immunohistochemical stainer  (Ventana Medical)

 
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    Ventana Medical immunohistochemical stainer
    Semi-quantitative <t>immunohistochemical</t> scoring of CD20 expression. The images of weak and incomplete (A, upper), weak and complete (B, upper), and strong and complete (C, upper) staining for CD20 are digitally analyzed using the free web-based ImmunoMembrane (IM) plug-in that produces a combined score of 2 (A, lower), 9 (B, lower), and 20 (C, lower) in its pseudo-color image, respectively.
    Immunohistochemical Stainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    immunohistochemical stainer - by Bioz Stars, 2021-04
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    Images

    1) Product Images from "Prognostic Implication of Semi-quantitative Immunohistochemical Assessment of CD20 Expression in Diffuse Large B-Cell Lymphoma"

    Article Title: Prognostic Implication of Semi-quantitative Immunohistochemical Assessment of CD20 Expression in Diffuse Large B-Cell Lymphoma

    Journal: Journal of Pathology and Translational Medicine

    doi: 10.4132/jptm.2016.01.12

    Semi-quantitative immunohistochemical scoring of CD20 expression. The images of weak and incomplete (A, upper), weak and complete (B, upper), and strong and complete (C, upper) staining for CD20 are digitally analyzed using the free web-based ImmunoMembrane (IM) plug-in that produces a combined score of 2 (A, lower), 9 (B, lower), and 20 (C, lower) in its pseudo-color image, respectively.
    Figure Legend Snippet: Semi-quantitative immunohistochemical scoring of CD20 expression. The images of weak and incomplete (A, upper), weak and complete (B, upper), and strong and complete (C, upper) staining for CD20 are digitally analyzed using the free web-based ImmunoMembrane (IM) plug-in that produces a combined score of 2 (A, lower), 9 (B, lower), and 20 (C, lower) in its pseudo-color image, respectively.

    Techniques Used: Immunohistochemistry, Expressing, Staining

    Reproducibility of the immunohistochemical scoring. The combined scores of CD20 expression obtained from the first run of semi-quantitative immunohistochemical scoring are reproducible in the second run. Yellow triangle, CD20-low group; blue triangle, CD20-high group; red rhombus, germinal center of the tonsil as control tissue.
    Figure Legend Snippet: Reproducibility of the immunohistochemical scoring. The combined scores of CD20 expression obtained from the first run of semi-quantitative immunohistochemical scoring are reproducible in the second run. Yellow triangle, CD20-low group; blue triangle, CD20-high group; red rhombus, germinal center of the tonsil as control tissue.

    Techniques Used: Immunohistochemistry, Expressing

    2) Product Images from "Activation of MEK1/2‐ERK1/2 signaling during NNK‐induced lung carcinogenesis in female A/J mice"

    Article Title: Activation of MEK1/2‐ERK1/2 signaling during NNK‐induced lung carcinogenesis in female A/J mice

    Journal: Cancer Medicine

    doi: 10.1002/cam4.652

    Immunohistochemical expression of phosphorylated extracellular signal‐regulated kinase 1/2 ( ERK1/2) (pERK1/2) in 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanone ‐induced mouse lung tumors. (A) Immunohistochemical staining pattern for pERK1/2 with tumor progression: (a) surrounding normal tissue (N) of a tumor (T); (b) hyperplasia; (c) adenoma, negative for pERK1/2; (d) adenocarcinoma intensely stained for pERK1/2, in both nuclei and cytoplasm. Magnification ×200 (a, b); ×100 (c, d); ×400 inset (c, d). (B) Scores of staining intensity, number of positive cells and their multiplication (positive score) for pERK1/2 in hyperplasia (H), adenoma (AD) and adenocarcinoma (ADC). Shown are averages and standard deviations (error bars). ** P
    Figure Legend Snippet: Immunohistochemical expression of phosphorylated extracellular signal‐regulated kinase 1/2 ( ERK1/2) (pERK1/2) in 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanone ‐induced mouse lung tumors. (A) Immunohistochemical staining pattern for pERK1/2 with tumor progression: (a) surrounding normal tissue (N) of a tumor (T); (b) hyperplasia; (c) adenoma, negative for pERK1/2; (d) adenocarcinoma intensely stained for pERK1/2, in both nuclei and cytoplasm. Magnification ×200 (a, b); ×100 (c, d); ×400 inset (c, d). (B) Scores of staining intensity, number of positive cells and their multiplication (positive score) for pERK1/2 in hyperplasia (H), adenoma (AD) and adenocarcinoma (ADC). Shown are averages and standard deviations (error bars). ** P

    Techniques Used: Immunohistochemistry, Expressing, Staining

    Immunohistochemical expression of phosphorylated MEK1/2 (pMEK1/2) in 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanone ‐induced mouse lung tumors. (A) Immunohistochemical staining pattern of pMEK1/2 with tumor progression: (a) surrounding normal tissue (N) of a tumor (T); (b) hyperplasia negative or weakly positive with pMEK1/2; (C) adenoma; (D) adenocarcinoma moderate or strongly positive for pMEK1/2. Magnification ×200 (a, b); ×100 (c, d); ×400 inset (c, d). (B) Scores of staining intensity, numbers of positive cells and their multiplication (positive score) for pMEK1/2 in hyperplasia (H), adenoma (AD) and adenocarcinoma (ADC). Shown are averages and standard deviations (error bars). ** P
    Figure Legend Snippet: Immunohistochemical expression of phosphorylated MEK1/2 (pMEK1/2) in 4‐(methylnitrosamino)‐1‐(3‐pyridyl)‐1‐butanone ‐induced mouse lung tumors. (A) Immunohistochemical staining pattern of pMEK1/2 with tumor progression: (a) surrounding normal tissue (N) of a tumor (T); (b) hyperplasia negative or weakly positive with pMEK1/2; (C) adenoma; (D) adenocarcinoma moderate or strongly positive for pMEK1/2. Magnification ×200 (a, b); ×100 (c, d); ×400 inset (c, d). (B) Scores of staining intensity, numbers of positive cells and their multiplication (positive score) for pMEK1/2 in hyperplasia (H), adenoma (AD) and adenocarcinoma (ADC). Shown are averages and standard deviations (error bars). ** P

    Techniques Used: Immunohistochemistry, Expressing, Staining

    3) Product Images from "Thioredoxin interacting protein promotes invasion in hepatocellular carcinoma"

    Article Title: Thioredoxin interacting protein promotes invasion in hepatocellular carcinoma

