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(A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the <t>immunogenic</t> sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.
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(A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the <t>immunogenic</t> sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.
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(A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the <t>immunogenic</t> sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.
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(A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the immunogenic sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.

Journal: PLOS ONE

Article Title: PAX6 protein in neuromasts of the lateral line system of salamanders ( Eurycea )

doi: 10.1371/journal.pone.0293163

Figure Lengend Snippet: (A) An alignment of human PAX6 sequences reveals that all fifteen isoforms share the immunogenic sequence (highlighted in dark grey). NCBI sequence numbers are to the left of the sequences which are arranged alphabetically by isoform. (B) The four isoforms of axolotl PAX6 (sequences 1–4) contain the immunogenic sequence as does PAX6 from the Japanese fire-bellied newt (sequence 5) and the hypothetical protein from the Iberian ribbed newt (sequence 6). Sequence 7 is human PAX6. (C) Partial sequence obtained from E . nana containing 16/19 amino acids from the PAX6 immunogenic sequence.

Article Snippet: Primary antibody was diluted 1:10,000 in 5% non-fat dry milk in Tris-buffered saline with Tween20 (TBST), and half was incubated with custom peptide (Thermo Fisher) matching the antibody’s immunogenic sequence, resuspended in PBS at a concentration at least 5 times the molarity of the antibody.

Techniques: Sequencing