immunopure streptavidin  (Thermo Fisher)


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    Structured Review

    Thermo Fisher immunopure streptavidin
    Immunopure Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopure streptavidin/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    immunopure streptavidin - by Bioz Stars, 2020-03
    99/100 stars

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    Related Articles

    Centrifugation:

    Article Title: Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens
    Article Snippet: After centrifugation at 17,000 × g for 5 min, 50 μl of the clarified serum dilution was added to each well of a 96-well filter bottom plate (Millipore, Bedford, MA). .. The development protocol using biotinylated, monoclonal anti-IgG (clone H4; Southern Biotech) and anti-IgG4 (clone HP6025; Zymed) antibodies and R -phycoerythrin-labeled streptavidin (Invitrogen) has been previously described ( ).

    Amplification:

    Article Title: Rapid Microsphere Assay for Identification of Cryptosporidium hominis and Cryptosporidium parvum in Stool and Environmental Samples ▿
    Article Snippet: PCR product was added, the titer plate was sealed, and the amplified DNA was denatured at 95°C for 5 min followed by incubation for 50 min at 42°C in a thermocycler (PTC 200; MJ Research, Bio-Rad, Hercules, CA). .. After incubation, the plate was centrifuged at 3,580 × g for 5 min, 25 μl of supernatant was carefully removed, and 75 μl of a 1:40 dilution of streptavidin-R-phycoerythrin (SA-PE) (Molecular Probes, Eugene, OR) was added.

    Cytometry:

    Article Title: The anti-apoptotic activities of Rel and RelA required during B-cell maturation involve the regulation of Bcl-2 expression
    Article Snippet: Staining with biotinylated antibodies was revealed by R-phycoerythrin–streptavidin or tricolor–streptavidin (Caltag, San Francisco, CA). .. Between 5000 and 10 000 viable cells were analyzed using a FACScan flow cytometer (Becton Dickinson, San Jose, CA).

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: Paragraph title: Immunofluorescence Staining and Flow Cytometry. ... Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent.

    Article Title: T cell-specific FADD-deficient mice: FADD is required for early T cell development
    Article Snippet: Paragraph title: Flow Cytometry. ... CD4, CD8, B220, and streptavidin-tricolor antibodies were purchased from Caltag.

    Construct:

    Article Title: Nanometer-accuracy distance measurements between fluorophores at the single-molecule level
    Article Snippet: .. Samples for imaging are prepared by adding 10 µL of 5 mg/mL Biotin-BSA (29130; Thermo Fisher Scientific) in BRB80 to the flow cell, incubating for 2 min, adding 10 µL of 5 mg/mL Biotin-BSA in BRB80, incubating for 2 min, washing with 20 µL of BRB80, adding 10 µL of 0.5 mg/mL streptavidin (S888; Thermo Fisher Scientific) in PBS pH 7.4, and incubating for 2 min. We then washed with 20 µL of PBS (pH 7.4), added 10 µL of 3 pM dsDNA construct in PBS (pH 7.4), and incubated for 5 min. Next, we washed with 30 µL of PBS (pH 7.4) and finally added the PCA/PCD/Trolox oxygen scavenging system ( ) in PBS (pH 7.4). ..

    Adsorption:

    Article Title: Threading Immobilized DNA Molecules through a Solid-State Nanopore at > 100 ?s per Base Rate
    Article Snippet: The Cr/Au coated optical fiber tip was immersed in a 10 mg/ml streptavidin (Invitrogen, 434301) solution for 10 min. .. Fluorescently labeled streptavidin was used to verify the adsorption.

    Incubation:

    Article Title: DNA Suspension Arrays: Silencing Discrete Artifacts for High-Sensitivity Applications
    Article Snippet: .. Bead and Streptavidin-R-Phycoerythrin Hybridization, Flow Fluorimetry Approximately 750 of each xTAG bead, competimer and/or DNO (where indicated) were added to each LDR in 1X TMAC buffer (2.5 M tetramethylammonium chloride (TMAC), 0.1 M Tris-HCl (pH 8.0), 3 mM ethylenediaminetetraacetic acid and 0.1% sodium dodecyl sulfate) in 60 µl and subjected to 95°C (5 min.) and 37°C (45 min.), followed by addition of a Streptavidin-R-phycoerythrin conjugate (100 ng/well; Invitrogen #S-866) in 6 µl 1X TMAC buffer and incubation at 37°C (45 min.). .. Seventy-five beads per region were measured on a Luminex 200 instrument with BioPlex Manager software (Bio-Rad Life Sciences, Hercules, CA) at the highest reporter channel photomultiplier tube voltage setting (typically 700–750 V).

