immunofluorescence assays  (ATCC)


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    Name:
    293T 17 HEK 293T 17
    Description:

    Catalog Number:
    crl-11268
    Price:
    None
    Applications:
    These cells constitutively express the simian virus 40 (SV40) large T antigen, and clone 17 was selected specifically for its high transfectability.
    Host:
    Homo sapiens, human
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    Structured Review

    ATCC immunofluorescence assays

    https://www.bioz.com/result/immunofluorescence assays/product/ATCC
    Average 99 stars, based on 16 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence assays - by Bioz Stars, 2020-07
    99/100 stars

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    Related Articles

    Co-Immunoprecipitation Assay:

    Article Title: Regulation of Tumor Suppressor p53 and HCT116 Cell Physiology by Histone Demethylase JMJD2D/KDM4D
    Article Snippet: .. Coimmunoprecipitation Assay Human embryonal kidney HEK293T cells (American Type Culture Collection CRL-11268) were seeded into 6-cm dishes coated with poly-L-lysine and grown in DMEM media supplemented with 10% fetal bovine serum, 100 u/ml penicillin and 100 µg/ml streptomycin at 37°C in a humidified atmosphere containing 10% CO2 . .. At 25% confluency, cells were transiently transfected by the calcium phosphate coprecipitation method with 5.5 µg pBluescript KS+ (Stratagene), 1.5 µg empty pcDNA3 vector or pcDNA3-p53, and 2 µg empty pEV3S vector or Flag-tagged expression plasmid for JMJD2D, HSPBAP1 , SMCX , JHDM1A or JMJD6 .

    Stable Transfection:

    Article Title: Heterologous Expression of Equine CYP3A94 and Investigation of a Tunable System to Regulate Co-Expressed NADPH P450 Oxidoreductase Levels
    Article Snippet: .. Production of two stable cell lines V79-CYP3A94 and V79-CYP3A94/DD-POR HEK T293 cells (CRL-11268, ATCC) were cultivated in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal calf serum, 4 mM L-glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin. .. V79 cells (a kind gift of J. Buters, TU München, Germany; ) were cultivated in DMEM supplemented with 10% fetal calf serum, 1 mM sodium pyruvate, 4 mM L-glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin (all reagents were purchased from Gibco, Carlsbad, CA, USA).

    Article Title: Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation
    Article Snippet: .. HEK293 cells (CRL-11268; ATCC), HEK293–derived HEK293A cells containing a stably integrated copy of the E1 gene (R70507; Thermo Fisher), HeLa (human cervical adenocarcinoma) cells (CCL-2; ATCC), telomerase-immortalized human mesenchymal stem cells (hMSC-TERT) , and HEK293-derived Hana3A cells engineered for constitutive expression of RTP1, RTP2, REEP1, and Gαoλϕ ( ) were cultured in DMEM (catalog. no. 31600-083; Gibco) supplemented with 10% (vol/vol) FBS (catalog no. 04-001-1C; Biological Industries) and 1% (vol/vol) penicillin/streptomycin solution (catalog. no. L0022-100; Biowest). .. All cell types were cultured at 37 °C in a humidified atmosphere containing 5% CO2 and were regularly tested for the absence of Mycoplasma and bacterial contamination.

    Cell Culture:

    Article Title: BoHV-4-based vector delivering Ebola virus surface glycoprotein
    Article Snippet: .. Cell lines Bovine embryo kidney [(BEK) were from Dr. M. Ferrari, Istituto Zooprofilattico Sperimentale, Brescia, Italy; (BS CL-94)], BEK expressing cre recombinase (BEK cre) [ ] and human embryo kidney 293T [(HEK 293T) ATCC: CRL-11268] cell lines were cultured in complete growth medium Eagle’s minimal essential medium (EMEM, LONZA) containing 10% fetal bovine serum (FBS), 2 mM of l -glutamine (SIGMA), 100 IU/mL of penicillin (SIGMA), 100 μg/mL of streptomycin (SIGMA) and 2.5 μg/mL of Amphotericin B (SIGMA) and incubated at 37 °C, 5% CO2 . .. Constructs Synthetic Zaire Ebola virus Mayinga glycoprotein (GP) ORF, tagged at the carboxy-terminal with gD106 peptide (syEBOVgD106) was excised from pUC57sy EBOVgD106 (EUROFINS, GENOMICS) with Nhe I and Sma I enzymes and the 2153 bp fragment was inserted inside Nhe I/Sma I cut pINT2EGFPTK shuttle vector [ ] to generate pINT2CMVsyEBOVgD106.

