input bacmid dna  (New England Biolabs)


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    New England Biolabs input bacmid dna
    Subcellular localization of total actin and F-actin in Sf9 cells transfected with different recombinant viruses. (A) Total actin localization. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO <t>bacmid</t> <t>DNA.</t> At 36 h p.t., the cells were fixed,
    Input Bacmid Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/input bacmid dna/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    input bacmid dna - by Bioz Stars, 2020-08
    90/100 stars

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    Images

    1) Product Images from "The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly"

    Article Title: The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly

    Journal: Journal of Virology

    doi: 10.1128/JVI.02885-15

    Subcellular localization of total actin and F-actin in Sf9 cells transfected with different recombinant viruses. (A) Total actin localization. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA. At 36 h p.t., the cells were fixed,
    Figure Legend Snippet: Subcellular localization of total actin and F-actin in Sf9 cells transfected with different recombinant viruses. (A) Total actin localization. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA. At 36 h p.t., the cells were fixed,

    Techniques Used: Transfection, Recombinant

    Subcellular localization of the minor capsid proteins PP78/83, BV/ODV-C42 (C42), and 38K, as indicated. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA and fixed at 36 h p.t. (A) Transfected cells were incubated with antisera
    Figure Legend Snippet: Subcellular localization of the minor capsid proteins PP78/83, BV/ODV-C42 (C42), and 38K, as indicated. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA and fixed at 36 h p.t. (A) Transfected cells were incubated with antisera

    Techniques Used: Transfection, Incubation

    Subcellular localization of the major capsid protein VP39. Sf9 cells were transfected with vAc54KO (A), vAc54:2HA (B), or v38KKO (C) bacmid DNA. Transfected cells were fixed at 36 h p.t. and incubated with antisera against VP39 (rabbit) and IE-1 (mouse)
    Figure Legend Snippet: Subcellular localization of the major capsid protein VP39. Sf9 cells were transfected with vAc54KO (A), vAc54:2HA (B), or v38KKO (C) bacmid DNA. Transfected cells were fixed at 36 h p.t. and incubated with antisera against VP39 (rabbit) and IE-1 (mouse)

    Techniques Used: Transfection, Incubation

    Characterization of the ac54 -knockout bacmid. (A) Strategy for the construction of the recombinant viruses vAc54KO, vAc54:2HA, and vAcWT. (B) Sf9 cells were transfected with vAc54KO, vAc54:2HA, and vAcWT bacmid DNA and observed at 72 h p.t. (C) Virus
    Figure Legend Snippet: Characterization of the ac54 -knockout bacmid. (A) Strategy for the construction of the recombinant viruses vAc54KO, vAc54:2HA, and vAcWT. (B) Sf9 cells were transfected with vAc54KO, vAc54:2HA, and vAcWT bacmid DNA and observed at 72 h p.t. (C) Virus

    Techniques Used: Knock-Out, Recombinant, Transfection

    Immunoelectron microscopy analysis of Sf9 cells transfected with vAc54KO or vAcWT bacmid DNA at 72 h p.t. using antisera against different nucleocapsid structural proteins. For the experiments shown in panels A to C, Sf9 cells were transfected with vAc54KO
    Figure Legend Snippet: Immunoelectron microscopy analysis of Sf9 cells transfected with vAc54KO or vAcWT bacmid DNA at 72 h p.t. using antisera against different nucleocapsid structural proteins. For the experiments shown in panels A to C, Sf9 cells were transfected with vAc54KO

    Techniques Used: Immuno-Electron Microscopy, Transfection

    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly
    Article Snippet: .. To eliminate input bacmid DNA, 1 μg of DNA was digested with 20 units of the DpnI restriction enzyme (NEB) overnight in a 40-μl reaction volume. qPCR was performed using Hot Start PCR master mix III (Chaoshi-Bio) and primers targeting a 100-bp region of the gp41 gene under conditions described previously ( ). .. Sf9 cells (5 × 105 ) were seeded into 35-mm glass-bottom culture dishes (MatTek) and incubated at 27°C for 12 h, followed by transfection with 2 μg of bacmid DNA.

    Hot Start PCR:

    Article Title: The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly
    Article Snippet: .. To eliminate input bacmid DNA, 1 μg of DNA was digested with 20 units of the DpnI restriction enzyme (NEB) overnight in a 40-μl reaction volume. qPCR was performed using Hot Start PCR master mix III (Chaoshi-Bio) and primers targeting a 100-bp region of the gp41 gene under conditions described previously ( ). .. Sf9 cells (5 × 105 ) were seeded into 35-mm glass-bottom culture dishes (MatTek) and incubated at 27°C for 12 h, followed by transfection with 2 μg of bacmid DNA.