    Article Title: Nanometer-accuracy distance measurements between fluorophores at the single-molecule level
    Article Snippet: .. Samples for imaging are prepared by adding 10 µL of 5 mg/mL Biotin-BSA (29130; Thermo Fisher Scientific) in BRB80 to the flow cell, incubating for 2 min, adding 10 µL of 5 mg/mL Biotin-BSA in BRB80, incubating for 2 min, washing with 20 µL of BRB80, adding 10 µL of 0.5 mg/mL streptavidin (S888; Thermo Fisher Scientific) in PBS pH 7.4, and incubating for 2 min. We then washed with 20 µL of PBS (pH 7.4), added 10 µL of 3 pM dsDNA construct in PBS (pH 7.4), and incubated for 5 min. Next, we washed with 30 µL of PBS (pH 7.4) and finally added the PCA/PCD/Trolox oxygen scavenging system ( ) in PBS (pH 7.4). ..

    Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression
    Article Snippet: Immunohistochemistry Automated immunohistochemistry (Lab Vision Autostainer LV-1; Lab Vision Corporation, Fremont, CA, USA) was performed with the streptavidin–biotin–peroxidase technique, using antibodies raised against human PDGFR-α (1:200 dilution; Lab Vision Corporation) and PDGF-A (clone N-30; 1:80 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. Endogenous peroxidase activity was blocked by the incubation of slides in 3% hydrogen peroxide in methanol for 10 min, and non-specific epitopes were eliminated by incubation with a blocking solution (UltraVision Block; Lab Vision Corporation) for 10 min.

    Article Title: T cell-specific FADD-deficient mice: FADD is required for early T cell development
    Article Snippet: One million peripheral lymphoid cells were incubated with anti-FcγII/III receptor antibody (2.4G2, PharMingen) and normal rabbit serum for 10 min 4°C before cell surface staining. .. CD4, CD8, B220, and streptavidin-tricolor antibodies were purchased from Caltag.

    Article Title: Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens
    Article Snippet: A 1:400 dilution of serum in PBS containing 0.5% BSA, 0.05% Tween 20, 0.02% sodium azide, 0.5% poly(vinyl alcohol), and 0.8% polyvinylpyrrolidone was incubated for 1 h at 37°C and stored overnight at 4°C. .. The development protocol using biotinylated, monoclonal anti-IgG (clone H4; Southern Biotech) and anti-IgG4 (clone HP6025; Zymed) antibodies and R -phycoerythrin-labeled streptavidin (Invitrogen) has been previously described ( ).

    Article Title: Mechanical unfolding of human telomere G-quadruplex DNA probed by integrated fluorescence and magnetic tweezers spectroscopy
    Article Snippet: .. Channels (∼10 µL total volume) were treated with 35 µL of 1 mg/mL of biotinylated bovine serum albumin (Sigma Cat. No.A8549) for 5 min, washed with 100 µL of T50 buffer [10 mM Tris–HCl (pH 8), 50 mM NaCl], incubated with 50 µL of 0.2 mg/mL streptavidin (Invitrogen Cat. #S888) and then washed with 100 µL of T50. ..

    Article Title: Rapid Microsphere Assay for Identification of Cryptosporidium hominis and Cryptosporidium parvum in Stool and Environmental Samples ▿
    Article Snippet: .. After incubation, the plate was centrifuged at 3,580 × g for 5 min, 25 μl of supernatant was carefully removed, and 75 μl of a 1:40 dilution of streptavidin-R-phycoerythrin (SA-PE) (Molecular Probes, Eugene, OR) was added. ..

    Activity Assay:

    Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression
    Article Snippet: Immunohistochemistry Automated immunohistochemistry (Lab Vision Autostainer LV-1; Lab Vision Corporation, Fremont, CA, USA) was performed with the streptavidin–biotin–peroxidase technique, using antibodies raised against human PDGFR-α (1:200 dilution; Lab Vision Corporation) and PDGF-A (clone N-30; 1:80 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. Endogenous peroxidase activity was blocked by the incubation of slides in 3% hydrogen peroxide in methanol for 10 min, and non-specific epitopes were eliminated by incubation with a blocking solution (UltraVision Block; Lab Vision Corporation) for 10 min.

    Article Title: Active nematic emulsions
    Article Snippet: MTs were mixed with the molecular motor clusters that act as active cross-linkers and with ATP (A2383, Sigma) that drives the activity of the gel. .. To maintain a constant concentration of ATP during the experiments, we added an enzymatic ATP-regenerator system based on phosphoenolpyruvate (P7127, Sigma) and pyruvate kinase (434301, Invitrogen), which converted adenosine 5′-diphosphate back into ATP.

    Expressing:

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: The expression of class II MHC, B7.2, and CD25 was analyzed on resting and anti-IgM– or anti-CD40–treated splenic B cells were stimulated for 24 h. Cells were surface stained with biotinylated monoclonal antibodies M5/114 (anti-class II MHC), GL-1 (anti-B7.2), PC16 (anti–IL-2 receptor α chain CD25), or isotype-matched control monoclonal antibodies as described previously ( ). .. Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent.

