incb 18424 jak i calbiochem  (Millipore)

 
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    Name:
    PP242 hydrate
    Description:

    Catalog Number:
    P0037
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    Structured Review

    Millipore incb 18424 jak i calbiochem
    PP242 hydrate

    https://www.bioz.com/result/incb 18424 jak i calbiochem/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    incb 18424 jak i calbiochem - by Bioz Stars, 2021-04
    93/100 stars

    Images

    1) Product Images from "BCL-2 IS A THERAPEUTIC TARGET FOR HYPODIPLOID B-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA"

    Article Title: BCL-2 IS A THERAPEUTIC TARGET FOR HYPODIPLOID B-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA

    Journal: Cancer research

    doi: 10.1158/0008-5472.CAN-18-0236

    Integrated biochemical characterization with viability/survival assays in hypodiploid ALL: A) Cytobank heatmap representing a signaling profiling panel of phospho- and total protein levels (columns) in individually xenografted leukemias from LH and NH subtypes (rows). Levels of phospho- and total proteins were normalized to healthy donor control cells (HM ctrl), yellow indicating increased levels and blue indicating decreased levels. (B and C) NALM-16 cells were cultured for 24h in the presence of increasing concentrations of inhibitors (PP2, INCB-18424, GDC-0941, PP-242, PD-0325901, ABT-263) and effects were measured using (B) cell proliferation and (C) apoptosis levels using ATP-luminescence (Cell Titer Glo) or Caspase 3/7 activation assays, respectively.
    Figure Legend Snippet: Integrated biochemical characterization with viability/survival assays in hypodiploid ALL: A) Cytobank heatmap representing a signaling profiling panel of phospho- and total protein levels (columns) in individually xenografted leukemias from LH and NH subtypes (rows). Levels of phospho- and total proteins were normalized to healthy donor control cells (HM ctrl), yellow indicating increased levels and blue indicating decreased levels. (B and C) NALM-16 cells were cultured for 24h in the presence of increasing concentrations of inhibitors (PP2, INCB-18424, GDC-0941, PP-242, PD-0325901, ABT-263) and effects were measured using (B) cell proliferation and (C) apoptosis levels using ATP-luminescence (Cell Titer Glo) or Caspase 3/7 activation assays, respectively.

    Techniques Used: Cell Culture, Activation Assay

    Related Articles

    Stable Transfection:

    Article Title: Antioxidant Treatment and Induction of Autophagy Cooperate to Reduce Desmin Aggregation in a Cellular Model of Desminopathy
    Article Snippet: .. Statistical analysis with a non-parametric test revealed significant differences indicated by an asterisk (p < 0.05), and an asterisk above the horizontal bar indicates a significant difference between double and single treatments (p < 0.05). (B) C2C12 cells stably transfected and expressing the myc-Desmin D399Y mutant construct under a Doxycycline-inducible promoter (Tet-ON system), were treated with DMSO, PP242, NSC23766, Trolox or a combination of these products, in the presence of Doxycycline for 16 h. Cells were then subjected to heat-shock at 42°C for 2 h (HS), the medium was changed, and cells were incubated in normal growth medium for 24 h. Cells were then fixed and aggregates were counted under a microscope (n = 400 total cells in each 3 independent experiments–for "PP242 + Trolox", 1 experiment). ..

    Transfection:

    Article Title: Antioxidant Treatment and Induction of Autophagy Cooperate to Reduce Desmin Aggregation in a Cellular Model of Desminopathy
    Article Snippet: .. Statistical analysis with a non-parametric test revealed significant differences indicated by an asterisk (p < 0.05), and an asterisk above the horizontal bar indicates a significant difference between double and single treatments (p < 0.05). (B) C2C12 cells stably transfected and expressing the myc-Desmin D399Y mutant construct under a Doxycycline-inducible promoter (Tet-ON system), were treated with DMSO, PP242, NSC23766, Trolox or a combination of these products, in the presence of Doxycycline for 16 h. Cells were then subjected to heat-shock at 42°C for 2 h (HS), the medium was changed, and cells were incubated in normal growth medium for 24 h. Cells were then fixed and aggregates were counted under a microscope (n = 400 total cells in each 3 independent experiments–for "PP242 + Trolox", 1 experiment). ..

    Expressing:

    Article Title: Antioxidant Treatment and Induction of Autophagy Cooperate to Reduce Desmin Aggregation in a Cellular Model of Desminopathy
    Article Snippet: .. Statistical analysis with a non-parametric test revealed significant differences indicated by an asterisk (p < 0.05), and an asterisk above the horizontal bar indicates a significant difference between double and single treatments (p < 0.05). (B) C2C12 cells stably transfected and expressing the myc-Desmin D399Y mutant construct under a Doxycycline-inducible promoter (Tet-ON system), were treated with DMSO, PP242, NSC23766, Trolox or a combination of these products, in the presence of Doxycycline for 16 h. Cells were then subjected to heat-shock at 42°C for 2 h (HS), the medium was changed, and cells were incubated in normal growth medium for 24 h. Cells were then fixed and aggregates were counted under a microscope (n = 400 total cells in each 3 independent experiments–for "PP242 + Trolox", 1 experiment). ..

