influenza a virus h5n1 avian flu ha  (Sino Biological)


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  • 91
    Name:
    Influenza A H5N1 Hemagglutinin HA Antibody Rabbit MAb
    Description:
    This antibody was obtained from a rabbit immunized with purified recombinant Influenza A virus H5N1 Avian Flu Hemagglutinin extracellular domain
    Catalog Number:
    11048-RM07
    Price:
    None
    Category:
    Primary Antibody
    Reactivity:
    H5N1
    Applications:
    WB,ELISA
    Immunogen:
    Recombinant H5N1 HA protein (Catalog#11048-V08H1)
    Antibody Type:
    MAb
    Host:
    Rabbit
    Isotype:
    Rabbit IgG
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    Structured Review

    Sino Biological influenza a virus h5n1 avian flu ha
    This antibody was obtained from a rabbit immunized with purified recombinant Influenza A virus H5N1 Avian Flu Hemagglutinin extracellular domain
    https://www.bioz.com/result/influenza a virus h5n1 avian flu ha/product/Sino Biological
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a virus h5n1 avian flu ha - by Bioz Stars, 2021-09
    91/100 stars

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    Related Articles

    Recombinant:

    Article Title: Neu5Acα2,6Gal and Neu5Acα2,3Gal receptor specificities on influenza viruses determined by a waveguide-mode sensor.
    Article Snippet: .. To characterize the differences in the receptor-binding specificities of human and avian influenza viruses with glycan chains, the authors performed binding analyses using an evanescent field-coupled waveguide-mode biosensor. ..

    Staining:

    Article Title: Glycosylation of the Hemagglutinin Protein of H5N1 Influenza Virus Increases Its Virulence in Mice by Exacerbating the Host Immune Response
    Article Snippet: .. The samples were stained with a rabbit monoclonal antibody to influenza A virus H5N1 HA protein (11048-RM07; Sino Biological Inc.) and with Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody (A-11034; Thermo Fisher Scientific). ..

    Binding Assay:

    Article Title: Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension.
    Article Snippet: .. Nanobodies consist of a single domain variable fragment of a camelid heavy-chain antibody. ..

    Purification:

    Article Title: Fenobody: A Ferritin-Displayed Nanobody with High Apparent Affinity and Half-Life Extension.
    Article Snippet: .. Nanobodies consist of a single domain variable fragment of a camelid heavy-chain antibody. ..

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  • 94
    Sino Biological ha rabbit igg
    Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A <t>H5N1</t> (A/Vietnam/1194/2004) HA <t>IgG,</t> followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).
    Ha Rabbit Igg, supplied by Sino Biological, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ha rabbit igg/product/Sino Biological
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    ha rabbit igg - by Bioz Stars, 2021-09
    94/100 stars
      Buy from Supplier

    92
    Sino Biological influenza a h5n1 hemagglutinin ha antibody rabbit mab
    Expression of viral proteins in infected MDCK cells. MDCK cells were infected with viruses at an MOI of 5. The infected cells were collected at 12 h postinfection, lysed in SDS loading buffer, and further analyzed by Western blotting (A and B). (A) Protein bands of HA, NP, and M1 proteins detected by Western blotting. The intensity of each band was measured by using ImageJ software, and relative intensity ratios of HA, NP, and M1 compared with that of GAPDH were calculated (B). The infected cells were digested with trypsin to obtain a single cell suspension at 12 h postinfection. The samples were stained with rabbit monoclonal antibody to <t>influenza</t> A virus <t>H5N1</t> HA protein and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and then detected on a FACSAria II (BD Biosciences). (C) Mean fluorescence intensity of HA protein was analyzed with FlowJo X 10.0.7r2 (Tree Star, San Carlos, CA). Data are presented as means ± SD ( n = 3). **, P
    Influenza A H5n1 Hemagglutinin Ha Antibody Rabbit Mab, supplied by Sino Biological, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/influenza a h5n1 hemagglutinin ha antibody rabbit mab/product/Sino Biological
    Average 92 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    influenza a h5n1 hemagglutinin ha antibody rabbit mab - by Bioz Stars, 2021-09
    92/100 stars
      Buy from Supplier