    Hybridization:

    Article Title: DNA Suspension Arrays: Silencing Discrete Artifacts for High-Sensitivity Applications
    Article Snippet: .. Bead and Streptavidin-R-Phycoerythrin Hybridization, Flow Fluorimetry Approximately 750 of each xTAG bead, competimer and/or DNO (where indicated) were added to each LDR in 1X TMAC buffer (2.5 M tetramethylammonium chloride (TMAC), 0.1 M Tris-HCl (pH 8.0), 3 mM ethylenediaminetetraacetic acid and 0.1% sodium dodecyl sulfate) in 60 µl and subjected to 95°C (5 min.) and 37°C (45 min.), followed by addition of a Streptavidin-R-phycoerythrin conjugate (100 ng/well; Invitrogen #S-866) in 6 µl 1X TMAC buffer and incubation at 37°C (45 min.). .. Seventy-five beads per region were measured on a Luminex 200 instrument with BioPlex Manager software (Bio-Rad Life Sciences, Hercules, CA) at the highest reporter channel photomultiplier tube voltage setting (typically 700–750 V).

    Article Title: Rapid Microsphere Assay for Identification of Cryptosporidium hominis and Cryptosporidium parvum in Stool and Environmental Samples ▿
    Article Snippet: Paragraph title: Hybridization assay procedure. ... After incubation, the plate was centrifuged at 3,580 × g for 5 min, 25 μl of supernatant was carefully removed, and 75 μl of a 1:40 dilution of streptavidin-R-phycoerythrin (SA-PE) (Molecular Probes, Eugene, OR) was added.

    Immunohistochemistry:

    Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression
    Article Snippet: .. Immunohistochemistry Automated immunohistochemistry (Lab Vision Autostainer LV-1; Lab Vision Corporation, Fremont, CA, USA) was performed with the streptavidin–biotin–peroxidase technique, using antibodies raised against human PDGFR-α (1:200 dilution; Lab Vision Corporation) and PDGF-A (clone N-30; 1:80 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA). ..

    Concentration Assay:

    Article Title: A trap-and-release integrated microfluidic system for dynamic microarray applications
    Article Snippet: .. The streptavidin solution was prepared by dissolving Streptavidin Alexa Fluor 546 conjugate (Invitrogen, Carlsbad, CA) in PBS to obtain a concentration of 0.05 mg/ml PBS. .. Micro manipulation system (Sigma Koki, Saitama, Japan) was used to create microbubbles in our experiments.

    Article Title: Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens
    Article Snippet: In order to reduce nonspecific binding, extract from an isopropyl-β- d -thiogalactopyranoside (IPTG)-induced culture of pGEX4T-2 in BL21 E. coli cells was included in each serum diluent at a final concentration of 3 μg/ml ( ). .. The development protocol using biotinylated, monoclonal anti-IgG (clone H4; Southern Biotech) and anti-IgG4 (clone HP6025; Zymed) antibodies and R -phycoerythrin-labeled streptavidin (Invitrogen) has been previously described ( ).

    Article Title: Active nematic emulsions
    Article Snippet: .. To maintain a constant concentration of ATP during the experiments, we added an enzymatic ATP-regenerator system based on phosphoenolpyruvate (P7127, Sigma) and pyruvate kinase (434301, Invitrogen), which converted adenosine 5′-diphosphate back into ATP. ..

    Article Title: Rapid Microsphere Assay for Identification of Cryptosporidium hominis and Cryptosporidium parvum in Stool and Environmental Samples ▿
    Article Snippet: To prepare the microsphere mixture, a calculated volume of each microsphere set was added to 1.5× TMAC buffer (1.5× TMAC buffer is 4.5 M tetramethylammonium chloride, 75 mM Tris-HCl, pH 8.0, 6 mM EDTA, and 0.15% Sarkosyl) to achieve a concentration of 1,500 to 2,500 microspheres per set in 33 μl. .. After incubation, the plate was centrifuged at 3,580 × g for 5 min, 25 μl of supernatant was carefully removed, and 75 μl of a 1:40 dilution of streptavidin-R-phycoerythrin (SA-PE) (Molecular Probes, Eugene, OR) was added.

    other:

    Article Title: Src1 is a Protein of the Inner Nuclear Membrane Interacting with the Dictyostelium Lamin NE81
    Article Snippet: Antibodies and Streptavidin Conjugates Primary antibodies: rat anti-Src1 (this work), rabbit anti-NE81 [ ], rat YL1/2 [ ], rabbit anti-GFP [ ], rabbit anti-GFP (Molecular Probes, A-6455; Life Technologies, Carlsbad, CA, USA).

    Imaging:

    Article Title: Nanometer-accuracy distance measurements between fluorophores at the single-molecule level
    Article Snippet: .. Samples for imaging are prepared by adding 10 µL of 5 mg/mL Biotin-BSA (29130; Thermo Fisher Scientific) in BRB80 to the flow cell, incubating for 2 min, adding 10 µL of 5 mg/mL Biotin-BSA in BRB80, incubating for 2 min, washing with 20 µL of BRB80, adding 10 µL of 0.5 mg/mL streptavidin (S888; Thermo Fisher Scientific) in PBS pH 7.4, and incubating for 2 min. We then washed with 20 µL of PBS (pH 7.4), added 10 µL of 3 pM dsDNA construct in PBS (pH 7.4), and incubated for 5 min. Next, we washed with 30 µL of PBS (pH 7.4) and finally added the PCA/PCD/Trolox oxygen scavenging system ( ) in PBS (pH 7.4). ..

