in fusion ecodry hd cloning kit  (TaKaRa)

 
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    Name:
    In Fusion HD EcoDry Cloning Kit
    Description:
    In Fusion Cloning offers a one step high efficiency and high throughput cloning method for projects that require a large number of cloning reactions such as expression screening studies and library construction
    Catalog Number:
    639691
    Price:
    None
    Size:
    96 Rxns
    Category:
    In Fusion Cloning for high throughput workflows In Fusion Cloning Cloning
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    Structured Review

    TaKaRa in fusion ecodry hd cloning kit
    In Fusion Cloning offers a one step high efficiency and high throughput cloning method for projects that require a large number of cloning reactions such as expression screening studies and library construction
    https://www.bioz.com/result/in fusion ecodry hd cloning kit/product/TaKaRa
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    in fusion ecodry hd cloning kit - by Bioz Stars, 2020-04
    99/100 stars

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    Related Articles

    Clone Assay:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech). .. Additional tau 3’-UTR sequences were subcloned from the wild-type human tau 3’-UTR vector using the same method.

    Article Title: Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping
    Article Snippet: .. Production of bacterial artificial chromosomes (BACs) The cDNA amplicons were inserted into the BAC vector pBeloBAC11 (New England BioLabs) using the In-Fusion HD Eco-Dry Cloning Kit (Clontech). .. Briefly, pBeloBAC11 was converted to a linearized form using the long PCR as above, with primers Kos15_NotI_pBelF and Kos15_NotI_pBelR (Additional file ), which contain 15-nt overhangs identical to regions of the primers used for the preparation of the cDNA amplicons.

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: .. The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.). .. The cDNAs encoding the ectodomain of human and mouse MAdCAM-1 proteins were amplified by RT-PCR and fused to a cDNA encoding an IgG Fc fragment into pCDNA3.1.

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech). .. Positive clones were selected on ampicillin/LB agar plate with single colonies picked to amplify plasmids with QIA prep miniprep kit (Qiagen).

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: .. We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Article Title: Biochemical characterization of the Lassa virus L protein
    Article Snippet: .. Cloning, expression, and purification of LASV Z The Z gene of Lassa strain AV was cloned into a pOPINF or pOPINJ vector ( ) using the In-Fusion HD EcoDry Cloning kit. .. The Z protein with N-terminal His6 fusion tag (pOPINF) or N-terminal GST fusion protein (pOPINJ) was expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17 °C overnight using terrific broth medium (supplemented with 100 μm ZnSO4 ) and 0.5 mm isopropyl β-d -thiogalactopyranoside for induction.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. The CT-myc and HA-CT constructs were used as parent plasmids to individually mutate the cysteines at positions 269, 271, and 357 to either serine or alanine.

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: .. The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions. .. Transfections were performed in solution at the same time as seeding, using Viafect (Promega) as a carrier for the I942E-EGFR, L834R-EGFR, T766M-EGFR and wtEGFR plasmids.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan). .. Fifty microgram plasmid of identified positive clone was transiently transfected into 1×106 purified T. gondii according to the description elsewhere .

    Article Title: Potency and Resistance Analysis of Hepatitis C Virus NS5B Polymerase Inhibitor BMS-791325 on All Major Genotypes
    Article Snippet: .. Amplicons were subjected directly to sequence analysis and cloned into the GT1a shuttle replicon for GT3a and the GT1b shuttle replicon for GT2b to -6 using the in-fusion HD EcoDry cloning kit (Clontech, Mountain View, CA, USA). .. In addition, for HCV GT2b, -4a, and -5a, a patient-derived NS5B sequence known to generate a viable chimeric replicon ( ) was synthesized by DNA 2.0 and cloned into the GT1b shuttle replicon.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. The sUL16-HA, sNT-HA, sNT-GFP, and sCT-HA constructs were cloned into the pEGFP-N2 vector by use of an In-Fusion EcoDry cloning kit (Clontech). ..

