimental mice (14 days after IP injection of iCM) were not different compared to those of the two control groups.
    Figure Legend Snippet: Comparative analysis of the serum levels of IL-2 and IL-10 in the systemic iCM injected mice and controls. The serum levels of IL-2 and IL-10 in the experimental mice (14 days after IP injection of iCM) were not different compared to those of the two control groups.

    Techniques Used: Injection, Mouse Assay

    13) Product Images from "Enhanced immunity in a mouse model of malignant glioma is mediated by a therapeutic ketogenic diet"

    Article Title: Enhanced immunity in a mouse model of malignant glioma is mediated by a therapeutic ketogenic diet

    Journal: BMC Cancer

    doi: 10.1186/s12885-016-2337-7

    The ketogenic diet significantly enhances tumor-reactive CD8+ T cell and NK cell activity. Tumor-infiltrating lymphocytes (TILs) isolated from gliomas from mice fed KD versus SD were cultured alone (white bar) or in the presence of GL261-Luc2 tumor cells (black bar) to access activity. Analysis of IFNγ, TNF and IL-2 production in tumor-infiltrating CD8+ T cells was performed ( a ). Cytotoxic capability of CD8+ T cells isolated from tumors was assessed following exposure to GL261-Luc2 cells ( b ). IL-10-production in CD4 + FoxP3+ T regulatory cells was also assessed in response to stimulation with GL261-Luc2 cells ( c ). IFNγ and TNF production in the infiltrating NKp46 + CD3- natural killer cells isolated from tumors were assessed ( d ). N = 5; student’s two-tailed t -test between the antigen-challenged SD and KD groups only; * p
    Figure Legend Snippet: The ketogenic diet significantly enhances tumor-reactive CD8+ T cell and NK cell activity. Tumor-infiltrating lymphocytes (TILs) isolated from gliomas from mice fed KD versus SD were cultured alone (white bar) or in the presence of GL261-Luc2 tumor cells (black bar) to access activity. Analysis of IFNγ, TNF and IL-2 production in tumor-infiltrating CD8+ T cells was performed ( a ). Cytotoxic capability of CD8+ T cells isolated from tumors was assessed following exposure to GL261-Luc2 cells ( b ). IL-10-production in CD4 + FoxP3+ T regulatory cells was also assessed in response to stimulation with GL261-Luc2 cells ( c ). IFNγ and TNF production in the infiltrating NKp46 + CD3- natural killer cells isolated from tumors were assessed ( d ). N = 5; student’s two-tailed t -test between the antigen-challenged SD and KD groups only; * p

    Techniques Used: Activity Assay, Isolation, Mouse Assay, Cell Culture, Two Tailed Test

    14) Product Images from "PLK:Δgra9 Live Attenuated Strain Induces Protective Immunity Against Acute and Chronic Toxoplasmosis"

    Article Title: PLK:Δgra9 Live Attenuated Strain Induces Protective Immunity Against Acute and Chronic Toxoplasmosis

    Journal: Frontiers in Microbiology

    doi: 10.3389/fmicb.2021.619335

    Δ gra9 vaccination activates the ability of the splenocytes to rapidly and specifically recognize the Toxoplasma antigen to induce high-level cytokines, compared with unvaccinated. Immunological memory of mice in Δ gra9 vaccination was evaluated at 70 dpv via stimulated splenocytes by total Toxoplasma soluble antigen resulting in the production of cytokines IFN-γ (A) or IL-10 (B) . RPMI 1640 with 20% FBS only or 5 μg/ml concanavalin A were used as negative or positive controls, respectively. The data are presented as the mean ± SEM of at least three repeats each sample (**** P
    Figure Legend Snippet: Δ gra9 vaccination activates the ability of the splenocytes to rapidly and specifically recognize the Toxoplasma antigen to induce high-level cytokines, compared with unvaccinated. Immunological memory of mice in Δ gra9 vaccination was evaluated at 70 dpv via stimulated splenocytes by total Toxoplasma soluble antigen resulting in the production of cytokines IFN-γ (A) or IL-10 (B) . RPMI 1640 with 20% FBS only or 5 μg/ml concanavalin A were used as negative or positive controls, respectively. The data are presented as the mean ± SEM of at least three repeats each sample (**** P