    Journal: Oncotarget

    doi: 10.18632/oncotarget.26402

    TXNIP levels in normal, cirrhotic and HCC tumor samples ( A , B ) TXNIP expression levels were determined in both primary HCC tumor samples and their adjacent non-tumor tissue as well as in normal and cirrhotic liver tissues by IHC analysis. ( C , D ) TXNIP staining patterns were analyzed in primary HCC tumor tissues and surrounding non-tumoral tissues. NS: not significant, * p > 0.05, *** p
    Figure Legend Snippet: TXNIP levels in normal, cirrhotic and HCC tumor samples ( A , B ) TXNIP expression levels were determined in both primary HCC tumor samples and their adjacent non-tumor tissue as well as in normal and cirrhotic liver tissues by IHC analysis. ( C , D ) TXNIP staining patterns were analyzed in primary HCC tumor tissues and surrounding non-tumoral tissues. NS: not significant, * p > 0.05, *** p

    Techniques Used: Expressing, Immunohistochemistry, Staining

    4) Product Images from "Programmed death-ligand 1 is upregulated in intrahepatic lymphoepithelioma-like cholangiocarcinoma"

    Article Title: Programmed death-ligand 1 is upregulated in intrahepatic lymphoepithelioma-like cholangiocarcinoma

    Journal: Oncotarget

    doi: 10.18632/oncotarget.11949

    Representative images of PD-L1 immunostaining PD-L1 was immunostained on the membrane and/or in the cytoplasm of tumor cells with variable intensities: ( A – D ) absent in the conventional IHCC (score 0), weak in LELCC (score 1), moderate in LELCC (score 2), strong in LELCC (score 3). In most cases, PD-L1 was expressed in tumor cells, which were highlighted by ( E ) AE1/AE3, and ( F ) tumor-infiltrated immune cells. ( H ) In 3 cases, PD-L1 was immunostained mainly in tumor-infiltrated immune cells, ( G ) with little staining in tumor cells, which were highlighted by AE1/AE3. Immunohistochemistry, ×200.
    Figure Legend Snippet: Representative images of PD-L1 immunostaining PD-L1 was immunostained on the membrane and/or in the cytoplasm of tumor cells with variable intensities: ( A – D ) absent in the conventional IHCC (score 0), weak in LELCC (score 1), moderate in LELCC (score 2), strong in LELCC (score 3). In most cases, PD-L1 was expressed in tumor cells, which were highlighted by ( E ) AE1/AE3, and ( F ) tumor-infiltrated immune cells. ( H ) In 3 cases, PD-L1 was immunostained mainly in tumor-infiltrated immune cells, ( G ) with little staining in tumor cells, which were highlighted by AE1/AE3. Immunohistochemistry, ×200.

    Techniques Used: Immunostaining, Staining, Immunohistochemistry

    Immunohistochemical features of lymphoepithelioma-like cholangiocarcinoma The adenocarcinoma component is diffusely positive for ( A ) CK7 and ( B ) CK19, while the lymphoepithelioma-like carcinoma component is focally positive for ( C ) CK7 and ( D ) CK19. ( E ) The tumor cells of both components were negative for HepPar-1. Immunohistochemistry, ×200.
    Figure Legend Snippet: Immunohistochemical features of lymphoepithelioma-like cholangiocarcinoma The adenocarcinoma component is diffusely positive for ( A ) CK7 and ( B ) CK19, while the lymphoepithelioma-like carcinoma component is focally positive for ( C ) CK7 and ( D ) CK19. ( E ) The tumor cells of both components were negative for HepPar-1. Immunohistochemistry, ×200.

    Techniques Used: Immunohistochemistry

    5) Product Images from "Tubular Adenoma of the Breast: A Clinicopathologic Study of a Series of 9 Cases"

    Article Title: Tubular Adenoma of the Breast: A Clinicopathologic Study of a Series of 9 Cases

    Journal: Clinical Medicine Insights. Pathology

    doi: 10.1177/1179555718757499

    CD34 immunostaining highlights the characteristic small amount of the stroma intermingled between the tubular structures (original magnification ×100).
    Figure Legend Snippet: CD34 immunostaining highlights the characteristic small amount of the stroma intermingled between the tubular structures (original magnification ×100).

    Techniques Used: Immunostaining

    Immunohistochemical analysis shows a prominent expression of CK5/6 (A) and p63 protein (B) by tumors cells (original magnification x200).
    Figure Legend Snippet: Immunohistochemical analysis shows a prominent expression of CK5/6 (A) and p63 protein (B) by tumors cells (original magnification x200).

    Techniques Used: Immunohistochemistry, Expressing

    6) Product Images from "Cytokeratin 17 and Ki-67: Immunohistochemical markers for the differential diagnosis of keratoacanthoma and squamous cell carcinoma"

    Article Title: Cytokeratin 17 and Ki-67: Immunohistochemical markers for the differential diagnosis of keratoacanthoma and squamous cell carcinoma

    Journal: Oncology Letters

    doi: 10.3892/ol.2017.5793

    Representative images of (A) hematoxylin and eosin-stained SCC (magnification, 100) exhibiting (B) a proliferation marker protein Ki-67 IHC DP (magnification, ×200), and a cytokeratin 17 IHC DP at (C) magnification ×100 and (D) magnification ×200. These staining patterns make up the SCC-like staining pattern. SCC, squamous cell carcinoma. DP, diffuse staining pattern; IHC, immunohistochemistry.
    Figure Legend Snippet: Representative images of (A) hematoxylin and eosin-stained SCC (magnification, 100) exhibiting (B) a proliferation marker protein Ki-67 IHC DP (magnification, ×200), and a cytokeratin 17 IHC DP at (C) magnification ×100 and (D) magnification ×200. These staining patterns make up the SCC-like staining pattern. SCC, squamous cell carcinoma. DP, diffuse staining pattern; IHC, immunohistochemistry.

    Techniques Used: Staining, Marker, Immunohistochemistry

    Representative images of crateriform SCC. Representative images of (A) the H E-stained crateriform architecture (magnification, ×40), (B) H E-stained actinic keratosis adjacent to the lesion (magnification, ×100), (C) H E-stained cytological atypia (magnification, ×200), (D) an infiltrative H E staining pattern (magnification, ×100), and an SCC-like (E) cytokeratin 17 (magnification, ×100) or (F) proliferation marker protein Ki-67 (magnification, ×100) immunohistochemical staining pattern. SCC, squamous cell carcinoma; H E, hematoxylin and eosin.
    Figure Legend Snippet: Representative images of crateriform SCC. Representative images of (A) the H E-stained crateriform architecture (magnification, ×40), (B) H E-stained actinic keratosis adjacent to the lesion (magnification, ×100), (C) H E-stained cytological atypia (magnification, ×200), (D) an infiltrative H E staining pattern (magnification, ×100), and an SCC-like (E) cytokeratin 17 (magnification, ×100) or (F) proliferation marker protein Ki-67 (magnification, ×100) immunohistochemical staining pattern. SCC, squamous cell carcinoma; H E, hematoxylin and eosin.