    Amplification:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: The wild-type human tau 3’-UTR sequence was amplified from genomic DNA derived from primary human neonatal dermal fibroblasts (ATCC) by PCR using KAPA HiFi HotStart ReadyMix (Kapa Biosystems). .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech).

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: .. The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: The cDNAs encoding the full-length human α4, β7, and mouse β7 proteins were amplified by RT-PCR and cloned into pCDNA3.1 or pLVX lentiviral expression vector. .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech). .. Positive clones were selected on ampicillin/LB agar plate with single colonies picked to amplify plasmids with QIA prep miniprep kit (Qiagen).

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: EGFR IIIV/KKRE was amplified by PCR using primers containing infusion tags (described in Supplementary Table ) for the insertion of the PCR product into the pOPINE vector cut with Nco I and Pme I (gift from Prof Ray Owens, University of Oxford). .. The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions.

    Article Title: Potency and Resistance Analysis of Hepatitis C Virus NS5B Polymerase Inhibitor BMS-791325 on All Major Genotypes
    Article Snippet: Patient-derived NS5Bs were amplified by nested PCR with Platinum Taq high-fidelity DNA polymerase (Invitrogen). .. Amplicons were subjected directly to sequence analysis and cloned into the GT1a shuttle replicon for GT3a and the GT1b shuttle replicon for GT2b to -6 using the in-fusion HD EcoDry cloning kit (Clontech, Mountain View, CA, USA).

    Synthesized:

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: 2.2 FGF21 expression and purification The human FGF21 gene sequence was optimized based on E. coli preferred codons and was synthesized by Integrated DNA Technologies. .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech).

    Article Title: Potency and Resistance Analysis of Hepatitis C Virus NS5B Polymerase Inhibitor BMS-791325 on All Major Genotypes
    Article Snippet: Amplicons were subjected directly to sequence analysis and cloned into the GT1a shuttle replicon for GT3a and the GT1b shuttle replicon for GT2b to -6 using the in-fusion HD EcoDry cloning kit (Clontech, Mountain View, CA, USA). .. In addition, for HCV GT2b, -4a, and -5a, a patient-derived NS5B sequence known to generate a viable chimeric replicon ( ) was synthesized by DNA 2.0 and cloned into the GT1b shuttle replicon.

    Construct:

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.). .. Mouse β7 and human talin single guide RNAs (sgRNAs) were constructed in vector pSpCas9n(BB)-2A-Puro (48141; Addgene; ).

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: .. We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. The CT-myc and HA-CT constructs were used as parent plasmids to individually mutate the cysteines at positions 269, 271, and 357 to either serine or alanine.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. The sUL16-HA, sNT-HA, sNT-GFP, and sCT-HA constructs were cloned into the pEGFP-N2 vector by use of an In-Fusion EcoDry cloning kit (Clontech). ..

    Infection:

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: Intracellular T. gondii was prepared by the infection of Vero cells cultured in an eight-well chamber slide for 48 h; the slide was fixed and stained as described above. .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan).

    Article Title: Potency and Resistance Analysis of Hepatitis C Virus NS5B Polymerase Inhibitor BMS-791325 on All Major Genotypes
    Article Snippet: Plasma samples of HCV patients infected with GT2b, -3a, -4a, and -5a were purchased from Boca Biolistics (Coconut Creek, FL, USA), while GT6 patient sera were obtained from SeraCare Life Science (Milford, MA, USA) or provided by Huy Trinh. .. Amplicons were subjected directly to sequence analysis and cloned into the GT1a shuttle replicon for GT3a and the GT1b shuttle replicon for GT2b to -6 using the in-fusion HD EcoDry cloning kit (Clontech, Mountain View, CA, USA).

    Expressing:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech). .. Additional tau 3’-UTR sequences were subcloned from the wild-type human tau 3’-UTR vector using the same method.

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: The cDNAs encoding the full-length human α4, β7, and mouse β7 proteins were amplified by RT-PCR and cloned into pCDNA3.1 or pLVX lentiviral expression vector. .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: Paragraph title: FGF21 expression and purification ... Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech).