    Techniques Used: Mouse Assay

    Δ gra9 vaccination provides safe and effective immune protection. The peritoneal fluids and sera in challenged mice were collected at 7 days tachyzoite post-challenges or 14 days cyst post-challenges to determine cytokine productions and T. gondii specific IgG, compared with unvaccinated but secondly challenged mouse samples. (A) IFN-γ, IL-12p70, or IL-10 levels in serum or peritoneal fluid samples. –, unvaccinated; +, vaccinated (* P
    Figure Legend Snippet: Δ gra9 vaccination provides safe and effective immune protection. The peritoneal fluids and sera in challenged mice were collected at 7 days tachyzoite post-challenges or 14 days cyst post-challenges to determine cytokine productions and T. gondii specific IgG, compared with unvaccinated but secondly challenged mouse samples. (A) IFN-γ, IL-12p70, or IL-10 levels in serum or peritoneal fluid samples. –, unvaccinated; +, vaccinated (* P

    Techniques Used: Mouse Assay

    15) Product Images from "IL-10 Overexpression Alters Survival in the Setting of Gram Negative Pneumonia Following Lung Contusion"

    Article Title: IL-10 Overexpression Alters Survival in the Setting of Gram Negative Pneumonia Following Lung Contusion

    Journal: Shock (Augusta, Ga.)

    doi: 10.1097/SHK.0000000000000123

    Evaluation of lung parenchyma macrophages isolated from IL-10 OE and TG- mice 30 hours after lung contusion and 24 hours after pneumonia for macrophage phenotype and evidence of intracellular bacterial killing. (A) Macrophages were isolated by plastic
    Figure Legend Snippet: Evaluation of lung parenchyma macrophages isolated from IL-10 OE and TG- mice 30 hours after lung contusion and 24 hours after pneumonia for macrophage phenotype and evidence of intracellular bacterial killing. (A) Macrophages were isolated by plastic

    Techniques Used: Isolation, Mouse Assay

    Effect of lung contusion on expression of Arginase 1 in BAL and lung parenchyma macrophages isolated from IL-10 overexpression and control mice. All mice received 5 days of doxycycline chow prior to experimental use. (A) Macrophages were isolated from
    Figure Legend Snippet: Effect of lung contusion on expression of Arginase 1 in BAL and lung parenchyma macrophages isolated from IL-10 overexpression and control mice. All mice received 5 days of doxycycline chow prior to experimental use. (A) Macrophages were isolated from

    Techniques Used: Expressing, Isolation, Over Expression, Mouse Assay

    Effects of lung-specific IL-10 overexpression on alveolar cytokine/chemokine levels following lung contusion injury and pneumonia. Levels of IL-6, IL-10, hIL-10, KC and MIP-2 were assayed in BAL fluid collected 24 hours after pneumonia inoculation and
    Figure Legend Snippet: Effects of lung-specific IL-10 overexpression on alveolar cytokine/chemokine levels following lung contusion injury and pneumonia. Levels of IL-6, IL-10, hIL-10, KC and MIP-2 were assayed in BAL fluid collected 24 hours after pneumonia inoculation and

    Techniques Used: Over Expression

    Distribution of inflammatory cells in BAL fluid and lung parenchyma following pneumonia and/or lung contusion in IL-10 OE mice. All mice received 5 days of doxycycline chow prior to experimental use. Pneumonia challenged mice were administered 500 CFU’s
    Figure Legend Snippet: Distribution of inflammatory cells in BAL fluid and lung parenchyma following pneumonia and/or lung contusion in IL-10 OE mice. All mice received 5 days of doxycycline chow prior to experimental use. Pneumonia challenged mice were administered 500 CFU’s

    Techniques Used: Mouse Assay

    Effect of lung-specific IL-10 overexpression on survival and bacterial clearance following lung contusion injury and pneumonia. (A) IL-10 OE mice experienced accelerated mortality compared to TG- mice from pneumonia following lung contusion (p
    Figure Legend Snippet: Effect of lung-specific IL-10 overexpression on survival and bacterial clearance following lung contusion injury and pneumonia. (A) IL-10 OE mice experienced accelerated mortality compared to TG- mice from pneumonia following lung contusion (p