    Techniques Used: Staining, Marker, Immunohistochemistry

    Representative images of hematoxylin and eosin-stained keratoacanthoma, at (A) magnification, ×40 and (B) magnification, ×100, exhibiting (C) a peripheral proliferation marker protein Ki-67 IHC staining pattern (magnification, ×100) or a central CK17 IHC staining pattern at (D) magnification, ×40, and magnification, ×200 in (E) one field and vision and (F) another field of vision. Red arrowhead, no CK17 staining of basal cells; black arrowhead, weak CK17 staining of basal cells. CK17, cytokeratin 17; IHC, immunohistochemistry.
    Figure Legend Snippet: Representative images of hematoxylin and eosin-stained keratoacanthoma, at (A) magnification, ×40 and (B) magnification, ×100, exhibiting (C) a peripheral proliferation marker protein Ki-67 IHC staining pattern (magnification, ×100) or a central CK17 IHC staining pattern at (D) magnification, ×40, and magnification, ×200 in (E) one field and vision and (F) another field of vision. Red arrowhead, no CK17 staining of basal cells; black arrowhead, weak CK17 staining of basal cells. CK17, cytokeratin 17; IHC, immunohistochemistry.

    Techniques Used: Staining, Marker, Immunohistochemistry

    Representative images of (A) hematoxylin and eosin-stained KA (magnification, ×100) exhibiting a central CK17 IHC staining pattern at (B) magnification, ×100 and (C) magnification, ×200, or (D) a peripheral proliferation marker protein Ki-67 IHC staining pattern (magnification, ×100). These staining patterns constitute the KA-like staining pattern, exhibiting no basal or peripheral CK17 staining. KA, keratoacanthoma; CK17, cytokeratin 17; IHC, immunohistochemistry.
    Figure Legend Snippet: Representative images of (A) hematoxylin and eosin-stained KA (magnification, ×100) exhibiting a central CK17 IHC staining pattern at (B) magnification, ×100 and (C) magnification, ×200, or (D) a peripheral proliferation marker protein Ki-67 IHC staining pattern (magnification, ×100). These staining patterns constitute the KA-like staining pattern, exhibiting no basal or peripheral CK17 staining. KA, keratoacanthoma; CK17, cytokeratin 17; IHC, immunohistochemistry.

    Techniques Used: Staining, Immunohistochemistry, Marker

    Representative images of the suprabasal cells of the ORS. (A) The normal hair follicle and sebaceous gland cells stain positively for CK17, while the epidermal cells do not (magnification, ×100). The majority of ORS basal cells (B) stain weakly for CK17 (magnification, ×200) or (C) do not stain at all (magnification, ×100). Immunohistochemical staining used for all. ORS, outer root sheath.
    Figure Legend Snippet: Representative images of the suprabasal cells of the ORS. (A) The normal hair follicle and sebaceous gland cells stain positively for CK17, while the epidermal cells do not (magnification, ×100). The majority of ORS basal cells (B) stain weakly for CK17 (magnification, ×200) or (C) do not stain at all (magnification, ×100). Immunohistochemical staining used for all. ORS, outer root sheath.

    Techniques Used: Staining, Immunohistochemistry

    7) Product Images from "In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks"

    Article Title: In-house Manual Construction of High-Density and High-Quality Tissue Microarrays by Using Homemade Recipient Agarose-Paraffin Blocks

    Journal: Korean Journal of Pathology

    doi: 10.4132/KoreanJPathol.2013.47.3.238

    Manual construction of a tissue microarray (TMA) with a 1 mm core diameter. (A, B) Tissue punches made of a modified 18 ( * )- and 16 ( ** )-gauge needle are used to create holes in the recipient agarose-paraffin block, punch donor tissue cores and implant them into the pre-made holes. Once the TMA is completed (C), the TMA is detached from the cassette (D, E) and placed in the embedding mold in order to be re-melted and re-embedded in paraffin (F-H). The TMA block is trimmed (I) and cut for hematoxylin and eosin (J) and immunohistochemical staining (K, cytokeratin; L, CD20).
    Figure Legend Snippet: Manual construction of a tissue microarray (TMA) with a 1 mm core diameter. (A, B) Tissue punches made of a modified 18 ( * )- and 16 ( ** )-gauge needle are used to create holes in the recipient agarose-paraffin block, punch donor tissue cores and implant them into the pre-made holes. Once the TMA is completed (C), the TMA is detached from the cassette (D, E) and placed in the embedding mold in order to be re-melted and re-embedded in paraffin (F-H). The TMA block is trimmed (I) and cut for hematoxylin and eosin (J) and immunohistochemical staining (K, cytokeratin; L, CD20).

    Techniques Used: Microarray, Modification, Blocking Assay, Immunohistochemistry, Staining

    8) Product Images from "Hypoxia Pathways and Cellular Stress Activate Pancreatic Stellate Cells: Development of an Organotypic Culture Model of Thick Slices of Normal Human Pancreas"

    Article Title: Hypoxia Pathways and Cellular Stress Activate Pancreatic Stellate Cells: Development of an Organotypic Culture Model of Thick Slices of Normal Human Pancreas

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0076229

    Immunohistochemical expression of caspase-3 antibody at baseline (a), H24 (3b), H48 (3c) and H72 (3d) in hyperoxic conditions. At H72 (3d), less than 5 cells per HPF expressed the caspase-3. Other types of cells (islets, ductal) did not express caspase-3 at any time. The membranous CA9 and the nuclear Hif-1α expression in acinar cells was studied at baseline (3e and 3i), H24 (3f and 3j), H48 (3g and 3k) and H72 (3h and 3l). The intensity greatly increased in dedifferentiated areas with a gradual appearance.
    Figure Legend Snippet: Immunohistochemical expression of caspase-3 antibody at baseline (a), H24 (3b), H48 (3c) and H72 (3d) in hyperoxic conditions. At H72 (3d), less than 5 cells per HPF expressed the caspase-3. Other types of cells (islets, ductal) did not express caspase-3 at any time. The membranous CA9 and the nuclear Hif-1α expression in acinar cells was studied at baseline (3e and 3i), H24 (3f and 3j), H48 (3g and 3k) and H72 (3h and 3l). The intensity greatly increased in dedifferentiated areas with a gradual appearance.

