in situ dna fragmentation  (Trevigen)

 
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    Trevigen in situ dna fragmentation
    Estrogen-induced osteoclast apoptosis. Osteoclasts were cultured on dentine slices with 0.1 nM E 2 for 24 h. ( A ) A fluorescence micrograph shows normal and <t>apoptotic</t> osteoclasts ( arrowhead ) attached to a dentine slice in a 24-h culture. ×250. ( B ) The TUNEL assay indicates <t>DNA</t> fragmentation in an apoptotic osteoclast. ×250. ( C ) This micrograph, obtained by electron transmission microscopy, demonstrates the gross morphological changes in an apoptotic osteoclast. Nuclear fragments are indicated by the arrowheads. ×2,000.
    In Situ Dna Fragmentation, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Images

    1) Product Images from "Estrogen Inhibits Bone Resorption by Directly Inducing Apoptosis of the Bone-resorbing Osteoclasts "

    Article Title: Estrogen Inhibits Bone Resorption by Directly Inducing Apoptosis of the Bone-resorbing Osteoclasts

    Journal: The Journal of Experimental Medicine

    doi:

    Estrogen-induced osteoclast apoptosis. Osteoclasts were cultured on dentine slices with 0.1 nM E 2 for 24 h. ( A ) A fluorescence micrograph shows normal and apoptotic osteoclasts ( arrowhead ) attached to a dentine slice in a 24-h culture. ×250. ( B ) The TUNEL assay indicates DNA fragmentation in an apoptotic osteoclast. ×250. ( C ) This micrograph, obtained by electron transmission microscopy, demonstrates the gross morphological changes in an apoptotic osteoclast. Nuclear fragments are indicated by the arrowheads. ×2,000.
    Figure Legend Snippet: Estrogen-induced osteoclast apoptosis. Osteoclasts were cultured on dentine slices with 0.1 nM E 2 for 24 h. ( A ) A fluorescence micrograph shows normal and apoptotic osteoclasts ( arrowhead ) attached to a dentine slice in a 24-h culture. ×250. ( B ) The TUNEL assay indicates DNA fragmentation in an apoptotic osteoclast. ×250. ( C ) This micrograph, obtained by electron transmission microscopy, demonstrates the gross morphological changes in an apoptotic osteoclast. Nuclear fragments are indicated by the arrowheads. ×2,000.

    Techniques Used: Cell Culture, Fluorescence, TUNEL Assay, Transmission Assay, Microscopy

    2) Product Images from "The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice"

    Article Title: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice

    Journal: The EMBO Journal

    doi: 10.1093/emboj/19.23.6341

    Fig. 6. MEK1 confers protection from TUNEL, caspase 3 activation and DNA laddering. ( A ) Cultured neonatal cardiomyocytes were infected with Adβgal, AdAkt or AdMEK1 and 24 h later placed in glucose-free and serum-free media in the presence of 2-deoxyglucose to induce apoptosis. The data demonstrate that culturing for 12 or 18 h in the presence of 2-deoxyglucose induced significant TUNEL in Adβgal-infected myocytes, while AdAkt or AdMEK1 infection conferred resistance. ( B ) AdMEK1 and AdAKT also provided partial protection from caspase 3 activation induced by 2-deoxyglucose. ( C ) In vivo , MEK1 transgenic and wild-type mice were subjected to 45 min of cardiac ischemia followed by 4 h of reperfusion (I/R). Forty micrograms of genomic DNA were size fractionated on a 1.4% agarose gel containing ethidium bromide to qualitatively analyze DNA laddering in the heart. MEK1 transgenic hearts were partially resistant to DNA laddering following the I/R procedure compared with sham hearts. Wild-type–sham, n = 6; wild-type–I/R, n = 11; MEK1-TG–sham, n = 8; MEK1-TG–I/R, n = 13. * P
    Figure Legend Snippet: Fig. 6. MEK1 confers protection from TUNEL, caspase 3 activation and DNA laddering. ( A ) Cultured neonatal cardiomyocytes were infected with Adβgal, AdAkt or AdMEK1 and 24 h later placed in glucose-free and serum-free media in the presence of 2-deoxyglucose to induce apoptosis. The data demonstrate that culturing for 12 or 18 h in the presence of 2-deoxyglucose induced significant TUNEL in Adβgal-infected myocytes, while AdAkt or AdMEK1 infection conferred resistance. ( B ) AdMEK1 and AdAKT also provided partial protection from caspase 3 activation induced by 2-deoxyglucose. ( C ) In vivo , MEK1 transgenic and wild-type mice were subjected to 45 min of cardiac ischemia followed by 4 h of reperfusion (I/R). Forty micrograms of genomic DNA were size fractionated on a 1.4% agarose gel containing ethidium bromide to qualitatively analyze DNA laddering in the heart. MEK1 transgenic hearts were partially resistant to DNA laddering following the I/R procedure compared with sham hearts. Wild-type–sham, n = 6; wild-type–I/R, n = 11; MEK1-TG–sham, n = 8; MEK1-TG–I/R, n = 13. * P

