influenza a h5n1 hemagglutinin ha antibody rabbit pab  (Sino Biological)


Bioz Verified Symbol Sino Biological is a verified supplier
Bioz Manufacturer Symbol Sino Biological manufactures this product  
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 93
    Name:
    Influenza A H5N1 A Vietnam 1194 2004 Hemagglutinin HA Antibody Rabbit PAb Antigen Affinity Purified
    Description:
    Produced in rabbits immunized with purified recombinant Influenza A H5N1 A Vietnam 1194 2004 Hemagglutinin HA Catalog 11062 V08H1 AAT73273 1 Met1 Gln531 Influenza A H5N1 A Vietnam 1194 2004 Hemagglutinin HA specific IgG was purified by Influenza A H5N1 A Vietnam 1194 2004 Hemagglutinin HA affinity chromatography
    Catalog Number:
    11062-T54
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    H5N1
    Applications:
    WB,ELISA
    Immunogen:
    Recombinant Influenza A H5N1 (A/Vietnam/1194/2004) Hemagglutinin / HA Protein (Catalog#11062-V08H1)
    Antibody Type:
    PAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
    Buy from Supplier


    Structured Review

    Sino Biological influenza a h5n1 hemagglutinin ha antibody rabbit pab
    MAb-mediated protection against a lethal challenge of <t>H5N1</t> virus in mice
    Produced in rabbits immunized with purified recombinant Influenza A H5N1 A Vietnam 1194 2004 Hemagglutinin HA Catalog 11062 V08H1 AAT73273 1 Met1 Gln531 Influenza A H5N1 A Vietnam 1194 2004 Hemagglutinin HA specific IgG was purified by Influenza A H5N1 A Vietnam 1194 2004 Hemagglutinin HA affinity chromatography
    https://www.bioz.com/result/influenza a h5n1 hemagglutinin ha antibody rabbit pab/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 hemagglutinin ha antibody rabbit pab - by Bioz Stars, 2021-09
    93/100 stars

    Images

    1) Product Images from "Selection of Therapeutic H5N1 Monoclonal Antibodies Following IgVH Repertoire Analysis in Mice"

    Article Title: Selection of Therapeutic H5N1 Monoclonal Antibodies Following IgVH Repertoire Analysis in Mice

    Journal: Antiviral research

    doi: 10.1016/j.antiviral.2016.04.001

    MAb-mediated protection against a lethal challenge of H5N1 virus in mice
    Figure Legend Snippet: MAb-mediated protection against a lethal challenge of H5N1 virus in mice

    Techniques Used: Mouse Assay

    Related Articles

    HI Assay:

    Article Title: Selection of Therapeutic H5N1 Monoclonal Antibodies Following IgVH Repertoire Analysis in Mice
    Article Snippet: .. Hemagglutination inhibition (HI) activity specific to A/Vietnam/1194/2004 (H5N1), A/Cambodia/RO405050/2007 (H5N1), A/turkey/Turkey/1/2005 (H5N1), and A/H5N1/Anhui/1/05 (H5N1) virus (NIBSC, Hertfordshire England) were determined ( ). ..

    Activity Assay:

    Article Title: Selection of Therapeutic H5N1 Monoclonal Antibodies Following IgVH Repertoire Analysis in Mice
    Article Snippet: .. Hemagglutination inhibition (HI) activity specific to A/Vietnam/1194/2004 (H5N1), A/Cambodia/RO405050/2007 (H5N1), A/turkey/Turkey/1/2005 (H5N1), and A/H5N1/Anhui/1/05 (H5N1) virus (NIBSC, Hertfordshire England) were determined ( ). ..