    Polymerase Chain Reaction:

    Article Title: Rapid Microsphere Assay for Identification of Cryptosporidium hominis and Cryptosporidium parvum in Stool and Environmental Samples ▿
    Article Snippet: PCR product was added, the titer plate was sealed, and the amplified DNA was denatured at 95°C for 5 min followed by incubation for 50 min at 42°C in a thermocycler (PTC 200; MJ Research, Bio-Rad, Hercules, CA). .. After incubation, the plate was centrifuged at 3,580 × g for 5 min, 25 μl of supernatant was carefully removed, and 75 μl of a 1:40 dilution of streptavidin-R-phycoerythrin (SA-PE) (Molecular Probes, Eugene, OR) was added.

    Binding Assay:

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: .. Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent. .. Viable (PI negative) cells (5,000–10,000) were analyzed in a FACScan® flow cytometer ( Becton Dickinson ).

    Article Title: Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens
    Article Snippet: In order to reduce nonspecific binding, extract from an isopropyl-β- d -thiogalactopyranoside (IPTG)-induced culture of pGEX4T-2 in BL21 E. coli cells was included in each serum diluent at a final concentration of 3 μg/ml ( ). .. The development protocol using biotinylated, monoclonal anti-IgG (clone H4; Southern Biotech) and anti-IgG4 (clone HP6025; Zymed) antibodies and R -phycoerythrin-labeled streptavidin (Invitrogen) has been previously described ( ).

    Immunofluorescence:

    Article Title: The anti-apoptotic activities of Rel and RelA required during B-cell maturation involve the regulation of Bcl-2 expression
    Article Snippet: Paragraph title: Immunofluorescence staining and flow cytometric analysis ... Staining with biotinylated antibodies was revealed by R-phycoerythrin–streptavidin or tricolor–streptavidin (Caltag, San Francisco, CA).

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: Paragraph title: Immunofluorescence Staining and Flow Cytometry. ... Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent.

    Nucleic Acid Electrophoresis:

    Article Title: Nitroglycerin Tolerance in Caveolin-1 Deficient Mice
    Article Snippet: Samples were immunoprecipitated using streptavidin conjugated antibodies (Dynabeads M-280 Streptavidin from Invitrogen). .. Biotinylated Cav-1 was resolved by non-reducing SDS-polyacrylamide gel electrophoresis (PAGE), transferred and detected by immunoblotting with mouse anti-Cav-1 (1∶1000).

    Fluorescence:

    Article Title: Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens
    Article Snippet: The development protocol using biotinylated, monoclonal anti-IgG (clone H4; Southern Biotech) and anti-IgG4 (clone HP6025; Zymed) antibodies and R -phycoerythrin-labeled streptavidin (Invitrogen) has been previously described ( ). .. Gated data were acquired on at least 100 monodispersed beads of each spectral classification, and the median fluorescence intensity for each analyte was calculated.

    Flow Cytometry:

    Article Title: The anti-apoptotic activities of Rel and RelA required during B-cell maturation involve the regulation of Bcl-2 expression
    Article Snippet: Paragraph title: Immunofluorescence staining and flow cytometric analysis ... Staining with biotinylated antibodies was revealed by R-phycoerythrin–streptavidin or tricolor–streptavidin (Caltag, San Francisco, CA).

    Article Title: DNA Suspension Arrays: Silencing Discrete Artifacts for High-Sensitivity Applications
    Article Snippet: .. Bead and Streptavidin-R-Phycoerythrin Hybridization, Flow Fluorimetry Approximately 750 of each xTAG bead, competimer and/or DNO (where indicated) were added to each LDR in 1X TMAC buffer (2.5 M tetramethylammonium chloride (TMAC), 0.1 M Tris-HCl (pH 8.0), 3 mM ethylenediaminetetraacetic acid and 0.1% sodium dodecyl sulfate) in 60 µl and subjected to 95°C (5 min.) and 37°C (45 min.), followed by addition of a Streptavidin-R-phycoerythrin conjugate (100 ng/well; Invitrogen #S-866) in 6 µl 1X TMAC buffer and incubation at 37°C (45 min.). .. Seventy-five beads per region were measured on a Luminex 200 instrument with BioPlex Manager software (Bio-Rad Life Sciences, Hercules, CA) at the highest reporter channel photomultiplier tube voltage setting (typically 700–750 V).

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: Paragraph title: Immunofluorescence Staining and Flow Cytometry. ... Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent.

    Article Title: Nanometer-accuracy distance measurements between fluorophores at the single-molecule level
    Article Snippet: .. Samples for imaging are prepared by adding 10 µL of 5 mg/mL Biotin-BSA (29130; Thermo Fisher Scientific) in BRB80 to the flow cell, incubating for 2 min, adding 10 µL of 5 mg/mL Biotin-BSA in BRB80, incubating for 2 min, washing with 20 µL of BRB80, adding 10 µL of 0.5 mg/mL streptavidin (S888; Thermo Fisher Scientific) in PBS pH 7.4, and incubating for 2 min. We then washed with 20 µL of PBS (pH 7.4), added 10 µL of 3 pM dsDNA construct in PBS (pH 7.4), and incubated for 5 min. Next, we washed with 30 µL of PBS (pH 7.4) and finally added the PCA/PCD/Trolox oxygen scavenging system ( ) in PBS (pH 7.4). ..

