immunofluorescence assays  (ATCC)


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    Name:
    293T 17 HEK 293T 17
    Description:

    Catalog Number:
    crl-11268
    Price:
    None
    Applications:
    These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.
    Host:
    Homo sapiens, human
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    ATCC immunofluorescence assays

    https://www.bioz.com/result/immunofluorescence assays/product/ATCC
    Average 99 stars, based on 12 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence assays - by Bioz Stars, 2020-04
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    Related Articles

    Positive Control:

    Article Title: Chitosan Nanoparticles as a Mucoadhesive Drug Delivery System for Ocular Administration
    Article Snippet: The day before experiment HEK293T (human embryonic kidney cell line, ATCC® CRL-11268™) and ARPE-19 (human retinal pigment epithelial cell line, ATCC® CRL-2302™) cell lines were seeded in sterile flat-bottom 96-well tissue culture plates (Greiner, Kremsmünster, Austria) in RPMI 1640 culture medium (ThermoFisher Scientific, Paisley, UK), supplemented with 10% fetal bovine serum (ThermoFisher Scientific, Paisley, UK), 100 units/mL of penicillin G (sodium salt) (Life Technologies, Paisley, UK), 100 μg/mL of streptomycin sulfate (ThermoFisher Scientific, Paisley, UK), and 2 mM l -glutamine (ThermoFisher Scientific, Paisley, UK) at a cell density of 2 × 105 cells/mL. .. Cells were incubated for 48 h. The negative control was the culture medium, and positive control was sodium dodecyl sulfate (SDS) at 0.1 mg/mL.

    Construct:

    Article Title: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms
    Article Snippet: Cells, plasmids, antibodies HEK-293T/17 cells (ATCC CRL11268) were maintained in complete high glucose Dulbecco's Modified Eagle Medium (cDMEM) supplemented with non-essential amino acids (NEAA) and 10% heat-inactivated fetal bovine serum (ΔFBS). .. Plasmid constructs expressing LASV GPC, GP1-TM, and sGP1, and the backbone vector pcDNA3.1+zeo:intA were described elsewhere [ ].

    Incubation:

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine). .. Lysates were prepared by suspension and incubation of cells in nondenaturing lysis buffer (200 mM NaCl, 100 mM Tris–HCl, 0.5% sodium deoxycholate, 1% Triton X‐100, 0.2% sodium dodecyl sulfate, 660 μM phenylmethylsulfonyl fluoride, 100 μl protease inhibitor cocktail (Sigma‐Aldrich P8215, St. Louis, MO, USA) 100 μl phosphatase inhibitor cocktail (Sigma‐Aldrich P0044), 1,250 units Benzonase nuclease (Sigma‐Aldrich E1014).

    Article Title: An LC-MS Chemical Derivatization Method for the Measurement of Five Different One-carbon States of Cellular Tetrahydrofolate
    Article Snippet: HEK293T cells (ATCC, CRL-11268) were grown in DMEM (Cellgro, 10-017) without pyruvate and supplemented with 10% FBS (Sigma) in an incubator with 5% CO2 and ambient oxygen at 37°C. .. Cells were incubated in the isotopic labeling medium for 24 h and were switched to fresh labeling medium 2 h before extraction.

    Isotopic Labeling:

    Article Title: An LC-MS Chemical Derivatization Method for the Measurement of Five Different One-carbon States of Cellular Tetrahydrofolate
    Article Snippet: HEK293T cells (ATCC, CRL-11268) were grown in DMEM (Cellgro, 10-017) without pyruvate and supplemented with 10% FBS (Sigma) in an incubator with 5% CO2 and ambient oxygen at 37°C. .. Cells were incubated in the isotopic labeling medium for 24 h and were switched to fresh labeling medium 2 h before extraction.

    Cell Culture:

    Article Title: Legionella pneumophila Type IV Effectors YlfA and YlfB Are SNARE-Like Proteins that Form Homo- and Heteromeric Complexes and Enhance the Efficiency of Vacuole Remodeling
    Article Snippet: Paragraph title: Cell Culture ... HEK293T cells (ATCC no. CRL-11268) were maintained in Dulbecco modified Eagle’s medium supplemented with 10% FBS-HI.