    Techniques Used: Over Expression, Mouse Assay

    16) Product Images from "Arsenic trioxide inhibits tumor-induced myeloid-derived suppressor cells and enhances T-cell activity"

    Article Title: Arsenic trioxide inhibits tumor-induced myeloid-derived suppressor cells and enhances T-cell activity

    Journal: Oncology Letters

    doi: 10.3892/ol.2017.5679

    ATO regulates the function of MDSCs through JAK/STAT, PI3K/AKT and MEK/ERK signaling pathway in vitro . (A) The concentration of TNF-α and IL-10 in the supernatant from the MDSCs treated with medium alone (control) or ATO (2 µM) for 120 h was measured by ELISA (*P
    Figure Legend Snippet: ATO regulates the function of MDSCs through JAK/STAT, PI3K/AKT and MEK/ERK signaling pathway in vitro . (A) The concentration of TNF-α and IL-10 in the supernatant from the MDSCs treated with medium alone (control) or ATO (2 µM) for 120 h was measured by ELISA (*P

    Techniques Used: In Vitro, Concentration Assay, Enzyme-linked Immunosorbent Assay

    ATO impairs MDSCs immunosuppressive function. (A) MDSCs isolated from untreated or ATO-treated H22 mice were incubated for 5 days with CFSE-labeled naive T cells (MDSCs: T cell ratio=1:2). Effects of MDSCs on CFSE expression by gated CD4 + T lymphocytes. (B) Proliferation of T lymphocytes co-cultured with MDSCs isolated from untreated or ATO-treated B16 mice was evaluated by determining the cell viability using a CCK-8 assay. (C) The ratio of regulatory T cells (CD4 + CD25 + ) and cytotoxic T lymphocytes (CD8a + ) in the spleen of untreated or ATO-treated B16 mice was analyzed using flow cytometry. (D) The concentration of TNF-α and IL-10 in the serum of B16 mice treated with saline and ATO was measured by ELISA. The results of three independent experiments are shown as the means ± SEM. *P
    Figure Legend Snippet: ATO impairs MDSCs immunosuppressive function. (A) MDSCs isolated from untreated or ATO-treated H22 mice were incubated for 5 days with CFSE-labeled naive T cells (MDSCs: T cell ratio=1:2). Effects of MDSCs on CFSE expression by gated CD4 + T lymphocytes. (B) Proliferation of T lymphocytes co-cultured with MDSCs isolated from untreated or ATO-treated B16 mice was evaluated by determining the cell viability using a CCK-8 assay. (C) The ratio of regulatory T cells (CD4 + CD25 + ) and cytotoxic T lymphocytes (CD8a + ) in the spleen of untreated or ATO-treated B16 mice was analyzed using flow cytometry. (D) The concentration of TNF-α and IL-10 in the serum of B16 mice treated with saline and ATO was measured by ELISA. The results of three independent experiments are shown as the means ± SEM. *P

    Techniques Used: Isolation, Mouse Assay, Incubation, Labeling, Expressing, Cell Culture, CCK-8 Assay, Flow Cytometry, Cytometry, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Related Articles

    Enzyme-linked Immunosorbent Assay:

    Article Title: Lacticaseibacillus casei AMBR2 modulates the epithelial barrier function and immune response in a donor-derived nasal microbiota manner
    Article Snippet: Cytokine secretion Secretion of cytokines IL-8, TNF-α, and IL-10 into the basolateral compartment was measured with an enzyme linked immunosorbent assay (ELISA) at t = 0 h, 24 h, 48, and 72 h after inoculation. .. IL-8 and IL-10 were measured with Human Uncoated ELISA kits (Invitrogen, Merelbeke, Belgium) according to the manufacturer’s instructions. .. TNF-α was measured with an eBioscience Human TNF alpha ELISA Ready-SET-Go!

