immunofluorescence nih 3t3 cells  (ATCC)


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    Name:
    NIH 3T3
    Description:

    Catalog Number:
    crl-1658
    Price:
    None
    Applications:
    This cell line is a suitable transfection host. The NIH/3T3 cell line is highly sensitive to sarcoma virus focus formation and leukemia virus propagation and has proven to be very useful in DNA transfection studies. ref
    Host:
    Mus musculus, mouse
    Cell Type:
    fibroblast
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    Structured Review

    ATCC immunofluorescence nih 3t3 cells
    The Mito tracking peptide targets proteins to the mitochondria. <t>NIH</t> <t>3T3</t> cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.

    https://www.bioz.com/result/immunofluorescence nih 3t3 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence nih 3t3 cells - by Bioz Stars, 2020-05
    99/100 stars

    Images

    1) Product Images from "Modification of the Creator recombination system for proteomics applications - improved expression by addition of splice sites"

    Article Title: Modification of the Creator recombination system for proteomics applications - improved expression by addition of splice sites

    Journal: BMC Biotechnology

    doi: 10.1186/1472-6750-6-13

    The Mito tracking peptide targets proteins to the mitochondria. NIH 3T3 cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.
    Figure Legend Snippet: The Mito tracking peptide targets proteins to the mitochondria. NIH 3T3 cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.

    Techniques Used: Transfection, Expressing, Staining, Construct

    Related Articles

    Infection:

    Article Title: Murine Cytomegalovirus m142 and m143 Are both Required To Block Protein Kinase R-Mediated Shutdown of Protein Synthesis
    Article Snippet: .. For metabolic labeling, NIH 3T3 cells were infected at a multiplicity of infection (MOI) of 5 TCID50 /cell. .. Twenty-four hours after infection, the cells were incubated for 1 h in medium containing [35 S]methionine and [35 S]cysteine (143 μCi total radioactivity).

    Transfection:

    Article Title: Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 protein
    Article Snippet: .. Cell culture and plasmids for transfection NIH3T3 (3T3-3-4, Riken Cell Bank, RRID: CVCL_1926 ) and HEK293 (HEK293T/17, ATCC, Japan, RRID: CVCL_L993 ) were authorized by Riken Cell Bank and ATCC respectively as follows. ..

    Multiple Displacement Amplification:

    Article Title: The Breast Cancer Oncogene EMSY Represses Transcription of Antimetastatic microRNA miR-31
    Article Snippet: .. Cell Culture Mouse NIH 3T3 and human breast cancer cell lines MCF-7, MDA-MB-415, and MDA-MB-175 were purchased from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin and streptomycin and grown at 37°C under 5% CO2 . .. Xenograft Assay and Tail Vein Injection In this study, 5-week-old female athymic nu/nu mice (Harlan Laboratories) housed under specific pathogen-free conditions were used.

    Labeling:

    Article Title: Murine Cytomegalovirus m142 and m143 Are both Required To Block Protein Kinase R-Mediated Shutdown of Protein Synthesis
    Article Snippet: .. For metabolic labeling, NIH 3T3 cells were infected at a multiplicity of infection (MOI) of 5 TCID50 /cell. .. Twenty-four hours after infection, the cells were incubated for 1 h in medium containing [35 S]methionine and [35 S]cysteine (143 μCi total radioactivity).

    Cell Culture:

    Article Title: Retargeting of UniCAR T cells with an in vivo synthesized target module directed against CD19 positive tumor cells
    Article Snippet: .. Human Embryonic Kidney cells HEK293T (ATCC CRL-11268) and murine 3T3 cells (ATCC CRL-1658) were cultured in DMEM medium (ThermoFisher Scientific, Schwerte, Germany) supplemented with 10% FCS, 100 µg/ml penicillin/streptomycin and 1% non-essential amino acids. ..

    Article Title: Salt-inducible kinase 3 regulates the mammalian circadian clock by destabilizing PER2 protein
    Article Snippet: .. Cell culture and plasmids for transfection NIH3T3 (3T3-3-4, Riken Cell Bank, RRID: CVCL_1926 ) and HEK293 (HEK293T/17, ATCC, Japan, RRID: CVCL_L993 ) were authorized by Riken Cell Bank and ATCC respectively as follows. ..

    Article Title: The Breast Cancer Oncogene EMSY Represses Transcription of Antimetastatic microRNA miR-31
    Article Snippet: .. Cell Culture Mouse NIH 3T3 and human breast cancer cell lines MCF-7, MDA-MB-415, and MDA-MB-175 were purchased from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin and streptomycin and grown at 37°C under 5% CO2 . .. Xenograft Assay and Tail Vein Injection In this study, 5-week-old female athymic nu/nu mice (Harlan Laboratories) housed under specific pathogen-free conditions were used.