    Article Title: Mechanistically Distinct Mouse Models for CRX-Associated Retinopathy
    Article Snippet: .. Transient transfection assays HEK293 cells (ATCC CRL-11268) were cultured on 60 mm plates in Dulbucco's minimum essential media (DMEM) with 10% fetal bovine serum and Penicillin/Streptomycin. .. Cells in 60% confluence were transfected with pCAGIG-NRL and pCAGIG-hCRX WT , E168d2 and R90W either alone or in combination using CaCl (0.25 M) and Boric Acid Buffered Saline (1×) pH 6.75 as previously described .

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: Paragraph title: Custom antibodies, HEK293T cell culture, and immunoblotting ... HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine).

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: .. Vero (American Type Culture Collection (ATCC), Manassas, VA, USA; CCL-81), HEK293T (ATCC, CRL-11268) and RD (ATCC, CCL-136) cells were cultured in Dulbecco's modified Eagle's medium (Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (Gibco) with 5% CO2 at 37°C. ..

    Article Title: An LC-MS Chemical Derivatization Method for the Measurement of Five Different One-carbon States of Cellular Tetrahydrofolate
    Article Snippet: Paragraph title: 2) Cell culture ... HEK293T cells (ATCC, CRL-11268) were grown in DMEM (Cellgro, 10-017) without pyruvate and supplemented with 10% FBS (Sigma) in an incubator with 5% CO2 and ambient oxygen at 37°C.

    Article Title: Role of midkine-progranulin interaction during angiogenesis of hepatocellular carcinoma
    Article Snippet: Adherent 293T cells (ATCC: CRL-11268, USA) and HepG2 cells (ATCC: HB-8065, USA) were maintained in Dulbecco’s modified Eagle’s medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA). .. Human Umbilical Vein Endothelial cells (HUVECs) (Cascade Biologics: C-003-5C) were cultured in M200 medium supplemented with Low Serum Growth Supplement (LSGS) (Cascade Biologics, USA).

    Article Title: Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells
    Article Snippet: Paragraph title: Cell culture ... HEK293T cells (ATCC# CRL-11268) were grown in DMEM with 10% FCS containing 100 IU/ml Penicillin and 100 μg/ml Streptomycin (all from GIBCO).

    Expressing:

    Article Title: Legionella pneumophila Type IV Effectors YlfA and YlfB Are SNARE-Like Proteins that Form Homo- and Heteromeric Complexes and Enhance the Efficiency of Vacuole Remodeling
    Article Snippet: Cell Culture Chinese hamster ovary cells expressing FcγRII [ ] were maintained in minimal Eagle’s medium α supplemented with 10% heat-inactivated fetal bovine serum (FBS-HI). .. HEK293T cells (ATCC no. CRL-11268) were maintained in Dulbecco modified Eagle’s medium supplemented with 10% FBS-HI.

    Article Title: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms
    Article Snippet: Cells, plasmids, antibodies HEK-293T/17 cells (ATCC CRL11268) were maintained in complete high glucose Dulbecco's Modified Eagle Medium (cDMEM) supplemented with non-essential amino acids (NEAA) and 10% heat-inactivated fetal bovine serum (ΔFBS). .. Plasmid constructs expressing LASV GPC, GP1-TM, and sGP1, and the backbone vector pcDNA3.1+zeo:intA were described elsewhere [ ].

    Modification:

    Article Title: Legionella pneumophila Type IV Effectors YlfA and YlfB Are SNARE-Like Proteins that Form Homo- and Heteromeric Complexes and Enhance the Efficiency of Vacuole Remodeling
    Article Snippet: .. HEK293T cells (ATCC no. CRL-11268) were maintained in Dulbecco modified Eagle’s medium supplemented with 10% FBS-HI. .. U937 cells (ATCC no. CRL-1593) were maintained and differentiated in RPMI 1640 medium containing l-glutamine and supplemented with 10% FBS-HI.