    Article Title: Biochemical characterization of the Lassa virus L protein
    Article Snippet: .. Cloning, expression, and purification of LASV Z The Z gene of Lassa strain AV was cloned into a pOPINF or pOPINJ vector ( ) using the In-Fusion HD EcoDry Cloning kit. .. The Z protein with N-terminal His6 fusion tag (pOPINF) or N-terminal GST fusion protein (pOPINJ) was expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17 °C overnight using terrific broth medium (supplemented with 100 μm ZnSO4 ) and 0.5 mm isopropyl β-d -thiogalactopyranoside for induction.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: Paragraph title: Mammalian expression plasmids. ... CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech).

    Modification:

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: A modified pET-15b vector encoding a His6-AviTag at the N terminus of the integrin cytoplasmic tail was used as a recombinant model protein affinity matrix as previously described ( ). cDNA encoding integrin β7 (730–779) cytoplasmic tails, WT, β7(L758A), and β7(Y759A) were cloned into this vector, replacing the αIIb tail sequence, and recombinant model proteins were produced as described previously ( ). .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: LIC-SUMO vector was used as an expression vector, which was modified from pet21b (Novagen) by inserting yeast small ubiquitin-like modifier (SUMO) sequence after 6XHis tag and the deletion of MCS region. .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech).

    Western Blot:

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: The wtEGFR pCDNA3 plasmid used as a control in the western blot experiments was a gift from Prof. Y. Yarden (Weizmann Institute of Science). .. The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: Paragraph title: Anti-rP104-1/GST serum preparation, Western blotting, and immunofluorescence assay (IFA) ... The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan).

    Transformation Assay:

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: .. The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Derivative Assay:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: The wild-type human tau 3’-UTR sequence was amplified from genomic DNA derived from primary human neonatal dermal fibroblasts (ATCC) by PCR using KAPA HiFi HotStart ReadyMix (Kapa Biosystems). .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech).

    Transfection:

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech). .. Positive clones were selected on ampicillin/LB agar plate with single colonies picked to amplify plasmids with QIA prep miniprep kit (Qiagen).

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: Paragraph title: Plasmids and transfections ... The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: As an alternative strategy, we introduced a FLAG tag to the C-terminus of P104 and transfected into RH parasites using pMini.3×Flag.ht. vector under the control of GRA1 promoter . .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan).

    Sequencing:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech). .. Additional tau 3’-UTR sequences were subcloned from the wild-type human tau 3’-UTR vector using the same method.

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: A modified pET-15b vector encoding a His6-AviTag at the N terminus of the integrin cytoplasmic tail was used as a recombinant model protein affinity matrix as previously described ( ). cDNA encoding integrin β7 (730–779) cytoplasmic tails, WT, β7(L758A), and β7(Y759A) were cloned into this vector, replacing the αIIb tail sequence, and recombinant model proteins were produced as described previously ( ). .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: LIC-SUMO vector was used as an expression vector, which was modified from pet21b (Novagen) by inserting yeast small ubiquitin-like modifier (SUMO) sequence after 6XHis tag and the deletion of MCS region. .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech).

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The resulting construct, pEc AG137, was confirmed by sequencing using primers 5′ pJSB275 seq and 3′ pJSB275 seq.

    Article Title: Potency and Resistance Analysis of Hepatitis C Virus NS5B Polymerase Inhibitor BMS-791325 on All Major Genotypes
    Article Snippet: .. Amplicons were subjected directly to sequence analysis and cloned into the GT1a shuttle replicon for GT3a and the GT1b shuttle replicon for GT2b to -6 using the in-fusion HD EcoDry cloning kit (Clontech, Mountain View, CA, USA). .. In addition, for HCV GT2b, -4a, and -5a, a patient-derived NS5B sequence known to generate a viable chimeric replicon ( ) was synthesized by DNA 2.0 and cloned into the GT1b shuttle replicon.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: The sUL16-HA, sNT-HA, sNT-GFP, and sCT-HA constructs were cloned into the pEGFP-N2 vector by use of an In-Fusion EcoDry cloning kit (Clontech). .. In all these plasmids, the UL16 derivatives were tagged with an N-terminal amino acid sequence (GSSKSKPKDL) from the v-Src oncogene ( , , , , ).

