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TaKaRa infusion hd kits
Infusion Hd Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/infusion hd kits/product/TaKaRa
Average 94 stars, based on 3 article reviews
Price from $9.99 to $1999.99
infusion hd kits - by Bioz Stars, 2020-04
94/100 stars

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Molecular Cloning:

Article Title: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Article Snippet: Paragraph title: Molecular cloning and mutagenesis ... Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech).

Amplification:

Article Title: The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae
Article Snippet: Briefly, 788 bp upstream of the PGK1 gene were amplified from the pHVX2 vector , and cloned into the Sal1 site of pUG6. .. InFusion HD kits (Clontech) were used for cloning.

Clone Assay:

Article Title: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Article Snippet: Synthetic DNA oligonucleotides used for cloning were purchased from Integrated DNA Technologies. .. Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech).

Article Title: The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae
Article Snippet: .. InFusion HD kits (Clontech) were used for cloning. .. The resulting plasmid was used as a template to generate the amplicons containing the PGK1 promoter linked the kanMX4 selection cassette.

Mutagenesis:

Article Title: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Article Snippet: Paragraph title: Molecular cloning and mutagenesis ... Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech).

Article Title: The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae
Article Snippet: The haploid colonies, which spontaneously revert to diploid, were selected for antibiotic resistance, thus generating strains that were homozygous for the intended mutation. .. InFusion HD kits (Clontech) were used for cloning.

Isolation:

Article Title: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Article Snippet: Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech). .. Small-scale isolation of plasmid DNA was performed with Mini-Prep kits (Qiagen); large-scale DNA plasmid purification was done with GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kits (Sigma-Aldrich).

Synthesized:

Article Title: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Article Snippet: The Rp BphP1/PAS-GAF domains and QuasAr2-ER2 genes were synthesized de novo (Genscript) with mammalian codon optimization and subcloned into the pN1 vector (Clontech) using Age I/ Not I sites. .. Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech).

Construct:

Article Title: The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae
Article Snippet: In order to generate strains overexpressing our genes of interest, the pCW1 plasmid containing the PGK1 promoter was constructed. .. InFusion HD kits (Clontech) were used for cloning.

Purification:

Article Title: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Article Snippet: Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech). .. Small-scale isolation of plasmid DNA was performed with Mini-Prep kits (Qiagen); large-scale DNA plasmid purification was done with GenElute™ HP Endotoxin-Free Plasmid Maxiprep Kits (Sigma-Aldrich).

Selection:

Article Title: The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae
Article Snippet: InFusion HD kits (Clontech) were used for cloning. .. The resulting plasmid was used as a template to generate the amplicons containing the PGK1 promoter linked the kanMX4 selection cassette.

Polymerase Chain Reaction:

Article Title: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Article Snippet: PrimeStar Max mastermix (Clontech) was used for high-fidelity PCR amplifications. .. Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech).

Article Title: The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae
Article Snippet: The absence of the target gene was confirmed by colony PCR. .. InFusion HD kits (Clontech) were used for cloning.

Transformation Assay:

Article Title: The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae
Article Snippet: Since the M2 strain is diploid, the transformed cells were sporulated, and the resulting tetrads dissected. .. InFusion HD kits (Clontech) were used for cloning.

Plasmid Preparation:

Article Title: A robotic multidimensional directed evolution approach applied to fluorescent voltage reporters
Article Snippet: The Rp BphP1/PAS-GAF domains and QuasAr2-ER2 genes were synthesized de novo (Genscript) with mammalian codon optimization and subcloned into the pN1 vector (Clontech) using Age I/ Not I sites. .. Ligations were performed using T4 DNA ligase (Fermentas) or InFusion HD kits (Clontech).

Article Title: The Fermentation Stress Response Protein Aaf1p/Yml081Wp Regulates Acetate Production in Saccharomyces cerevisiae
Article Snippet: Briefly, 788 bp upstream of the PGK1 gene were amplified from the pHVX2 vector , and cloned into the Sal1 site of pUG6. .. InFusion HD kits (Clontech) were used for cloning.

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    TaKaRa infusion hd kits
    Infusion Hd Kits, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 3 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/infusion hd kits/product/TaKaRa
    Average 94 stars, based on 3 article reviews
    Price from $9.99 to $1999.99
    infusion hd kits - by Bioz Stars, 2020-04
    94/100 stars
      Buy from Supplier

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