    Article Title: Novel Therapeutic Approach to Improve Hematopoiesis in low risk MDS by Targeting MDSCs with The Fc-engineered CD33 Antibody BI 836858
    Article Snippet: The relative gene expression was calculated by the ΔΔCt method where untreated cells were the experimental control and the housekeeping gene GAPDH was the internal control. .. Enzyme-linked Immunosorbent Assay (ELISAs) 96-well plates (Nunc-Immuno Plate) were coated with purified monoclonal antibody against either human IL-10 or TGFβ (Pierce-Endogen, Rockford, IL, USA) in 1X PBS, pH 7.4 at room temperature overnight. .. The plates were then incubated in blocking buffer (Pierce-Endogen, Rockford, IL, USA) for 2 h at room temperature and washed in 0.05% Tween-20.

    Article Title: Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus
    Article Snippet: .. Cell culture supernatants were analyzed by ELISA for IL-10 cytokine secretion (Invitrogen). .. Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood samples by Ficoll-Hypaque gradient centrifugation using Histopaque® -1077 (Sigma-Aldrich, St. Louis, MO).

    Multiplex Assay:

    Article Title: Immunity to vector saliva is compromised by short sand fly seasons in endemic regions with temperate climates
    Article Snippet: The results were interpolated from a standard curve using recombinant cytokines and expressed as ratio of cytokine concentration in stimulated/unstimulated cultures. .. Levels of human IFN-γ, IL-10, IL-17, IL-13, IL-5, IL-9, IL-2 and IL-4, or canine IFN-γ, IL-10, IL12p40, TNF-α and IL-6 cytokines were quantified using a multiplex bead-based platform (Life Technologies). .. Flow Cytometry Briefly, non-specific FC binding sites on viable PBMC were blocked for 10 min at 4 °C, using anti-CD16/32 FcγR antibody (BD).

    Construct:

    Article Title: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct
    Article Snippet: Construction of the EBV and HCMV IL-10 expression vectors Molecular methods were performed as described by Sambrook et al. [ ]. .. To construct plasmids pGA4 and pGA6, 700 and 664 bp DNA fragments including the T7 promoter and the first 66 nucleotides of the E. coli ompF gene (NC_000913.2) encoding the signal peptide sequence fused in frame to E. coli codon optimized genes of the mature form of either HCMV IL-10 (1LQS_M) or EBV IL-10 (YP_401634) were de novo synthesized and cloned into Sac I/Kpn I digested pUC-derived vector pMA by GeneArt. .. The pGA4 and pGA6 plasmids were Eco RI digested and the resulting DNA fragments including the vIL-10 transporter construct and the T7 promoter region were subsequently ligated into Eco RI digested pUC19 resulting in the expression plasmids pAZ1c and pAZ1e.

    Sequencing:

    Article Title: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct
    Article Snippet: Construction of the EBV and HCMV IL-10 expression vectors Molecular methods were performed as described by Sambrook et al. [ ]. .. To construct plasmids pGA4 and pGA6, 700 and 664 bp DNA fragments including the T7 promoter and the first 66 nucleotides of the E. coli ompF gene (NC_000913.2) encoding the signal peptide sequence fused in frame to E. coli codon optimized genes of the mature form of either HCMV IL-10 (1LQS_M) or EBV IL-10 (YP_401634) were de novo synthesized and cloned into Sac I/Kpn I digested pUC-derived vector pMA by GeneArt. .. The pGA4 and pGA6 plasmids were Eco RI digested and the resulting DNA fragments including the vIL-10 transporter construct and the T7 promoter region were subsequently ligated into Eco RI digested pUC19 resulting in the expression plasmids pAZ1c and pAZ1e.

    Synthesized:

    Article Title: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct
    Article Snippet: Construction of the EBV and HCMV IL-10 expression vectors Molecular methods were performed as described by Sambrook et al. [ ]. .. To construct plasmids pGA4 and pGA6, 700 and 664 bp DNA fragments including the T7 promoter and the first 66 nucleotides of the E. coli ompF gene (NC_000913.2) encoding the signal peptide sequence fused in frame to E. coli codon optimized genes of the mature form of either HCMV IL-10 (1LQS_M) or EBV IL-10 (YP_401634) were de novo synthesized and cloned into Sac I/Kpn I digested pUC-derived vector pMA by GeneArt. .. The pGA4 and pGA6 plasmids were Eco RI digested and the resulting DNA fragments including the vIL-10 transporter construct and the T7 promoter region were subsequently ligated into Eco RI digested pUC19 resulting in the expression plasmids pAZ1c and pAZ1e.

