incubator  (Thermo Fisher)


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    Structured Review

    Thermo Fisher incubator
    Incubator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 260 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incubator/product/Thermo Fisher
    Average 99 stars, based on 260 article reviews
    Price from $9.99 to $1999.99
    incubator - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Magnetic Beads:

    Article Title: NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus
    Article Snippet: For endogenous IPs, whole-cell lysates or nuclear extracts (10–20 mg) were mixed with Protein-G magnetic beads (Invitrogen; 30 µl of a 50% slurry) and anti-NPM1 (FC-61991), C-terminal anti-PIDD (Anto1 or AL233), or N-terminal anti-PIDD (S-17) antibody (3–5 µg, 1 ml final volume) overnight at 4°C on a rotating wheel. .. Beads were then washed three times with PBS, resolved by SDS-PAGE, and probed with anti-PIDD (H-300 [1:200 dilution]), anti-PIDD (Anto-1), anti-NPM1 (#3542), or anti-PIDD (S-17) detected with the corresponding secondary antibodies or mouse TrueBlot HRP-conjugated secondary antibodies [eBioscience]).

    Infection:

    Article Title: Effect of Bacteriophage Infection in Combination with Tobramycin on the Emergence of Resistance in Escherichia coli and Pseudomonas aeruginosa Biofilms
    Article Snippet: Bacteria and Bacteriophage Escherichia coli B (ATCC 11303) and Pseudomonas aeruginosa PAO1 (obtained from V. Deretic, University of New Mexico) were grown in Luria-Bertani (LB) broth (Accumedia Manufacturers, Inc., Lansing, Michigan, MI, USA) at 37 °C in an orbital rotating shaker water bath (Lab-Line Instruments, Inc. model 3540 Orbital Shaker Bath, Melrose Park, IL, USA). .. Bacteriophage T4 and PB-1 stocks were prepared by infecting early log phase of E. coli or P. aeruginosa , respectively, at a multiplicity of infection (MOI) of approximately 1000.

    Immunoprecipitation:

    Article Title: NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus
    Article Snippet: Lysates for immunoprecipitation were prepared in 1% or 0.1% NP-40 buffer (1% or 0.1% NP-40, 50 mM Tris-HCl, pH 8.0, 150–250 mM NaCl, 5 mM EDTA, 1 mM PMSF, protease inhibitors cocktail [Complete Mini; Roche], and phosphatase inhibitor cocktail [PhosSTOP; Roche]). .. Beads were then washed three times with PBS, resolved by SDS-PAGE, and probed with anti-PIDD (H-300 [1:200 dilution]), anti-PIDD (Anto-1), anti-NPM1 (#3542), or anti-PIDD (S-17) detected with the corresponding secondary antibodies or mouse TrueBlot HRP-conjugated secondary antibodies [eBioscience]).

    other:

    Article Title: Automated microbeam observation environment for biological analysis—Custom portable environmental control applied to a vertical microbeam system
    Article Snippet: The dish was then placed in a Series 8000 Direct-Heat CO2 Incubator (Thermo Fisher Scientific) at 37 °C and 5% CO2 for 30 min. Once removed from the incubator the dish was rinsed 3 times with 5 ml of sterile phosphate buffered saline solution.

    Western Blot:

    Article Title: NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus
    Article Snippet: Beads were then washed three times with PBS, resolved by SDS-PAGE, and probed with anti-PIDD (H-300 [1:200 dilution]), anti-PIDD (Anto-1), anti-NPM1 (#3542), or anti-PIDD (S-17) detected with the corresponding secondary antibodies or mouse TrueBlot HRP-conjugated secondary antibodies [eBioscience]). .. Beads were then washed three times with PBS, resolved by SDS-PAGE, and analyzed by Western blot.

    SDS Page:

    Article Title: NPM1 directs PIDDosome-dependent caspase-2 activation in the nucleolus
    Article Snippet: .. Beads were then washed three times with PBS, resolved by SDS-PAGE, and probed with anti-PIDD (H-300 [1:200 dilution]), anti-PIDD (Anto-1), anti-NPM1 (#3542), or anti-PIDD (S-17) detected with the corresponding secondary antibodies or mouse TrueBlot HRP-conjugated secondary antibodies [eBioscience]). ..

