in fusion ecodry hd cloning kit  (TaKaRa)

 
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  • 95
    Name:
    In Fusion HD EcoDry Cloning Kit
    Description:
    In Fusion Cloning offers a one step high efficiency and high throughput cloning method for projects that require a large number of cloning reactions such as expression screening studies and library construction
    Catalog Number:
    639691
    Price:
    None
    Size:
    96 Rxns
    Category:
    In Fusion Cloning for high throughput workflows In Fusion Cloning Cloning
    Buy from Supplier


    Structured Review

    TaKaRa in fusion ecodry hd cloning kit
    In Fusion Cloning offers a one step high efficiency and high throughput cloning method for projects that require a large number of cloning reactions such as expression screening studies and library construction
    https://www.bioz.com/result/in fusion ecodry hd cloning kit/product/TaKaRa
    Average 95 stars, based on 4 article reviews
    Price from $9.99 to $1999.99
    in fusion ecodry hd cloning kit - by Bioz Stars, 2020-07
    95/100 stars

    Images

    Related Articles

    Clone Assay:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech). .. Additional tau 3’-UTR sequences were subcloned from the wild-type human tau 3’-UTR vector using the same method.

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: .. The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.). .. The cDNAs encoding the ectodomain of human and mouse MAdCAM-1 proteins were amplified by RT-PCR and fused to a cDNA encoding an IgG Fc fragment into pCDNA3.1.

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: .. We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Article Title: Biochemical characterization of the Lassa virus L protein
    Article Snippet: .. Cloning, expression, and purification of LASV Z The Z gene of Lassa strain AV was cloned into a pOPINF or pOPINJ vector ( ) using the In-Fusion HD EcoDry Cloning kit. .. The Z protein with N-terminal His6 fusion tag (pOPINF) or N-terminal GST fusion protein (pOPINJ) was expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17 °C overnight using terrific broth medium (supplemented with 100 μm ZnSO4 ) and 0.5 mm isopropyl β-d -thiogalactopyranoside for induction.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. The CT-myc and HA-CT constructs were used as parent plasmids to individually mutate the cysteines at positions 269, 271, and 357 to either serine or alanine.

    Article Title: The architecture of EGFR’s basal complexes reveals autoinhibition mechanisms in dimers and oligomers
    Article Snippet: .. The In-Fusion® HD EcoDry™ Cloning Plus kit (Takara) was used according to the manufacturer’s instructions. .. Transfections were performed in solution at the same time as seeding, using Viafect (Promega) as a carrier for the I942E-EGFR, L834R-EGFR, T766M-EGFR and wtEGFR plasmids.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan). .. Fifty microgram plasmid of identified positive clone was transiently transfected into 1×106 purified T. gondii according to the description elsewhere .

    Amplification:

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: .. The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Mutagenesis:

    Article Title: Transmission of integrin β7 transmembrane domain topology enables gut lymphoid tissue development
    Article Snippet: .. Cloning of β7 point mutants (L758A, Y759A, L721P, G722P, and L723P) was done by oligonucleotide-directed mutagenesis using the Infusion-HD Eco Dry cloning kit (Takara Bio Inc.). .. The cDNAs encoding the ectodomain of human and mouse MAdCAM-1 proteins were amplified by RT-PCR and fused to a cDNA encoding an IgG Fc fragment into pCDNA3.1.

    Construct:

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: .. We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Purification:

    Article Title: Biochemical characterization of the Lassa virus L protein
    Article Snippet: .. Cloning, expression, and purification of LASV Z The Z gene of Lassa strain AV was cloned into a pOPINF or pOPINJ vector ( ) using the In-Fusion HD EcoDry Cloning kit. .. The Z protein with N-terminal His6 fusion tag (pOPINF) or N-terminal GST fusion protein (pOPINJ) was expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17 °C overnight using terrific broth medium (supplemented with 100 μm ZnSO4 ) and 0.5 mm isopropyl β-d -thiogalactopyranoside for induction.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan). .. Fifty microgram plasmid of identified positive clone was transiently transfected into 1×106 purified T. gondii according to the description elsewhere .

    Sequencing:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech). .. Additional tau 3’-UTR sequences were subcloned from the wild-type human tau 3’-UTR vector using the same method.

    Generated:

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. The CT-myc and HA-CT constructs were used as parent plasmids to individually mutate the cysteines at positions 269, 271, and 357 to either serine or alanine.

    Expressing:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech). .. Additional tau 3’-UTR sequences were subcloned from the wild-type human tau 3’-UTR vector using the same method.