    Similar Products

  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 94
    Sino Biological anti h7n9 hemagglutinin ha antibody rabbit pab
    Biological and chemical modifications of <t>H7N9</t> VLPs. (A) SDS-PAGE and western blot analysis of biologically modified H7N9 VLPs. Lane 1–3 represent 320 ng, 180 ng and 90 ng of αGal (-) VLP based on HA content, respectively. Lane 4–6 are 130 ng, 90, and 40 ng of αGal (+) VLP, respectively. (B) SDS-PAGE and western blot analysis of chemically modified H7N9 VLPs. S: protein standards; 1: αGal (-) H7N9 VLP; 2: intermediate of αGal (-) H7N9 VLP reacted with GO; 3: H7N9 conjugated with αGal linker a11; 4: H7N9 conjugated with αGal linker aN11; 5: H7N9 VLPs conjugated with control linker sp11. (C) Electron microscopy of H7N9 VLPs. (D) Electron microscopy of H7N9 VLPs stained with gold particles. Negative: VLPs were only stained with immunogold donkey anti-rabbit secondary antibody; Positive: VLPs were stained with anti-HA7 rabbit polyclonal antibody, followed by staining with immunogold donkey anti-rabbit secondary antibody. Sp11 is the aminooxy spacer control linker without αGal antigen; a11 is one type of αGal (Galα1,3-Galβ1,4-Glc) aminooxy linker; aN11 is the other type of αGal (Galα1,3-Galβ1,4-GlcNAc) aminooxy linker.
    Anti H7n9 Hemagglutinin Ha Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/anti h7n9 hemagglutinin ha antibody rabbit pab/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    anti h7n9 hemagglutinin ha antibody rabbit pab - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    93
    Sino Biological influenza a h5n1 hemagglutinin ha antibody rabbit pab
    MAb-mediated protection against a lethal challenge of <t>H5N1</t> virus in mice
    Influenza A H5n1 Hemagglutinin Ha Antibody Rabbit Pab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 hemagglutinin ha antibody rabbit pab/product/Sino Biological
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 hemagglutinin ha antibody rabbit pab - by Bioz Stars, 2021-09
    93/100 stars
      Buy from Supplier

    94
    Sino Biological polyclonal rabbit anti h5n1 antibody
    Hemagglutination assay of <t>H5N1</t> viruses with or without A134V substitution. Comparative hemagglutination ability of four wild-type Cambodian H5N1 isolates: A/Cambodia/408008/2005 (A134V; HA accession number: HQ664938), A/Cambodia/V0401301/2011 (A134V; HA accession number: JN588807), A/Cambodia/V0203306/2011 (A134; HA accession number: JN588805) and A/Cambodia/V0219301/2011(A134; HA accession number: JN588806). Suspensions (0.75%) of human type O, horse and guinea pig blood were used for hemagglutination assays. The two isolates with valine at position 134 were unable to agglutinate horse red blood cells. Results represent the average of two independent experiments.
    Polyclonal Rabbit Anti H5n1 Antibody, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/polyclonal rabbit anti h5n1 antibody/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    polyclonal rabbit anti h5n1 antibody - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    Image Search Results


    Biological and chemical modifications of H7N9 VLPs. (A) SDS-PAGE and western blot analysis of biologically modified H7N9 VLPs. Lane 1–3 represent 320 ng, 180 ng and 90 ng of αGal (-) VLP based on HA content, respectively. Lane 4–6 are 130 ng, 90, and 40 ng of αGal (+) VLP, respectively. (B) SDS-PAGE and western blot analysis of chemically modified H7N9 VLPs. S: protein standards; 1: αGal (-) H7N9 VLP; 2: intermediate of αGal (-) H7N9 VLP reacted with GO; 3: H7N9 conjugated with αGal linker a11; 4: H7N9 conjugated with αGal linker aN11; 5: H7N9 VLPs conjugated with control linker sp11. (C) Electron microscopy of H7N9 VLPs. (D) Electron microscopy of H7N9 VLPs stained with gold particles. Negative: VLPs were only stained with immunogold donkey anti-rabbit secondary antibody; Positive: VLPs were stained with anti-HA7 rabbit polyclonal antibody, followed by staining with immunogold donkey anti-rabbit secondary antibody. Sp11 is the aminooxy spacer control linker without αGal antigen; a11 is one type of αGal (Galα1,3-Galβ1,4-Glc) aminooxy linker; aN11 is the other type of αGal (Galα1,3-Galβ1,4-GlcNAc) aminooxy linker.