    Article Title: T cell-specific FADD-deficient mice: FADD is required for early T cell development
    Article Snippet: Paragraph title: Flow Cytometry. ... CD4, CD8, B220, and streptavidin-tricolor antibodies were purchased from Caltag.

    Labeling:

    Article Title: Threading Immobilized DNA Molecules through a Solid-State Nanopore at > 100 ?s per Base Rate
    Article Snippet: The Cr/Au coated optical fiber tip was immersed in a 10 mg/ml streptavidin (Invitrogen, 434301) solution for 10 min. .. Fluorescently labeled streptavidin was used to verify the adsorption.

    Mouse Assay:

    Article Title: The anti-apoptotic activities of Rel and RelA required during B-cell maturation involve the regulation of Bcl-2 expression
    Article Snippet: Single-cell suspensions from the bone marrow, spleen, thymus and lymph nodes of reconstituted mice were surface stained as described ( ) using various combinations of monoclonal antibodies conjugated to biotin, fluorescein isothiocyanate (FITC), R-phycoerythrin, Cy5 or Texas Red. .. Staining with biotinylated antibodies was revealed by R-phycoerythrin–streptavidin or tricolor–streptavidin (Caltag, San Francisco, CA).

    Article Title: T cell-specific FADD-deficient mice: FADD is required for early T cell development
    Article Snippet: Cell suspensions were prepared from lymphoid organs of mice 3–8 wk of age. .. CD4, CD8, B220, and streptavidin-tricolor antibodies were purchased from Caltag.

    FACS:

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent. .. For the coculture experiments, c-rel−/− (Ly5.1) cells were separated from control c-rel+/+ (Ly5.2) cells by cell sorting after staining with a biotinylated anti-Ly5.2 monoclonal antibody plus R-PE streptavidin.

    Blocking Assay:

    Article Title: Overexpression of platelet-derived growth factor receptor ? in breast cancer is associated with tumour progression
    Article Snippet: Immunohistochemistry Automated immunohistochemistry (Lab Vision Autostainer LV-1; Lab Vision Corporation, Fremont, CA, USA) was performed with the streptavidin–biotin–peroxidase technique, using antibodies raised against human PDGFR-α (1:200 dilution; Lab Vision Corporation) and PDGF-A (clone N-30; 1:80 dilution; Santa Cruz Biotechnology, Santa Cruz, CA, USA). .. Endogenous peroxidase activity was blocked by the incubation of slides in 3% hydrogen peroxide in methanol for 10 min, and non-specific epitopes were eliminated by incubation with a blocking solution (UltraVision Block; Lab Vision Corporation) for 10 min.

    Article Title: Mechanical unfolding of human telomere G-quadruplex DNA probed by integrated fluorescence and magnetic tweezers spectroscopy
    Article Snippet: Chambers were heated on a hot block (95°C) for 1 min to seal the parafilm to the glass. .. Channels (∼10 µL total volume) were treated with 35 µL of 1 mg/mL of biotinylated bovine serum albumin (Sigma Cat. No.A8549) for 5 min, washed with 100 µL of T50 buffer [10 mM Tris–HCl (pH 8), 50 mM NaCl], incubated with 50 µL of 0.2 mg/mL streptavidin (Invitrogen Cat. #S888) and then washed with 100 µL of T50.

    Polyacrylamide Gel Electrophoresis:

    Article Title: Nitroglycerin Tolerance in Caveolin-1 Deficient Mice
    Article Snippet: Samples were immunoprecipitated using streptavidin conjugated antibodies (Dynabeads M-280 Streptavidin from Invitrogen). .. Biotinylated Cav-1 was resolved by non-reducing SDS-polyacrylamide gel electrophoresis (PAGE), transferred and detected by immunoblotting with mouse anti-Cav-1 (1∶1000).

    Biotin Switch Assay:

    Article Title: Nitroglycerin Tolerance in Caveolin-1 Deficient Mice
    Article Snippet: Paragraph title: Biotin switch assay ... Samples were immunoprecipitated using streptavidin conjugated antibodies (Dynabeads M-280 Streptavidin from Invitrogen).

    Activated Clotting Time Assay:

    Article Title: Active nematic emulsions
    Article Snippet: MTs were mixed with the molecular motor clusters that act as active cross-linkers and with ATP (A2383, Sigma) that drives the activity of the gel. .. To maintain a constant concentration of ATP during the experiments, we added an enzymatic ATP-regenerator system based on phosphoenolpyruvate (P7127, Sigma) and pyruvate kinase (434301, Invitrogen), which converted adenosine 5′-diphosphate back into ATP.