    Article Title: ADAR1 Interacts with PKR during Human Immunodeficiency Virus Infection of Lymphocytes and Contributes to Viral Replication
    Article Snippet: .. Astrocytoma cell line U251MG ( ) and HEK 293T (ATCC CRL-11268) cells were maintained at 37°C in 5% CO2 in Dulbecco's modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (HyClone), 2 mM l -glutamine, and 1% penicillin-streptomycin (Invitrogen). ..

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: .. HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine). .. Lysates were prepared by suspension and incubation of cells in nondenaturing lysis buffer (200 mM NaCl, 100 mM Tris–HCl, 0.5% sodium deoxycholate, 1% Triton X‐100, 0.2% sodium dodecyl sulfate, 660 μM phenylmethylsulfonyl fluoride, 100 μl protease inhibitor cocktail (Sigma‐Aldrich P8215, St. Louis, MO, USA) 100 μl phosphatase inhibitor cocktail (Sigma‐Aldrich P0044), 1,250 units Benzonase nuclease (Sigma‐Aldrich E1014).

    Article Title: N6-methyladenosine modification and METTL3 modulate enterovirus 71 replication
    Article Snippet: .. Vero (American Type Culture Collection (ATCC), Manassas, VA, USA; CCL-81), HEK293T (ATCC, CRL-11268) and RD (ATCC, CCL-136) cells were cultured in Dulbecco's modified Eagle's medium (Gibco, Gaithersburg, MD, USA) supplemented with 10% fetal bovine serum (Gibco) with 5% CO2 at 37°C. ..

    Article Title: Regulation of atypical MAP kinases ERK3 and ERK4 by the phosphatase DUSP2
    Article Snippet: .. HEK 293 cells (ATCC CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS, 2 mM L-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml). .. NCI-H1299 cells (ATCC CRL 5803) were maintained in RPMI 1640 medium supplemented with 10% FBS, 2 mM L-glutamine, penicillin (100 units/ml), and streptomycin (100 μg/ml).

    Article Title: Identification and Functional Analysis of Three Isoforms of Bovine BST-2
    Article Snippet: .. Human embryonic kidney (HEK) 293T cells (ATCC CRL-11268), HeLa cells (ATCC CCL-2), and fetal lamb kidney cells constitutively producing bovine leukemia virus (BLV), FLK-BLV cells , were maintained in Dulbecco's modified Eagle's medium (Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum and penicillin/streptomycin. .. Cloning and plasmid construction of bovine BST-2 genes MDBK cells were treated for 24 h in the presence of 1,000 units (2.4 µg/mL) of IFN-αA/D (Sigma) and then total cellular RNA was extracted from cells using an RNeasy Mini kit (Qiagen, Valencia, CA, USA).

    Article Title: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms
    Article Snippet: .. Cells, plasmids, antibodies HEK-293T/17 cells (ATCC CRL11268) were maintained in complete high glucose Dulbecco's Modified Eagle Medium (cDMEM) supplemented with non-essential amino acids (NEAA) and 10% heat-inactivated fetal bovine serum (ΔFBS). .. Vero cells (ATCC CRL 1587) were maintained in cDMEM supplemented with NEAA and 5% ΔFBS.

    Article Title: Role of midkine-progranulin interaction during angiogenesis of hepatocellular carcinoma
    Article Snippet: .. Adherent 293T cells (ATCC: CRL-11268, USA) and HepG2 cells (ATCC: HB-8065, USA) were maintained in Dulbecco’s modified Eagle’s medium (Gibco, USA) supplemented with 10% fetal bovine serum (Gibco, USA). .. Human Umbilical Vein Endothelial cells (HUVECs) (Cascade Biologics: C-003-5C) were cultured in M200 medium supplemented with Low Serum Growth Supplement (LSGS) (Cascade Biologics, USA).

    Western Blot:

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine). .. Western blotting was performed using standard laboratory procedures with Mini‐PROTEAN® Precast AnyKD™ acrylamide gels (Bio‐Rad 456‐9034) and nitrocellulose membranes (Bio‐Rad 162‐0146).