    Article Title: Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation
    Article Snippet: .. HEK293 cells (CRL-11268; ATCC), HEK293–derived HEK293A cells containing a stably integrated copy of the E1 gene (R70507; Thermo Fisher), HeLa (human cervical adenocarcinoma) cells (CCL-2; ATCC), telomerase-immortalized human mesenchymal stem cells (hMSC-TERT) , and HEK293-derived Hana3A cells engineered for constitutive expression of RTP1, RTP2, REEP1, and Gαoλϕ ( ) were cultured in DMEM (catalog. no. 31600-083; Gibco) supplemented with 10% (vol/vol) FBS (catalog no. 04-001-1C; Biological Industries) and 1% (vol/vol) penicillin/streptomycin solution (catalog. no. L0022-100; Biowest). .. All cell types were cultured at 37 °C in a humidified atmosphere containing 5% CO2 and were regularly tested for the absence of Mycoplasma and bacterial contamination.

    Incubation:

    Article Title: BoHV-4-based vector delivering Ebola virus surface glycoprotein
    Article Snippet: .. Cell lines Bovine embryo kidney [(BEK) were from Dr. M. Ferrari, Istituto Zooprofilattico Sperimentale, Brescia, Italy; (BS CL-94)], BEK expressing cre recombinase (BEK cre) [ ] and human embryo kidney 293T [(HEK 293T) ATCC: CRL-11268] cell lines were cultured in complete growth medium Eagle’s minimal essential medium (EMEM, LONZA) containing 10% fetal bovine serum (FBS), 2 mM of l -glutamine (SIGMA), 100 IU/mL of penicillin (SIGMA), 100 μg/mL of streptomycin (SIGMA) and 2.5 μg/mL of Amphotericin B (SIGMA) and incubated at 37 °C, 5% CO2 . .. Constructs Synthetic Zaire Ebola virus Mayinga glycoprotein (GP) ORF, tagged at the carboxy-terminal with gD106 peptide (syEBOVgD106) was excised from pUC57sy EBOVgD106 (EUROFINS, GENOMICS) with Nhe I and Sma I enzymes and the 2153 bp fragment was inserted inside Nhe I/Sma I cut pINT2EGFPTK shuttle vector [ ] to generate pINT2CMVsyEBOVgD106.

    Expressing:

    Article Title: BoHV-4-based vector delivering Ebola virus surface glycoprotein
    Article Snippet: .. Cell lines Bovine embryo kidney [(BEK) were from Dr. M. Ferrari, Istituto Zooprofilattico Sperimentale, Brescia, Italy; (BS CL-94)], BEK expressing cre recombinase (BEK cre) [ ] and human embryo kidney 293T [(HEK 293T) ATCC: CRL-11268] cell lines were cultured in complete growth medium Eagle’s minimal essential medium (EMEM, LONZA) containing 10% fetal bovine serum (FBS), 2 mM of l -glutamine (SIGMA), 100 IU/mL of penicillin (SIGMA), 100 μg/mL of streptomycin (SIGMA) and 2.5 μg/mL of Amphotericin B (SIGMA) and incubated at 37 °C, 5% CO2 . .. Constructs Synthetic Zaire Ebola virus Mayinga glycoprotein (GP) ORF, tagged at the carboxy-terminal with gD106 peptide (syEBOVgD106) was excised from pUC57sy EBOVgD106 (EUROFINS, GENOMICS) with Nhe I and Sma I enzymes and the 2153 bp fragment was inserted inside Nhe I/Sma I cut pINT2EGFPTK shuttle vector [ ] to generate pINT2CMVsyEBOVgD106.