    Inverse PCR:

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. The ermB cassette was amplified from pEASY-T1 ermB (primers E.F and E.R) and was ligated into an inverse PCR product of the original construct amplified using primers 2 and 3.

    Cell Culture:

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: Intracellular T. gondii was prepared by the infection of Vero cells cultured in an eight-well chamber slide for 48 h; the slide was fixed and stained as described above. .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan).

    Generated:

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: Insertion-deletion mutants of ATCC 10557 were generated using allelic exchange methods. .. The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech).

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. The CT-myc and HA-CT constructs were used as parent plasmids to individually mutate the cysteines at positions 269, 271, and 357 to either serine or alanine.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: Firstly, genome DNA was extracted from purified T. gondii and subjected to a PCR reaction using the following primers, which generated restriction sites Eco T22I and Bgl II: 5′- AGAAATCAAGCAAGA TGTGGAAGTACGGATTTTTTCTGACAG-3′ (sense) and 5′ CTGGTACCGATATCA GAGAAGGGCAGAAATCCCTGGGAC-3′ (antisense). .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan).

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. The sUL16-HA, sNT-HA, sNT-GFP, and sCT-HA constructs were cloned into the pEGFP-N2 vector by use of an In-Fusion EcoDry cloning kit (Clontech).

    Polymerase Chain Reaction:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: The wild-type human tau 3’-UTR sequence was amplified from genomic DNA derived from primary human neonatal dermal fibroblasts (ATCC) by PCR using KAPA HiFi HotStart ReadyMix (Kapa Biosystems). .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech).

    Article Title: Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping
    Article Snippet: Production of bacterial artificial chromosomes (BACs) The cDNA amplicons were inserted into the BAC vector pBeloBAC11 (New England BioLabs) using the In-Fusion HD Eco-Dry Cloning Kit (Clontech). .. Briefly, pBeloBAC11 was converted to a linearized form using the long PCR as above, with primers Kos15_NotI_pBelF and Kos15_NotI_pBelR (Additional file ), which contain 15-nt overhangs identical to regions of the primers used for the preparation of the cDNA amplicons.

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: EGFR IIIV/KKRE was amplified by PCR using primers containing infusion tags (described in Supplementary Table ) for the insertion of the PCR product into the pOPINE vector cut with Nco I and Pme I (gift from Prof Ray Owens, University of Oxford). .. The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan). .. Fifty microgram plasmid of identified positive clone was transiently transfected into 1×106 purified T. gondii according to the description elsewhere .

    Recombinant:

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: Paragraph title: cDNAs and recombinant proteins ... Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Immunofluorescence:

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: Paragraph title: Anti-rP104-1/GST serum preparation, Western blotting, and immunofluorescence assay (IFA) ... The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan).

    Mutagenesis:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: Paragraph title: Cloning and Site-Directed Mutagenesis ... This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech).

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.). .. The cDNAs encoding the ectodomain of human and mouse MAdCAM-1 proteins were amplified by RT-PCR and fused to a cDNA encoding an IgG Fc fragment into pCDNA3.1.

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: Paragraph title: Construction of Bb AG132, a conditional NBD mutant (see Table S2  for primers). (i) Construction of pEcAG137, a shuttle vector containing an IPTG-inducible NBD. ... We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA).