    Article Title: Biochemical characterization of the Lassa virus L protein
    Article Snippet: .. Cloning, expression, and purification of LASV Z The Z gene of Lassa strain AV was cloned into a pOPINF or pOPINJ vector ( ) using the In-Fusion HD EcoDry Cloning kit. .. The Z protein with N-terminal His6 fusion tag (pOPINF) or N-terminal GST fusion protein (pOPINJ) was expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17 °C overnight using terrific broth medium (supplemented with 100 μm ZnSO4 ) and 0.5 mm isopropyl β-d -thiogalactopyranoside for induction.

    Polymerase Chain Reaction:

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan). .. Fifty microgram plasmid of identified positive clone was transiently transfected into 1×106 purified T. gondii according to the description elsewhere .

    Transformation Assay:

    Article Title: Streptococcus oralis Neuraminidase Modulates Adherence to Multiple Carbohydrates on Platelets
    Article Snippet: .. The spectinomycin resistance cassette, amplified by using primers S.F and S.R, and flanking fragments were cloned into EcoRI-digested pDrive (Qiagen, Germantown, MD) by use of an In-Fusion EcoDry HD cloning kit (Clontech, Mountain View, CA) per the manufacturer's protocol and then transformed into E. coli Stellar (Clontech). .. Plasmid constructs were confirmed by PCR with the M13F and T7 promoter primers followed by sequencing.

    Plasmid Preparation:

    Article Title: Alternative Polyadenylation and miR-34 Family Members Regulate Tau Expression †
    Article Snippet: .. This sequence was inserted into the pmirGLO Dual-Luciferase miRNA Target Expression Vector (Promega) between the XhoI and SalI restriction sites using the In-Fusion HD EcoDry Cloning Kit (Clontech). .. Additional tau 3’-UTR sequences were subcloned from the wild-type human tau 3’-UTR vector using the same method.

    Article Title: Peptide Uptake Is Essential for Borrelia burgdorferi Viability and Involves Structural and Regulatory Complexity of its Oligopeptide Transporter
    Article Snippet: .. We first constructed IPTG-inducible bb0334–35 (oppDF ) in E. coli-B. burgdorferi shuttle vector pJSB275 containing PflgB-aadA (Strepr ) ( ) using an InFusion HD EcoDry cloning kit (Clontech, Mountain View, CA). .. The bb0334–35 insertion was generated by amplification with CloneAmp hi-fi PCR Premix (Clontech, Mountain View, CA) using B31 5A18 NP1 genomic DNA as a template and primers 5′ bb0334-35ind and 3′ bb0334-35ind.

    Article Title: Biochemical characterization of the Lassa virus L protein
    Article Snippet: .. Cloning, expression, and purification of LASV Z The Z gene of Lassa strain AV was cloned into a pOPINF or pOPINJ vector ( ) using the In-Fusion HD EcoDry Cloning kit. .. The Z protein with N-terminal His6 fusion tag (pOPINF) or N-terminal GST fusion protein (pOPINJ) was expressed in E. coli strain BL21 Gold (DE3) (Novagen) at 17 °C overnight using terrific broth medium (supplemented with 100 μm ZnSO4 ) and 0.5 mm isopropyl β-d -thiogalactopyranoside for induction.

    Article Title: Domain Interaction Studies of Herpes Simplex Virus 1 Tegument Protein UL16 Reveal Its Interaction with Mitochondria
    Article Snippet: .. CT-myc, NT-HA, and HA-CT were generated in the pEGFP-N2 vector by use of an In-Fusion Ecodry cloning kit (Clontech). .. The CT-myc and HA-CT constructs were used as parent plasmids to individually mutate the cysteines at positions 269, 271, and 357 to either serine or alanine.

    Article Title: A Novel PAN/Apple Domain-Containing Protein from Toxoplasma gondii: Characterization and Receptor Identification
    Article Snippet: .. The purified PCR product was blunted with DNA blunting kit (TaKaRa, Japan) and then inserted into digested and blunted pMini.3×Flag.ht. vector using In-Fusion HD EcoDry cloning kit (Clontech, Japan). .. Fifty microgram plasmid of identified positive clone was transiently transfected into 1×106 purified T. gondii according to the description elsewhere .