    Clone Assay:

    Article Title: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct
    Article Snippet: Construction of the EBV and HCMV IL-10 expression vectors Molecular methods were performed as described by Sambrook et al. [ ]. .. To construct plasmids pGA4 and pGA6, 700 and 664 bp DNA fragments including the T7 promoter and the first 66 nucleotides of the E. coli ompF gene (NC_000913.2) encoding the signal peptide sequence fused in frame to E. coli codon optimized genes of the mature form of either HCMV IL-10 (1LQS_M) or EBV IL-10 (YP_401634) were de novo synthesized and cloned into Sac I/Kpn I digested pUC-derived vector pMA by GeneArt. .. The pGA4 and pGA6 plasmids were Eco RI digested and the resulting DNA fragments including the vIL-10 transporter construct and the T7 promoter region were subsequently ligated into Eco RI digested pUC19 resulting in the expression plasmids pAZ1c and pAZ1e.

    Article Title: IKZF3/Aiolos Is Associated with but Not Sufficient for the Expression of IL-10 by CD4+ T Cells
    Article Snippet: Cells were rested overnight at a density of 1 × 105 cells/ml, then stained for IL-10, IL-17A, IFN-γ, and IKZF3. .. Plasmids and cloning The selected regions of the human IL10 locus (indicated in ) were amplified by PCR using the BAC RP11-262N9 (Thermo Fisher Scientific) as a template, and TOPO-cloned into pCR-Blunt II-TOPO (Invitrogen). .. These were then sequenced to confirm 100% conformity to the reference sequence.

    Plasmid Preparation:

    Article Title: Secretory expression of biologically active human Herpes virus interleukin-10 analogues in Escherichia coli via a modified Sec-dependent transporter construct
    Article Snippet: Construction of the EBV and HCMV IL-10 expression vectors Molecular methods were performed as described by Sambrook et al. [ ]. .. To construct plasmids pGA4 and pGA6, 700 and 664 bp DNA fragments including the T7 promoter and the first 66 nucleotides of the E. coli ompF gene (NC_000913.2) encoding the signal peptide sequence fused in frame to E. coli codon optimized genes of the mature form of either HCMV IL-10 (1LQS_M) or EBV IL-10 (YP_401634) were de novo synthesized and cloned into Sac I/Kpn I digested pUC-derived vector pMA by GeneArt. .. The pGA4 and pGA6 plasmids were Eco RI digested and the resulting DNA fragments including the vIL-10 transporter construct and the T7 promoter region were subsequently ligated into Eco RI digested pUC19 resulting in the expression plasmids pAZ1c and pAZ1e.

    Purification:

    Article Title: Novel Therapeutic Approach to Improve Hematopoiesis in low risk MDS by Targeting MDSCs with The Fc-engineered CD33 Antibody BI 836858
    Article Snippet: The relative gene expression was calculated by the ΔΔCt method where untreated cells were the experimental control and the housekeeping gene GAPDH was the internal control. .. Enzyme-linked Immunosorbent Assay (ELISAs) 96-well plates (Nunc-Immuno Plate) were coated with purified monoclonal antibody against either human IL-10 or TGFβ (Pierce-Endogen, Rockford, IL, USA) in 1X PBS, pH 7.4 at room temperature overnight. .. The plates were then incubated in blocking buffer (Pierce-Endogen, Rockford, IL, USA) for 2 h at room temperature and washed in 0.05% Tween-20.