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. QuikChange mutagenesis was used to incorporate the following single-amino-acid substitutions into the pCT-myc and pCT.C271S backgrounds: T162A, Q165A, E168A, E174A, D176A, V181A, E188A, W200A, Y209A, W211A, D214A, L217A, L230A, W232A, E245A, H252A, D254A, N257A, W260A, T266A, W277A, Q282A, T284A, Q288A, D290A, F298A, D303A, Q309A, R312A, D318A, Q321A, E322A, R327A, D331A, W340A, and F345A. pCT-myc was used as a template for QuikChange deletion mutagenesis to generate the following myc-epitope-tagged constructs: pCT(156–205aa), pCT(156–250aa), pCT(206–373aa), pCT(206–279aa), pCT(250–373aa), pCT(280–373aa), and pCT(326–373aa).

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: Reactions were performed using a site-directed mutagenesis kit from Stratagene following the manufacturer’s instructions. .. The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions.

    Isolation:

    Article Title: Potency and Resistance Analysis of Hepatitis C Virus NS5B Polymerase Inhibitor BMS-791325 on All Major Genotypes
    Article Snippet: RNA was isolated from HCV-infected patient sera using the QIAamp MinElute virus vacuum system according to the manufacturer's instructions. .. Amplicons were subjected directly to sequence analysis and cloned into the GT1a shuttle replicon for GT3a and the GT1b shuttle replicon for GT2b to -6 using the in-fusion HD EcoDry cloning kit (Clontech, Mountain View, CA, USA).

    Purification:

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.). .. Recombinant MAdCAM-1 (Val1 to Pro315) fused to the Fc2/3 regions of human IgG1 was produced in pcDNA3.0 (Invitrogen) and purified by Protein A resin (GenScript) as described previously ( ).

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: Paragraph title: FGF21 expression and purification ... Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech).

    Article Title: Biochemical characterization of the Lassa virus L protein
    Article Snippet: .. Cloning, expression, and purification of LASV Z The Z gene of Lassa strain AV was cloned into a pOPINF or pOPINJ vector ( ) using the In-Fusion HD EcoDry Cloning kit. .. The Z protein with N-terminal His6 fusion tag (pOPINF) or N-terminal GST fusion protein (pOPINJ) was expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17 °C overnight using terrific broth medium (supplemented with 100 μm ZnSO4 ) and 0.5 mm isopropyl β-d -thiogalactopyranoside for induction.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan). .. Fifty microgram plasmid of identified positive clone was transiently transfected into 1×106 purified T. gondii according to the description elsewhere .

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: The cDNAs encoding the full-length human α4, β7, and mouse β7 proteins were amplified by RT-PCR and cloned into pCDNA3.1 or pLVX lentiviral expression vector. .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Nested PCR:

    Article Title: Potency and Resistance Analysis of Hepatitis C Virus NS5B Polymerase Inhibitor BMS-791325 on All Major Genotypes
    Article Snippet: Patient-derived NS5Bs were amplified by nested PCR with Platinum Taq high-fidelity DNA polymerase (Invitrogen). .. Amplicons were subjected directly to sequence analysis and cloned into the GT1a shuttle replicon for GT3a and the GT1b shuttle replicon for GT2b to -6 using the in-fusion HD EcoDry cloning kit (Clontech, Mountain View, CA, USA).

    Plasmid Preparation:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech). .. Additional tau 3’-UTR sequences were subcloned from the wild-type human tau 3’-UTR vector using the same method.

    Article Title: Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping
    Article Snippet: .. Production of bacterial artificial chromosomes (BACs) The cDNA amplicons were inserted into the BAC vector pBeloBAC11 (New England BioLabs) using the In-Fusion HD Eco-Dry Cloning Kit (Clontech). .. Briefly, pBeloBAC11 was converted to a linearized form using the long PCR as above, with primers Kos15_NotI_pBelF and Kos15_NotI_pBelR (Additional file ), which contain 15-nt overhangs identical to regions of the primers used for the preparation of the cDNA amplicons.