    Transfection:

    Article Title: Mechanistically Distinct Mouse Models for CRX-Associated Retinopathy
    Article Snippet: .. Transient transfection assays HEK293 cells (ATCC CRL-11268) were cultured on 60 mm plates in Dulbucco's minimum essential media (DMEM) with 10% fetal bovine serum and Penicillin/Streptomycin. .. Cells in 60% confluence were transfected with pCAGIG-NRL and pCAGIG-hCRX WT , E168d2 and R90W either alone or in combination using CaCl (0.25 M) and Boric Acid Buffered Saline (1×) pH 6.75 as previously described .

    Article Title: ADAR1 Interacts with PKR during Human Immunodeficiency Virus Infection of Lymphocytes and Contributes to Viral Replication
    Article Snippet: Paragraph title: Cells and transfections. ... Astrocytoma cell line U251MG ( ) and HEK 293T (ATCC CRL-11268) cells were maintained at 37°C in 5% CO2 in Dulbecco's modified Eagle's Medium (DMEM) supplemented with 10% fetal bovine serum (HyClone), 2 mM l -glutamine, and 1% penicillin-streptomycin (Invitrogen).

    Article Title: Regulation of atypical MAP kinases ERK3 and ERK4 by the phosphatase DUSP2
    Article Snippet: Paragraph title: Cell lines and transfection ... HEK 293 cells (ATCC CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium supplemented with 10% FBS, 2 mM L-glutamine, penicillin (100 U/ml), and streptomycin (100 μg/ml).

    Protease Inhibitor:

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine). .. Lysates were prepared by suspension and incubation of cells in nondenaturing lysis buffer (200 mM NaCl, 100 mM Tris–HCl, 0.5% sodium deoxycholate, 1% Triton X‐100, 0.2% sodium dodecyl sulfate, 660 μM phenylmethylsulfonyl fluoride, 100 μl protease inhibitor cocktail (Sigma‐Aldrich P8215, St. Louis, MO, USA) 100 μl phosphatase inhibitor cocktail (Sigma‐Aldrich P0044), 1,250 units Benzonase nuclease (Sigma‐Aldrich E1014).

    Infection:

    Article Title: Legionella pneumophila Type IV Effectors YlfA and YlfB Are SNARE-Like Proteins that Form Homo- and Heteromeric Complexes and Enhance the Efficiency of Vacuole Remodeling
    Article Snippet: HEK293T cells (ATCC no. CRL-11268) were maintained in Dulbecco modified Eagle’s medium supplemented with 10% FBS-HI. .. 48 h prior to infection, differentiation was induced with 10 ng ml-1 phorbol 12-myristate 13-acetate (PMA) (Sigma no. P1585), and cells were lifted and replated 12 hours prior to infection.

    Generated:

    Article Title: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms
    Article Snippet: Cells, plasmids, antibodies HEK-293T/17 cells (ATCC CRL11268) were maintained in complete high glucose Dulbecco's Modified Eagle Medium (cDMEM) supplemented with non-essential amino acids (NEAA) and 10% heat-inactivated fetal bovine serum (ΔFBS). .. For immunoassays, Dr. Randal Schoepp kindly provided the LASV-specific GP1 mAb L52-74-7A, which was generated against purified gamma-irradiated LASV, as previously described [ ].

    Imaging:

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: Primary antibodies were detected with IRDye conjugated secondary antibodies (LI‐COR 926‐32211 or 925‐68020, Lincoln, NE, USA), and their fluorescence was measured and imaged using the Odyssey LI‐COR Imaging System. .. HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine).

    Fluorescence:

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: Primary antibodies were detected with IRDye conjugated secondary antibodies (LI‐COR 926‐32211 or 925‐68020, Lincoln, NE, USA), and their fluorescence was measured and imaged using the Odyssey LI‐COR Imaging System. .. HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine).