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  • 94
    TaKaRa dsred vegt pbe ds3 utr
    Nanos1 represses <t>VegT</t> translation in PGCs. ( A ) RNA EMSA analysis shows that the Xenopus Pumilio1 RNA-binding domain (Xpum1 RBD) can bind biotin-labeled VegT <t>PBE</t> (1 ng) but not the mutant VegT PBE. This binding reaction was competed by unlabeled VegT PBE. ( B ) Experimental design for results shown in C. Venus-DEADSouth <t>3′UTR</t> and <t>DsRED-DEADSouth</t> 3′UTR are germline lineage tracers. VegT PBE or its mutant was subcloned downstream of the DsRED open reading frame. Nanos1-MO was injected at stage 1. Germline lineage reporters were injected into four germ plasm-containing blastomeres at the 32-cell stage. Embryos were allowed to develop until stage 35 and the fluorescent images were taken from live embryos. ( C ) In vivo fluorescent reporter assay indicates that VegT PBE mediates translational repression of DsRED (red) in PGCs (green). Repression was lost in embryos bearing a PBE mutation or that were depleted of Nanos1. Reporters injected are noted across the top. This experiment was repeated three times. Arrows indicate DsRED signal detected in PGCs. Data from one experiment are presented beneath the images for each group. Scale bars: 50 μm.
    Dsred Vegt Pbe Ds3 Utr, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/dsred vegt pbe ds3 utr/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    dsred vegt pbe ds3 utr - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    89
    TaKaRa in fusion hd ecodry cloning kit
    Nanos1 represses <t>VegT</t> translation in PGCs. ( A ) RNA EMSA analysis shows that the Xenopus Pumilio1 RNA-binding domain (Xpum1 RBD) can bind biotin-labeled VegT <t>PBE</t> (1 ng) but not the mutant VegT PBE. This binding reaction was competed by unlabeled VegT PBE. ( B ) Experimental design for results shown in C. Venus-DEADSouth <t>3′UTR</t> and <t>DsRED-DEADSouth</t> 3′UTR are germline lineage tracers. VegT PBE or its mutant was subcloned downstream of the DsRED open reading frame. Nanos1-MO was injected at stage 1. Germline lineage reporters were injected into four germ plasm-containing blastomeres at the 32-cell stage. Embryos were allowed to develop until stage 35 and the fluorescent images were taken from live embryos. ( C ) In vivo fluorescent reporter assay indicates that VegT PBE mediates translational repression of DsRED (red) in PGCs (green). Repression was lost in embryos bearing a PBE mutation or that were depleted of Nanos1. Reporters injected are noted across the top. This experiment was repeated three times. Arrows indicate DsRED signal detected in PGCs. Data from one experiment are presented beneath the images for each group. Scale bars: 50 μm.
    In Fusion Hd Ecodry Cloning Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 89/100, based on 36 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in fusion hd ecodry cloning kit/product/TaKaRa
    Average 89 stars, based on 36 article reviews
    Price from $9.99 to $1999.99
    in fusion hd ecodry cloning kit - by Bioz Stars, 2020-07
    89/100 stars
      Buy from Supplier

    94
    TaKaRa in fusion hd ecodry cloning plus systems kit
    Nanos1 represses <t>VegT</t> translation in PGCs. ( A ) RNA EMSA analysis shows that the Xenopus Pumilio1 RNA-binding domain (Xpum1 RBD) can bind biotin-labeled VegT <t>PBE</t> (1 ng) but not the mutant VegT PBE. This binding reaction was competed by unlabeled VegT PBE. ( B ) Experimental design for results shown in C. Venus-DEADSouth <t>3′UTR</t> and <t>DsRED-DEADSouth</t> 3′UTR are germline lineage tracers. VegT PBE or its mutant was subcloned downstream of the DsRED open reading frame. Nanos1-MO was injected at stage 1. Germline lineage reporters were injected into four germ plasm-containing blastomeres at the 32-cell stage. Embryos were allowed to develop until stage 35 and the fluorescent images were taken from live embryos. ( C ) In vivo fluorescent reporter assay indicates that VegT PBE mediates translational repression of DsRED (red) in PGCs (green). Repression was lost in embryos bearing a PBE mutation or that were depleted of Nanos1. Reporters injected are noted across the top. This experiment was repeated three times. Arrows indicate DsRED signal detected in PGCs. Data from one experiment are presented beneath the images for each group. Scale bars: 50 μm.
    In Fusion Hd Ecodry Cloning Plus Systems Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/in fusion hd ecodry cloning plus systems kit/product/TaKaRa
    Average 94 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    in fusion hd ecodry cloning plus systems kit - by Bioz Stars, 2020-07
    94/100 stars
      Buy from Supplier