    Article Title: Antioxidant Treatment and Induction of Autophagy Cooperate to Reduce Desmin Aggregation in a Cellular Model of Desminopathy
    Article Snippet: .. No specific toxicity of PP242 in muscular cells expressing desmin mutants. .. C2C12 cells were transfected with GFP-Desmin D399Y for 4 h, washed, and treated for 16 h with PP242 (10 μM).

    Mutagenesis:

    Article Title: Antioxidant Treatment and Induction of Autophagy Cooperate to Reduce Desmin Aggregation in a Cellular Model of Desminopathy
    Article Snippet: .. Statistical analysis with a non-parametric test revealed significant differences indicated by an asterisk (p < 0.05), and an asterisk above the horizontal bar indicates a significant difference between double and single treatments (p < 0.05). (B) C2C12 cells stably transfected and expressing the myc-Desmin D399Y mutant construct under a Doxycycline-inducible promoter (Tet-ON system), were treated with DMSO, PP242, NSC23766, Trolox or a combination of these products, in the presence of Doxycycline for 16 h. Cells were then subjected to heat-shock at 42°C for 2 h (HS), the medium was changed, and cells were incubated in normal growth medium for 24 h. Cells were then fixed and aggregates were counted under a microscope (n = 400 total cells in each 3 independent experiments–for "PP242 + Trolox", 1 experiment). ..

    Construct:

    Article Title: Antioxidant Treatment and Induction of Autophagy Cooperate to Reduce Desmin Aggregation in a Cellular Model of Desminopathy
    Article Snippet: .. Statistical analysis with a non-parametric test revealed significant differences indicated by an asterisk (p < 0.05), and an asterisk above the horizontal bar indicates a significant difference between double and single treatments (p < 0.05). (B) C2C12 cells stably transfected and expressing the myc-Desmin D399Y mutant construct under a Doxycycline-inducible promoter (Tet-ON system), were treated with DMSO, PP242, NSC23766, Trolox or a combination of these products, in the presence of Doxycycline for 16 h. Cells were then subjected to heat-shock at 42°C for 2 h (HS), the medium was changed, and cells were incubated in normal growth medium for 24 h. Cells were then fixed and aggregates were counted under a microscope (n = 400 total cells in each 3 independent experiments–for "PP242 + Trolox", 1 experiment). ..

    Incubation:

    Article Title: Antioxidant Treatment and Induction of Autophagy Cooperate to Reduce Desmin Aggregation in a Cellular Model of Desminopathy
    Article Snippet: .. Statistical analysis with a non-parametric test revealed significant differences indicated by an asterisk (p < 0.05), and an asterisk above the horizontal bar indicates a significant difference between double and single treatments (p < 0.05). (B) C2C12 cells stably transfected and expressing the myc-Desmin D399Y mutant construct under a Doxycycline-inducible promoter (Tet-ON system), were treated with DMSO, PP242, NSC23766, Trolox or a combination of these products, in the presence of Doxycycline for 16 h. Cells were then subjected to heat-shock at 42°C for 2 h (HS), the medium was changed, and cells were incubated in normal growth medium for 24 h. Cells were then fixed and aggregates were counted under a microscope (n = 400 total cells in each 3 independent experiments–for "PP242 + Trolox", 1 experiment). ..

    Microscopy:

    Article Title: Antioxidant Treatment and Induction of Autophagy Cooperate to Reduce Desmin Aggregation in a Cellular Model of Desminopathy
    Article Snippet: .. Statistical analysis with a non-parametric test revealed significant differences indicated by an asterisk (p < 0.05), and an asterisk above the horizontal bar indicates a significant difference between double and single treatments (p < 0.05). (B) C2C12 cells stably transfected and expressing the myc-Desmin D399Y mutant construct under a Doxycycline-inducible promoter (Tet-ON system), were treated with DMSO, PP242, NSC23766, Trolox or a combination of these products, in the presence of Doxycycline for 16 h. Cells were then subjected to heat-shock at 42°C for 2 h (HS), the medium was changed, and cells were incubated in normal growth medium for 24 h. Cells were then fixed and aggregates were counted under a microscope (n = 400 total cells in each 3 independent experiments–for "PP242 + Trolox", 1 experiment). ..

    other:

    Article Title: mTORC2 promotes cell survival through c-Myc–dependent up-regulation of E2F1
    Article Snippet: Chemicals and reagents Rapamycin, pp242, and 5-FU were purchased from Sigma-Aldrich.