    Article Title: Chitosan Nanoparticles as a Mucoadhesive Drug Delivery System for Ocular Administration
    Article Snippet: When PI does gain access to nucleic acids and intercalates them, its fluorescence increases dramatically and it is therefore used to identify membrane integrity-compromised cells. .. The day before experiment HEK293T (human embryonic kidney cell line, ATCC® CRL-11268™) and ARPE-19 (human retinal pigment epithelial cell line, ATCC® CRL-2302™) cell lines were seeded in sterile flat-bottom 96-well tissue culture plates (Greiner, Kremsmünster, Austria) in RPMI 1640 culture medium (ThermoFisher Scientific, Paisley, UK), supplemented with 10% fetal bovine serum (ThermoFisher Scientific, Paisley, UK), 100 units/mL of penicillin G (sodium salt) (Life Technologies, Paisley, UK), 100 μg/mL of streptomycin sulfate (ThermoFisher Scientific, Paisley, UK), and 2 mM l -glutamine (ThermoFisher Scientific, Paisley, UK) at a cell density of 2 × 105 cells/mL.

    Isolation:

    Article Title: Immunosuppressive Yersinia Effector YopM Binds DEAD Box Helicase DDX3 to Control Ribosomal S6 Kinase in the Nucleus of Host Cells
    Article Snippet: HEK293T cells (ATCC# CRL-11268) were grown in DMEM with 10% FCS containing 100 IU/ml Penicillin and 100 μg/ml Streptomycin (all from GIBCO). .. Human peripheral blood monocytes were isolated from buffy coats (provided by Frank Bentzien, University Medical Center Eppendorf, Hamburg, Germany) as described previously [ ].

    Labeling:

    Article Title: An LC-MS Chemical Derivatization Method for the Measurement of Five Different One-carbon States of Cellular Tetrahydrofolate
    Article Snippet: HEK293T cells (ATCC, CRL-11268) were grown in DMEM (Cellgro, 10-017) without pyruvate and supplemented with 10% FBS (Sigma) in an incubator with 5% CO2 and ambient oxygen at 37°C. .. For isotope labeling experiments, cells were plated into 6 cm plates, grown for 1 day before switching to DMEM medium with tracers as indicated ([3-13 C]-serine in place of the normal serine in the medium, or [13 C,2 H]-sodium formate added to the medium) supplemented with 10% dialyzed FBS (Sigma).

    Mouse Assay:

    Article Title: Legionella pneumophila Type IV Effectors YlfA and YlfB Are SNARE-Like Proteins that Form Homo- and Heteromeric Complexes and Enhance the Efficiency of Vacuole Remodeling
    Article Snippet: HEK293T cells (ATCC no. CRL-11268) were maintained in Dulbecco modified Eagle’s medium supplemented with 10% FBS-HI. .. Bone marrow-derived macrophages were cultured from female A/J mice (Jackson Laboratory) as described previously [ ].

    Alamar Blue Assay:

    Article Title: Chitosan Nanoparticles as a Mucoadhesive Drug Delivery System for Ocular Administration
    Article Snippet: Cell Viability The cytotoxicity was assessed using general cell viability endpoint resazurin reduction (7-hydroxy-3H-phenoxazin-3-one 10-oxide) (Alamar Blue) assay and propidium iodide (PI) dye exclusion assays [ , ]. .. The day before experiment HEK293T (human embryonic kidney cell line, ATCC® CRL-11268™) and ARPE-19 (human retinal pigment epithelial cell line, ATCC® CRL-2302™) cell lines were seeded in sterile flat-bottom 96-well tissue culture plates (Greiner, Kremsmünster, Austria) in RPMI 1640 culture medium (ThermoFisher Scientific, Paisley, UK), supplemented with 10% fetal bovine serum (ThermoFisher Scientific, Paisley, UK), 100 units/mL of penicillin G (sodium salt) (Life Technologies, Paisley, UK), 100 μg/mL of streptomycin sulfate (ThermoFisher Scientific, Paisley, UK), and 2 mM l -glutamine (ThermoFisher Scientific, Paisley, UK) at a cell density of 2 × 105 cells/mL.