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: The cDNAs encoding the full-length human α4, β7, and mouse β7 proteins were amplified by RT-PCR and cloned into pCDNA3.1 or pLVX lentiviral expression vector. .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Article Title: Fibroblast activation protein (FAP) as a novel metabolic target
    Article Snippet: .. Amplified LIC-Sumo vector and FGF21 gene fragment were mixed together, treated using In-Fusion HD EcoDry Cloning Plus systems kit and transfected into stellar E. coli competent cells (both Clontech). .. Positive clones were selected on ampicillin/LB agar plate with single colonies picked to amplify plasmids with QIA prep miniprep kit (Qiagen).

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: .. We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Article Title: Biochemical characterization of the Lassa virus L protein
    Article Snippet: .. Cloning, expression, and purification of LASV Z The Z gene of Lassa strain AV was cloned into a pOPINF or pOPINJ vector ( ) using the In-Fusion HD EcoDry Cloning kit. .. The Z protein with N-terminal His6 fusion tag (pOPINF) or N-terminal GST fusion protein (pOPINJ) was expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17 °C overnight using terrific broth medium (supplemented with 100 μm ZnSO4 ) and 0.5 mm isopropyl β-d -thiogalactopyranoside for induction.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. The CT-myc and HA-CT constructs were used as parent plasmids to individually mutate the cysteines at positions 269, 271, and 357 to either serine or alanine.

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: EGFR IIIV/KKRE was amplified by PCR using primers containing infusion tags (described in Supplementary Table ) for the insertion of the PCR product into the pOPINE vector cut with Nco I and Pme I (gift from Prof Ray Owens, University of Oxford). .. The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan). .. Fifty microgram plasmid of identified positive clone was transiently transfected into 1×106 purified T. gondii according to the description elsewhere .

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. The sUL16-HA, sNT-HA, sNT-GFP, and sCT-HA constructs were cloned into the pEGFP-N2 vector by use of an In-Fusion EcoDry cloning kit (Clontech). ..

    Positron Emission Tomography:

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: A modified pET-15b vector encoding a His6-AviTag at the N terminus of the integrin cytoplasmic tail was used as a recombinant model protein affinity matrix as previously described ( ). cDNA encoding integrin β7 (730–779) cytoplasmic tails, WT, β7(L758A), and β7(Y759A) were cloned into this vector, replacing the αIIb tail sequence, and recombinant model proteins were produced as described previously ( ). .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Produced:

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: A modified pET-15b vector encoding a His6-AviTag at the N terminus of the integrin cytoplasmic tail was used as a recombinant model protein affinity matrix as previously described ( ). cDNA encoding integrin β7 (730–779) cytoplasmic tails, WT, β7(L758A), and β7(Y759A) were cloned into this vector, replacing the αIIb tail sequence, and recombinant model proteins were produced as described previously ( ). .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.).

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: The L834R-EGFR pCDNA3 and T766M-EGFR pCDNA3 plasmids were produced through site-directed mutagenesis using the primers described in Supplementary Table . .. The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions.

    FLAG-tag:

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: As an alternative strategy, we introduced a FLAG tag to the C-terminus of P104 and transfected into RH parasites using pMini.3×Flag.ht. vector under the control of GRA1 promoter . .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan).

    BAC Assay:

    Article Title: Strategy for efficient generation of numerous full-length cDNA clones of classical swine fever virus for haplotyping
    Article Snippet: .. Production of bacterial artificial chromosomes (BACs) The cDNA amplicons were inserted into the BAC vector pBeloBAC11 (New England BioLabs) using the In-Fusion HD Eco-Dry Cloning Kit (Clontech). .. Briefly, pBeloBAC11 was converted to a linearized form using the long PCR as above, with primers Kos15_NotI_pBelF and Kos15_NotI_pBelR (Additional file ), which contain 15-nt overhangs identical to regions of the primers used for the preparation of the cDNA amplicons.

    Staining:

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: Intracellular T. gondii was prepared by the infection of Vero cells cultured in an eight-well chamber slide for 48 h; the slide was fixed and stained as described above. .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan).

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    TaKaRa in fusion hd ecodry cloning kit
    In Fusion Hd Ecodry Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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