    Techniques Used: Immunohistochemistry, Expressing

    Nestin expression of PSC (black arrows) at baseline (8a), H24 (8b), H48 (8c) and H72 (8d). Smooth muscle actin expression of PSC (black arrows) at baseline (8e), H24 (8f), H48 (8g) and H72 (8h). Double Ki67/α-SMA expression of PSC was performed. The nuclear Ki67 and the cytoplasmic α-SMA expression (black arrows) appeared as red and brown immunostaining, respectively (baseline, 8i; H24, 8j; H48, 8 k and H72, 8l). The Ki67 nuclear staining of PSC was observed (baseline, 8m; H24, 8o; H48, 8p and H72, 8q).
    Figure Legend Snippet: Nestin expression of PSC (black arrows) at baseline (8a), H24 (8b), H48 (8c) and H72 (8d). Smooth muscle actin expression of PSC (black arrows) at baseline (8e), H24 (8f), H48 (8g) and H72 (8h). Double Ki67/α-SMA expression of PSC was performed. The nuclear Ki67 and the cytoplasmic α-SMA expression (black arrows) appeared as red and brown immunostaining, respectively (baseline, 8i; H24, 8j; H48, 8 k and H72, 8l). The Ki67 nuclear staining of PSC was observed (baseline, 8m; H24, 8o; H48, 8p and H72, 8q).

    Techniques Used: Expressing, Immunostaining, Staining

    9) Product Images from "γH2AX is immunohistochemically detectable until 7 days after exposure of N-bis (2-hydroxypropyl) nitrosamine (DHPN) in rat lung carcinogenesis"

    Article Title: γH2AX is immunohistochemically detectable until 7 days after exposure of N-bis (2-hydroxypropyl) nitrosamine (DHPN) in rat lung carcinogenesis

    Journal: Journal of Toxicologic Pathology

    doi: 10.1293/tox.2017-0066

    Immunohistochemical findings of the lungs at 3 days. The images in A and B are for γH2AX, and those in C and D are for PCNA. Positive cells are indicated by brown staining of the nucleus. Randomly selected slides of lung sections under ×400 magnification. A DHPN-treated group is shown in A and C, and a control group is shown in B and D.
    Figure Legend Snippet: Immunohistochemical findings of the lungs at 3 days. The images in A and B are for γH2AX, and those in C and D are for PCNA. Positive cells are indicated by brown staining of the nucleus. Randomly selected slides of lung sections under ×400 magnification. A DHPN-treated group is shown in A and C, and a control group is shown in B and D.

    Techniques Used: Immunohistochemistry, Staining

    10) Product Images from "Differential expression of Caveolin-1 in hepatocellular carcinoma: correlation with differentiation state, motility and invasion"

    Article Title: Differential expression of Caveolin-1 in hepatocellular carcinoma: correlation with differentiation state, motility and invasion

    Journal: BMC Cancer

    doi: 10.1186/1471-2407-9-65

    Immunohistochemical staining of Cav-1 in normal, cirrhotic and HCC tissue sections . (A) Absence of Cav-1 expression in hepatocytes of normal donor liver tissue and strong Cav-1 expression in the endothelium of the blood vessels. (B) Cav-1 positivity in the cirrhotic nodule and endothelium of the vessels with same intensity. (C) Membranous Cav-1 positivity in one of the well differentiated HCC cases. (D) Strong cytoplasmic Cav-1 expression in a poorly differentiated HCC. (E) Staining of Cav-1 in an HCC sample with its tumoral and surrounding peritumoral region. (F) The percentage of Cav-1 staining in normal liver, cirrhosis and HCC tissues (* p
    Figure Legend Snippet: Immunohistochemical staining of Cav-1 in normal, cirrhotic and HCC tissue sections . (A) Absence of Cav-1 expression in hepatocytes of normal donor liver tissue and strong Cav-1 expression in the endothelium of the blood vessels. (B) Cav-1 positivity in the cirrhotic nodule and endothelium of the vessels with same intensity. (C) Membranous Cav-1 positivity in one of the well differentiated HCC cases. (D) Strong cytoplasmic Cav-1 expression in a poorly differentiated HCC. (E) Staining of Cav-1 in an HCC sample with its tumoral and surrounding peritumoral region. (F) The percentage of Cav-1 staining in normal liver, cirrhosis and HCC tissues (* p

    Techniques Used: Immunohistochemistry, Staining, Expressing

    11) Product Images from "Programmed death‐ligand 1 expression in gastric cancer: correlation with mismatch repair deficiency and HER2‐negative status"

    Article Title: Programmed death‐ligand 1 expression in gastric cancer: correlation with mismatch repair deficiency and HER2‐negative status

    Journal: Cancer Medicine

    doi: 10.1002/cam4.1502

    Representative images of PD ‐L1 immunostaining. PD ‐L1 was immunostained on the membrane and/or the cytoplasm of the tumor cells with variable intensities: (A) weak (score 1), (B) moderate (score 2), (C) strong (score 3). (D) PD ‐L1 was immunostained only in tumor‐infiltrated immune cells. (E) PD ‐L1 expression was detected in both tumor cells and tumor‐infiltrating immune cells.
    Figure Legend Snippet: Representative images of PD ‐L1 immunostaining. PD ‐L1 was immunostained on the membrane and/or the cytoplasm of the tumor cells with variable intensities: (A) weak (score 1), (B) moderate (score 2), (C) strong (score 3). (D) PD ‐L1 was immunostained only in tumor‐infiltrated immune cells. (E) PD ‐L1 expression was detected in both tumor cells and tumor‐infiltrating immune cells.