    Electrophoretic Mobility Shift Assay:

    Article Title: Murine Cytomegalovirus m142 and m143 Are both Required To Block Protein Kinase R-Mediated Shutdown of Protein Synthesis
    Article Snippet: .. The more slowly migrating band most probably represents phosphorylated (i.e., activated) PKR, because treatment of NIH 3T3 cells with poly(I · C), a known inducer of PKR phosphorylation, resulted in a comparable band shift (data not shown). ..

    Modification:

    Article Title: The Breast Cancer Oncogene EMSY Represses Transcription of Antimetastatic microRNA miR-31
    Article Snippet: .. Cell Culture Mouse NIH 3T3 and human breast cancer cell lines MCF-7, MDA-MB-415, and MDA-MB-175 were purchased from ATCC and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum (FBS; Gibco) and 1% penicillin and streptomycin and grown at 37°C under 5% CO2 . .. Xenograft Assay and Tail Vein Injection In this study, 5-week-old female athymic nu/nu mice (Harlan Laboratories) housed under specific pathogen-free conditions were used.

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  • 99
    ATCC immunofluorescence nih 3t3 cells
    The Mito tracking peptide targets proteins to the mitochondria. <t>NIH</t> <t>3T3</t> cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.
    Immunofluorescence Nih 3t3 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence nih 3t3 cells/product/ATCC
    Average 99 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence nih 3t3 cells - by Bioz Stars, 2020-05
    99/100 stars
      Buy from Supplier

    Image Search Results


    The Mito tracking peptide targets proteins to the mitochondria. NIH 3T3 cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.

    Journal: BMC Biotechnology

    Article Title: Modification of the Creator recombination system for proteomics applications - improved expression by addition of splice sites

    doi: 10.1186/1472-6750-6-13

    Figure Lengend Snippet: The Mito tracking peptide targets proteins to the mitochondria. NIH 3T3 cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.

    Article Snippet: Immunofluorescence NIH 3T3 cells (ATCC) were plated on coverslips in 6 well dishes in DMEM media supplemented with 10% fetal calf serum (HyClone).

    Techniques: Transfection, Expressing, Staining, Construct

    Mammalian septins recognize membrane curvature. (A) Phalloidin (magenta) and SEPT7 (green) localization, visualized by α-SEPT7 immunofluorescence, in NIH 3T3 fibroblasts. (B) SEPT7 localization (green) in NIH 3T3 fibroblasts treated with 1 µM latrunculin A for 15 min to disrupt actin-dependent septin localization. The cell outline (magenta), was produced by imaging Alexa Fluor 647–conjugated wheat germ agglutinin. (C) 50 nM human septin complexes (SEPT2–SEPT6–SEPT7) labeled with NHS-Alexa Fluor 488 on supported lipid bilayer–coated silica beads. (D) Adsorption of 50 nM human septin complex on bilayer-coated beads (25% PC, 75% PI, and trace RhPE). n ≥ 67 for each size. Dunn test results: ***, P

    Journal: The Journal of Cell Biology

    Article Title: Micron-scale plasma membrane curvature is recognized by the septin cytoskeleton

    doi: 10.1083/jcb.201512029

    Figure Lengend Snippet: Mammalian septins recognize membrane curvature. (A) Phalloidin (magenta) and SEPT7 (green) localization, visualized by α-SEPT7 immunofluorescence, in NIH 3T3 fibroblasts. (B) SEPT7 localization (green) in NIH 3T3 fibroblasts treated with 1 µM latrunculin A for 15 min to disrupt actin-dependent septin localization. The cell outline (magenta), was produced by imaging Alexa Fluor 647–conjugated wheat germ agglutinin. (C) 50 nM human septin complexes (SEPT2–SEPT6–SEPT7) labeled with NHS-Alexa Fluor 488 on supported lipid bilayer–coated silica beads. (D) Adsorption of 50 nM human septin complex on bilayer-coated beads (25% PC, 75% PI, and trace RhPE). n ≥ 67 for each size. Dunn test results: ***, P

    Article Snippet: Mammalian cell culture and immunofluorescence NIH 3T3 fibroblasts (ATCC) were cultured in DMEM media and supplemented with 10% FBS, 2 mM L-glutamine, and penicillin-streptomycin.

    Techniques: Immunofluorescence, Produced, Imaging, Labeling, Adsorption