immunofluorescence colorectal cancer cell lines  (ATCC)


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    ATCC immunofluorescence colorectal cancer cell lines
    POFUT1 is overexpressed in most of cancer types especially in <t>colorectal</t> cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p
    Immunofluorescence Colorectal Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence colorectal cancer cell lines/product/ATCC
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence colorectal cancer cell lines - by Bioz Stars, 2020-05
    92/100 stars

    Related Products / Commonly Used Together

    pofut1
    hct 116
    sw620

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    1) Product Images from "POFUT1 as a Promising Novel Biomarker of Colorectal Cancer"

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer

    Journal: Cancers

    doi: 10.3390/cancers10110411

    POFUT1 is overexpressed in most of cancer types especially in colorectal cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p
    Figure Legend Snippet: POFUT1 is overexpressed in most of cancer types especially in colorectal cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p

    Techniques Used: Expressing, Two Tailed Test

    POFUT1 expression in colorectal tissues and cell lines. Immunohistochemistry analysis ( A ) shows that POFUT1 is overexpressed in tumor tissues from first colorectal cancer (CRC) stage. Immunofluorescence labeling of POFUT1 (red), performed on wild-type and POFUT1 knockdown HCT 116 and SW620 human colorectal cancer cell lines, confirmed the antibody specificity ( B ). Western blot realized on colorectal tissues ( C ) and colorectal CCD841CoN, HCT 116, HT-29 and SW620 cell lines ( D ) validate the POFUT1 overexpression in cancer samples compared to healthy samples.
    Figure Legend Snippet: POFUT1 expression in colorectal tissues and cell lines. Immunohistochemistry analysis ( A ) shows that POFUT1 is overexpressed in tumor tissues from first colorectal cancer (CRC) stage. Immunofluorescence labeling of POFUT1 (red), performed on wild-type and POFUT1 knockdown HCT 116 and SW620 human colorectal cancer cell lines, confirmed the antibody specificity ( B ). Western blot realized on colorectal tissues ( C ) and colorectal CCD841CoN, HCT 116, HT-29 and SW620 cell lines ( D ) validate the POFUT1 overexpression in cancer samples compared to healthy samples.

    Techniques Used: Expressing, Immunohistochemistry, Immunofluorescence, Labeling, Western Blot, Over Expression

    Related Articles

    Labeling:

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
    Article Snippet: .. POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C. .. After three washes with PBS, non-specific binding sites were saturated for 1 h at room temperature with a blocking solution containing 10% goat serum, 1% BSA, 0.1% Triton X-100 in PBS.

    Transfection:

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
    Article Snippet: .. POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C. .. After three washes with PBS, non-specific binding sites were saturated for 1 h at room temperature with a blocking solution containing 10% goat serum, 1% BSA, 0.1% Triton X-100 in PBS.

    Immunofluorescence:

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
    Article Snippet: .. POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C. .. After three washes with PBS, non-specific binding sites were saturated for 1 h at room temperature with a blocking solution containing 10% goat serum, 1% BSA, 0.1% Triton X-100 in PBS.

    Stable Transfection:

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer
    Article Snippet: .. POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C. .. After three washes with PBS, non-specific binding sites were saturated for 1 h at room temperature with a blocking solution containing 10% goat serum, 1% BSA, 0.1% Triton X-100 in PBS.

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  • News
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  • 92
    ATCC immunofluorescence colorectal cancer cell lines
    POFUT1 is overexpressed in most of cancer types especially in <t>colorectal</t> cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p
    Immunofluorescence Colorectal Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence colorectal cancer cell lines/product/ATCC
    Average 92 stars, based on 2 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence colorectal cancer cell lines - by Bioz Stars, 2020-05
    92/100 stars
      Buy from Supplier

    91
    ATCC immunofluorescence crc cell lines
    POFUT1 is overexpressed in most of cancer types especially in <t>colorectal</t> cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p
    Immunofluorescence Crc Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence crc cell lines/product/ATCC
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence crc cell lines - by Bioz Stars, 2020-05
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    Image Search Results


    POFUT1 is overexpressed in most of cancer types especially in colorectal cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p

    Journal: Cancers

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer

    doi: 10.3390/cancers10110411

    Figure Lengend Snippet: POFUT1 is overexpressed in most of cancer types especially in colorectal cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p

    Article Snippet: POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C.

    Techniques: Expressing, Two Tailed Test

    POFUT1 expression in colorectal tissues and cell lines. Immunohistochemistry analysis ( A ) shows that POFUT1 is overexpressed in tumor tissues from first colorectal cancer (CRC) stage. Immunofluorescence labeling of POFUT1 (red), performed on wild-type and POFUT1 knockdown HCT 116 and SW620 human colorectal cancer cell lines, confirmed the antibody specificity ( B ). Western blot realized on colorectal tissues ( C ) and colorectal CCD841CoN, HCT 116, HT-29 and SW620 cell lines ( D ) validate the POFUT1 overexpression in cancer samples compared to healthy samples.

    Journal: Cancers

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer

    doi: 10.3390/cancers10110411

    Figure Lengend Snippet: POFUT1 expression in colorectal tissues and cell lines. Immunohistochemistry analysis ( A ) shows that POFUT1 is overexpressed in tumor tissues from first colorectal cancer (CRC) stage. Immunofluorescence labeling of POFUT1 (red), performed on wild-type and POFUT1 knockdown HCT 116 and SW620 human colorectal cancer cell lines, confirmed the antibody specificity ( B ). Western blot realized on colorectal tissues ( C ) and colorectal CCD841CoN, HCT 116, HT-29 and SW620 cell lines ( D ) validate the POFUT1 overexpression in cancer samples compared to healthy samples.

    Article Snippet: POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C.

    Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Labeling, Western Blot, Over Expression