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Characterization of SERT in <t>JAR</t> cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% <t>FBS</t> with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate
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1) Product Images from "At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner"

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

Journal:

doi: 10.1111/j.1471-4159.2007.04469.x

Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate
Figure Legend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

Techniques Used: Cell Culture

Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).
Figure Legend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

Techniques Used: Concentration Assay, Cell Culture

2) Product Images from "At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner"

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

Journal:

doi: 10.1111/j.1471-4159.2007.04469.x

Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate
Figure Legend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

Techniques Used: Cell Culture

Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).
Figure Legend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

Techniques Used: Concentration Assay, Cell Culture

3) Product Images from "At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner"

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

Journal:

doi: 10.1111/j.1471-4159.2007.04469.x

Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate
Figure Legend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

Techniques Used: Cell Culture

Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).
Figure Legend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

Techniques Used: Concentration Assay, Cell Culture

4) Product Images from "At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner"

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

Journal:

doi: 10.1111/j.1471-4159.2007.04469.x

Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate
Figure Legend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

Techniques Used: Cell Culture

Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).
Figure Legend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

Techniques Used: Concentration Assay, Cell Culture

5) Product Images from "At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner"

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

Journal:

doi: 10.1111/j.1471-4159.2007.04469.x

Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate
Figure Legend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

Techniques Used: Cell Culture

Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).
Figure Legend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

Techniques Used: Concentration Assay, Cell Culture

6) Product Images from "At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner"

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

Journal:

doi: 10.1111/j.1471-4159.2007.04469.x

Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate
Figure Legend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

Techniques Used: Cell Culture

Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).
Figure Legend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

Techniques Used: Concentration Assay, Cell Culture

7) Product Images from "At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner"

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

Journal:

doi: 10.1111/j.1471-4159.2007.04469.x

Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate
Figure Legend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

Techniques Used: Cell Culture

Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).
Figure Legend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

Techniques Used: Concentration Assay, Cell Culture

Related Articles

other:

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: The 5HT uptake rates were monitored in JAR cells which entered the cell cycle in insulin-free FBS containing full RPMI media supplemented with 5.5 mmol/L glucose ( ).

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: Therefore, our next step was to evaluate the impact of 25 mmol/L D-glucose on the self-association of placental SERT molecules in full RPMI medium supplemented with insulin-free FBS.

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: JAR cells were seeded in full RPMI supplemented with insulin-free FBS.

DNA Synthesis:

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: The rate of DNA synthesis confirmed that quiescent JAR cells enter S- and G2-phase after 8 and 16 h, respectively, during incubation in insulin-free full media supplemented with or without 25 mmol/L glucose (Panel B). .. Also, these data demonstrate that there is a 60% increase in overall DNA synthesis of JAR cells if they enter the cell cycle in media supplemented with 25 mmol/L D-glucose and insulin-free FBS ( ). ..

Cell Culture:

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: A diabetic condition is defined by a blood glucose concentration greater than 250 mg/dL (13.8 mmol/L). .. Thus, the 5HT uptake rate of SERT was measured in JAR cells cultured in RPMI medium supplemented with insulin-free FBS (Gemini Bio-Products) and either 5.5 mmol/L (physiologic concentration) or 25 mmol/L (diabetic-like concentration) D-glucose ( ; ). .. To measure the whole-cell surface expression and the self-association ability of the transporter, these cells were transiently transfected with rSERT constructs using a 1: 3 ratio of Lipofectamine 2000 reagent in opti-MEM I (Invitrogen, Carlsbad, CA, USA).

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: .. To examine the possibility that the exogenous 5.5 mmol/L D-glucose (normoglycemia) has a time-dependent effect on the placental 5HT system, JAR cells were cultured in RPMI media supplemented with insulin-free FBS. .. However, even after 96 h incubation, the 5HT uptake rates of these cells were not altered by D-glucose at physiological concentration in the absence of insulin ( ).

Concentration Assay:

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: A diabetic condition is defined by a blood glucose concentration greater than 250 mg/dL (13.8 mmol/L). .. Thus, the 5HT uptake rate of SERT was measured in JAR cells cultured in RPMI medium supplemented with insulin-free FBS (Gemini Bio-Products) and either 5.5 mmol/L (physiologic concentration) or 25 mmol/L (diabetic-like concentration) D-glucose ( ; ). .. To measure the whole-cell surface expression and the self-association ability of the transporter, these cells were transiently transfected with rSERT constructs using a 1: 3 ratio of Lipofectamine 2000 reagent in opti-MEM I (Invitrogen, Carlsbad, CA, USA).

Incubation:

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: The SERT mRNA level expression was measured in quiescent JAR cells ( ). .. Then, the quiescent cells were incubated in insulin-free FBS containing full media supplemented with 5.5 or 25 mmol/L glucose. .. Their SERT mRNA was extracted, and RT-PCR was performed ( ).

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: On the following day, cells were transfected with myc-SERT/Flag-SERT (1: 1 ratio) or only one of the constructs. .. Following a 7-h incubation, the transfection media was changed to full RPMI containing insulin-free FBS and 5.5, 15, or 25 mmol/L D-glucose ( , lanes 3, 7 and 5, respectively). .. Cells were lysed and immunoprecipitation was performed with polyclonal Flag-antibody, as described in the Methods section ( ).

Transfection:

Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner
Article Snippet: On the following day, cells were transfected with myc-SERT/Flag-SERT (1: 1 ratio) or only one of the constructs. .. Following a 7-h incubation, the transfection media was changed to full RPMI containing insulin-free FBS and 5.5, 15, or 25 mmol/L D-glucose ( , lanes 3, 7 and 5, respectively). .. Cells were lysed and immunoprecipitation was performed with polyclonal Flag-antibody, as described in the Methods section ( ).

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    Gemini Bio insulin free fbs
    Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% <t>FBS</t> with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 <t>mmol/L.</t> The [ 3 H]-5HT uptake rate
    Insulin Free Fbs, supplied by Gemini Bio, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/insulin free fbs/product/Gemini Bio
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    insulin free fbs - by Bioz Stars, 2021-04
    86/100 stars
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    Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

    Journal:

    Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

    doi: 10.1111/j.1471-4159.2007.04469.x

    Figure Lengend Snippet: Characterization of SERT in JAR cells with glucose at different concentrations. (a) JAR cells were cultured in RPMI supplemented with 10% FBS with/without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L. The [ 3 H]-5HT uptake rate

    Article Snippet: Also, these data demonstrate that there is a 60% increase in overall DNA synthesis of JAR cells if they enter the cell cycle in media supplemented with 25 mmol/L D-glucose and insulin-free FBS ( ).

    Techniques: Cell Culture

    Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

    Journal:

    Article Title: At diabetes-like concentration, glucose down-regulates the placental serotonin transport system in a cell-cycle-dependent manner

    doi: 10.1111/j.1471-4159.2007.04469.x

    Figure Lengend Snippet: Concentration-dependent effect of exogenous D-glucose on relative SERT mRNA in JAR cells. JAR cells were cultured in RPMI supplemented with 10% FBS without insulin and D-glucose at different concentrations, 5.5, 10, 15, 25 mmol/L (lanes 2–5).

    Article Snippet: Also, these data demonstrate that there is a 60% increase in overall DNA synthesis of JAR cells if they enter the cell cycle in media supplemented with 25 mmol/L D-glucose and insulin-free FBS ( ).

    Techniques: Concentration Assay, Cell Culture