    Purification:

    Article Title: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms
    Article Snippet: Cells, plasmids, antibodies HEK-293T/17 cells (ATCC CRL11268) were maintained in complete high glucose Dulbecco's Modified Eagle Medium (cDMEM) supplemented with non-essential amino acids (NEAA) and 10% heat-inactivated fetal bovine serum (ΔFBS). .. For immunoassays, Dr. Randal Schoepp kindly provided the LASV-specific GP1 mAb L52-74-7A, which was generated against purified gamma-irradiated LASV, as previously described [ ].

    Plasmid Preparation:

    Article Title: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms
    Article Snippet: Cells, plasmids, antibodies HEK-293T/17 cells (ATCC CRL11268) were maintained in complete high glucose Dulbecco's Modified Eagle Medium (cDMEM) supplemented with non-essential amino acids (NEAA) and 10% heat-inactivated fetal bovine serum (ΔFBS). .. Plasmid constructs expressing LASV GPC, GP1-TM, and sGP1, and the backbone vector pcDNA3.1+zeo:intA were described elsewhere [ ].

    Negative Control:

    Article Title: Chitosan Nanoparticles as a Mucoadhesive Drug Delivery System for Ocular Administration
    Article Snippet: The day before experiment HEK293T (human embryonic kidney cell line, ATCC® CRL-11268™) and ARPE-19 (human retinal pigment epithelial cell line, ATCC® CRL-2302™) cell lines were seeded in sterile flat-bottom 96-well tissue culture plates (Greiner, Kremsmünster, Austria) in RPMI 1640 culture medium (ThermoFisher Scientific, Paisley, UK), supplemented with 10% fetal bovine serum (ThermoFisher Scientific, Paisley, UK), 100 units/mL of penicillin G (sodium salt) (Life Technologies, Paisley, UK), 100 μg/mL of streptomycin sulfate (ThermoFisher Scientific, Paisley, UK), and 2 mM l -glutamine (ThermoFisher Scientific, Paisley, UK) at a cell density of 2 × 105 cells/mL. .. Cells were incubated for 48 h. The negative control was the culture medium, and positive control was sodium dodecyl sulfate (SDS) at 0.1 mg/mL.

    Gel Permeation Chromatography:

    Article Title: Characterization of the Lassa virus GP1 ectodomain shedding: implications for improved diagnostic platforms
    Article Snippet: Cells, plasmids, antibodies HEK-293T/17 cells (ATCC CRL11268) were maintained in complete high glucose Dulbecco's Modified Eagle Medium (cDMEM) supplemented with non-essential amino acids (NEAA) and 10% heat-inactivated fetal bovine serum (ΔFBS). .. Plasmid constructs expressing LASV GPC, GP1-TM, and sGP1, and the backbone vector pcDNA3.1+zeo:intA were described elsewhere [ ].

    Produced:

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: Custom antibodies, HEK293T cell culture, and immunoblotting Oligopeptides and phospho‐specific rabbit antibodies against TDP‐43 amino acids 40–53 (GLRYRNPVSQCMRG) were custom produced by GenScript (Piscataway, NJ USA). .. HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine).

    Lysis:

    Article Title: A single N‐terminal phosphomimic disrupts TDP‐43 polymerization, phase separation, and RNA splicing
    Article Snippet: HEK293T cells were obtained from ATCC (CRL‐11268) and maintained under standard conditions at 37°C in DMEM‐based media (Dulbecco's modified Eagle's medium) supplemented with 10% fetal bovine serum and 1% P/S/G (penicillin–streptomycin–glutamine). .. Lysates were prepared by suspension and incubation of cells in nondenaturing lysis buffer (200 mM NaCl, 100 mM Tris–HCl, 0.5% sodium deoxycholate, 1% Triton X‐100, 0.2% sodium dodecyl sulfate, 660 μM phenylmethylsulfonyl fluoride, 100 μl protease inhibitor cocktail (Sigma‐Aldrich P8215, St. Louis, MO, USA) 100 μl phosphatase inhibitor cocktail (Sigma‐Aldrich P0044), 1,250 units Benzonase nuclease (Sigma‐Aldrich E1014).