    Purification:

    Article Title: Rapid Microsphere Assay for Identification of Cryptosporidium hominis and Cryptosporidium parvum in Stool and Environmental Samples ▿
    Article Snippet: Post-PCR purification was not required prior to hybridization. .. After incubation, the plate was centrifuged at 3,580 × g for 5 min, 25 μl of supernatant was carefully removed, and 75 μl of a 1:40 dilution of streptavidin-R-phycoerythrin (SA-PE) (Molecular Probes, Eugene, OR) was added.

    Software:

    Article Title: DNA Suspension Arrays: Silencing Discrete Artifacts for High-Sensitivity Applications
    Article Snippet: Bead and Streptavidin-R-Phycoerythrin Hybridization, Flow Fluorimetry Approximately 750 of each xTAG bead, competimer and/or DNO (where indicated) were added to each LDR in 1X TMAC buffer (2.5 M tetramethylammonium chloride (TMAC), 0.1 M Tris-HCl (pH 8.0), 3 mM ethylenediaminetetraacetic acid and 0.1% sodium dodecyl sulfate) in 60 µl and subjected to 95°C (5 min.) and 37°C (45 min.), followed by addition of a Streptavidin-R-phycoerythrin conjugate (100 ng/well; Invitrogen #S-866) in 6 µl 1X TMAC buffer and incubation at 37°C (45 min.). .. Seventy-five beads per region were measured on a Luminex 200 instrument with BioPlex Manager software (Bio-Rad Life Sciences, Hercules, CA) at the highest reporter channel photomultiplier tube voltage setting (typically 700–750 V).

    Article Title: Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens
    Article Snippet: The development protocol using biotinylated, monoclonal anti-IgG (clone H4; Southern Biotech) and anti-IgG4 (clone HP6025; Zymed) antibodies and R -phycoerythrin-labeled streptavidin (Invitrogen) has been previously described ( ). .. After the final wash step, the beads in each well were suspended in 125 μl PBS, and the plate was analyzed using a Bio-Plex 200 or 100 instrument (Bio-Rad, Hercules, CA) equipped with Bio-Plex Manager 6.0 software (Bio-Rad).

    Multiplex Assay:

    Article Title: Multiplex Assay Detection of Immunoglobulin G Antibodies That Recognize Babesia microti Antigens
    Article Snippet: Paragraph title: Multiplex bead assay (MBA). ... The development protocol using biotinylated, monoclonal anti-IgG (clone H4; Southern Biotech) and anti-IgG4 (clone HP6025; Zymed) antibodies and R -phycoerythrin-labeled streptavidin (Invitrogen) has been previously described ( ).

    Sample Prep:

    Article Title: Single Molecule FRET of Protein-Nucleic Acid and Protein-Protein complexes: Surface Passivation and Immobilization
    Article Snippet: A step-by-step protocol for sample preparation and immobilization is detailed below. .. Inject a streptavidin solution (Molecular Probes S888, 0.2 mg/mL in 50 mM Tris-HCl, pH 7.5, 50 mM NaCl) and incubate for 5–10 minutes to allow it to interact with biotin ( ).

    Immunoprecipitation:

    Article Title: Nitroglycerin Tolerance in Caveolin-1 Deficient Mice
    Article Snippet: .. Samples were immunoprecipitated using streptavidin conjugated antibodies (Dynabeads M-280 Streptavidin from Invitrogen). .. Biotinylated Cav-1 was resolved by non-reducing SDS-polyacrylamide gel electrophoresis (PAGE), transferred and detected by immunoblotting with mouse anti-Cav-1 (1∶1000).

    Lysis:

    Article Title: T cell-specific FADD-deficient mice: FADD is required for early T cell development
    Article Snippet: In brief, thymocytes, lymph node cells, and splenocytes were harvested and depleted of erythrocytes by treatment for 5 min at room temperature with red blood cell lysis buffer (Sigma). .. CD4, CD8, B220, and streptavidin-tricolor antibodies were purchased from Caltag.

    Staining:

    Article Title: The anti-apoptotic activities of Rel and RelA required during B-cell maturation involve the regulation of Bcl-2 expression
    Article Snippet: .. Staining with biotinylated antibodies was revealed by R-phycoerythrin–streptavidin or tricolor–streptavidin (Caltag, San Francisco, CA). .. Between 5000 and 10 000 viable cells were analyzed using a FACScan flow cytometer (Becton Dickinson, San Jose, CA).

    Article Title: B Lymphocytes Differentially Use the Rel and Nuclear Factor ?B1 (NF-?B1) Transcription Factors to Regulate Cell Cycle Progression and Apoptosis in Quiescent and Mitogen-activated Cells
    Article Snippet: .. Binding of biotinylated antibodies was revealed by staining with R-phycoerythrin-streptavidin (CALTAG Labs.) as the secondary reagent. .. Viable (PI negative) cells (5,000–10,000) were analyzed in a FACScan® flow cytometer ( Becton Dickinson ).

