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TaKaRa in fusion hd ecodry cloning kit
In Fusion Hd Ecodry Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 10 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/in fusion hd ecodry cloning kit/product/TaKaRa
Average 99 stars, based on 10 article reviews
Price from $9.99 to $1999.99
in fusion hd ecodry cloning kit - by Bioz Stars, 2020-04
99/100 stars

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Clone Assay:

Article Title: The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites, et al. The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites
Article Snippet: .. The PfBiP‐Ty1‐KDEL‐KDEL PCR was cloned into the pCEN‐DHOD vector cut with Nhe1 and BglII (New England Biolabs) using the IN‐Fusion HD EcoDry Cloning Kit (Clontech). .. 4.2 Cell culture, transfections, and isolation of clonal cell lines Parasites were grown in RPMI 1640 media supplemented with Albumax 1 (Gibco) and transfected as described previously (Beck et al., ; Cobb et al., ; Florentin et al., ; Muralidharan et al., ; Muralidharan et al., ).

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: .. Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations. .. Briefly, 10–50 ng of purified PCR product (2 μL postelution) was mixed with 50 ng of linearized pGL4.23 vector in a total volume of 10 μL, added to the In-Fusion HD EcoDry pellet, and mixed by pipetting.

Amplification:

Article Title: The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites, et al. The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites
Article Snippet: PfBiP was then amplified from the cDNA using primers P14 and P15. .. The PfBiP‐Ty1‐KDEL‐KDEL PCR was cloned into the pCEN‐DHOD vector cut with Nhe1 and BglII (New England Biolabs) using the IN‐Fusion HD EcoDry Cloning Kit (Clontech).

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: Paragraph title: Amplification and Cloning. ... Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations.

Nucleic Acid Electrophoresis:

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: Thermal cycling was performed as follows: initial denaturation at 98 °C for 1 min, 35 cycles of denaturation at 98 °C for 10 s, annealing at 65 °C for 15 s, extension at 72 °C for 20 s, and final extension at 72 °C for 5 min. An aliquot (5 μL) of PCR product was visualized by 2% agarose TAE (Tris base, acetic acid, and EDTA) gel electrophoresis. .. Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations.

Magnetic Beads:

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: PCR products were purified using AMPure XP magnetic beads (Beckman Coulter) following the manufacturer’s recommendations, and the final elution was performed using 30 μL of 10 mM Tris pH 8.0. .. Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations.

Purification:

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: .. Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations. .. Briefly, 10–50 ng of purified PCR product (2 μL postelution) was mixed with 50 ng of linearized pGL4.23 vector in a total volume of 10 μL, added to the In-Fusion HD EcoDry pellet, and mixed by pipetting.

Polymerase Chain Reaction:

Article Title: The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites, et al. The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites
Article Snippet: .. The PfBiP‐Ty1‐KDEL‐KDEL PCR was cloned into the pCEN‐DHOD vector cut with Nhe1 and BglII (New England Biolabs) using the IN‐Fusion HD EcoDry Cloning Kit (Clontech). .. 4.2 Cell culture, transfections, and isolation of clonal cell lines Parasites were grown in RPMI 1640 media supplemented with Albumax 1 (Gibco) and transfected as described previously (Beck et al., ; Cobb et al., ; Florentin et al., ; Muralidharan et al., ; Muralidharan et al., ).

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: .. Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations. .. Briefly, 10–50 ng of purified PCR product (2 μL postelution) was mixed with 50 ng of linearized pGL4.23 vector in a total volume of 10 μL, added to the In-Fusion HD EcoDry pellet, and mixed by pipetting.

Incubation:

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations. .. The In-Fusion reaction mixture was incubated at 37 °C for 15 min, followed by 50 °C for 15 min, and was then cooled on ice.

Selection:

Article Title: The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites, et al. The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites
Article Snippet: The pCEN vector was modified to contain the DHOD resistance gene instead of the DHFR for parasite selection (Iwanaga et al., ). .. The PfBiP‐Ty1‐KDEL‐KDEL PCR was cloned into the pCEN‐DHOD vector cut with Nhe1 and BglII (New England Biolabs) using the IN‐Fusion HD EcoDry Cloning Kit (Clontech).

Modification:

Article Title: The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites, et al. The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites
Article Snippet: The pCEN vector was modified to contain the DHOD resistance gene instead of the DHFR for parasite selection (Iwanaga et al., ). .. The PfBiP‐Ty1‐KDEL‐KDEL PCR was cloned into the pCEN‐DHOD vector cut with Nhe1 and BglII (New England Biolabs) using the IN‐Fusion HD EcoDry Cloning Kit (Clontech).

Gel Extraction:

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: The pGL4.23 vector (Promega) was linearized by double digestion with KpnI-HF (New England Biolabs) and XhoI (New England Biolabs) and gel-purified using a QIAquick Gel Extraction Kit (Qiagen). .. Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations.

Transformation Assay:

Article Title: The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites, et al. The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites
Article Snippet: The resulting product was then digested with Nhe1 and BglII and inserted into pCEN‐DHFR (Iwanaga et al., ) that was digested with Nhe1 and BglII (New England Biolabs) using a T4 ligase and transformed into bacteria as described previously (Cobb et al., ; Florentin et al., ). .. The PfBiP‐Ty1‐KDEL‐KDEL PCR was cloned into the pCEN‐DHOD vector cut with Nhe1 and BglII (New England Biolabs) using the IN‐Fusion HD EcoDry Cloning Kit (Clontech).

Variant Assay:

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: Variant-centered amplicons were PCR-amplified using genomic DNA from 1000 Genomes Project samples ( ) that were homozygous for the reference or alternate allele at each variant. .. Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations.

Plasmid Preparation:

Article Title: The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites, et al. The endoplasmic reticulum chaperone PfGRP170 is essential for asexual development and is linked to stress response in malaria parasites
Article Snippet: .. The PfBiP‐Ty1‐KDEL‐KDEL PCR was cloned into the pCEN‐DHOD vector cut with Nhe1 and BglII (New England Biolabs) using the IN‐Fusion HD EcoDry Cloning Kit (Clontech). .. 4.2 Cell culture, transfections, and isolation of clonal cell lines Parasites were grown in RPMI 1640 media supplemented with Albumax 1 (Gibco) and transfected as described previously (Beck et al., ; Cobb et al., ; Florentin et al., ; Muralidharan et al., ; Muralidharan et al., ).

Article Title: INAUGURAL ARTICLE by a Recently Elected Academy Member:Multiple SCN5A variant enhancers modulate its cardiac gene expression and the QT interval
Article Snippet: .. Purified PCR products were cloned into linearized pGL42.3 vector using an In-Fusion HD EcoDry Cloning Kit (Clontech) following the manufacturer’s recommendations. .. Briefly, 10–50 ng of purified PCR product (2 μL postelution) was mixed with 50 ng of linearized pGL4.23 vector in a total volume of 10 μL, added to the In-Fusion HD EcoDry pellet, and mixed by pipetting.

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    TaKaRa in fusion hd ecodry cloning kit
    In Fusion Hd Ecodry Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in fusion hd ecodry cloning kit/product/TaKaRa
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    in fusion hd ecodry cloning kit - by Bioz Stars, 2020-04
    99/100 stars
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