    Techniques Used: Immunostaining, Expressing

    12) Product Images from "mRNA expression of CDH3, IGF2BP3, and BIRC5 in biliary brush cytology specimens is a useful adjunctive tool of cytology for the diagnosis of malignant biliary stricture"

    Article Title: mRNA expression of CDH3, IGF2BP3, and BIRC5 in biliary brush cytology specimens is a useful adjunctive tool of cytology for the diagnosis of malignant biliary stricture

    Journal: Medicine

    doi: 10.1097/MD.0000000000004132

    Hematoxylin–eosin and immunohistochemical stains of benign and malignant bile duct tissues (×200). A, B, Hematoxylin–eosin staining in benign and malignant tissues; C, D, CDH3 staining in benign and malignant tissues; E, F, IGF2BP3 staining in benign and malignant tissues; G, H, HOXB7 staining in benign and malignant tissues; I, J, BIRC5 staining in benign and malignant tissues; K, L, p53 staining in benign and malignant tissues. BIRC5 = baculoviral inhibitor of apoptosis repeat containing 5, CDH3 = cadherin 3, HOXB7 = homeobox B7, IGF2BP3 = insulin-like growth factor II mRNA-binding protein 3.
    Figure Legend Snippet: Hematoxylin–eosin and immunohistochemical stains of benign and malignant bile duct tissues (×200). A, B, Hematoxylin–eosin staining in benign and malignant tissues; C, D, CDH3 staining in benign and malignant tissues; E, F, IGF2BP3 staining in benign and malignant tissues; G, H, HOXB7 staining in benign and malignant tissues; I, J, BIRC5 staining in benign and malignant tissues; K, L, p53 staining in benign and malignant tissues. BIRC5 = baculoviral inhibitor of apoptosis repeat containing 5, CDH3 = cadherin 3, HOXB7 = homeobox B7, IGF2BP3 = insulin-like growth factor II mRNA-binding protein 3.

    Techniques Used: Immunohistochemistry, Staining, Binding Assay

    13) Product Images from "Heterogeneous programmed death-ligand 1 expression in gastric cancer: comparison of tissue microarrays and whole sections"

    Article Title: Heterogeneous programmed death-ligand 1 expression in gastric cancer: comparison of tissue microarrays and whole sections

    Journal: Cancer Cell International

    doi: 10.1186/s12935-020-01273-0

    PD-L1 immunohistochemical expression. An overview of an immunohistochemical staining TMA. b , c Are two cores of the TMA from the same patient. b Negative staining, and c shows positive staining. Magnification a : ×2.5; b : ×4 (×200 for insert); c : ×4 (×200 for insert)
    Figure Legend Snippet: PD-L1 immunohistochemical expression. An overview of an immunohistochemical staining TMA. b , c Are two cores of the TMA from the same patient. b Negative staining, and c shows positive staining. Magnification a : ×2.5; b : ×4 (×200 for insert); c : ×4 (×200 for insert)

    Techniques Used: Immunohistochemistry, Expressing, Staining, Negative Staining

    14) Product Images from "Tubular Adenoma of the Breast: A Clinicopathologic Study of a Series of 9 Cases"

    Article Title: Tubular Adenoma of the Breast: A Clinicopathologic Study of a Series of 9 Cases

    Journal: Clinical Medicine Insights. Pathology

    doi: 10.1177/1179555718757499

    CD34 immunostaining highlights the characteristic small amount of the stroma intermingled between the tubular structures (original magnification ×100).
    Figure Legend Snippet: CD34 immunostaining highlights the characteristic small amount of the stroma intermingled between the tubular structures (original magnification ×100).

    Techniques Used: Immunostaining

    Immunohistochemical analysis shows a prominent expression of CK5/6 (A) and p63 protein (B) by tumors cells (original magnification x200).
    Figure Legend Snippet: Immunohistochemical analysis shows a prominent expression of CK5/6 (A) and p63 protein (B) by tumors cells (original magnification x200).

    Techniques Used: Immunohistochemistry, Expressing

    15) Product Images from "DNA repair genes implicated in triple negative familial non-BRCA1/2 breast cancer predisposition"

    Article Title: DNA repair genes implicated in triple negative familial non-BRCA1/2 breast cancer predisposition

    Journal: American Journal of Cancer Research

    doi:

    Immunohistochemical detection of the RAD51D protein. Formalin-fixed, paraffin-embedded tissue sections of breast tumours of the two RAD51D mutation carriers. A. The RAD51D protein is present as in control normal breast cells (red color in cytoplasm and
    Figure Legend Snippet: Immunohistochemical detection of the RAD51D protein. Formalin-fixed, paraffin-embedded tissue sections of breast tumours of the two RAD51D mutation carriers. A. The RAD51D protein is present as in control normal breast cells (red color in cytoplasm and

    Techniques Used: Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Mutagenesis

    16) Product Images from "Reciprocal Activating Crosstalk between c-Met and Caveolin 1 Promotes Invasive Phenotype in Hepatocellular Carcinoma"

    Article Title: Reciprocal Activating Crosstalk between c-Met and Caveolin 1 Promotes Invasive Phenotype in Hepatocellular Carcinoma

    Journal: PLoS ONE

    doi: 10.1371/journal.pone.0105278

    Immunohistochemical assesment of phospho-Met and phospho-CAV1 expressions in normal, cirrhotic and HCC tissues. Negative phosphor-Met expression in normal hepatocytes (a), cirrhotic liver tissue showed weak, phospho-Met staining (b). HCC displayed intense phospho-Met staining. Each column represents histologically classified liver tissues (normal liver, cirrhotic liver, HCC) with the height representing the ratio of positive staining for phospho-Met (d). Negative phospho-CAV1 expression in normal liver tissue (e), diffuse phospho-CAV1 staining in hepatocytes in the cirrhotic liver tissue (g), HCC displayed strong phospho-CAV1 staining (h). Comparison of the ratios of positive staining for phospho-CAV1 in normal liver, cirrhotic liver, and HCC tissues. Each column represents the immunoreactivity of both phospho-Met and phospho-CAV1 in the same liver tissue samples (i). Trend in χ 2 -test was performed to determine the trend between groups (*p
    Figure Legend Snippet: Immunohistochemical assesment of phospho-Met and phospho-CAV1 expressions in normal, cirrhotic and HCC tissues. Negative phosphor-Met expression in normal hepatocytes (a), cirrhotic liver tissue showed weak, phospho-Met staining (b). HCC displayed intense phospho-Met staining. Each column represents histologically classified liver tissues (normal liver, cirrhotic liver, HCC) with the height representing the ratio of positive staining for phospho-Met (d). Negative phospho-CAV1 expression in normal liver tissue (e), diffuse phospho-CAV1 staining in hepatocytes in the cirrhotic liver tissue (g), HCC displayed strong phospho-CAV1 staining (h). Comparison of the ratios of positive staining for phospho-CAV1 in normal liver, cirrhotic liver, and HCC tissues. Each column represents the immunoreactivity of both phospho-Met and phospho-CAV1 in the same liver tissue samples (i). Trend in χ 2 -test was performed to determine the trend between groups (*p