    Staining:

    Article Title: Mesenchymal stem cells prevent overwhelming inflammation and reduce infection severity via recruiting CXCR3+ regulatory T cells
    Article Snippet: The following anti‐mouse mAbs purchased from Becton, Dickinson and Company (BD, New Jersey, United States), BioLegend or eBioscience were used: CD45 (30‐F11), CD11c (N418), F4/80 (BM8), CD4 (RM4‐5), CD25 (PC61.5), CXCR3 (CXCR3‐173), CXCR5 (SPRCL5), CCR2 (SA203G11), CCR4 (2G12), CCR5(HM‐CCR5), Foxp3 (FJK‐16s), T‐bet (eBio4B10), Ki67 (SolA15), LAG3 (C9B7W), ICOS (15F9), CTLA (UC10‐4B9), LAP (TW7‐16B4) and IL‐10 (JES5‐16E3). .. For the intracellular staining of Foxp3, T‐bet, Ki67 and IL‐10, cells were surface‐stained with CD4 and followed by permeabilisation with Foxp3 staining kit (eBiosciences). .. Then, the cells were stained with Foxp3, Ki67, IL‐10 and T‐bet.

    Amplification:

    Article Title: IKZF3/Aiolos Is Associated with but Not Sufficient for the Expression of IL-10 by CD4+ T Cells
    Article Snippet: Cells were rested overnight at a density of 1 × 105 cells/ml, then stained for IL-10, IL-17A, IFN-γ, and IKZF3. .. Plasmids and cloning The selected regions of the human IL10 locus (indicated in ) were amplified by PCR using the BAC RP11-262N9 (Thermo Fisher Scientific) as a template, and TOPO-cloned into pCR-Blunt II-TOPO (Invitrogen). .. These were then sequenced to confirm 100% conformity to the reference sequence.

    Polymerase Chain Reaction:

    Article Title: IKZF3/Aiolos Is Associated with but Not Sufficient for the Expression of IL-10 by CD4+ T Cells
    Article Snippet: Cells were rested overnight at a density of 1 × 105 cells/ml, then stained for IL-10, IL-17A, IFN-γ, and IKZF3. .. Plasmids and cloning The selected regions of the human IL10 locus (indicated in ) were amplified by PCR using the BAC RP11-262N9 (Thermo Fisher Scientific) as a template, and TOPO-cloned into pCR-Blunt II-TOPO (Invitrogen). .. These were then sequenced to confirm 100% conformity to the reference sequence.

    BAC Assay:

    Article Title: IKZF3/Aiolos Is Associated with but Not Sufficient for the Expression of IL-10 by CD4+ T Cells
    Article Snippet: Cells were rested overnight at a density of 1 × 105 cells/ml, then stained for IL-10, IL-17A, IFN-γ, and IKZF3. .. Plasmids and cloning The selected regions of the human IL10 locus (indicated in ) were amplified by PCR using the BAC RP11-262N9 (Thermo Fisher Scientific) as a template, and TOPO-cloned into pCR-Blunt II-TOPO (Invitrogen). .. These were then sequenced to confirm 100% conformity to the reference sequence.

    Cell Culture:

    Article Title: Peptide nanofiber hydrogel adjuvanted live virus vaccine enhances cross-protective immunity to porcine reproductive and respiratory syndrome virus
    Article Snippet: .. Cell culture supernatants were analyzed by ELISA for IL-10 cytokine secretion (Invitrogen). .. Peripheral blood mononuclear cells (PBMCs) were isolated from heparinized blood samples by Ficoll-Hypaque gradient centrifugation using Histopaque® -1077 (Sigma-Aldrich, St. Louis, MO).

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    <t>Cytokine</t> production by DCs infected with ES
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    Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, <t>anti-IL-4</t> and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P
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    Rebamipide reciprocally regulates Th17/Treg cell imbalance. Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. a The tissues were stained with specific Abs against CD4 ( green ), CD25 ( white ), IL-17 ( red ), FOXP3 ( green ), p -STAT3–Y705 ( red ), and p -STAT3–S727 ( red ). Positive cells are shown in the bar graphs . b Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. Splenocytes were stained with Abs against CD4–PerCP, IL-17–PE, and <t>IL-10–FITC</t> to determine the presence of Th17 and IL-10-expressing Treg cells. Data are expressed as the mean ± SEM of 3 independent experiments (* P
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    Cytokine production by DCs infected with ES

    Journal: Journal of immunology (Baltimore, Md. : 1950)

    Article Title: Enterobacter sakazakii targets DC-SIGN to induce immunosuppressive responses in dendritic cells by modulating MAP kinases

    doi: 10.4049/jimmunol.0902029

    Figure Lengend Snippet: Cytokine production by DCs infected with ES

    Article Snippet: Cytokine (TNF-α, IL-1β, IL-6, IL-12 p70, IL-10 and TGF-β) production in cell culture supernatants of DC-bacteria co-culture experiments collected after 24 and 48 h of incubation was carried out using Biosource ELISA kits (Invitrogen, Carlsbad, CA) according to the manufacturer’s instructions.