    Article Title: Synthetic far-red light-mediated CRISPR-dCas9 device for inducing functional neuronal differentiation
    Article Snippet: .. HEK293 cells (CRL-11268; ATCC), HEK293–derived HEK293A cells containing a stably integrated copy of the E1 gene (R70507; Thermo Fisher), HeLa (human cervical adenocarcinoma) cells (CCL-2; ATCC), telomerase-immortalized human mesenchymal stem cells (hMSC-TERT) , and HEK293-derived Hana3A cells engineered for constitutive expression of RTP1, RTP2, REEP1, and Gαoλϕ ( ) were cultured in DMEM (catalog. no. 31600-083; Gibco) supplemented with 10% (vol/vol) FBS (catalog no. 04-001-1C; Biological Industries) and 1% (vol/vol) penicillin/streptomycin solution (catalog. no. L0022-100; Biowest). .. All cell types were cultured at 37 °C in a humidified atmosphere containing 5% CO2 and were regularly tested for the absence of Mycoplasma and bacterial contamination.

    Modification:

    Article Title: Increasing the Safety Profile of the Master Donor Live Attenuated Influenza Vaccine
    Article Snippet: .. Cells and Viruses Human embryonic kidney 293T (ATCC CRL-11268), human lung epithelial carcinoma A549 (ATCC CCL-185), and Madin-Darby canine kidney, MDCK (ATCC CCL-34) cells were grown and maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Mediatech, Inc.) supplemented with 5% fetal bovine serum (FBS) (Atlanta Biologicals) and 1% penicillin (100 U/mL)–streptomycin (100 μg/mL)–2 mM L -glutamine, P-S-G (Mediatech, Inc.) at 37 °C in air enriched with 5% CO2 . ..

    Article Title: The 5′ Untranslated Region of Human Bocavirus Capsid Transcripts Regulates Viral mRNA Biogenesis and Alternative Translation
    Article Snippet: .. The human embryonic kidney cell line HEK293T (ATCC, CRL-11268) was maintained in Dulbecco's modified Eagle medium (DMEM; Invitrogen) supplemented with 10% fetal bovine serum at 37°C in a humidified incubator with 5% CO2 . .. Four micrograms of plasmids was transfected by use of the Lipofectamine 2000 reagent (Invitrogen) into HEK293T cells cultured in 60-mm dishes according to the manufacturer's instructions.

    Article Title: Influence of untranslated regions on retroviral mRNA transfer and expression
    Article Snippet: .. Cells The 293FT cells (derived from the clone ATCC CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (GlutaMAX) supplemented with pyruvate, penicillin (100 U/ml), streptomycin (100 μg/ml), 10% heat inactivated fetal calf serum and 1% non-essential amino acids. .. Assessment of mRNA stability 293FT cells were seeded at 200,000 cells per well into 24-well plates and grown overnight at 37°C, then transfected with 200 ng of each construct using the Fugene 6 transfection reagent (Roche).

    Article Title: Heterologous Expression of Equine CYP3A94 and Investigation of a Tunable System to Regulate Co-Expressed NADPH P450 Oxidoreductase Levels
    Article Snippet: .. Production of two stable cell lines V79-CYP3A94 and V79-CYP3A94/DD-POR HEK T293 cells (CRL-11268, ATCC) were cultivated in Dulbecco's modified Eagle medium (DMEM) supplemented with 10% fetal calf serum, 4 mM L-glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin. .. V79 cells (a kind gift of J. Buters, TU München, Germany; ) were cultivated in DMEM supplemented with 10% fetal calf serum, 1 mM sodium pyruvate, 4 mM L-glutamine, 100 U/ml penicillin and 100 µg/ml streptomycin (all reagents were purchased from Gibco, Carlsbad, CA, USA).