    Article Title: Metformin inhibits salivary adenocarcinoma growth through cell cycle arrest and apoptosis
    Article Snippet: Metformin and pp242 were purchased from Calbiochem (Darmstadt, Germany).

    Article Title: mTOR signaling regulates central and peripheral circadian clock function
    Article Snippet: Chemicals Rapamycin, Torin1, PP242, and insulin were purchased from Sigma-Aldrich (St. Louis, MO) or Selleck Chemicals (Houston, TX).

    Cell Culture:

    Article Title: eIF4A inhibition circumvents uncontrolled DNA replication mediated by 4E-BP1 loss in pancreatic cancer
    Article Snippet: Polysome profiling and qPCR were carried out as described ( ). .. Briefly, cells were cultured in 15-cm dishes and treated with 2.5 μM PP242 or vehicle (DMSO) for 12 hours. .. Cells were washed twice with cold PBS containing 100 μg/ml cycloheximide, collected, and lysed in a hypotonic lysis buffer (5 mM Tris-HCl [pH 7.5], 2.5 mM MgCl2, 1.5 mM KCl, 100 μg/ml cycloheximide, 2 mM DTT, 0.5% Triton X-100, and 0.5% sodium deoxycholate).

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    Millipore incb 18424 jak i calbiochem
    Integrated biochemical characterization with viability/survival assays in hypodiploid ALL: A) Cytobank heatmap representing a signaling profiling panel of phospho- and total protein levels (columns) in individually xenografted leukemias from LH and NH subtypes (rows). Levels of phospho- and total proteins were normalized to healthy donor control cells (HM ctrl), yellow indicating increased levels and blue indicating decreased levels. (B and C) NALM-16 cells were cultured for 24h in the presence of increasing concentrations of inhibitors (PP2, <t>INCB-18424,</t> GDC-0941, PP-242, PD-0325901, ABT-263) and effects were measured using (B) cell proliferation and (C) apoptosis levels using ATP-luminescence (Cell Titer Glo) or Caspase 3/7 activation assays, respectively.
    Incb 18424 Jak I Calbiochem, supplied by Millipore, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/incb 18424 jak i calbiochem/product/Millipore
    Average 93 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    incb 18424 jak i calbiochem - by Bioz Stars, 2021-04
    93/100 stars
      Buy from Supplier

    Image Search Results


    Integrated biochemical characterization with viability/survival assays in hypodiploid ALL: A) Cytobank heatmap representing a signaling profiling panel of phospho- and total protein levels (columns) in individually xenografted leukemias from LH and NH subtypes (rows). Levels of phospho- and total proteins were normalized to healthy donor control cells (HM ctrl), yellow indicating increased levels and blue indicating decreased levels. (B and C) NALM-16 cells were cultured for 24h in the presence of increasing concentrations of inhibitors (PP2, INCB-18424, GDC-0941, PP-242, PD-0325901, ABT-263) and effects were measured using (B) cell proliferation and (C) apoptosis levels using ATP-luminescence (Cell Titer Glo) or Caspase 3/7 activation assays, respectively.

    Journal: Cancer research

    Article Title: BCL-2 IS A THERAPEUTIC TARGET FOR HYPODIPLOID B-LINEAGE ACUTE LYMPHOBLASTIC LEUKEMIA

    doi: 10.1158/0008-5472.CAN-18-0236

    Figure Lengend Snippet: Integrated biochemical characterization with viability/survival assays in hypodiploid ALL: A) Cytobank heatmap representing a signaling profiling panel of phospho- and total protein levels (columns) in individually xenografted leukemias from LH and NH subtypes (rows). Levels of phospho- and total proteins were normalized to healthy donor control cells (HM ctrl), yellow indicating increased levels and blue indicating decreased levels. (B and C) NALM-16 cells were cultured for 24h in the presence of increasing concentrations of inhibitors (PP2, INCB-18424, GDC-0941, PP-242, PD-0325901, ABT-263) and effects were measured using (B) cell proliferation and (C) apoptosis levels using ATP-luminescence (Cell Titer Glo) or Caspase 3/7 activation assays, respectively.

    Article Snippet: Inhibitors:ABT-199 kindly provided by ABBVIE, ABT-737 (Selleck Chemicals, S1002), PP2 (Calbiochem, 529576), INCB-18424 (JAK-I) Calbiochem (420099), GDC0941(Calbiochem, 509226), PP242 (Calbiochem, 475988), PD0325901 (Calbiochem, 444968), PI-90, PI-103, p110α, p110β, p110δ, (were kindly provided by prof. K. Shokat (UCSF)), AKT VIII (Calbiochem, 124018), Dexamethasone (Calbiochem, 265005), Etoposide (Calbiochem, 341205), Cisplatin (Calbiochem, 232120),.

    Techniques: Cell Culture, Activation Assay