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    Thermo Fisher iems incubator shaker ht
    Effect of different orbital shaking conditions (900, 1150 or 1400 rpm) on RT-QuIC sensitivity using an <t>iEMS</t> multi-plate, temperature-controlled shaker incubator. Quadruplicate RT-QuIC reactions were seeded with 2 μL of scrapie positive (263K) brain tissue dilutions as indicated. Reactions were incubated at 42 °C in an orbital shaking Thermo Scientific (iEMS Incubator/Shaker HT) using Ha rPrP Sen (90–231) as the substrate. After a 17 h incubation, ThT fluorescence was measured in a <t>BMG</t> FLUOstar Omega plate reader using the RT-QuIC read settings described in Materials and Methods. The percentage of positive replicate wells is plotted for each dilution used.
    Iems Incubator Shaker Ht, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/iems incubator shaker ht/product/Thermo Fisher
    Average 99 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    iems incubator shaker ht - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    80
    Thermo Fisher high capacity incubator cytomat 24c
    Hestia in Motion 1 -Track view of west end of the platform, showing the INCA2000, <t>Cytomat</t> <t>24C</t> and the Washer/Combi section; 2- Hestia accessing a top positioned MultiDrop Combi; 3- Hestia accessing a ELx405 Washer; 4- Hestia accessing the INCA2000 stage nest.
    High Capacity Incubator Cytomat 24c, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 80/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/high capacity incubator cytomat 24c/product/Thermo Fisher
    Average 80 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    high capacity incubator cytomat 24c - by Bioz Stars, 2020-03
    80/100 stars
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    99
    Thermo Fisher heracell incubator
    Schematic of Transwell assay for measuring Tenofovir transport into and through an excised tissue specimen. Circular porcine buccal tissue specimens were maintained in Transwell supports, under which was a bath of PBS to hydrate the tissue. Matrigel was used to create a gelatinous seal around tissue edges to prevent leakage. An isotonic solution of 1% Tenofovir in PBS was applied to the apical surface. The assay was maintained at 37°C and 5% CO 2 in a <t>Heracell</t> incubator for 30 min to 6 h.
    Heracell Incubator, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/heracell incubator/product/Thermo Fisher
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    heracell incubator - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Effect of different orbital shaking conditions (900, 1150 or 1400 rpm) on RT-QuIC sensitivity using an iEMS multi-plate, temperature-controlled shaker incubator. Quadruplicate RT-QuIC reactions were seeded with 2 μL of scrapie positive (263K) brain tissue dilutions as indicated. Reactions were incubated at 42 °C in an orbital shaking Thermo Scientific (iEMS Incubator/Shaker HT) using Ha rPrP Sen (90–231) as the substrate. After a 17 h incubation, ThT fluorescence was measured in a BMG FLUOstar Omega plate reader using the RT-QuIC read settings described in Materials and Methods. The percentage of positive replicate wells is plotted for each dilution used.

    Journal: Viruses

    Article Title: Factors That Improve RT-QuIC Detection of Prion Seeding Activity

    doi: 10.3390/v8050140

    Figure Lengend Snippet: Effect of different orbital shaking conditions (900, 1150 or 1400 rpm) on RT-QuIC sensitivity using an iEMS multi-plate, temperature-controlled shaker incubator. Quadruplicate RT-QuIC reactions were seeded with 2 μL of scrapie positive (263K) brain tissue dilutions as indicated. Reactions were incubated at 42 °C in an orbital shaking Thermo Scientific (iEMS Incubator/Shaker HT) using Ha rPrP Sen (90–231) as the substrate. After a 17 h incubation, ThT fluorescence was measured in a BMG FLUOstar Omega plate reader using the RT-QuIC read settings described in Materials and Methods. The percentage of positive replicate wells is plotted for each dilution used.

    Article Snippet: Plates were sealed (Nalgene Nunc International sealer; 232702) and incubated in a BMG Fluostar plate reader (BMG Labtech, Cary, NC, USA) or a ThermoFisher iEMS Incubator/Shaker HT (Rockford, IL, USA) multiplate incubator/shaker as indicated, for 45–90 h with cycles of 60 s shaking and 60 s of rest throughout the incubation, unless otherwise indicated.

    Techniques: Incubation, Fluorescence

    Hestia in Motion 1 -Track view of west end of the platform, showing the INCA2000, Cytomat 24C and the Washer/Combi section; 2- Hestia accessing a top positioned MultiDrop Combi; 3- Hestia accessing a ELx405 Washer; 4- Hestia accessing the INCA2000 stage nest.

    Journal: Journal of laboratory automation

    Article Title: Designs and Concept-Reliance of a Fully Automated High Content Screening Platform

    doi: 10.1177/2211068212453311

    Figure Lengend Snippet: Hestia in Motion 1 -Track view of west end of the platform, showing the INCA2000, Cytomat 24C and the Washer/Combi section; 2- Hestia accessing a top positioned MultiDrop Combi; 3- Hestia accessing a ELx405 Washer; 4- Hestia accessing the INCA2000 stage nest.

    Article Snippet: A high capacity incubator Cytomat 24C (Thermo Fisher Scientific), set at 37°C, 5% CO2 and 98% humidity and a Cytomat Hotel (Thermo Fisher Scientific) ambient temperature incubator, are used for sample plate incubation and storage, varying on the onboard assay conditions.

    Techniques:

    Schematic of Transwell assay for measuring Tenofovir transport into and through an excised tissue specimen. Circular porcine buccal tissue specimens were maintained in Transwell supports, under which was a bath of PBS to hydrate the tissue. Matrigel was used to create a gelatinous seal around tissue edges to prevent leakage. An isotonic solution of 1% Tenofovir in PBS was applied to the apical surface. The assay was maintained at 37°C and 5% CO 2 in a Heracell incubator for 30 min to 6 h.