    Derivative Assay:

    Article Title: Influence of untranslated regions on retroviral mRNA transfer and expression
    Article Snippet: .. Cells The 293FT cells (derived from the clone ATCC CRL-11268) were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (GlutaMAX) supplemented with pyruvate, penicillin (100 U/ml), streptomycin (100 μg/ml), 10% heat inactivated fetal calf serum and 1% non-essential amino acids. .. Assessment of mRNA stability 293FT cells were seeded at 200,000 cells per well into 24-well plates and grown overnight at 37°C, then transfected with 200 ng of each construct using the Fugene 6 transfection reagent (Roche).

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  • 99
    ATCC immunofluorescence nih 3t3 cells
    The Mito tracking peptide targets proteins to the mitochondria. <t>NIH</t> <t>3T3</t> cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.
    Immunofluorescence Nih 3t3 Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    90
    ATCC immunofluorescence assay transfected monolayers
    Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. <t>Nod2-HA-transfected</t> HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).
    Immunofluorescence Assay Transfected Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    92
    ATCC immunofluorescence colorectal cancer cell lines
    POFUT1 is overexpressed in most of cancer types especially in <t>colorectal</t> cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p
    Immunofluorescence Colorectal Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 92/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    The Mito tracking peptide targets proteins to the mitochondria. NIH 3T3 cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.

    Journal: BMC Biotechnology

    Article Title: Modification of the Creator recombination system for proteomics applications - improved expression by addition of splice sites

    doi: 10.1186/1472-6750-6-13

    Figure Lengend Snippet: The Mito tracking peptide targets proteins to the mitochondria. NIH 3T3 cells were transiently transfected with vectors expressing either the N-terminus of Angiomotin fused to a C-terminal ECFP (top panel) or the same protein fused to a C-terminal ECFP plus a mitochondrial tracking peptide (ECFP-mito) (bottom panel). The cells were fixed and stained with anti-ECFP (green colour in merge) that recognizes the fusion construct or anti-Cytochrome C (red colour in merge) to mark the mitochondrial membrane. DNA is stained with Hoescht (blue colour in merge). The bottom right image on both panels is a composite of all three stainings. Note that only transfected cells will display ECFP staining. Abbreviations – mito – Mito tracker, Cyt C – Cytochrome C, ECFP – enhanced cyano fluorescent protein.

    Article Snippet: Immunofluorescence NIH 3T3 cells (ATCC) were plated on coverslips in 6 well dishes in DMEM media supplemented with 10% fetal calf serum (HyClone).

    Techniques: Transfection, Expressing, Staining, Construct

    Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. Nod2-HA-transfected HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).

    Journal: Scientific Reports

    Article Title: Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection

    doi: 10.1038/srep29289

    Figure Lengend Snippet: Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. Nod2-HA-transfected HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).

    Article Snippet: Cell transfection and Immunofluorescence assay Transfected monolayers of HEK-Peak cells (ATCC CRL-2828) were grown in RPMI medium containing 10% fetal bovine serum (FBS; Gibco-BRL).

    Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction, Transfection, Fluorescence, Microscopy, Staining

    POFUT1 is overexpressed in most of cancer types especially in colorectal cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p