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  • 99
    ATCC immunofluorescence nih 3t3 cells
    The Mito tracking peptide targets proteins to the mitochondria. <t>NIH</t> <t>3T3</t> cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.
    Immunofluorescence Nih 3t3 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence nih 3t3 cells/product/ATCC
    Average 99 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence nih 3t3 cells - by Bioz Stars, 2020-04
    99/100 stars
      Buy from Supplier

    91
    ATCC immunofluorescence assay transfected monolayers
    Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. <t>Nod2-HA-transfected</t> HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).
    Immunofluorescence Assay Transfected Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence assay transfected monolayers/product/ATCC
    Average 91 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence assay transfected monolayers - by Bioz Stars, 2020-04
    91/100 stars
      Buy from Supplier

    95
    ATCC immunofluorescence colorectal cancer cell lines
    POFUT1 is overexpressed in most of cancer types especially in <t>colorectal</t> cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p
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    The Mito tracking peptide targets proteins to the mitochondria. NIH 3T3 cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.

    Journal: BMC Biotechnology

    Article Title: Modification of the Creator recombination system for proteomics applications - improved expression by addition of splice sites

    doi: 10.1186/1472-6750-6-13

    Figure Lengend Snippet: The Mito tracking peptide targets proteins to the mitochondria. NIH 3T3 cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.

    Article Snippet: Immunofluorescence NIH 3T3 cells (ATCC) were plated on coverslips in 6 well dishes in DMEM media supplemented with 10% fetal calf serum (HyClone).

    Techniques: Transfection, Expressing, Staining, Construct

    Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. Nod2-HA-transfected HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).

    Journal: Scientific Reports

    Article Title: Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection

    doi: 10.1038/srep29289

    Figure Lengend Snippet: Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. Nod2-HA-transfected HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).

    Article Snippet: Cell transfection and Immunofluorescence assay Transfected monolayers of HEK-Peak cells (ATCC CRL-2828) were grown in RPMI medium containing 10% fetal bovine serum (FBS; Gibco-BRL).

    Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction, Transfection, Fluorescence, Microscopy, Staining

    POFUT1 is overexpressed in most of cancer types especially in colorectal cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p

    Journal: Cancers

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer

    doi: 10.3390/cancers10110411

    Figure Lengend Snippet: POFUT1 is overexpressed in most of cancer types especially in colorectal cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p

    Article Snippet: POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C.

    Techniques: Expressing, Two Tailed Test

    POFUT1 expression in colorectal tissues and cell lines. Immunohistochemistry analysis ( A ) shows that POFUT1 is overexpressed in tumor tissues from first colorectal cancer (CRC) stage. Immunofluorescence labeling of POFUT1 (red), performed on wild-type and POFUT1 knockdown HCT 116 and SW620 human colorectal cancer cell lines, confirmed the antibody specificity ( B ). Western blot realized on colorectal tissues ( C ) and colorectal CCD841CoN, HCT 116, HT-29 and SW620 cell lines ( D ) validate the POFUT1 overexpression in cancer samples compared to healthy samples.

    Journal: Cancers

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer

    doi: 10.3390/cancers10110411

    Figure Lengend Snippet: POFUT1 expression in colorectal tissues and cell lines. Immunohistochemistry analysis ( A ) shows that POFUT1 is overexpressed in tumor tissues from first colorectal cancer (CRC) stage. Immunofluorescence labeling of POFUT1 (red), performed on wild-type and POFUT1 knockdown HCT 116 and SW620 human colorectal cancer cell lines, confirmed the antibody specificity ( B ). Western blot realized on colorectal tissues ( C ) and colorectal CCD841CoN, HCT 116, HT-29 and SW620 cell lines ( D ) validate the POFUT1 overexpression in cancer samples compared to healthy samples.

    Article Snippet: POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C.

    Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Labeling, Western Blot, Over Expression