    Journal: PLoS ONE

    Article Title: Addition of αGal HyperAcute™ technology to recombinant avian influenza vaccines induces strong low-dose antibody responses

    doi: 10.1371/journal.pone.0182683

    Figure Lengend Snippet: Biological and chemical modifications of H7N9 VLPs. (A) SDS-PAGE and western blot analysis of biologically modified H7N9 VLPs. Lane 1–3 represent 320 ng, 180 ng and 90 ng of αGal (-) VLP based on HA content, respectively. Lane 4–6 are 130 ng, 90, and 40 ng of αGal (+) VLP, respectively. (B) SDS-PAGE and western blot analysis of chemically modified H7N9 VLPs. S: protein standards; 1: αGal (-) H7N9 VLP; 2: intermediate of αGal (-) H7N9 VLP reacted with GO; 3: H7N9 conjugated with αGal linker a11; 4: H7N9 conjugated with αGal linker aN11; 5: H7N9 VLPs conjugated with control linker sp11. (C) Electron microscopy of H7N9 VLPs. (D) Electron microscopy of H7N9 VLPs stained with gold particles. Negative: VLPs were only stained with immunogold donkey anti-rabbit secondary antibody; Positive: VLPs were stained with anti-HA7 rabbit polyclonal antibody, followed by staining with immunogold donkey anti-rabbit secondary antibody. Sp11 is the aminooxy spacer control linker without αGal antigen; a11 is one type of αGal (Galα1,3-Galβ1,4-Glc) aminooxy linker; aN11 is the other type of αGal (Galα1,3-Galβ1,4-GlcNAc) aminooxy linker.

    Article Snippet: Following a 30-minute incubation in a glass petri dish incubation chamber, the grid was stained in a 40 μL drop of rabbit polyclonal anti-HA7 antibody (Sino Biological Inc., 40103-RP02, 1: 500 dilution in blocking buffer) or blocking buffer alone to serve as a negative control.

    Techniques: SDS Page, Western Blot, Modification, Electron Microscopy, Staining

    Expression and characterization of H7N9 VLP. a The viral titers of three recombinant baculoviruses were determined by plaque assay. The titer of recombinant baculovirus-HA (rBac-HA) was approxmiately 6 × 10 6 PFU/ml ( left ), the rBac-NA 3 × 10 7 PFU/ml (center), and the rBac-M1 1.1 × 10 8 PFU/ml ( right ). The protein expression of HA, NA, and M1 was identified respectively by immunofluorescence assay (lower panel). b Sf9 cells were co-infected with rBac-HA, rBac-NA, and rBac-M1 under various MOI combinations, and the VLPs were purified via sucrose gradient centrifugation. Formulation derived from the combinations of rBac-HA (MOI 2), rBac-NA (MOI 2), and rBac-M1 (MOI 2) co-infection exhibited the highest HA activity. c H7N9 VLP, H7/HA protein, N9/NA protein, and M1 proteins were detected by Western blot

    Journal: BMC Biotechnology

    Article Title: Multi-antigen avian influenza a (H7N9) virus-like particles: particulate characterizations and immunogenicity evaluation in murine and avian models

    doi: 10.1186/s12896-016-0321-6

    Figure Lengend Snippet: Expression and characterization of H7N9 VLP. a The viral titers of three recombinant baculoviruses were determined by plaque assay. The titer of recombinant baculovirus-HA (rBac-HA) was approxmiately 6 × 10 6 PFU/ml ( left ), the rBac-NA 3 × 10 7 PFU/ml (center), and the rBac-M1 1.1 × 10 8 PFU/ml ( right ). The protein expression of HA, NA, and M1 was identified respectively by immunofluorescence assay (lower panel). b Sf9 cells were co-infected with rBac-HA, rBac-NA, and rBac-M1 under various MOI combinations, and the VLPs were purified via sucrose gradient centrifugation. Formulation derived from the combinations of rBac-HA (MOI 2), rBac-NA (MOI 2), and rBac-M1 (MOI 2) co-infection exhibited the highest HA activity. c H7N9 VLP, H7/HA protein, N9/NA protein, and M1 proteins were detected by Western blot