    Techniques Used: Immunohistochemistry, Expressing, Staining

    Immunohistochemical staining in well-, moderate-, and poorly differentiated HCC tissues revealed an increasing trend towards well to poor differentiation. Representative images shows Serial sections were stained with anti-phospho-Met (a, b, c) and anti-phospho-CAV1(d, e, f) antibodies. Each column represents the ratio of positive staining for phospho-Met (g) and phospho-CAV1 (h), co-expression of phospho-Met and phospho-CAV1 (i) in well-, moderate-, and poorly-differentiated HCC. Trend in χ 2 -test was performed to determine the trend between groups (* p
    Figure Legend Snippet: Immunohistochemical staining in well-, moderate-, and poorly differentiated HCC tissues revealed an increasing trend towards well to poor differentiation. Representative images shows Serial sections were stained with anti-phospho-Met (a, b, c) and anti-phospho-CAV1(d, e, f) antibodies. Each column represents the ratio of positive staining for phospho-Met (g) and phospho-CAV1 (h), co-expression of phospho-Met and phospho-CAV1 (i) in well-, moderate-, and poorly-differentiated HCC. Trend in χ 2 -test was performed to determine the trend between groups (* p

    Techniques Used: Immunohistochemistry, Staining, Expressing

    Related Articles

    Immunohistochemistry:

    Article Title: Hypoxia Pathways and Cellular Stress Activate Pancreatic Stellate Cells: Development of an Organotypic Culture Model of Thick Slices of Normal Human Pancreas
    Article Snippet: .. Immunohistochemistry was performed with an automated immunohistochemical stainer according to the manufacturer’s guidelines (Streptavidine-peroxidase with an automate Ventana, Benchmark, USA). .. A double staining was performed using Ultraview universal alkaline phosphatase red detection and Ultraview DAB kits (Ventana, Benchmark, USA) to confirm antigen co localization in some cases.

    Article Title: Non-Hodgkin and Hodgkin lymphomas select for overexpression of BCLW
    Article Snippet: IHC for BCL2 and BCLW was performed on 4μm FFPE sections from lymphoma and control tissues following standard procedures. .. BCL2 (SP66) and BCLW (ab38629, Abcam, 1:125 dilution) staining was performed using automated immunohistochemical stainers (Ventana Medical Systems and DAKO Autostainer Plus, respectively) according to manufacturer specifications. ..

    Article Title: Activation of MEK1/2‐ERK1/2 signaling during NNK‐induced lung carcinogenesis in female A/J mice
    Article Snippet: They were housed in polycarbonate cages with white wood chips for bedding and given free access to drinking water and a basal diet (Oriental MF, Oriental Yeast Co., Ltd, Tokyo, Japan), under controlled conditions of humidity (60 ± 10%), lighting (12‐h light/dark cycle) and temperature (24 ± 2°C). .. Immunohistochemistry Single‐ and double‐immunohistochemical staining was performed with the labeled streptavidin–biotin (LSAB) method, all staining processes from deparaffinization to counterstaining with hematoxylin being accomplished using an automated immunohistochemical stainer (Ventana HX Discovery system; Ventana Medical Systems, Tucson, AZ). .. For antigen retrieval, lung tissue sections were heated in RicoCC Buffer (Ventana Medical Systems) at 95°C for 30 min.

    Article Title: Phase I Trial of Sorafenib Following Liver Transplantation in Patients with High-Risk Hepatocellular Carcinoma
    Article Snippet: The TMA block was cut in 4-µm sections and immunolabeled with antibodies directed against the following rabbit monoclonal antibodies: c-MET (SP44), prediluted, CONFIRM (Ventana, Tucson, AZ); phospho-ERK1/2 [phospho p44/42 Map kinase (Erk1/2) (Thr202 / Tyr204) (D13.14.4E), 1:300 dilution, Cell Signaling Technology, Danvers, MA]; and phospho-Akt (Ser 473) (736E11), 1:40 dilution, Cell Signaling Technology. .. Immunostaining for c-Met was carried out using an automated immunohistochemical stainer according to the manufacturer's guidelines (streptavidin-peroxidase with automated Ventana Benchmark, Ventana). .. Immunostaining for phospho-ERK1/2 and phospho-Akt were processed manually.

    Article Title: ?4-integrins control viral meningoencephalitis through differential recruitment of T helper cell subsets
    Article Snippet: IHC staining was performed on an automated Leica BOND-MAX instrument using Bond Polymer Refine Detection Solution for DAB. .. For detection of GFAP, PFA-fixed and paraffin-embedded CNS sections were incubated with Dako polyclonal rabbit anti-GFAP antibodies (Z0034; 1:13000) in Ventana buffer and staining was performed on a Ventana NexES IHC Slide Stainer using iVIEW DAB Detection Kit (Ventana). .. Images were taken using the Leica SCN400 slide scanner analysis software or were acquired on an Olympus BX53 Microscope (DP72 camera) using the cellSens 1.8 digital imaging software (Olympus).

    Article Title: γH2AX is immunohistochemically detectable until 7 days after exposure of N-bis (2-hydroxypropyl) nitrosamine (DHPN) in rat lung carcinogenesis
    Article Snippet: .. Immunohistochemistry All slides from lung tissues were deparaffinized and stained using an automated immunohistochemical stainer (Ventana HX Discovery System; Ventana Medical Systems, Tucson, AZ, USA) for γH2AX and PCNA. .. The slides were first treated with CC2 Buffer (Ventana Medical Systems) prior to immunohistochemical staining.

    Article Title: Programmed Death-ligand 1 (PD-L1) Expression in Thymic Epithelial Tumors.
    Article Snippet: .. Thymic epithelial tumors (TETs) are uncommon neoplasms of the mediastinum. .. Thymic epithelial tumors (TETs) are uncommon neoplasms of the mediastinum.

    Staining:

    Article Title: Non-Hodgkin and Hodgkin lymphomas select for overexpression of BCLW
    Article Snippet: IHC for BCL2 and BCLW was performed on 4μm FFPE sections from lymphoma and control tissues following standard procedures. .. BCL2 (SP66) and BCLW (ab38629, Abcam, 1:125 dilution) staining was performed using automated immunohistochemical stainers (Ventana Medical Systems and DAKO Autostainer Plus, respectively) according to manufacturer specifications. ..

    Article Title: Activation of MEK1/2‐ERK1/2 signaling during NNK‐induced lung carcinogenesis in female A/J mice
    Article Snippet: They were housed in polycarbonate cages with white wood chips for bedding and given free access to drinking water and a basal diet (Oriental MF, Oriental Yeast Co., Ltd, Tokyo, Japan), under controlled conditions of humidity (60 ± 10%), lighting (12‐h light/dark cycle) and temperature (24 ± 2°C). .. Immunohistochemistry Single‐ and double‐immunohistochemical staining was performed with the labeled streptavidin–biotin (LSAB) method, all staining processes from deparaffinization to counterstaining with hematoxylin being accomplished using an automated immunohistochemical stainer (Ventana HX Discovery system; Ventana Medical Systems, Tucson, AZ). .. For antigen retrieval, lung tissue sections were heated in RicoCC Buffer (Ventana Medical Systems) at 95°C for 30 min.