    Article Title: T cell-specific FADD-deficient mice: FADD is required for early T cell development
    Article Snippet: One million peripheral lymphoid cells were incubated with anti-FcγII/III receptor antibody (2.4G2, PharMingen) and normal rabbit serum for 10 min 4°C before cell surface staining. .. CD4, CD8, B220, and streptavidin-tricolor antibodies were purchased from Caltag.

    Luminex:

    Article Title: DNA Suspension Arrays: Silencing Discrete Artifacts for High-Sensitivity Applications
    Article Snippet: Bead and Streptavidin-R-Phycoerythrin Hybridization, Flow Fluorimetry Approximately 750 of each xTAG bead, competimer and/or DNO (where indicated) were added to each LDR in 1X TMAC buffer (2.5 M tetramethylammonium chloride (TMAC), 0.1 M Tris-HCl (pH 8.0), 3 mM ethylenediaminetetraacetic acid and 0.1% sodium dodecyl sulfate) in 60 µl and subjected to 95°C (5 min.) and 37°C (45 min.), followed by addition of a Streptavidin-R-phycoerythrin conjugate (100 ng/well; Invitrogen #S-866) in 6 µl 1X TMAC buffer and incubation at 37°C (45 min.). .. Seventy-five beads per region were measured on a Luminex 200 instrument with BioPlex Manager software (Bio-Rad Life Sciences, Hercules, CA) at the highest reporter channel photomultiplier tube voltage setting (typically 700–750 V).

    Article Title: Rapid Microsphere Assay for Identification of Cryptosporidium hominis and Cryptosporidium parvum in Stool and Environmental Samples ▿
    Article Snippet: After incubation, the plate was centrifuged at 3,580 × g for 5 min, 25 μl of supernatant was carefully removed, and 75 μl of a 1:40 dilution of streptavidin-R-phycoerythrin (SA-PE) (Molecular Probes, Eugene, OR) was added. .. The plate was incubated for 15 min at 42°C and read on a heated plate at the same temperature on the Luminex 100 platform.

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  • 78
    Thermo Fisher immunopure biotinylated mouse
    Combined DNA and protein microarrays on the same slide. ( A ) Example of a typical response obtained in a combined microarray using DNA and antibodies. The three spots at the top are molecular beacons for pag and the three bottom spots of <t>Immunopure™</t> <t>biotinylated</t> mouse anti-human IgG. ( B ) Time course of the microarray response to bioanalyte solutions. First DNA from pag was applied (green arrow), followed by the secondary antibody (Alexa Fluor 532-labeled goat anti-m ouse IgG, red arrow). Notice that even though the secondary antibody floods the entire chamber, only the spots containing the primary antibody produced a fluorescent signal. This simple experiment demonstrates the power of surface selectivity obtained with LG-TIRFM-MB. Microarray reconstitution brings the fluorescence towards baseline (pre stimulation) levels. At the right of the figure is shown the fluorescence intensity scale. ( C ) Lines depict time courses showing changes in mean fluorescence of each spot over time. Time points where DNA and antibody were applied are indicated by the arrows.
    Immunopure Biotinylated Mouse, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 78/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopure biotinylated mouse/product/Thermo Fisher
    Average 78 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunopure biotinylated mouse - by Bioz Stars, 2020-03
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    99
    Thermo Fisher streptavidin
    SPR sensorgram of <t>streptavidin-aptamer</t> binding. Streptavidin was covalently immobilized on a CM5-chip. Aptamer clone R10#17 with highest affinity determined by FLAA was injected at a concentrations ranging between 0.1 and 2 µM in binding buffer.
    Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 169 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/streptavidin/product/Thermo Fisher
    Average 99 stars, based on 169 article reviews
    Price from $9.99 to $1999.99
    streptavidin - by Bioz Stars, 2020-03
    99/100 stars
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    79
    Thermo Fisher immunopurer streptavidin
    SPR sensorgram of <t>streptavidin-aptamer</t> binding. Streptavidin was covalently immobilized on a CM5-chip. Aptamer clone R10#17 with highest affinity determined by FLAA was injected at a concentrations ranging between 0.1 and 2 µM in binding buffer.
    Immunopurer Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 79/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopurer streptavidin/product/Thermo Fisher
    Average 79 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunopurer streptavidin - by Bioz Stars, 2020-03
    79/100 stars
      Buy from Supplier

    99
    Thermo Fisher immunopure streptavidin
    SPR sensorgram of <t>streptavidin-aptamer</t> binding. Streptavidin was covalently immobilized on a CM5-chip. Aptamer clone R10#17 with highest affinity determined by FLAA was injected at a concentrations ranging between 0.1 and 2 µM in binding buffer.
    Immunopure Streptavidin, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 6 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunopure streptavidin/product/Thermo Fisher
    Average 99 stars, based on 6 article reviews
    Price from $9.99 to $1999.99
    immunopure streptavidin - by Bioz Stars, 2020-03
    99/100 stars
      Buy from Supplier

    Image Search Results


    Combined DNA and protein microarrays on the same slide. ( A ) Example of a typical response obtained in a combined microarray using DNA and antibodies. The three spots at the top are molecular beacons for pag and the three bottom spots of Immunopure™ biotinylated mouse anti-human IgG. ( B ) Time course of the microarray response to bioanalyte solutions. First DNA from pag was applied (green arrow), followed by the secondary antibody (Alexa Fluor 532-labeled goat anti-m ouse IgG, red arrow). Notice that even though the secondary antibody floods the entire chamber, only the spots containing the primary antibody produced a fluorescent signal. This simple experiment demonstrates the power of surface selectivity obtained with LG-TIRFM-MB. Microarray reconstitution brings the fluorescence towards baseline (pre stimulation) levels. At the right of the figure is shown the fluorescence intensity scale. ( C ) Lines depict time courses showing changes in mean fluorescence of each spot over time. Time points where DNA and antibody were applied are indicated by the arrows.