    Image Search Results


    Nanos1 represses VegT translation in PGCs. ( A ) RNA EMSA analysis shows that the Xenopus Pumilio1 RNA-binding domain (Xpum1 RBD) can bind biotin-labeled VegT PBE (1 ng) but not the mutant VegT PBE. This binding reaction was competed by unlabeled VegT PBE. ( B ) Experimental design for results shown in C. Venus-DEADSouth 3′UTR and DsRED-DEADSouth 3′UTR are germline lineage tracers. VegT PBE or its mutant was subcloned downstream of the DsRED open reading frame. Nanos1-MO was injected at stage 1. Germline lineage reporters were injected into four germ plasm-containing blastomeres at the 32-cell stage. Embryos were allowed to develop until stage 35 and the fluorescent images were taken from live embryos. ( C ) In vivo fluorescent reporter assay indicates that VegT PBE mediates translational repression of DsRED (red) in PGCs (green). Repression was lost in embryos bearing a PBE mutation or that were depleted of Nanos1. Reporters injected are noted across the top. This experiment was repeated three times. Arrows indicate DsRED signal detected in PGCs. Data from one experiment are presented beneath the images for each group. Scale bars: 50 μm.

    Journal: Development (Cambridge, England)

    Article Title: Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells

    doi: 10.1242/dev.079608

    Figure Lengend Snippet: Nanos1 represses VegT translation in PGCs. ( A ) RNA EMSA analysis shows that the Xenopus Pumilio1 RNA-binding domain (Xpum1 RBD) can bind biotin-labeled VegT PBE (1 ng) but not the mutant VegT PBE. This binding reaction was competed by unlabeled VegT PBE. ( B ) Experimental design for results shown in C. Venus-DEADSouth 3′UTR and DsRED-DEADSouth 3′UTR are germline lineage tracers. VegT PBE or its mutant was subcloned downstream of the DsRED open reading frame. Nanos1-MO was injected at stage 1. Germline lineage reporters were injected into four germ plasm-containing blastomeres at the 32-cell stage. Embryos were allowed to develop until stage 35 and the fluorescent images were taken from live embryos. ( C ) In vivo fluorescent reporter assay indicates that VegT PBE mediates translational repression of DsRED (red) in PGCs (green). Repression was lost in embryos bearing a PBE mutation or that were depleted of Nanos1. Reporters injected are noted across the top. This experiment was repeated three times. Arrows indicate DsRED signal detected in PGCs. Data from one experiment are presented beneath the images for each group. Scale bars: 50 μm.

    Article Snippet: VegT 3′UTR (296 nt, 2336-2631) containing the PBE (UGUAAAUA) was subcloned downstream of the DsRED open reading frame to produce DsRED- VegT PBE-DS3′UTR (In-Fusion HD EcoDry Cloning System, Clontech).

    Techniques: RNA Binding Assay, Labeling, Mutagenesis, Binding Assay, Injection, In Vivo, Reporter Assay

    VegT RNA is stabilized in PGCs after Nanos1 knockdown. ( A ) VegT PBE mediates the degradation of the reporter message DsRED. PGCs were detected by either WISH for Xpat (top panels) or for DsRED antisense probe (bottom panels) in tailbud stage 31 embryos. Note that the DsRED reporter with a PBE was specifically degraded. Arrows indicate PGCs. ( B ) Real-time PCR analysis of VegT expression at stages 8 and 10. Expression of VegT in Nanos1 knockdown samples was normalized to that of wild-type samples. Real-time PCR was performed in duplicate, and experiments were repeated three times and showed a similar pattern. Error bars indicate s.e. P -value by unpaired Student’s t -test.

    Journal: Development (Cambridge, England)

    Article Title: Xenopus Nanos1 is required to prevent endoderm gene expression and apoptosis in primordial germ cells

    doi: 10.1242/dev.079608

    Figure Lengend Snippet: VegT RNA is stabilized in PGCs after Nanos1 knockdown. ( A ) VegT PBE mediates the degradation of the reporter message DsRED. PGCs were detected by either WISH for Xpat (top panels) or for DsRED antisense probe (bottom panels) in tailbud stage 31 embryos. Note that the DsRED reporter with a PBE was specifically degraded. Arrows indicate PGCs. ( B ) Real-time PCR analysis of VegT expression at stages 8 and 10. Expression of VegT in Nanos1 knockdown samples was normalized to that of wild-type samples. Real-time PCR was performed in duplicate, and experiments were repeated three times and showed a similar pattern. Error bars indicate s.e. P -value by unpaired Student’s t -test.

    Article Snippet: VegT 3′UTR (296 nt, 2336-2631) containing the PBE (UGUAAAUA) was subcloned downstream of the DsRED open reading frame to produce DsRED- VegT PBE-DS3′UTR (In-Fusion HD EcoDry Cloning System, Clontech).

    Techniques: Real-time Polymerase Chain Reaction, Expressing