immunofluorescence assay transfected monolayers  (ATCC)


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    ATCC immunofluorescence assay transfected monolayers
    Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. <t>Nod2-HA-transfected</t> HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).
    Immunofluorescence Assay Transfected Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence assay transfected monolayers/product/ATCC
    Average 90 stars, based on 1 article reviews
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    immunofluorescence assay transfected monolayers - by Bioz Stars, 2020-05
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    Related Products / Commonly Used Together

    rpmi medium
    cell transfection
    hek-peak cells

    Images

    1) Product Images from "Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection"

    Article Title: Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection

    Journal: Scientific Reports

    doi: 10.1038/srep29289

    Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. Nod2-HA-transfected HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).
    Figure Legend Snippet: Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. Nod2-HA-transfected HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).

    Techniques Used: Infection, Expressing, Real-time Polymerase Chain Reaction, Transfection, Fluorescence, Microscopy, Staining

    Related Articles

    Transfection:

    Article Title: Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection
    Article Snippet: .. Cell transfection and Immunofluorescence assay Transfected monolayers of HEK-Peak cells (ATCC CRL-2828) were grown in RPMI medium containing 10% fetal bovine serum (FBS; Gibco-BRL). .. For all transfection studies, HEK293A were plated at 5.104 cells/well in 24-well plates.

    Immunofluorescence:

    Article Title: Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection
    Article Snippet: .. Cell transfection and Immunofluorescence assay Transfected monolayers of HEK-Peak cells (ATCC CRL-2828) were grown in RPMI medium containing 10% fetal bovine serum (FBS; Gibco-BRL). .. For all transfection studies, HEK293A were plated at 5.104 cells/well in 24-well plates.

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    ATCC immunofluorescence assay transfected monolayers
    Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. <t>Nod2-HA-transfected</t> HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).
    Immunofluorescence Assay Transfected Monolayers, supplied by ATCC, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/immunofluorescence assay transfected monolayers/product/ATCC
    Average 90 stars, based on 1 article reviews
    Price from $9.99 to $1999.99
    immunofluorescence assay transfected monolayers - by Bioz Stars, 2020-05
    90/100 stars
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    Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. Nod2-HA-transfected HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).

    Journal: Scientific Reports

    Article Title: Nucleotide-binding oligomerization domain-containing protein 2 prompts potent inflammatory stimuli during Neospora caninum infection

    doi: 10.1038/srep29289

    Figure Lengend Snippet: Nod2 is expressed and recruited to the vacuole during N. caninum infection. The expression of Nod2 following infection of WT BMDMs with N. caninum tachyzoites (MOI 0.2) was evaluated 6 h post-infection by Real time PCR ( A ). Results were represented as relative expression of the target gene, with CT data normalized by the expression of Gapdh and are shown as means of biological triplicates and the data are representative of two independent experiments. *Statistical difference (P ≤ 0.05) between naïve and N. caninum infected WT BMDMs. Nod2-HA-transfected HEK293 cells were infected with N. caninum tachyzoites (NcT; MOI 1) and the recruitment of this receptor to the parasitophorous vacuole was evaluated by fluorescence microscopy 24 h post-infection ( B ). Besides Nod2-HA detection (green), uninfected and NcT exposed, control HEK293 and HEK293-Nod2-HA+ cells were also stained with a monoclonal antibody against the secreted GRA2 protein of N. caninum (red) and DAPI (blue). The original images were obtained with 400x magnification (white scales bars = 10 μm).

    Article Snippet: Cell transfection and Immunofluorescence assay Transfected monolayers of HEK-Peak cells (ATCC CRL-2828) were grown in RPMI medium containing 10% fetal bovine serum (FBS; Gibco-BRL).

    Techniques: Infection, Expressing, Real-time Polymerase Chain Reaction, Transfection, Fluorescence, Microscopy, Staining