    86
    Sino Biological rabbit anti h7n7 for ha
    Expression of viral proteins in infected MDCK cells. MDCK cells were infected with viruses at an MOI of 5. The infected cells were collected at 12 h postinfection, lysed in SDS loading buffer, and further analyzed by Western blotting (A and B). (A) Protein bands of HA, NP, and M1 proteins detected by Western blotting. The intensity of each band was measured by using ImageJ software, and relative intensity ratios of HA, NP, and M1 compared with that of GAPDH were calculated (B). The infected cells were digested with trypsin to obtain a single cell suspension at 12 h postinfection. The samples were stained with rabbit monoclonal antibody to <t>influenza</t> A virus <t>H5N1</t> HA protein and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and then detected on a FACSAria II (BD Biosciences). (C) Mean fluorescence intensity of HA protein was analyzed with FlowJo X 10.0.7r2 (Tree Star, San Carlos, CA). Data are presented as means ± SD ( n = 3). **, P
    Rabbit Anti H7n7 For Ha, supplied by Sino Biological, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/rabbit anti h7n7 for ha/product/Sino Biological
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    rabbit anti h7n7 for ha - by Bioz Stars, 2021-09
    86/100 stars
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    Image Search Results


    Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A H5N1 (A/Vietnam/1194/2004) HA IgG, followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).

    Journal: Viruses

    Article Title: Novel Mutations Evading Avian Immunity around the Receptor Binding Site of the Clade 2.3.2.1c Hemagglutinin Gene Reduce Viral Thermostability and Mammalian Pathogenicity

    doi: 10.3390/v11100923

    Figure Lengend Snippet: Verification of 144N-glycosylation by Western blotting. Recombinant viruses untreated and treated with PNGase F enzyme were denatured and separated by SDS-PAGE. Transferred membranes were incubated with rabbit anti-influenza A H5N1 (A/Vietnam/1194/2004) HA IgG, followed by goat anti-rabbit IgG HRP-conjugated secondary antibody. Then, HRP was developed by TMB substrate. HA proteins of rH5N1 and rH5N1-I223V had 144N-glycan, and they had higher molecular weight than rH5N1-N144S and rH5N1-N144S-I223V in the absence of PNGase F enzyme treatment (−). However, the difference disappeared after treatment of PNGase F enzyme (+).

    Article Snippet: The proteins were transferred to a nitrocellulose membrane (Life Technologies Co.), and the membrane was incubated with anti-H5N1 virus (A/Vietnam/1194/2004), HA rabbit IgG (Sino Biological Inc., Beijing, China), followed by incubation with horseradish peroxidase conjugated-goat anti-rabbit IgG (Bethyl Laboratories Inc., Montgomery, AL, USA).

    Techniques: Western Blot, Recombinant, SDS Page, Incubation, Molecular Weight

    Expression of viral proteins in infected MDCK cells. MDCK cells were infected with viruses at an MOI of 5. The infected cells were collected at 12 h postinfection, lysed in SDS loading buffer, and further analyzed by Western blotting (A and B). (A) Protein bands of HA, NP, and M1 proteins detected by Western blotting. The intensity of each band was measured by using ImageJ software, and relative intensity ratios of HA, NP, and M1 compared with that of GAPDH were calculated (B). The infected cells were digested with trypsin to obtain a single cell suspension at 12 h postinfection. The samples were stained with rabbit monoclonal antibody to influenza A virus H5N1 HA protein and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and then detected on a FACSAria II (BD Biosciences). (C) Mean fluorescence intensity of HA protein was analyzed with FlowJo X 10.0.7r2 (Tree Star, San Carlos, CA). Data are presented as means ± SD ( n = 3). **, P

    Journal: Journal of Virology

    Article Title: Glycosylation of the Hemagglutinin Protein of H5N1 Influenza Virus Increases Its Virulence in Mice by Exacerbating the Host Immune Response

    doi: 10.1128/JVI.02215-16

    Figure Lengend Snippet: Expression of viral proteins in infected MDCK cells. MDCK cells were infected with viruses at an MOI of 5. The infected cells were collected at 12 h postinfection, lysed in SDS loading buffer, and further analyzed by Western blotting (A and B). (A) Protein bands of HA, NP, and M1 proteins detected by Western blotting. The intensity of each band was measured by using ImageJ software, and relative intensity ratios of HA, NP, and M1 compared with that of GAPDH were calculated (B). The infected cells were digested with trypsin to obtain a single cell suspension at 12 h postinfection. The samples were stained with rabbit monoclonal antibody to influenza A virus H5N1 HA protein and Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody and then detected on a FACSAria II (BD Biosciences). (C) Mean fluorescence intensity of HA protein was analyzed with FlowJo X 10.0.7r2 (Tree Star, San Carlos, CA). Data are presented as means ± SD ( n = 3). **, P

    Article Snippet: The samples were stained with a rabbit monoclonal antibody to influenza A virus H5N1 HA protein (11048-RM07; Sino Biological Inc.) and with Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody (A-11034; Thermo Fisher Scientific).

    Techniques: Expressing, Infection, Western Blot, Software, Staining, Fluorescence