    Journal: PLoS ONE

    Article Title: Quantitative Analysis of Microbicide Concentrations in Fluids, Gels and Tissues Using Confocal Raman Spectroscopy

    doi: 10.1371/journal.pone.0085124

    Figure Lengend Snippet: Schematic of Transwell assay for measuring Tenofovir transport into and through an excised tissue specimen. Circular porcine buccal tissue specimens were maintained in Transwell supports, under which was a bath of PBS to hydrate the tissue. Matrigel was used to create a gelatinous seal around tissue edges to prevent leakage. An isotonic solution of 1% Tenofovir in PBS was applied to the apical surface. The assay was maintained at 37°C and 5% CO 2 in a Heracell incubator for 30 min to 6 h.

    Article Snippet: Freshly excised tissue specimens (7-mm diameter; 1-mm thickness; n=3) were incubated while fully submerged in 1% Tenofovir clinical gel and maintained at 5% CO2 and 37 °C for different amounts of time (2, 6, 9, and 24 h) in a Heracell incubator (Thermo Scientific, Asheville, NC).

    Techniques: Transwell Assay

    Raman spectra for clinical 1% Tenofovir (TFV) gel. Fresh porcine buccal tissue specimens (7-mm diameter, 1-mm thickness) were incubated by fully submerging them in 1% Tenofovir test gel (solid line) and HEC placebo gel (dotted line) for 6 h at 37°C and 5% CO 2 in a Heracell incubator. The recorded Raman spectra demonstrated that the Tenofovir band was clearly distinguishable from Raman peaks due to the gel and tissue. β, bending; ν, stretching ; τ, twisting; r, rocking.

    Journal: PLoS ONE

    Article Title: Quantitative Analysis of Microbicide Concentrations in Fluids, Gels and Tissues Using Confocal Raman Spectroscopy

    doi: 10.1371/journal.pone.0085124

    Figure Lengend Snippet: Raman spectra for clinical 1% Tenofovir (TFV) gel. Fresh porcine buccal tissue specimens (7-mm diameter, 1-mm thickness) were incubated by fully submerging them in 1% Tenofovir test gel (solid line) and HEC placebo gel (dotted line) for 6 h at 37°C and 5% CO 2 in a Heracell incubator. The recorded Raman spectra demonstrated that the Tenofovir band was clearly distinguishable from Raman peaks due to the gel and tissue. β, bending; ν, stretching ; τ, twisting; r, rocking.

    Article Snippet: Freshly excised tissue specimens (7-mm diameter; 1-mm thickness; n=3) were incubated while fully submerged in 1% Tenofovir clinical gel and maintained at 5% CO2 and 37 °C for different amounts of time (2, 6, 9, and 24 h) in a Heracell incubator (Thermo Scientific, Asheville, NC).

    Techniques: Incubation

    Tenofovir concentrations vs. time in tissue and the top fluid compartment in the Transwell assay . Transwell assay experiments were performed to characterize the transport of Tenofovir into and through tissue after microbicide application. The Transwell setup was maintained at 37°C and 5% CO 2 in a Heracell incubator for different amounts of time, ranging from 30 min to 6 h. After incubation, the tissue (7-mm diameter, 1-mm thickness) and fluids were isolated and stored at −80°C. Thawed tissue specimens and fluids were scanned. Quantification of Tenofovir concentrations was performed by interpolation, referencing to calibration curves of Tenofovir in tissue homogenates and fluids (shown in Figure 4 ). Each data point gives the median, and the smallest and largest measurements in the 3 independent experiments (n=3).

    Journal: PLoS ONE

    Article Title: Quantitative Analysis of Microbicide Concentrations in Fluids, Gels and Tissues Using Confocal Raman Spectroscopy

    doi: 10.1371/journal.pone.0085124

    Figure Lengend Snippet: Tenofovir concentrations vs. time in tissue and the top fluid compartment in the Transwell assay . Transwell assay experiments were performed to characterize the transport of Tenofovir into and through tissue after microbicide application. The Transwell setup was maintained at 37°C and 5% CO 2 in a Heracell incubator for different amounts of time, ranging from 30 min to 6 h. After incubation, the tissue (7-mm diameter, 1-mm thickness) and fluids were isolated and stored at −80°C. Thawed tissue specimens and fluids were scanned. Quantification of Tenofovir concentrations was performed by interpolation, referencing to calibration curves of Tenofovir in tissue homogenates and fluids (shown in Figure 4 ). Each data point gives the median, and the smallest and largest measurements in the 3 independent experiments (n=3).

    Article Snippet: Freshly excised tissue specimens (7-mm diameter; 1-mm thickness; n=3) were incubated while fully submerged in 1% Tenofovir clinical gel and maintained at 5% CO2 and 37 °C for different amounts of time (2, 6, 9, and 24 h) in a Heracell incubator (Thermo Scientific, Asheville, NC).

    Techniques: Transwell Assay, Incubation, Isolation