    Techniques Used: TUNEL Assay, Activation Assay, DNA Laddering, Cell Culture, Infection, In Vivo, Transgenic Assay, Mouse Assay, Agarose Gel Electrophoresis

    Related Articles

    In Situ:

    Article Title: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice
    Article Snippet: .. In situ DNA fragmentation was detected using TUNEL in 7 µm paraffin-embedded tissue sections or in fixed cultured cardiomyocytes using the CardioTACSTM kit from Trevigen (Gaithersburg, MD) according to the manufacturer’s instructions. .. After the TUNEL procedure, labeled nuclei were visualized by FITC–Extra avidin (Sigma) incubation (1:400 dilution).

    Article Title: Estrogen Inhibits Bone Resorption by Directly Inducing Apoptosis of the Bone-resorbing Osteoclasts
    Article Snippet: .. To detect in situ DNA fragmentation of apoptotic osteoclasts, we employed the TUNEL (TdT-mediated dUTP-biotin nick end-labeling) assay by using a TACS Blue Label™ kit (Trevigen, Inc., Gaithersburg, MD) according to the procedure recommended by the manufacturer. ..

    Article Title: Flucytosine and Amphotericin B Coadministration Induces Dose-Related Renal Injury
    Article Snippet: .. In Situ Detection of DNA Fragmentation In Situ Apoptosis Detection Kit (Trevigen) was used to assess in situ nuclear DNA fragmentation by terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL). .. The incorporation of biotinylated nucleotides allows chromosomal DNA fragmentation to be visualized by binding streptavidin–horseradish peroxidase followed by reaction with a peroxidase substrate that produces a stable, insoluble blue reaction product at the site of peroxidase activity (TACS) Blue Label to generate a dark blue precipitate.

    Article Title: Ductal Injection Does Not Increase the Islet Yield or Function after Cold Storage in a Vascular Perfusion Model
    Article Snippet: .. DNA fragmentation in situ was assessed by the Apoptosis Detection Kit (TREVIGEN Inc., MD, USA). .. Slides (six sections per pancreas and three pancreases per group) were subjected to the TUNEL assay, and evaluated with regard to islets and exocrine tissues.

    Article Title: Regulation of Human Immunodeficiency Virus Replication by 2?,5?-Oligoadenylate-Dependent RNase L
    Article Snippet: The blots were stripped with 0.1× SSC for 10 to 15 min at 100°C and probed with a randomly primed, 32 P-labeled DNA probe of 18S rRNA (American Type Culture Collection). .. DNA fragmentation in situ was measured with the Trevigen Apoptotic Cell System by using terminal deoxynucleotide transferase (15 U) in 0.05 M Tris (pH 7.5)–5 mM MgCl2 –0.6 mM β-mercaptoethanesulfonic acid–50 μg of bovine serum albumin–0.25 mM biotinylated nucleotides (dNTPs) at 37°C for 60 min. ..