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    New England Biolabs input bacmid dna
    Subcellular localization of total actin and F-actin in Sf9 cells transfected with different recombinant viruses. (A) Total actin localization. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO <t>bacmid</t> <t>DNA.</t> At 36 h p.t., the cells were fixed,
    Input Bacmid Dna, supplied by New England Biolabs, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/input bacmid dna/product/New England Biolabs
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    input bacmid dna - by Bioz Stars, 2020-08
    90/100 stars
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    Subcellular localization of total actin and F-actin in Sf9 cells transfected with different recombinant viruses. (A) Total actin localization. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA. At 36 h p.t., the cells were fixed,

    Journal: Journal of Virology

    Article Title: The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly

    doi: 10.1128/JVI.02885-15

    Figure Lengend Snippet: Subcellular localization of total actin and F-actin in Sf9 cells transfected with different recombinant viruses. (A) Total actin localization. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA. At 36 h p.t., the cells were fixed,

    Article Snippet: To eliminate input bacmid DNA, 1 μg of DNA was digested with 20 units of the DpnI restriction enzyme (NEB) overnight in a 40-μl reaction volume. qPCR was performed using Hot Start PCR master mix III (Chaoshi-Bio) and primers targeting a 100-bp region of the gp41 gene under conditions described previously ( ).

    Techniques: Transfection, Recombinant

    Subcellular localization of the minor capsid proteins PP78/83, BV/ODV-C42 (C42), and 38K, as indicated. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA and fixed at 36 h p.t. (A) Transfected cells were incubated with antisera

    Journal: Journal of Virology

    Article Title: The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly

    doi: 10.1128/JVI.02885-15

    Figure Lengend Snippet: Subcellular localization of the minor capsid proteins PP78/83, BV/ODV-C42 (C42), and 38K, as indicated. Sf9 cells were transfected with vAc54KO, vAc54:2HA, or v38KKO bacmid DNA and fixed at 36 h p.t. (A) Transfected cells were incubated with antisera

    Article Snippet: To eliminate input bacmid DNA, 1 μg of DNA was digested with 20 units of the DpnI restriction enzyme (NEB) overnight in a 40-μl reaction volume. qPCR was performed using Hot Start PCR master mix III (Chaoshi-Bio) and primers targeting a 100-bp region of the gp41 gene under conditions described previously ( ).

    Techniques: Transfection, Incubation

    Subcellular localization of the major capsid protein VP39. Sf9 cells were transfected with vAc54KO (A), vAc54:2HA (B), or v38KKO (C) bacmid DNA. Transfected cells were fixed at 36 h p.t. and incubated with antisera against VP39 (rabbit) and IE-1 (mouse)

    Journal: Journal of Virology

    Article Title: The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly

    doi: 10.1128/JVI.02885-15

    Figure Lengend Snippet: Subcellular localization of the major capsid protein VP39. Sf9 cells were transfected with vAc54KO (A), vAc54:2HA (B), or v38KKO (C) bacmid DNA. Transfected cells were fixed at 36 h p.t. and incubated with antisera against VP39 (rabbit) and IE-1 (mouse)

    Article Snippet: To eliminate input bacmid DNA, 1 μg of DNA was digested with 20 units of the DpnI restriction enzyme (NEB) overnight in a 40-μl reaction volume. qPCR was performed using Hot Start PCR master mix III (Chaoshi-Bio) and primers targeting a 100-bp region of the gp41 gene under conditions described previously ( ).

    Techniques: Transfection, Incubation

    Characterization of the ac54 -knockout bacmid. (A) Strategy for the construction of the recombinant viruses vAc54KO, vAc54:2HA, and vAcWT. (B) Sf9 cells were transfected with vAc54KO, vAc54:2HA, and vAcWT bacmid DNA and observed at 72 h p.t. (C) Virus

    Journal: Journal of Virology

    Article Title: The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly

    doi: 10.1128/JVI.02885-15

    Figure Lengend Snippet: Characterization of the ac54 -knockout bacmid. (A) Strategy for the construction of the recombinant viruses vAc54KO, vAc54:2HA, and vAcWT. (B) Sf9 cells were transfected with vAc54KO, vAc54:2HA, and vAcWT bacmid DNA and observed at 72 h p.t. (C) Virus

    Article Snippet: To eliminate input bacmid DNA, 1 μg of DNA was digested with 20 units of the DpnI restriction enzyme (NEB) overnight in a 40-μl reaction volume. qPCR was performed using Hot Start PCR master mix III (Chaoshi-Bio) and primers targeting a 100-bp region of the gp41 gene under conditions described previously ( ).

    Techniques: Knock-Out, Recombinant, Transfection

    Immunoelectron microscopy analysis of Sf9 cells transfected with vAc54KO or vAcWT bacmid DNA at 72 h p.t. using antisera against different nucleocapsid structural proteins. For the experiments shown in panels A to C, Sf9 cells were transfected with vAc54KO

    Journal: Journal of Virology

    Article Title: The Autographa californica Multiple Nucleopolyhedrovirus ac54 Gene Is Crucial for Localization of the Major Capsid Protein VP39 at the Site of Nucleocapsid Assembly

    doi: 10.1128/JVI.02885-15

    Figure Lengend Snippet: Immunoelectron microscopy analysis of Sf9 cells transfected with vAc54KO or vAcWT bacmid DNA at 72 h p.t. using antisera against different nucleocapsid structural proteins. For the experiments shown in panels A to C, Sf9 cells were transfected with vAc54KO

    Article Snippet: To eliminate input bacmid DNA, 1 μg of DNA was digested with 20 units of the DpnI restriction enzyme (NEB) overnight in a 40-μl reaction volume. qPCR was performed using Hot Start PCR master mix III (Chaoshi-Bio) and primers targeting a 100-bp region of the gp41 gene under conditions described previously ( ).

    Techniques: Immuno-Electron Microscopy, Transfection