    Techniques: Infection

    Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, anti-IL-4 and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P

    Journal: Frontiers in Cellular and Infection Microbiology

    Article Title: Coxsackievirus B3 Directly Induced Th17 Cell Differentiation by Inhibiting Nup98 Expression in Patients with Acute Viral Myocarditis

    doi: 10.3389/fcimb.2016.00171

    Figure Lengend Snippet: Effects of Nup98 on Th17 cell differentiation in AVMC . CVB3 infected CD4 + T cells from peripheral blood in AVMC patients were transfected with Nup98 siRNA or Nup98 cDNA by the Human T Cell Nucleofector Kit and cultured with anti-CD3, anti-CD28, anti-IL-4 and anti-IFN-γ for 72 h. (A) Representative pictures of Th17 cell frequencies of CD4 + T cell in pcDNA3.1, NC, pcDNA3.1-Nup98, and siRNA-nup98 groups were listed. (B) The statistical analysis for Th17 frequencies was shown in histogram. (C) The GFP-transfection efficiency was detected by flow cytometry and a typical FACS picture was shown. (D) The mRNA levels of Nup98, RORγT and IL-17 in CD4 + T cells of four groups. (E) The cultural supernatant IL-17 concentration was measured by ELISA. Data are shown as mean ± SEM. * P

    Article Snippet: After washing, cells were cultured with 1640 medium containing 5% FBS, 5 μg/mL of anti-CD3 (ebioscience), 2 μg/mL soluble anti-CD28 (eBioscience), 10 μg/mL anti-IL-4 (ebioscience), and 10 μg/mL anti-IFN-γ (ebioscience) for 5 days at room temperature.

    Techniques: Cell Differentiation, Infection, Transfection, Cell Culture, Flow Cytometry, Cytometry, FACS, Concentration Assay, Enzyme-linked Immunosorbent Assay

    Rebamipide reciprocally regulates Th17/Treg cell imbalance. Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. a The tissues were stained with specific Abs against CD4 ( green ), CD25 ( white ), IL-17 ( red ), FOXP3 ( green ), p -STAT3–Y705 ( red ), and p -STAT3–S727 ( red ). Positive cells are shown in the bar graphs . b Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. Splenocytes were stained with Abs against CD4–PerCP, IL-17–PE, and IL-10–FITC to determine the presence of Th17 and IL-10-expressing Treg cells. Data are expressed as the mean ± SEM of 3 independent experiments (* P

    Journal: Journal of Translational Medicine

    Article Title: Rebamipide prevents peripheral arthritis and intestinal inflammation by reciprocally regulating Th17/Treg cell imbalance in mice with curdlan-induced spondyloarthritis

    doi: 10.1186/s12967-016-0942-5

    Figure Lengend Snippet: Rebamipide reciprocally regulates Th17/Treg cell imbalance. Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. a The tissues were stained with specific Abs against CD4 ( green ), CD25 ( white ), IL-17 ( red ), FOXP3 ( green ), p -STAT3–Y705 ( red ), and p -STAT3–S727 ( red ). Positive cells are shown in the bar graphs . b Spleen tissues were isolated from mice in each group at 14 weeks after curdlan injection. Splenocytes were stained with Abs against CD4–PerCP, IL-17–PE, and IL-10–FITC to determine the presence of Th17 and IL-10-expressing Treg cells. Data are expressed as the mean ± SEM of 3 independent experiments (* P

    Article Snippet: Cells were permeabilized and fixed with CytoFix (BD Biosciences, San Jose, CA, USA), as instructed by the manufacturer, and were stained with Abs against IL-17–PE and IL-10–FITC (eBioscience).