    Article Title: ?4-integrins control viral meningoencephalitis through differential recruitment of T helper cell subsets
    Article Snippet: IHC staining was performed on an automated Leica BOND-MAX instrument using Bond Polymer Refine Detection Solution for DAB. .. For detection of GFAP, PFA-fixed and paraffin-embedded CNS sections were incubated with Dako polyclonal rabbit anti-GFAP antibodies (Z0034; 1:13000) in Ventana buffer and staining was performed on a Ventana NexES IHC Slide Stainer using iVIEW DAB Detection Kit (Ventana). .. Images were taken using the Leica SCN400 slide scanner analysis software or were acquired on an Olympus BX53 Microscope (DP72 camera) using the cellSens 1.8 digital imaging software (Olympus).

    Article Title: γH2AX is immunohistochemically detectable until 7 days after exposure of N-bis (2-hydroxypropyl) nitrosamine (DHPN) in rat lung carcinogenesis
    Article Snippet: .. Immunohistochemistry All slides from lung tissues were deparaffinized and stained using an automated immunohistochemical stainer (Ventana HX Discovery System; Ventana Medical Systems, Tucson, AZ, USA) for γH2AX and PCNA. .. The slides were first treated with CC2 Buffer (Ventana Medical Systems) prior to immunohistochemical staining.

    Article Title: Programmed Death-ligand 1 (PD-L1) Expression in Thymic Epithelial Tumors.
    Article Snippet: .. Thymic epithelial tumors (TETs) are uncommon neoplasms of the mediastinum. .. Thymic epithelial tumors (TETs) are uncommon neoplasms of the mediastinum.

    Labeling:

    Article Title: Activation of MEK1/2‐ERK1/2 signaling during NNK‐induced lung carcinogenesis in female A/J mice
    Article Snippet: They were housed in polycarbonate cages with white wood chips for bedding and given free access to drinking water and a basal diet (Oriental MF, Oriental Yeast Co., Ltd, Tokyo, Japan), under controlled conditions of humidity (60 ± 10%), lighting (12‐h light/dark cycle) and temperature (24 ± 2°C). .. Immunohistochemistry Single‐ and double‐immunohistochemical staining was performed with the labeled streptavidin–biotin (LSAB) method, all staining processes from deparaffinization to counterstaining with hematoxylin being accomplished using an automated immunohistochemical stainer (Ventana HX Discovery system; Ventana Medical Systems, Tucson, AZ). .. For antigen retrieval, lung tissue sections were heated in RicoCC Buffer (Ventana Medical Systems) at 95°C for 30 min.

    Immunostaining:

    Article Title: Phase I Trial of Sorafenib Following Liver Transplantation in Patients with High-Risk Hepatocellular Carcinoma
    Article Snippet: The TMA block was cut in 4-µm sections and immunolabeled with antibodies directed against the following rabbit monoclonal antibodies: c-MET (SP44), prediluted, CONFIRM (Ventana, Tucson, AZ); phospho-ERK1/2 [phospho p44/42 Map kinase (Erk1/2) (Thr202 / Tyr204) (D13.14.4E), 1:300 dilution, Cell Signaling Technology, Danvers, MA]; and phospho-Akt (Ser 473) (736E11), 1:40 dilution, Cell Signaling Technology. .. Immunostaining for c-Met was carried out using an automated immunohistochemical stainer according to the manufacturer's guidelines (streptavidin-peroxidase with automated Ventana Benchmark, Ventana). .. Immunostaining for phospho-ERK1/2 and phospho-Akt were processed manually.

    Incubation:

    Article Title: ?4-integrins control viral meningoencephalitis through differential recruitment of T helper cell subsets
    Article Snippet: IHC staining was performed on an automated Leica BOND-MAX instrument using Bond Polymer Refine Detection Solution for DAB. .. For detection of GFAP, PFA-fixed and paraffin-embedded CNS sections were incubated with Dako polyclonal rabbit anti-GFAP antibodies (Z0034; 1:13000) in Ventana buffer and staining was performed on a Ventana NexES IHC Slide Stainer using iVIEW DAB Detection Kit (Ventana). .. Images were taken using the Leica SCN400 slide scanner analysis software or were acquired on an Olympus BX53 Microscope (DP72 camera) using the cellSens 1.8 digital imaging software (Olympus).

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    • immunohistochemical stainer  (Ventana Medical)
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    Ventana Medical immunohistochemical stainer
    <t>Immunohistochemical</t> assesment of phospho-Met and phospho-CAV1 expressions in normal, cirrhotic and HCC tissues. Negative phosphor-Met expression in normal hepatocytes (a), cirrhotic liver tissue showed weak, phospho-Met staining (b). HCC displayed intense phospho-Met staining. Each column represents histologically classified liver tissues (normal liver, cirrhotic liver, HCC) with the height representing the ratio of positive staining for phospho-Met (d). Negative phospho-CAV1 expression in normal liver tissue (e), diffuse phospho-CAV1 staining in hepatocytes in the cirrhotic liver tissue (g), HCC displayed strong phospho-CAV1 staining (h). Comparison of the ratios of positive staining for phospho-CAV1 in normal liver, cirrhotic liver, and HCC tissues. Each column represents the immunoreactivity of both phospho-Met and phospho-CAV1 in the same liver tissue samples (i). Trend in χ 2 -test was performed to determine the trend between groups (*p
    Immunohistochemical Stainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunohistochemical stainer/product/Ventana Medical
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    immunohistochemical stainer - by Bioz Stars, 2021-04
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    automatic immunohistochemical stainer  (Ventana Medical)
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    Ventana Medical automatic immunohistochemical stainer
    β-Catenin <t>immunostaining</t> of the pilomatricoma (×100).
    Automatic Immunohistochemical Stainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/automatic immunohistochemical stainer/product/Ventana Medical
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    ventana discovery xt automated immunohistochemical stainer  (Ventana Medical)
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    Ventana Medical ventana discovery xt automated immunohistochemical stainer
    CD10 <t>immunohistochemical</t> analysis using a tissue micro-array (original magnification, ×200). a Tumor cells are negative for CD10; b tumor cytoplasm is weakly positive for CD10; c tumor cytoplasm is strongly positive for CD10; d tumor-related stroma
    Ventana Discovery Xt Automated Immunohistochemical Stainer, supplied by Ventana Medical, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/ventana discovery xt automated immunohistochemical stainer/product/Ventana Medical
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    Image Search Results