    Journal: Cancers

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer

    doi: 10.3390/cancers10110411

    Figure Lengend Snippet: POFUT1 is overexpressed in most of cancer types especially in colorectal cancer from the first stage. RNAseq data from FireBrowse database show that in 22 cancer types (including COAD and READ), POFUT1 expression is higher than in the corresponding normal tissues and for 6 cancer types it is the reverse ( A ). Data are missing for nine cancer types. ACC: adrenocortical carcinoma, BLCA: bladder urothelial Carcinoma, BRCA: breast invasive carcinoma, CESC: cervical squamous cell carcinoma and endocervical adenocarcinoma, CHOL: cholangiocarcinoma, COAD: colon adenocarcinoma, COADREAD: colorectal adenocarcinoma, DLBC: lymphoid neoplasm diffuse large B-cell lymphoma, ESCA: esophageal carcinoma, GBM: glioblastoma multiforme, GBMLGG: glioma, HNSC: head and neck squamous cell carcinoma, KICH: kidney chromophobe, KIPAN: pan-kidney cohort, KIRC: kidney renal clear cell carcinoma, KIRP: kidney renal papillary cell carcinoma, LAML: acute myeloid leukemia, LGG: brain lower grade glioma, LIHC: liver hepatocellular carcinoma, LUAD: lung adenocarcinoma, LUSC: lung squamous cell carcinoma, MESO: mesothelioma, OV: ovarian serous cystadenocarcinoma, PAAD: pancreatic adenocarcinoma, PCPG: pheochromocytoma and paraganglioma, PRAD: prostate adenocarcinoma, READ: rectum adenocarcinoma, SARC: sarcoma, SKCM: skin Cutaneous Melanoma, STAD: stomach adenocarcinoma, STES: stomach and esophageal carcinoma, TGCT: testicular germ cell tumors, THCA: thyroid carcinoma, THYM: Thymoma, UCEC: uterine corpus endometrial carcinoma, UCS: uterine carcinosarcoma, UVM: uveal melanoma. COADREAD RNAseq data extracted from FireBrowse database containing 626 CRC and 51 normal adjacent tissues show that POFUT1 is significantly overexpressed in tumor tissues ( B ) and from the first stage of tumor classification ( C ). For B. and C., bar graph represented mean of log2 RSEM ± SEM. Statistical significance was assessed using a two-tailed Student test; * p

    Article Snippet: POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C.

    Techniques: Expressing, Two Tailed Test

    POFUT1 expression in colorectal tissues and cell lines. Immunohistochemistry analysis ( A ) shows that POFUT1 is overexpressed in tumor tissues from first colorectal cancer (CRC) stage. Immunofluorescence labeling of POFUT1 (red), performed on wild-type and POFUT1 knockdown HCT 116 and SW620 human colorectal cancer cell lines, confirmed the antibody specificity ( B ). Western blot realized on colorectal tissues ( C ) and colorectal CCD841CoN, HCT 116, HT-29 and SW620 cell lines ( D ) validate the POFUT1 overexpression in cancer samples compared to healthy samples.

    Journal: Cancers

    Article Title: POFUT1 as a Promising Novel Biomarker of Colorectal Cancer

    doi: 10.3390/cancers10110411

    Figure Lengend Snippet: POFUT1 expression in colorectal tissues and cell lines. Immunohistochemistry analysis ( A ) shows that POFUT1 is overexpressed in tumor tissues from first colorectal cancer (CRC) stage. Immunofluorescence labeling of POFUT1 (red), performed on wild-type and POFUT1 knockdown HCT 116 and SW620 human colorectal cancer cell lines, confirmed the antibody specificity ( B ). Western blot realized on colorectal tissues ( C ) and colorectal CCD841CoN, HCT 116, HT-29 and SW620 cell lines ( D ) validate the POFUT1 overexpression in cancer samples compared to healthy samples.

    Article Snippet: POFUT1 Labeling by Immunofluorescence Colorectal cancer cell lines, HCT 116 and SW620 obtained from ATCC and the stably transfected cell lines, HCT 116 shPOFUT1 and SW620 shPOFUT1 , created by our team were fixed with 4% paraformaldehyde in PBS for 30 min at room temperature and permeabilized with HEPES Triton buffer (20 mM HEPES, 300 mM sucrose, 50 mM NaCl, 3 mM MgCl2, 0.5% Triton X-100, pH 7.4) for 30 min at 4 °C.

    Techniques: Expressing, Immunohistochemistry, Immunofluorescence, Labeling, Western Blot, Over Expression