    Techniques: Isolation, Mouse Assay, Injection, Staining, Expressing

    IL-10 inhibits FcεRI-dependent TNF-α expression by blood mDCs

    Journal:

    Article Title: Interferons Modulate Fc?RI-dependent Production of Autoregulatory IL-10 by Circulating Human Monocytoid Dendritic Cells

    doi: 10.1016/j.jaci.2008.09.013

    Figure Lengend Snippet: IL-10 inhibits FcεRI-dependent TNF-α expression by blood mDCs

    Article Snippet: FcεRI-mediated IL-10 neutralization experiments were performed by adding anti-IL-10 Ab (500 ng/mL, clone JESS-9D7, eBioscience) simultaneously with the AER-37 anti-FcεRIα Ab from eBioscience (1:167 of the stock or ~75 ng/mL) for 16 h.

    Techniques: Expressing

    TLR9-induced IFN-α from pDCs enhances FcεRI-dependent IL-10 secretion by mDCs

    Journal:

    Article Title: Interferons Modulate Fc?RI-dependent Production of Autoregulatory IL-10 by Circulating Human Monocytoid Dendritic Cells

    doi: 10.1016/j.jaci.2008.09.013

    Figure Lengend Snippet: TLR9-induced IFN-α from pDCs enhances FcεRI-dependent IL-10 secretion by mDCs

    Article Snippet: FcεRI-mediated IL-10 neutralization experiments were performed by adding anti-IL-10 Ab (500 ng/mL, clone JESS-9D7, eBioscience) simultaneously with the AER-37 anti-FcεRIα Ab from eBioscience (1:167 of the stock or ~75 ng/mL) for 16 h.

    Techniques:

    Kinetics for FcεRI-dependent TNF-α and IL-10 secretion by blood mDCs

    Journal:

    Article Title: Interferons Modulate Fc?RI-dependent Production of Autoregulatory IL-10 by Circulating Human Monocytoid Dendritic Cells

    doi: 10.1016/j.jaci.2008.09.013

    Figure Lengend Snippet: Kinetics for FcεRI-dependent TNF-α and IL-10 secretion by blood mDCs

    Article Snippet: FcεRI-mediated IL-10 neutralization experiments were performed by adding anti-IL-10 Ab (500 ng/mL, clone JESS-9D7, eBioscience) simultaneously with the AER-37 anti-FcεRIα Ab from eBioscience (1:167 of the stock or ~75 ng/mL) for 16 h.

    Techniques:

    Inhibitory effects of IL-10 on FcεRI-dependent responses in mDCs are not from a down-regulation in FcεRIα and co-stimulatory molecule expression

    Journal:

    Article Title: Interferons Modulate Fc?RI-dependent Production of Autoregulatory IL-10 by Circulating Human Monocytoid Dendritic Cells

    doi: 10.1016/j.jaci.2008.09.013

    Figure Lengend Snippet: Inhibitory effects of IL-10 on FcεRI-dependent responses in mDCs are not from a down-regulation in FcεRIα and co-stimulatory molecule expression

    Article Snippet: FcεRI-mediated IL-10 neutralization experiments were performed by adding anti-IL-10 Ab (500 ng/mL, clone JESS-9D7, eBioscience) simultaneously with the AER-37 anti-FcεRIα Ab from eBioscience (1:167 of the stock or ~75 ng/mL) for 16 h.

    Techniques: Expressing

    Interferons modulate FcεRI-dependent IL-10 secretion by mDCs

    Journal:

    Article Title: Interferons Modulate Fc?RI-dependent Production of Autoregulatory IL-10 by Circulating Human Monocytoid Dendritic Cells

    doi: 10.1016/j.jaci.2008.09.013

    Figure Lengend Snippet: Interferons modulate FcεRI-dependent IL-10 secretion by mDCs

    Article Snippet: FcεRI-mediated IL-10 neutralization experiments were performed by adding anti-IL-10 Ab (500 ng/mL, clone JESS-9D7, eBioscience) simultaneously with the AER-37 anti-FcεRIα Ab from eBioscience (1:167 of the stock or ~75 ng/mL) for 16 h.

    Techniques: