rabbit monoclonal antibodies against influenza virus ha  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Name:
    Influenza A Virus Hemagglutinin HA Antibody Rabbit MAb
    Description:
    This antibody was obtained from a rabbit immunized with purified recombinant Influenza A virus H1N1 Hemagglutinin extracellular domain
    Catalog Number:
    86001-RM01
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    Influenza
    Applications:
    WB,ELISA
    Immunogen:
    Recombinant H1N1 HA protein
    Antibody Type:
    MAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Sino Biological rabbit monoclonal antibodies against influenza virus ha
    This antibody was obtained from a rabbit immunized with purified recombinant Influenza A virus H1N1 Hemagglutinin extracellular domain
    https://www.bioz.com/result/rabbit monoclonal antibodies against influenza virus ha/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit monoclonal antibodies against influenza virus ha - by Bioz Stars, 2021-07
    94/100 stars

    Images

    Related Articles

    Staining:

    Article Title: A universal dual mechanism immunotherapy for the treatment of influenza virus infections
    Article Snippet: Virus-infected cells were then incubated at 37 °C for 30 min with 0.3 mL of 5 µg/mL goat anti-human F(ab′) -PE (Abcam, cat. no. ab98596). .. To identify virus-infected cells, the cells were concurrently stained with 0.3 mL of 10 µg/mL rabbit anti-hemagglutinin antibody (Sino Biological Inc, cat. no. 86001-RM01) followed by 0.3 mL of 10 µg/mL goat anti-rabbit IgG-AF647 (Invitrogen, cat. no. A-21244). .. After the final wash (2 × 0.3 mL), the transwell inserts were placed on glass slides and confocal images were acquired by confocal microscopy (FV 1000, Olympus).

    Nucleic Acid Electrophoresis:

    Article Title: mRNA Vaccines Encoding the HA Protein of Influenza A H1N1 Virus Delivered by Cationic Lipid Nanoparticles Induce Protective Immune Responses in Mice
    Article Snippet: The total protein of the cells was extracted, and a bicinchoninic acid assay kit (Biyuntian Biotechnology Co. Ltd., Shanghai, China) was used to measure the protein concentration. .. The same amount of protein was taken for SDS-polyacrylamide gel electrophoresis separation, transferred on to the nitrocellulose membrane (GE Healthcare, Little Chalfont, Buckinghamshire, UK), blocked with 5% skimmed milk, mixed with influenza A virus HA rabbit Mab (1:2000) (Sino Biological, Beijing, China) at room temperature for 2 h, and washed thrice with TBST for 8 min each. .. It was then incubated with the secondary antibody for 1 h at room temperature and washed thrice with TBST for 8 min each.

    Incubation:

    Article Title: mRNA Vaccines Encoding the HA Protein of Influenza A H1N1 Virus Delivered by Cationic Lipid Nanoparticles Induce Protective Immune Responses in Mice
    Article Snippet: The cells were fixed with 4% paraformaldehyde for 10 min and blocked with 2% BSA (bovine serum albumin) and 0.1% Triton X-100 in PBS for 30 min at room temperature 48 h after transfection. .. After washing with PBS twice, the cells were incubated with an influenza A virus HA antibody (Rabbit Mab; Sino Biological, Beijing, China) (1:500) for 1 h at 37 °C. .. FITC-conjugated goat anti-rabbit immunoglobulin G (IgG) antibodies (1:500) diluted in PBS were added and incubated for 1 h at room temperature.

    Labeling:

    Article Title: A universal dual mechanism immunotherapy for the treatment of influenza virus infections
    Article Snippet: The resulting cells were resuspended in freezing medium (90% FBS plus 10% DMSO) and stored at –80 °C for future flow cytometry analyses (if the cells are used immediately after digestion, they should be incubated with FBS to block the non-specific bindings of secondary antibodies). .. After thawing, cells were blocked with TruStain FcX™ antibody (Biolegend) and then labeled with rabbit anti-hemagglutinin antibody (1:100 dilution, Sino Biological Inc, cat. no. 86001-RM01) for 1 h on ice. .. Cells were washed 3x with PBS and then stained with goat anti-rat IgG-PE (1:500 dilution, Invitrogen, cat. no. A10545) and goat anti-rabbit IgG-AF647 (1:1000 dilution, Invitrogen, cat. no. A-21244) for 20 min on ice.

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Sino Biological influenza a virus hemagglutinin ha antibody rabbit mab
    Effect of λ-CGN on <t>influenza</t> A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice
    Influenza A Virus Hemagglutinin Ha Antibody Rabbit Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a virus hemagglutinin ha antibody rabbit mab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a virus hemagglutinin ha antibody rabbit mab - by Bioz Stars, 2021-07
    94/100 stars
      Buy from Supplier

    88
    Sino Biological h9n2 ha antibody
    The location of the AIV <t>H9N2</t> antigen and goose CD4 and CD8α molecules in several tissues at 5 dpi. The protein locations in the different tissues from AIV H9N2-infected birds were detected by IHC assay (Negative control group: a–g ). Viral positive signals and cells positive for CD4 or CD8α antigen appeared dark brown using immunohistochemical staining. SP: SP (A,H,O) , BF: BF (B,I,P) , SI: SI (C,J,Q) , R: R (D,K,R) , LU: lung (E,L,S) , TR: TR (F,M,T) , LI: LI (G,N,U) .
    H9n2 Ha Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 88/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/h9n2 ha antibody/product/Sino Biological
    Average 88 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    h9n2 ha antibody - by Bioz Stars, 2021-07
    88/100 stars
      Buy from Supplier

    Image Search Results


    Effect of λ-CGN on influenza A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice

    Journal: bioRxiv

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses in mice and severe acute respiratory syndrome coronavirus 2 in vitro

    doi: 10.1101/2020.08.23.255364

    Figure Lengend Snippet: Effect of λ-CGN on influenza A infection in vivo . BALB/c mice were mock-infected (black) or intranasally infected with maPR8 at 5 MLD50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, bright green) or a higher dose (5 mg/kg/d, dark green), followed by intranasal administration. Control mice received oral OSV-P twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight (A) and mortality (B) of mice were measured every day from Days 0 to 14 post-infection. Data are expressed as the mean ± SEM from five mice

    Article Snippet: Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Infection, In Vivo, Mouse Assay

    Effect of λ-CGN on the intracellular entry of influenza A virus. MDCK cells were infected with PR8 (MOI, 5) and subsequently mock-treated or treated either with λ-CGN or with p-KG03 at a concentration of 10 μg/ml. At 4 h p.i. in the absence of CHX (A) or at 2.5 h in the presence of 10 μg/ml CHX (B), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×.

    Journal: bioRxiv

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses in mice and severe acute respiratory syndrome coronavirus 2 in vitro

    doi: 10.1101/2020.08.23.255364

    Figure Lengend Snippet: Effect of λ-CGN on the intracellular entry of influenza A virus. MDCK cells were infected with PR8 (MOI, 5) and subsequently mock-treated or treated either with λ-CGN or with p-KG03 at a concentration of 10 μg/ml. At 4 h p.i. in the absence of CHX (A) or at 2.5 h in the presence of 10 μg/ml CHX (B), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×.

    Article Snippet: Viral NP and HA proteins were detected using mouse anti-NP (catalog no. 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (catalog no. 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Infection, Concentration Assay

    Construction and verification of mRNA vaccine encoding the H1N1-HA protein. ( a ) Agarose gel electrophoresis of  Xho I enzyme digestion products. 1 represents the intact plasmids of pGEM-H1N1-HA-n3 and 2 represents its linearized product of 5355 bp. M1: DL 5,000 DNA Marker (TaKaRa, Tokyo, Japan); M2: DL 2000 DNA Marker (TaKaRa, Tokyo, Japan). ( b,c ) Western blot and indirect immunofluorescence analyses. A549 cells were harvested 12 h and 48 h after transfection. The H1N1-HA protein was detected using rabbit anti-influenza A virus HA Mab. The H1N1-virus group was used as the positive control. Mock represents the negative control. DAPI was used to dye the nuclei.

    Journal: Vaccines

    Article Title: mRNA Vaccines Encoding the HA Protein of Influenza A H1N1 Virus Delivered by Cationic Lipid Nanoparticles Induce Protective Immune Responses in Mice

    doi: 10.3390/vaccines8010123

    Figure Lengend Snippet: Construction and verification of mRNA vaccine encoding the H1N1-HA protein. ( a ) Agarose gel electrophoresis of Xho I enzyme digestion products. 1 represents the intact plasmids of pGEM-H1N1-HA-n3 and 2 represents its linearized product of 5355 bp. M1: DL 5,000 DNA Marker (TaKaRa, Tokyo, Japan); M2: DL 2000 DNA Marker (TaKaRa, Tokyo, Japan). ( b,c ) Western blot and indirect immunofluorescence analyses. A549 cells were harvested 12 h and 48 h after transfection. The H1N1-HA protein was detected using rabbit anti-influenza A virus HA Mab. The H1N1-virus group was used as the positive control. Mock represents the negative control. DAPI was used to dye the nuclei.

    Article Snippet: The same amount of protein was taken for SDS-polyacrylamide gel electrophoresis separation, transferred on to the nitrocellulose membrane (GE Healthcare, Little Chalfont, Buckinghamshire, UK), blocked with 5% skimmed milk, mixed with influenza A virus HA rabbit Mab (1:2000) (Sino Biological, Beijing, China) at room temperature for 2 h, and washed thrice with TBST for 8 min each.

    Techniques: Agarose Gel Electrophoresis, Marker, Western Blot, Immunofluorescence, Transfection, Positive Control, Negative Control

    Antiviral effect of λ-CGN against influenza A virus in vivo. BALB/c mice (6–7 weeks old female) were mock-infected (black) or intranasally infected with maPR8 at 5 MLD 50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, purple) or a higher dose (5 mg/kg/d, green), followed by intranasal administration. Control mice received OSV-P orally twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight ( A ) and mortality ( B ) of mice were measured every day from days 0 to 14 post-infection. Data are expressed as the mean ± S.D. from five mice. Survival statistics were calculated by Log-rank (Mantel–Cox) test. ** P

    Journal: Scientific Reports

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2

    doi: 10.1038/s41598-020-80896-9

    Figure Lengend Snippet: Antiviral effect of λ-CGN against influenza A virus in vivo. BALB/c mice (6–7 weeks old female) were mock-infected (black) or intranasally infected with maPR8 at 5 MLD 50 (red). As test groups, the virus was preincubated at room temperature for 30 min with λ-CGN at a lower dose (1 mg/kg/d, purple) or a higher dose (5 mg/kg/d, green), followed by intranasal administration. Control mice received OSV-P orally twice a day (10 mg/kg/d) at 8-h intervals, starting at 4 h before viral infection (blue). Body weight ( A ) and mortality ( B ) of mice were measured every day from days 0 to 14 post-infection. Data are expressed as the mean ± S.D. from five mice. Survival statistics were calculated by Log-rank (Mantel–Cox) test. ** P

    Article Snippet: Influenza viral NP and HA proteins were detected using mouse anti-NP (Cat. No., 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (Cat. No., 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: In Vivo, Mouse Assay, Infection

    Effect of λ-CGN on the influenza A virus entry. ( A ) HA inhibition assay. Two-fold serially diluted PR8 (from 2 3 to 2 11 ) in PBS was incubated with an equal volume of PBS or twofold increasing concentrations of λ-CGN for 20 min. HA titer in each combination was determined at 30 min after addition of 0.5% chicken RBC. HA titers are marked on the right side of the panel. ( B , C ) Confocal microscopy. MDCK cells were infected with PR8 (MOI, 5) in the absence (Mock) or presence of either λ-CGN or p-KG03 at a concentration of 10 μg/ml. At 4 h post-infection in the absence of CHX ( B ) or at 2.5 h in the presence of 10 μg/ml CHX ( C ), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×. The images were analyzed using ZEN blue software 3.1 ( www.zeiss.com ).

    Journal: Scientific Reports

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2

    doi: 10.1038/s41598-020-80896-9

    Figure Lengend Snippet: Effect of λ-CGN on the influenza A virus entry. ( A ) HA inhibition assay. Two-fold serially diluted PR8 (from 2 3 to 2 11 ) in PBS was incubated with an equal volume of PBS or twofold increasing concentrations of λ-CGN for 20 min. HA titer in each combination was determined at 30 min after addition of 0.5% chicken RBC. HA titers are marked on the right side of the panel. ( B , C ) Confocal microscopy. MDCK cells were infected with PR8 (MOI, 5) in the absence (Mock) or presence of either λ-CGN or p-KG03 at a concentration of 10 μg/ml. At 4 h post-infection in the absence of CHX ( B ) or at 2.5 h in the presence of 10 μg/ml CHX ( C ), viral NP was detected with an anti-NP antibody and an Alex Fluor 488-conjugated goat anti-mouse secondary antibody (green). Cell nuclei were counterstained with DAPI (blue). Original magnification, 400×. The images were analyzed using ZEN blue software 3.1 ( www.zeiss.com ).

    Article Snippet: Influenza viral NP and HA proteins were detected using mouse anti-NP (Cat. No., 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (Cat. No., 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Inhibition, Incubation, Confocal Microscopy, Infection, Concentration Assay, Software

    Inhibition of infection by influenza A virus (H1N1) HA/NA- or SARS-CoV-2 spike-pseudotyped viruses. Lentiviral pseudotypes bearing influenza A virus (H1N1) HA and NA proteins (black bars) or SARS-CoV-2 spike protein (gray bars) were prepared, in which a firefly luciferase-expressing plasmid was incorporated. They were preincubated with mock or increasing concentrations of λ-CGN for 2 h at 37 °C and then infected into Vero E6 cells. On day 2, relative firefly luciferase activity (RLU) was determined by fixing the mock-treated sample at 100%. Data are expressed as the mean ± S.D. of three independent experiments. **** P

    Journal: Scientific Reports

    Article Title: Antiviral activity of lambda-carrageenan against influenza viruses and severe acute respiratory syndrome coronavirus 2

    doi: 10.1038/s41598-020-80896-9

    Figure Lengend Snippet: Inhibition of infection by influenza A virus (H1N1) HA/NA- or SARS-CoV-2 spike-pseudotyped viruses. Lentiviral pseudotypes bearing influenza A virus (H1N1) HA and NA proteins (black bars) or SARS-CoV-2 spike protein (gray bars) were prepared, in which a firefly luciferase-expressing plasmid was incorporated. They were preincubated with mock or increasing concentrations of λ-CGN for 2 h at 37 °C and then infected into Vero E6 cells. On day 2, relative firefly luciferase activity (RLU) was determined by fixing the mock-treated sample at 100%. Data are expressed as the mean ± S.D. of three independent experiments. **** P

    Article Snippet: Influenza viral NP and HA proteins were detected using mouse anti-NP (Cat. No., 11675-MM03; Sino Biological, Beijing, China) and rabbit anti-HA2 (Cat. No., 86001-RM01; Sino Biological) antibodies, respectively, according to our previous report .

    Techniques: Inhibition, Infection, Luciferase, Expressing, Plasmid Preparation, Activity Assay

    The location of the AIV H9N2 antigen and goose CD4 and CD8α molecules in several tissues at 5 dpi. The protein locations in the different tissues from AIV H9N2-infected birds were detected by IHC assay (Negative control group: a–g ). Viral positive signals and cells positive for CD4 or CD8α antigen appeared dark brown using immunohistochemical staining. SP: SP (A,H,O) , BF: BF (B,I,P) , SI: SI (C,J,Q) , R: R (D,K,R) , LU: lung (E,L,S) , TR: TR (F,M,T) , LI: LI (G,N,U) .

    Journal: Frontiers in Microbiology

    Article Title: LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies

    doi: 10.3389/fmicb.2016.00166

    Figure Lengend Snippet: The location of the AIV H9N2 antigen and goose CD4 and CD8α molecules in several tissues at 5 dpi. The protein locations in the different tissues from AIV H9N2-infected birds were detected by IHC assay (Negative control group: a–g ). Viral positive signals and cells positive for CD4 or CD8α antigen appeared dark brown using immunohistochemical staining. SP: SP (A,H,O) , BF: BF (B,I,P) , SI: SI (C,J,Q) , R: R (D,K,R) , LU: lung (E,L,S) , TR: TR (F,M,T) , LI: LI (G,N,U) .

    Article Snippet: Rabbit polyclonal anti-influenza A H9N2 HA antibody was purchased from Sino Biological Inc. (Shanghai, China) and diluted to 1:200.

    Techniques: Infection, Immunohistochemistry, Negative Control, Staining

    The relative transcription levels of immune-related genes in AIV H9N2-treated goose PBMCs at 6 h post-infection. IFNα, IFNγ, IL1β, IL6, and MyD88 mRNA expression was normalized using an internal control. The data were expressed as the mean ± SEM (n = 4), and the difference between the agonist-treated cells and the mock-treated cells was analyzed with a t -test. Groups denoted by one star ( ∗ ) represent a significant difference at P

    Journal: Frontiers in Microbiology

    Article Title: LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies

    doi: 10.3389/fmicb.2016.00166

    Figure Lengend Snippet: The relative transcription levels of immune-related genes in AIV H9N2-treated goose PBMCs at 6 h post-infection. IFNα, IFNγ, IL1β, IL6, and MyD88 mRNA expression was normalized using an internal control. The data were expressed as the mean ± SEM (n = 4), and the difference between the agonist-treated cells and the mock-treated cells was analyzed with a t -test. Groups denoted by one star ( ∗ ) represent a significant difference at P

    Article Snippet: Rabbit polyclonal anti-influenza A H9N2 HA antibody was purchased from Sino Biological Inc. (Shanghai, China) and diluted to 1:200.

    Techniques: Infection, Expressing

    Viral copies in the selected tissues from AIV H9N2-infected geese at 5 dpi. The efficiency of AIV M gene amplification by qRT-PCR was 100.1%, and R 2 was 0.998. The average viral copy number showed an evident difference between individual samples. The average number of viral copies was detected in the peripheral blood (10 9.68 copies/μg), feces (10 7.36 copies/μg), HG (10 6.74 copies/μg), TR (10 6.24 copies/μg), CT (10 5.64 copies/μg), T (10 5.63 copies/μg), BF (10 5.59 copies/μg), and R (10 5.37 copies/μg). The data are expressed as the mean ± SEM ( n = 3).

    Journal: Frontiers in Microbiology

    Article Title: LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies

    doi: 10.3389/fmicb.2016.00166

    Figure Lengend Snippet: Viral copies in the selected tissues from AIV H9N2-infected geese at 5 dpi. The efficiency of AIV M gene amplification by qRT-PCR was 100.1%, and R 2 was 0.998. The average viral copy number showed an evident difference between individual samples. The average number of viral copies was detected in the peripheral blood (10 9.68 copies/μg), feces (10 7.36 copies/μg), HG (10 6.74 copies/μg), TR (10 6.24 copies/μg), CT (10 5.64 copies/μg), T (10 5.63 copies/μg), BF (10 5.59 copies/μg), and R (10 5.37 copies/μg). The data are expressed as the mean ± SEM ( n = 3).

    Article Snippet: Rabbit polyclonal anti-influenza A H9N2 HA antibody was purchased from Sino Biological Inc. (Shanghai, China) and diluted to 1:200.

    Techniques: Infection, Amplification, Quantitative RT-PCR

    The histological changes in the LU (E) , LI (F) , SP (G) , and SI (H) in the AIV H9N2 strain-infected goslings at 5 dpi. Control groups were shown in (A–D) . The histological sections were stained with HE. Hyperaemia and congestion in the LU as well as cellular swelling, congestion, hepatic sinusoid disappearance, and suffuse hemorrhage in the SP were observed. Moderate pathological alterations in the SI were also observed.

    Journal: Frontiers in Microbiology

    Article Title: LPAIV H9N2 Drives the Differential Expression of Goose Interferons and Proinflammatory Cytokines in Both In Vitro and In Vivo Studies

    doi: 10.3389/fmicb.2016.00166

    Figure Lengend Snippet: The histological changes in the LU (E) , LI (F) , SP (G) , and SI (H) in the AIV H9N2 strain-infected goslings at 5 dpi. Control groups were shown in (A–D) . The histological sections were stained with HE. Hyperaemia and congestion in the LU as well as cellular swelling, congestion, hepatic sinusoid disappearance, and suffuse hemorrhage in the SP were observed. Moderate pathological alterations in the SI were also observed.

    Article Snippet: Rabbit polyclonal anti-influenza A H9N2 HA antibody was purchased from Sino Biological Inc. (Shanghai, China) and diluted to 1:200.

    Techniques: Infection, Staining