    Immunohistochemical assesment of phospho-Met and phospho-CAV1 expressions in normal, cirrhotic and HCC tissues. Negative phosphor-Met expression in normal hepatocytes (a), cirrhotic liver tissue showed weak, phospho-Met staining (b). HCC displayed intense phospho-Met staining. Each column represents histologically classified liver tissues (normal liver, cirrhotic liver, HCC) with the height representing the ratio of positive staining for phospho-Met (d). Negative phospho-CAV1 expression in normal liver tissue (e), diffuse phospho-CAV1 staining in hepatocytes in the cirrhotic liver tissue (g), HCC displayed strong phospho-CAV1 staining (h). Comparison of the ratios of positive staining for phospho-CAV1 in normal liver, cirrhotic liver, and HCC tissues. Each column represents the immunoreactivity of both phospho-Met and phospho-CAV1 in the same liver tissue samples (i). Trend in χ 2 -test was performed to determine the trend between groups (*p

    Journal: PLoS ONE

    Article Title: Reciprocal Activating Crosstalk between c-Met and Caveolin 1 Promotes Invasive Phenotype in Hepatocellular Carcinoma

    doi: 10.1371/journal.pone.0105278

    Figure Lengend Snippet: Immunohistochemical assesment of phospho-Met and phospho-CAV1 expressions in normal, cirrhotic and HCC tissues. Negative phosphor-Met expression in normal hepatocytes (a), cirrhotic liver tissue showed weak, phospho-Met staining (b). HCC displayed intense phospho-Met staining. Each column represents histologically classified liver tissues (normal liver, cirrhotic liver, HCC) with the height representing the ratio of positive staining for phospho-Met (d). Negative phospho-CAV1 expression in normal liver tissue (e), diffuse phospho-CAV1 staining in hepatocytes in the cirrhotic liver tissue (g), HCC displayed strong phospho-CAV1 staining (h). Comparison of the ratios of positive staining for phospho-CAV1 in normal liver, cirrhotic liver, and HCC tissues. Each column represents the immunoreactivity of both phospho-Met and phospho-CAV1 in the same liver tissue samples (i). Trend in χ 2 -test was performed to determine the trend between groups (*p

    Article Snippet: Immunostaining was performed using an automated immunohistochemical stainer according to the manufacturer's guidelines (IVIEW DAB, BenchMarkXP, and Ventana, USA).

    Techniques: Immunohistochemistry, Expressing, Staining

    Immunohistochemical staining in well-, moderate-, and poorly differentiated HCC tissues revealed an increasing trend towards well to poor differentiation. Representative images shows Serial sections were stained with anti-phospho-Met (a, b, c) and anti-phospho-CAV1(d, e, f) antibodies. Each column represents the ratio of positive staining for phospho-Met (g) and phospho-CAV1 (h), co-expression of phospho-Met and phospho-CAV1 (i) in well-, moderate-, and poorly-differentiated HCC. Trend in χ 2 -test was performed to determine the trend between groups (* p

    Journal: PLoS ONE

    Article Title: Reciprocal Activating Crosstalk between c-Met and Caveolin 1 Promotes Invasive Phenotype in Hepatocellular Carcinoma

    doi: 10.1371/journal.pone.0105278

    Figure Lengend Snippet: Immunohistochemical staining in well-, moderate-, and poorly differentiated HCC tissues revealed an increasing trend towards well to poor differentiation. Representative images shows Serial sections were stained with anti-phospho-Met (a, b, c) and anti-phospho-CAV1(d, e, f) antibodies. Each column represents the ratio of positive staining for phospho-Met (g) and phospho-CAV1 (h), co-expression of phospho-Met and phospho-CAV1 (i) in well-, moderate-, and poorly-differentiated HCC. Trend in χ 2 -test was performed to determine the trend between groups (* p

    Article Snippet: Immunostaining was performed using an automated immunohistochemical stainer according to the manufacturer's guidelines (IVIEW DAB, BenchMarkXP, and Ventana, USA).

    Techniques: Immunohistochemistry, Staining, Expressing

    Immunohistochemical detection of the RAD51D protein. Formalin-fixed, paraffin-embedded tissue sections of breast tumours of the two RAD51D mutation carriers. A. The RAD51D protein is present as in control normal breast cells (red color in cytoplasm and

    Journal: American Journal of Cancer Research

    Article Title: DNA repair genes implicated in triple negative familial non-BRCA1/2 breast cancer predisposition

    doi:

    Figure Lengend Snippet: Immunohistochemical detection of the RAD51D protein. Formalin-fixed, paraffin-embedded tissue sections of breast tumours of the two RAD51D mutation carriers. A. The RAD51D protein is present as in control normal breast cells (red color in cytoplasm and

    Article Snippet: All procedures were performed in a fully automated immunohistochemical stainer (Benchmark XT, Ventana).

    Techniques: Immunohistochemistry, Formalin-fixed Paraffin-Embedded, Mutagenesis

    β-Catenin immunostaining of the pilomatricoma (×100).

    Journal: Annals of Dermatology

    Article Title: The Immunohistochemical Patterns of the ?-Catenin Expression in Pilomatricoma

    doi: 10.5021/ad.2010.22.3.284

    Figure Lengend Snippet: β-Catenin immunostaining of the pilomatricoma (×100).

    Article Snippet: The tissue sections were stained using an automatic immunohistochemical stainer (BenchMark XT, Ventana Medical System, Inc., Tucson, AZ, USA).

    Techniques: Immunostaining

    CD10 immunohistochemical analysis using a tissue micro-array (original magnification, ×200). a Tumor cells are negative for CD10; b tumor cytoplasm is weakly positive for CD10; c tumor cytoplasm is strongly positive for CD10; d tumor-related stroma

    Journal: Annals of surgical oncology

    Article Title: Tumoral CD10 Expression Correlates with Aggressive Histology and Prognosis in Patients with Malignant Pleural Mesothelioma

    doi: 10.1245/s10434-015-4374-x

    Figure Lengend Snippet: CD10 immunohistochemical analysis using a tissue micro-array (original magnification, ×200). a Tumor cells are negative for CD10; b tumor cytoplasm is weakly positive for CD10; c tumor cytoplasm is strongly positive for CD10; d tumor-related stroma

    Article Snippet: Sections were stained using a Ventana Discovery XT automated immunohistochemical stainer (Ventana, Tucson, AZ, USA) in accordance with the manufacturer’s guidelines.

    Techniques: Immunohistochemistry, Microarray