    Article Title: HIV-1 Tat and Morphine Have Interactive Effects on Oligodendrocyte Survival and Morphology
    Article Snippet: Anti-caspase-3 was visualized with goat anti-rabbit IgG conjugated to CY-3 (Jackson ImmunoResearch, West Grove, PA). .. In other sections, APC staining was combined with TUNEL labeling to detect in situ DNA fragmentation using the TACS2-TdT Apoptosis Detection Kit (Trevigen, Gaithersburg, MD), per manufacturer's directions. .. Lastly, immunostained sections were incubated with Hoechst 33342 dye (1:20,000) for identification of all nuclei (Molecular Probes), mounted in ProLong Gold antifade reagent (Molecular Probes) and dried for 8 hrs in the dark.

    TUNEL Assay:

    Article Title: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice
    Article Snippet: .. In situ DNA fragmentation was detected using TUNEL in 7 µm paraffin-embedded tissue sections or in fixed cultured cardiomyocytes using the CardioTACSTM kit from Trevigen (Gaithersburg, MD) according to the manufacturer’s instructions. .. After the TUNEL procedure, labeled nuclei were visualized by FITC–Extra avidin (Sigma) incubation (1:400 dilution).

    Article Title: Estrogen Inhibits Bone Resorption by Directly Inducing Apoptosis of the Bone-resorbing Osteoclasts
    Article Snippet: .. To detect in situ DNA fragmentation of apoptotic osteoclasts, we employed the TUNEL (TdT-mediated dUTP-biotin nick end-labeling) assay by using a TACS Blue Label™ kit (Trevigen, Inc., Gaithersburg, MD) according to the procedure recommended by the manufacturer. ..

    Article Title: Flucytosine and Amphotericin B Coadministration Induces Dose-Related Renal Injury
    Article Snippet: .. In Situ Detection of DNA Fragmentation In Situ Apoptosis Detection Kit (Trevigen) was used to assess in situ nuclear DNA fragmentation by terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL). .. The incorporation of biotinylated nucleotides allows chromosomal DNA fragmentation to be visualized by binding streptavidin–horseradish peroxidase followed by reaction with a peroxidase substrate that produces a stable, insoluble blue reaction product at the site of peroxidase activity (TACS) Blue Label to generate a dark blue precipitate.

    Article Title: HIV-1 Tat and Morphine Have Interactive Effects on Oligodendrocyte Survival and Morphology
    Article Snippet: Anti-caspase-3 was visualized with goat anti-rabbit IgG conjugated to CY-3 (Jackson ImmunoResearch, West Grove, PA). .. In other sections, APC staining was combined with TUNEL labeling to detect in situ DNA fragmentation using the TACS2-TdT Apoptosis Detection Kit (Trevigen, Gaithersburg, MD), per manufacturer's directions. .. Lastly, immunostained sections were incubated with Hoechst 33342 dye (1:20,000) for identification of all nuclei (Molecular Probes), mounted in ProLong Gold antifade reagent (Molecular Probes) and dried for 8 hrs in the dark.

    Cell Culture:

    Article Title: The MEK1-ERK1/2 signaling pathway promotes compensated cardiac hypertrophy in transgenic mice
    Article Snippet: .. In situ DNA fragmentation was detected using TUNEL in 7 µm paraffin-embedded tissue sections or in fixed cultured cardiomyocytes using the CardioTACSTM kit from Trevigen (Gaithersburg, MD) according to the manufacturer’s instructions. .. After the TUNEL procedure, labeled nuclei were visualized by FITC–Extra avidin (Sigma) incubation (1:400 dilution).

    End Labeling:

    Article Title: Estrogen Inhibits Bone Resorption by Directly Inducing Apoptosis of the Bone-resorbing Osteoclasts
    Article Snippet: .. To detect in situ DNA fragmentation of apoptotic osteoclasts, we employed the TUNEL (TdT-mediated dUTP-biotin nick end-labeling) assay by using a TACS Blue Label™ kit (Trevigen, Inc., Gaithersburg, MD) according to the procedure recommended by the manufacturer. ..