    Journal: Sensors (Basel, Switzerland)

    Article Title: A Platform for Combined DNA and Protein Microarrays Based on Total Internal Reflection Fluorescence

    doi: 10.3390/s120201800

    Figure Lengend Snippet: Combined DNA and protein microarrays on the same slide. ( A ) Example of a typical response obtained in a combined microarray using DNA and antibodies. The three spots at the top are molecular beacons for pag and the three bottom spots of Immunopure™ biotinylated mouse anti-human IgG. ( B ) Time course of the microarray response to bioanalyte solutions. First DNA from pag was applied (green arrow), followed by the secondary antibody (Alexa Fluor 532-labeled goat anti-m ouse IgG, red arrow). Notice that even though the secondary antibody floods the entire chamber, only the spots containing the primary antibody produced a fluorescent signal. This simple experiment demonstrates the power of surface selectivity obtained with LG-TIRFM-MB. Microarray reconstitution brings the fluorescence towards baseline (pre stimulation) levels. At the right of the figure is shown the fluorescence intensity scale. ( C ) Lines depict time courses showing changes in mean fluorescence of each spot over time. Time points where DNA and antibody were applied are indicated by the arrows.

    Article Snippet: Immunopure™ biotinylated mouse (antibody 1) and rabbit (antibody 2) anti-human IgG were purchased from ThermoScientific (cat# 31784 and 31786, respectively), and reconstituted to 1.5 mg/mL according to the manufacturer’s guidelines and stored at 4 °C.

    Techniques: Microarray, Labeling, Produced, Fluorescence

    SPR sensorgram of streptavidin-aptamer binding. Streptavidin was covalently immobilized on a CM5-chip. Aptamer clone R10#17 with highest affinity determined by FLAA was injected at a concentrations ranging between 0.1 and 2 µM in binding buffer.

    Journal: PLoS ONE

    Article Title: Probing the SELEX Process with Next-Generation Sequencing

    doi: 10.1371/journal.pone.0029604

    Figure Lengend Snippet: SPR sensorgram of streptavidin-aptamer binding. Streptavidin was covalently immobilized on a CM5-chip. Aptamer clone R10#17 with highest affinity determined by FLAA was injected at a concentrations ranging between 0.1 and 2 µM in binding buffer.

    Article Snippet: After the injection of 10 µl 10 µg/ml Streptavidin (ImmunoPure® Streptavidin, Thermo Scientific, Bonn, Germany) in 10 mM sodium acetate (pH 4), 35 µl of 1 M ethanolamine (pH 8.5) were used to block remaining reactive groups followed by two washing steps.

    Techniques: SPR Assay, Binding Assay, Chromatin Immunoprecipitation, Injection

    Microtiter plate binding assay (FLAA) of selection pools to immobilized streptavidin. Depicted are rounds 1 to 10 with the original library pool indicated as round 0.

    Journal: PLoS ONE

    Article Title: Probing the SELEX Process with Next-Generation Sequencing

    doi: 10.1371/journal.pone.0029604

    Figure Lengend Snippet: Microtiter plate binding assay (FLAA) of selection pools to immobilized streptavidin. Depicted are rounds 1 to 10 with the original library pool indicated as round 0.

    Article Snippet: After the injection of 10 µl 10 µg/ml Streptavidin (ImmunoPure® Streptavidin, Thermo Scientific, Bonn, Germany) in 10 mM sodium acetate (pH 4), 35 µl of 1 M ethanolamine (pH 8.5) were used to block remaining reactive groups followed by two washing steps.

    Techniques: Binding Assay, Selection

    Microtiter plate binding assay (FLAA) of single clones applied as synthetic oligonucleotides to immobilized streptavidin.

    Journal: PLoS ONE

    Article Title: Probing the SELEX Process with Next-Generation Sequencing

    doi: 10.1371/journal.pone.0029604

    Figure Lengend Snippet: Microtiter plate binding assay (FLAA) of single clones applied as synthetic oligonucleotides to immobilized streptavidin.

    Article Snippet: After the injection of 10 µl 10 µg/ml Streptavidin (ImmunoPure® Streptavidin, Thermo Scientific, Bonn, Germany) in 10 mM sodium acetate (pH 4), 35 µl of 1 M ethanolamine (pH 8.5) were used to block remaining reactive groups followed by two washing steps.

    Techniques: Binding Assay, Clone Assay