    Article Snippet: Three days later, the monolayer of cells was washed and fixed with 80% acetone at −20 °C for 20 min. Rabbit polyclonal antibody against H7/HA peptide (Sino Biological, Beijing, China), rabbit polyclonal antibody against N9/NA peptide (ProSci, Poway, CA), and mouse polyclonal antibody against a recombinant M1 protein were applied at 1:10,000, 1:2,000, and 1:1,000 dilutions respectively and incubated for 1 h. After PBS wash, cells were further incubated with FITC-conjugated anti-rabbit or anti-mouse IgG (Jackson ImmunoResearch Laboratories, West Grove, PA).

    Techniques: Expressing, Recombinant, Plaque Assay, Immunofluorescence, Infection, Purification, Gradient Centrifugation, Derivative Assay, Activity Assay, Western Blot

    MAb-mediated protection against a lethal challenge of H5N1 virus in mice

    Journal: Antiviral research

    Article Title: Selection of Therapeutic H5N1 Monoclonal Antibodies Following IgVH Repertoire Analysis in Mice

    doi: 10.1016/j.antiviral.2016.04.001

    Figure Lengend Snippet: MAb-mediated protection against a lethal challenge of H5N1 virus in mice

    Article Snippet: Hemagglutination inhibition (HI) activity specific to A/Vietnam/1194/2004 (H5N1), A/Cambodia/RO405050/2007 (H5N1), A/turkey/Turkey/1/2005 (H5N1), and A/H5N1/Anhui/1/05 (H5N1) virus (NIBSC, Hertfordshire England) were determined ( ).

    Techniques: Mouse Assay

    Hemagglutination assay of H5N1 viruses with or without A134V substitution. Comparative hemagglutination ability of four wild-type Cambodian H5N1 isolates: A/Cambodia/408008/2005 (A134V; HA accession number: HQ664938), A/Cambodia/V0401301/2011 (A134V; HA accession number: JN588807), A/Cambodia/V0203306/2011 (A134; HA accession number: JN588805) and A/Cambodia/V0219301/2011(A134; HA accession number: JN588806). Suspensions (0.75%) of human type O, horse and guinea pig blood were used for hemagglutination assays. The two isolates with valine at position 134 were unable to agglutinate horse red blood cells. Results represent the average of two independent experiments.

    Journal: PLoS ONE

    Article Title: A Single Residue Substitution in the Receptor-Binding Domain of H5N1 Hemagglutinin Is Critical for Packaging into Pseudotyped Lentiviral Particles

    doi: 10.1371/journal.pone.0043596

    Figure Lengend Snippet: Hemagglutination assay of H5N1 viruses with or without A134V substitution. Comparative hemagglutination ability of four wild-type Cambodian H5N1 isolates: A/Cambodia/408008/2005 (A134V; HA accession number: HQ664938), A/Cambodia/V0401301/2011 (A134V; HA accession number: JN588807), A/Cambodia/V0203306/2011 (A134; HA accession number: JN588805) and A/Cambodia/V0219301/2011(A134; HA accession number: JN588806). Suspensions (0.75%) of human type O, horse and guinea pig blood were used for hemagglutination assays. The two isolates with valine at position 134 were unable to agglutinate horse red blood cells. Results represent the average of two independent experiments.

    Article Snippet: Surface labelling of H5-HA293T cells transfected with H5 HA were detached with and resuspended in PBS, blocked in 10% horse serum and then labelled with a polyclonal rabbit anti-H5N1 antibody (Sino Biologicals Inc., Beijing, China) at a 1∶400 dilution for 1 hr at 4°C.

    Techniques: Hemagglutination Assay