    Article Title: Flucytosine and Amphotericin B Coadministration Induces Dose-Related Renal Injury
    Article Snippet: .. In Situ Detection of DNA Fragmentation In Situ Apoptosis Detection Kit (Trevigen) was used to assess in situ nuclear DNA fragmentation by terminal deoxy-nucleotidyl transferase (TdT)-mediated dUTP nick end-labeling (TUNEL). .. The incorporation of biotinylated nucleotides allows chromosomal DNA fragmentation to be visualized by binding streptavidin–horseradish peroxidase followed by reaction with a peroxidase substrate that produces a stable, insoluble blue reaction product at the site of peroxidase activity (TACS) Blue Label to generate a dark blue precipitate.

    Staining:

    Article Title: HIV-1 Tat and Morphine Have Interactive Effects on Oligodendrocyte Survival and Morphology
    Article Snippet: Anti-caspase-3 was visualized with goat anti-rabbit IgG conjugated to CY-3 (Jackson ImmunoResearch, West Grove, PA). .. In other sections, APC staining was combined with TUNEL labeling to detect in situ DNA fragmentation using the TACS2-TdT Apoptosis Detection Kit (Trevigen, Gaithersburg, MD), per manufacturer's directions. .. Lastly, immunostained sections were incubated with Hoechst 33342 dye (1:20,000) for identification of all nuclei (Molecular Probes), mounted in ProLong Gold antifade reagent (Molecular Probes) and dried for 8 hrs in the dark.

    Labeling:

    Article Title: HIV-1 Tat and Morphine Have Interactive Effects on Oligodendrocyte Survival and Morphology
    Article Snippet: Anti-caspase-3 was visualized with goat anti-rabbit IgG conjugated to CY-3 (Jackson ImmunoResearch, West Grove, PA). .. In other sections, APC staining was combined with TUNEL labeling to detect in situ DNA fragmentation using the TACS2-TdT Apoptosis Detection Kit (Trevigen, Gaithersburg, MD), per manufacturer's directions. .. Lastly, immunostained sections were incubated with Hoechst 33342 dye (1:20,000) for identification of all nuclei (Molecular Probes), mounted in ProLong Gold antifade reagent (Molecular Probes) and dried for 8 hrs in the dark.

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    • tunel assay dna fragmentation  (Trevigen)
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    Trevigen tunel assay dna fragmentation
    Apoptotic effects of BT-cisplatin combination on isogenic pairs of ovarian cancer cell lines. Representative images of Hoechst 33258 staining of A2780 and A2780-CDDP ( a ) or IGROV and IGROV-1-CDDP ( c ) cells treated with BT and/or cisplatin as indicated. Percent of apoptosis in terms of <t>DNA</t> fragmentation (quantified via <t>TUNEL</t> assay) are shown for A2780 and A2780-CDDP ( b ) or IGROV and IGROV-1-CDDP ( d ) cells treated with BT or cisplatin alone or in combination. Data were expressed as means ± SD of duplicate experiments. Comparisons between cisplatin alone treated and combination treated for each cell line were performed using Student’s t–test. Asterisks (*) indicate p
    Tunel Assay Dna Fragmentation, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/tunel assay dna fragmentation/product/Trevigen
    Average 86 stars, based on 1 article reviews
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    in situ dna fragmentation  (Trevigen)
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    Trevigen in situ dna fragmentation
    Estrogen-induced osteoclast apoptosis. Osteoclasts were cultured on dentine slices with 0.1 nM E 2 for 24 h. ( A ) A fluorescence micrograph shows normal and <t>apoptotic</t> osteoclasts ( arrowhead ) attached to a dentine slice in a 24-h culture. ×250. ( B ) The TUNEL assay indicates <t>DNA</t> fragmentation in an apoptotic osteoclast. ×250. ( C ) This micrograph, obtained by electron transmission microscopy, demonstrates the gross morphological changes in an apoptotic osteoclast. Nuclear fragments are indicated by the arrowheads. ×2,000.
    In Situ Dna Fragmentation, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in situ dna fragmentation/product/Trevigen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in situ dna fragmentation - by Bioz Stars, 2021-04
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    dna fragmentation  (Trevigen)
    86
    Trevigen dna fragmentation
    Immunoelectron microscopic characterization of apoptosis at the rupture sites. A: Ultrastructural examination of the macrophages ( arrows ) at the rupture site showed that the majority had nuclear changes suggestive of apoptosis. B: Example demonstrating varying stages of nuclear shrinkage with condensation of chromatin at the nuclear periphery. The presence of lipid globules and lack of contractile elements help identify the macrophages. ISEL was performed on ultra-thin sections with <t>TdT</t> and biotinylated nucleotides. Fragmented <t>DNA</t> was visualized using a 10-nm gold, streptavidin conjugate. C: High-power electron micrograph of the region indicated by the inset in B , note the gold particles are selectively localized on the condensed chromatin material.
    Dna Fragmentation, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dna fragmentation/product/Trevigen
    Average 86 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dna fragmentation - by Bioz Stars, 2021-04
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    apoptotic cell system  (Trevigen)
    86
    Trevigen apoptotic cell system
    Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of <t>apoptotic</t> cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.
    Apoptotic Cell System, supplied by Trevigen, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/apoptotic cell system/product/Trevigen
    Average 86 stars, based on 1 article reviews
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    apoptotic cell system - by Bioz Stars, 2021-04
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    Image Search Results


    Apoptotic effects of BT-cisplatin combination on isogenic pairs of ovarian cancer cell lines. Representative images of Hoechst 33258 staining of A2780 and A2780-CDDP ( a ) or IGROV and IGROV-1-CDDP ( c ) cells treated with BT and/or cisplatin as indicated. Percent of apoptosis in terms of DNA fragmentation (quantified via TUNEL assay) are shown for A2780 and A2780-CDDP ( b ) or IGROV and IGROV-1-CDDP ( d ) cells treated with BT or cisplatin alone or in combination. Data were expressed as means ± SD of duplicate experiments. Comparisons between cisplatin alone treated and combination treated for each cell line were performed using Student’s t–test. Asterisks (*) indicate p

    Journal: BMC Cancer

    Article Title: Evaluation of the cytotoxicity of the Bithionol - cisplatin combination in a panel of human ovarian cancer cell lines

    doi: 10.1186/s12885-016-3034-2

    Figure Lengend Snippet: Apoptotic effects of BT-cisplatin combination on isogenic pairs of ovarian cancer cell lines. Representative images of Hoechst 33258 staining of A2780 and A2780-CDDP ( a ) or IGROV and IGROV-1-CDDP ( c ) cells treated with BT and/or cisplatin as indicated. Percent of apoptosis in terms of DNA fragmentation (quantified via TUNEL assay) are shown for A2780 and A2780-CDDP ( b ) or IGROV and IGROV-1-CDDP ( d ) cells treated with BT or cisplatin alone or in combination. Data were expressed as means ± SD of duplicate experiments. Comparisons between cisplatin alone treated and combination treated for each cell line were performed using Student’s t–test. Asterisks (*) indicate p

    Article Snippet: Apoptosis quantification via TUNEL assay DNA fragmentation was detected using the TiterTACS® 2 TdT in Situ Colorimetric Apoptosis Detection Kit (Trevigen, Gaithersburg, MD) following the manufacturer’s instructions.

    Techniques: Staining, TUNEL Assay

    Estrogen-induced osteoclast apoptosis. Osteoclasts were cultured on dentine slices with 0.1 nM E 2 for 24 h. ( A ) A fluorescence micrograph shows normal and apoptotic osteoclasts ( arrowhead ) attached to a dentine slice in a 24-h culture. ×250. ( B ) The TUNEL assay indicates DNA fragmentation in an apoptotic osteoclast. ×250. ( C ) This micrograph, obtained by electron transmission microscopy, demonstrates the gross morphological changes in an apoptotic osteoclast. Nuclear fragments are indicated by the arrowheads. ×2,000.

    Journal: The Journal of Experimental Medicine

    Article Title: Estrogen Inhibits Bone Resorption by Directly Inducing Apoptosis of the Bone-resorbing Osteoclasts

    doi:

    Figure Lengend Snippet: Estrogen-induced osteoclast apoptosis. Osteoclasts were cultured on dentine slices with 0.1 nM E 2 for 24 h. ( A ) A fluorescence micrograph shows normal and apoptotic osteoclasts ( arrowhead ) attached to a dentine slice in a 24-h culture. ×250. ( B ) The TUNEL assay indicates DNA fragmentation in an apoptotic osteoclast. ×250. ( C ) This micrograph, obtained by electron transmission microscopy, demonstrates the gross morphological changes in an apoptotic osteoclast. Nuclear fragments are indicated by the arrowheads. ×2,000.

    Article Snippet: To detect in situ DNA fragmentation of apoptotic osteoclasts, we employed the TUNEL (TdT-mediated dUTP-biotin nick end-labeling) assay by using a TACS Blue Label™ kit (Trevigen, Inc., Gaithersburg, MD) according to the procedure recommended by the manufacturer.

    Techniques: Cell Culture, Fluorescence, TUNEL Assay, Transmission Assay, Microscopy

    Immunoelectron microscopic characterization of apoptosis at the rupture sites. A: Ultrastructural examination of the macrophages ( arrows ) at the rupture site showed that the majority had nuclear changes suggestive of apoptosis. B: Example demonstrating varying stages of nuclear shrinkage with condensation of chromatin at the nuclear periphery. The presence of lipid globules and lack of contractile elements help identify the macrophages. ISEL was performed on ultra-thin sections with TdT and biotinylated nucleotides. Fragmented DNA was visualized using a 10-nm gold, streptavidin conjugate. C: High-power electron micrograph of the region indicated by the inset in B , note the gold particles are selectively localized on the condensed chromatin material.

    Journal: The American Journal of Pathology

    Article Title: Localization of Apoptotic Macrophages at the Site of Plaque Rupture in Sudden Coronary Death

    doi:

    Figure Lengend Snippet: Immunoelectron microscopic characterization of apoptosis at the rupture sites. A: Ultrastructural examination of the macrophages ( arrows ) at the rupture site showed that the majority had nuclear changes suggestive of apoptosis. B: Example demonstrating varying stages of nuclear shrinkage with condensation of chromatin at the nuclear periphery. The presence of lipid globules and lack of contractile elements help identify the macrophages. ISEL was performed on ultra-thin sections with TdT and biotinylated nucleotides. Fragmented DNA was visualized using a 10-nm gold, streptavidin conjugate. C: High-power electron micrograph of the region indicated by the inset in B , note the gold particles are selectively localized on the condensed chromatin material.

    Article Snippet: In situ labeling of DNA fragmentation was performed using terminal deoxyribonucleotide transferase (TdT)-mediated nick end labeling based on an in situ apoptosis detection kit (TACS; Trevigen, Gaithersburg, MD).

    Techniques:

    Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of apoptotic cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.

    Journal: Journal of Virology

    Article Title: Regulation of Human Immunodeficiency Virus Replication by 2?,5?-Oligoadenylate-Dependent RNase L

    doi:

    Figure Lengend Snippet: Overexpression of RNase L from NL4-3 recombinant proviral DNA (NL4-3/sRL) accelerates HIV-induced cell death. Jurkat cells were transfected with pNL4-3 (○), pNL4-3/Δ nef (◊), pNL4-3/aRL (▴), or pNL4-3/sRL (•) or were mock transfected with salmon sperm DNA (□), and the percentage of apoptotic cells as a function of time was determined by in situ detection of DNA fragmentation. d, days.

    Article Snippet: DNA fragmentation in situ was measured with the Trevigen Apoptotic Cell System by using terminal deoxynucleotide transferase (15 U) in 0.05 M Tris (pH 7.5)–5 mM MgCl2 –0.6 mM β-mercaptoethanesulfonic acid–50 μg of bovine serum albumin–0.25 mM biotinylated nucleotides (dNTPs) at 37°C for 60 min.

    Techniques: Over Expression, Recombinant, Transfection, In Situ